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ATE458047T1 - Verfahren zur verstärkung der oligonucleotid- vermittelten nucleinsäuresequenzänderung unter verwendung von zusammensetzungen mit einem hydroxyharnstoff - Google Patents

Verfahren zur verstärkung der oligonucleotid- vermittelten nucleinsäuresequenzänderung unter verwendung von zusammensetzungen mit einem hydroxyharnstoff

Info

Publication number
ATE458047T1
ATE458047T1 AT03716412T AT03716412T ATE458047T1 AT E458047 T1 ATE458047 T1 AT E458047T1 AT 03716412 T AT03716412 T AT 03716412T AT 03716412 T AT03716412 T AT 03716412T AT E458047 T1 ATE458047 T1 AT E458047T1
Authority
AT
Austria
Prior art keywords
hydroxyurea
nucleic acid
acid sequence
compositions containing
sequence change
Prior art date
Application number
AT03716412T
Other languages
English (en)
Inventor
Eric Kmiec
Hetal Parekh-Olmedo
Erin Brachman
Original Assignee
Univ Delaware
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Delaware filed Critical Univ Delaware
Application granted granted Critical
Publication of ATE458047T1 publication Critical patent/ATE458047T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00

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  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Peptides Or Proteins (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
AT03716412T 2002-03-07 2003-03-07 Verfahren zur verstärkung der oligonucleotid- vermittelten nucleinsäuresequenzänderung unter verwendung von zusammensetzungen mit einem hydroxyharnstoff ATE458047T1 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US36305302P 2002-03-07 2002-03-07
US36334102P 2002-03-07 2002-03-07
US36305402P 2002-03-07 2002-03-07
US41698302P 2002-10-07 2002-10-07
PCT/US2003/007217 WO2003075856A2 (en) 2002-03-07 2003-03-07 Methods, compositions, and kits for enhancing oligonucleotide-mediated nucleic acid sequence alteration using compositions comprising a histone deacetylase inhibitor, lambda phage beta protein, or hydroxyurea

Publications (1)

Publication Number Publication Date
ATE458047T1 true ATE458047T1 (de) 2010-03-15

Family

ID=27808811

Family Applications (1)

Application Number Title Priority Date Filing Date
AT03716412T ATE458047T1 (de) 2002-03-07 2003-03-07 Verfahren zur verstärkung der oligonucleotid- vermittelten nucleinsäuresequenzänderung unter verwendung von zusammensetzungen mit einem hydroxyharnstoff

Country Status (9)

Country Link
US (1) US7566535B2 (de)
EP (1) EP1490013B1 (de)
JP (1) JP2005518817A (de)
AT (1) ATE458047T1 (de)
AU (1) AU2003220119A1 (de)
CA (1) CA2478479A1 (de)
DE (1) DE60331297D1 (de)
IL (1) IL163875A0 (de)
WO (1) WO2003075856A2 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI319387B (en) 2002-04-05 2010-01-11 Astrazeneca Ab Benzamide derivatives
US20070072815A1 (en) * 2004-05-04 2007-03-29 Kmiec Eric B Methods and kits to increase the efficiency of oligonucleotide-directed nucleic acid sequence alteration
KR20110094268A (ko) 2008-09-11 2011-08-23 키진 엔.브이. 진단 마커 개발 방법
US8993328B2 (en) * 2008-09-22 2015-03-31 Rutgers, The State University Of New Jersey Media conditioning for improving gene delivery efficiency to differentiating embryonic stem cells
ES2964536T3 (es) 2014-05-09 2024-04-08 Univ Yale Partículas recubiertas con poliglicerol hiperramificado y métodos para su preparación
US11918695B2 (en) 2014-05-09 2024-03-05 Yale University Topical formulation of hyperbranched polymer-coated particles
EP3230445B1 (de) 2014-12-12 2024-01-24 Tod M. Woolf Zusammensetzungen und verfahren zur editierung von nukleinsäuren in zellen mit oligonukleotiden
CN107624132A (zh) * 2014-12-22 2018-01-23 苏黎士大学 膜蛋白在具有细胞壁的真核细胞中的定向进化
JP2019508037A (ja) 2016-02-16 2019-03-28 イェール ユニバーシティーＹａｌｅ Ｕｎｉｖｅｒｓｉｔ 標的化遺伝子編集を増強するための組成物およびその使用方法
US20190359973A1 (en) * 2017-01-10 2019-11-28 Christiana Care Health Services, Inc. Methods for in vitro site-directed mutagenesis using gene editing technologies
US20210024882A1 (en) * 2019-07-08 2021-01-28 Life Technologies Corporation Compositions and methods for enhancing cell culture
WO2021207673A1 (en) * 2020-04-09 2021-10-14 The Regents of the University of Colorodo, a body corporate Compositions, methods and uses for production of hematopoietic stem cells (hscs)

