UVD 170U and UVD 340U UV/VIS Detectors: Operating Instructions

UVD 170U and UVD 340U
UV/VIS Detectors
Operating Instructions
Revision: 1.0-a
Date: July 2003
© 2003 Dionex
Doc.: UVD170U340U_OI_E_V101a.doc
UVD170U/340U Operating Instructions
Declaration of Conformity
Product: UV/VIS Detector
Type: UVD 170U/340U
Dionex GmbH herewith declares conformity of the above products with the respective
requirements of the following regulations:
• Low-Voltage Equipment Directive 73/23/EEC

changed by 93/68/EEC
• EMC Directive 89/336/EEC
changed by 91/263/EEC; 92/31/EEC; 93/68/EEC
The electrical safety of the products was evaluated based on the following standard:
• EN 61010-1:1993
Safety requirements for electrical equipment for measurement, control, and
laboratory use
Part 1: General Requirements
The Electromagnetic Compatibility (EMC) of the products was evaluated based on the
following standards:
• EN 50081-1:1992:
Electromagnetic Compatibility (EMC) - Generic emissions standard
Part 1: Residential, commercial, and light industry
• EN 50082-1:1992:
Electromagnetic Compatibility (EMC) - Generic immunity standard
Part 1: Residential, commercial, and light industry
• EN 61000-3-2:1998
Electromagnetic Compatibility (EMC)
Part 3 / Section 2: Limits for harmonic current emissions
This declaration is issued for the manufacturer

Dionex GmbH
Dornierstrasse 4
D-82110 Germering
by the President, Dr. Peter Jochum.

September 27, 2001
Contents
1 Introduction ....................................................................................................................... 1
1.1 How to Use This Manual .............................................................................................. 1
1.2 Unpacking ..................................................................................................................... 2
1.3 Warranty ....................................................................................................................... 2
1.4 Intended Use ................................................................................................................. 3
1.5 Federal Communications Commission (FCC) Note ..................................................... 3
2 Overview............................................................................................................................. 5
2.1 Principle of Operation................................................................................................... 5
2.2 Flow Cells ..................................................................................................................... 6
3 Installation.......................................................................................................................... 7
3.1 Positioning and Environment........................................................................................ 7
3.2 Electrical Connection.................................................................................................... 8
3.2.1 Setting the Voltage Selector................................................................................... 8
3.2.2 Replacing the Fuses.............................................................................................. 10
3.3 Rear Panel Connections .............................................................................................. 11
3.4 Fluid Connections ....................................................................................................... 13
4 Operation.......................................................................................................................... 15
4.1 Power-Up .................................................................................................................... 15
4.2 Front Panel Status LED .............................................................................................. 15
4.3 Operation after Power Failure..................................................................................... 15
5 Automated Control by Chromeleon .............................................................................. 17

5.1 General........................................................................................................................ 17
5.1.1 USB Installation ................................................................................................... 17
5.1.2 LAN Installation................................................................................................... 18
5.1.2.1 Assigning IP Properties to the Detector (CmIPUtil) ..................................... 24
5.1.2.2 Recommendations for Network Operation.................................................... 26
5.2 Installing the Detector in Chromeleon........................................................................ 27
5.3 Operation with Chromeleon........................................................................................ 31
5.4 Wavelength Calibration under Chromeleon ............................................................... 33
5.5 System Wellness and Reliability ................................................................................ 33
6 Troubleshooting............................................................................................................... 35
6.1 The Most Frequently Problems................................................................................... 35
6.2 The Most Frequent Error Messages............................................................................ 37
7 Maintenance ..................................................................................................................... 39
7.1 General Information.................................................................................................... 39
7.2 Deuterium Lamp ......................................................................................................... 41
7.2.1 Removing the Lamp ............................................................................................. 41
7.2.2 Installing the Lamp............................................................................................... 41
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7.3 Flow Cell..................................................................................................................... 42

7.3.1 Removing the Flow Cell ...................................................................................... 43
7.3.2 Installing the Flow Cell ........................................................................................ 43
7.3.3 Cleaning the Flow Cell......................................................................................... 43
8 Upgrade Options.............................................................................................................. 45
9 Technical Specifications .................................................................................................. 47
10 Accessories and Spare Parts........................................................................................ 49

10.1 Standard Accessories (included in the shipment).................................................... 49
10.2 Optional Accessories and Spare Parts ..................................................................... 50
11 Index .............................................................................................................................. 53
ii
1 Introduction
1.1 How to Use This Manual
The layout of this manual is designed to provide quick reference to the sections of interest to
the user. However, in order to obtain full understanding of the detectors, we recommend that
you review the manual thoroughly before beginning operation. All descriptions in the manual
apply to both the UVD 170U and UVD 340U detector. Therefore, the term "the detector" is
used. If some detail applies to only one of the detectors, the detector is identified by name.
At various points throughout the manual, messages of particular importance are indicated by the
following symbols:
Please note: Indicates general information to optimize the performance of the

instrument.
Important: Indicates that failure to take note of the accompanying information may
result in damage to the instrument.
Warning: Indicates that failure to take note of the accompanying information may
result in personal injury.
This manual is provided "as is". Every effort has been made to supply complete and accurate
information and all technical specifications have been developed with the utmost care. However,
Dionex assumes no responsibility and cannot be held liable for any errors, omissions, damage, or
loss that might result from any use of this manual or the information contained therein. We
appreciate your help in eliminating any errors that may appear in this document.
The information contained in this document is subject to change without notice.
CHROMELEON® and Summit® HPLC are registered trademarks of Dionex Corp. All other
trade or company names mentioned are subject to the copyright and the property and trademark
rights of the respective companies.
All rights reserved, including those for photomechanical reproduction and storage on electronic
media. No part of this publication may be copied or distributed, transmitted, transcribed, stored
in a retrieval system, or transmitted into any human or computer language, in any for or by any
means, electronic, mechanical, magnetic, manual, or otherwise, or disclosed to third parties
without the express written consent of Dionex Corp.
1
1.2 Unpacking
All electrical and mechanical components of the detector are carefully tested before the
instrument is shipped. After unpacking, please check the detector for any signs of mechanical
damage, which might have occurred during shipment.
Please note: Immediately report any shipping damage to both the incoming carrier and
Dionex. The shipping insurance will compensate for the damage only if
reported immediately.
Please note: Keep the original packing material, as it is the optimum packaging for
shipping the unit (e.g., for repair). Shipping the unit in any other packaging
automatically voids the product warranty.
To unpack the detector, proceed as follows:
• Place the shipping container on the floor and remove the accessories pack.
• Grasp the detector by the sides. Slowly and carefully, pull it out of the shipping container and
place it on a stable surface.
• Remove the foam inserts, and then remove the polythene packaging.
• Check off the contents of the accessory pack against the list in the Standard Accessories
section, page 49.
1.3 Warranty
The standard warranty coverage for the unit is in accordance with the terms of sale. Warranty
is given for one year, starting from the invoice date, and covers materials and labor, ex-works.
Please note that worn-out parts are not covered by the warranty.
The warranty period for the deuterium lamp is six months, starting from the delivery date (see
label at lamp cable). Removing the label automatically invalidates the warranty. The warranty
period for lamps with timer (without the above label) is 1000 operating hours.
The warranty coverage does not include the flow cell.
The warranty coverage shall become invalid in any case identified as resulting from
inappropriate use, service, or the implementation of non-specified spare parts. Similarly, the
warranty coverage shall be invalidated in the event of inappropriate shipment, packaging, or
failure to remove aggressive or damaging solvent residues.
2
1.4 Intended Use

The detector is a highly sensitive UV-VIS diode array detector designed to perform HPLC
analyses with the Chromeleon Chromatography Management System. The UVD 340U
detector allows the recording of three-dimensional data, in addition to the UV-VIS channels
available on the UVD 170U detector.
Please note that the unit may be operated only using the accessories originally supplied with the
detector (→ page 49) and within its technical specifications (→ page 47).
If there is a question regarding appropriate usage, contact Dionex before proceeding.
Dionex shall not be liable for any damage, material or otherwise, which is caused by
inappropriate or improper use of the device.
1.5 Federal Communications Commission (FCC) Note

This equipment has been tested and found to comply with the limits for a Class A digital
device, pursuant to part 15 of the U.S. FCC Rules. These limits are designed to provide
reasonable protection against harmful interference when the equipment is operated in a
commercial environment. This equipment generates, uses, and can radiate radio frequency
energy and, if not installed and used in accordance with the instruction manual, may cause
harmful interference to radio communications. Operation of this equipment in a residential
area is likely to cause harmful interference, in which case the user will be required to correct
the interference at his expense.
3
4
2 Overview
2.1 Principle of Operation
The UVD170U and 340U detectors feature:
• Highest sensitivity
• Powerful optics
• Extremely low-noise electronics
• Simultaneous detection at four wavelengths
• Recording of three-dimensional data
Please note: Recording of three-dimensional data is only possible with the

UVD 340U detector and the corresponding Chromeleon PDA
license.
• Maximum analysis efficiency, due to comprehensive data analysis capabilities in

