Classifications

• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/02—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings
  • C07D401/06—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing two hetero rings linked by a carbon chain containing only aliphatic carbon atoms
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/4427—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems
  • A61K31/4439—Non condensed pyridines; Hydrogenated derivatives thereof containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. omeprazole
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
  • A61K31/00—Medicinal preparations containing organic active ingredients
  • A61K31/33—Heterocyclic compounds
  • A61K31/395—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins
  • A61K31/435—Heterocyclic compounds having nitrogen as a ring hetero atom, e.g. guanethidine or rifamycins having six-membered rings with one nitrogen as the only ring hetero atom
  • A61K31/44—Non condensed pyridines; Hydrogenated derivatives thereof
  • A61K31/445—Non condensed piperidines, e.g. piperocaine
  • A61K31/4523—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems
  • A61K31/454—Non condensed piperidines, e.g. piperocaine containing further heterocyclic ring systems containing a five-membered ring with nitrogen as a ring hetero atom, e.g. pimozide, domperidone
• • A—HUMAN NECESSITIES
  • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
  • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
  • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
  • A61P31/10—Antimycotics
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D401/00—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom
  • C07D401/14—Heterocyclic compounds containing two or more hetero rings, having nitrogen atoms as the only ring hetero atoms, at least one ring being a six-membered ring with only one nitrogen atom containing three or more hetero rings
• • C—CHEMISTRY; METALLURGY
  • C07—ORGANIC CHEMISTRY
  • C07D—HETEROCYCLIC COMPOUNDS
  • C07D487/00—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00
  • C07D487/02—Heterocyclic compounds containing nitrogen atoms as the only ring hetero atoms in the condensed system, not provided for by groups C07D451/00 - C07D477/00 in which the condensed system contains two hetero rings
  • C07D487/10—Spiro-condensed systems

