CN105341530A - Clean type special compound feed for stichopus japonicus and processing method of clean type special compound feed - Google Patents
Clean type special compound feed for stichopus japonicus and processing method of clean type special compound feed Download PDFInfo
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- 238000002360 preparation method Methods 0.000 claims abstract description 15
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 14
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- 239000000758 substrate Substances 0.000 claims description 2
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 claims 1
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
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Abstract
本发明涉及一种清洁型刺参专用配合饲料及加工方法,属于水产养殖饲料加工技术领域。其由以下原料制得:海藻粉10-40份,酶解豆粕和酶解苜蓿草粉反应混合液3-80份,微晶纤维素5-15份,鱼粉3-5份,小麦粉3-5份,酵母粉2-5份,淀粉4-7份,褐藻酸钠4-8份,海泥20-30份,复合维生素0.7-2份,复合矿物质1-3份。工艺步骤:1)备料;2)粉碎;3)二次混合;4)挤压制粒;5)烘干;6)冷却和包装。本发明利用复合蛋白酶对植物蛋白原料进行生物酶解处理,再将植物蛋白酶解物和海藻粉进行适当配比,强化饲料的整体营养价值,提高饲料的易消化吸收性。制作工艺上利用营养散失动力学微粒子技术,制得无污染易清洁型刺参专用高效配合饲料。The invention relates to a clean type sea cucumber special compound feed and a processing method thereof, belonging to the technical field of aquaculture feed processing. It is prepared from the following raw materials: 10-40 parts of seaweed powder, 3-80 parts of reaction mixture of enzymatically hydrolyzed soybean meal and enzymolyzed alfalfa powder, 5-15 parts of microcrystalline cellulose, 3-5 parts of fish meal, 3-5 parts of wheat flour 2-5 parts of yeast powder, 4-7 parts of starch, 4-8 parts of sodium alginate, 20-30 parts of sea mud, 0.7-2 parts of multivitamins, and 1-3 parts of multivitamins. Process steps: 1) material preparation; 2) crushing; 3) secondary mixing; 4) extrusion granulation; 5) drying; 6) cooling and packaging. The invention uses compound protease to carry out biological enzymolysis treatment on plant protein raw materials, and then properly mixes the plant protein enzymatic hydrolyzate and seaweed powder to strengthen the overall nutritional value of the feed and improve the digestibility and absorption of the feed. In the production process, the microparticle technology of nutrient loss kinetics is used to produce a non-polluting and easy-to-clean high-efficiency compound feed for sea cucumbers.
Description
技术领域technical field
本发明涉及一种清洁型刺参专用配合饲料及加工方法,具体讲是采用营养缓释技术结合添加酶解植物蛋白生产清洁型刺参专用配合饲料,属于水产养殖饲料加工技术领域。The invention relates to a clean type sea cucumber special compound feed and a processing method thereof. Specifically, the clean type sea cucumber special compound feed is produced by adopting a nutrient slow-release technology combined with adding enzymatic hydrolyzed plant protein, and belongs to the technical field of aquaculture feed processing.
背景技术Background technique
近几年,刺参养殖开始进入集约化高密度养殖,养殖中大量消耗鼠尾藻、马尾藻、海带等海洋藻类,既造成海藻资源的缺乏,又提高了海藻的价格。虽然目前采用陆生植物蛋白替代藻粉在一定程度上缓解了对藻粉的压力,但是替代后常常导致刺参摄食率降低、生长变慢等情况,陆生植物替代藻粉只能维持在较低的比例上。究其原因,同鱼虾类相比,刺参自身的消化酶谱较为简单,刺参通过采用大量摄食来摄入较多的饵料来弥补消化吸收低的缺点,而简单的替代则导致其消化吸收能力进一步降低。另外,在刺参饲料中多采用粉状饲料,粉状料在水中散失大,尤其一些可溶性营养物质极易散失到水中,相对降低了刺参对其有效的摄取。同时,散失的营养成分常常造成池水底部严重污染,粘在池底部的配合饲料不易清理,更加重底部污染,造成刺参明显的“回避行为”,增大刺参的死亡,给刺参养殖带来损失。片状饲料虽然具有一定的抗散失性,但是与粉状饲料不同,片状饲料常沉在底部一小块区域,由于饲料配方中缺少强烈的诱食性物质,刺参的感觉器官难以在短时间内探测到饲料的位置,这样也相对增加了片状饲料在水中的滞留时间,其实质上是增加了营养物质的散失。解决这些问题的有效的方法之一,就是针对配方中的各种营养物质的散失动力学规律,设计一种含有丰富诱食物质的强稳定性配合饲料,使其在水中层层崩解,缓慢微量释放诱食物质,引诱刺参主动摄食,同时配合饲料中需具有易消化性物质,辅助提高刺参对营养物质的消化。In recent years, sea cucumber farming has begun to enter intensive high-density farming, which consumes a large amount of marine algae such as sargassum, sargassum, and kelp, which not only causes a shortage of seaweed resources, but also increases the price of seaweed. Although the current use of terrestrial plant protein to replace algal flour has relieved the pressure on algal flour to a certain extent, it often leads to lower feeding rates and slower growth of Apostichopus japonicus. low ratio. The reason is that compared with fish and shrimp, the digestive enzyme spectrum of sea cucumber itself is relatively