KR102251306B1 - Cosmetic compositions containing Lactobacillus plantarum extract - Google Patents

Abstract

The present invention relates to a cosmetic composition comprising a Lactobacillus plantarum culture extract, and includes a Lactobacillus plantarum culture extract, a star anise extract, a virginia leaf extract, and an Indian goose berry extract, thereby improving skin wrinkles and whitening effects. And it has an effect of increasing the duration.

Description

Cosmetic compositions containing Lactobacillus plantarum extract

The present invention relates to a cosmetic composition comprising a culture extract of Lactobacillus plantarum , which has an effect of improving skin wrinkles, a whitening effect, and increasing the duration of moisture retention.

The skin is the largest organ in the body, always in direct contact with the external environment, and acts as a protective film to protect the living body from various stimuli or dry environments, and the generation and destruction of new cells occurs more actively than other organs.

In addition, it protects the body from physical abrasions, prevents moisture loss inside the body, protects against ultraviolet rays generated from the sun, and plays a very important role in regulating body temperature.

Such skin has problems such as contamination, dryness, troubles, and abnormal skin immune system due to various physical factors, external environmental factors, and infections.

In particular, with the rapid industrial development of the modern era, pollution problems such as air pollution and water pollution become serious, and the skin is exposed to external environments such as roughening, drying, aging, etc. due to the strong cooling and heating of the living space due to the improvement of the residential environment. Has been greatly influenced by

Human skin gradually becomes thinner due to intrinsic aging and photoaging, wrinkles increase, elasticity decreases, and spots and freckles increase due to UV exposure. In particular, endogenous aging occurs when the secretion of hormones decreases or the function and activity of immune cells decreases, resulting in decreased biosynthesis of bio-constituent proteins, which rapidly progresses as free radicals increase.

Humans constantly generate Reactive Oxygen Species in metabolic processes, which are known to act as substances that cause aging, either directly or indirectly. Various antioxidants are used in cosmetics to prevent skin aging. Representative synthetic antioxidants include butylated hydroxytoluene (BHT), butylated hydroxyanisole (BHA), and tertiary butylhydroquinone (TBHQ). In general, synthetic antioxidants are widely used due to their excellent effect and low price, but their use is limited because they are known to cause hepatomegaly, fat mutation and cancer as well as their own toxicity upon oral or skin contact.

Niacinamide and arbutin used as ingredients for whitening functional cosmetics, and retinol used as ingredients for wrinkle-improving functional cosmetics have a problem in that the whitening effect is degraded due to the restriction of the formulation amount due to component destruction by oxidation and skin irritation.

Recently, as various cosmetic compositions using natural substances appear on the market, consumers are interested in products using natural substances rather than products made of conventional chemical substances, and accordingly, cosmetic compositions using various natural substances are being manufactured.

In addition, the anti-wrinkle effect of cosmetics is based on the effect of improving the dermal layer by various active ingredients in addition to the temporary effect by moisturizing by moisturizing factors, and the growth of retinol, vitamin C, EGF, etc., which are the ingredients of representative wrinkle-improving cosmetics. It is well known that factor components exhibit wrinkle-improving efficacy by increasing collagen neoplasia from human dermal fibroblasts.

In order to prevent such phenomena and maintain healthier and more beautiful skin, physiologically active substances obtained from various known animals, plants, microorganisms, etc. are added to cosmetics to maintain the skin's intrinsic function and activate skin cells. Efforts have been made to effectively inhibit aging.

In particular, many anti-aging and anti-wrinkle products using plant ingredients have been developed. However, such cosmetic products based on plant extracts have the disadvantage of causing a foreign body sensation that becomes stiff around the eyes like applying glue during the evaporation of the solution when applied to the skin, and the average duration of the product effect is about 6-7 hours per day. In the case of outside activities all day, there is a problem that the persistence almost disappears in the end.

