FLOT2

FLOT2
Dostupne strukture
PDB	Pretraga ortologa: PDBe RCSB
Spisak PDB ID kodova
	1WIN
Identifikatori
Aliasi	FLOT2
Vanjski ID-jevi	OMIM: 131560 MGI: 103309 HomoloGene: 3293 GeneCards: FLOT2
Lokacija gena (čovjek)
Hrom.	Hromosom 17 (čovjek)
Kraj	28,897,733 bp
Lokacija gena (miš)
Hrom.	Hromosom 11 (miš)
Kraj	77,951,260 bp
Obrazac RNK ekspresije
	; ;
	Više referentnih podataka o ekspresiji
Ontologija gena
Molekularna funkcija	• protease binding; • GO:0001948, GO:0016582 vezivanje za proteine; • protein heterodimerization activity; • ionotropic glutamate receptor binding;
Ćelijska komponenta	• Vezikula; • endozom; • membrana; • cell-cell contact zone; • focal adhesion; • endocytic vesicle; • acrosomal membrane; • flotillin complex; • uropod; • basolateral plasma membrane; • apical plasma membrane; • cortical actin cytoskeleton; • perinuklearno područje citoplazme; • Kaveole; • Lipidni splav; • Egzosom; • GO:0016023 citoplazmatska vezikula; • lamellipodium; • ćelijska membrana; • dendrite cytoplasm; • sinapsa; • glutamatergic synapse; • anchored component of presynaptic active zone membrane;
Biološki proces	• positive regulation of establishment of T cell polarity; • regulation of toll-like receptor 3 signaling pathway; • regulation of heterotypic cell-cell adhesion; • protein stabilization; • negative regulation of amyloid precursor protein catabolic process; • negative regulation of gene expression; • protein localization to plasma membrane raft; • Ćelijska adhezija; • membrane raft assembly; • regulation of myoblast differentiation; • epidermis development; • protein localization to plasma membrane; • anterograde dendritic transport; • regulation of postsynaptic membrane neurotransmitter receptor levels; • positive regulation of NF-kappaB transcription factor activity;
	Izvori:Amigo / QuickGO
Ortolozi
	2319
	14252
	ENSG00000132589
	ENSMUSG00000061981
	Q14254
	Q60634
	NM_004475; NM_001330170
NM_001040403; NM_001284227; NM_001284228; NM_008028; NM_001362625;
	NM_001362626; NM_001362627
	NP_001317099; NP_004466
NP_001035493; NP_001271156; NP_001271157; NP_032054; NP_001349554;
	NP_001349555; NP_001349556
	Wikipodaci
Pogledaj/uredi – čovjek	Pogledaj/uredi – miš

Flotilin-2 je protein koji je kod ljudi kodiran genom FLOT2.^[5]^[6]

Flotilin 2 (flot-2) je visoko konzervirani protein izoliran iz domena kaveol/ lipidnog splava koji vežu receptore faktora rasta na puteve signalne transdukcije. Flot-2 se veže za PAR-1, poznati uzvodni posrednik glavnih puteva transdukcije signala uključenih u rast ćelija i metastaze, a može uticati na progresiju tumora.^[7]

Kaveole su mali domeni na unutrašnjosti ćelijske membrane, uključene u promet vezikula i transdukciju signala. Ovaj gen kodira integrirani membranski protein povezan s kaveolama, za koji se smatra da djeluje u neuronskoj signalizaciji.^[6]

Dužina polipeptidnog lanca je 428 aminokiselina, a molekulska težina 47.064 Da.^[8].

Aminokiselinska sekvenca

Simboli

C: Cistein
D: Asparaginska kiselina
E: Glutaminska kiselina
F: Fenilalanin
G: Glicin
H: Histidin
I: Izoleucin
K: Lizin
L: Leucin
M: Metionin
N: Asparagin
P: Prolin
Q: Glutamin
R: Arginin
S: Serin
T: Treonin
V: Valin
W: Triptofan
Y: Tirozin

10	20	30	40	50
MGNCHTVGPN	EALVVSGGCC	GSDYKQYVFG	GWAWAWWCIS	DTQRISLEIM
TLQPRCEDVE	TAEGVALTVT	GVAQVKIMTE	KELLAVACEQ	FLGKNVQDIK
NVVLQTLEGH	LRSILGTLTV	EQIYQDRDQF	AKLVREVAAP	DVGRMGIEIL
SFTIKDVYDK	VDYLSSLGKT	QTAVVQRDAD	IGVAEAERDA	GIREAECKKE
MLDVKFMADT	KIADSKRAFE	LQKSAFSEEV	NIKTAEAQLA	YELQGAREQQ
KIRQEEIEIE	VVQRKKQIAV	EAQEILRTDK	ELIATVRRPA	EAEAHRIQQI
AEGEKVKQVL	LAQAEAEKIR	KIGEAEAAVI	EAMGKAEAER	MKLKAEAYQK
YGDAAKMALV	LEALPQIAAK	IAAPLTKVDE	IVVLSGDNSK	VTSEVNRLLA
ELPASVHALT	GVDLSKIPLI	KKATGVQV

