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BR9803419A - Polimerase de dna termoestável alterada para sequenciamento. - Google Patents

Polimerase de dna termoestável alterada para sequenciamento.

Info

Publication number
BR9803419A
BR9803419A BR9803419-7A BR9803419A BR9803419A BR 9803419 A BR9803419 A BR 9803419A BR 9803419 A BR9803419 A BR 9803419A BR 9803419 A BR9803419 A BR 9803419A
Authority
BR
Brazil
Prior art keywords
sequencing
dna
polymerases
dna polymerases
thermostable
Prior art date
Application number
BR9803419-7A
Other languages
English (en)
Other versions
BR9803419B1 (pt
Inventor
David H Gelfand
Lisa Vivian Kalman
Thomas W Myers
Fred Lawrence Reichert
Christopher Lim Sigua
Original Assignee
Hoffmann La Roche
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hoffmann La Roche filed Critical Hoffmann La Roche
Publication of BR9803419A publication Critical patent/BR9803419A/pt
Publication of BR9803419B1 publication Critical patent/BR9803419B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1252—DNA-directed DNA polymerase (2.7.7.7), i.e. DNA replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/6895—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for plants, fungi or algae

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Patente de Invenção: <B>''POLIMERASES DE DNA TERMOESTáVEL ALTERADA PARA SEQuENCIAMENTO''<D>. São providas polimerases de DNA termoestáveis modificadas possuindo eficiência melhorada para incorporar nucleotídeos não-convencionais, tais como aqueles marcados com corantes da família da fluoresceína. Tais polimerases de DNA termoestável modificadas podem ser preparadas por processos da tecnologia de DNA recombinante. Polimerases de DNA termoestável recombinantes preparadas de acordo com a presente invenção são vantajosas em muitas aplicações de síntese de DNA <MU>in vitro<MV>, tais como, seq³enciamento de DNA, síntese de DNA marcado e produção de produtos de extensão de primer marcados. As ditas enzimas de polimerase são especificamente úteis nos processos de seq³enciamento de ácido nucléico de terminação em cadeia. São também providos os ácidos nucléicos codificando as polimerases de DNA termoestáveis, recombinantes da presente invenção, bem como vetores e células hospedeiras compreendendo os mesmos. Também são fornecidos cojuntos compreendendo as polimerases de DNA termoestáveis recombinantes da presente invenção. As polimerases e processos da presente invenção fornecem custo significativo e vantagens eficiente para seq³enciamento de DNA.
BRPI9803419-7A 1997-09-11 1998-09-10 polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados. BR9803419B1 (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US5852597P 1997-09-11 1997-09-11

Publications (2)

Publication Number Publication Date
BR9803419A true BR9803419A (pt) 2000-02-08
BR9803419B1 BR9803419B1 (pt) 2010-09-21

Family

ID=22017355

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9803419-7A BR9803419B1 (pt) 1997-09-11 1998-09-10 polimerase de dna termoestável recombinante e processo para produção e uso da mesma, seqüência de ácido nucléico, vetor, célula hospedeira, processo para seqüenciamento de dna, para produção de dna marcado e conjunto para seqüenciamento de dna e para produzir produtos de extensão de primer marcados.

Country Status (17)

Country Link
US (3) US6346379B1 (pt)
EP (1) EP0902035B1 (pt)
JP (1) JP4417452B2 (pt)
KR (1) KR100561122B1 (pt)
CN (1) CN1197962C (pt)
AT (1) ATE530567T1 (pt)
AU (1) AU741366B2 (pt)
BR (1) BR9803419B1 (pt)
CA (1) CA2243985C (pt)
CZ (1) CZ293217B6 (pt)
ES (1) ES2374910T3 (pt)
HU (1) HUP9802024A3 (pt)
IL (1) IL126100A (pt)
NO (1) NO327476B1 (pt)
PL (1) PL328525A1 (pt)
RU (1) RU2238978C2 (pt)
TW (1) TW513439B (pt)

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Also Published As

Publication number Publication date
CN1197962C (zh) 2005-04-20
IL126100A (en) 2005-12-18
EP0902035B1 (en) 2011-10-26
US20030152988A1 (en) 2003-08-14
RU2238978C2 (ru) 2004-10-27
CZ287498A3 (cs) 1999-03-17
KR100561122B1 (ko) 2006-07-25
BR9803419B1 (pt) 2010-09-21
NO327476B1 (no) 2009-07-13
HUP9802024A2 (hu) 1999-05-28
KR19990029676A (ko) 1999-04-26
EP0902035A3 (en) 1999-06-23
CN1218832A (zh) 1999-06-09
JPH11137284A (ja) 1999-05-25
HK1018796A1 (en) 2000-01-07
TW513439B (en) 2002-12-11
NO984157D0 (no) 1998-09-10
HU9802024D0 (en) 1998-12-28
CZ293217B6 (cs) 2004-03-17
ATE530567T1 (de) 2011-11-15
JP4417452B2 (ja) 2010-02-17
AU8416198A (en) 1999-03-25
US20020142333A1 (en) 2002-10-03
PL328525A1 (en) 1999-03-15
IL126100A0 (en) 1999-05-09
CA2243985A1 (en) 1999-03-11
US7598032B2 (en) 2009-10-06
NO984157L (no) 1999-03-12
EP0902035A2 (en) 1999-03-17
HUP9802024A3 (en) 2001-02-28
US6346379B1 (en) 2002-02-12
CA2243985C (en) 2004-08-10
ES2374910T3 (es) 2012-02-23
AU741366B2 (en) 2001-11-29

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