BR9612256B1 - método aperfeiçoado para a imortalização de células cutáneas humanas e meio de cultura. - Google Patents

método aperfeiçoado para a imortalização de células cutáneas humanas e meio de cultura.

Info

Publication number: BR9612256B1
Authority: BR; Brazil
Prior art keywords: human skin; immortalization; culture medium; improved method; skin cells
Prior art date: 1995-12-21

Application number

BRPI9612256-0A

Other languages

English (en)

Other versions

BR9612256A (pt

Inventor

Markus Baur

Catherine Mace

Armand Malnoe

Andrea M A Pfeifer

Marcelle Rignier

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1995-12-21

Filing date

1996-12-19

Publication date

2010-08-10

1996-12-19 Application filed filed Critical

1999-07-13 Publication of BR9612256A publication Critical patent/BR9612256A/pt

2010-08-10 Publication of BR9612256B1 publication Critical patent/BR9612256B1/pt

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0625—Epidermal cells, skin cells; Cells of the oral mucosa
- C12N5/0629—Keratinocytes; Whole skin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/05—Inorganic components
- C12N2500/10—Metals; Metal chelators
- C12N2500/20—Transition metals
- C12N2500/24—Iron; Fe chelators; Transferrin
- C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/32—Amino acids
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/38—Vitamins
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/40—Nucleotides, nucleosides or bases
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/44—Thiols, e.g. mercaptoethanol
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/30—Organic components
- C12N2500/46—Amines, e.g. putrescine
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/11—Epidermal growth factor [EGF]
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/10—Growth factors
- C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/30—Hormones
- C12N2501/38—Hormones with nuclear receptors
- C12N2501/39—Steroid hormones
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/80—Neurotransmitters; Neurohormones
- C12N2501/81—Adrenaline
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/999—Small molecules not provided for elsewhere
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/04—Immortalised cells

Landscapes

Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Life Sciences & Earth Sciences (AREA)
Biomedical Technology (AREA)
Organic Chemistry (AREA)
Chemical & Material Sciences (AREA)
Bioinformatics & Cheminformatics (AREA)
Genetics & Genomics (AREA)
Biotechnology (AREA)
Wood Science & Technology (AREA)
Zoology (AREA)
Microbiology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Cell Biology (AREA)
Dermatology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Materials For Medical Uses (AREA)
Medicines Containing Material From Animals Or Micro-Organisms (AREA)

BRPI9612256-0A 1995-12-21 1996-12-19 método aperfeiçoado para a imortalização de células cutáneas humanas e meio de cultura. BR9612256B1 (pt)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
US57648395A	1995-12-21	1995-12-21
PCT/EP1996/005812 WO1997023602A1 (en)	1995-12-21	1996-12-19	Improved immortalized human skin cell lines and novel serum-free medium useful for the production thereof

Publications (2)

Publication Number	Publication Date
BR9612256A BR9612256A (pt)	1999-07-13
BR9612256B1 true BR9612256B1 (pt)	2010-08-10

Family

ID=24304605

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
BRPI9612256-0A BR9612256B1 (pt)	1995-12-21	1996-12-19	método aperfeiçoado para a imortalização de células cutáneas humanas e meio de cultura.

Country Status (23)

Country	Link
US (2)	US6423540B2 (pt)
EP (2)	EP0877797A1 (pt)
JP (1)	JP4404965B2 (pt)
KR (1)	KR100455006B1 (pt)
CN (1)	CN1154717C (pt)
AT (1)	ATE199390T1 (pt)
AU (1)	AU730222B2 (pt)
BR (1)	BR9612256B1 (pt)
CA (1)	CA2234118C (pt)
CZ (1)	CZ297289B6 (pt)
DE (1)	DE69611894T2 (pt)
DK (1)	DK0780469T3 (pt)
ES (1)	ES2155166T3 (pt)
GR (1)	GR3035719T3 (pt)
HU (1)	HU226195B1 (pt)
IL (1)	IL124191A (pt)
NO (1)	NO326190B1 (pt)
NZ (1)	NZ325814A (pt)
PL (1)	PL195108B1 (pt)
PT (1)	PT780469E (pt)
RU (1)	RU2215030C2 (pt)
SK (1)	SK283314B6 (pt)
WO (1)	WO1997023602A1 (pt)