simple. Sea cucumber uses a large amount of food to ingest more bait to make up for the shortcoming of low digestion and absorption, and simple substitution leads to its digestive enzymes. Absorptive capacity is further reduced. In addition, the japonicus japonicus feed mostly uses powdered feed, and the powdered feed loses a lot in water, especially some soluble nutrients are easily lost into the water, which relatively reduces the effective intake of japonicus japonicus. At the same time, the lost nutrients often cause serious pollution at the bottom of the pool, and the compound feed stuck to the bottom of the pool is not easy to clean up, which makes the bottom more polluted, causing the obvious "avoidance behavior" of sea cucumbers, increasing the death of sea cucumbers, and bringing harm to sea cucumber cultivation. come loss. Although the flake feed has a certain resistance to loss, it is different from the powder feed, and the flake feed often sinks in a small area at the bottom. Due to the lack of strong food-attracting substances in the feed formula, it is difficult for the sensory organs of sea cucumbers to be absorbed in a short time. The position of the feed is detected within, which relatively increases the residence time of the flake feed in the water, which in essence increases the loss of nutrients. One of the effective ways to solve these problems is to design a strong and stable compound feed rich in attractant substances according to the loss kinetics of various nutrients in the formula, so that it can disintegrate layer by layer in water and slowly A small amount of food-attracting substances is released to lure sea cucumbers to actively eat. At the same time, the compound feed needs to have digestible substances to help improve the digestion of nutrients by sea cucumbers.
发明内容Contents of the invention
本发明的目的在于解决刺参对饲料中的组成原料消化率低、摄取有效养分不足的缺点,提供一种清洁型刺参专用高效配合饲料及加工方法,以提高饲料的整体营养价值,同时结合缓释诱食技术,提高刺参主动摄食能力,促进其生长。The purpose of the present invention is to solve the disadvantages of low digestibility of raw materials of sea cucumbers in the feed and insufficient intake of effective nutrients, and provide a special high-efficiency compound feed and processing method for clean sea cucumbers to improve the overall nutritional value of the feed. Slow-release food-attraction technology improves the active feeding ability of sea cucumbers and promotes their growth.
本发明通过一种简单高效的原料处理工艺,利用复合蛋白酶对植物蛋白原料进行生物酶解处理,再将植物蛋白酶解物和海藻粉进行适当配比,从而强化饲料的整体营养价值,提高饲料的易消化吸收性。在加工方面,利用前期营养散失动力学研究建立微粒子加工工艺技术,形成一种无污染易清洁型刺参专用高效配合饲料。The present invention adopts a simple and efficient raw material treatment process, uses compound protease to carry out biological enzymolysis treatment on plant protein raw materials, and then properly mixes the plant protein enzymatic hydrolyzate and seaweed powder, thereby strengthening the overall nutritional value of the feed and improving the nutritional value of the feed. Easy to digest and absorb. In terms of processing, the microparticle processing technology was established by using the previous study on the kinetics of nutrient loss to form a non-polluting and easy-to-clean special high-efficiency compound feed for sea cucumbers.
本发明是通过以下技术方案来实现的:The present invention is achieved through the following technical solutions:
一种清洁型刺参专用配合饲料,其特别之处在于由以下原料组分制得,按各组分的重量份分别为:海藻粉10-40份,酶解豆粕和酶解苜蓿草粉反应混合液3-80份,微晶纤维素5-15份,鱼粉3-5份,小麦粉3-5份,酵母粉1-5份,淀粉4-7份,褐藻酸钠4-8份,海泥20-30份,复合维生素0.7-2份,复合矿物质1-3份。A special compound feed for clean sea cucumber, which is special in that it is made of the following raw material components, according to the weight parts of each component: 10-40 parts of seaweed powder, the reaction of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder 3-80 parts of mixed liquid, 5-15 parts of microcrystalline cellulose, 3-5 parts of fish meal, 3-5 parts of wheat flour, 1-5 parts of yeast powder, 4-7 parts of starch, 4-8 parts of sodium alginate, sea 20-30 parts of mud, 0.7-2 parts of multivitamins, and 1-3 parts of compound minerals.
