KR20120032690A - Cosmetic composition with the immunoprotective effect - Google Patents

Abstract

PURPOSE: A cosmetic composition containing mushroom is provided to ensure immunity, antigaing, and anti-wrinkling effect. CONSTITUTION: A cosmetic composition contains 2-10 wt% of mushroom extract as an active ingredient. A method for preparing the mushroom extract comprises: a step of washing mushrooms with purified water; a step of pulverizing the mushrooms and dipping in purified water or mixture of purified water and ethanol; a step of sonicating at low temperature and at 20 kHz; and a step of concentrating the extract. The cosmetic composition is manufactured in the form of a facial essence(mask), nutrition lotion, sun block cream, body lotion, and body essence.

Description

Anti-aging cosmetic composition with immune effect {Cosmetic composition with the immunoprotective effect}

The present invention relates to an anti-aging cosmetic composition by the immune effect,

More specifically, it provides a cosmetic composition with excellent anti-aging effect that contains Mont Blanc mushrooms, and makes the skin soft and shiny through various immune effects, skin aging, and prevention of wrinkles. Extracting abundant and useful active ingredients from mushrooms consisting of mushrooms and ganoderma lucidum is to provide a cosmetic composition containing the extract consisting of them.

The main symptoms of skin aging are wrinkles and the mechanism of their occurrence is not known. The main cause of damage to matrix proteins such as collagen and elastic fibers of skin is expected to be able to develop new ways to prevent and treat wrinkles. Therefore, in order to evaluate the effect of the functional material wrinkle improvement Wrinkle-related efficacy test is possible by measuring the intracellular collagen production test and collagenase activity inhibitory ability. In recent years, attention has been focused on aging-related diseases, as aging of muscles and skeletons, as well as aging of the skin, is a process that directly affects the daily lives, psychological and social well-being of individuals. It is the body's primary line of defense against physical harm, and it also plays an important role in regulating body temperature.

Recently, the anti-cancer activity of mushrooms is known, as well as food, as well as the material of the drug, the physiological activity of the mushroom is not limited to the anti-cancer effect, and has an effect on various adult diseases such as blood pressure, diabetes. The pharmacological efficacy of mushrooms is mainly due to the various kinds of polysaccharides (betaglucans) contained in mushrooms, and their activity does not affect normal cells and shows indirect activity by immunological activity, and therefore not only cancer but also various modern diseases. As it is associated with immunity, its importance is increasing. Mushroom polysaccharides show a significant difference in the constituents of polysaccharides depending on the composition of the medium, especially protein polysaccharides, because the amino acid composition of the protein varies depending on the culture medium. If the composition of the polysaccharide changes depending on the composition of the medium, there will be a difference in the chemical composition of the general organic matter and also in the components that exhibit physiological activity. Research institutes have been separating and developing substances with whitening effects from mushrooms registered in seed (breed protection No. 1430), which have not only immunomodulatory substances but also high-efficiency anti-aging, cholesterol lowering, and arthritis. It has been found that it contains pharmacological substances with various effects, such as alleviation. Especially, immunomodulatory substances have anti-inflammatory effects as well as their own immunomodulatory effects. Therefore, it is possible to extract developmental materials in addition to anti-aging function of skin through immunoregulation, as well as various pharmacological functions such as whitening, anti-degenerative, anti-aging, and anti-cancer. It is expected to be developed as a complex functional natural material that gives bioactive function.

The sample mushroom to be used in the study of the present invention contains various inhibitory functions against aging, and when it is developed as a cosmetic material, it has a function of inhibiting the aging process of other organs or tissues of the human body as well as a direct inhibitory or alleviating effect on skin aging. It can be given that it is possible to suppress or alleviate aging of the skin through aging inhibition by a complex function including regulation of gene expression. The anticipated role of mushrooms in the cosmetics material industry is not only substances that suppress the aging of the skin by strengthening the immune function of the skin, but also natural materials with various pharmacological effects that have high blood pressure, anti-hyperlipidemia, immunomodulatory function, fatty liver inhibition and arthritis prevention effect. It has been found that the substance contains a large amount in the mushroom to be used as a sample of the present invention, but the fruiting body, which is an edible part, has a high price and the quality of the product and the homeostasis of pharmacological components are different for each cultivation unit. As in the case of other mushrooms, the economy is low. In particular, in order to germinate mushroom spawn and cultivate into an edible fruiting body, it takes a long time more than 60 days, which is an obstacle to smooth supply and demand of raw materials. However, in the case of mass production by liquid cultivation through spawn fermentation of sample mushrooms, it is possible to mass-produce by developing customized culture method with optimal production conditions, and to produce high quality raw materials consistently throughout the year without time and space constraints. Free control of type and content can maximize the economics required for industrialization of natural materials with immune enhancement function and skin aging prevention function by reducing cost by improving production technology.