Chromeleon.
Fig. 1: Principle of operation
5
2.2 Flow Cells
Important: The chemical resistance of the flow cell depends on the solvents used for
the analysis. This applies particularly to solvents that can form active
radicals or peroxides.
Please note: All UVD 170/340-U detectors are shipped with a standard analytical flow
cell (part no. 5065.1810).
The standard analytical flow cell (→ Fig. 21, page 42) is the choice for applications using 3
and 4 mm I.D. columns. The cell consists of a PEEK cell body and a heat exchanger equipped
with a stainless steel capillary with an inner diameter of 0.25mm I.D. The inlet capillary is
factory-fitted with a single-part, hand-tight fitting. The flow outlet capillary is made of PEEK
and has an inner diameter of 0.50 mm. The cell volume is 10 µL and the cell path length is
9 mm. The maximum pressure limit of the standard analytical flow cell is 10 MPa (1450 psi).
For other fields of application, the following flow cells are available as an option:
• Biocompatible flow cell (Part no. 5065.1820)
The biocompatible flow cell is recommended for applications using stainless steel
incompatible eluents or analytes. It has almost the same design as the standard analytical flow
cell. However, the stainless steel capillary is substituted by a PEEK capillary with an inner
diameter of 0.25 mm. The cell volume is 10 µL and the cell path length is 9 mm. The
maximum pressure limit for the biocompatible flow cell is 10 MPa (1450 psi).
• Semi-Preparative flow cell (Part no. 5065.1800P)
This flow cell is recommended for semi-preparative applications using columns with an inner
diameter, for example, of 25 mm. Often, highly concentrated samples and/or large injection
volumes are used, resulting in high signal levels with standard cell path length, e.g., 9 mm.
The path length of the semi-preparative flow cell is 2 mm. It has almost the same design as
the standard analytical flow cell. However, the cell body is made of PCTFE. The inlet and
outlet capillaries, with an inner diameter of 0.7 mm, are made of stainless steel. The cell
volume is 6 µL and the maximum pressure limit for this flow cell is 10 MPa (1450 psi).
For applications with 2 mm I.D. columns, we recommend using the micro flow cell:
• Micro flow cell (Part no. 5065.1810M)
The micro flow cell has almost the same design as the standard analytical flow cell. The cell
volume is 2.5 µL and the cell path length is 9 mm. The maximum pressure limit for the micro
flow cell is 10 MPa (1450 psi).
For applications with 1 mm I.D. columns, we recommend using the fused silica nano flow cell
or the micro flow cell:
• Fused silica nano flow cell (Part no. 5065.1810N)
Use the fused silica flow cell for applications for which peak separation and thus, the peak
width is critical. The cell volume is 395 nL and the path length is 8 mm. The maximum
pressure limit for the fused silica flow cell is 40 MPa (5800 psi).
Please keep in mind that fused silica might be incompatible with special eluents, e.g.,
pH ≥ 12. In these cases, use the micro flow cell (see above).
For information about how to clean the flow cells, refer to section 7.3.3 (→ page 43).
6
3 Installation
3.1 Positioning and Environment
Bring the detector to a moderate temperature for four hours to allow any condensation that
might have occurred during shipping to evaporate. Do not connect the instrument to the mains
yet. After four hours, check the instrument; if condensation is still there, allow the instrument
to continue to warm up (without connecting it to the mains) until the condensation is
completely gone.
Place the instrument on a firm and level surface that is free of vibration. Avoid large
temperature variations in order to minimize the baseline drift. Avoid draughts and direct
sunlight. Allow sufficient clearance behind the detector for power connections and
ventilation. Capillary connections between the column and the flow cell should be as short as
possible, to avoid peak broadening effects due to excessive dead volume.
Solvent Rack
P680
UVD 170U/340U
ASI-100
TCC-100
Fig. 2: Summit HPLC system with detector
Important: Do not place any objects or stack additional instruments on top of the
detector. The ventilation slits on top of the unit must remain unobstructed
at all times.
7
3.2 Electrical Connection
3.2.1 Setting the Voltage Selector
Dionex instruments are factory-set for operation at the local voltage requirements of the
destination country.
Fig. 3: Factory-set voltage selection, here: 230V
If the setting is not appropriate for your local requirements, reset the voltage at the selector on
the instrument's rear panel (next to the main supply connector) as explained below:
Warning: Before resetting the voltage, turn off the detector. Be sure to disconnect the
power cord from its source.
• Remove the fuse cartridge using a small screwdriver (→ Fig. 4).
Fig. 4: Fuse cartridge
• Use small pliers or tweezers to pull the small voltage selector board out of the power socket
housing (→ Fig. 5).
Fig. 5: Voltage selector board
• Place the board (with the writing face up) on a firm surface.
• Turn the board so that you see the correct voltage selection, as shown in → Fig. 6.
• Without turning the board, adjust the plastic clip as required for the mains voltage (→ Fig. 6).
Make sure that the clip locks into position.
8
a) b)
Fig. 6: Voltage selector board settings
a) for 230 V and b) for 115V
Please note: Set the voltage selector as follows:

For a voltage of 90V-130V: Set the selector to 100 or 120
For a voltage of 200V-240V: Set the selector to 230 or 240.
• Reinstall the voltage selector board in the power socket housing.

• If necessary, install new fuses of the correct rating (→ page 10).
• Reinstall the fuse cartridge and check that the correct voltage is set.
Important: During initial installation of the detector, check the correct power supply,
the grounding, and the fuses.
Please note: For minimum interference effects, all components of the analytical system
should be connected to the same mains output (same phase).
9
3.2.2 Replacing the Fuses
To replace the fuses, follow the steps below:
Warning: Before replacing the fuses, turn off the detector. Be sure to disconnect the
power cord from its source.
• Remove the fuse cartridge, using a small screwdriver (→ Fig. 4).

• Replace the fuses with fuses of the appropriate rating.
Fig. 7: Fuses
Important: Use only the fuses indicated below or those listed in the Optional
Accessories and Spare Parts list (→ page 50).
Voltage Description Part-Nr.

230V* Fuse 1A, slow, 5x20mm, 250V Included in "UVD Fuses Kit", part no. 6065.9002
115V** Fuse, 2A, slow, 5x20mm, 250V
* The voltage selector is set to 230V or 240V.
** The voltage selector is set to 100V or 120V.
• Reinstall the fuse cartridge.

• Reconnect the power cord to its source and turn on the detector.
10
3.3 Rear Panel Connections
The Chromeleon Chromatography Management

System can use either a LAN or USB connection to
control the detector.
Data transfer is digitally via the respective cables

(USB cable, part no. 8911.0002; LAN cable, part
no. 8906.2038) that are connected to the instrument's
rear panel.
Important: To ensure trouble-free operation,

only use the original connection
cables (see above) and USB
extension cables (part no.
8911.0004) that are available from
Dionex.
For more information about the single ports, see below.

(2) (1)
Fig. 8: Rear panel view
USB
The detector is connected to the server PC via the USB port (1), i.e., either directly to the USB
port of the PC, or via another instrument of the Summit HPLC product line that is already
connected to the server PC, or via a USB hub.
The other three USB ports (2) allow the detector to be connected to either one instrument of the
Summit HPLC line or one USB hub each. Additional Summit HPLC instruments can then be
connected to the hub.
Important: We recommend using these USB ports (2) for connecting Dionex
instruments only. Dionex cannot guarantee correct functioning if
instruments of other manufacturers are connected.
The two digital inputs can be used to inform Chromeleon about external events. The relay
outputs allow potential-free switching of external signals.
11
LAN
The LAN port allows connection of the detector to an instrument LAN.
Digital I/O
The two 6-pin ports (Mini-DIN) on the detector rear panel can be used under Chromeleon, as
necessary, for communication with external instruments, e.g., to synchronize the Inject Signal of
an external autosampler or injection valve with the start of the analysis start by the data system.
Please note: If the detector is connected to Chromeleon via LAN, it is not advisable to
use the digital inputs for remote inject synchronization. This is because the
timing of LAN communication varies, depending on the current LAN
traffic.
Fig. 9:Digital I/O port (View from the rear)
Range
Digital Input ↔ Digital GND 0 or +5V
Digital Out (GND) ↔ Digital Output Switching voltage: 100V; switching current: 0.5A
Limiting value of mean on-state current: 1.0A
Switching capacity: 10W/10VA
Volume resistance: max. 0.15 Ohm
A 6-pin signal cable (Mini-DIN) (part no. 8911.0001) is available for connection. The Analog
High, Analog Low, and Shield pins on the connection cable are not supported.
12
3.4 Fluid Connections