所述酶解豆粕和酶解苜蓿草粉反应混合液的制作方法为:The preparation method of the reaction mixture of the enzymolysis soybean meal and the enzymolysis alfalfa powder is as follows:
1)制备复合酶反应液:1) Prepare compound enzyme reaction solution:
以豆粕和苜蓿草粉的总用量为参照底物,取碱性蛋白酶、非淀粉多糖酶和风味酶,取量分别按照与豆粕和苜蓿草粉总重量比的0.08~0.22%、0.1~0.4%、0.06~0.3%,调配混合,加水搅拌使其充分溶解后,放入带有搅拌功能和温度控制功能的容器中,升温至45~55℃;Taking the total amount of soybean meal and alfalfa powder as the reference substrate, take alkaline protease, non-starch polysaccharide enzyme and flavor enzyme, and take the amount of 0.08-0.22% and 0.1-0.4% of the total weight ratio of soybean meal and alfalfa powder respectively , 0.06-0.3%, blend and mix, add water and stir to make it fully dissolved, put it into a container with stirring function and temperature control function, and heat up to 45-55°C;
2)酶解豆粕和酶解苜蓿草粉:2) Enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder:
将豆粕和苜蓿草进行超微粉碎,过120~150目筛网,按比例加入配制好的复合酶反应液中,添加比例以豆粕粉和苜蓿草粉重量/复合酶反应液体积为1:5~1:2(w/v,g/mL),放入酶解反应罐中进行酶解反应,搅拌转速设置为30-60r/min,酶解作用时间为4-6小时,4-6小时后制成酶解豆粕和酶解苜蓿草粉反应混合液。Superfinely pulverize soybean meal and alfalfa grass, pass through a 120-150 mesh screen, and add to the prepared compound enzyme reaction solution in proportion. ~1:2 (w/v, g/mL), put it into the enzymolysis reaction tank for enzymolysis reaction, set the stirring speed at 30-60r/min, and the enzymolysis time is 4-6 hours, 4-6 hours Afterwards, a reaction mixture of enzymatically hydrolyzed soybean meal and enzymolyzed alfalfa powder is prepared.
酶解工艺中,即所述酶解豆粕和酶解苜蓿草粉反应混合液的制作方法中豆粕与苜蓿草粉的重量比要求为2:1~3:1。In the enzymatic hydrolysis process, that is, in the preparation method of the reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder, the weight ratio of soybean meal to alfalfa powder is required to be 2:1-3:1.
所述碱性蛋白酶为市售产品,要求活性≥50000U/g。The alkaline protease is a commercially available product, and the required activity is ≥50000U/g.
所述非淀粉多糖酶为市售水产专用产品,要求含木聚糖酶≥8000U/g、β-葡聚糖酶≥2000U/g、β-甘露聚糖酶≥150U/g、纤维素酶≥300U/g。The non-starch polysaccharase is a special product for commercially available aquatic products, and it is required to contain xylanase ≥ 8000U/g, β-glucanase ≥ 2000U/g, β-mannanase ≥ 150U/g, cellulase ≥ 300U/g.
所述风味酶为市售产品,要求活性≥30000U/g。The flavor enzyme is a commercially available product, and the required activity is ≥30000U/g.
优选的,所述清洁型刺参专用配合饲料,优先选用由以下原料组分制得,按各组分的重量份分别为:海藻粉30份,酶解豆粕和酶解苜蓿草粉反应混合液55份,微晶纤维素10份,鱼粉4份,小麦粉4份,酵母粉3份,淀粉5份,褐藻酸钠5份,海泥25份,复合维生素1份,复合矿物质2份;Preferably, the clean-type sea cucumber special compound feed is preferably prepared from the following raw material components, in parts by weight of each component: 30 parts of seaweed powder, reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder 55 parts, 10 parts of microcrystalline cellulose, 4 parts of fish meal, 4 parts of wheat flour, 3 parts of yeast powder, 5 parts of starch, 5 parts of sodium alginate, 25 parts of sea mud, 1 part of multi-vitamin, 2 parts of multi-mineral;
该酶解豆粕和酶解苜蓿草粉反应混合液中豆粕与苜蓿草粉重量比例为3:1,豆粕粉和苜蓿草粉重量/复合酶反应液体积为1:5(g/mL)。The weight ratio of soybean meal and alfalfa powder in the reaction mixture of enzymatically hydrolyzed soybean meal and enzymolyzed alfalfa powder is 3:1, and the weight of soybean meal and alfalfa powder/volume of compound enzyme reaction solution is 1:5 (g/mL).
上述清洁型刺参专用配合饲料的加工方法,包括以下步骤:The processing method of the above-mentioned clean type sea cucumber special compound feed comprises the following steps:
1)备料:按各组分的重量份称取各种原料;1) material preparation: take various raw materials by weight parts of each component;
2)粉碎:将步骤1)中称量好的海藻粉、微晶纤维素、鱼粉、小麦粉、酵母粉、淀粉、褐藻酸钠和海泥进行第一次混合,混合时间为60-120秒,混合后进行粗粉,粗粉细度通过筛网;然后采用超微粉碎机进行第二次粉碎,粉碎细度至120目以上,标准筛筛上物小于5%。2) Pulverization: Mix the seaweed powder, microcrystalline cellulose, fish meal, wheat flour, yeast powder, starch, sodium alginate and sea mud weighed in step 1) for the first time, and the mixing time is 60-120 seconds. Coarse powder is carried out after mixing, and the fineness of coarse powder passes sieve; then use a superfine pulverizer to pulverize for the second time, the pulverization fineness is more than 120 mesh, and the standard sieve sieve is less than 5%.
