Nothing Special   »   [go: up one dir, main page]

GR3035719T3 - Immortalized human skin cell lines and serum-free medium for the production thereof - Google Patents

Immortalized human skin cell lines and serum-free medium for the production thereof

Info

Publication number
GR3035719T3
GR3035719T3 GR20010400570T GR20010400570T GR3035719T3 GR 3035719 T3 GR3035719 T3 GR 3035719T3 GR 20010400570 T GR20010400570 T GR 20010400570T GR 20010400570 T GR20010400570 T GR 20010400570T GR 3035719 T3 GR3035719 T3 GR 3035719T3
Authority
GR
Greece
Prior art keywords
cell lines
serum
human skin
production
free medium
Prior art date
Application number
GR20010400570T
Other languages
English (en)
Inventor
Markus Baur
Catherine Mace
Armand Malnoe
Andrea M A Pfeifer
Marcelle Regnier
Original Assignee
Nestle Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nestle Sa filed Critical Nestle Sa
Publication of GR3035719T3 publication Critical patent/GR3035719T3/el

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
    • C12N5/0629—Keratinocytes; Whole skin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/40—Nucleotides, nucleosides or bases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/46—Amines, e.g. putrescine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/11—Epidermal growth factor [EGF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/39—Steroid hormones
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/80—Neurotransmitters; Neurohormones
    • C12N2501/81—Adrenaline
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/04—Immortalised cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Dermatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
GR20010400570T 1995-12-21 2001-04-06 Immortalized human skin cell lines and serum-free medium for the production thereof GR3035719T3 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US57648395A 1995-12-21 1995-12-21

Publications (1)

Publication Number Publication Date
GR3035719T3 true GR3035719T3 (en) 2001-07-31

Family

ID=24304605

Family Applications (1)

Application Number Title Priority Date Filing Date
GR20010400570T GR3035719T3 (en) 1995-12-21 2001-04-06 Immortalized human skin cell lines and serum-free medium for the production thereof

Country Status (23)