The capillary connections of the detectors are located on the left side of the detector
(→ Fig. 20). The input capillary is black. Connect the capillary to the column output.
Important: The maximum pressure stability of the flow cell depends on the model.
The maximum pressure limit for the standard flow cell is 10 MPa
(1450 psi). For information about the pressure stability of the other flow
cell that are provided for the UVD-U detectors, refer to Technical
Specifications (→ page 47).
Important: The flow cell is primed with 2-propanol.
Please note: Make sure that capillary connections between the column and the flow cell
are as short as possible. This will avoid peak broadening effects due to
excessive dead volume.
13
14
4 Operation
The detector is intended for operation with the Chromeleon Chromatography Management
System. Stand-alone operation is not possible. Data transfer is performed digitally, via USB
or LAN (→ Rear Panel Connections, page 11 and USB Installation, page 17 and/or LAN
Installation, page 18).
4.1 Power-Up
The detector is turned on via the power switch on the rear panel of the instrument.
Please note: The deuterium lamp does not switch on automatically when the power to
the detector is turned on. Lamp on/off control is via Chromeleon. For
optimum results, turn on the lamp at least 30 minutes before beginning an
analysis.
Please note: After selecting the Lamp off command, wait at least 5 minutes before
turning on the deuterium lamp again. Otherwise, the lamp becomes too hot
and may be damaged (message "Lamp too hot...").
4.2 Front Panel Status LED

The two-colored front panel LED indicates the status of the detector when the power is turned
on:
LED Status
A red light is flashing. The detector is not yet connected to
Chromeleon.
A red light is burning. The detector is connected to Chromeleon
(firmware started).
A green light is flashing. Deuterium lamp is preheating.
A green light is burning. Deuterium lamp is on.
4.3 Operation after Power Failure

If the HPLC system is operated under Chromeleon, the system can be programmed via the
corresponding program file (PGM File) in such a way that operation is started again as desired
after a power failure. For more information, see the Chromeleon online Help and the
Chromeleon manual.
15
16
5 Automated Control by Chromeleon

5.1 General
If desired, Chromeleon, the Dionex Chromatography Management System, can control the
detector. The detector must be connected to the Chromeleon PC or an Ethernet LAN via the
USB or LAN port on the instrument's rear panel.
To control the detector by Chromeleon, one of the following Chromeleon licenses is required:
• For Chromeleon ≤ 6.40:

Basic license: to record the pure signal without spectra
or
Basic license + Device Control license: to execute time-dependent commands. This license
authorizes control of an entire chromatography
system.
or
Basic license + Device Control license + PDA license: to record a three-dimensional data
field (spectra acquisition).
This license is available only for the UVD 340U.
• For Chromeleon ≥ 6.50:
Timebase Class 1 or
Timebase Class 1 + PDA license: to record a three-dimensional data field (spectra
acquisition).
This license is available only for the UVD 340U.
For questions regarding the individual options, please contact your Dionex sales
representative.
We generally recommend connecting the detector to the Chromeleon server PC via USB
(Universal Serial Bus). Almost all PCs provide at least one USB port. However, if this is not
possible, use a network connection (LAN) for communication between the detector and the
Chromeleon server. For example, Windows NT 4.0 does not support USB. If you are using a
LAN connection, please observe the recommendations for network operation (→ page 26).
For information about how to install the detector via USB and LAN, refer to USB Installation
(see below) and LAN Installation (→ page 18).
5.1.1 USB Installation
Please note: Install the Chromeleon software (→ page 27) before connecting the
detector to the USB port of the Chromeleon server PC.
Connect the detector to the mains using the power cord shipped with the instrument. Please
observe the information about the facility requirements and the electrical connection (→ page 8).
Use the USB cable from the standard accessories kit (page 49) to connect the detector to the
USB port on the Chromeleon server PC.
17
Please note: The USB connection to the PC or the USB hub must not exceed 5m.
Longer connections are possible via a special USB extension cable
(Dionex part no. 8911.0004). Up to five extension cables may be
connected in series. For system reasons, the overall connection length must
not exceed 30m.
For Chromeleon versions 6.11, 6.20 and 6.30, the USB installation of the detector is via the
Master Setup from the current Service Pack CD (part no. 4580.0316). Insert the CD into the CD
drive of your Chromeleon server PC. Double-click Setup.exe in the root directory. Click Next to
have the Master Setup check which Chromeleon version is installed on your PC. Follow the on-
screen instructions as they appear to install the driver. If a version is found for which no driver is
available, the setup aborts.
Starting with Chromeleon 6.40, the USB installation of the detector is handled automatically
during the Chromeleon setup. The user is not required to take any other steps.
5.1.2 LAN Installation
Please note: Use the LAN connection under Windows NT 4.0 only. For any other
operating systems, a USB connection is recommended.
Connect the detector to the mains using the power cord shipped with the instrument. Please
observe the information about the facility requirements and the electrical connection (→ page 8).
Use the LAN cable from the standard accessories kit (page 49) to connect the detector to the
LAN port on the Chromeleon server PC.
To ensure reliable communication between the detector and the Chromeleon server with a
LAN connection, we recommend using a LAN connection that is independent of the office
LAN, i.e., an instrument LAN, between the detector and the Chromeleon server PC. This type
of connection requires that you install a separate 10 Mbit network interface card in the
Chromeleon server PC and assign unique IP addresses to all connected devices. For
information about how to install the separate network interface card in the Chromeleon server
PC, see page 19.
If your PC already contains a separate network interface card for operating Summit HPLC
devices, the detector can be connected to this card. For information about how to connect the
detector using an instrument LAN under Windows NT4, see below.
Important: Dionex cannot guarantee reliable communication when operating the

detector on an office LAN because the load of the office LAN is a decisive
factor. Overload of the office LAN may result in timeouts and loss of data,
and thus disturb automatic operation of Chromeleon. Therefore, we advise
against operating the detector on an office LAN.
18
Connecting the Detector via an instrument LAN under Windows NT4

If the Chromeleon server PC is running Windows NT 4.0, the detector must be connected to the
server PC via a LAN. NT 4 does not support USB.
If you wish to connect no other instrument but a single detector, you can connect the server PC
and the detector via a so-called crossover cable; a special network cable with crossed-over data
lines. These lines are usually marked by colors (red, yellow) to distinguish the cable from
standard network cables.
Thus, the structure of the instrument LAN is as follows (peer-to-peer connection):
Fig. 10: Peer-to-peer connection
If you wish to connect several TCP/IP-enabled Summit HPLC devices to the server PC, you
must use a hub (10 Mbit) to establish the connection to the instrument LAN. In this case, use
standard network cables instead of the crossover cable (One standard network cable, part no.
8906.2038, is provided in the accessories kit.)
Fig. 11: Hub connection
Separate Network Interface Card (NIC)

A network connection that is independent of the office LAN is called an instrument LAN. This
kind of connection between the detector and the Chromeleon server requires the installation of a
separate 10 Mbit network interface card (NIC, in the operating system = adapter) in the
Chromeleon server PC.
Install the card according to the manufacturer's instructions, via Start → Settings → Control
Panel → Network → Adapters → Add.
19
Fig. 12: Installing the separate network interface card
Configure the network adapter for the instrument LAN (here: [2] 3Com Fast EtherLink XL
NIC (3C905B-TX)) on the Bindings tab page. First, select all protocols in the Show
Bindings for field to verify that the TCP/IP protocol is installed. Install the TCP/IP protocol,
if necessary:
Fig. 13: Show bindings for all protocols
20
Then, select all adapters in the Show Bindings for field and disable all protocols for the
adapter for the instrument LAN except the TCP/IP protocol:
Fig. 14: Show bindings for all adapters
An IP address and a subnet mask are required for the separate network adapter because the
TCP/IP protocol is used for the instrument LAN. The dialog box for entering the IP address
and the subnet mask opens automatically during the installation.
Please note: You may as well open this dialog box later again to change the IP address
and the subnet mask if necessary. Select the properties of the TCP/IP
protocol via Start → Settings → Control Panel → Network →
Protocols. Then, select the TCP/IP protocol and open the Properties of
Microsoft TCP/IP dialog box, using the Properties command on the
context menu.
21
Fig. 15: Assigning the IP address and subnet mask
In the Adapter field, select the network adapter for the instrument LAN and enable the
Specify an IP address option.
Important: Do not change the settings for the office LAN network interface card!
Important: For the adapter for the instrument LAN, disable all other network
protocols and services (see above).
Assign a fixed IP address to the adapter for the instrument LAN.
First, identify which IP range is already used. To do so, open a DOS command box via
Start → Programs → Command Prompt and enter: Ipconfig /all. Differentiate between the
two following cases:
Case A: Already used IP address ≠ 192.168.60.xxx
Case B: Already used IP address = 192.168.60.xxx
Note: The subnet mask determines which part of the IP address describes the network
and which part defines the individual devices connected to the network.
Independent LANs have different network addresses. Based on the subnet mask
255.255.255.0 in the above example, the term "192.168.60" describes the
network, while "xxx" defines the individual modules connected to the network.
22
Case A:
Select the address from the range given below and enter the subnet mask specified below:
IP Address: 192.168.60.xxx (xxx can be any number between 1 and 254)

Subnet Mask: 255.255.255.0
Do not enter a gateway address; no gateway is available for the instrument LAN and the
above addresses cannot be routed. Communication between the detector and the Chromeleon
server is always direct
Case B:
If the range 192.168.60.xxx is already used, use a different network range for the instrument
LAN, e.g., use 192.168.61.
Important: In this case, make sure that the addresses assigned to the individual
Summit HPLC modules also start with 192.168.61.
For information about the appropriate value for your individual configuration, contact your
system administrator.
Do not enter a gateway address; no gateway is available for the instrument LAN and the
above addresses cannot be routed. Communication between the detector and the Chromeleon
server is always direct.
Applies to both, case A and case B