3)二次混合:将步骤1)称量好的复合维生素和复合矿物质加入二次粉碎后的物料中,再按照配比加入制好的酶解豆粕和酶解苜蓿草粉反应液,加入前必需保证酶解豆粕和酶解苜蓿草粉反应液混匀,进行第二次混合搅拌,混合时间为90-120秒。3) Secondary mixing: Add the compound vitamins and compound minerals weighed in step 1) to the material after secondary crushing, then add the prepared enzymatic soybean meal and enzymatic alfalfa powder reaction solution according to the proportion, add It is necessary to ensure that the reaction solution of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder is mixed evenly before performing the second mixing and stirring, and the mixing time is 90-120 seconds.
4)挤压制粒:制粒模板孔径为制粒长度要求为颗粒粒径的0.6-1.0倍。4) Extrusion granulation: the pore size of the granulation template is The granulation length is required to be 0.6-1.0 times the particle size.
5)烘干:烘干温度控制在60-70℃,烘干后的物料水分不得超过12%。5) Drying: The drying temperature is controlled at 60-70°C, and the moisture content of the dried material must not exceed 12%.
6)冷却机冷却和包装:将物料温度冷却至不高于室温温度8℃的范围内,进行成品包装。6) Cooler cooling and packaging: the material temperature is cooled to a range not higher than the room temperature of 8°C, and the finished product is packaged.
本发明通过一种简单高效的原料处理工艺,利用复合蛋白酶对植物蛋白原料进行生物酶解处理,再将植物蛋白酶解物和海藻粉进行适当配比,从而强化饲料的整体营养价值,提高饲料的易消化吸收性。在加工方面,利用前期营养散失动力学研究建立微粒子加工工艺技术,形成一种无污染易清洁型刺参专用高效配合饲料。其与已有技术对比具有以下特点:The present invention adopts a simple and efficient raw material treatment process, uses compound protease to carry out biological enzymolysis treatment on plant protein raw materials, and then properly mixes the plant protein enzymatic hydrolyzate and seaweed powder, thereby strengthening the overall nutritional value of the feed and improving the nutritional value of the feed. Easy to digest and absorb. In terms of processing, the microparticle processing technology was established by using the previous study on the kinetics of nutrient loss to form a non-polluting and easy-to-clean special high-efficiency compound feed for sea cucumbers. Compared with the prior art, it has the following characteristics:
①配方中合理采用了酶解豆粕和酶解苜蓿草粉,既降低了海藻粉的比例,其产生的植物多肽进入刺参体内能够很快的被吸收利用,提高了整个配方的营养价值性。①The formula uses enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder reasonably, which not only reduces the proportion of seaweed powder, but also the plant polypeptide produced by it enters the body of sea cucumber and can be quickly absorbed and utilized, which improves the nutritional value of the whole formula.
②合理利用海藻酸钠作为主要粘合剂,并采用海泥作为可溶性营养物的载体,从而使饲料产生缓释效果,层层崩解释放营养物质,起到持续诱食的效果,使刺参产生主动集中摄食效果,养成良好的摄食习惯。② Reasonably use sodium alginate as the main binder, and use sea mud as the carrier of soluble nutrients, so that the feed can produce a slow-release effect, and the layer-by-layer disintegration releases nutrients, which has the effect of continuously attracting food. Produce the effect of active and concentrated feeding, and develop good eating habits.
③所采用的微颗粒加工工艺建立在营养散失动力学的系统研究的基础上,与片状饲料相比,其崩解后覆盖面积更大,而其营养保存效果又远高于粉状料。总之,该颗粒料避免了片状和粉状的使用弊端,集易消化特性和营养保存性等优点。③ The micro-particle processing technology adopted is based on the systematic study of nutrient loss kinetics. Compared with flake feed, it covers a larger area after disintegration, and its nutrient preservation effect is much higher than that of powder feed. In short, the granular material avoids the disadvantages of flakes and powders, and has the advantages of easy digestion and nutrient preservation.
④本发明避开了对酶解反应液的冷冻干燥处理,通过科学计算酶解反应液配比,直接将酶解反应液进行配料,极大程度的降低了成本,节省了用水。④ The present invention avoids the freeze-drying treatment of the enzymolysis reaction solution, and directly mixes the enzymolysis reaction solution by scientifically calculating the proportion of the enzymolysis reaction solution, which greatly reduces the cost and saves water.
本发明饲料的适用对象为单体重量在10克以上的刺参,与传统配方制造的刺参专用饲料相比,诱食性更好,更易消化吸收,生长更快。The applicable object of the feed of the present invention is sea cucumber with a monomer weight of more than 10 grams. Compared with the special feed for sea cucumber manufactured by traditional formula, it has better food attractant, easier digestion and absorption, and faster growth.
具体实施方式detailed description
以下给出本发明的具体实施方式,用来对本发明的构成进行进一步说明。Specific embodiments of the present invention are given below to further describe the composition of the present invention.
实施例1Example 1
本实施例的清洁型刺参专用配合饲料,选用由以下原料组分制得,按各组分的重量份分别为:海藻粉30份,酶解豆粕和酶解苜蓿草粉反应混合液55份,微晶纤维素10份,鱼粉4份,小麦粉4份,酵母粉3份,淀粉5份,褐藻酸钠5份,海泥25份,复合维生素1份,复合矿物质2份。The clean-type sea cucumber special-purpose compound feed of this embodiment is selected from the following raw material components, and the parts by weight of each component are: 30 parts of seaweed powder, 55 parts of reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder , 10 parts of microcrystalline cellulose, 4 parts of fish meal, 4 parts of wheat flour, 3 parts of yeast powder, 5 parts of starch, 5 parts of sodium alginate, 25 parts of sea mud, 1 part of multivitamin, and 2 parts of multivitamin.
