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WO2010002160A3 - 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도 - Google Patents

돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도 Download PDF

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Publication number
WO2010002160A3
WO2010002160A3 PCT/KR2009/003516 KR2009003516W WO2010002160A3 WO 2010002160 A3 WO2010002160 A3 WO 2010002160A3 KR 2009003516 W KR2009003516 W KR 2009003516W WO 2010002160 A3 WO2010002160 A3 WO 2010002160A3
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WO
WIPO (PCT)
Prior art keywords
promoter
present
porcine alpha
casein
target protein
Prior art date
Application number
PCT/KR2009/003516
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English (en)
French (fr)
Other versions
WO2010002160A9 (ko
WO2010002160A2 (ko
Inventor
여명구
강성조
안종덕
Original Assignee
조아제약주식회사
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by 조아제약주식회사 filed Critical 조아제약주식회사
Priority to CA2729625A priority Critical patent/CA2729625C/en
Priority to AU2009266603A priority patent/AU2009266603B2/en
Priority to CN200980126110XA priority patent/CN102124109B/zh
Priority to JP2011516140A priority patent/JP5507555B2/ja
Priority to US12/737,319 priority patent/US9738694B2/en
Priority to EP09773696.1A priority patent/EP2305814B1/en
Publication of WO2010002160A2 publication Critical patent/WO2010002160A2/ko
Publication of WO2010002160A9 publication Critical patent/WO2010002160A9/ko
Publication of WO2010002160A3 publication Critical patent/WO2010002160A3/ko

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4732—Casein
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/20—Animal model comprising regulated expression system
    • A01K2217/206—Animal model comprising tissue-specific expression system, e.g. tissue specific expression of transgene, of Cre recombinase
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
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    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/108—Swine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
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    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/48—Vector systems having a special element relevant for transcription regulating transport or export of RNA, e.g. RRE, PRE, WPRE, CTE

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
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  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Toxicology (AREA)
  • Veterinary Medicine (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)

Abstract

본 발명은 돼지 알파에스 1 카제인 유전자, 돼지 알파에스 1 카제인 유전자 프로모터, 및 상기 프로모터를 이용한 발현 벡터, 상기 발현 벡터를 이용한 목적단백질의 제조방법을 제공한다. 본 발명의 프로모터는 목적 단백질의 유선특이적 발현을 촉진하므로, 본 발명의 프로모터를 이용하여 형질전환된 동물은 유즙 중에 목적단백질을 고농도로 분비하게 되어 유용단백질의 생산에 유용하게 사용될 수 있다는 장점을 갖는다.
PCT/KR2009/003516 2008-06-30 2009-06-29 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도 WO2010002160A2 (ko)

Priority Applications (6)

Application Number Priority Date Filing Date Title
CA2729625A CA2729625C (en) 2008-06-30 2009-06-29 Gene of porcine alpha-s1-casein, a promoter of the same and the use thereof
AU2009266603A AU2009266603B2 (en) 2008-06-30 2009-06-29 Gene of porcine alpha-s1 casein, a promoter of the same and use thereof
CN200980126110XA CN102124109B (zh) 2008-06-30 2009-06-29 猪α-S1-酪蛋白基因，其启动子及其用途
JP2011516140A JP5507555B2 (ja) 2008-06-30 2009-06-29 豚のαＳ１カゼイン遺伝子、そのプロモーター、及びその用途
US12/737,319 US9738694B2 (en) 2008-06-30 2009-06-29 Gene of porcine alpha-s1 casein, a promoter of the same and use thereof
EP09773696.1A EP2305814B1 (en) 2008-06-30 2009-06-29 Gene of porcine alpha-s1 casein, a promoter of the same and use thereof

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR10-2008-0062765 2008-06-30
KR20080062765 2008-06-30

Publications (3)

Publication Number Publication Date
WO2010002160A2 WO2010002160A2 (ko) 2010-01-07
WO2010002160A9 WO2010002160A9 (ko) 2010-05-06
WO2010002160A3 true WO2010002160A3 (ko) 2010-07-01

Family

ID=41466443

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/KR2009/003516 WO2010002160A2 (ko) 2008-06-30 2009-06-29 돼지의 알파에스 1 카제인 유전자, 그 프로모터 및 그의 용도

Country Status (8)

Country Link
US (1) US9738694B2 (ko)
EP (1) EP2305814B1 (ko)
JP (1) JP5507555B2 (ko)
KR (1) KR101033818B1 (ko)
CN (1) CN102124109B (ko)
AU (1) AU2009266603B2 (ko)
CA (1) CA2729625C (ko)
WO (1) WO2010002160A2 (ko)

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WO2010002082A1 (en) * 2008-06-30 2010-01-07 Cho-A Pharm Co., Ltd. A gene of porcine beta-casein, a promoter of the same and the use thereof
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KR101938956B1 (ko) 2016-05-31 2019-01-15 제주대학교 산학협력단 신경세포 특이적으로 발현하는 돼지 Thy1 유전자 프로모터
KR101928659B1 (ko) * 2017-06-19 2018-12-13 서울대학교병원 Dna 변형 유도 단백질 복합체 및 이를 암호화하는 유전자를 포함하는 재조합 벡터
CN110257392A (zh) * 2019-06-28 2019-09-20 西安医学院 转录后水平低氧调控基因、应用及其调控方法
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
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Also Published As

Publication number Publication date
AU2009266603B2 (en) 2013-01-24
US20110239314A1 (en) 2011-09-29
EP2305814A4 (en) 2011-12-28
EP2305814A2 (en) 2011-04-06
CA2729625A1 (en) 2010-01-07
AU2009266603A1 (en) 2010-01-07
CA2729625C (en) 2016-02-02
US9738694B2 (en) 2017-08-22
EP2305814B1 (en) 2015-08-05
JP5507555B2 (ja) 2014-05-28
CN102124109A (zh) 2011-07-13
KR20100003223A (ko) 2010-01-07
JP2011526495A (ja) 2011-10-13
CN102124109B (zh) 2013-10-16
WO2010002160A9 (ko) 2010-05-06
KR101033818B1 (ko) 2011-05-13
WO2010002160A2 (ko) 2010-01-07

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