In the cosmetic field, research and development of functional cosmetics having effects such as whitening, wrinkle improvement effect, and duration increase are actively progressing, and in particular, research using natural substances is continuing to avoid toxic or irritating skin .

Republic of Korea Patent Publication No. 10-2019-0008509 Republic of Korea Patent Publication No. 10-2019-0094326

An object of the present invention is to provide a cosmetic composition comprising a culture extract of Lactobacillus plantarum , which has an effect of improving skin wrinkles, a whitening effect, and increasing the duration of moisture retention.

The cosmetic composition of the present invention for achieving the above object may include a Lactobacillus plantarum culture extract, a star anise extract, a virginia leaf extract, and an Indian goose berry extract.

The cosmetic composition may further include a mixed extract of mulberry bark and sky tari root.

The Lactobacillus plantarum culture extract may be an extract obtained by culturing Lactobacillus plantarum and disrupting cells with a homogenizer.

The star anise extract may be an extract obtained by fermenting star anise with Lactobacillus plantarum.

The mixed extract of the mulberry bark and the sky tree root may be an extract obtained by fermenting a mixture of the mulberry bark and the sky tree root with Lactobacillus plantarum.

The mulberry bark and the sky tarri root may be mixed in a weight ratio of 1: 0.2-0.8.

The mixed extract of the mulberry bark and the sky tari root may be mixed in an amount of 5 to 40 parts by weight based on 100 parts by weight of the Lactobacillus plantarum culture extract.

The cosmetic composition may be included in an amount of 5 to 40 parts by weight of star anise extract, 1 to 30 parts by weight of virginia prickly pear extract, and 1 to 20 parts by weight of Indian gooseberry extract based on 100 parts by weight of Lactobacillus plantarum culture extract.

The cosmetic composition is BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, sunscreen cream, moisture cream, hand cream, foundation, essence, nutrition Essence, mask pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion, and body cleanser.

The cosmetic composition may have an effect of improving skin wrinkles, a whitening effect, and an effect of increasing the moisture retention duration.

The cosmetic composition containing the cultured extract of Lactobacillus plantarum of the present invention is not toxic, and is effective in whitening skin, improving skin wrinkles, and increasing the duration of moisture retention.

In addition, the cosmetic composition of the present invention effectively inhibits the production of MMP-1 protein, promotes the production of type 1 procollagen, increases the ability to inhibit tyrosinase and melanin production. Excellent durability and adhesion.

The present invention relates to a cosmetic composition comprising a culture extract of Lactobacillus plantarum , which has an effect of improving skin wrinkles, a whitening effect, and increasing the duration of moisture retention.

Hereinafter, the present invention will be described in detail.

The cosmetic composition of the present invention comprises a Lactobacillus plantarum culture extract, a star anise extract, a virginia leaf extract and an Indian goose berry extract, and may further include a mixed extract of a mulberry bark and a sky tarri root.

The Lactobacillus plantarum (Lactobacillus plantarum) is a safe enough to produce metabolites useful to the human body and also used as a food ingredient. The Lactobacillus plantarum culture extract of the present invention is obtained by culturing the Lactobacillus plantarum and disrupting cells with a homogenizer, and contains metabolic components, cytoplasm, cell wall components, and polysaccharides of Lactobacillus plantarum. It is a fermented lysate.

The Lactobacillus plantarum culture extract improves wrinkles and skin tone and provides moisture and nutrition.

In addition, the star anise extract is a substance that is used with the Lactobacillus plantarum culture extract and the Virginia prickly pear extract to help whitening the skin and improve wrinkles, and is a fermented extract obtained by fermenting star anise fruit with Lactobacillus plantarum. . If the star anise extract is used as it is without fermentation with Lactobacillus plantarum, it is effective for skin whitening, but skin troubles and psoriasis may occur.

