Tpc. Ao
Tpc. Ao
Tpc. Ao
1
Himachal Institute of Pharmacy, Paonta Sahib District Sirmour.
2
Manav Bharti University, Solan, Himachal Pradesh.
3,4
SBSPGI, Balawala Dehradun.
KEYWORDS: Roylea elegans, Total phenolic content, total flavonoid content, antioxidant
activity.
INTRODUCTION
In nature plants gives more products which are useful for human beings. These natural
products are used for several diseases and also used as antioxidant. Plants give secondary
metabolites which are used for several diseases. These secondary metabolites are alkaloids,
glycosides, terpenoids, flavonoids, phenolic compound and tannins. These secondary
metabolites are produced from various parts of plants like stem, leaves, flowers, fruits and
roots. These secondary metabolites are biologically active and show better activity. Flavonoid
and phenolic content shows better antioxidant activity.
Roylea elegans a plant belonging to family lamiaceae shows better antioxidant activity due to
presence of good flavonoid content. Roylea elegans is a shrub of monotypic genus. The
family lamiaceae contain 22 species. Plant Roylea elegans belonging to family lamiaceae.
Leaves part of this plant (decoction) is traditionally used as a bitter tonic and also as a
febrifuge. It is also used as a tonic in contusions. Leaves are used in skin disease and fever.
The leaves of Roylea elegans contains various phytoconstituents like betulin, beta-sitosterol,
beta-amyrin, glucose, sti gmasterol, cetyl alcohol, and palmitic, stearic, oleic, gallic, oxalic,
fructose, arabinose and tartaric acids.
The preliminary phytochemical screening of the aerial parts extract mainly done for the
evaluation of the various phytoconstituents such as steroids, tannin, Alkaloids, Flavonoids
and glycosides were present in the aerial parts of different extracts of R. elegans.
Total phenolic content was calculated with the help of standard curve equation and the
formula was below
Total flavonoid content was calculated with standard curve equation and the formula
Antioxidant activity
DPPH
DPPH will be assayed as described by Elizabeth and Rao. The reaction mixture contained 1.0
ml of 0.3mM DPPH in 50 ml of methanol will be added with concentrations ranging from 20-
100 μg/ml .The mixture of DPPH in methanol used as positive control and methanol alone
served as blank. When DPPH reacts with antioxidants in the sample, it was reduced and the
colour changed from deep violet to light yellow and measured at 517 nm. Quercetin will be
used as a reference standard. Scavenging activity (%) = [A517 (control) – A517 (sample)/
A517 (control)] X 100.[4]
1. 0.1 0.292
2. 0.2 0.329
3. 0.4 0.402
4. 0.8 0.614
5. 1.6 0.869
% Inhibition
S. Concentration
Petroleum Ethyl
no. (µg/ml) Rutin Chloroform Methanol Water
ether acetate
1. 40 28.73 0.74 2.98 1.49 12.31 0.37
2. 60 49.25 6.34 4.85 10.44 19.02 3.35
3. 80 55.59 11.56 7.46 17.16 21.64 20.89
4. 100 63.43 15.67 10.82 29.10 24.25 29.85
CONCLUSION
In our present study total phenolic content of in different extracts were showing that water
extract has high phenolic content and total flavonoid content of different extract showing
ethyl acetate has high. Total antioxidant activity of different e extracts was showing that ethyl
acetate has high antioxidant power due to high flavonoid content.
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