Definitions

• the present invention relates to efinaconazole producing and purifying methods adapted to industrial scale that provide high-purity efinaconazole in high yield by simple operations using specific impurities as indices.
• Efinaconazole is a triazole compound having antifungal activity that is represented by formula (I):
• Efinaconazole is known as an active ingredient of topical therapeutics for onychomycosis and sold under different drug names, in Japan as CLENAFIN® (10% solution for topical application to nails), and in the US as JUBLIA® (10% topical solution).
• Efinaconazole is produced by methods in which (2R, 3S)-2-(2,4-difluorophenyl)-3-methyl-2-[(1H-1,2,4-triazol-1-yl)methyl]oxirane (hereinafter sometimes referred to as epoxytriazole) is subjected to ring-opening addition using 4-methylenepiperidine (hereinafter sometimes referred to as 4-MP) (Patent Documents 1, 2, and 3). Also known are methods for purifying the efinaconazole obtained (Patent Documents 4 and 5).
• a method that comprises neutralizing 4-methylenepiperidine hydrochloride with potassium hydroxide, then adding lithium bromide and epoxytriazole in a toluene solution for reaction to obtain crude efinaconazole, then isolating p-toluenesulfonate of the crude efinaconazole, neutralizing the p-toluenesulfonate with potassium carbonate in a dichloromethane solution, and thereafter performing crystallization from an ethanol-water mixed solvent to purify the efinaconazole (Patent Document 6).
• this method cannot be referred to for the above-described purpose and it involves the problem of prolonged reaction time.
• a method comprises adding a strong organic base tetramethyl guanidine, 4-methylenepiperidine hydrobromide, lithium nitrate and epoxytriazole in acetonitrile for reaction to obtain crude efinaconazole, then isolating p-toluenesulfonate of the crude efinaconazole, neutralizing the p-toluenesulfonate with sodium hydroxide in a methanol-water mixed solvent and adding water for crystallization to purify the efinaconazole (Patent Document 7).
• This method however, has the problem of prolonged reaction time. What is more, this method requires a cumbersome work-up operation since the crude efinaconazole is crystallized even before the p-toluenesulfonate of efinaconazole is formed.
• Patent Document No. 1 WO 94/26734
• Patent Document No. 2 WO 2012/029836
• Patent Document No. 3 WO 2016/079728
• Patent Document No. 4 WO 2016/116919
• Patent Document No. 5 WO 2016/181306
• Patent Document No. 6 WO 2016/193917
• Patent Document No. 7 United States Patent Publication No. 2016/0376253
• the present invention has as its object providing efinaconazole producing and purifying methods adapted to industrial scale that can provide high-purity efinaconazole in high yield by simple operations.
• compound (IV), compound (V), compound (VI), compound (VII), compound (VIII), compound (IX), compound (X), compound (XI), compound (XII), and compound (XIII) could occur as impurities in the process of producing efinaconazole, with the additional discovery that by using these specific impurities as indices from the viewpoints of their amounts and their removability, high-purity efinaconazole could be produced in high yield by simple operations.
• ring-opening addition reaction a method comprising reacting epoxytriazole with an acid addition salt of 4-methylenepiperidine to synthesize efinaconazole (this step is hereinafter sometimes referred to as “ring-opening addition reaction”), then forming p-toluenesulfonate of the efinaconazole and isolating it, then neutralizing the p-toluenesulfonate of efinaconazole to obtain efinaconazole, the duration of ring-opening addition reaction could be shortened by using a specific solvent (toluene) in the reaction but that the impurity compound (IV) increased.
• the increased amount of impurity compound (IV) was difficult to be removed by conventional methods but unexpectedly, the amount of compound (IV) was found to decrease upon adjusting the pH of the aqueous layer while washing the reaction mixture after the reaction.
• the present inventors further discovered that in the step of isolating the p-toluenesulfonate of efinaconazole, it is possible to remove compound (XIII) that is so close to efinaconazole in terms of physicochemical behavior that no method has yet been established for its analysis or removal and have led to the establishment of a method for analyzing compound (XIII).
• the present inventors further discovered that in the step of crystallizing efinaconazole using an ethanol-water mixed solvent, the crystals of efinaconazole was obtained in high yield by adding water after the crystals of efinaconazole has formed in the solution.
• the present invention provides the following.
• a method of producing efinaconazole comprising:
• step A of forming a toluene solution comprising a compound represented by formula (II):
• epoxytriazole (hereinafter sometimes referred to as epoxytriazole);
• step B of subjecting the toluene solution to reaction under heating; step C of washing the reaction mixture from step B, specifically washing the same more than once or washing so that the pH of the aqueous layer after the washing operation is between 3 and 5, thereby obtaining a toluene solution of crude efinaconazole in which the residual amount of 4-MP is not more than 5 wt % of efinaconazole; step D of mixing the toluene solution of crude efinaconazole with 2-propanol and p-toluenesulfonate or a hydrate thereof to precipitate p-toluenesulfonate of efinaconazole; step E of isolating the p