It may happen that the detector that you want to control via the separate 10Mbit network adapter
may already have an IP address and subnet mask assigned. In this case, when selecting the IP
address of the network adapter of the server PC, be sure that only the last digit differs from the IP
address of the detector. Otherwise, it may not be possible to address the connected Summit
HPLC devices.
Example for a valid configuration for case A:
Detector: IP address 192.168.60.51

Subnet mask 255.255.255.0
Server: IP address 192.168.60.50

Example for a valid configuration for case B:


23
Important: Every device on the same network must have a unique IP address.
Selecting duplicate IP addresses may cause conflicts on the network.
Important: Do not use 0 or 255 as the last numbers in IP addresses.
To check the selected settings, enter the following commands at the DOS level:
ipconfig /all
What it does: Lists the configuration of all network adapters. The newly installed 10Mbit
network adapter has been configured successfully it this command also returns its IP address
and subnet mask.
route print
What it does: Checks whether you can address the detector with the network adapter used for
the instrument LAN. The new network adapter is correctly configured if the indicated
network (here, for example, 192.168.60.0) is given under Target and its subnet mask is given
under Net mask.
ping 192.168.60.51
What it does: Checks the connection to the detector with the IP address 192.168.60.51. If the
installation is correct, the detector will reply to the ping command.
5.1.2.1 Assigning IP Properties to the Detector (CmIPUtil)
To operate the detector on a network, an IP address and a subnet mask must be assigned. The
program used to assign these IP properties is the CM IP Utility.
It is only possible to assign IP properties when data are not being transferred between the
detector and the Chromeleon server. To stop the Chromeleon server, select Start → Programs
→ Chromeleon → Server Monitor. In addition, the PC on which the CM IP Utility is running
must be connected to the same subnet as the detector you wish to program.
• If necessary, copy the CmIPUtil program from the software CD to a separate directory on
your computer.
• Double-click Setup.exe to start installation. Follow the installation instructions as they appear
on the screen.
• Open the program via Start → Programs → Chromeleon → IP Utility → CmIPUtil.
24
Fig. 16: Assigning IP properties (CmIPUtil)
The Module Information window of the IP Address Assignment dialog box lists all
modules that are active on the network, together with their IP address, serial number (S/N),
server name (the name or IP address of that server is given with which data are currently
exchanged), gateway address, subnet mask, and Ethernet address. The IP address for
modules that have not yet been configured is 0.0.0.0 or 255.255.255.255 (→ figure above).
• Enter the IP address and the subnet mask for the modules to be configured. The values depend
on the settings for the adapter for the instrument LAN (→ page 23).
• Select the module to be configured by its serial number.
• In the Available IP Addresses window, select the IP address to be assigned to the detector. If
it is not listed, click Add/Remove to enter the desired address.
• Enter the required subnet mask. Select the same subnet mask as used for the Chromeleon
server PC.
When selecting the IP address of the network adapter of the server PC, be sure that only the
last digit differs from the IP address of the detector. Otherwise, it may not be possible to
address the connected Summit HPLC devices.
Example for a valid configuration:


25
To configure several TCP/IP-enabled Summit HPLC devices, make sure that every device has
its own IP address. In this example, the following entries would be possible:
2. TCP/IP-enabled device: IP address 192.168.60.52

3. TCP/IP-enabled device: IP address 192.168.60.53

Important: Every device on the same network must have a unique IP address.
Selecting duplicate IP addresses may cause conflicts on the network.
Important: Do not use 0 or 255 as the last numbers in IP addresses.
• The Gateway Address field must not be empty, although no gateway is available for the
instrument LAN. Therefore, enter the IP address of the device to be configured in this field to
prevent a gateway from being used.
• When you finish entering IP properties, assign them to the module by clicking Assign. After
about 1 second, the instrument's new parameters will be displayed in the Module Information
window
• Click the Query button to check the settings.
• Click Exit to close the program.
Important: Verify that a unique IP address has been assigned to all Summit HPLC
devices that are connected to the Chromeleon server PC.
For more information, please refer to the CmIPUtil online Help.
5.1.2.2 Recommendations for Network Operation
Unlike USB connections, Ethernet LAN connections have limited real-time capabilities. Data
transmission can occur only when no other connected user is using the network. In order to
take full advantage of the real-time capabilities of the detector, select the instrument LAN
connection (→ page 19). This ensures that other network users do not delay the server
communication.
If the detector is connected to the data system via a LAN, it is not advisable to use the digital
inputs of the detector for remote inject synchronization. This is because the timing of LAN
communication varies, depending on the current LAN traffic.
If the detector is connected to the data system via USB, all remote inputs are polled after 0.1
seconds and they can be used without restrictions.
Please note: Dionex cannot guarantee reliable communication when operating the
detector on an office LAN because the load of the office LAN will be a
decisive factor. Overload of the office LAN may result in timeouts and
loss of data, and thus disturb automatic operation of the data system. We
therefore advise against operating the detector on an office LAN.
26
5.2 Installing the Detector in Chromeleon

Proceed as follows to install the detector in the Chromeleon installation program (= server
configuration):
• Start the Server Monitor program by selecting Server Monitor from the Start → Programs
→ Chromeleon menu on the taskbar. Start the server and close the Server Monitor window.
The Server Monitor icon appears on the task bar.
Please note: Clicking the Quit Monitor button quits (exits) the Server Monitor
program, but it does not stop the server. To stop the server, click the
Stop button.
• Start the Server Configuration program by selecting Server Configuration from the Start
→ Programs → Chromeleon menu on the taskbar.
• If necessary, click the "+" character beside the server name to display the items under the
server.
• Select the timebase to which to add the detector.
• Select Add Device… on the Edit or context menu. The Add device to timebase dialog box
is opened.
• Select Dionex from the left list box, and then select UVD 170U UV-VIS Detector or
UVD340U PDA detector from the right list box. (For Chromeleon < 6.50: Select either
Dionex UVD 170U UV-VIS Detector or Dionex UVD 340U PDA from the driver list.)
Click OK to confirm your selection.
The tabbed pages show the current configuration of your detector.
General Tab
Define the general instrument and hardware parameters:
Fig. 17: General tab page
27
• The Device Name field indicates the instrument name under which the detector is listed in the
installation environment (default: UV).
Please note: Do not change the default device name (UV). If the default is changed,
the links to the corresponding control panels may be lost.
• Click the Firmware Download button to download the current firmware version of the UVD
driver from the data system to the detector. The current firmware version as well as the
version of the file that is available in the \Bin directory of your Chromeleon installation
(UVD.HEX) is displayed.
Click OK to start the firmware download. Please note that the Chromeleon server needs to be
in idle mode for the download. The download takes several seconds.
Important: Wile the download is running, make sure that the communication
between Chromeleon and the detector is not interrupted and do not turn
off the detector. Otherwise, the entire firmware may be lost. If the
download is not successful, contact Dionex Service.
• Click the Browse… button to select the Module Address of the desired detector. This opens
a window that shows information about all available installed detectors (USB and LAN
connections). For example, if the driver for the UVD 170U detector is installed, all installed
UVD 170U detectors are displayed in the list.
Fig. 18: Device list
The Address column lists all available modules. The entry that is available via Ethernet
(TCP/IP) includes the module's IP address. The entry for a module that is available via
USB (identified by the prefix "USB") includes the module's Ethernet address or serial
number.
If a module listed here is already connected to a Chromeleon server, the server name appears
in the Server Name column. If a module is available for connection, the column shows
0.0.0.0 or is empty.
The S/N column lists the module’s serial number and the name of the computer to which the
module is connected while the Chromeleon server is running. The Ethernet column shows
the module’s Ethernet address.
Select the desired detector by double-clicking. The module's address is automatically entered
in the Module Address field on the General tab page.
28
• Verify that the Demo Mode is turned off. Select Off to disable the demo mode if necessary.
Select Read to read and display data from an existing demo file instead of real data. Click the
Demo File Name button to select a specific demo file.
Select Write to save the data currently delivered by the detector as a demo file. Click the
Demo File Name button to determine the directory and the file name.
Signals Tab
The Signals tab page indicates the names of all signals or channels that can be recorded via
the detector. In addition, it shows the signal unit (Unit) and the amplifying factor (Factor) of
each signal.
Fig. 19: Signals tab page
Select a check box to enable (5) or disable ( ) the corresponding signal or (device) channel.
Please note: Only activated signals will be available in Chromeleon. Raw data
acquisition is possible for active signals and/or channels only.
The following channels are available: UV_VIS_1, UV_VIS_2, UV_VIS_3, and UV_VIS_4.
To change the signal settings, select the required signal, and click Change:
Signal parameter Description

Signal Name Signal name
Unit Signal unit of the recorded raw data
Factor Currently set amplifying factor
DAC Channel DAC channel currently being used for conversion into an analog signal. This
channel can be selected only if a 16-bit DAC board is installed correctly.
DAC Factor Amplifying factor for the converted analog signal
29
Relays Tab
The Relays tab page lists all relays that are available for the detector. Make sure the
corresponding check box is selected (5). If it is not, the channel will not be available in
Chromeleon.
Option Description
RELAY170_1 UVD 170U only
Inputs Tab
The Inputs tab page lists the digital inputs that are available for the detector. Make sure the
corresponding check box is selected (5). If it is not, the channel will not be available in
Chromeleon.
Option Description
INPUT170_1 UVD 170U only
Error Levels Tab