Families Citing this family (30)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE69611894T2 (de) *	1995-12-21	2001-09-13	Societe Des Produits Nestle S.A., Vevey	Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung
JP4671504B2 (ja) *	1998-04-17	2011-04-20	ソシエテ・デ・プロデュイ・ネスレ・エス・アー	正常ヒト皮膚組織由来の不死化した細胞系
US6964869B2 (en)	1998-07-13	2005-11-15	Wisconsin Alumni Research Foundation	Method and composition for skin grafts
CN1087778C (zh) *	1999-03-02	2002-07-17	华东理工大学	杂交瘤细胞无血清培养基
DE19912798C1 (de) *	1999-03-10	2000-02-17	Andreas Jordan	Verfahren zur Kultivierung von Krebszellen aus Humangewebe und Vorrichtung zur Aufbereitung von Gewebeproben
AUPR298901A0 (en)	2001-02-07	2001-03-08	McComb Foundation, Inc., The	Cell suspension preparation technique and device
DE60221519T2 (de) *	2001-04-24	2008-04-17	Wisconsin Allumni Research Foundation, Madison	Verfahren und zusammensetzung für hauttransplantate
US7262174B2 (en)	2001-05-09	2007-08-28	Geron Corporation	Treatment for wounds
US20030022369A1 (en) *	2001-05-18	2003-01-30	Helen Fillmore	Differentiation of specialized dermal and epidermal cells into neuronal cells
CA2452660A1 (en) *	2001-07-06	2003-01-16	Lipomics Technologies, Inc.	Generating, viewing, interpreting, and utilizing a quantitative database of metabolites
GB0208041D0 (en)	2002-04-08	2002-05-22	Lonza Biologics Plc	Method of culturing animal cells
US20050025737A1 (en) *	2003-07-30	2005-02-03	Sebagh Jean Louis	Compositions containing melon extracts
WO2005028626A2 (en) *	2003-09-18	2005-03-31	Raven Biotechnologies, Inc.	Cell culture media
WO2005071065A1 (en) *	2004-01-26	2005-08-04	Cognis France S.A.S	Method for studying functional interactions between sensory neurons and keratinocytes or melanocytes
US9295543B2 (en)	2005-03-17	2016-03-29	Stratatech Corporation	Skin substitutes with improved purity
DE602007008627D1 (de) *	2006-02-14	2010-10-07	Genetix Ltd	Zellkulturmedium
WO2010065907A2 (en) *	2008-12-05	2010-06-10	The United States Of America As Represented By The Secretary, Department Of Health And Human Services	Use of a rock inhibitor to sustain primary human keratinocytes in a proliferative state
US20120142103A1 (en) *	2009-05-18	2012-06-07	Kohji Nishida	Method for inducing differentiation into epithelial progenitor cell/stem cell population and corneal epithelial cell population from induced pluripotent stem cells
KR101768632B1 (ko) *	2011-05-18	2017-08-17	(주)아모레퍼시픽	칼슘 펄스를 통한 배양상피세포층의 분리방법
AU2012283667B2 (en) *	2011-07-12	2015-07-09	Foodchek Systems, Inc.	Culture medium, method for culturing Salmonella and E. coli and method for detecting Salmonella and E. coli
WO2013129885A1 (ko) *	2012-02-28	2013-09-06	건국대학교 산학협력단	세포 배양액
WO2014142038A1 (ja)	2013-03-11	2014-09-18	Ｊｃｒファーマ株式会社	ヒト角膜上皮シートの製造法
AU2013205148B2 (en)	2013-03-14	2014-10-30	AVITA Medical Americas, LLC	Systems and methods for tissue processing and preparation of cell suspension therefrom
WO2016085304A1 (ko) *	2014-11-28	2016-06-02	(주)아모레퍼시픽	멜라닌 세포주 계대배양계 및 이를 이용한 노화 수준 판단, 미용 정보 제공, 또는 스크리닝 방법
GB2547605A (en) *	2015-01-26	2017-08-23	Halliburton Energy Services Inc	Rotating superhard cutting element
CN105238739A (zh) *	2015-11-11	2016-01-13	朱宁文	临床治疗级细胞治疗用黑色素细胞(melanocytes)规模化制备人类细胞外基质筛选培养方法
CN106520674A (zh) *	2016-12-24	2017-03-22	严志海	一种用于表皮黑素细胞体外培养的无血清培养基
CN107115219A (zh) *	2017-05-12	2017-09-01	青岛赛欧凯普图乐生物医药有限公司	一种纤维芽母细胞活化液面膜配方
CN114058565A (zh) *	2020-07-31	2022-02-18	上海尚瑞生物医药科技有限公司	一种无血清的黑素母细胞培养液及其培养方法
KR102583179B1 (ko) *	2020-11-27	2023-09-26	(주)엑셀세라퓨틱스	칼슘, 상피세포성장인자 및 알부민을 포함하는 각질형성세포 수립 또는 배양용 배지 조성물