• the compound is hereinafter sometimes referred to as 4-MP.HBr salt
• 4-MP.HBr salt which is contained in an amount of 1-1.6 moles relative to one mole of epoxytriazole and wherein the inorganic base is lithium hydroxide or a hydrate thereof and contained in an amount of 1-1.6 moles relative to one mole of epoxytriazole.
• step F in the efinaconazole as obtained after step F is no more than 0.50% (HPLC area percentage).
• step G of forming a solution of the obtained efinaconazole in an ethanol-water mixed solvent and crystallizing the efinaconazole; step H of further adding water and isolating the efinaconazole that has precipitated out; wherein the content of the compound represented by formula (IV) in the efinaconazole as obtained after step H is no more than 0.10% (HPLC area percentage).
• step G The method as recited in [5], wherein the solution in ethanol-water mixed solvent in step G is one that uses a 50-65% ethanol-water mixed solvent.
• step H The method as recited in either [5] or [6], wherein the amount of water to be added in step H is such that the ethanol concentration in the solution is 35-45%.
• step H the compound (the compound is hereinafter sometimes referred to as compound (V)) in the efinaconazole as obtained after step H is no more than 0.50% (HPLC area percentage).
• step H the compound (the compound is hereinafter sometimes referred to as compound (VI)) in the efinaconazole as obtained after step H is no more than 0.15% (HPLC area percentage).
• step H in the efinaconazole as obtained after step H is no more than 0.15% (HPLC area percentage).
• step H in the efinaconazole as obtained after step H is no more than 0.15% (HPLC area percentage).
• step H the compound (the compound is hereinafter sometimes referred to as compound (IX)) in the efinaconazole as obtained after step H is no more than 0.10% (HPLC area percentage).
• step H the compound (the compound is hereinafter sometimes referred to as compound (X)) in the efinaconazole as obtained after step H is no more than 0.10% (HPLC area percentage).
• step H in the efinaconazole as obtained after step H is no more than 0.10% (HPLC area percentage).
• step H the compound (the compound is hereinafter sometimes referred to as compound (XIII)) in the efinaconazole as obtained after step H is no more than 0.10% (HPLC area percentage).
• Efinaconazole wherein the content of compound (IV) is 0.10% or less, the content of compound (V) is 0.50% or less, the content of compound (VI) is 0.15% or less, the content of compound (VII) is 0.15% or less, the content of compound (VIII) is 0.15% or less, the content of compound (IX) is 0.10% or less, the content of compound (X) is 0.10% or less, the content of compound (XI) is 0.10% or less, the content of compound (XII) is 0.10% or less, and the content of compound (XIII) is 0.10% or less, as well as the purity of efinaconazole is no less than 98.0% and each value of percentage is HPLC area percentage.
• Efinaconazole wherein the content of compound (IV) is 0.10% or less, the content of compound (V) is 0.15% or less, the content of compound (VI) is 0.15% or less, the content of compound (VII) is 0.15% or less, the content of compound (VIII) is 0.15% or less, the content of compound (IX) is 0.10% or less, the content of compound (X) is 0.10% or less, the content of compound (XI) is 0.10% or less, the content of compound (XII) is 0.10% or less, and the content of compound (XIII) is 0.10% or less, as well as the purity of efinaconazole is no less than 98.0% and each value of percentage is HPLC area percentage.
• Efinaconazole wherein the content of compound (IV) is 0.05% or less, the content of compound (V) is 0.50% or less, the content of compound (VI) is 0.05% or less, the content of compound (VII) is 0.05% or less, the content of compound (VIII) is 0.05% or less, the content of compound (IX) is 0.05% or less, the content of compound (X) is 0.05% or less, the content of compound (XI) is 0.05% or less, the content of compound (XII) is 0.05% or less, and the content of compound (XIII) is 0.05% or less, as well as the purity of efinaconazole is no less than 99.0% and each value of percentage is HPLC area percentage.
• Efinaconazole wherein the content of compound (IV) is 0.05% or less, the content of compound (V) is 0.15% or less, the content of compound (VI) is 0.05% or less, the content of compound (VII) is 0.05% or less, the content of compound (VIII) is 0.05% or less, the content of compound (IX) is 0.05% or less, the content of compound (X) is 0.05% or less, the content of compound (XI) is 0.05% or less, the content of compound (XII) is 0.05% or less, and the content of compound (XIII) is 0.05% or less, as well as the purity of efinaconazole is no less than 99.0% and each value of percentage is HPLC area percentage.
• a method for measuring the purity of efinaconazole which is characterized by analyzing compound (IV), compound (V), compound (VI), compound (VII), compound (VIII), compound (X), compound (XI) and compound (XII) as indices for impurities by HPLC.
• high-purity efinaconazole can be provided in high yield by simple operations adapted to industrial scale with reference made to specific impurities as indices.
• toluene solution in step A refers to a solution using toluene as the major solvent and it may be a mixed solution containing other solvents. Preferably, it is a solution containing no less than 90% of toluene, more preferably, a solution containing no less than 99% of toluene.