The Error Levels tab page classifies the severity of any errors that occur. It is generally not
necessary to change the default settings.
Finally, save and close the modified Server Configuration.
Important: Before turning off the detector, always "disconnect" the unit in
Chromeleon.
For more information about how to configure the detector, refer to the Chromeleon online Help.
30
5.3 Operation with Chromeleon

Chromeleon can control the detector in two ways:
• Directly, via the icon and menu commands or via the controls in the UVD170U or UVD340U
control panel
• Via time-based programs (PGM-file or programmable buttons)
The following commands and properties are available in the Expert mode; some of them are
displayed only if the corresponding check boxes have been selected in the Server Configuration
program (for information about how to set the Expert mode, refer to the Chromeleon online
Help):
Command/Property Description
AcqOff Terminates data acquisition.
AcqOn Starts data acquisition.
Autozero Automatic null balance (= the current detector signal is interpreted as 0).
Average Averages all measured values over the Step interval. Default: ON. OFF records
only the last data point of each interval.
Bandwidth Sets the bandwidth of a UV-VIS channel
(Range 1 to 395 nm) (Also, see Wavelength).
BunchWidth For UVD 340U only: Averages photodiode signals for 3D field acquisition
(range 1.9 to 197.6 nm). Higher values for the bunch width enhance the signal–
to-noise ratio, but reduce the spectral resolution.
(Also, see MinWavelength, MaxWavelength)
CheckWavelength Performs a wavelength check, using a holmium oxide filter (→ page 33).
Connect Connects the detector to the data system. (Also, see Connected, Disconnect)
Connected Indicates whether the detector is connected to the data system, i.e., under
computer control. (Also, see Connect, Disconnect)
Delta Indicates the signal's slope, i.e., the difference between the current value and the
value one second ago; this is useful for triggers.
DemoFileName Name of the demo file (e.g., Demo.340).
Disconnect Disconnects the detector from the data system. (Also, see Connected, Connect)
FirmwareVersion Indicates the current firmware version (read-only).
HardwareVersion Indicates the hardware version of the detector (read-only).
Input 170_1 / Input 170_2 Indicates the state of the digital input (On or Off).
Input 340_1 / Input 340_2
Lamp Indicates the lamp's state. The Lamp On (Off) command turns the lamp on or
off.
Lamp Age Indicates the lamp age (in hours).
LampIgnition Indicates the number of lamp ignitions.
LampIntensity Indicates the lamp intensity at 254 nm in counts/second (Range: 1 to 2000000
counts/second). The value can be used as a reference value to establish any
decrease in the lamp intensity, if the lamp intensity was also measured when the
lamp was new.
MaxAutoStep Maximum step rate for Auto-Step Mode (UV-VIS channel)
(Range: 0.1 to 5.1 sec, default: 5.1 sec).
MaxWavelength For UVD 340U only: Maximum wavelength of the 3D field
(Range: 210.0 to 595.2 nm). (Also, see MinWavelength, BunchWidth).
MinLampIntensity At every AcqOn, the current lamp intensity is compared to the
MinLampIntensity. If the current lamp intensity is below the value entered here,
a warning is written to the Chromeleon audit trail.
31
Command/Property Description
MinWavelength For UVD 340U only: Minimum wavelength of the 3D field
(Range 200.0 to 585.2 nm). (Also, see MaxWavelength, BunchWidth)
ModelNo Indicates the detector type (e.g., UVD 340U; read-only).
RefBandwidth Sets the reference bandwidth
(3D field: range: 1.9 to 600.9 nm; UV_VIS: range 1 to 400 nm).
(Also, see RefWavelength, Bandwidth)
RefWavelength Sets the reference wavelength
(3D field: range: 200.0 to 600.9 nm; UV_VIS: range 200 to 600 nm).
The absorbency measured at this wavelength serves as a reference when
correcting the baseline (of a 3D field or a channel).
Relay170_1 / Relay170_2 The following commands are available: State (indicates or sets the state of the
Relay340_1 / Relay340_2 relay), Duration (when set, the relay's state toggles after the specified time), On
(turns the relay on), and Off (turns the relay off).
Retention Indicates the signal's retention time (in minutes).
SerialNo Indicates the serial number of the detector.
Signal Indicates the current signal value (UV-VIS channel). Available are: Value
(current signal value), UpperLimit, and LowerLimit. If the current signal value
is outside these limits, a warning is written in the Audit Trail.
SnapshotClearBLSpectrum For UVD 340U only: Clears the buffer for baseline spectrum that was recorded
using the SnapshotGetBLSpectrum command.
SnapshotDirPath For UVD 340U only: Defines the destination directory for a UV spectrum that is
recorded using the SnapshotGetSpectrum command.
SnapshotGetBLSpectrum For UVD 340U only: Saves the current spectrum as baseline spectrum. The
baseline spectrum is always subtracted from the spectrum recorded with the
SnapshotGetSpectrum command.
SnapshotGetSpectrum For UVD 340U only: Stores the current spectrum in a text file.
SnapshotResolution For UVD 340U only: Defines the wavelength resolution for a UV spectrum that
is recorded using the SnapshotGetSpectrum command
(Range: 0.1 to 20.0 nm).
Step Sets the step for data acquisition
(UV_VIS: range: 0.01 to 4.80 sec., AUTO. AUTO selects the best step
dynamically: 3D field: range: 0.1 to 4.0 sec).
Wavelength Sets the wavelength of a UV-VIS channel at which the channel measures the
signal (Range: 200 to 595 nm). (Also, see Bandwidth).
For information about individual commands and parameters and about the command syntax,
refer to the corresponding Chromeleon control panel. Select Command... on the Control
menu. The Commands dialog window is opened. You can also open this window by pressing
the F8 key. In the left list box, click the "+" character beside UV to display the items
underneath.
For more information (also on any parameter or command that might not be listed here), refer
to the Chromeleon online Help and/or Chromeleon manual.
32
5.4 Wavelength Calibration under Chromeleon

The wavelength is calibrated automatically after each "Lamp on" or "Connect" command
("detector calibration"). The "CheckWavelength" command checks the currently valid
wavelength calibration. The maximum deviation of this calibration compared to the
instrument status is given in the Audit Trail.
• During calibration, the baseline must be sufficiently stable. This may not be the case, e.g., if
the solvent composition has been modified or if there are bubbles in the solvent.
• The solvent in the cell must not be fully absorbing in the wavelength range to be calibrated.
This will be the case, e.g., if the cell is filled with 96% hexane/4 % ethyl acetate.
• Before calibration, make sure that the deuterium lamp is already warm because its spectrum
changes a lot during the first minutes after turning on the lamp.
If these conditions are not met, the process will be interrupted with the corresponding error
message. When the problem is solved, repeat the calibration using the Disconnect command
followed by the Connect command.
Calibrating the detector is via a holmium oxide filter that is installed in the beam path of the
deuterium lamp. The maxima are determined from the resulting transmission spectrum and
compared to the holmium oxide values stated in the literature. If a difference is detected for this
maximum between the measured value and the known value, interpolation is performed between
this maximum and the two adjacent maxima to correct the wavelength allocation of the affected
photodiodes. Wavelength calibration can take up to 2 minutes. During this time, data acquisition
is not possible.
5.5 System Wellness and Reliability

To help you detect small problems before they become big ones, Chromeleon supports
different System Wellness and reliability commands and properties, such as LampAge,
LampIgnitions, and MinLampIntensity. Also, see the corresponding commands in Operation
with Chromeleon (→ page 31).
If an error is found, an error message is displayed and the error is logged in the Chromeleon audit
trail.
For more information, please refer to the Chromeleon online Help.
33
34
6 Troubleshooting
6.1 The Most Frequently Problems
The following table provides a summary of the most commonly observed operating problems,
lists possible causes, and suggests remedial actions:
Problem Probable Cause Remedial Action

No function The detector is not connected to the Connect the power cord to its
mains. source.
The detector is not turned on. Turn on the detector.
The fuses blow. Replace the fuses
(→ section 3.2.2, page 10).
Replacement fuses blow Check the voltage selector setting;
immediately. reset the selector, if necessary
(→ page 8).
Call Dionex Service.
The USB or LAN cable between the Check the USB or LAN
detector and PC is not correctly connection to the PC.
installed.
The lamp is not turned on. The lamp is still too hot Wait 5 minutes, and then turn on
(message in Chromeleon: "Lamp the lamp again.
too hot...").
The lamp cannot be turned on Wait 5 minutes, and then turn on
(message in Chromeleon: "Lamp the lamp again. If several attempts
On failed..."). fail, check the lamp and the
connection cable.
The USB or LAN cable between the Check the USB or LAN
detector and PC is not correctly connection to the PC.
installed.
There is no signal or an The flow cell is contaminated. Clean the flow cell
irregular signal. (→ section 7.3.3, page 43).
Transmission through the mobile Select a different wavelength.
phase is low.
Check the solvent for
contaminants.
The spectral range is incorrect. Select a different spectral range.
The injection valve is faulty. Check the fluidics.
There is a fault in the diode array. Call Dionex Service.
The lamp intensity is too low. Replace the lamp
(→ section 7.2, page 41).
Strong baseline drift The warm-up time is too short. Allow min. 30 min. warm-up.
The column is contaminated. Rinse or replace the column.
The system is not equilibrated. Continue to rinse the system until
it is equilibrated.
The environment is unstable. Ensure constant temperature and
humidity.
The solvent is contaminated. Change the solvent.
The flow cell is contaminated. Clean the flow cell
(→section 7.3.3, page 43).
The lamp is defective. Replace the lamp
The solvent is used in a cycle Direct the solvent to the waste.
35