Family Cites Families (64)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US217377A (en) * 1879-07-08 hislop
US51270A (en) * 1865-11-28 Improvement in children s bedclothes-retainers
US143052A (en) * 1873-09-23 Improvement in flexible vertically-extensible attachments for boats
US119570A (en) * 1871-10-03 Improvement in coopers crozes and howels
US5139941A (en) * 1985-10-31 1992-08-18 University Of Florida Research Foundation, Inc. AAV transduction vectors
US5955363A (en) * 1990-01-03 1999-09-21 Promega Corporation Vector for in vitro mutagenesis and use thereof
US5432272A (en) * 1990-10-09 1995-07-11 Benner; Steven A. Method for incorporating into a DNA or RNA oligonucleotide using nucleotides bearing heterocyclic bases
US6136601A (en) * 1991-08-21 2000-10-24 Epoch Pharmaceuticals, Inc. Targeted mutagenesis in living cells using modified oligonucleotides
US5962426A (en) * 1993-06-25 1999-10-05 Yale University Triple-helix forming oligonucleotides for targeted mutagenesis
US5801154A (en) * 1993-10-18 1998-09-01 Isis Pharmaceuticals, Inc. Antisense oligonucleotide modulation of multidrug resistance-associated protein
DE733059T1 (de) * 1993-12-09 1997-08-28 Univ Jefferson Verbindungen und verfahren zur ortsspezifischen mutation in eukaryotischen zellen
US5604090A (en) * 1994-06-06 1997-02-18 Fred Hutchinson Cancer Research Center Method for increasing transduction of cells by adeno-associated virus vectors
US5780296A (en) * 1995-01-17 1998-07-14 Thomas Jefferson University Compositions and methods to promote homologous recombination in eukaryotic cells and organisms
JP3770333B2 (ja) * 1995-03-15 2006-04-26 大日本住友製薬株式会社 組換えｄｎａウイルスおよびその製造方法
US5776744A (en) * 1995-06-07 1998-07-07 Yale University Methods and compositions for effecting homologous recombination
US5912340A (en) * 1995-10-04 1999-06-15 Epoch Pharmaceuticals, Inc. Selective binding complementary oligonucleotides
US6777217B1 (en) 1996-03-26 2004-08-17 President And Fellows Of Harvard College Histone deacetylases, and uses related thereto
US6025155A (en) 1996-04-10 2000-02-15 Chromos Molecular Systems, Inc. Artificial chromosomes, uses thereof and methods for preparing artificial chromosomes
US5731181A (en) * 1996-06-17 1998-03-24 Thomas Jefferson University Chimeric mutational vectors having non-natural nucleotides
US5760012A (en) * 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations
US5888983A (en) * 1996-05-01 1999-03-30 Thomas Jefferson University Method and oligonucleobase compounds for curing diseases caused by mutations
WO1997047307A1 (en) 1996-06-14 1997-12-18 The Uab Research Foundation Use of histone deacetylase inhibitors to activate transgene expression
US5928638A (en) * 1996-06-17 1999-07-27 Systemix, Inc. Methods for gene transfer
US6057102A (en) * 1996-08-08 2000-05-02 The Aaron Diamond Aids Research Center HIV coreceptor mutants
EP0963997B1 (de) * 1996-11-18 2003-02-19 Takeshi Imanishi Neue nucleotidanaloga
US6130089A (en) * 1996-12-12 2000-10-10 Lisziewicz; Julianna Materials and methods for gene transfer
JP3756313B2 (ja) * 1997-03-07 2006-03-15 武 今西 新規ビシクロヌクレオシド及びオリゴヌクレオチド類縁体
US6043389A (en) * 1997-03-11 2000-03-28 Mor Research Applications, Ltd. Hydroxy and ether-containing oxyalkylene esters and uses thereof
US6387673B1 (en) 1997-05-01 2002-05-14 The Salk Institute For Biological Studies Compounds useful for the modulation of processes mediated by nuclear hormone receptors, methods for the identification and use of such compounds
AUPO721997A0 (en) 1997-06-06 1997-07-03 Queensland Institute Of Medical Research, The Anticancer compounds
US6821759B1 (en) * 1997-06-23 2004-11-23 The Rockefeller University Methods of performing homologous recombination based modification of nucleic acids in recombination deficient cells and use of the modified nucleic acid products thereof
EP1015469B2 (de) 1997-09-12 2015-11-18 Exiqon A/S Bi- und tri-zyklische - nukleosid, nukleotid und oligonukleotid-analoga
US6262116B1 (en) 1998-01-23 2001-07-17 Sloan-Kettering Institute For Cancer Research Transcription therapy for cancers