The present invention is to solve the problems as described above, as a part of the research to overcome the disadvantages of the cosmetics already known and to develop an anti-aging cosmetics by more immune effect mushroom seed liquid of mushrooms excellent production of mushroom mycelium from various mushrooms As a result of considering the characteristics of these extracts, cosmetics containing complex extracts consisting of Mont Blanc mushrooms, situation mushrooms, chaga mushrooms, and ganoderma lucidum mushrooms in terms of anti-aging can enhance the immune effect of the skin. It aims at providing a composition.

The cosmetics including the mushroom extract prepared by the extraction method extracted using the ultrasonic wave of the present invention through the above Examples and Experimental Examples relates to a cosmetic composition having a skin protection effect and a moisturizing elasticity function against ultraviolet rays, and more specifically, Extracts consisting of Mont Blanc mushrooms, Situation mushrooms, chaga mushrooms, and Ganoderma lucidum mushrooms have excellent ultraviolet absorption effect, have a high non-absorption effect, and have various bases and additives for cosmetics when compared to hot water extraction and solvent extraction using ultrasonic extraction method. Solubility, affinity, and stability in organic solvents, etc. are good, and especially when used in combination with the existing sunscreen has a synergistic effect of significantly increasing the sunscreen index.

1 is a graph showing the inhibitory effect of tyrosinase activity of the mushroom extract prepared by the present invention,
Figure 2 is a graph showing the antioxidant effect by the DPPH scavenging ability of the mushroom extract prepared by the present invention,
Figure 3 is a graph showing the antioxidant effect by the superoxide anion elimination activity of the mushroom extract prepared by the present invention,
Figure 4 is a graph showing the antioxidant effect of the hydroxyl radical (hydroxyl radical) scavenging activity of the mushroom extract prepared by the present invention,
5 is a graph showing the anti-inflammatory effect according to the inhibitory ability of lipoxygenase of the mushroom extract prepared by the present invention

The present invention relates to a cosmetic composition composed of a mushroom extract consisting of a Mont Blanc mushroom, a situation mushroom, chaga mushroom, Ganoderma lucidum as an anti-aging cosmetic composition by the immune effect, and more particularly, each of the mushroom extracts The extracts were extracted by the ultrasonic extraction method, and thus the extraction process was improved by the ultrasonic extraction method. The extracts obtained by the ultrasonic extraction method had excellent antioxidant, whitening and anti-inflammatory effects when compared with the extract extracted by the hot water extraction method and the solvent extraction method. It has high specific absorbency, good solubility, affinity and stability for various bases, additives and organic solvents for cosmetics, and especially when used in combination with existing sunscreens, it provides a synergistic effect to significantly increase the sunscreen index from various stresses. Protect your skin to moisturize and clean It is to provide a cosmetic composition excellent in cultivating effect.

The preparation method of the mushroom extract of the present invention uses a low-temperature ultrasonic extraction method which improves the extraction efficiency of the extraction by immersion, and in more detail, the ultrasonic extraction method used in the present invention is pulverized, mixed and extracted As a method applied in order to obtain good efficiency in a fast reaction time in the process, the conventional extraction method, the hot water extraction method has a lower extraction efficiency, more energy consumption and a lot of useful components due to heat than the ultrasonic extraction method used in the present invention It has the disadvantages of destruction and loss, heat denaturation of proteins, and unstable heat. On the other hand, the ultrasonic extraction method has the advantage of increasing the efficiency of the extraction process and safely and effectively obtaining many bioactive substances when applied to herbs or natural herbal raw materials.In the ultrasonic extraction method, ultrasonic waves are sound (sound waves) and the human ear. Refers to the sound of high frequency which cannot be heard, where low temperature means below 60 ℃, and the situation mushrooms are generally designed to avoid the extraction temperature of high or cryogenic level to selectively remove unnecessary components and It was aimed at selective reinforcement and overcomes the disadvantages caused by inadequate selectivity of solvent by ultrasonic extraction method. In other words, efforts have been made to minimize the effect on the compositional changes of the mushroom extracts that may occur during extraction, and this ultrasonic extraction method stabilizes the unstable structure of various useful components, and penetrates deeply into plant raw materials by various ranges of ultrasonic waves to enable effective ingredients of natural products. It has the advantage that it can extract effectively.