Country Link
US (2) US6423540B2 (el)
EP (2) EP0877797A1 (el)
JP (1) JP4404965B2 (el)
KR (1) KR100455006B1 (el)
CN (1) CN1154717C (el)
AT (1) ATE199390T1 (el)
AU (1) AU730222B2 (el)
BR (1) BR9612256B1 (el)
CA (1) CA2234118C (el)
CZ (1) CZ297289B6 (el)
DE (1) DE69611894T2 (el)
DK (1) DK0780469T3 (el)
ES (1) ES2155166T3 (el)
GR (1) GR3035719T3 (el)
HU (1) HU226195B1 (el)
IL (1) IL124191A (el)
NO (1) NO326190B1 (el)
NZ (1) NZ325814A (el)
PL (1) PL195108B1 (el)
PT (1) PT780469E (el)
RU (1) RU2215030C2 (el)
SK (1) SK283314B6 (el)
WO (1) WO1997023602A1 (el)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE69611894T2 (de) * 1995-12-21 2001-09-13 Societe Des Produits Nestle S.A., Vevey Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung
JP4671504B2 (ja) * 1998-04-17 2011-04-20 ソシエテ・デ・プロデュイ・ネスレ・エス・アー 正常ヒト皮膚組織由来の不死化した細胞系
US6964869B2 (en) 1998-07-13 2005-11-15 Wisconsin Alumni Research Foundation Method and composition for skin grafts
CN1087778C (zh) * 1999-03-02 2002-07-17 华东理工大学 杂交瘤细胞无血清培养基
DE19912798C1 (de) * 1999-03-10 2000-02-17 Andreas Jordan Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en) 2001-02-07 2001-03-08 McComb Foundation, Inc., The Cell suspension preparation technique and device
DE60221519T2 (de) * 2001-04-24 2008-04-17 Wisconsin Allumni Research Foundation, Madison Verfahren und zusammensetzung für hauttransplantate
US7262174B2 (en) 2001-05-09 2007-08-28 Geron Corporation Treatment for wounds
US20030022369A1 (en) * 2001-05-18 2003-01-30 Helen Fillmore Differentiation of specialized dermal and epidermal cells into neuronal cells
CA2452660A1 (en) * 2001-07-06 2003-01-16 Lipomics Technologies, Inc. Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en) 2002-04-08 2002-05-22 Lonza Biologics Plc Method of culturing animal cells
US20050025737A1 (en) * 2003-07-30 2005-02-03 Sebagh Jean Louis Compositions containing melon extracts
WO2005028626A2 (en) * 2003-09-18 2005-03-31 Raven Biotechnologies, Inc. Cell culture media
WO2005071065A1 (en) * 2004-01-26 2005-08-04 Cognis France S.A.S Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
US9295543B2 (en) 2005-03-17 2016-03-29 Stratatech Corporation Skin substitutes with improved purity
DE602007008627D1 (de) * 2006-02-14 2010-10-07 Genetix Ltd Zellkulturmedium
WO2010065907A2 (en) * 2008-12-05 2010-06-10 The United States Of America As Represented By The Secretary, Department Of Health And Human Services Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
US20120142103A1 (en) * 2009-05-18 2012-06-07 Kohji Nishida Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells
KR101768632B1 (ko) * 2011-05-18 2017-08-17 (주)아모레퍼시픽 칼슘 펄스를 통한 배양상피세포층의 분리방법
AU2012283667B2 (en) * 2011-07-12 2015-07-09 Foodchek Systems, Inc. Culture medium, method for culturing Salmonella and E. coli and method for detecting Salmonella and E. coli
WO2013129885A1 (ko) * 2012-02-28 2013-09-06 건국대학교 산학협력단 세포 배양액
WO2014142038A1 (ja) 2013-03-11 2014-09-18 Ｊｃｒファーマ株式会社 ヒト角膜上皮シートの製造法
AU2013205148B2 (en) 2013-03-14 2014-10-30 AVITA Medical Americas, LLC Systems and methods for tissue processing and preparation of cell suspension therefrom
WO2016085304A1 (ko) * 2014-11-28 2016-06-02 (주)아모레퍼시픽 멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
GB2547605A (en) * 2015-01-26 2017-08-23 Halliburton Energy Services Inc Rotating superhard cutting element
CN105238739A (zh) * 2015-11-11 2016-01-13 朱宁文 临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) * 2016-12-24 2017-03-22 严志海 一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) * 2017-05-12 2017-09-01 青岛赛欧凯普图乐生物医药有限公司 一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) * 2020-07-31 2022-02-18 上海尚瑞生物医药科技有限公司 一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) * 2020-11-27 2023-09-26 (주)엑셀세라퓨틱스 칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4940666A (en) * 1983-07-15 1990-07-10 University Patents, Inc. Process and defined medium for growth of human epidermal keratinocyte cells
US4885238A (en) 1987-10-30 1989-12-05 The United States Of America As Represented By The Department Of Health And Human Services Immortalized human bronchial epitherial mesothelial cell lines
US5292655A (en) 1990-01-29 1994-03-08 Wille Jr John J Method for the formation of a histologically-complete skin substitute
US5376542A (en) 1992-04-27 1994-12-27 Georgetown University Method for producing immortalized cell lines using human papilluma virus genes
AU2385795A (en) * 1994-04-12 1995-10-30 Alza Corporation Screening methods for integumental inflammation modulating agents
DE19617261A1 (de) * 1995-04-21 1996-11-28 Naeher Helmut Dr Med Priv Doz Hautverträglichkeitstest
DE69611894T2 (de) * 1995-12-21 2001-09-13 Societe Des Produits Nestle S.A., Vevey Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung

Also Published As

Publication number Publication date
PL327320A1 (en) 1998-12-07
SK283314B6 (sk) 2003-05-02
KR100455006B1 (ko) 2005-01-13
PT780469E (pt) 2001-06-29
NZ325814A (en) 2000-10-27
NO982810L (no) 1998-08-21
US6949381B2 (en) 2005-09-27
EP0780469A1 (fr) 1997-06-25
NO982810D0 (no) 1998-06-18
HUP9903742A2 (hu) 2000-03-28
HU226195B1 (hu) 2008-06-30
US20020012993A1 (en) 2002-01-31
CA2234118A1 (en) 1997-07-03
BR9612256B1 (pt) 2010-08-10
AU1305497A (en) 1997-07-17
EP0780469B1 (fr) 2001-02-28
CN1205737A (zh) 1999-01-20
RU2215030C2 (ru) 2003-10-27
US6423540B2 (en) 2002-07-23
JP4404965B2 (ja) 2010-01-27
PL195108B1 (pl) 2007-08-31
US20020042129A1 (en) 2002-04-11
ES2155166T3 (es) 2001-05-01
DE69611894T2 (de) 2001-09-13
DK0780469T3 (da) 2001-03-26
KR19990071817A (ko) 1999-09-27
CZ194598A3 (cs) 1998-11-11
HUP9903742A3 (en) 2000-10-30
CA2234118C (en) 2009-04-07
EP0877797A1 (en) 1998-11-18
CN1154717C (zh) 2004-06-23
SK83598A3 (en) 1999-01-11
WO1997023602A1 (en) 1997-07-03
CZ297289B6 (cs) 2006-10-11
BR9612256A (pt) 1999-07-13
ATE199390T1 (de) 2001-03-15
AU730222B2 (en) 2001-03-01
JP2000506374A (ja) 2000-05-30
DE69611894D1 (de) 2001-04-05
NO326190B1 (no) 2008-10-13
IL124191A (en) 2004-06-01

Similar Documents

Publication Publication Date Title
GR3035719T3 (en) Immortalized human skin cell lines and serum-free medium for the production thereof
CA2157391A1 (en) Human liver epithelial cell lines
AU6365498A (en) Method of producing hydrogen using solid electrolyte membrane
AU3752795A (en) Methods of obtaining compositions enriched for hematopoietic stem cells, compositions derived therefrom and methods of use thereof
IL130281A0 (en) Serum-free cell culture media
EP0960124A4 (en) SERINE SUBSTITUTED MUTANTS OF CELL DEATH REGULATOR ASSOCIATED WITH BCL-XL / BCL-2
AU6039696A (en) Myogenic differentiation of human mesenchymal stem cells
EP0682107A3 (en) Process for the long-term culture of cells of the dermal papilla.
IL131740A (en) Method for the propagation of hair
CA2222425A1 (en) Immortalization and disimmortalization of cells
AU6855796A (en) In vivo electroporation of cells
HUP9802640A3 (en) Cell culture method organ tissue implantum from cell population which was made by this method
IL83564A (en) Enzymatic membrane method for the synthesis and separation of l,l-di-peptides
AU7241791A (en) Electrochemical synthesis of hydrogen peroxide
IL146228A0 (en) A method of screening compounds
AU7739296A (en) Novel method of culturing human epithelial cells for the identification of cancer therapeutics and diagnostics
GB9704772D0 (en) Method of producing hydrogen from biomass
DK0858502T3 (da) Normale, neurale epitelprecursorceller
GB9605453D0 (en) Cultured cells
IL158526A0 (en) Method and composition for skin grafts
AU8067598A (en) Membrane and method for synthesis of hydrogen peroxide
AU4264597A (en) Cultured cell-seeded fibrous lattice for tissue replacement
MX9709783A (es) Inmortalizacion y desinmortalizacion de celulas.
GB9513428D0 (en) Culturing of cells
AUPO275296A0 (en) Method for engraftment of cultured cells