Bioinstrumentation III Sem
Bioinstrumentation III Sem
Bioinstrumentation III Sem
SEMESTER - III
MAJOR-PRACTICAL
BIOINSTRUMENTATION
OBJECTIVES :
CO1. To enable Students to understand about Analytical Techniques & to know about Principles and Mechanism
of Bioinstrumentation, CO2. To enable Students to describe current evidence regarding contributing factors that
affect balance control, CO3. To identify a balance-specific outcome measure and be able to describe the
reliability, validity, and clinical usefulness, CO4. To provide the Knowledge of pH great many situations, including
HOURS / WEEK - 8
of course chemical laboratory, CO5. To enable Students to explain the principles of a wide range of modern
analytical methods of separation and quantitation, CO6. To evaluate strengths and limitations of different
HOURS / SEMESTER - 120
separation and detection techniques with respect to sample properties and to specific analytical CO-CURRICULA
problems.
CIA LECTURE
R ACTIVITIES
16 8 96
SEMESTER - III
MAJOR-PRACTICAL
BIOINSTRUMENTATION
Nephelometry & Turbidometry - Theory, Instrumentation, Applications
UNIT VI (LECTURE HOURS: 48)
BIOINSTRUMENTATAION LAB
BIOINSTRUMENTATION PRACTICAL EXPERIMENTS - 1. Acid-Base Titration curves & pKa Value Determination 2.
Preparation of Buffers 3. Sterlization - Aim, Dry heat ,Moist heat, Gaseous chemicals, Filtration & Radiation, Result &
Discussion 4. Pure Culture Technique 5. Separation of lipids by Thin layer Chromatography- Aim, Principle, Procedure
& Result & Discussion. 6. Separation of plant pigments by Column Chromatography - Aim, Principle, Procedure &
Result & Discussion. 7. Preparation of SDS - PAGE -Aim, Principle, Procedure, Demonstration & Result & Discussion.
8. Preparation of Agarose gel electrophoresis- Aim, Principle, Procedure & Result & Discussion. 9. Estimation of
proteins by Bradford method 10. Estimation of DNA by Diphenylamine Method
Text Books :
Bioanalytical techniques | Edition:1 | IK International Publishing House Pvt Ltd | Sekhar Talluri(2009)
Bioinstrumentation | Edition:1 | MJP Publishers, | L Veerakumari (2015)
Reference Books :
Experimental Procedure in life sciences | Edition:1 | Anjanaa Book house | Dr. S. rajan AND R. Selvi Christy(2015)
Practical Biochemistry | Edition:3 | Tata McGraw Hill | David Plummer(2002)