Advantages of the ultrasonic extraction method used in the preparation of the mushroom extract of the present invention has the feature of controlling the intensity of the ultrasonic energy change depending on the raw material to be extracted. Therefore, the extraction efficiency is very high depending on the raw materials, and the possibility of denaturation and destruction of active ingredients exhibiting pharmacological activity is minimized, and the extraction time is shortened because the production speed is faster than that of the conventional hydrothermal extraction method, which results in low cost and low energy use. It was confirmed to have.

The extract used in the mushroom extract of the present invention is composed of an extract consisting of Mont Blanc mushroom, situation mushroom, chaga mushroom, Ganoderma lucidum mushroom.

The raw material of each extract used in the preparation of the mushroom extract of the present invention is the raw material is first washed with purified water, and then finely pulverized, 1 to 10% by weight of the pulverized product in addition to purified water, polyol or ethanol, respectively 1 to 50% by weight of their mixed solvent The active ingredient of the mushroom extract may be modified by dividing into a step of immersing in% for 1 to 5 days preferably 3 days and using a low temperature ultrasonic extraction method made for 1 to 5 hours. At this time, by selecting one of polyol, ethanol, or a mixed solvent thereof, 10 to 70% by weight, 10 to 40% by weight of purified water may be mixed and used as a mixed solvent to be extracted by ultrasonic method.

That is, the immersion step and the ultrasonic wave using a polyol or ethanol, or a mixed solvent thereof, in addition to purified water, as an extraction solvent for the mushroom-based raw material consisting of Mont Blanc mushrooms, situation mushrooms, chaga mushrooms, and ganoderma lucidum constituting the mushroom extract of the present invention. The extraction step is carried out and finally extracted, and each extract is mixed and used. Therefore, the sedimentation reaction due to interference by different raw materials is prevented in the extraction step of each subdivided raw materials.

One or more of hexylene glycol, dipropylene glycol, and propylene glycol can be selected and used as a polyol from the said extraction solvent.

An organic solvent or purified water containing 1 to 5 times ethanol or butylene glycol is added to the mushroom extract mixed at the above compounding ratio and added during the preparation of the cosmetic.

When the mushroom extract according to the present invention is used in the cosmetic composition, it is characterized in that it contains 0.1 to 50% by weight, preferably 2 to 10% by weight.

At this time, less than 0.1% by weight of the skin protection effect and moisturizing elasticity of the present invention is insufficient when added, and when used more than 50% by weight of the unique flavor and color according to the unique flavor in the final product is strong Difficulty in product application, more preferably limited to 2 to 10% by weight.

Cosmetic composition having a skin calming effect and moisturizing elasticity against ultraviolet rays according to the present invention may have a conventional formulation, for example, supple cosmetics, facial essence (mask), nutrition lotion, nutrition cream, sunblock cream, body Lotions, body essences, and the like. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Hereinafter, a representative facial essence and a nourishing cream composition of a cosmetic composition having a skin protection effect against ultraviolet rays and a moisturizing elasticity function will be described as an example.

In addition, the mushroom extract excellent in the antioxidant effect, the whitening effect and the anti-inflammatory effect according to the present invention can be used according to a conventional method of use, the number of times of use may vary depending on the user's skin condition or taste.

Hereinafter, the cosmetic composition containing the mushroom extract excellent in the antioxidant effect, the whitening effect, and the anti-inflammatory effect of the present invention will be described in more detail with reference to the examples, but the present invention is not limited to these examples.

Example 1 Preparation of Mushroom Extract

Extracts consisting of Mont Blanc mushrooms, mushrooms, chaga, and Ganoderma lucidum were used to dry each raw material.

