KR20110109568A - The whitening cosmetic composition using beer yeast - Google Patents

Abstract

The present invention relates to a whitening cosmetic composition containing a grain fermented extract fermented with brewer's yeast. More specifically, malt, rice bran and wheat as the main ingredients in the fermentation stage of the beer manufacturing process, gelatinized and saccharified, aged, and then filtered and concentrated by low-temperature ultrasonic extraction, fermented with brewer's yeast It is to provide a whitening cosmetic composition using brewer's yeast containing fermented extract.

Description

Whitening cosmetic composition using beer yeast {The whitening cosmetic composition using beer yeast}

The present invention relates to a whitening cosmetic composition. Specifically, it relates to a whitening cosmetic composition using beer yeast containing grain fermented extract fermented with beer yeast.

Generally, the skin's epidermis is darkened when the skin is exposed to ultraviolet light. This is due to the synthesis and release of melanin in the melanocytes of the skin, melanin is produced by a spontaneous oxidation reaction from the dopaquinone by the action of an enzyme called tyrosinase with the tyrosine in the cell substrate .

Therefore, in order to prevent the skin from becoming black, it is the simplest and most common way to reduce the production of melanin by inhibiting some reactions in the melanogenesis reaction. To this end, conventionally, hydroquinone (JP-A-192062), ascorbic acid (JP-A 4-9320), kojic acid (JP-A-56-7710), arbutin (JP-A 4-93150), glutathione and lettuce extract It is known to use a variety of extracts, including melamine production inhibitors.

Of these, ascorbic acid, baekryepi extract, glutathione, etc. have been used to prevent skin blackening or skin disease, but they have been used to limit the skin irritation and low stability when formulated as a cosmetic. In addition, hydroquinone is already used as a drug for the purpose of treating blemishes, but it has been prescribed as a skin irritant and carcinogenic substance and has been recently banned. Ascorbic acid has a problem of stability to light and heat, and various approaches have been taken to secure stability, but the cost to pay for this is difficult to apply the product.

Korean Patent Registration No. 10-0616455 discloses a cosmetic composition using saccharomyces sp yeast extract as a skin whitening ingredient.

Saccharomyces yeast extract is acidic (pH 5 ~ 6) and has a yeast-specific odor and a dark yellow color. Therefore, it is difficult to use it as a cosmetic ingredient. The reason why the direct extract of yeast is colored is that in the production process of yeast extract, sugar and amino acids undergo an aminocarbonyl reaction by heat treatment to produce a substance having a dark brown color. In view of this background, Korean Patent Registration No. 10-0522945 discloses the purification extract of yeast extract through heat extraction process, filtration and adsorption process to remove coloring components and odor-specific odor components from Saccharomyces yeast extract. And it discloses a method of using this as a component of the cosmetic.

In order to use the direct extract of Saccharomyces yeast as a component of cosmetics, complicated post-treatment process is required, and even after complex post-treatment process, complete purification is difficult, so it is quite limited to use as a component of cosmetics. there is a problem.

It is an object of the present invention to provide a colorless, odorless skin irritation-free whitening cosmetic composition components and natural whitening cosmetic composition with high stability of the formulation when formulated in cosmetics and excellent whitening performance.

The present inventors have completed the present invention by confirming that the fermented grain extract fermented with brewer's yeast shows an excellent whitening effect and is almost colorless and has no off-flavor, rich skin nutritional ingredients, and excellent formulation stability when formulated in cosmetics.

The present invention relates to a whitening cosmetic component of a grain fermented extract fermented with brewer's yeast and a whitening cosmetic composition containing the same.

Grain used in the present invention is a mixture of malt, wheat, rice bran is suitable.

Malt refers to germinated barley, which is mainly used to produce beverages such as beer because it is decomposed into starch and protein by protease such as α-amylase and β-amylase to obtain soluble substances such as reducing sugars and free amino acids. It is used.