The star anise extract is used in 5 to 40 parts by weight, preferably 20 to 30 parts by weight, based on 100 parts by weight of the Lactobacillus plantarum culture extract. When the content of the star anise extract is less than the lower limit, even when used together with the Lactobacillus plantarum culture extract and the virginia leaf extract, there is no effect of whitening and wrinkle improvement, and when the content of the star anise extract exceeds the upper limit, adhesion may be lowered.

In addition, the virginia prickly pear extract is used with Lactobacillus plantarum culture extract, star anise extract, and a mixed extract of mulberry bark and sky tari root to improve wrinkles. It is a material that improves adhesion. When fermented with Lactobacillus plantarum, the Virginia Poongnyeonhwa leaf extract is preferably used without fermentation because its persistence and adhesion are lowered.

The Virginian algae leaf extract is used in an amount of 1 to 30 parts by weight, preferably 10 to 20 parts by weight, based on 100 parts by weight of the Lactobacillus plantarum culture extract. When the content of the Virginian prickly pear extract is less than the above lower limit, even when used together with the Lactobacillus plantarum culture extract, star anise extract, and a mixed extract of mulberry bark and sky tari root, wrinkles are not improved and persistence and adhesion may be reduced. If the upper limit is exceeded, skin itching may occur.

In addition, the Indian goose berry extract is used with the Lactobacillus plantarum culture extract to supply moisture to the skin and improve the whitening effect. When the Indian goose berry extract is fermented with Lactobacillus plantarum, the whitening effect may be lowered, so it is preferable to use it without fermentation.

The Indian gooseberry fruit extract is used in an amount of 1 to 20 parts by weight, preferably 5 to 10 parts by weight, based on 100 parts by weight of the Lactobacillus plantarum culture extract. When the content of the Indian gooseberry fruit extract is less than the lower limit, the amount of moisture supplied to the skin is small and the whitening effect may be lowered even when used with the Lactobacillus plantarum culture extract, and if it exceeds the upper limit, skin trouble may occur. have.

In addition, the mixed extract of the mulberry bark and the sky tarri root can be used with the Lactobacillus plantarum culture extract and the virginia leaf extract to keep moisture in the skin for a long time. It is a fermented extract obtained by fermenting a mixture of mulberry bark and sky tari root with Lactobacillus plantarum. When the mixture of the mulberry bark and the sky tari root is used as it is without fermentation with Lactobacillus plantarum, erythema may occur. Moreover, when the mulberry bark and the sky twig are fermented without mixing and then mixed and used later, the moisture retention duration and adhesion may be significantly lowered compared to the case of mixing and fermenting the two substances.

The mixture of the mulberry bark and the sky tarri root is a mixture of the mulberry bark and the sky tarri root in a weight ratio of 1:0.2-0.8, preferably 1:0.3-0.5. If the content of the roots of the mulberry bark is less than the lower limit, persistence and adhesion may be deteriorated, and if it exceeds the upper limit, dryness of the skin may be caused.

The mixed extract of the mulberry bark and the sky tari root is used in 5 to 40 parts by weight, preferably 15 to 25 parts by weight, based on 100 parts by weight of the Lactobacillus plantarum culture extract. If the content of the mixed extract is less than the above lower limit, it is difficult to exert a long-term effect even when used together with the Lactobacillus plantarum culture extract and the Virginia apex leaf extract, and persistence and adhesion may decrease, and if it exceeds the upper limit, adhesion decreases. It may cause skin itching.

In the present invention, the term "skin wrinkles" refers to fine lines caused by deterioration of the skin, and may be caused by genes, a decrease in collagen present in the skin dermis, an external environment, and the like. In the present invention, "skin wrinkle improvement" refers to suppressing or inhibiting the generation of wrinkles on the skin, or alleviating wrinkles that have already been generated.