Mont Blanc mushroom, sang mushroom, chaga mushroom, ganoderma lucidum raw material constituting the mushroom extract of the present invention is washed with purified water firstly, each raw material, and then finely crushed, and grouped raw materials for each group based on the ground solids Maximize the extraction of the active ingredient of the extract for each raw material using the ultrasonic extraction method at 25 ℃ of low temperature for 3 hours by immersing in 20g / L level of purified water for 1 hour using 1 to 10% by weight of the extraction solvent It was.

That is to say, one of the polyol, ethanol or polyol and ethanol mixed solvent as the extraction solvent for the mushroom-based raw material consisting of Mont Blanc mushroom, sang mushroom, chaga mushroom, Ganoderma lucidum constituting the mushroom extract of the present invention and purified water and The mixture is mixed at a predetermined ratio, followed by an immersion step and an ultrasonic extraction step to finally extract and mix the extract of each raw material and apply the concentration step. Thus, in order to prevent the precipitation reaction caused by the interference by different raw materials in the extraction step of each subdivided raw materials.

As a mushroom extract excellent in antioxidant, whitening and anti-inflammatory effects according to the present invention, as a manufacturing method of the extract consisting of Mont Blanc mushroom, S. mushroom, chaga, Ganoderma lucidum mushroom, the purified water is selected as the extraction solvent and extracted for the complex sample Extraction experiments were performed at room temperature using the ultrasonic power and extraction time per unit volume of the extraction solution as process and system variables with a constant solvent ratio. The ultrasonic generator used a high power operating at 20 kHz. Powers of 400, 1200, 2400, and 4000 W / L were applied.

In the method of producing a mushroom extract excellent in the antioxidant effect, the whitening effect and the anti-inflammatory effect according to the present invention, the extraction effect of the pharmacologically active ingredient with the beta glucan as an indicator according to the ultrasonic treatment time was weak when using the low power ultrasonic generator. In the preparation method of the mushroom extract excellent in the antioxidant effect, the whitening effect and the anti-inflammatory effect according to the present invention, the output of 2400W / L for the high power was applied. As a reason for this, when the 3 hours high-power ultrasonic treatment was performed, the yield of the mushroom extract of the present invention was not remarkably decreased in the output of 1100 to 2400 W / L, and the yield of the mushroom extract was the best. Above 2400 W / L, the extraction yield did not increase significantly. Therefore, in the preparation method of the mushroom extract according to the present invention was treated with high power ultrasound of 3 hours was able to obtain the best effective yield at the output of 1200 ~ 2400W / L. In addition, the effective yield decreased as the ratio of purified water increased as the extraction solvent. That is, when using purified water as the extraction solvent of the mushroom extract according to the present invention, the titration range exceeds the range of 10, 20, 30, 50, 70, 100 g / L (mushroom mixture solid content / purified water ratio) in the range of 50 g / L Mask effective yield was confirmed to be lowered to 60% level of 20g / L. Therefore, when using purified water as the extraction solvent, the effective range was found to be the most suitable 20 ~ 50g / L. In this case, when the purified water was used at less than 20 g / L, the amount of the mushroom mixture sample was excessively added per unit volume of the extraction solvent, thereby confirming that the concentration effect of the mushroom mixture extract was greatly reduced.

As shown in Table 1 below, the raw materials consisting of Mont Blanc mushrooms, situation mushrooms, chaga mushrooms, Ganoderma lucidum mushrooms were extracted after low-temperature ultrasonication, and collected by collecting all the raw material filtrate extracts to form a final mixed extract, which was concentrated under reduced pressure. To obtain a mushroom extract consisting of 2g Mont Blanc mushroom, situation mushroom, chaga mushroom, Ganoderma lucidum mushroom.

Weight before extraction (g) Weight after extraction (g) 10.0 2.0

Method for producing a mushroom extract according to the present invention, when extracting a physiologically active substance from natural plants, such as Mont Blanc mushrooms, situation mushrooms, chaga mushrooms, ganoderma lucidum, if a little ultrasonic wave is applied, even if only a few minutes at room temperature to react the organic solvent Preliminary results have been obtained that the extraction effect is superior to that extracted by using the extract for 30 minutes at 60 ℃. However, a control group was prepared as a hot water extraction method for more objective evaluation in the method for preparing an extract consisting of Mont Blanc mushrooms, S. mushrooms, Chaga mushrooms and Ganoderma lucidum according to the present invention. That is, as a control for the mushroom extract according to the present invention, the raw material 10g was first washed with purified water, and then finely pulverized and 1 to 10% by weight of the ground product was immersed in purified water for 1 hour, and purified water was used as an extraction solvent for 3 hours. A sample of 2 g of control was prepared using hot water extraction as the 100 ° C. extraction temperature.