Wheat ( Triticum aestivum L) belongs to the family of rice, and contains sugars, fats, proteins, fiber, vitamins, and the like. Especially, lipopolysaccharides contained in wheat are known to enhance immunity.

Rice bran (Rice bran) is a mixture of skin, seedling, whistle layer, etc., which is produced when milling brown rice and making white rice. It is also called rice bran. Rice bran is rich in protein, fat, sugars, vitamin B and vitamin E (AACC Inc, 647-687 (1985)), and antioxidants of rice bran (J. Oleo Sci., 55 (11), 585-591 ( 2006), 40), anti-cancer and anti-allergic (J. Korean Soc. Food Sci. Nutr., 35 (10), 1297-1303 (2006), 41). Attempts have been made to explore the physiological activity of fermented rice bran. Anti-stress and anti-fatigue effects were obtained when fermented rice bran extract fermented with Saccharomyces cerevisae was administered to rats (Phytother. Res., 169, 700-702 (2002)). , 46), Lentinus The edodes fermented rice bran polysaccharide describes the anticancer effect (J. Med. Food, 10 (1), 25-31 (2007), 47).

In the present invention, as a grain for preparing the fermented extract fermented with brewer's yeast, a mixture of malt, rice bran and wheat is suitable, and malt 40 to 70% by weight, 15 to 30 parts by weight of rice bran, wheat 15 to 30 by weight It is suitable to configure it with negative.

The mixture of malt, rice bran, and wheat is subjected to wet heat treatment at a temperature of 80 to 100 ° C. for 30 minutes to 1 hour to lower harmful microbial content and to activate nutrients, and then fermented by adding 0.1 to 10 parts by weight of brewer's yeast. After the step, and aged through the filtration step, and concentrated to add purified water, the extract is recovered at a low temperature of 30 ~ 60 ℃ temperature by low temperature ultrasonic extraction method and concentrated to primary purification. To further purify, the low molecular weight material may be removed by dialysis using a membrane filter having a molecular weight of 10 kD at 4 ° C. Distilled water in the same volume as the separated solution was added to lower the viscosity of the extract, followed by spray drying to determine dry weight to determine productivity.

Grain fermented extract fermented with the brewer's yeast thus prepared is added during cosmetic preparation.

The production of grain fermented extract fermented with brewer's yeast of the present invention uses a low-temperature ultrasonic extraction method that improves the extraction efficiency of extraction by dipping as an extraction method.

Here, low temperature means 60 degrees C or less. Thus, chemical effects on the change of the composition of the fermented grain fermented extract fermented with brewer's yeast, which may occur during extraction, were minimized. The ultrasonic extraction method stabilizes the unstable structure of various useful components and has an advantage of effectively extracting the active ingredient of natural products by penetrating deeply into plant raw materials by various ranges of ultrasonic waves.

In the ultrasonic extraction method, the ultrasonic wave refers to a high frequency sound that is inaudible to the human ear and has a frequency of 40 to 90 kHz.

Ultrasonic extraction method used in the production of grain fermented extract fermented with brewer's yeast of the present invention has the advantage that the strength of the ultrasonic energy change can be adjusted. Thus, the extraction efficiency is very high and the possibility of denaturation and destruction of the active ingredient exhibiting pharmacological activity is minimized, and the production speed is faster than that of the conventional hydrothermal extraction method, which shortens the extraction time and thus has the characteristics of low cost and low energy use.

It also has the advantage that it is possible to remove off-flavor-specific odor.

To the grain fermented extract fermented with brewer's yeast is added an organic solvent or purified water containing 1 to 5 times ethanol or butylene glycol is added during cosmetic preparation.

When the fermented extract fermented with brewer's yeast according to the present invention is used in the cosmetic composition, the fermented extract fermented with the brewer's yeast consisting of malt, rice bran and wheat prepared in the above-described method is 0.01 to 10% by weight, preferably 0.01 to 5% by weight. It is characterized by containing as%. At this time, when the addition of 0.01% by weight or less, the whitening effect of the present invention is inadequate, and when used more than 10% by weight, since the smell and color peculiar to the fermented product start to appear in the final product, it is difficult to apply the product to 5% by weight. The following is suitable.