High noise level The solvent is contaminated. Replace the solvent.
The lamp is old. Replace the lamp
There are gas bubbles in the system. Prime the system.
The pump suffers from pressure Check and prime the pump.
pulsation.
The wavelength is incorrect. Select a suitable wavelength.
The step is too small (e.g., 0.1 sec.). Increase the step (e.g., to 0.5 to 1
sec.).
Peak broadening The capillaries are too long or their Shorten or replace with narrower
internal diameter is too large. capillaries.
The column is overloaded, Rinse or replace the column.
contaminated, or old.
The solvent is old. Replace with fresh solvent.
The aAnalysis is not The sample is unstable. Use fresh sample or change
reproducible. conditions.
The gradient cannot be reproduced. Change the gradient program
and/or check the pump.
The environment is unstable. Ensure constant temperature and
humidity.
The column is overloaded, Rinse or replace the column.
contaminated, or old.
There are air bubbles in the flow Prime the system.
cell.
36
6.2 The Most Frequent Error Messages

If communication between the data system and the column compartment cannot be
established, an error message is displayed in the Chromeleon Audit Trail. The following table
lists the most important error messages, along with possible causes and the corresponding
remedial actions.
Important: If the server configuration is changed in any way, messages referring to

the previous configuration may continue to appear for up to 30 seconds
after the change. These messages can be ignored. Thus, always note the
device name (UVD 340U@...) referenced in the message.
Please note: The error messages below may also read either UVD340U@ + serial
number (= UVD@USB-01610103) or UVD340U@ + IP address
(= UVD340U@192.168.50.1), depending on the connection type (USB or
LAN) specified for the column compartment in the Server Configuration.
The messages for the UVD 170U detector are identical to these except for
the detector name.

Error opening UVD 340U - Another Chromeleon server already Check that the correct detector was
Connection refused. uses the indicated detector. selected or terminate the
detector's communication with the
other Chromeleon server.
The existing connection is not yet Wait for 30 seconds and repeat the
cleared completely. procedure.
The subnet mask and gateway address Via CmIPUtil, make sure that the
of the detector are configured correct parameters are specified
incorrectly. (→ sec. 5.1.2.1, page 24).
A gateway may maintain an unintended Restart the detector.
connection to the detector as soon as
the Chromeleon server is stopped in an
uncontrolled way (power-failure,
system hung-up).
Error opening UVD 340U - The Chromeleon server failed to Check that the detector is turned
Connection timed out. connect to the indicated detector. on and that the network connection is
working.
Note the name of the indicated
detector. It is possible that the
Chromeleon server is trying to connect
to a detector that is no longer part of
the server configuration. Check and
change the Module Address, if
necessary. Then, retry.
The network connection is interrupted Check the network connection.

during firmware download.
The detector power supply is Check the electrical connection of the
interrupted during firmware download. detector.
UVD 340U - Connection reset by The power supply to the detector is Check the electrical connection of the
peer. interrupted for a short period. detector.
37

UVD 340U - Host not found. Network problems: no connection to Check the network settings for the
the detector. detector and network card. The
UVD 340U - Permission denied. network connection between the
Chromeleon server and the detector
UVD 340U - Network is
unreachable. might be inappropriate (→ sec. 5.1.2.2,
page 26) (contact your network
UVD 340U - No route to host. administrator).
UVD 340U @ USB-00-E0-EF-DE- The USB connection between the Check the USB connection.
XX-XX - Device not found on the detector and the Chromeleon server is
USB. interrupted.
The power supply to the detector is Check the electrical connection of the
interrupted. detector.
Error opening UVD 340U @ USB- The USB connection between the Check the USB connection.
00-E0-EF-DE-XX-XX – The detector and the Chromeleon server is
System cannot find the file specified interrupted.
The power supply to the detector is Check the electrial connection of the
Error issuing control request to The Chromeleon server cannot connect Check the USB and LAN connection
UVD 340U to the specified detector. respectively.
Check the electrical connection of the
detector.
Remove the detector specified in the
message from the server configuration
or else select a different detector from
the list of available detectors in the
Server Configuration program (via
Properties/Browse).
Error reading from UVD 340U @ There is a transmission error between Check the USB connection. The
USB-00-E0-EF-DE-XX-XX Data the detector and the Chromeleon connection length must not exceed 5 m
error (cyclic redundancy check) server. to the next hub. The overall connection
length including the hub connections
must not exceed 30 m (→ page 18).
Replace any defective USB cable or
hubs.
Error reading from UVD 340U @ The connection between the detector Check the USB connection.
USB-00-E0-EF-DE-XX-XX and the Chromeleon server is
interrupted.
The power supply to the detector is Check the electrical connection of the
Chromeleon Error Messages
For a list of all Chromeleon error messages, see the Chromeleon installation program (Server
Configuration). Double-click the detector in the left window section and select the Error
Levels tab (→ page 30) in the dialog box. You can also open the dialog box by clicking the
detector in the left pane and then selecting the unit's Properties on either the Edit menu or
the context menu.
38
7 Maintenance
7.1 General Information
The detectors are made of high-quality components and materials, which keep maintenance
requirements to a minimum. In general, the unit should be kept clean. The painted surfaces
are relatively resistant to weak acids and alkaline or organic solutions. Nevertheless,
immediately wipe up all liquids spilled onto the detector's painted surface, using a lint-free
cloth or paper.
The following sections describe all maintenance procedures that can be carried out by the user.
Only qualified Dionex personnel should perform any additional servicing.
Important: Always use the original packaging when shipping the detector. Shipping
the instrument in anything other than the original packaging will void the
product warranty! (→ Warranty, page 2).
All parts serviceable by the user (e.g., the lamp and flow cell) are accessible via the removable
cover on the detector's left-side panel. To open, press in and turn the knurled screw 90°
counterclockwise. You may now open and remove the panel. The lamp and flow cell are clearly
visible (→ Fig. 20).
Warning: During operation, the lamp becomes extremely hot and remains so for
some time after the unit is turned off. To avoid possible injury, allow
sufficient time for the lamp to cool before performing any maintenance.
39
Fig. 20: Side View (side panel open)
40
7.2 Deuterium Lamp
7.2.1 Removing the Lamp
sufficient time for the lamp to cool before performing any maintenance.
• If the detector is connected to Chromeleon, select the Disconnect command in Chromeleon to

terminate communication.
• Turn off the power to the detector and disconnect the unit from the mains.
• Remove the removable cover from detector's left-side panel. To open, press in and turn the
knurled screw 90° counterclockwise. You may now open and remove the panel. Do not bend
the Teflon capillary.
• Wait until the lamp has cooled down!
• Disconnect the lamp by gently pressing either side of the locking plug.
• Undo and remove the two lamp retaining screws (using a 2.5 mm Allen-key) and carefully
remove the lamp.
7.2.2 Installing the Lamp
• Insert the new lamp (part no. 5053.1204), aligning the notch on its base with the alignment
pin of the lamp housing and replace the retaining screws. No additional alignment is required.
Important: Avoid touching the glass tube of the lamp.
• Reconnect the cable.

• Reinstall the removable cover on detector's left-side panel.
• You may now turn on the detector and restore communication with Chromeleon, using the
Connect command.
Please note: A new lamp must "run in" for at least 24 hours before the first analysis.
During this period, strong baseline fluctuations and increased noise may
occur.
41
7.3 Flow Cell
Fig. 21: Standard flow cell
No. Description Part no.