US6004804A (en) * 1998-05-12 1999-12-21 Kimeragen, Inc. Non-chimeric mutational vectors
US6010907A (en) 1998-05-12 2000-01-04 Kimeragen, Inc. Eukaryotic use of non-chimeric mutational vectors
WO2000021979A2 (en) 1998-10-13 2000-04-20 Fujisawa Pharmaceutical Co., Ltd. Cyclic tetrapeptide and their use as histone deacetylase inhibitor
CA2346152A1 (en) 1998-10-16 2000-04-27 Novartis Ag Promotion of self-renewal and improved gene transduction of hematopoietic stem cells by histone deacetylase inhibitors
AU1331800A (en) 1998-10-28 2000-05-15 University Of Washington Targeted gene modification by parvoviral vectors
WO2000050748A1 (en) 1999-02-24 2000-08-31 N.V. Kema Combustion unit for combusting a liquid fuel and a power generating system comprising such combustion unit
CZ20013103A3 (cs) 1999-03-02 2002-01-16 The Liposome Company, Inc. Způsob zapouzdřování bioaktivních komplexů do liposomů
JP4269041B2 (ja) 1999-03-02 2009-05-27 国立大学法人九州工業大学 新規な環状テトラペプチド誘導体とその医薬用途
JP2002537785A (ja) 1999-03-04 2002-11-12 ピーピーエル セラピューティクス （スコットランド） リミテッド 体細胞の遺伝的改変とそれらの使用
JP2002540118A (ja) 1999-03-18 2002-11-26 エクシコン エ／エス キシロ−ｌｎａ類似体
AU6718200A (en) 1999-05-03 2000-12-12 Methylgene, Inc. Inhibition of histone deacetylase
US7053207B2 (en) 1999-05-04 2006-05-30 Exiqon A/S L-ribo-LNA analogues
WO2001000788A2 (en) * 1999-06-25 2001-01-04 Northwestern University Compositions, kits, and methods for modulating survival and differentiation of multi-potential hematopoietic progenitor cells
JP2001064177A (ja) 1999-08-16 2001-03-13 Schering Ag ベンズアミド誘導体を有効成分とする製剤
US6271360B1 (en) 1999-08-27 2001-08-07 Valigen (Us), Inc. Single-stranded oligodeoxynucleotide mutational vectors
JP2001081031A (ja) 1999-08-30 2001-03-27 Schering Ag 溶解性および経口吸収性を改善したベンズアミド誘導体含有製剤
EP1212357B1 (de) 1999-09-08 2007-05-02 Sloan-Kettering Institute For Cancer Research Kristallstruktur einer deacetylase und deren inhibitoren
KR20020059393A (ko) 1999-09-08 2002-07-12 제임스 에스. 쿼크 새로운 부류의 세포분화제 및 히스톤 데아세틸라아제, 및이의 사용 방법
AR025996A1 (es) 1999-10-07 2002-12-26 Valigen Us Inc Plantas no transgenicas resistentes a los herbicidas.
CA2391129A1 (en) 1999-11-10 2001-05-17 The Uab Research Foundation Lentiviral vector transduction of hematopoietic stem cells
WO2001038322A1 (en) 1999-11-23 2001-05-31 Methylgene, Inc. Inhibitors of histone deacetylase
EP1252196B1 (de) 2000-01-05 2005-11-02 Novartis AG Hydrogele
DE60143520D1 (de) 2000-03-24 2011-01-05 Methylgene Inc Inhibitoren der histon-deacetylase
JP2003528607A (ja) 2000-03-27 2003-09-30 ユニバーシティー、オブ、デラウェア 修飾一本鎖オリゴヌクレオチドを使用した標的染色体ゲノム変換
US6936467B2 (en) 2000-03-27 2005-08-30 University Of Delaware Targeted chromosomal genomic alterations with modified single stranded oligonucleotides
EP1170008A1 (de) 2000-07-07 2002-01-09 Chemotherapeutisches Forschungsinstitut Georg-Speyer-Haus Valproinsäure und dessen Derivate als Inhibitoren der Histone deacetylase
JP4810784B2 (ja) 2000-07-17 2011-11-09 アステラス製薬株式会社 還元型ｆｋ２２８およびその用途
AU2001279069A1 (en) 2000-07-27 2002-02-13 University Of Delaware Methods for enhancing targeted gene alteration using oligonucleotides
EP1311661B1 (de) * 2000-08-14 2012-10-03 THE GOVERNMENT OF THE UNITED STATES OF AMERICA as represented by the SECRETARY OF THE DEPARTMENT OF HEALTH AND HUMAN SERVICES Erhöhte homologe rekombination vermittelt durch lambda rekombinationsproteine
WO2002026967A2 (en) 2000-09-25 2002-04-04 Thomas Jefferson University Targeted gene correction by single-stranded oligodeoxynucleotides
US7312247B2 (en) 2001-03-27 2007-12-25 Errant Gene Therapeutics, Llc Histone deacetylase inhibitors

Also Published As

Publication number Publication date
CA2478479A1 (en) 2003-09-18
WO2003075856A2 (en) 2003-09-18
IL163875A0 (en) 2005-12-18
JP2005518817A (ja) 2005-06-30
AU2003220119A1 (en) 2003-09-22
US20030207451A1 (en) 2003-11-06
US7566535B2 (en) 2009-07-28
DE60331297D1 (de) 2010-04-01
EP1490013A4 (de) 2005-12-28
EP1490013A2 (de) 2004-12-29
EP1490013B1 (de) 2010-02-17
WO2003075856A3 (en) 2004-06-24

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