The cosmetic composition containing the fermented cereal extract fermented with brewer's yeast excellent in whitening effect according to the present invention may have a conventional formulation, for example, supple cosmetics, astringent cosmetics, nutrient cosmetics, nutrition cream, body lotion, body oil , Body essences, cleansers, packs and the like. The composition of each of these formulations may contain various bases and additives necessary and appropriate for the formulation of the formulation, and the types and amounts of these components can be easily selected by those skilled in the art.

The whitening cosmetic composition of the present invention is almost colorless, odorless and has the effect of excellent whitening performance without skin irritation.

Hereinafter, the present invention will be described in detail through examples.

Example 1 Preparation of Grain Fermented Extract Fermented with Beer Yeast

Dry malt, rice bran, and wheat were used as raw materials for the preparation of the fermented cereal grains fermented with brewer's yeast. A grain mixture consisting of 50 g of malt, 25 g of rice bran and 25 g of wheat is immersed in 200 g of hot water at 90 ° C. and aged for 30 minutes to 1 hour to be saccharified through the gelatinization process as in the conventional grain production process. . After saccharification, wort and saccharified residue were filtered off. After the precipitated wort is cooled to room temperature (18 ~ 25 ℃), it is brewer's yeast ( Saccharomyces) cerevisiae ) 1ml (0.3% by weight) is added and aged in the fermentation tank for 7-14 days to obtain 50g of fermentation broth.

Here, the brewer's yeast strain was 5 ~ 7 × 10 ⁷ / ㎖ yeast strain was used.

The fermentation broth was concentrated to dryness by spray concentration to obtain 2 g of the fermentation broth extract powder.

When this is added to a mixed solution composed of 15 ml of purified water and 35 ml of 1.3-butylene glycol, a suspension in which the fermentation broth extract powder is dispersed in the mixed solution is obtained. When the suspension is irradiated with ultrasonic waves at a low temperature of 50 ° C., the useful components in the fermentation broth extract are extracted and transferred to the mixed solution, and the insoluble components remain as residues.

Filtration of this resulted in separation of the residue and fermentation of fermented brewer's yeast with filtrate to yield 50 g. Suspending the fermentation broth extract powder in purified water and 1.3-butylene glycol mixed solution is not suitable for use as a cosmetic composition in the form of fermentation broth powder is very suitable for maintaining the stability of the formulation when formulated as a cosmetic composition Because.

The liquid grain fermented extract fermented with brewer's yeast obtained in this example is a odorless and pale yellow transparent liquid. When formulated as a cosmetic composition, it is almost colorless and does not follow restrictions due to color.

Brewer's yeast used in this embodiment is Saccharomyces genus yeast, such as Saccharomyces cerevisiae, Saccharomyces carlsbergenesis or Saccharomyces bayanus Any of these can be selected.

Example 2 Preparation of Skin Lotion According to the Invention

Skin lotion was prepared at the composition ratio as shown in Table 2 below. The alcohol part was prepared by dissolving Methyl paraben, Polysorbate 20, and Nonoxynol-12 in ethanol in the order, and the purified water part was dissolved in sterilized purified water of Glycerine, 1,3-Butylene glycol, Polyethyleneglycol-12 dimethicone, Citric acid, Sodium citrate. Prepared by dissolving in order and then mixed with the fermented grains fermented with brewer's yeast prepared in Example 1 to prepare a skin lotion.

In Table 2, the composition consisting of an alcohol portion and purified water portion shows the composition of a conventional skin lotion.