其中酶解豆粕和酶解苜蓿草粉反应混合液的制作方法为:Wherein the preparation method of the reaction mixture of enzymolysis soybean meal and enzymolysis alfalfa powder is:
1)制备复合酶反应液:将碱性蛋白酶(市售,活性为50000U/g)、非淀粉多糖酶(市售水产专用,含木聚糖酶8000U/g、β-葡聚糖酶2000U/g、β-甘露聚糖酶150U/g、纤维素酶300U/g)和风味酶(市售,活性30000U/g)按照与豆粕和苜蓿草粉合计重量比的0.1%,0.2%和0.2%的比例配好(根据用量应先估算豆粕和苜蓿草粉的使用量),加水搅拌使其充分溶解,放入带有搅拌功能和温度控制功能的容器中,升温至50℃。1) Preparation of compound enzyme reaction solution: Alkaline protease (commercially available, with an activity of 50000U/g), non-starch polysaccharase (commercially available for aquatic products, containing xylanase 8000U/g, β-glucanase 2000U/g) g, β-mannanase 150U/g, cellulase 300U/g) and flavor enzyme (commercially available, activity 30000U/g) according to the total weight ratio of soybean meal and alfalfa powder 0.1%, 0.2% and 0.2% The ratio is prepared (according to the amount, the amount of soybean meal and alfalfa powder should be estimated first), add water and stir to make it fully dissolved, put it into a container with stirring function and temperature control function, and heat up to 50°C.
2)酶解豆粕和酶解苜蓿草粉:将豆粕和苜蓿草(按重量比2:1)进行超微粉碎,过120~150目筛网,按照1:5(w/v,g/mL)的比例加入配制好的复合酶反应液中,在反应容器中进行酶解反应,搅拌转速设置为30r/min,酶解作用时间为6小时,6小时后制成酶解豆粕和酶解苜蓿草粉反应混合液。2) Enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder: ultrafinely pulverize soybean meal and alfalfa (2:1 by weight), pass through a 120-150 mesh screen, and mix according to the ratio of 1:5 (w/v, g/mL) ) ratio into the prepared compound enzyme reaction liquid, carry out the enzymolysis reaction in the reaction vessel, the stirring speed is set to 30r/min, the enzymolysis action time is 6 hours, and after 6 hours, enzymolysis soybean meal and enzymolysis alfalfa are made Grass powder reaction mixture.
本实施例中清洁型刺参专用配合饲料的加工方法:The processing method of clean type sea cucumber special compound feed in the present embodiment:
1)备料:按各组分的重量份称取各种原料;1) material preparation: take various raw materials by weight parts of each component;
2)粉碎:将步骤1)中称量好的海藻粉、微晶纤维素、鱼粉、小麦粉、酵母粉、淀粉、褐藻酸钠和海泥进行第一次混合,混合时间为60秒,混合后进行粗粉,粗粉细度通过筛网;然后采用超微粉碎机进行第二次粉碎,粉碎细度至120目以上,标准筛筛上物小于5%。2) Pulverization: Mix the seaweed powder, microcrystalline cellulose, fish meal, wheat flour, yeast powder, starch, sodium alginate and sea mud weighed in step 1) for the first time, and the mixing time is 60 seconds. Coarse powder, coarse powder fineness through sieve; then use a superfine pulverizer to pulverize for the second time, the pulverization fineness is more than 120 mesh, and the standard sieve sieve is less than 5%.
3)二次混合:将步骤1)称量好的复合维生素和复合矿物质加入二次粉碎后的物料中,再按照配比加入制好的酶解豆粕和酶解苜蓿草粉反应混合液,加入前必需保证酶解豆粕和酶解苜蓿草粉反应混合液混匀,进行第二次混合搅拌,混合时间为100秒。3) Secondary mixing: Add the compound vitamins and compound minerals weighed in step 1) to the material after secondary crushing, and then add the prepared enzymatic soybean meal and enzymatic alfalfa powder reaction mixture according to the proportion, Before adding, it is necessary to ensure that the reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder is mixed well, and the second mixing and stirring is carried out, and the mixing time is 100 seconds.
4)挤压制粒:制粒模板孔径为制粒长度要求为颗粒粒径的0.8倍。4) Extrusion granulation: the pore size of the granulation template is The granulation length is required to be 0.8 times the particle size.
5)烘干:烘干温度控制在60℃,烘干后的物料水分不得超过12%。5) Drying: The drying temperature is controlled at 60°C, and the moisture content of the dried material shall not exceed 12%.
6)冷却机冷却和包装:将物料温度冷却至不高于室温温度8℃的范围内,进行成品包装。6) Cooler cooling and packaging: the material temperature is cooled to a range not higher than the room temperature of 8°C, and the finished product is packaged.