Example 2 Preparation of Facial Essence with Skin Protection and Elasticity Effect Containing Mushroom Extract According to the Present Invention

To prepare a facial essence in the composition ratio as shown in Table 2. The manufacturing method includes the steps of adding and dissolving the purified water portion to 60 ° C purified water, adding and dissolving the moisturizing elastic component dissolved at 40 ° C, and cooling and then adding and dissolving the functional cosmetic ingredient at 30 ° C. And after aging prepared by mixing with the mushroom extract prepared in Example 1 to prepare a facial essence.

Classification Raw material name Weight (% by weight) Purified water Purified water 65.53 glycerin 7.0 Dipotassium glycyrinate 0.1 Disodium ID 0.05 Hydroxyethyl Cellulose 0.05 Dipropylene glycol 3.0 Moisturizing elasticity part Methylparaben 0.1 Eichparaben 0.1 Tocopherol Acetate 0.02 Squalane 0.05 Polyoxyethylene HydroxyCaster Oil 1.1 Sodium Pie 1.0 Functional material part Witch Hazel Extract 1.0 Sodium hyaluronate 0.5 Collagen 0.2 Aloe extract 0.2 Mushroom extract 20.0 Sum 100.00

Example 3 Preparation of Nutritional Cream Having Skin Protection Effect and Whitening Function Containing Mushroom Extract According to the Present Invention

Dissolving the purified water portion by heating it to 80 ° C., emulsifying by adding the dissolved oily part heated to 80 ° C., adding and mixing a thickener dissolved in warm water at 60 ° C., and cooling and adding and mixing cosmetic ingredients. Cooling to 30 ℃ and defoaming, performing the step of filtration and aging prepared at 50 ℃ to prepare a nourishing cream.

In addition, when the mushroom extract is used in the cosmetic composition, the prepared mushroom extract is characterized in that it contains 0.1 to 50% by weight, preferably 2 to 10% by weight.

Classification Raw material name Weight (% by weight) Purified water Purified water 52.36 Propylene glycol 5.0 glycerin 6.0 Xanthan Gum 0.1 Triethanolamine 0.7 Methylparaben 0.2 Phenoxyethanol 0.1 Mushroom extract 10.0 Oily ingredients Squalane 8.0 Cetyl alcohol 2.2 Cyclopentasiloxane 2.0 Stearic acid 1.5 Glyceryl Monostearate 1.0 Microcrystalline Wax 0.6 Fiji-100 0.3 Tocopherol Acetate 1.0 Shea butter 1.0 Propylparaben 0.1 Oryzanol 0.1 Bisabolol 0.2 Dimethicone 0.5 Monostearic acid polyoxyethylene sorbitan 0.8 Thickener Carbomer 0.24 Purified water 2.4 Beauty ingredients Sodium ascorbate 0.1 Phospholipids 1.0 Placenta Extract 0.5 Aloe extract 0.5 Tocopherol Acetate 0.5 Apple extract 1.0 Sum 100.00

At this time, when less than 0.1% by weight, the antioxidant effect, the whitening effect, and the anti-inflammatory effect of the present invention are insignificant, and when used more than 50% by weight, the unique product and strong odor according to the intrinsic flavor in the final product are strongly applied. There is a difficulty in more preferably limited to 2 to 10% by weight.

Experimental Example 1: Confirmation of skin irritation, skin protection and elasticity effect of the facial essence containing the mushroom extract according to the present invention

30 minutes after applying the facial essence prepared in Example 2 (10m / 5cm2) to the forearm of 30 healthy men and women (15 males, 15 females), and shielding the other parts with aluminum foil Long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, it was confirmed that there was no phototoxicity and skin protection effect because no side effects such as erythema did not occur on the skin, and 60 healthy men and women (30 males and 30 females) were divided into two groups. After 1 hour, 3 hours, 6 hours as a control to prepare Example 2 without containing the mushroom extract according to the present invention and the experimental group coated by a predetermined amount (10m / 5cm2) prepared facial essence 10mm diameter border As a result of comparing the time when the mark disappeared by giving a force of about 5 Newtons to the hollow cylinder consisting of the facial essence containing the mushroom extract of the present invention, it was found to disappear from the experimental group at about 20% level for each time compared to the control group. It was confirmed that there is an effect of improving skin elasticity by use.