Reference

^ ^a ^b ^c GRCh38: Ensembl release 89: ENSG00000132589 - Ensembl, maj 2017
^ ^a ^b ^c GRCm38: Ensembl release 89: ENSMUSG00000061981 - Ensembl, maj 2017
^ "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.
^ Schroeder WT, Siciliano MJ, Stewart-Galetka SL, Duvic M (Feb 1992). "The human gene for an epidermal surface antigen (M17S1) is located at 17q11-12". Genomics. 11 (2): 481–2. doi:10.1016/0888-7543(91)90166-C. PMID 1769667.
^ ^a ^b "Entrez Gene: FLOT2 flotillin 2".
^ Hazarika P, McCarty MF, Prieto VG, George S, Babu D, Koul D, Bar-Eli M, Duvic M (Oct 2004). "Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1". Cancer Res. 64 (20): 7361–9. doi:10.1158/0008-5472.CAN-04-0823. PMID 15492257.
^ "UniProt, Q14254". Pristupljeno 21. 6. 2021.

Dopunska literatura

Schroeder WT, Stewart-Galetka S, Mandavilli S, et al. (1994). "Cloning and characterization of a novel epidermal cell surface antigen (ESA)". J. Biol. Chem. 269 (31): 19983–91. PMID 8051082.
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Suzuki Y, Yoshitomo-Nakagawa K, Maruyama K, et al. (1997). "Construction and characterization of a full length-enriched and a 5'-end-enriched cDNA library". Gene. 200 (1–2): 149–56. doi:10.1016/S0378-1119(97)00411-3. PMID 9373149.
Volonte D, Galbiati F, Li S, et al. (1999). "Flotillins/cavatellins are differentially expressed in cells and tissues and form a hetero-oligomeric complex with caveolins in vivo. Characterization and epitope-mapping of a novel flotillin-1 monoclonal antibody probe". J. Biol. Chem. 274 (18): 12702–9. doi:10.1074/jbc.274.18.12702. PMID 10212252.
Hazarika P, Dham N, Patel P, et al. (1999). "Flotillin 2 is distinct from epidermal surface antigen (ESA) and is associated with filopodia formation". J. Cell. Biochem. 75 (1): 147–59. doi:10.1002/(SICI)1097-4644(19991001)75:1<147::AID-JCB15>3.0.CO;2-D. PMID 10462713.
Salzer U, Prohaska R (2001). "Stomatin, flotillin-1, and flotillin-2 are major integral proteins of erythrocyte lipid rafts". Blood. 97 (4): 1141–3. doi:10.1182/blood.V97.4.1141. PMID 11159550.
Solomon S, Masilamani M, Rajendran L, et al. (2002). "The lipid raft microdomain-associated protein reggie-1/flotillin-2 is expressed in human B cells and localized at the plasma membrane and centrosome in PBMCs". Immunobiology. 205 (1): 108–19. doi:10.1078/0171-2985-00114. PMID 11999340.
Wistow G, Bernstein SL, Wyatt MK, et al. (2002). "Expressed sequence tag analysis of human RPE/choroid for the NEIBank Project: over 6000 non-redundant transcripts, novel genes and splice variants". Mol. Vis. 8: 205–20. PMID 12107410.
Strausberg RL, Feingold EA, Grouse LH, et al. (2003). "Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences". Proc. Natl. Acad. Sci. U.S.A. 99 (26): 16899–903. doi:10.1073/pnas.242603899. PMC 139241. PMID 12477932.
Shin BK, Wang H, Yim AM, et al. (2003). "Global profiling of the cell surface proteome of cancer cells uncovers an abundance of proteins with chaperone function". J. Biol. Chem. 278 (9): 7607–16. doi:10.1074/jbc.M210455200. PMID 12493773.
Lehner B, Semple JI, Brown SE, et al. (2004). "Analysis of a high-throughput yeast two-hybrid system and its use to predict the function of intracellular proteins encoded within the human MHC class III region". Genomics. 83 (1): 153–67. doi:10.1016/S0888-7543(03)00235-0. PMID 14667819.
Gerhard DS, Wagner L, Feingold EA, et al. (2004). "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)". Genome Res. 14 (10B): 2121–7. doi:10.1101/gr.2596504. PMC 528928. PMID 15489334.
Hazarika P, McCarty MF, Prieto VG, et al. (2004). "Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1". Cancer Res. 64 (20): 7361–9. doi:10.1158/0008-5472.CAN-04-0823. PMID 15492257.
Rual JF, Venkatesan K, Hao T, et al. (2005). "Towards a proteome-scale map of the human protein-protein interaction network". Nature. 437 (7062): 1173–8. doi:10.1038/nature04209. PMID 16189514. S2CID 4427026.
Bracho C, Dunia I, Romano M, et al. (2006). "Caveolins and flotillin-2 are present in the blood stages of Plasmodium vivax". Parasitol. Res. 99 (2): 153–9. doi:10.1007/s00436-006-0139-6. PMID 16521037. S2CID 1099026.
Doherty SD, Prieto VG, George S, et al. (2007). "High flotillin-2 expression is associated with lymph node metastasis and Breslow depth in melanoma". Melanoma Res. 16 (5): 461–3. doi:10.1097/01.cmr.0000222592.75858.20. PMID 17013097. S2CID 9758718.
Jiang M, Ding Y, Su Y, et al. (2007). "Arginase-flotillin interaction brings arginase to red blood cell membrane". FEBS Lett. 580 (28–29): 6561–4. doi:10.1016/j.febslet.2006.11.003. PMID 17113085. S2CID 20180459.
Sugawara Y, Nishii H, Takahashi T, et al. (2007). "The lipid raft proteins flotillins/reggies interact with Galphaq and are involved in Gq-mediated p38 mitogen-activated protein kinase activation through tyrosine kinase". Cell. Signal. 19 (6): 1301–8. doi:10.1016/j.cellsig.2007.01.012. PMID 17307333.
Langhorst MF, Solis GP, Hannbeck S, et al. (2007). "Linking membrane microdomains to the cytoskeleton: regulation of the lateral mobility of reggie-1/flotillin-2 by interaction with actin". FEBS Lett. 581 (24): 4697–703. doi:10.1016/j.febslet.2007.08.074. PMID 17854803. S2CID 13258838.
Rajendran L, Beckmann J, Magenau A, et al. (2009). "Flotillins are involved in the polarization of primitive and mature hematopoietic cells". PLOS ONE. 4 (12): e8290. doi:10.1371/journal.pone.0008290. PMC 2794375. PMID 20027317.
Pust S, Dyve AB, Torgersen ML, et al. (2010). "Interplay between toxin transport and flotillin localization". PLOS ONE. 5 (1): e8844. doi:10.1371/journal.pone.0008844. PMC 2809741. PMID 20107503.
Stuermer CA (2010). "The reggie/flotillin connection to growth". Trends Cell Biol. 20 (1): 6–13. doi:10.1016/j.tcb.2009.10.003. PMID 19896850.
Solis GP, Malaga-Trillo E, Plattner H, et al. (2010). "Cellular roles of the prion protein in association with reggie/flotillin microdomains" (PDF). Front. Biosci. 15: 1075–85. doi:10.2741/3662. PMID 20515742.
Ludwig A, Otto GP, Riento K, et al. (2010). "Flotillin microdomains interact with the cortical cytoskeleton to control uropod formation and neutrophil recruitment". J. Cell Biol. 191 (4): 771–84. doi:10.1083/jcb.201005140. PMC 2983060. PMID 21059848.
Cornfine S, Himmel M, Kopp P, et al. (2011). "The kinesin KIF9 and reggie/flotillin proteins regulate matrix degradation by macrophage podosomes". Mol Biol Cell. 22 (2): 202–15. doi:10.1091/mbc.E10-05-0394. PMC 3020916. PMID 21119006.
Ge L, Qi W, Wang LJ, Miao HH, et al. (2011). "Flotillins play an essential role in Niemann-Pick C1-like 1-mediated cholesterol uptake". Proc Natl Acad Sci U S A. 108 (2): 551–6. doi:10.1073/pnas.1014434108. PMC 3021008. PMID 21187433.
Song J, Chen W, Lu Z, et al. (2011). "Soluble expression, purification, and characterization of recombinant human flotillin-2 (reggie-1) in Escherichia coli". Mol Biol Rep. 38 (3): 2091–8. doi:10.1007/s11033-010-0335-4. PMID 20857209. S2CID 26736046.