The main function of skin fibroblasts is the regeneration of damaged skin tissue through the synthesis and proliferation of extracellular matrix.Exogenous aging factors such as photoaging or damage accumulation due to free oxygen, endogenous aging factors due to cell aging due to telomere disruption They degrade the physiologically active function of skin fibroblasts in the dermal layer. Type I collagen, which accounts for more than 95% of the skin's extracellular matrix and plays a very important part in the physiological activity of fibroblasts through interaction and signal exchange with cell adhesion molecules and cytoskeletons. When the synthesis of is reduced, the amount of collagen in the skin tissue is reduced, and accordingly, the surface tension is reduced, resulting in a decrease in skin elasticity and the formation of skin wrinkles, as well as a decrease in physiologically active functions including cell proliferation and regeneration It is the cause of the vicious cycle.

Therefore, it can be seen that collagen of skin fibroblasts is directly related to skin regeneration, skin elasticity, skin wrinkle formation, and tissue repair or regeneration in case of skin damage. That is, when the synthesis of collagen or procollagen in skin fibroblasts is promoted or the synthesis of matrix metallo-proteinase (MMP) that degrades collagen is inhibited, skin elasticity improvement, skin regeneration, Skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And it is possible to obtain effects such as skin aging prevention.

The composition comprising the culture extract of Lactobacillus plantarum of the present invention is characterized by increasing the expression of the type I procollagen protein and inhibiting the expression of the MMP-1 protein. Specifically, the composition containing the Lactobacillus plantarum culture extract of the present invention inhibits the expression of matrix metallo-proteinase, which promotes collagen degradation, while increasing the production of type I procollagen. In order to increase skin elasticity, skin regeneration, skin wrinkle improvement, wound healing, repair and regeneration of damaged skin tissue; And it may have an effect such as skin aging prevention, and the like. That is, it can have the effect of preventing or improving skin wrinkles.

In addition, in the present invention, the term "whitening" means that the skin is beautiful and white, and the skin color of a person is determined according to the concentration and distribution of melanin inside the skin.In addition to genetic factors, solar ultraviolet rays, fatigue, stress, etc. It is also affected by environmental or physiological conditions. Melanin is synthesized from melanocytes in the epidermal layer of the skin, and an enzyme called tyrosinase acts on tyrosine, a kind of amino acid, in the melanosome, an organelle within the melanocyte. ), and then made through a non-enzymatic oxidation reaction. When such melanin synthesis occurs excessively in the skin, it darkens the skin tone and causes spots, freckles, and the like. Therefore, by inhibiting the synthesis of melanin pigment by inhibiting the tyrosinase activity in the skin, not only can the skin tone be brightened to realize skin whitening, but also skin hyperpigmentation such as spots and freckles caused by ultraviolet rays, hormones and genetic causes. It can improve calmness.

In addition, the cosmetic composition of the present invention may further include water-soluble vitamins, fat-soluble vitamins, polymer peptides, polymer polysaccharides, sphingo lipids and seaweed extract.

The water-soluble vitamin may be any one that can be blended in cosmetics, but preferably vitamin B1, vitamin B2, vitamin B6, pyridoxine, pyridoxine hydrochloride, vitamin B12, pantothenic acid, nicotinic acid, nicotinic acid amide, folic acid, vitamin C, vitamin H And the like, and their salts (thiamine hydrochloride, sodium ascorbate, etc.) and derivatives (ascorbic acid-2-phosphate sodium salt, ascorbic acid-2-magnesium salt, etc.) can also be used in the present invention. Included in water-soluble vitamins. The water-soluble vitamin can be obtained by a conventional method such as a microbial transformation method, a microbial culture purification method, an enzyme method, or a chemical synthesis method.

The fat-soluble vitamins may be any as long as they can be formulated in cosmetics, but preferably include vitamin A, carotene, vitamin D2, vitamin D3, vitamin E, and their derivatives (ascorbine palmitate, ascorbate stearate). Bean, dipalmitate ascorbine, acetate dl-alpha tocopherol, nicotinic acid dl-alpha tocopherol vitamin E, DL-pantotenyl alcohol, D-pantotenyl alcohol, pantotenyl ethyl ether, etc.) are also included in the fat-soluble vitamins used in the present invention. do.