实施例2Example 2
本实施例的清洁型刺参专用配合饲料,采用以下原料组分制得,按各组分的重量份分别为:海藻粉20份,酶解豆粕和酶解苜蓿草粉反应混合液50份,微晶纤维素15份,鱼粉5份,小麦粉3份,酵母粉2份,淀粉4份,褐藻酸钠6份,海泥28.3份,复合维生素0.7份,复合矿物质1份。The clean-type sea cucumber special-purpose compound feed of the present embodiment is prepared by using the following raw material components, and the parts by weight of each component are: 20 parts of seaweed powder, 50 parts of reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder, 15 parts of microcrystalline cellulose, 5 parts of fish meal, 3 parts of wheat flour, 2 parts of yeast powder, 4 parts of starch, 6 parts of sodium alginate, 28.3 parts of sea mud, 0.7 parts of multivitamins, and 1 part of multivitamins.
其中酶解豆粕和酶解苜蓿草粉反应混合液的制作方法为:Wherein the preparation method of the reaction mixture of enzymolysis soybean meal and enzymolysis alfalfa powder is:
1)制备复合酶反应液:将碱性蛋白酶(市售,活性80000U/g)、非淀粉多糖酶(市售水产专用,含木聚糖酶10000U/g、β-葡聚糖酶4000U/g、β-甘露聚糖酶180U/g、纤维素酶500U/g)和风味酶(市售,活性50000U/g)分别按照与豆粕和苜蓿草粉合计重量比为0.15%,0.08%和0.1%的比例配好,加水搅拌使其充分溶解,放入带有搅拌功能和温度控制功能的容器中,升温至55℃;1) Preparation of compound enzyme reaction liquid: Alkaline protease (commercially available, activity 80000U/g), non-starch polysaccharase (commercially available for aquatic products, containing xylanase 10000U/g, β-glucanase 4000U/g , β-mannanase 180U/g, cellulase 500U/g) and flavor enzyme (commercially available, activity 50000U/g) are 0.15%, 0.08% and 0.1% according to the total weight ratio of soybean meal and alfalfa powder The ratio is prepared, add water and stir to make it fully dissolved, put it into a container with stirring function and temperature control function, and heat up to 55°C;
2)酶解豆粕和酶解苜蓿草粉:将豆粕和苜蓿草按重量比例为3:1混合进行超微粉碎,过120目筛网,按照3:10(w/v,g/mL)的比例加入配制好的复合酶反应液,在反应容器中进行酶解反应,搅拌转速设置为30r/min,酶解作用时间为5小时,5小时后制成酶解豆粕和酶解苜蓿草粉反应混合液。2) Enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder: Mix soybean meal and alfalfa in a weight ratio of 3:1 for ultra-fine grinding, pass through a 120-mesh sieve, and mix according to the ratio of 3:10 (w/v, g/mL) Proportionally add the prepared compound enzyme reaction liquid, carry out enzymolysis reaction in the reaction container, set the stirring speed at 30r/min, and enzymolysis time is 5 hours, after 5 hours, make enzymolysis soybean meal and enzymolysis alfalfa powder reaction Mixture.
本实施例中清洁型刺参专用配合饲料的加工方法:The processing method of clean type sea cucumber special compound feed in the present embodiment:
1)备料:按各组分的重量份称取各种原料;1) material preparation: take various raw materials by weight parts of each component;
2)粉碎:将步骤1)中称量好的海藻粉、微晶纤维素、鱼粉、小麦粉、酵母粉、淀粉、褐藻酸钠和海泥进行第一次混合,混合时间为120秒,混合后进行粗粉,粗粉细度通过筛网;然后采用超微粉碎机进行第二次粉碎,粉碎细度至120目以上,标准筛筛上物小于5%。2) Pulverization: Mix the seaweed powder, microcrystalline cellulose, fish meal, wheat flour, yeast powder, starch, sodium alginate and sea mud weighed in step 1) for the first time, and the mixing time is 120 seconds. Coarse powder, coarse powder fineness through sieve; then use a superfine pulverizer to pulverize for the second time, the pulverization fineness is more than 120 mesh, and the standard sieve sieve is less than 5%.
3)二次混合:将步骤1)称量好的复合维生素和复合矿物质加入二次粉碎后的物料中,再按照配比加入制好的酶解豆粕和酶解苜蓿草粉反应混合液,加入前必需保证酶解豆粕和酶解苜蓿草粉反应混合液混匀,进行第二次混合搅拌,混合时间为120秒。3) Secondary mixing: Add the compound vitamins and compound minerals weighed in step 1) to the material after secondary crushing, and then add the prepared enzymatic soybean meal and enzymatic alfalfa powder reaction mixture according to the proportion, Before adding, it is necessary to ensure that the reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder is mixed well, and the second mixing and stirring is carried out, and the mixing time is 120 seconds.
4)挤压制粒:制粒模板孔径为制粒长度要求为颗粒粒径的0.8倍。4) Extrusion granulation: the pore size of the granulation template is The granulation length is required to be 0.8 times the particle size.
5)烘干:烘干温度控制在65℃,烘干后的物料水分不得超过12%。5) Drying: The drying temperature is controlled at 65°C, and the moisture content of the dried material must not exceed 12%.