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US4940666A (en) *	1983-07-15	1990-07-10	University Patents, Inc.	Process and defined medium for growth of human epidermal keratinocyte cells
US4885238A (en)	1987-10-30	1989-12-05	The United States Of America As Represented By The Department Of Health And Human Services	Immortalized human bronchial epitherial mesothelial cell lines
US5292655A (en)	1990-01-29	1994-03-08	Wille Jr John J	Method for the formation of a histologically-complete skin substitute
US5376542A (en)	1992-04-27	1994-12-27	Georgetown University	Method for producing immortalized cell lines using human papilluma virus genes
AU2385795A (en) *	1994-04-12	1995-10-30	Alza Corporation	Screening methods for integumental inflammation modulating agents
DE19617261A1 (de) *	1995-04-21	1996-11-28	Naeher Helmut Dr Med Priv Doz	Hautverträglichkeitstest
DE69611894T2 (de) *	1995-12-21	2001-09-13	Societe Des Produits Nestle S.A., Vevey	Unsterbliche humane Hautzell-Linien und serumfreies Medium für ihre Herstellung

1996
- 1996-12-19 DE DE69611894T patent/DE69611894T2/de not_active Expired - Lifetime
- 1996-12-19 ES ES96203641T patent/ES2155166T3/es not_active Expired - Lifetime
- 1996-12-19 IL IL12419196A patent/IL124191A/en not_active IP Right Cessation
- 1996-12-19 SK SK835-98A patent/SK283314B6/sk unknown
- 1996-12-19 CZ CZ0194598A patent/CZ297289B6/cs not_active IP Right Cessation
- 1996-12-19 NZ NZ325814A patent/NZ325814A/xx unknown
- 1996-12-19 WO PCT/EP1996/005812 patent/WO1997023602A1/en active IP Right Grant
- 1996-12-19 EP EP96944641A patent/EP0877797A1/en not_active Withdrawn
- 1996-12-19 CN CNB961991755A patent/CN1154717C/zh not_active Expired - Fee Related
- 1996-12-19 KR KR10-1998-0704097A patent/KR100455006B1/ko not_active IP Right Cessation
- 1996-12-19 JP JP52332997A patent/JP4404965B2/ja not_active Expired - Fee Related
- 1996-12-19 BR BRPI9612256-0A patent/BR9612256B1/pt not_active IP Right Cessation
- 1996-12-19 CA CA002234118A patent/CA2234118C/en not_active Expired - Fee Related
- 1996-12-19 US US09/091,483 patent/US6423540B2/en not_active Expired - Lifetime
- 1996-12-19 RU RU98113400/13A patent/RU2215030C2/ru not_active IP Right Cessation
- 1996-12-19 PT PT96203641T patent/PT780469E/pt unknown
- 1996-12-19 AT AT96203641T patent/ATE199390T1/de active
- 1996-12-19 DK DK96203641T patent/DK0780469T3/da active
- 1996-12-19 EP EP96203641A patent/EP0780469B1/fr not_active Expired - Lifetime
- 1996-12-19 PL PL96327320A patent/PL195108B1/pl unknown
- 1996-12-19 AU AU13054/97A patent/AU730222B2/en not_active Ceased
- 1996-12-19 HU HU9903742A patent/HU226195B1/hu not_active IP Right Cessation
1998
- 1998-06-18 NO NO19982810A patent/NO326190B1/no not_active IP Right Cessation
2001
- 2001-04-06 GR GR20010400570T patent/GR3035719T3/el unknown
- 2001-10-18 US US09/982,649 patent/US6949381B2/en not_active Expired - Fee Related