The polymeric peptide may be any one that can be blended in cosmetics, but preferably, collagen, hydrolyzed collagen, gelatin, elastin, hydrolyzed elastin, keratin, and the like may be mentioned. The polymeric peptide can be purified and obtained by a conventional method such as a microbial culture medium purification method, an enzyme method, or a chemical synthesis method, or can be used after being purified from natural products such as dermis of pigs and cattle, silk fibers of silkworms, and the like.

The polymer polysaccharide may be any one that can be blended in cosmetics, but preferably, hydroxyethyl cellulose, xanthan gum, chondroitin sulfuric acid or a salt thereof (such as sodium salt) may be used. For example, chondroitin sulfate or a salt thereof can be used after being purified from mammals or fish.

The sphingo lipid may be anything that can be blended in cosmetics, but preferably, ceramide, phytosphingosine, sphingosaccharide lipid, and the like may be mentioned. The sphingo lipid can usually be purified from mammals, fish, shellfish, yeast, plants, etc. by a conventional method or obtained by a chemical synthesis method.

The seaweed extract may be any one as long as it can be blended in cosmetics, but preferably, brown algae extract, red algae extract, green algae extract, etc. may be mentioned, and carrageenan, arginic acid, arginic acid purified from these seaweed extracts Sodium, potassium arginate, and the like are also included in the seaweed extract used in the present invention. Seaweed extract can be obtained by purifying from seaweed by a conventional method.

In the cosmetic composition of the present invention, in addition to the essential ingredients, other ingredients that are usually blended in cosmetics may be blended if necessary.

In addition, as a compounding component that may be added, a fat or oil component, a moisturizing agent, an emollient agent, an ultraviolet absorber, a pH adjuster, a fragrance, a blood circulation accelerator, a cooling agent, a restricting agent, and purified water can be mentioned.

Examples of the oil and fat components include ester oils, hydrocarbon oils, silicone oils, fluorine oils, animal oils, vegetable oils and the like.

The ester-based fats and oils include tri2-ethylhexanoate glyceryl, 2-ethylhexanoate, cetyl 2-ethylhexanoate, isopropyl myristate, butyl myristate, isopropyl palmitate, ethyl stearate, octyl palmitate, isocetyl isostearate, Butyl stearate, ethyl linoleate, isopropyl linoleate, ethyl oleate, isocetyl myristate, isostearyl myristate, isostearyl palmitate, octyldodecyl myristate, isocetyl isostearate, diethyl sebacate, Diisopropyl adipic acid, isoalkyl neopentanoate, tri(capryl, capric acid) glyceryl, tri2-ethylhexanoate trimethylolpropane, triisostearate trimethylolpropane, tetra2-ethylhexanoate pentaeli Slitol, cetyl caprylate, decyl laurate, hexyl laurate, decyl myristate, myristyl myristate, cetyl myristate, stearyl stearate, decyl oleate, ricinooleate cetyl, lauric acid Sourcetearyl, isotridecyl myristate, isocetyl palmitate, octyl stearate, isocetyl stearate, isodecyl oleate, octyldodecyl oleate, octyldodecyl linoleate, isostearate isopropyl, 2-ethylhexanoic acid tax Tostearyl, 2-ethylhexanoate stearyl, isostearate hexyl, ethylene glycol dioctanoate, ethylene glycol dioleate, propylene glycol dicaprylate, propylene glycol dicaprylate, neopentyl glycol dicaprylate, neopentyl dioctanoate Glycol, glyceryl tricaprylate, glyceryl triundecylate, glyceryl triisopalmitate, glyceryl triisostearate, octyldodecyl neopentanoate, isostearyl octanoate, octyl isononanoate, hexyl neodecanoate Decyl, octyldodecyl neodecanoate, isocetyl isostearate, isostearyl isostearate, octyldecyl isostearate, polyglycerololeic acid ester, polyglycerin isostearic acid ester, triisocetyl citrate, triisoalkyl citrate, Triisooctyl citrate, lauryl lactate, myristyl lactate, cetyl lactate, octyldecyl lactate, triethyl citrate, acetyltriethyl citrate, acetyltributyl citrate, trioctyl citrate, diisostearyl malate, hydroxystearic acid 2- Ethylhexyl, di2-ethylhexyl succinate, diisobutyl adipate, diisopropyl sebacate, dioctyl sebacate, cholesteryl stearate, isostear Acid cholesteryl, hydroxystearate cholesteryl, oleic acid cholesteryl, oleic acid dihydrocholesteryl, isostearate pitsteryl, pitsteryl oleate, 12-stealoylhydroxystearate isocetyl, 12 -Ester systems, such as stearyl hydroxy stearate stearyl and 12-stealoyl hydroxy stearate isostearyl, etc. are mentioned.

