Nothing Special   »   [go: up one dir, main page]

HK1018796A1 - Altered therostable dna polymerases for sequencing. - Google Patents

Altered therostable dna polymerases for sequencing.

Info

Publication number
HK1018796A1
HK1018796A1 HK99103880A HK99103880A HK1018796A1 HK 1018796 A1 HK1018796 A1 HK 1018796A1 HK 99103880 A HK99103880 A HK 99103880A HK 99103880 A HK99103880 A HK 99103880A HK 1018796 A1 HK1018796 A1 HK 1018796A1
Authority
HK
Hong Kong
Prior art keywords
dna
dna polymerases
polymerases
present
sequencing
Prior art date
Application number
HK99103880A
Other languages
English (en)
Inventor
David H Gelfand
Lisa Vivian Kalman
Thomas W Myers
Fred Lawrence Reichert
Christopher Lim Sigua
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of HK1018796A1 publication Critical patent/HK1018796A1/xx

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10Transferases (2.)
    • C12N9/12Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241Nucleotidyltransferases (2.7.7)
    • C12N9/1252DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88Lyases (4.)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
HK99103880A 1997-09-11 1999-09-07 Altered therostable dna polymerases for sequencing. HK1018796A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5852597P 1997-09-11 1997-09-11

Publications (1)

Publication Number Publication Date
HK1018796A1 true HK1018796A1 (en) 2000-01-07

Family

ID=22017355

Family Applications (1)

Application Number Title Priority Date Filing Date
HK99103880A HK1018796A1 (en) 1997-09-11 1999-09-07 Altered therostable dna polymerases for sequencing.

Country Status (18)

Country Link
US (3) US6346379B1 (xx)
EP (1) EP0902035B1 (xx)
JP (1) JP4417452B2 (xx)
KR (1) KR100561122B1 (xx)
CN (1) CN1197962C (xx)
AT (1) ATE530567T1 (xx)
AU (1) AU741366B2 (xx)
BR (1) BR9803419B1 (xx)
CA (1) CA2243985C (xx)
CZ (1) CZ293217B6 (xx)
ES (1) ES2374910T3 (xx)
HK (1) HK1018796A1 (xx)
HU (1) HUP9802024A3 (xx)
IL (1) IL126100A (xx)
NO (1) NO327476B1 (xx)
PL (1) PL328525A1 (xx)
RU (1) RU2238978C2 (xx)
TW (1) TW513439B (xx)

