Instructions for Use

Leica EG1150 C
Cold Plate

Leica EG1150 C V 2.6, English 07/2016

Order No.: 14 0388 80101 RevH
Always keep this manual with the instrument.
Read carefully before working with the instrument.
 NOTE

The information, numerical data, notes and val- These are determined only by the contract pro-
ue judgments contained in this manual repre- visions agreed between ourselves and our cus-
sent the current state of scientific knowledge tomers.
and state-of-the-art technology as we under- Leica reserves the right to change technical
stand it following thorough investigation in this specifications as well as manufacturing pro-
field. cesses without prior notice. Only in this way is
We are under no obligation to update the pres- it possible to continuously improve the technol-
ent manual periodically and on an ongoing ogy and manufacturing techniques used in our
basis according to the latest technical devel- products.
opments, nor to provide our customers with ad- This document is protected under copyright
ditional copies, updates etc. of this manual. laws. All copyrights to this documentation are
To the extent permitted in accordance with held by Leica Biosystems Nussloch GmbH.
the national legal system as applicable in each Any reproduction of text and illustrations (or of
individual case, we shall not be held liable for any parts thereof) by means of print, photocopy,
erroneous statements, drawings, technical il- microfiche, web cam or other methods—in-
lustrations etc. contained in this manual. In cluding any electronic systems and media—
particular, no liability whatsoever is accepted requires express prior permission in writing by
for any financial loss or consequential damage Leica Biosystems Nussloch GmbH.
caused by or related to compliance with state- For the instrument serial number and year of
ments or other information in this manual. manufacture, please refer to the nameplate at
Statements, drawings, illustrations and other in- the back of the instrument.
formation regarding the contents or technical
details of the present Instructions for Use are
not to be considered warranted characteristics © Leica Biosystems Nussloch GmbH
of our products.

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 62 24 143-0
Fax: +49 6224 143-268
Internet: http://www.LeicaBiosystems.com
Assembly contracted to Leica Microsystems Ltd. Shanghai

Leica EG1150 C 3
Table of contents

1.   Important Information................................................................................................................................. 5
1.1 Symbols in the text and their meanings................................................................................................ 5
1.2 Qualification of personnel........................................................................................................................ 6
1.3 Intended use of instrument...................................................................................................................... 6
1.4 Instrument type.......................................................................................................................................... 6
2.   Safety............................................................................................................................................................. 7
2.1 Safety notes............................................................................................................................................... 7
2.2 Warnings.................................................................................................................................................... 8
2.3 Integrated safety devices........................................................................................................................ 9
3. Instrument components and specifications.......................................................................................... 10
3.1 Overview – instrument parts................................................................................................................. 10
3.2 Technical Data......................................................................................................................................... 11
4. Instrument Setup..................................................................................................................................... 12
4.1 Location conditions................................................................................................................................. 12
4.2 Delivery range.......................................................................................................................................... 12
4.3 Unpacking and installation.................................................................................................................... 13
4.4 Power supply........................................................................................................................................... 15
5. Operation.................................................................................................................................................. 16
5.1 Switch on the instrument....................................................................................................................... 16
5.2 Replacing the secondary fuse.............................................................................................................. 16
5.3 Instrument malfunctions........................................................................................................................ 17
6. Cleaning and Maintenance................................................................................................................... 18
6.1 Cleaning the instrument......................................................................................................................... 18
6.2 Maintenance instructions...................................................................................................................... 18
7.   Warranty and Service............................................................................................................................... 19
8. Decontamination Certificate (Master)................................................................................................ 20

4 Instructions for Use V 2.6 RevH – 07/2016

 1.   Important Information

1.1 Symbols in the text and their meanings Date of manufacture

Warnings
appear in a gray box and are marked by Observe the Instructions for Use
a warning triangle .

The CE labeling shows that the prod-

Notes, i.e. important information for uct corresponds to one or more appli-
the user, appear in a gray box and are cable European directives.
marked with the symbol .