• the amount of toluene to be used in step A is 2-5 times the mass (kg) of epoxytriazole. Due to this small amount of toluene, the reaction time in step B can be shortened.
• epoxytriazole, compound (III) and an inorganic base are added to toluene is not particularly limited and they may be added simultaneously.
• Symbol X in compound (III) represents Cl, Br or I, preferably Br.
• the equivalent amount of compound (III) is preferably 1.1-1.6 equivalents, more preferably 1.5 equivalents, per equivalent of epoxytriazole.
• the “inorganic base” in step A is preferably lithium hydroxide or a hydrate thereof.
• the equivalent amount of the inorganic base is preferably 1.1-1.6 equivalents, more preferably 1.5 equivalents, per equivalent of epoxytriazole.
• the reaction temperature in step B is not particularly limited as long as the reaction proceeds fast enough and the contents of impurities are not increased. It is preferably 60-110° C., more preferably 70-90° C.
• the reaction time in step B is not particularly limited as long as the reaction proceeds and the contents of impurities are not increased. It is preferably 1-22 hours, more preferably 1-15 hours, even more preferably 1-12 hours.
• Washing in step C is not particularly limited as long as the residual unreacted 4-MP can be removed and the recovery of the produced efinaconazole is high. Washing may be done either once or, if necessary, more than once.
• the residual amount of 4-MP greatly affects the yield of efinaconazole in step D and subsequent purification steps, so at the time when the work-up operation of step C ends, the residual amount of 4-MP is preferably 5 wt % or less, more preferably 2 wt % or less, of the efinaconazole in the toluene solution of crude efinaconazole.
• “Washing” in step C is more preferably performed with “an acidic aqueous solution.”
• washing can be done using an acidic aqueous solution such as an aqueous solution of ammonium chloride, hydrochloric acid, an aqueous solution of phosphoric acid, or an aqueous solution of acetic acid.
• an acidic aqueous solution such as an aqueous solution of ammonium chloride, hydrochloric acid, an aqueous solution of phosphoric acid, or an aqueous solution of acetic acid.
• washing with hydrochloric acid for example, may be followed by washing with an aqueous solution of acetic acid.
• washing with water may be followed by washing with an aqueous solution of ammonium chloride, then with an aqueous solution of acetic acid.
• the pH of the aqueous layer after the washing operation is preferably 3-8, more preferably 3-5, even more preferably 3.5-4.5.
• an impurity represented by formula (IV) for example, but this can be removed into the aqueous layer by making pH adjustment to one of the ranges noted above.
• the content of the impurity represented by formula (IV) is reduced to 0.5% and less at the time when step F ends.
• the solvent may, depending on the need, be distilled off for concentration, whereupon “the toluene solution of crude efinaconazole” is obtained.
• the amount of “p-toluenesulfonate or a hydrate thereof” in step D is preferably 1.0-1.2 equivalents, more preferably 1.1 equivalents, per equivalent of the crude efinaconazole.
• step D the reaction mixture is heated and then cooled, whereupon a p-toluenesulfonate of efinaconazole is precipitated.
• a mixed solvent containing water, methanol, or ethanol, etc. may be employed.
• step E of isolating the p-toluenesulfonate of efinaconazole enables removal of compound (XIII) which is so close to efinaconazole in terms of physicochemical behavior that no method for its analysis or removal has yet been established.
• the neutralizing agent to be used in step F is not particularly limited and may preferably be exemplified by NaHCO 3 .
• the solvent to be used is not particularly limited and may preferably be exemplified by an ethyl acetate-water mixed liquid.
• the ethanol-water mixed solvent to be used in step G is not particularly limited as long as efinaconazole crystallizes and the ethanol concentration is preferably 50-70%, more preferably 50-65%, even more preferably 58-62%, and most preferably 60%.
• the amount of water to be added in step H is determined from the viewpoints of the yield of the objective product efinaconazole and the contents of impurities and it is preferably such that the ethanol concentration in the reaction mixture after the addition of water is 35-45%, more preferably 40%.
• the time of ring-opening addition reaction in step B can be shortened by using toluene in a volume (L) which is 2-5 times the mass (kg) of epoxytriazole; on the other hand, this usually increases the amount of compound (IV).
• the amount of compound (IV) can be reduced by the washing operation in step C of the present invention. Briefly, the content of compound (IV) decreases upon adjusting the pH of the aqueous layer in the process of washing the reaction mixture from the ring-opening addition reaction.
• Compound (XIII) is so close to efinaconazole in physicochemical behavior that no method for its analysis or removal has yet been established.
• the present invention enables the removal of compound (XIII), thereby enabling the production of high-purity efinaconazole.
• Compound (XIII) is mainly removed in step E.
• the method for measuring the purity of efinaconazole in accordance with the present invention is characterized by analyzing compound (IX) as an index of impurity.
• the method uses a column packed with cellulose derivatives bonded silica gel in HPLC analysis.