Experimental Example 2: Confirm the skin irritation and protective effect of the nutrient cream containing the mushroom extract according to the present invention

30 minutes after applying the nutrition cream prepared in Example 3 (10m / 5cm2) to the forearm of 30 healthy men and women (15 males and 15 females), and shielding the other parts with aluminum foil Long-wavelength ultraviolet rays were irradiated at a dose of 10.2 J / cm 2 using an ultraviolet irradiator, and skin conditions (erythema, edema, papules, etc.) were observed after 24 hours. As a result, no side effects such as erythema occurred on the skin, and thus the nourishing cream had no phototoxicity and had a protective effect on the skin. In addition, a healthy adult 60 men and women (30 males, 30 females) divided into two groups containing the experimental group applied the nutritional cream prepared in Example 3 by a predetermined amount (10m / 5cm2) and the mushroom extract according to the present invention 1 hour, 3 hours, and 6 hours after the preparation of Example 3, the skin elasticity was a hollow cylinder consisting of a 10 mm diameter border, and the strength of about 5 Newtons was applied to determine the disappearance time. Compared to the disappearance of the experimental group at the level of about 17% for each time, it was confirmed that the skin elasticity is improved by the use of the nutrient cream containing the mushroom extract of the present invention.

Experimental Example 3: Skin stability confirmation test of facial essence and nutrition cream according to the present invention

A human patch test was carried out on the forearm of 30 healthy adult men and women (15 males and 15 females) using the facial essences and nutrition creams prepared in Examples 2 and 3 above. Wipe the forearm of the subject well with 70% ethanol, and then place a pin chamber containing 20 μl of the facial essence and nutrition cream prepared in Examples 2 and 3 on a conventional cosmetic base using an adhesive tape for 24 hours. Closed attachment. After 24 hours, the adhesive tape and the pin chamber were removed, and the sample remaining on the skin with a gauze was lightly removed, and then the skin condition was observed, and again after 24 hours. Experimental results As shown in Table 4, the human patch test to confirm the skin irritation of the facial essence and nutrient cream according to the present invention did not show a positive response was confirmed that there is no irritation to the human skin.

sample After 24 hours (n = 30) After 48 hours (n = 30) Remarks Facial essence 0/30 0/30 Number of reaction quantums / total number of targets Nutrition cream 0/30 0/30

Experimental Example 4: Inhibitory effect of tyrosinase activity according to the concentration of the mushroom extract of the present invention

In order to confirm the inhibitory effect of tyrosinase activity using the extract obtained in Example 1, it was measured by modifying the method described in the literature, such as No. JK, et al .; Life Science , 65, p241-246, 1999 ). A sample of the control group was prepared by using a hydrothermal extraction method using water heated at 100 ° C. as the same extraction solvent at the same time as a control group of the method of preparing the extract obtained in Example 1.

Figure 1 is a graph showing the inhibitory effect of tyrosinase activity of the mushroom extract prepared by the present invention, which will be described in more detail, first 0.3 mg / ㎖ tyrosine solution in 1 ml test tube, 0.1M potassium phosphate buffer 1 ml of the solution (pH 6.8) and 0.9 ml of the sample according to the concentration of the extract (0.5, 1.0, 2.0, 4.0 (%, V / V)) were added and reacted for 10 minutes in a 25 ° C thermostat. In this case, only 0.9 ml of the solvent was used instead of each sample as a control. 0.1 ml each of 2500 units / ml of tyrosinase enzyme was added to the reaction solution, and then reacted in a 25 ° C. thermostat for 10 minutes.

The test tube containing the reaction solution was placed in iced water, quenched to stop the reaction, and the absorbance at 475 nm was measured with a photoelectric spectrometer. As shown in FIG. 1, the inhibitory effect of tyrosinase activity of the samples was 12% and 25% for the hydrothermal extract at 2.0 and 4.0 (%, V / V), respectively, and 25% and 43% for the ultrasonic extract. In addition, it was confirmed that the extract extracted by using ultrasonic waves showed a higher effect than the inhibitory effect of tyrosinase activity of the extract by the hot water extraction method.