Environmental protection symbol of

(5) Numbers in parentheses refer to item the China RoHS directive. The number
(Fig. 5) numbers in illustrations or to the illus- in the symbol indicates the "Environ-
trations themselves. ment-friendly Use Period" of the prod-
uct. The symbol is used if a substance
REF Order No. restricted in China is used in excess of
the maximum permitted limit.
SN Serial number
Symbol for labeling electrical and elec-
tronic equipment in accordance with
Symbol for alternating current
Section 7 of the German Electrical and
Electronic Equipment Act (ElektroG). Ele-
The package contents are fragile and
ktroG is the law regarding the sale, return
must be handled with care.
and environmentally sound disposal of
electrical and electronic equipment.
Indicates the correct upright position of
the package. Indicates the temperature
range permitted for storing
The package must be kept in a dry en- and transporting the pack-
vironment. age.
Minimum -29 °C
It is not permitted to stack packages,
Maximum +50 °C
and no loads may be placed on top of
the package.

Manufacturer

Leica EG1150 C 5
1. Important Information

Indicates the humidity range • All laboratory personnel designated to oper-

permitted for storing and ate this instrument must read these Instruc-
transporting the package. tions for Use carefully and must be familiar
minimum 10 % r.H. with all technical features of the instrument
maximum 85 % r.H before attempting to operate it.

Tip-n-Tell indicator to moni- 1.3 Intended use of instrument

tor whether the shipment has
been transported and stored The Leica EG1150 C is a cold plate for chilling
in upright position according and blocking out histological tissue samples in
to your requirements. With paraffin blocks.
a pitch of 60° or more, the Any other use of the instrument will be consid-
blue quartz sand flows into ered as improper use!
the arrow-shaped indicator
window and sticks there per- 1.4 Instrument type
manently. Improper handling All information provided in these Instructions
of the shipment is immediately for Use applies only to the instrument type indi-
detectable and can be proven cated on the cover page.
definitively.
A nameplate with the serial number is attached
In the Shockwatch system, a to the back of the instrument.
precision glass tube shows
shocks or impacts that are
above a specified intensity
through red coloration. Ex-
ceeding a defined accelera-
tion (g value) destroys the
surface tension of the liquid
in the interior of the tube. This
causes the indicator tube to
change color.

1.2 Qualification of personnel Fig. 1

• The Leica EG1150 C may be operated by Fig. 1 is only an example. Details on

trained laboratory personnel only. your device may differ.

6 Instructions for Use V 2.6 RevH – 07/2016

 2.   Safety

The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.

2.1 Safety notes

These Instructions for Use include important in- This instrument has been built and tested in ac-
structions and information related to the oper- cordance with the safety requirements for elec-
ating safety and maintenance of the instrument. trical equipment for measurement, control, and
The Instructions for Use are an important part laboratory use.
of the product, and must be read carefully pri- To maintain this condition and ensure safe op-
or to startup and use and must always be kept eration, the user must observe all notes and
near the instrument. warnings contained in these Instructions for
Use.

These Instructions for Use must be appropriately supplemented as required by the existing reg-
ulations on accident prevention and environmental safety in the operator's country.

The protective devices located on the instrument and the accessories must not be removed or
modified. Only service personnel qualified by Leica may repair the instrument and access the
instrument's internal components.

Use only the provided power cable - this must not be replaced with a different power cable. If
the power plug does not fit in your socket, contact our service.

Residual risks
The instrument has been designed and constructed with the latest state-of-the-art technology
and according to recognized standards and regulations with regard to safety technology. Oper-
ating or handling the instrument incorrectly can place the user or other personnel at risk of in-
jury or can cause damage to the instrument or other property. The instrument may be used only
as intended and only if all of its safety features are in proper working condition. Malfunctions
that impede safety must be remedied immediately.

For current information about applicable guidelines, please refer to the CE declaration of con-
formity and on our Internet site at:
http://www.LeicaBiosystems.com

Leica EG1150 C 7
2. Safety

To prevent damage to the instrument or the specimen, only accessories authorized by Leica
may be used.

2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.

Warnings – Safety notes on the instrument itself

• The instrument may be operated by trained laboratory personnel only. It must only be oper-
ated for the purpose of its designated use and according to the instructions contained in
these Instructions for Use.
• Safety notes on the instrument itself marked with a warning triangle indicate that the cor-
rect operating instructions (as defined in these Instructions for Use) must be followed when
operating or replacing the item marked. Nonobservance can cause accidents, injuries and/
or damage to the instrument/accessories.