• the mobile phase is preferably a mixed solvent comprising of a potassium hexafluorophosphate buffer and acetonitrile which are preferably mixed at a ratio in the range of 90-10:10-90, more preferably in the range of 70-60:30-40, even more preferably in the range of 64-61:36-39.
• the gradient method may be adopted and in that case, a mixed solvent with a mixing ratio in one of the ranges set forth above may be used in at least part of the method.
• the pH of the potassium hexafluorophosphate buffer is preferably 7 or less, more preferably in the range of 1.8-3.0, and even more preferably in the range of 1.9-2.1.
• the salt concentration of the potassium hexafluorophosphate buffer is preferably between 0.05 mol/L and 0.5 mol/L, more preferably 0.1-0.4 mol/L, and even more preferably 0.25-0.35 mol/L.
• compound (IX) and efinaconazole are separated appropriately so that the purity of efinaconazole and the content of compound (IX) can be measured.
• the method for measuring the purity of efinaconazole is characterized by analyzing compound (XIII) as an index of impurity.
• the method uses a column packed with octadecylsilanized silica gel in HPLC analysis.
• the mobile phase is preferably a mixed solvent comprising of an ammonium hydrogencarbonate aqueous solution and acetonitrile which are preferably mixed at a ratio in the range of 90-10:10-90, more preferably in the range of 70-15:30-85, even more preferably in the range of 60-45:40-55, and particularly preferably in the range of 55-51:45-49.
• the gradient method may be adopted and in that case, a mixed solvent with a mixing ratio in one of the ranges set forth above may be used in at least part of the method.
• the salt concentration of the ammonium hydrogencarbonate aqueous solution is preferably between 0.001 mol/L and 0.05 mol/L, more preferably 0.005-0.015 mol/L.
• compound (XIII) and efinaconazole are separated appropriately so that the purity of efinaconazole and the content of compound (XIII) can be measured.
• the method for measuring the purity of efinaconazole is characterized by analyzing compound (IV), compound (V), compound (VI), compound (VII), compound (VIII), compound (X), compound (XI) and compound (XII) as indices of impurities.
• the method uses a column packed with octadecylsilanized silica gel in HPLC analysis.
• the mobile phase is preferably a water/acetonitrile mixed solvent to which is added trifluoroacetic acid, with the mixing ratio being preferably in the range of 90-10:10-90, more preferably in the range of 90-50:10-50, even more preferably in the range of 85-50:15-50.
• the trifluoroacetic acid added preferably accounts for 0.01% to 0.5% of the mobile phase.
• the gradient method may be adopted and in that case, a mixed solvent with a mixing ratio in one of the ranges set forth above may be used in at least part of the method.
• compound (IV), compound (V), compound (VI), compound (VII), compound (VIII), compound (X), compound (XI) and compound (XII) are appropriately separated from efinaconazole so that the purity of efinaconazole and the contents of those impurity compounds can be measured.
• Epoxytriazole (20.00 g, 0.080 mol), 4-MP.HBr salt (21.26 g, 0.119 mol), lithium hydroxide (2.86 g, 0.119 mol) and acetonitrile (80 mL) were mixed and heated under reflux for 16 hours. After cooling the reaction mixture, ethanol (80 mL) and water (120 mL) were added. After cooling to 5° C. and below, seed crystals of efmaconazole were added and the mixture was stirred for crystallization. After adding water (360 mL) and stirring the mixture at room temperature, the resulting crystals were filtered and dried to obtain crude efinaconazole (yield: 22.49 g, 81%).
• Example 1a (Using Toluene as a Reaction Solvent in Steps a to C and Evaluating the Reaction Time in Step B)
• Toluene solutions of crude efinaconazole containing different contents of 4-MP were prepared and each of these toluene solutions was mixed with 2-propanol and p-toluenesulfonate (PTSA) monohydrate (1.1 eq.), followed by precipitating a p-toluenesulfonate of efinaconazole, which was isolated to calculate the yield of efinaconazole.
• PTSA 2-propanol and p-toluenesulfonate
• Detector Hydrogen flame ionization detector
• Column A fused silica tube 0.32 mm in internal diameter and 30 m in length, coated with 5% phenyl/95% dimethylpolysiloxane for gas chromatography of 1.00 ⁇ m thickness.
• Column temperature Maintain at 80° C. for 10 min, then increase gradually to 250° C. at the rate of 25° C. per minute and maintain at 250° C. for 5 minutes.
• Injection temperature A constant temperature of about 250° C.
• Detector temperature A constant temperature of about 290° C.
• Carrier gas Helium
• the amount of 4-MP is preferably 5 wt % or less, more preferably 2 wt % or less, of efinaconazole.
• the reaction mixture prepared in Example 1A (reaction time: 10 hours) was cooled and mixed with hydrochloric acid for adjusting the pH of the aqueous layer to 6.8; thereafter, the aqueous layer 1 was removed to obtain an organic layer 1.
• the organic layer 1 was washed with an aqueous solution of acetic acid or phosphoric acid at different concentrations so that the pH of the aqueous layer would vary, whereupon an organic layer 2 and an aqueous layer 2 were obtained.
• the organic layer 2 and the aqueous layer 2 were analyzed by high-performance liquid chromatography to calculate the HPLC area percentage of compound (IV) and the amount of efinaconazole loss into the aqueous layer 2.
• Epoxytriazole (1.0 kg, 3.98 mol), 4-MP.HBr salt (1.06 kg, 5.95 mol), lithium hydroxide (143 g, 5.97 mol) and toluene (2 L) were mixed and stirred at 95-105° C. for 5.8 hours.