The hydrocarbon-based fats and oils may include hydrocarbon-based fats such as squalene, liquid paraffin, alpha-olefin oligomer, isoparaffin, ceresin, paraffin, liquid isoparaffin, polybuden, microcrystalline wax, and petrolatum.

The silicone-based fats and oils include polymethylsilicone, methylphenylsilicone, methylcyclopolysiloxane, octamethylpolysiloxane, decamethylpolysiloxane, dodecamethylcyclosiloxane, dimethylsiloxane methylcetyloxysiloxane copolymer, dimethylsiloxane methylsteaoxysiloxane copolymer, alkyl And modified silicone oil and amino-modified silicone oil.

Examples of the fluorine-based fats and oils include perfluoropolyether and the like.

The animal or plant fats include avocado oil, almond oil, olive oil, sesame oil, rice bran oil, soybean oil, corn oil, rapeseed oil, almond oil, palm kernel oil, palm oil, castor oil, sunflower oil, grape seed oil, cottonseed oil, palm oil, Cuckooinnut oil, wheat germ oil, rice germ oil, shea butter, walnut colostrum, marker demyanut oil, egg yolk oil, beef tallow, horse oil, mink oil, orange rape oil, jojoba oil, candelilla wax, carnauba wax, liquid ranol And animal or plant fats such as hydrogenated castor oil.

Examples of the moisturizing agent include a water-soluble low-molecular moisturizer, a fat-soluble molecular moisturizer, a water-soluble polymer, and a fat-soluble polymer.

The water-soluble low molecular weight moisturizers include serine, glutamine, sorbitol, mannitol, pyrrolidone-sodium carboxylic acid, glycerin, propylene glycol, 1,3-butylene glycol, ethylene glycol, polyethylene glycol B (polymerization degree n = 2 or more), Polypropylene glycol (polymerization degree n = 2 or more), polyglycerin B (polymerization degree n = 2 or more), lactic acid, lactate, and the like.

Examples of the fat-soluble low molecular weight moisturizer include cholesterol and cholesterol esters.

Examples of the water-soluble polymer include carboxyvinyl polymer, polyaspartic acid salt, tragacanth, xanthan gum, methylcellulose, hydroxymethylcellulose, hydroxyethylcellulose, hydroxypropylcellulose, carboxymethylcellulose, water-soluble chitin, chitosan, dextrin, etc. Can be mentioned.

Examples of the fat-soluble polymer include polyvinylpyrrolidone eicosene copolymer, polyvinylpyrrolidone hexadecene copolymer, nitrocellulose, dextrin fatty acid ester, and polymer silicone.

Examples of the emollient agent include long-chain acyl glutamate cholesteryl ester, hydroxystearate cholesteryl, 12-hydroxystearic acid, stearic acid, rosin acid, lanolin fatty acid cholesteryl ester, and the like.