Experimental Example 5: Antioxidant Effect of Mushroom Extracts by DPPH Scavenging Activity

Samples of the control group and the experimental group of the mushroom extract was prepared in the same manner as in Experiment 4.

Skin aging processes with free radicals include lipid peroxidation, DNA oxidation, melanogenesis, cleavage of connective tissue and abnormal crosslinks. Most of these reactions are radical reactions that involve an automatic oxidation process. Antioxidants are responsible for blocking the automatic oxidation in vivo. The greater the reducing power as an electron-giving ability, the stronger the antioxidant.

A reagent that measures the reducing power of antioxidants is 1,1-diphenyl-2 picrylhydrazyl radical (DPPH). DPPH exists as a radical of a stabilized structure of the nitrogen center in the compound. Maximum absorption at 517 nm is shown and when reduced, absorption is lost at 517 nm. The magnitude of the reducing power of the mushroom extract of the present invention is represented by a radical scavenging activity (SCcavenging activity, SC50) and SC50 represents the concentration required to reduce the concentration of DPPH by 50%, Figure 2 is DPPH of the mushroom extract prepared by the present invention As a graph showing the antioxidant effect by the scavenging ability, it will be described in more detail, showing the results obtained by measuring the SC50 for each concentration of the mushroom extract, the concentration of the extract was 0.0004, 0.0008, 0.0017, 0.0033, 0.0067 (%, V / V) shows hot water extracts of 1%, 1%, 10%, 21%, 46%, and ultrasonic extracts of 9%, 9%, 19%, 43%, 78%. The extracts extracted using ultrasonic wave showed the antioxidant effect according to the higher DPPH scavenging ability, rather than the antioxidant effect of DPPH scavenging ability.

Experimental Example 6: Antioxidative Effect of Mushroom Extracts by Superoxide Anion Scavenging Activity

Samples of the control group and the experimental group of the mushroom extract was prepared in the same manner as in Experiment 4. Superoxide anion scavenging activity was measured by modifying Noro et al. using superoxide anion generation system by xanthine / xanthine oxidase enzyme reaction.

The change of absorbance due to oxidation of NBT due to superoxide anion produced by xathine / xanthine oxidase enzyme reaction was measured. Add 0.05 M Na2CO3, 3 mM xanthine, 3 mM EDTA, 0.15% BSA, 0.75 M NBT and sample, mix well and let stand at 25 ° C for 10 minutes, add xanthine oxidase and react for 20 minutes at 25 ° C, then 6 mM CuCl2 The reaction was stopped to measure the absorbance at 560 nm. Scavenging activity of superoxide anion was calculated according to the following equation.

* Superoxide anion scavenging activity (%) = [1- (AB) / (CB)] × 100

A: Absorbance of sample at 560 nm

B: Absorbance of blank at 560 nm

C: Absorbance of control at 560 nm

Figure 3 is a graph showing the antioxidant effect of the superoxide anion scavenging activity according to the concentration of the mushroom extract prepared by the present invention, if described in more detail,

At the concentrations of the mushroom extracts 0001, 0.002, 0.003, 0.007 (%, V / V), the hydrothermal extracts were 1%, 21%, 43%, 64%, and the ultrasonic extracts were 18%, 34%, 83%, 87% of the mushroom extracts from the hot water extraction method showed the antioxidant effect of the superoxide anion scavenging activity in the mushroom extract extracted using ultrasonic waves rather than the antioxidant effect of the superoxide anion scavenging activity.

Experimental Example 7: Antioxidative Effect of Mushroom Extracts by Hydroxy Radical Scavenging Activity

Samples of the control group and the experimental group of the mushroom extract was prepared in the same manner as in Experiment 4. Hydroxyl radicals are the most powerful free radicals that damage cells and cause free radical peroxidation of the skin cell membranes. In particular, hydroxy radicals and hydrogen peroxide are produced by the photosensitization reaction that begins with ultraviolet absorption, and they participate in the production of more dangerous hydroxyl radicals in vivo. That is, superoxide can reduce Fe (III) and Cu (II), and reduced Fe (II) and Cu (I) reduce hydrogen peroxide, resulting in hydroxyl radical. This can be assessed by measuring the ability to eliminate hydroxyl radicals generated by the Fenton reaction in vitro.