• the reaction mixture was cooled and mixed with water.
• the aqueous layer was removed and the organic layer was successively washed with an aqueous solution of ammonium chloride and an aqueous sodium chloride solution to obtain a toluene solution of crude efinaconazole.
• Epoxytriazole (100 g, 0.398 mol), 4-MP.HBr salt (106.4 g, 0.598 mol), lithium hydroxide (14.3 g, 0.597 mol) and toluene (400 mL) were mixed and stirred at 80° C. for 11.5 hours.
• the reaction mixture was cooled and mixed with hydrochloric acid to adjust the pH of the aqueous layer to 6.0.
• the aqueous layer was removed and the organic layer was successively washed with an aqueous solution of acetic acid (the pH of the aqueous layer after the washing was 4.3) and water (the pH of the aqueous layer after the washing was 4.6).
• the resulting solution was concentrated to obtain a toluene solution of crude efinaconazole (238.87 g, net content of efinaconazole: 124.6 g, yield: 90%).
• a toluene solution of crude efinaconazole (95.83 g, net content of efinaconazole: 50.0 g, 0.144 mol),p-toluenesulfonate monohydrate (30.03 g, 0.158 mol), 2-propanol (600 mL) and water (2.5 g) were mixed and heated to form a solution.
• the solution was cooled to 5° C. or below for crystallization.
• the p-toluenesulfonate of efinaconazole as wet crystals (80.0 g, net content of p-toluenesulfonate of efinaconazole: 63.36 g, 0.122 mol), ethyl acetate (441 mL), water (441 mL) and sodium hydrogencarbonate (11.16 g, 0.133 mol) were mixed and neutralized. After removing the aqueous layer by liquid-liquid separation, the organic layer was washed with water to obtain an organic layer. The organic layer was concentrated under reduced pressure and to the residue, ethanol (205 mL) and water (137 mL) were added, followed by heating to form a solution.
• Test Example 1 Analysis Method: To Analyze Compound (IV), Compound (V), Compound (VI), Compound (VII), Compound (VIII), Compound (X), Compound (XI), and Compound (XII))
• Efinaconazole as obtained in the Reference Example and in Examples 2 to 4 was dissolved in methanol to prepare 1 mg/mL solutions, which were used as sample solutions. A 15 ⁇ L portion of each sample solution was tested by liquid chromatography under the following conditions and the respective peak areas were measured by automatic integration.
• Detector UV absorption photometer (measurement wavelength: 262 nm)
• Column A stainless steel column 4.6 mm in inside diameter and 15 cm in length, packed with 5 ⁇ m in particle diameter of octadecylsilanized silica gel for liquid chromatography. Column temperature: A constant temperature of about 40° C.
• Mobile phase A A mixture of water/trifluoroacetic acid (1000:1)
• Mobile phase B A mixture of acetonitrile/trifluoroacetic acid (1000:1)
• Flow of mobile phases Gradient was controlled by mixing the mobile phases A and B as directed in Table 4.
• the content of compound (XIII) was determined by a method based on high-performance liquid chromatography (HPLC).
• Efinaconazole as obtained in the Reference Example and in Examples 2 to 4 was dissolved in a dissolving solution to prepare 10 mg/mL solutions, which were used as sample solutions. A 15 ⁇ L portion of each sample solution was tested by liquid chromatography under the following conditions and the respective peak areas were measured by automatic integration.
• Dissolving solution A mixture of 0.05 mol/L ammonium hydrogencarbonate aqueous solution/acetonitrile (53:47)
• Detector UV absorption photometer (measurement wavelength: 262 nm)
• Column A stainless steel column 4.6 mm in inside diameter and 15 cm in length, packed with 3 ⁇ m in particle diameter of octadecylsilanized silica gel for liquid chromatography.
• Column temperature A constant temperature of about 40° C.
• Mobile phase A 0.01 mol/L ammonium hydrogencarbonate aqueous solution
• Mobile phase B Acetonitrile
• Gradient was controlled by mixing the mobile phases A and B as directed in Table 5.
• the content of compound (IX) was determined by a method based on high-performance liquid chromatography (HPLC).
• Efinaconazole as obtained in the Reference Example and in Examples 2 to 4 was dissolved in a dissolving solution to prepare 15 mg/mL solutions, which were used as sample solutions. A 10 ⁇ L portion of each sample solution was tested by liquid chromatography under the following conditions and the respective peak areas were measured by automatic integration.
• Dissolving solution A mixture of 0.3 mol/L potassium hexafluorophosphate buffer (pH 2.0)/acetonitrile (1:1)
• Detector UV absorption photometer (measurement wavelength: 262 nm)
• Column A stainless steel column 4.6 mm in inside diameter and 15 cm in length, packed with 5 ⁇ m in particle diameter of cellulose derivatives bonded silica gel for liquid chromatography (silica gel coated with cellulose derivatives)
• Column temperature A constant temperature of about 25° C.
• Mobile phase A mixture of 0.3 mol/L potassium hexafluorophosphate buffer (pH 2.0)/acetonitrile (63:37)
• Flow rate About 0.5 mL per minute Time span of measurement: 30 minutes (excluding the solvent peak)
• the method of the present invention for producing efinaconazole can provide high-purity efinaconazole in high yield by simple operations.
• high-purity efinaconazole can be provided in high yield by simple operations using specific impurities as indices, with the result that there can be provided methods for producing and purifying efinaconazole that are adapted to industrial scale.