1 M3x12 screw (DIN965) Included in 5065.1815
2 Heat exchanger Included in 5065.1815
3 PEEK tubing (0.5 mm ID) 2251.6002
4 PEEK fitting screw (1/16", 15 mm) Included in 5065.1815
5 M3x23 screw (DIN 965) Included in 6065.1815
6 Lens retaining plate 1 Included in 6065.1800
7 Quartz lens 1343.0511
8 Flow cell body -------
9 Lens retaining plate 2 Included in 6065.1800
10 Flow cell housing -------
11 Single-part hand-tight fitting Included in 5065.1815
12 Capillary tube (1.58 x 0.25 ID) Included in 5065.1815
13 PEEK double ferrule -------
14 Flow cell body retaining screw -------
15 Fitting screw -------
Part no. Description

5065.1815 Heat exchanger for analytical flow cell, including:
Heat exchanger
PEEK fitting screw (1/16", 15 mm)
Capillary tube (1.58 x 0.25 ID)
Single-part hand-tight fitting
M3x12 screw (DIN 965) (2 off).
6065.1800 Analytical flow cell repair kit, including:
M3x23 screw (DIN 965) (3 off),
Quartz lens (2 off.)
Lens retaining plate 1 (1 off)
Lens retaining plate 2 (1 off)
42
7.3.1 Removing the Flow Cell
sufficient time for the lamp to cool before performing maintenance.
• If the detector is connected to Chromeleon, first terminate communication by selecting the

Disconnect command in Chromeleon.
• Turn off the detector. Disconnect the power cord.
• Remove the removable cover from detector's left-side panel. To open, press in and turn the
knurled screw 90° counterclockwise. You may now open and remove the panel. Do not bend
the Teflon capillary.
• Undo the four retaining screws and remove the flow cell cover.
• Undo the knurled flow cell retaining screw and carefully remove the flow cell assembly.
7.3.2 Installing the Flow Cell
Important: Do not use the UVD 160S/320S flow cells in the detector.
• Insert the new or cleaned flow cell, aligning the notch with the alignment pin of the flow cell
socket. No further alignment is necessary.
• Reinstall the flow cell cover.
• Reinstall the removable cover on detector's left-side panel.
• You may now turn on the detector and restore communication with Chromeleon, using the
Connect command.
7.3.3 Cleaning the Flow Cell
Important: Unlike the other flow cells, it is not possible to disassemble the fused silica
nano flow cell for cleaning. Clean the nano flow cell by flushing with
0.2M nitric acid, followed by water and methanol.
Important: When cleaning the flow cell, note that only the lens retaining plates and
the lenses may be removed. Do not attempt to remove the flow cell body
from its housing! Clean the flow cell body together with the housing.
To clean the flow cell (except the nano flow cell), follow the steps below. The numbers in
parentheses refer to the corresponding parts in Fig. 21:
• Undo and remove the screws (1) and remove the heat exchanger (2).
• Undo and remove the screws (5) and carefully remove the lens retaining plates (6 and 9) and
lenses (7).
• To avoid scratching the lenses, place them on a clean, lint-free cloth or tissue.
43
• Clean the lenses, using a soft, lint-free cloth or tissue and an optical cleaning solution or
isopropanol.
• Replace scratched or damaged lenses.
Important: Do not attempt to remove the flow cell body (8) from the housing (10)!
• To clean the flow cell body and housing, rinse the parts with water, isopropanol, or methanol,
or place the entire assembly in an ultrasonic bath.
Important: Never attempt to remove dirt from the flow cell body using a spatula,
tweezers, or other object, as this may cause irreparable damage.
• Reinstall the lenses onto either side of the flow cell body so that the plane faces are on the
inside.
• Reinstall the lens retaining plates (6 and 9), taking care to tighten the screws (5) evenly and
without excessive force.
Important: If you have loosened the fitting screws (4 and/or 15) on the flow cell body,
take care not the re-tighten the screws too tight to prevent damage to the
flow cell body.
• Check the flow cell for leakage before you install them into the unit. Tighten the screws (5) if
necessary.
44
8 Upgrade Options
The table below lists all upgrade options available for the UVD 170 and 340 detectors. If you
have any questions, please contact Dionex Sales or your Distributor.
Part No. Description

5071.0050 Upgrade kit UVD 170U to UVD 340U
5071.0060 Upgrade kit UVD 170S to UVD 340U
45
46
9 Technical Specifications
Performance
Wavelength range Programmable
200 nm - 344 nm (UV range)
345 nm - 595 nm (VIS range)
Noise < ± 5µAU
(wavelength: 254 nm, bandwidth: 8 nm, dry flow cell)
Drift < 5 x 10-4AU/h, after 3h warm-up at a wavelength of
254 nm and with a dry flow cell
Wavelength accuracy ± 0.75 nm (UV range)
± 1.5 nm (VIS range)
Spectral resolution UVD 340U: 1.9 nm
Bandwidth Programmable from 1.9 to 400 nm
Linearity ≤ 1% at 1.5 AU
Measuring range 0 to 2AU
Time constant 0.1 to 4.0 s, programmable
Power-up wavelength Automatic via internal holmium-oxide filter
verification
Wavelength calibration Automatic or on demand
Minimum step 0.1 s
Optical System
Principle Single-beam principle with simultaneous measurement
across the photodiode array.
Optical bench Photodiode array bench without moving parts
Pixel resolution 1.9 nm (UV range)
3.3 nm (VIS range)
Light source Deuterium lamp
Electronics
Control All functions controllable via LAN or USB
Program-controlled by Chromeleon
permanent status transfer via 2 LEDs on the front panel
PC connection Via USB or Ethernet-LAN (RJ45, 10 MBit) connection
Additional USB ports Integrated USB hub with three type A USB ports for
connection of other Summit HPLC modules
I/O interfaces Digital I/O:
2 inputs (digital input voltage: 0-5V),
2 relay outputs (default open), can also be used as TTL
compatible inputs.
47
Signal output UVD 170U: 4 detection channels

UVD 340U: 4 detection channels plus 3D-field
(with Chromeleon PDA license)
Flow Cells
Standard analytical Volume: 10 µL
flow cell: Path length: 9 mm
Max. Pressure limit: 100 MPa (1450 psi)
Biocompatible flow cell Volume: 10 µL
- optional - Path length: 9 mm
Semi-preparative flow cell Volume: 6 µL
Micro flow cell Volume: 2.5 µL
Fused silica nano flow cell Volume: 395 nL
Physical Specification
Power requirements 115/230V ± 10%, 50/60Hz
Power consumption 50VA
Environmental conditions: Range of use: Indoor use
Temperature: 10°C to 35°C (50°F to 95°F)
Air humidity: 40 to 85% relative humidity,
non-condensing
Overvoltage category: II
Pollution degree: 2
Instrument panel 1 two-colored LED (red/green) indicating the instrument
status (connection to Chromeleon/deuterium lamp)
Wetted parts PEEK, quartz, stainless steel
Dimensions 210 x 480 x 430 mm
(w x h x d) 8.3 x 18.9 x 16.9 in
Weight 20kg (44 lbs)
Technical information: May 2003.
All technical specifications are subject to change without notice.
48
10 Accessories and Spare Parts

Accessories and spare parts are always maintained at the latest technical standard. Therefore,
part numbers are subject to change. However, updated parts are always compatible with the
parts they replace.
10.1 Standard Accessories (included in the shipment)

The following standard accessories (part no. 5071.9000) are shipped with the detector.
Accessory parts listed below without their part numbers are usually available in one of the
UVD-U spare parts kits (→ section 10.2, page 50).
Part No. Description Qty*

1A fuse, slow, 5x20 mm 2
(available as spare part in the UVD Fuses kit, part no. 6065.9002)
2A fuse, slow, 5x20 mm 2
(available as spare part in the UVD Fuses kit, part no. 6065.9002)
1310.7031 or Power cable (220V) or 1
1310.7032 Power cable (125V) or
(depending on the destination country)
2146.1051 Wrench 1/4" x 5/16" 1
2146.2625 Pin spanner 2,5 1
2261.0121 PEEK fitting screw 1/16", 15mm 2
2261.0122 PEEK double ferrule 2
2309.1100 Assortment box for accessories 1
6007.9100 Silicone tube (2.8 x 1.3), 3m 1
8906.2038 RJ45 cable for Ethernet connection, 3m 1
8911.0001 Mini-DIN signal cable, 6-pin, 5m 1
8911.0002 USB cable, 5m, Type A to Type B 1
4580.0316 Chromeleon 6 Service Pack CD 1
4820.7100 or UVD 170/340U Operating Instructions, German or 1
4820.7150 UVD 170/340U Operating Instructions, English
(depending on the destination country)
* The part number always refers to the packing unit. For further information, please contact your Dionex sales representative.
49
10.2 Optional Accessories and Spare Parts
Part no.* Description

1343.0511 Quartz lens for standard flow cell
2101.0303 M3 knurled flow cell retaining screw
2200.5502 Single-part, hand-tight fitting
2250.1001 Capillary (1.58x0.25 ID)
2251.6002 PEEK capillary tube for standard flow cell (1/16“, 0.50 mm)
2261.0121 PEEK fitting screw (1/16“, 15 mm)
2261.0122 PEEK double ferrule for standard flow cell
3321.0000 10 Standards (Uracil/Pyrene in MeOH)
(Required for the General Function Test during Installation Qualification)
5053.1204 Deuterium lamp
5065.1800 Semi-preparative flow cell (6 µL)
5065.1810 Standard analytical flow cell (10 µL)
5065.1810M Micro flow cell (2.5 µL)
5065.1810N Fused silica nano flow cell (395 nL)
5065.1815 Heat exchanger for analytical flow cell, including:
Heat exchanger
PEEK fitting screw (1/16", 15mm)
Capillary tube (1.58x0.25 ID)
Single-part, hand-tight fitting
M3x12 screw DIN965 (2 pcs).
5065.1816 Heat exchanger for preparative flow cell, including:
Heat exchanger
PEEK fitting screw (1/16", 15mm)
Capillary tube (1.58x0.70 ID)
Single-part hand-tight fitting
Screw M3x12 DIN965 (2 pcs).
5065.1820 Biocompatible flow cell (10 µL, PEEK capillaries)
6065.1800 Repair kit for analytical flow cells, including:
M3x23 screw DIN 965 (3 pcs.),
Quartz lens (2 pcs.)
Lens retaining plate 1 (1 pc.)
Lens retaining plate 2 (1 pc.)
6065.9001 UVD 340 / 170 Repair Kit
Quartz lenses (2 pcs)
PEEK capillary tubing (1/16", 0.50 mm, 2 m)
PEEK fitting screws (1/16", 15 mm) (2 pcs)
PEEK double ferrule (2 pcs)
Deuterium lamp (2 pcs)
50
Part no.* Description