Safety instructions – transport, installation and operation

• After unpacking the instrument it may only be transported in an upright position.

• Before connecting the device to a power source, ensure that the voltage indicated on the
type plate matches the voltage available at the place of installation.
• The unit must be connected only with the supplied power cable and only to a grounded
power receptacle. Do not use an extension cord.
• The power socket to which the instrument is connected has to be near the instrument and
easily accessible.

8 Instructions for Use V 2.6 RevH – 07/2016

 2.   Safety

• The minimum voltage (see Technical data) must be maintained while starting the refrigera-
tion unit.
The compressor needs a start-up current of approx. 25 A. A stable power supply in accor-
dance with the instrument's specifications is essential to its proper functioning. Please en-
sure that your electrical installation fulfills these preconditions prior to installing the unit.
Nonobservance causes damage to the instrument.
• Switch off the instrument each time before servicing, repairing or cleaning, and pull out the
power plug.

2.3 Integrated safety devices

Automatic circuit breaker in the main switch
Short-circuit • An automatic circuit breaker with a lit
switch is located in the main switch. This

 circuit breaker disconnects the unit from the

power supply in the event of a short circuit.
• In this case, the main switch jumps to the
position "0" = Off.
Fig. 2

Leica EG115 0 C 9
3. Instrument components and specifications

3.1 Overview – instrument parts

• The instrument is distinguished by a simple, modular design and a
powerful refrigeration unit with precisely controlled cooling perfor-
mance.
• High temperature consistency ensures homogeneous blocking out and
prevents tearing of the paraffin blocks.
• High cooling performance ensures that the instrument's working tem-
perature is reached quickly.
• Optimized temperature distribution in the cold plate prevents dripping
condensation.
• The generously-dimensioned cooling surface has room for around 70
blocks.
• Designed for use with the Leica EG1150 H paraffin embedding station.

Refrigeration
Illuminated system
on-off switch (inside)
(automatic fuse)
Hinged hood
(optional)

Cooling surface

Adjustable feet, front

and back

Fig. 3

10 Instructions for Use V 2.6 RevH – 07/2016

 3.   Instrument Components and Specifications

3.2 Technical Data

General data
Approvals: The instrument-specific marks are located on the rear panel
of the instrument next to the nameplate.
The 120 V version has the test symbol c-CSA-us.
Voltage of power supply: Instru- Power supply Order number Coolant
The device requires a specific AC voltage, ment type voltage
see table at right.
Instrument type acc. to identification label. 230 V / 50 Hz 14 0388 38037 80 g ± 5 g
AC ±10 % 120 V / 60 Hz 14 0388 38038 80 g ± 5 g
EG1150 C
240 V / 50 Hz 14 9011 50C01 80 g ± 5 g
100 V / 50 -60 Hz 14 0388 38039 80 g ± 5 g
Power fuse: T1A L250 V
Nominal power: 230 V and 240 V instruments: 350 VA,
120 V instruments: 400 VA, 100 V instruments: 300 VA
Maximum start-up current (5 s) 25 A
Operating temperature range: +15 °C to +28 °C
Operating temperatures: -5 °C up to a room temperature of approx. +28 °C
Relative humidity: 20 to 80 % - non-condensing
Permissible temperature range -29 °C to +50 °C
during storage and transport:
Permissible humidity range 10 to 85 % - non-condensing
during storage and transport:
IEC 1010 classification: Protection class 1
Pollution degree 2
Operating elevation: up to max. 2000 m NN
IP protection class (IEC 60529): IP20
Refrigeration unit
Refrigeration capacity*: 110 W
Safety factor: 3
Refrigerant: R 134a
Compressor oil: 180 cm3 Emkarate RL 15 s, ICI
*according to CECOMAF, fluid temperature: 55 °C, evaporation temperature: -25 °C

Dimensions and weights

Width: 360 mm
Depth: 650 mm
Height: 360 mm
Weight: 23 kg
Leica EG115 0 C 11
4. Instrument Setup

4.1 Location conditions

• Stable, vibration-free laboratory table with horizontal, flat table top, as
far as possible vibration-free ground.
• No direct sunlight or strong temperature fluctuations. Room tempera-
ture consistently between +15 °C and +28 °C.
At a room temperature of > +28 °C, the working temperature of the
cold plate of -5 °C may not be reached at all points.