Families Citing this family (76)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2000516468A (ja) * 1996-08-14 2000-12-12 ライフ テクノロジーズ,インコーポレイテッド 核酸増幅および配列決定のための安定な組成物
US6780591B2 (en) * 1998-05-01 2004-08-24 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US7875440B2 (en) 1998-05-01 2011-01-25 Arizona Board Of Regents Method of determining the nucleotide sequence of oligonucleotides and DNA molecules
US20040121394A1 (en) * 1998-08-19 2004-06-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US20030138834A1 (en) * 1998-08-19 2003-07-24 Dawson Elliott P. Method for determining polynucleotide sequence variations
US6818395B1 (en) * 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
US7501245B2 (en) * 1999-06-28 2009-03-10 Helicos Biosciences Corp. Methods and apparatuses for analyzing polynucleotide sequences
AU6768700A (en) * 1999-08-21 2001-03-19 Amersham Pharmacia Biotech Inc. Taq dna polymerases having an amino acid substitution at e681 and homologs thereof exhibiting improved salt tolerance
US7179590B2 (en) * 2000-04-18 2007-02-20 Roche Molecular Systems, Inc High temperature reverse transcription using mutant DNA polymerases
US6214557B1 (en) * 2000-06-06 2001-04-10 Washington University Cold sensitive mutant DNA polymerases
WO2002074988A2 (en) 2001-03-16 2002-09-26 The Chancellor, Master And Scholars Of The University Of Oxford Arrays and methods of use
US6875573B2 (en) 2001-07-06 2005-04-05 Amersham Biosciences Corp DNA polymerases having amino acid substitutions and homologs thereof
CA2475785A1 (en) * 2002-02-12 2003-08-21 Applera Corporation Polymerase compositions
DE10315640A1 (de) * 2003-04-04 2004-10-14 Ignatov, Konstantin Verfahren zur kontrollierten Freisetzung von Komponenten in eine Lösung
KR100571808B1 (ko) * 2003-04-16 2006-04-17 삼성전자주식회사 다층 박막 구조를 가진 dna 칩
WO2004094986A2 (en) * 2003-04-16 2004-11-04 Handylab, Inc. System and method for electrochemical detection of biological compounds
US20050170367A1 (en) * 2003-06-10 2005-08-04 Quake Stephen R. Fluorescently labeled nucleoside triphosphates and analogs thereof for sequencing nucleic acids
US7572581B2 (en) * 2003-06-30 2009-08-11 Roche Molecular Systems, Inc. 2′-terminator nucleotide-related methods and systems
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
US20060172408A1 (en) * 2003-12-01 2006-08-03 Quake Steven R Device for immobilizing chemical and biochemical species and methods of using same
CA2557177A1 (en) 2004-02-19 2005-09-01 Stephen Quake Methods and kits for analyzing polynucleotide sequences
US20060046258A1 (en) * 2004-02-27 2006-03-02 Lapidus Stanley N Applications of single molecule sequencing
US20050239085A1 (en) * 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US7635562B2 (en) * 2004-05-25 2009-12-22 Helicos Biosciences Corporation Methods and devices for nucleic acid sequence determination
US20070117103A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070117104A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US7476734B2 (en) * 2005-12-06 2009-01-13 Helicos Biosciences Corporation Nucleotide analogs
US20060024678A1 (en) * 2004-07-28 2006-02-02 Helicos Biosciences Corporation Use of single-stranded nucleic acid binding proteins in sequencing
US20060118754A1 (en) * 2004-12-08 2006-06-08 Lapen Daniel C Stabilizing a polyelectrolyte multilayer
US7220549B2 (en) * 2004-12-30 2007-05-22 Helicos Biosciences Corporation Stabilizing a nucleic acid for nucleic acid sequencing
US20060172328A1 (en) * 2005-01-05 2006-08-03 Buzby Philip R Methods and compositions for correcting misincorporation in a nucleic acid synthesis reaction
US7482120B2 (en) * 2005-01-28 2009-01-27 Helicos Biosciences Corporation Methods and compositions for improving fidelity in a nucleic acid synthesis reaction
US7524831B2 (en) * 2005-03-02 2009-04-28 Schering Corporation Treatments for Flaviviridae virus infection
US20060263790A1 (en) * 2005-05-20 2006-11-23 Timothy Harris Methods for improving fidelity in a nucleic acid synthesis reaction
US7666593B2 (en) 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
GB0518867D0 (en) * 2005-09-15 2005-10-26 Secretary Trade Ind Brit Microscopy tip
US20070117102A1 (en) * 2005-11-22 2007-05-24 Buzby Philip R Nucleotide analogs
US20070128610A1 (en) * 2005-12-02 2007-06-07 Buzby Philip R Sample preparation method and apparatus for nucleic acid sequencing
EP1963536B1 (en) * 2005-12-22 2016-05-04 Pacific Biosciences of California, Inc. Polymerases for nucleotide analogue incorporation
EP1963530B1 (en) * 2005-12-22 2011-07-27 Pacific Biosciences of California, Inc. Active surface coupled polymerases
CA2633520A1 (en) * 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
US8962293B2 (en) 2006-10-18 2015-02-24 Roche Molecular Systems, Inc. DNA polymerases and related methods
AU2007309504B2 (en) * 2006-10-23 2012-09-13 Pacific Biosciences Of California, Inc. Polymerase enzymes and reagents for enhanced nucleic acid sequencing
US20100081915A1 (en) * 2008-09-29 2010-04-01 Searete Llc, Alimited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081924A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081916A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware. Histological facilitation systems and methods
US20100081928A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological Facilitation systems and methods
US20100081927A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
US20100081926A1 (en) * 2008-09-29 2010-04-01 Searete Llc, A Limited Liability Corporation Of The State Of Delaware Histological facilitation systems and methods
FR2940291B1 (fr) 2008-12-23 2012-12-21 Isp Investments Inc Peptide derive d'hmg-coa reductase et composition cosmetique ou pharmaceutique le contenant
FR2940125B1 (fr) * 2008-12-23 2013-03-22 Isp Investments Inc Composition cosmetique ou pharmaceutique apaisante comprenant un peptide activateur de la hmg-coa reductase
FR2944445B1 (fr) 2009-04-15 2013-08-16 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique apaisant
US8933036B2 (en) 2009-04-15 2015-01-13 Isp Investments Inc. Cosmetic and/or pharmaceutical composition comprising a yeast peptide hydrolysate and use of the yeast peptide hydrolysate as an active agent for strengthening hair
FR2944526B1 (fr) 2009-04-15 2013-05-10 Isp Investments Inc Composition cosmetique et/ou pharmaceutique comprenant un hydrolysat peptidique capable de renforcer la fonction barriere
JP5876478B2 (ja) 2010-06-18 2016-03-02 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別能を有するdnaポリメラーゼ
JP5841136B2 (ja) 2010-06-18 2016-01-13 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 増大した3’末端ミスマッチ識別能を有するdnaポリメラーゼ
CA2801998C (en) 2010-06-18 2016-11-08 F. Hoffmann La-Roche Ag Dna polymerases with increased 3'-mismatch discrimination
WO2011157436A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
ES2536252T3 (es) 2010-06-18 2015-05-21 F. Hoffmann-La Roche Ag ADN polimerasas con diferenciación de desapareamientos 3' aumentada
US8759062B2 (en) 2010-06-18 2014-06-24 Roche Molecular Systems, Inc. DNA polymerases with increased 3′- mismatch discrimination
EP2582805B1 (en) 2010-06-18 2015-02-25 Roche Diagnostics GmbH Dna polymerases with increased 3'-mismatch discrimination
WO2011157432A1 (en) 2010-06-18 2011-12-22 Roche Diagnostics Gmbh Dna polymerases with increased 3'-mismatch discrimination
US8765435B2 (en) 2011-02-15 2014-07-01 Roche Molecular Systems, Inc. DNA polymerases with increased 3′-mismatch discrimination
CA2831180C (en) 2011-04-11 2017-02-14 Keith Bauer Dna polymerases with improved activity
EP2737061B1 (en) 2011-07-28 2015-09-16 Roche Diagnostics GmbH Dna polymerases with improved activity
JP6140182B2 (ja) 2011-12-08 2017-05-31 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 改善された活性を有するdnaポリメラーゼ
WO2013083264A1 (en) 2011-12-08 2013-06-13 Roche Diagnostics Gmbh Dna polymerases with improved activity
CN103998603B (zh) 2011-12-08 2016-05-25 霍夫曼-拉罗奇有限公司 具有改进活性的dna聚合酶
CN105659091A (zh) 2013-08-19 2016-06-08 卓异生物公司 用于单分子检测的测定及其应用
WO2016077795A1 (en) 2014-11-14 2016-05-19 Illumina, Inc. Polymerases
CN107257854B (zh) 2015-02-02 2022-02-15 豪夫迈·罗氏有限公司 聚合酶变体
CN113528623A (zh) 2015-02-18 2021-10-22 卓异生物公司 用于单分子检测的测定及其应用
US10526588B2 (en) 2015-05-14 2020-01-07 Roche Sequencing Solutions, Inc. Polymerase variants and uses thereof
CN108473970B (zh) * 2015-11-27 2022-09-13 国立大学法人九州大学 Dna聚合酶变体
CN112703248A (zh) 2018-09-13 2021-04-23 豪夫迈·罗氏有限公司 具有改良链置换能力的突变型dna聚合酶
US20240287481A1 (en) * 2021-03-19 2024-08-29 Mgi Tech Co., Ltd. Polymerases for efficient incorporation of nucleotides with 3'-phosphate and other 3'-terminators