Also Published As

Publication number	Publication date
PL327320A1 (en)	1998-12-07
SK283314B6 (sk)	2003-05-02
KR100455006B1 (ko)	2005-01-13
PT780469E (pt)	2001-06-29
NZ325814A (en)	2000-10-27
NO982810L (no)	1998-08-21
US6949381B2 (en)	2005-09-27
EP0780469A1 (fr)	1997-06-25
NO982810D0 (no)	1998-06-18
HUP9903742A2 (hu)	2000-03-28
HU226195B1 (hu)	2008-06-30
US20020012993A1 (en)	2002-01-31
CA2234118A1 (en)	1997-07-03
AU1305497A (en)	1997-07-17
EP0780469B1 (fr)	2001-02-28
CN1205737A (zh)	1999-01-20
RU2215030C2 (ru)	2003-10-27
US6423540B2 (en)	2002-07-23
JP4404965B2 (ja)	2010-01-27
PL195108B1 (pl)	2007-08-31
US20020042129A1 (en)	2002-04-11
ES2155166T3 (es)	2001-05-01
DE69611894T2 (de)	2001-09-13
DK0780469T3 (da)	2001-03-26
KR19990071817A (ko)	1999-09-27
CZ194598A3 (cs)	1998-11-11
HUP9903742A3 (en)	2000-10-30
CA2234118C (en)	2009-04-07
EP0877797A1 (en)	1998-11-18
CN1154717C (zh)	2004-06-23
SK83598A3 (en)	1999-01-11
WO1997023602A1 (en)	1997-07-03
CZ297289B6 (cs)	2006-10-11
BR9612256A (pt)	1999-07-13
ATE199390T1 (de)	2001-03-15
AU730222B2 (en)	2001-03-01
JP2000506374A (ja)	2000-05-30
DE69611894D1 (de)	2001-04-05
NO326190B1 (no)	2008-10-13
IL124191A (en)	2004-06-01
GR3035719T3 (en)	2001-07-31

Publication	Publication Date	Title
BR9612256B1 (pt)	2010-08-10	método aperfeiçoado para a imortalização de células cutáneas humanas e meio de cultura.
PT971679E (pt)	2002-11-29	Metodo de reproducao de cabelo
NZ288467A (en)	1998-10-28	In vitro method for culturing cartilage tissue by culturing stromal cells in the presence of growth factors
AU3752795A (en)	1996-06-06	Methods of obtaining compositions enriched for hematopoietic stem cells, compositions derived therefrom and methods of use thereof
WO1997012960A3 (en)	2001-12-20	Solid support for use in cell cultivation, especially for the cultivation of liver cells, biological reactor containing said solid support and the use thereof in a bio-artificial liver system
DE69638059D1 (de)	2009-12-03	Kulturmedium für mikroorganismen
NO20006096D0 (no)	2000-11-30	Medium for dyrking av dyreceller og fremgangsmåte for fremstilling av protein ved anvendelse av det samme
EP0682107A3 (en)	1997-04-16	Process for the long-term culture of cells of the dermal papilla.
HUP9802640A3 (en)	1999-04-28	Cell culture method organ tissue implantum from cell population which was made by this method
HUP0101913A2 (hu)	2001-09-28	Normál humán bőrszövetekből származó, immortalizált sejtvonal
ATE521696T1 (de)	2011-09-15	Verfahren zur herstellung von zelltransplantaten
CY1106955T1 (el)	2012-09-26	Μεθοδος για συνθεση για δερματικα μοσχευματα
DK0858502T3 (da)	2005-05-23	Normale, neurale epitelprecursorceller
EP0117486A3 (en)	1987-05-13	Method for growth and purification of human cell lines from heterogeneous cell populations in tissue culture
DE3785109D1 (de)	1993-05-06	Immortalisierung durch dns-uebertragung.
MX9709783A (es)	1998-03-31	Inmortalizacion y desinmortalizacion de celulas.
MX9605491A (es)	1998-02-28	Colageno a partir de cultivos celulares.

Legal Events

Date	Code	Title	Description
2005-09-20	B07A	Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
2008-02-26	B07A	Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
2009-04-22	B06A	Patent application procedure suspended [chapter 6.1 patent gazette]
2009-11-03	B09A	Decision: intention to grant [chapter 9.1 patent gazette]
2010-08-10	B16A	Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]	Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 10/08/2010, OBSERVADAS AS CONDICOES LEGAIS.
2016-10-25	B21F	Lapse acc. art. 78, item iv - on non-payment of the annual fees in time	Free format text: REFERENTE A 20A ANUIDADE.
2017-02-21	B24J	Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)
2018-03-27	B15K	Others concerning applications: alteration of classification	Ipc: C12N 5/071 (2010.01), A61K 35/12 (2015.0