• Relative air humidity maximum 80 %, non-condensing.

• The instrument should be set up in such a way that the air circulation
is not impaired.

To ensure proper function, there must be gap of at least 15 cm be-

hind the instrument. Failure to observe this distance may result in
serious damage to the refrigeration unit of the device. The instru-
ment should not be operated in hazardous locations.

4.2 Delivery range

The basic equipment for the Leica EG1150 C cold plate contains the fol-
lowing components:
Order No.
• 1 basic unit Leica EG1150 C
only for Australia:
• 1 series transformer, 230/240 V 14 0469 31998
• 1 replacement fuse T 1.0 A, 6.3 x 32 mm 14 6000 01483
• 1 Instructions for Use German/English
(including CD and ordering information) 14 0388 80001
• Optional: Unit cover (acrylic)
The country specific power cord needs to be ordered separately.
Please find a list of all power cords available for your device on our
website www.LeicaBiosystems.com within the product section.
Please compare the delivered components against the packing list
and your order.
Should there be any discrepancy, please contact the Leica distribu-
tor handling your order.

12 Instructions for Use V 2.6 RevH – 07/2016

 4.   Instrument Setup

4.3 Unpacking and installation

The unpacking instructions are located on the outside of the transport box in which the
­instrument is delivered shows the assembly of the original packaging.
The numbers indicate the sequence of disassembling and reassembling.

When the instrument is delivered, check the tilt indicators on

the packaging.
If the arrowhead is blue, the shipment was transported laying
flat, was tilted at too great an angle or fell over during transport.
Note this on the shipping documents and check the shipment
for possible damage.

These unpacking instructions

1 2 only apply if the box is placed
with the symbols facing
­upwards for unpacking.

1. Remove and lift off 8x screws (1) for the

lid (2).

The Leica EG1150 C always has

to be transported upright and ho-
rizontally. It must not be inverted
under any circumstances, even
for short periods, or stored on
one of its sides.
It is mandatory to observe a wai-
3 ting time of 4 hours between the
Fig. 4

last transport and the first time

6 the instrument is switched on.
The compressor oil moved du-
ring transport must be able to
5
flow completely back into its ori-
4 ginal position.
6 3 2. Remove upper pad (3).
3. Remove the accessories (4).

Leica EG115 0 C 13
4. Instrument Setup

4. Lift the EG1150 C instrument (5) from the

bottom pad (6). Also grab the instrument
with one hand down and at the front (7)
and with the other hand up and at the
back (8) and lift it straight from the trans-
port crate.
7 8
The packaging must be retained
for the duration of the warranty
period.
Fig. 5 To return the instrument, follow
the instructions above in reverse
order.

14 Instructions for Use V 2.6 RevH – 07/2016

 4.   Instrument Setup

4.4 Power supply

The Leica EG1150 C refrigeration unit requires a specific voltage and fre-
quency (refer to the "Technical Data" chapter), and is therefore always
delivered with a power cable that fits the instrument.
Observe these instructions to prevent damage to the instrument:

Before connecting the instrument to the power supply, it is manda-

tory to check whether the voltage specified on the identification la-
bel (rear side) matches the actual voltage values at the installation
location.
If this is not the case, the connection must not be made!
The unit must be connected only with the supplied power cable and
only to a grounded power receptacle.
Do not use an extension cord!

• Connect the power cord plug (7) to the connecting port (6).
• Plug the power cord into the wall outlet.

Fig. 6

Leica EG115 0 C 15
5. Operation

5.1 Switch on the instrument

After installation as described in Chapter 4, the Leica EG1150 C is ready for
operation. Switch on the device with the On-Off switch at the front left of
the instrument ("I" = ON). A lamp lights up in the switch to indicate that the
unit is operational and the refrigeration unit will start working.
Depending on the room temperature, the working temperature of the cool-
ing surface (-5 °C) will be reached in around 25 minutes (see also Ch. 4.1).
The cooling surface may not be loaded with molds until the cooling
time has elapsed. Otherwise, the working temperature of -5 °C may
not be reached.