6065.9002 UVD Fuses kit, including:
0.80A fuse, medium-slow, 5x20mm (1 pc)
1A overload fuse, slow, 5x20mm, (2 pcs)
2A overload fuse, slow, 5x20mm (2 pcs)
8911.0004 USB extension with signal amplifier, 5m
* The part number always refers to the packing unit. For further information, please contact your Dionex sales representative.
51
52
11 Index
A F
Accessories .......................................................... 49 FCC Note ............................................................... 3
Optional........................................................... 50 Firmware Download ............................................ 28
Standard........................................................... 49 Flow Cell ......................................................... 6, 42
AcqOff ................................................................. 31 Biocompatible ............................................. 6, 48
AcqOn.................................................................. 31 Cleaning .......................................................... 43
Autozero............................................................... 31 Fused silica nano flow cell .............................. 48
Average ................................................................ 31 Fused Silica Nano Flow Cell............................. 6
Installation....................................................... 43
B Micro Flow Cell .......................................... 6, 48
Removal .......................................................... 43
Bandwidth ...................................................... 31, 47 Semi-Preparative ......................................... 6, 48
BunchWidth ......................................................... 31 Standard (Analytical) .................................. 6, 48
Fuse Replacement ................................................ 10
C
Capillary Connections.......................................... 13 I
CheckWavelength .......................................... 31, 33 Input..................................................................... 31
Chromeleon Installation ............................................................. 7
Commands....................................................... 31 Environment...................................................... 7
Installation....................................................... 27 LAN .......................................................... 18, 19
Operation......................................................... 17 Mains Connection ............................................. 8
Wavelength Calibration................................... 33 Network Interface Card (NIC) ........................ 19
Error Messages................................................ 38 Positioning ........................................................ 7
Cleaning (Flow Cell)............................................ 43 Rear Panel Connections .................................. 11
CmIPUtil .............................................................. 24 under Chromeleon........................................... 17
Configuration USB................................................................. 17
for Network Operation .................................... 24 Intended Use .......................................................... 3
Separate Network Interface Card .................... 19 Interfaces.............................................................. 47
Connect ................................................................ 31
Connected ............................................................ 31
L
Connections
Capillaries ....................................................... 13 Lamp .............................................................. 31, 47
Digital I/O ....................................................... 12 Installation....................................................... 41
LAN ................................................................ 12 Removal .......................................................... 41
Rear Panel ....................................................... 11 LampAge ............................................................. 31
USB................................................................. 11 LampIgnition ....................................................... 31
Control ................................................................. 47 LampIntensity ...................................................... 31
LAN ..........................................................12, 18, 19
D LEDs .................................................................... 48
Linearity............................................................... 47
Data Channels ...................................................... 48
Delta..................................................................... 31
M
Demo Mode ......................................................... 28
DemoFileName .................................................... 31 Mains Connection .................................................. 8
Digital I/O ............................................................ 12 Maintenance......................................................... 39
Dimensions .......................................................... 48 Manual ................................................................... 1
Disconnect............................................................ 31 MaxAutoStep ....................................................... 31
Drift...................................................................... 47 MaxWavelength................................................... 31
Measuring Range ................................................. 47
E MinLampIntensity ............................................... 31
MinWavelength ................................................... 32
Environmental Conditions ................................... 48 ModelNo .............................................................. 32
Error Levels (Tab Dialog Box) ............................ 30
Error Messages..................................................... 37
53
SnapshotDirPath .................................................. 32
N SnapshotGetBLSpectrum..................................... 32
SnapshotGetSpectrum.......................................... 32
Network Interface Card (NIC) ............................. 19 SnapshotResolution ............................................. 32
Network Operation (Configuration)..................... 24 Spare Parts ..................................................... 49, 50
Recommendations ........................................... 26 Specification ........................................................ 47
Noise .................................................................... 47 Spectral Resolution .............................................. 47
Status LED........................................................... 15
O Step ................................................................ 32, 47
System Wellness .................................................. 33
Operation.............................................................. 15
after Power-Failure.......................................... 15
Chromeleon ..................................................... 31 T
Power-Up ........................................................ 15 Tab
Principle ............................................................ 5 Error Levels..................................................... 30
Status LED ...................................................... 15 General............................................................ 27
Optical Bench....................................................... 47 Inputs............................................................... 30
Optical System ..................................................... 47 Relays.............................................................. 30
Overview................................................................ 5 Signals............................................................. 29
Technical Specification........................................ 47
P Time Constant...................................................... 47
Troubleshooting ................................................... 35
PC Connection ..................................................... 47
Error Messages................................................ 37
Peer-to-Peer Connection ...................................... 19
Problems ......................................................... 35
Pixel Resolution ................................................... 47
Power Consumption............................................. 48
Power Requirements ............................................ 48 U
Power-Failure....................................................... 15 Unpacking.............................................................. 2
Power-Up ............................................................. 15 Upgrade Options .................................................. 45
Problems .............................................................. 35 USB................................................................ 11, 17
R V
Rear Panel Connections ....................................... 11 Voltage Selector..................................................... 8
RefBandwidth ...................................................... 32
RefWavelength .................................................... 32
W
Relay .................................................................... 32
Reliability............................................................. 33 Warranty ................................................................ 2
Replacement (Fuse).............................................. 10 Wavelength .......................................................... 32
Resolution ............................................................ 47 Accuracy ......................................................... 47
Retention .............................................................. 32 Calibration....................................................... 47
Range .............................................................. 47
S Verification ..................................................... 47
Wavelength Calibration ....................................... 33
Scope of Delivery .................................................. 2 Weight.................................................................. 48
SerialNo ............................................................... 32 Wellness............................................................... 33
Signal ................................................................... 32 Wetted Parts......................................................... 48
Signal Output ....................................................... 48
SnapshotClearBLSpectrum .................................. 32
54

UVD 170U and UVD 340U UV/VIS Detectors: Operating Instructions

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UVD 170U and UVD 340U

• Low-Voltage Equipment Directive 73/23/EEC

This declaration is issued for the manufacturer

by the President, Dr. Peter Jochum.

5 Automated Control by Chromeleon .............................................................................. 17

7.3 Flow Cell..................................................................................................................... 42

9 Technical Specifications .................................................................................................. 47

10 Accessories and Spare Parts........................................................................................ 49

Please note: Indicates general information to optimize the performance of the

The information contained in this document is subject to change without notice.

To unpack the detector, proceed as follows:

The warranty coverage does not include the flow cell.

1.4 Intended Use

If there is a question regarding appropriate usage, contact Dionex before proceeding.

1.5 Federal Communications Commission (FCC) Note

Please note: Recording of three-dimensional data is only possible with the

• Maximum analysis efficiency, due to comprehensive data analysis capabilities in

Fig. 1: Principle of operation

2.2 Flow Cells

Fig. 2: Summit HPLC system with detector

3.2 Electrical Connection

3.2.1 Setting the Voltage Selector

Fig. 3: Factory-set voltage selection, here: 230V

• Remove the fuse cartridge using a small screwdriver (→ Fig. 4).

Fig. 4: Fuse cartridge

Fig. 5: Voltage selector board

Please note: Set the voltage selector as follows:

• Reinstall the voltage selector board in the power socket housing.

3.2.2 Replacing the Fuses

To replace the fuses, follow the steps below:

• Remove the fuse cartridge, using a small screwdriver (→ Fig. 4).

Voltage Description Part-Nr.

• Reinstall the fuse cartridge.

3.3 Rear Panel Connections

The Chromeleon Chromatography Management

Data transfer is digitally via the respective cables

Important: To ensure trouble-free operation,

For more information about the single ports, see below.

Fig. 8: Rear panel view

Fig. 9:Digital I/O port (View from the rear)

3.4 Fluid Connections

Important: The flow cell is primed with 2-propanol.

4.2 Front Panel Status LED

4.3 Operation after Power Failure

5 Automated Control by Chromeleon

• For Chromeleon ≤ 6.40:

5.1.1 USB Installation

5.1.2 LAN Installation

Important: Dionex cannot guarantee reliable communication when operating the

Connecting the Detector via an instrument LAN under Windows NT4

Thus, the structure of the instrument LAN is as follows (peer-to-peer connection):

Fig. 10: Peer-to-peer connection

Fig. 11: Hub connection

Separate Network Interface Card (NIC)

Fig. 12: Installing the separate network interface card

Fig. 13: Show bindings for all protocols

Fig. 14: Show bindings for all adapters

Fig. 15: Assigning the IP address and subnet mask

Assign a fixed IP address to the adapter for the instrument LAN.