[refGRCh38Ensembl-1] GRCh38: Ensembl release 89: ENSG00000132589 - Ensembl, maj 2017

[refGRCm38Ensembl-2] GRCm38: Ensembl release 89: ENSMUSG00000061981 - Ensembl, maj 2017

[3] "Human PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[4] "Mouse PubMed Reference:". National Center for Biotechnology Information, U.S. National Library of Medicine.

[pmid1769667-5] Schroeder WT, Siciliano MJ, Stewart-Galetka SL, Duvic M (Feb 1992). "The human gene for an epidermal surface antigen (M17S1) is located at 17q11-12". Genomics. 11 (2): 481–2. doi:10.1016/0888-7543(91)90166-C. PMID 1769667.

[entrez-6] "Entrez Gene: FLOT2 flotillin 2".

[pmid15492257-7] Hazarika P, McCarty MF, Prieto VG, George S, Babu D, Koul D, Bar-Eli M, Duvic M (Oct 2004). "Up-regulation of Flotillin-2 is associated with melanoma progression and modulates expression of the thrombin receptor protease activated receptor 1". Cancer Res. 64 (20): 7361–9. doi:10.1158/0008-5472.CAN-04-0823. PMID 15492257.

[UniProt-8] "UniProt, Q14254". Pristupljeno 21. 6. 2021.

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