5.2 Replacing the secondary fuse A miniature fuse to protect the electronic com-
ponents is located on the rear of the instrument.

Before replacing the fuse, always

switch the instrument off and pull the
power plug from the wall socket.
Only miniature fuses of the type speci-
fied in the chapter "Technical data"
may be used.

To replace the fuse, please proceed as follows:

• Using a screwdriver, lightly press the fuse
holder (9) and turn counterclockwise about
1
/4 turn.
9 • Remove the fuse holder and take out the
10
fuse (10).
• Replace it with a new fuse of the same type.
• Replace the fuse holder (9).
• Using a screwdriver, gently press the fuse
holder and turn it clockwise about 1/4 turn.
• Reconnect the instrument to an AC power
9 outlet and switch it on.

Fig. 7

16 Instructions for Use V 2.6 RevH – 07/2016

 5.   Operation

5.3 Instrument malfunctions

If you cannot solve your problem using the help in the following table, please contact your Lei-
ca customer service organization or the Leica dealer from whom you purchased the instrument.

Malfunction Possible causes Corrective action

Instrument doesn't work. Power switch not turned on. Check instrument is connected,
or then turn main power switch
The cut-out function of the on.
power switch activated. Only for Great Britain:
Check the fuse in the power
plug.
Cooling unsatisfactory, Room temperature too high or Check the cooling again at a
formation of condensation. exposure to direct sunlight. lower room temperature.
Inadequate air supply to venti- Check distance of instrument to
lation unit. wall; it must be at least 15 cm.
Refrigeration system leak. The instrument must be ser-
viced or repaired by a service
technician.
Please contact customer
service.
Compressor switches off after AC power does not match Check the voltage of power
a brief period of operation specifications or supply.
and switches back on shortly Compressor defective.
thereafter.
Compressor does not start. Compressor defective. The instrument must be ser-
Compressor switches off after Compressor defective. viced or repaired by a service
a short period of operation. technician.
Please contact customer
service.

Leica EG115 0 C 17
6. Cleaning and Maintenance

6.1 Cleaning the instrument

Switch off the instrument each time before cleaning and pull out the power plug.
While handling cleaning materials, observe the safety regulations of the manufacturer and the
lab regulations valid in the country of use.
During cleaning, do not allow any liquid to penetrate inside the instrument!
To prevent scratching the surface of the instrument, do not use metallic tools with sharp edges
under any circumstances.
Work surface
• All common laboratory cleaning products suitable for the removal of paraffin (e.g. Para-
guard or xylene substitutes) can be used to clean the work area.

Instrument and outer surfaces

• If necessary, clean the painted exterior surfaces with a mild household cleaner or
soapy water and wipe with a damp cloth.
• Avoid prolonged contact of organic solvents on the surface of the instrument. Xylene or
acetone will damage the finished surfaces!

6.2 Maintenance instructions

Only Leica service technicians are authorized to open the instrument for maintenance and re-
pair work.

Please observe the following points to ensure the instrument's reliable function over ex-
tended periods:
• Clean the instrument with care daily.
• Regularly remove dust from the ventilation slots on the back of the instrument with a
brush or vacuum cleaner.
• Have the instrument inspected at least once a year by an authorized Leica customer
service technician.
• Enter into a service contract at the end of the warranty period. For more information,
contact the relevant Leica customer service organization.

18 Instructions for Use V 2.6 RevH – 07/2016

 7.   Warranty and Service

Warranty
Leica Biosystems Nussloch GmbH guarantees that the contractual prod-
uct delivered has been subjected to a comprehensive quality control
procedure based on the Leica in-house testing standards, and that the
product is faultless and complies with all technical specifications and/or
characteristics warranted.
The scope of the warranty is based on the content of the concluded
agreement. The warranty terms of your Leica sales organization or the or-
ganization from which you have purchased the contractual product shall
apply exclusively.

Service information
If you are in need of technical customer support or spare parts, please
contact your Leica representative or the Leica dealer where you pur-
chased the instrument.
Please provide the following information:
• Model name and serial number of the instrument.
• Location of the instrument and name of the person to contact.
• Reason for the service call.
• Date of delivery.