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4889818A (en) 1986-08-22 1989-12-26 Cetus Corporation Purified thermostable enzyme
US5466591A (en) 1986-08-22 1995-11-14 Hoffmann-La Roche Inc. 5' to 3' exonuclease mutations of thermostable DNA polymerases
CA2018273C (en) * 1989-06-09 1999-04-06 Peter D. Senter Thermally stable cytosine deaminase
US5338678A (en) 1989-06-09 1994-08-16 Oncogen, A Limited Partnership Expression of DNA sequences encoding a thermally stable cytosine deaminase from saccharomyces
US5292658A (en) * 1989-12-29 1994-03-08 University Of Georgia Research Foundation, Inc. Boyd Graduate Studies Research Center Cloning and expressions of Renilla luciferase
US6083686A (en) * 1990-10-26 2000-07-04 Johnson & Johnson Clinical Diagnostic Systems, Inc. Increased production of Thermus aquaticus DNA polymerase in E. coli
US5446591A (en) * 1993-02-08 1995-08-29 Lockheed Missiles & Space Co., Inc. Lens mounting for use with liquid lens elements
US5571706A (en) * 1994-06-17 1996-11-05 The United States Of America As Represented By The Secretary Of Agriculture Plant virus resistance gene and methods
US6015668A (en) * 1994-09-30 2000-01-18 Life Technologies, Inc. Cloned DNA polymerases from thermotoga and mutants thereof
DK0655506T3 (da) * 1994-10-17 1997-03-10 Harvard College DNA-polymerase med modificeret nukleotidbindingssted for DNA-sekvensanalyse
US5614365A (en) 1994-10-17 1997-03-25 President & Fellow Of Harvard College DNA polymerase having modified nucleotide binding site for DNA sequencing
US5852191A (en) * 1995-06-07 1998-12-22 Carnegie Mellon University Rigidized monomethine cyanines
US6107029A (en) * 1996-07-31 2000-08-22 Message Pharmaceticals, Inc. Universal method for detecting interactions between RNA molecules and RNA binding proteins
CZ293215B6 (cs) * 1996-08-06 2004-03-17 F. Hoffmann-La Roche Ag Rekombinantní tepelně stálá DNA polymeráza, způsob její přípravy a prostředek, který ji obsahuje
US5723298A (en) * 1996-09-16 1998-03-03 Li-Cor, Inc. Cycle labeling and sequencing with thermostable polymerases
DE69841023D1 (de) * 1997-03-12 2009-09-10 Applied Biosystems Llc DNA Polymerasen mit verbesserten Fähigkeiten zum Einbau von markierten Nukleotiden