6)冷却机冷却和包装:将物料温度冷却至不高于室温温度8℃的范围内,进行成品包装。6) Cooler cooling and packaging: the material temperature is cooled to a range not higher than the room temperature of 8°C, and the finished product is packaged.
实施例3Example 3
本实施例的清洁型刺参专用配合饲料,由以下原料组分制得,按各组分的重量份分别为:海藻粉40份,酶解豆粕和酶解苜蓿草粉反应混合液42.5份,微晶纤维素5份,鱼粉3份,小麦粉3份,酵母粉2份,淀粉4份,褐藻酸钠4份,海泥20份,复合维生素1份,复合矿物质1份。The clean-type sea cucumber special-purpose compound feed of the present embodiment is made from the following raw material components, and the parts by weight of each component are respectively: 40 parts of seaweed powder, 42.5 parts of reaction mixture of enzymatically hydrolyzed soybean meal and enzymolyzed alfalfa grass powder, 5 parts of microcrystalline cellulose, 3 parts of fish meal, 3 parts of wheat flour, 2 parts of yeast powder, 4 parts of starch, 4 parts of sodium alginate, 20 parts of sea mud, 1 part of multivitamin, and 1 part of compound mineral.
其中酶解豆粕和酶解苜蓿草粉反应混合液的制作方法为:Wherein the preparation method of the reaction mixture of enzymolysis soybean meal and enzymolysis alfalfa powder is:
1)制备复合酶反应液:将碱性蛋白酶、非淀粉多糖酶和风味酶分别按照与豆粕和苜蓿草粉的重量比0.1%,0.2%和0.2%的比例配好,加水搅拌使其充分溶解,放入带有搅拌功能和温度控制功能的容器中,升温至50℃。其中碱性蛋白酶60000U/g;非淀粉多糖酶含木聚糖酶10000U/g、β-葡聚糖酶2000U/g、β-甘露聚糖酶200U/g、纤维素酶300U/g;风味酶30000U/g。1) Preparation of compound enzyme reaction solution: prepare alkaline protease, non-starch polysaccharide enzyme and flavor enzyme according to the weight ratio of 0.1%, 0.2% and 0.2% of soybean meal and alfalfa powder respectively, add water and stir to fully dissolve , put into a container with stirring function and temperature control function, and heat up to 50°C. Among them, alkaline protease 60000U/g; non-starch polysaccharide enzyme containing xylanase 10000U/g, β-glucanase 2000U/g, β-mannanase 200U/g, cellulase 300U/g; flavor enzyme 30000U/g.
2)酶解豆粕和酶解苜蓿草粉:将豆粕和苜蓿草(按重量比2:1)进行超微粉碎,过150目筛网,按照2:5(w/v,g/mL)的比例加入配制好的复合酶反应液中,在反应容器中进行酶解反应,搅拌转速设置为40r/min,酶解作用时间为6小时,6小时后制成酶解豆粕和酶解苜蓿草粉反应混合液。2) Enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder: Superfinely pulverize soybean meal and alfalfa (2:1 by weight), pass through a 150-mesh sieve, and follow the formula of 2:5 (w/v, g/mL) Add the ratio to the prepared compound enzyme reaction liquid, carry out the enzymolysis reaction in the reaction container, set the stirring speed at 40r/min, and the enzymolysis time is 6 hours. After 6 hours, enzymolysis soybean meal and enzymolysis alfalfa powder are made reaction mixture.
本实施例中清洁型刺参专用配合饲料的加工方法:The processing method of clean type sea cucumber special compound feed in the present embodiment:
1)备料:按各组分的重量份称取各种原料;1) material preparation: take various raw materials by weight parts of each component;
2)粉碎:将步骤1)中称量好的海藻粉、微晶纤维素、鱼粉、小麦粉、酵母粉、淀粉、褐藻酸钠和海泥进行第一次混合,混合时间为120秒,混合后进行粗粉,粗粉细度通过筛网;然后采用超微粉碎机进行第二次粉碎,粉碎细度至120目以上,标准筛筛上物小于5%。2) Pulverization: Mix the seaweed powder, microcrystalline cellulose, fish meal, wheat flour, yeast powder, starch, sodium alginate and sea mud weighed in step 1) for the first time, and the mixing time is 120 seconds. Coarse powder, coarse powder fineness through sieve; then use a superfine pulverizer to pulverize for the second time, the pulverization fineness is more than 120 mesh, and the standard sieve sieve is less than 5%.
3)二次混合:将步骤1)称量好的复合维生素和复合矿物质加入二次粉碎后的物料中,再按照配比加入制好的酶解豆粕和酶解苜蓿草粉反应混合液,加入前必需保证酶解豆粕和酶解苜蓿草粉反应混合液混匀,进行第二次混合搅拌,混合时间为110秒。3) Secondary mixing: Add the compound vitamins and compound minerals weighed in step 1) to the material after secondary crushing, and then add the prepared enzymatic soybean meal and enzymatic alfalfa powder reaction mixture according to the proportion, Before adding, it is necessary to ensure that the reaction mixture of enzymatically hydrolyzed soybean meal and enzymatically hydrolyzed alfalfa powder is mixed well, and the second mixing and stirring is performed, and the mixing time is 110 seconds.