Decommissioning and disposal

The instrument or parts of the instrument must be disposed of in compli-
ance with the local laws.

Leica EG115 0 C 19
Decontamination
8.Decontamination CertificateCertificate
(Master)
Dear Customer,
Any product that is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. Since it is not possible to
decontaminate for prion diseases, such as CJD, BSE, CWD etc., equipment exposed to specimens containing prion diseases cannot be returned to Leica Biosystems
for repair. On-site repair of prion contaminated equipment will only be conducted after the Field Service Engineer has been educated in the risks, instructed in the
policies and procedures of the institution, and provided with personal protective equipment. Please fill out this confirmation carefully and enclose a copy with the
instrument. Attach the confirmation to the outside of the flight case or hand it directly to the service technician.
Packages will not be opened, nor servicing commenced until the Company or service engineer have received a satisfactory certificate. Should returned
goods be considered a hazard by the Company, they will be returned immediately to the customer at his/her expense. Note: Microtome knives must be in boxes.
Mandatory information: Fields marked with * are mandatory. Depending on whether the instrument is contaminated, please also complete either section A
or section B.
Nameplate information Model (see nameplate)* SN (see nameplate)*

REF (see nameplate)*

Tick Box A if applicable. Otherwise please complete all parts of B, providing further
information as requested or appropriate.

A
Yes
This equipment has not been in contact with unfixed biological samples.

1 This equipment has been exposed internally or externally to hazardous materials as indicated
B Yes No
below:

Blood, body fluids, pathological samples

Please provide further detail here:

Other biohazards
Chemicals/substances hazardous to health
Other hazards
Radioactivity

Yes No 2 This equipment has been cleaned and decontaminated:

Please provide further detail here:
If yes, give details of the method:

If no**, please indicate why not:

** Such equipment must not be returned without the written agreement of Leica Biosystems.
Yes No
3 The equipment has been prepared to ensure safe handling/transportation.
Whenever possible, please use the original transportation case/box.
Important - to avoid refusal of shipment:
Place one copy in the unit prior to packaging, or hand it over to the service engineer. Customer assumes all responsibility for the
immediate return shipment of articles sent to Leica without proper decontamination documentation.
If you have any further questions, please call your local Leica organization.
Leica Internal Use: If applicable, note corresponding Job and RAN-/RGA-Number:
Job Sheet No.: _______________ BU Return Authorization Number: ______________ SU Return Goods Authorization: ______________
Signature/Date* Institute*
.5 - 01/2013, Order-No. 14 0700 83101

Department*
Name*
20 Instructions for Use V 2.6 RevH – 07/2016
Address*
Position*
 ** Such equipment must not be returned without the written agreement of Leica Biosystems.
8.   Decontamination Certificate (Master)
Yes No
3 The equipment has been prepared to ensure safe handling/transportation.
Whenever possible, please use the original transportation case/box.
Important - to avoid refusal of shipment:
Place one copy in the unit prior to packaging, or hand it over to the service engineer. Customer assumes all responsibility for the
immediate return shipment of articles sent to Leica without proper decontamination documentation.
If you have any further questions, please call your local Leica organization.
Leica Internal Use: If applicable, note corresponding Job and RAN-/RGA-Number:
Job Sheet No.: _______________ BU Return Authorization Number: ______________ SU Return Goods Authorization: ______________
Signature/Date* Institute*

Version 3.5 - 01/2013, Order-No. 14 0700 83101

Department*
Name*

Address*
Position*

eMail Phone* Fax

Leica Biosystems Nussloch GmbH Phone: ++49 (0) 6224 143 0

Heidelberger Str. 17-19 Fax: ++49 (0) 6224 143 268
69226 Nussloch, Germany www.LeicaBiosystems.com

Leica EG115 0 C 21
Notes

22 Instructions for Use V 2.6 RevH – 07/2016

www.LeicaBiosystems.com

Leica Biosystems Nussloch GmbH

Heidelberger Str. 17 - 19
D-69226 Nussloch
Germany
Phone: +49 6224 143-0
Fax: +49 6224 143-268
Internet: http://www.LeicaBiosystems.com