Landscapes

• Chemical & Material Sciences (AREA)
• Organic Chemistry (AREA)
• Health & Medical Sciences (AREA)
• Life Sciences & Earth Sciences (AREA)
• Veterinary Medicine (AREA)
• Public Health (AREA)
• General Health & Medical Sciences (AREA)
• Medicinal Chemistry (AREA)
• Animal Behavior & Ethology (AREA)
• Pharmacology & Pharmacy (AREA)
• General Chemical & Material Sciences (AREA)
• Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
• Chemical Kinetics & Catalysis (AREA)
• Oncology (AREA)
• Communicable Diseases (AREA)
• Epidemiology (AREA)
• Plural Heterocyclic Compounds (AREA)
• Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
• Nitrogen Condensed Heterocyclic Rings (AREA)
• Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
• Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)

Priority Applications (1)

Applications Claiming Priority (3)

Related Parent Applications (1)

Related Child Applications (1)

Publications (1)

Family

ID=64274211

Family Applications (2)

Family Applications After (1)

Country Status (11)

Citations (7)

Family Cites Families (12)

• 2018
  • 2018-05-18 JP JP2018563536A patent/JP7203612B2/ja active Active
  • 2018-05-18 CA CA3063341A patent/CA3063341A1/en active Pending
  • 2018-05-18 KR KR1020197035809A patent/KR102597119B1/ko active IP Right Grant
  • 2018-05-18 PL PL18802671.0T patent/PL3628668T3/pl unknown
  • 2018-05-18 EP EP18802671.0A patent/EP3628668B1/en active Active
  • 2018-05-18 CN CN201880030101.XA patent/CN110770220B/zh active Active
  • 2018-05-18 ES ES18802671T patent/ES2941251T3/es active Active
  • 2018-05-18 TW TW107117086A patent/TWI772425B/zh active
  • 2018-05-18 WO PCT/JP2018/019324 patent/WO2018212333A1/ja active Application Filing
  • 2018-05-18 IL IL270691A patent/IL270691B/en unknown
• 2019
  • 2019-06-11 US US16/438,011 patent/US20190292166A1/en not_active Abandoned
• 2020
  • 2020-02-19 US US16/795,275 patent/US10829475B2/en active Active

Patent Citations (7)

Also Published As

Similar Documents

Legal Events