Classification Raw material name Weight (% by weight) Alcohol part Ethanol 7 Methyl paraben 0.1 Polysorbate-20 0.2 Nonoxynol-12 0.2 Purified water Purified water 90.51 Glycerin 0.65 1,3-Butylene glycol 0.2 Polyethylene-12 dimethicone 0.1 Citric acid 0.01 Sodium citrate 0.03 Whitening agent Grain fermented extract fermented with brewer's yeast 1.0 Sum 100

Example 3 Preparation of Milk Lotion According to the Invention

As shown in Table 3, the alcohol part was prepared by dissolving in order of Stearic acid, Cetyl alcohol, Mineral oil, Squalene, Isopropyl Myristate, Glyceryl Stearate, Sorbitan Stearate, Polysorbate-20, Isostearyl isostearate, Propyl paraben, Dimethicone in ethanol, The purified water portion was prepared by dissolving Glycerine, Triethanolamine, Propyleneglycol, and Methyl paraben in the sterilized purified water in order, and then mixing the fermented grains fermented with beer yeast prepared in Example 1 to prepare a milk lotion.

In Table 3, the composition consisting of an alcohol part and a purified water part shows the composition of a conventional skin lotion.

Classification Raw material name Weight (% by weight) Alcohol part Stearic acid 2.1 Cetyl alcohol 1.3 Mineral oil 7.0 Squalane 0.5 Isopropyl Myristate 1.0 Glyceryl Stearate 0.5 Sorbitan Stearate 0.85 Polysorbate-20 0.1 Isostearyl isostearate 0.5 Propyl paraben 0.1 Dimethicone 0.1 Purified water Purified water 74.15 Triethanolamine 0.7 Propyleneglycol 7.0 Glycerin 3.0 Methyl paraben 0.1 Whitening agent Grain fermented extract fermented with brewer's yeast 1.0 Sum 100

Experimental Example 1: Skin irritation test of the skin lotion according to the present invention

Apply to the forearm of 40 healthy men and women (20 men, 20 women) by applying a predetermined amount (10m / 5cm2) of the skin lotion prepared in Example 2, and shielding the other parts with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, the skin lotion was not phototoxic because no side effects such as erythema occurred on the skin.

Experimental Example 2: Skin irritation test of milk lotion according to the present invention

Apply a lotion of milk lotion prepared in Example 3 (10m / 5cm2) to the forearm of 40 healthy men and women (20 males, 20 females) and shield the rest with aluminum foil for 30 minutes. Subsequently, long wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, no side effects such as erythema occurred on the skin, and the milk lotion was confirmed to have no phototoxicity.

Experimental Example 3: Skin stability test of skin lotion and milk lotion according to the present invention

The skin patch and milk lotion prepared in Examples 3 and 4 were subjected to a human patch test on the forearm of 40 healthy adult men and women (20 males and 20 females). Wipe the forearm of the subject well with 70% ethanol, and then place a pin chamber containing 20 μl of the skin lotion and milk lotion prepared in Examples 2 and 3 on a conventional cosmetic base using adhesive tape for 24 hours. Closed attachment. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. Experimental results As shown in Table 4, the human patch test to confirm the skin irritation of the skin lotion and milk lotion according to the present invention did not show a positive response was confirmed that there is no irritation to the human skin.

sample 24 hours later 48 hours later Remarks Skin lotion 0/40 0/40 Number of reaction quantums / total number of targets Milk Croissant 0/40 0/40 ″

Experimental Example 4 Test of Antioxidant Effect by DPPH Scavenging Activity According to Concentration of Grain Fermented Extract Fermented with Brewer's Yeast

Skin aging processes with free radicals include lipid peroxidation, protein oxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal crosslinks. Most of these reactions are radical reactions that involve an automatic oxidation process. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the ability to give electrons, that is, the greater the reducing power, the stronger the antioxidant. One reagent that can measure the reducing power of antioxidant is 1,1-diphenyl-2-picrylhydrazyl radical (DPPH). DPPH exists as a radical with a stabilized structure at the nitrogen center in the compound. Maximum absorption at 517 nm is shown and when reduced, absorption is lost at 517 nm.

The magnitude of the reducing power of the sample is expressed by the radical scavenging activity (SC ₅₀ ), SC ₅₀ is represented by the concentration of the sample required to reduce the concentration of DPPH by 50%.