4)挤压制粒:制粒模板孔径为制粒长度要求与颗粒粒径相同。4) Extrusion granulation: the pore size of the granulation template is The granulation length is required to be the same as the granule size.
5)烘干:烘干温度控制在65℃,烘干后的物料水分不得超过12%。5) Drying: The drying temperature is controlled at 65°C, and the moisture content of the dried material must not exceed 12%.
6)冷却机冷却和包装:将物料温度冷却至不高于室温温度8℃的范围内,进行成品包装。6) Cooler cooling and packaging: the material temperature is cooled to a range not higher than the room temperature of 8°C, and the finished product is packaged.
为表明本发明的实际效果,特提供采用本发明清洁型刺参配合饲料养殖刺参的具体实例:In order to show the actual effect of the present invention, a specific example of cultivating sea cucumbers with clean type sea cucumber compound feed of the present invention is provided:
1.2014年5月3日,某养殖基地从山东日照购买单体重20克左右的刺参480多头,采用闭路循环水养殖,分为2个组,每组3个池子,两个组分别全程使用一种商品粉状饲料和本发明的一种饲料,至2014年7月2日,期间2个月时间里,饲料对刺参诱食性极好,摄食行为规律,刺参生长迅速,粪便量多且粪便成形。饲喂本发明饲料的海参增重率达到100-120%,成活率93%左右,而饲喂全藻粉饲料组的海参增重率仅为50-70%,成活率80%左右,添加一定量的酶解植物蛋白的饲料对刺参的养殖效果超过了全藻粉饲料。1. On May 3, 2014, a breeding base purchased more than 480 sea cucumbers with a single weight of about 20 grams from Rizhao, Shandong, and adopted closed-circuit circulating aquaculture. They were divided into 2 groups, each with 3 pools, and each group used a A kind of commercial powdered feed and a kind of feed of the present invention, to July 2, 2014, during 2 months time, feed is excellent to the food-attracting property of japonicus japonicus, feeding behavior is regular, japonicus japonicus grows rapidly, feces amount is much and Stool forms. The weight gain rate of sea cucumbers fed with the feed of the present invention reaches 100-120%, and the survival rate is about 93%, while the weight gain rate of sea cucumbers fed with the whole algal powder feed group is only 50-70%, and the survival rate is about 80%. A large amount of enzymatic vegetable protein feed has a better breeding effect on sea cucumbers than the whole algae powder feed.
通过实验对比发现,全藻粉状饲料底部几乎看不到刺参爬过痕迹,粪便较少,说明刺参摄食较差,且粘底严重,容易腐烂臭底,难以清洁。而本发明的饲料相对于常见的粉状商品料,具有清洁不污染水质、不粘底,诱食性好、促进集中摄食,提高生长速度的优点,能够实现刺参的低碳、环保、无害化养殖。Through experimental comparison, it is found that there are almost no traces of sea cucumber crawling on the bottom of the whole algae powder feed, and there is less feces, which shows that sea cucumbers eat poorly, and the bottom is seriously sticky, easy to rot and smelly, and difficult to clean. Compared with common powdery commercial materials, the feed of the present invention has the advantages of cleaning, not polluting water quality, not sticking to the bottom, good food attractant, promoting concentrated feeding, and improving growth rate, and can realize the low-carbon, environmental protection and harmless of sea cucumbers. chemical farming.
2.蓬莱某养殖场,2014年4月10日养殖单重30克刺参5万尾,利用水泥池进行养殖,其中3万尾刺参饲喂本发明配方饲料,另2万尾刺参饲喂海藻粉,放养密度、水温、换水量等条件相同,饲喂6个月,即同年10月10日,饲喂本发明饲料的刺参均增重达到190%左右,而海藻粉饲喂的刺参均增重则为150%左右,且大大减少了换水频率,无臭水现象发生,结论显示本发明饲料产品优势突出。2. A farm in Penglai, on April 10, 2014, 50,000 sea cucumbers with a single weight of 30 grams were cultured in cement ponds, of which 30,000 sea cucumbers were fed with the formula feed of the present invention, and the other 20,000 sea cucumbers were fed with Feeding seaweed powder, stocking density, water temperature, water exchange rate and other conditions are the same, feeding for 6 months, that is, on October 10 of the same year, the average weight gain of sea cucumbers fed with the feed of the present invention reached about 190%, while sea cucumbers fed with seaweed powder The average weight gain of sea cucumber is about 150%, and the frequency of water change is greatly reduced, and no smelly water occurs. The conclusion shows that the feed product of the present invention has outstanding advantages.
以上所列实施例仅为本发明较佳的实施方式,本发明的保护范围并不限于以上所给出的实施例,凡是根据本发明的思路所能实现的所有的技术方案,均属于本发明的保护范围。The above-listed embodiments are only preferred embodiments of the present invention, and the protection scope of the present invention is not limited to the above-given embodiments. All technical solutions that can be realized according to the thinking of the present invention belong to the present invention. scope of protection.
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