Figure 4 is a graph showing the antioxidant effect of the hydroxy radical scavenging activity of the mushroom extract prepared by the present invention, when described in more detail, the concentration of the mushroom extract 0.1, 0.2, 0.5 (%, V / V) The hot water extracts represent 12%, 27%, and 58%, and the ultrasonic extracts represent 42%, 49%, and 84%. Ultrasonic extracts use ultrasonic waves rather than the antioxidant effect of the hydroxy radical scavenging activity of the mushroom extracts. The extracts extracted from the extract showed antioxidant effect by higher hydroxy radical scavenging activity.

Experimental Example 8: anti-inflammatory effect of the mushroom extract according to the inhibitory ability of lipoxygenase

Samples of the control group and the experimental group of the mushroom extract was prepared in the same manner as in Experiment 4. Lipoxygenase (5-LO, 5-lipoxygenase) adds molecular oxygen to unsaturated fatty acids with 1,4-cis, cis-pentadiene (1,4-pentadiene) structures in the molecule, such as linoleic acid, linolenic acid, and arachidonic acid. It is an oxidase that produces 1,3-cis, trans-diene 5-hydroperoxide. It is the enzyme involved in the first reaction of leucotriene biosynthesis from arachidonic acid, which is involved in various inflammatory and allergic diseases. Therefore, anti-inflammatory effect can be evaluated by measuring inhibitory activity of lipoxygenase using SLO (soybean lipoxygenase) similar to 5-LO structurally and mechanism.

Figure 5 is a graph showing the anti-inflammatory effect according to the inhibitory ability of lipoxygenase of the mushroom extract prepared by the present invention, it will be described in more detail,

The concentrations of the extracts were 0.03, 0.05, 0.13, 0.25 (%, V / V), respectively, and the hydrothermal extracts represented 1%, 2%, 10%, and 22%, and the ultrasonic extracts were 4%, 8%, 19%, and 42%. The anti-inflammatory effect of the extract extracted by using ultrasonic wave was higher than the anti-inflammatory effect of lipoxygenase by the hot water extraction method.

As a result, the ultrasonic extraction method used in the preparation of the mushroom extract of the present invention has the advantage that the strength of the ultrasonic energy change can be adjusted according to the raw material to be extracted. Therefore, the extraction efficiency is very high depending on the raw materials, and the possibility of denaturation and destruction of the active ingredient exhibiting pharmacological activity is minimized, and the production speed is faster than that of the conventional hydrothermal extraction method, which shortens the extraction time and thus has the characteristics of low cost and low energy use. It was confirmed.

Cosmetic composition containing a mushroom extract excellent in antioxidant and whitening effect and anti-inflammatory effect according to the present invention may have a conventional formulation, for example, supple cosmetics, facial essence (mask), nutrition lotion, nutrition cream, sunblock It can be formulated into creams, body lotions, body essences and the like. The composition of each of these formulations may contain a variety of bases and additives necessary for the formulation of the formulation and are suitable, and the types and amounts of these components can be readily selected by those skilled in the art. Hereinafter, a representative facial essence and a nourishing cream composition of a cosmetic composition having a skin protection effect against ultraviolet rays and a moisturizing elasticity function will be described as an example.

In addition, the cosmetic composition containing the mushroom extract excellent in antioxidant and whitening effect and anti-inflammatory effect according to the present invention can be used according to a conventional method of use, the number of times of use may vary depending on the user's skin condition or taste.

While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, but on the contrary, ≪ RTI ID = 0.0 > and / or < / RTI >

Claims (2)

The raw materials consisting of Mont Blanc mushrooms, situation mushrooms, chaga mushrooms, and Ganoderma lucidum are first washed with purified water, and then each raw material is crushed finely, using 1 to 10% by weight of the extracted solvent on the basis of the solids of the powder, purified water or Mushroom extract obtained by soaking with purified solvent mixed with purified water and ethanol at 1:99 to 50:50 and ultrasonically extracting at a low temperature of 20 kHz at a power of 1200 to 2400 W / L
Cosmetic composition characterized in that it comprises 2 to 10% by weight of the mushroom extract of claim 1 as an active ingredient

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