The antioxidant effect of DPPH scavenging activity of the grain fermented extract fermented with brewer's yeast prepared by the method of Example 1 in the present invention was measured and shown in the following [Table 4].

Content of Example 1 (μg / mL) DPPH Scavenging Activity (%) 12.5 5 25 8 50 27 100 39 200 42

Experimental Example 5: Inhibitory Effect of Tyrosinase Activity of Grain Fermented Extract Fermented with Brewer's Yeast

In the present invention, the effect of inhibiting tyrosinase activity was measured for the grain fermented extract fermented with brewer's yeast obtained in Example 1, and arbutin was used as a comparative substance.

First, make tyrosine in 0.3mg / ml solution in distilled water, add 1ml of the solution to the test tube, and add 1ml of potassium phosphate buffer solution (0.1M, pH 6.8) and concentration (25, 50, 100 (%, W / V)). ) 0.9ml of the sample was added and reacted in a 37 ° C. thermostat for 10 minutes. At this time, 0.9ml of the solvent was added instead of each sample as a control. 0.1ml each of 2500Unit / ml tyrosinase solution was added to the reaction solution and reacted for 10 minutes in a 37 ° C thermostat.

The test tube containing the reaction solution was placed in iced water, quenched to stop the reaction, and the absorbance at 475 nm was measured with a photoelectron spectrometer. The tyrosinase activity inhibitory effect of each sample was calculated | required by the following formula.

Tyrosinase activity inhibitory effect (%) = 100- (reaction absorbance of each sample / control absorbance of control) × 100

Experimental results were confirmed to inhibit tyrosinase activity in accordance with the concentration of the fermented cereal grains fermented with brewer's yeast as shown in the following [Table 5].

Concentration of Example 1 (vol%) Tyrosinase activity inhibitory activity (%) 25 8 50 28 100 45

Experimental Example 6: Melanin synthesis inhibitory effect according to the concentration of the fermented cereal grains fermented with brewer's yeast of the present invention

Melanocytes are differentiated cells in the basal layer of skin epithelial tissue, which have specialized organs called melanin bodies that synthesize melanin. Melanin is known to play an important role in pigmentation such as blemishes and freckles, as well as protecting skin from UV rays and acting as a scavenger of cytotoxic radicals. Currently, in vitro tyrosinase activity inhibition test and intracellular melanogenesis inhibition test are most commonly used to evaluate the efficacy of whitening raw materials. This experiment is to evaluate the efficacy of the extract as a whitening raw material through the melanin synthesis inhibition experiment in B16-F1 cells are shown in the following [Table 6].

Content of Example 1 (μg / mL) Melanin content (%) Control 100 10 97 50 93 100 90

Claims (3)

Grain mixture consisting of malt, rice bran, and wheat is immersed in water at 80 to 100 ° C, aged for 30 minutes to 1 hour, and saccharified to obtain wort, and the fermentation broth obtained by condensing the wort with beer yeast is concentrated and dried. The fermented extract is obtained in powder form, and the grain fermented extract powder is added to a mixed solution composed of purified water and 1.3-butylene glycol to form a suspension, and the solution is obtained by irradiating ultrasonic waves at a low temperature of 30 to 60 ° C. and then filtering. A whitening cosmetic composition using brewer's yeast containing grain fermented extract fermented with brewer's yeast.
The method of claim 1,
The grain mixture is composed of 40 to 70% by weight of malt, 15 to 30% by weight of rice bran, 15 to 30% by weight of wheat, and the grain fermentation extract contains 0.01 to 10% by weight of the total weight of the whitening cosmetic composition. A whitening cosmetic composition using brewer's yeast containing grain fermented extract fermented with brewer's yeast.
The method of claim 1,
Grain fermented extract fermented with beer yeast is characterized in that the fermented extract fermented with brewer's yeast is added to the formulation selected from softening water, astringent makeup, nutritional cream, nutrition cream, body lotion, body oil, body essence, cleanser, pack Whitening cosmetic composition using brewer's yeast containing.