Also Published As

Publication number Publication date
PL328525A1 (en) 1999-03-15
US6346379B1 (en) 2002-02-12
ES2374910T3 (es) 2012-02-23
CA2243985C (en) 2004-08-10
JPH11137284A (ja) 1999-05-25
CA2243985A1 (en) 1999-03-11
US20030152988A1 (en) 2003-08-14
IL126100A0 (en) 1999-05-09
HUP9802024A2 (hu) 1999-05-28
ATE530567T1 (de) 2011-11-15
HU9802024D0 (en) 1998-12-28
NO984157L (no) 1999-03-12
RU2238978C2 (ru) 2004-10-27
JP4417452B2 (ja) 2010-02-17
US20020142333A1 (en) 2002-10-03
KR19990029676A (ko) 1999-04-26
BR9803419B1 (pt) 2010-09-21
CZ287498A3 (cs) 1999-03-17
CN1218832A (zh) 1999-06-09
EP0902035B1 (en) 2011-10-26
BR9803419A (pt) 2000-02-08
US7598032B2 (en) 2009-10-06
HUP9802024A3 (en) 2001-02-28
KR100561122B1 (ko) 2006-07-25
EP0902035A2 (en) 1999-03-17
CN1197962C (zh) 2005-04-20
AU8416198A (en) 1999-03-25
NO984157D0 (no) 1998-09-10
NO327476B1 (no) 2009-07-13
TW513439B (en) 2002-12-11
CZ293217B6 (cs) 2004-03-17
IL126100A (en) 2005-12-18
EP0902035A3 (en) 1999-06-23
AU741366B2 (en) 2001-11-29

Similar Documents

Publication Publication Date Title
HK1018796A1 (en) Altered therostable dna polymerases for sequencing.
MX9705961A (es) Polimerasa de adn termoestable, modificada.
AU2002321094A1 (en) A novel group of alpha-amylases and a method for identification and production of novel alpha-amylases
EP2202312A3 (en) DNA polymerases having improved labelled nucleotide incorporation properties
AU691374B2 (en) 5' to 3' exonuclease mutations of thermostable DNA polymerases
EP1034302A4 (en) DE NOVO OR "UNIVERSAL" SEQUENCING ARRAY
KR900018376A (ko) 리보핵산(rna)의 합성방법
AU3062989A (en) Purified thermostable enzyme
CA2255531A1 (en) Labeled primer for use in and detection of target nucleic acids
BR9909012A (pt) Enzima para oxidação de fenol purificada, polinucleotìdeo isolado, vetor de expressão, célula hospedeira, processos para produzir uma enzima para oxidação de fenol e para produzir uma célula hospedeira, cultura substancialmente pura, e, composição de enzima
WO1998045452A3 (en) Enzyme derived from thermophilic organisms that functions as a chromosomal replicase, and preparation and uses thereof
AU2002215010A1 (en) Thermostable polymerase based on thermococcus pacificus
WO1999036429A3 (en) Nucleobase oligomers
WO1999049010A3 (en) Phenol oxidizing enzymes and their use
Patrushev et al. The effect of various templates and oligonucleotide primers on RNA and Poly (A) synthesis by E. coli and T7 RNA polymerases
CA2089495A1 (en) Purified thermostable nucleic acid polymerase enzyme from thermotoga maritima
WO1995014774A3 (fr) Gene de resistance a la toxicite de la melasse, produits relies et leur utilisation pour analyser et ameliorer les microorganismes et les melasses

Legal Events

Date Code Title Description
PE Patent expired

Effective date: 20180910