Examples of the ultraviolet absorber include paraaminobenzoic acid, ethyl paraaminobenzoate, amyl paraaminobenzoate, octyl paraaminobenzoate, ethylene glycol salicylate, phenyl salicylate, octyl salicylate, benzyl salicate, butyl phenyl salicylate, homomentyl salicylate, Benzyl cinnamate, paramethoxycinnamic acid-2-ethoxyethyl, paramethoxycinnamic acid octyl, diparamethoxycinnamic acid mono-2-ethylhexane glyceryl, paramethoxycinnamic acid isopropyl, diisopropyl/diisopropyl cinnamic acid ester mixture , Urocanic acid, ethyl urocanate, hydroxymethoxybenzophenone, hydroxymethoxybenzophenonesulfonic acid and salts thereof, dihydroxymethoxybenzophenone, sodium dihydroxymethoxybenzophenone disulfonate, dihydroxy Benzophenone, tetrahydroxybenzophenone, 4-tert-butyl-4'-methoxydibenzoylmethane, 2,4,6-trianilino-p-(carbo-2'-ethylhexyl-1'-oxy) -1,3,5-triazine, 2-(2-hydroxy-5-methylphenyl)benzotriazole, etc. are mentioned.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmalic acid, sodium pmaate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Examples of the pH adjuster include citric acid, sodium citrate, malic acid, sodium malate, pmalic acid, sodium pmaate, succinic acid, sodium succinate, sodium hydroxide, sodium monohydrogen phosphate, and the like.

Cosmetics prepared by containing the composition of the present invention may take the shape of a solution, an emulsion, a viscous mixture, or the like.

In addition, the ingredients included in the cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions in addition to the above ingredients as an active ingredient. For example, conventional auxiliary agents such as stabilizers, pigments and natural perfumes, and It may further include a carrier.

The composition of the present invention can be variously used in cosmetics or face wash having an effect of improving skin wrinkles, whitening and increasing duration.

Products to which the composition of the present invention can be added include, for example, BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, UV protection There are cosmetics such as cream, moisture cream, hand cream, foundation, essence, nutrition essence, mask pack, and soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser.

When the formulation of the present invention is a paste, cream or gel, animal fiber, plant fiber, wax, paraffin, starch, tracant, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc, or zinc oxide may be used as a carrier component. I can.

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate, or polyamide powder may be used as a carrier component. In particular, in the case of a spray, additionally chlorofluorohydrocarbon, propane , Butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, a solvating agent or an emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylglycol oil, glycerol aliphatic ester, polyethylene glycol or fatty acid ester of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, an ethoxylated isostearyl alcohol, a suspending agent such as polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystalline Cellulose, aluminum metahydroxide, bentonite, agar or tracant, and the like may be used.

Hereinafter, a preferred embodiment is presented to aid in the understanding of the present invention, but it is obvious to those skilled in the art that various changes and modifications are possible within the scope of the present invention and the scope of the technical idea, but the following examples are only illustrative of the present invention, It is natural that such modifications and modifications fall within the scope of the appended claims.

Example 1.

A cosmetic composition was prepared, including 100 parts by weight of Lactobacillus plantarum culture extract, 23 parts by weight of star anise extract, 15 parts by weight of virginia leaf extract, and 8 parts by weight of Indian gooseberry extract.

The Lactobacillus plantarum culture extract is obtained by spreading the Lactobacillus plantarum on MSR agar, culturing the agar at 37° C., and crushing the cells with a homogenizer, and the star anise extract is Star anise 100 1 ml of Lactobacillus plantarum (Lactobacillus plantarum 10 ⁸ CFU/ml) was inoculated into ml, fermented at 37° C. for 2 days, and then 1 kg of purified water was added to the fermented product. It was obtained by ultrasonic extraction (Sonification) for minutes. In addition, 1 kg of purified water was added to each of the Virginian prickly pear leaves and Indian goose berry fruit extracts and obtained by ultrasonic extraction for 30 minutes using an ultrasonic extractor.

Example 2. Addition of mixed extract of mulberry bark and sky tari root

It was carried out in the same manner as in Example 1, but a cosmetic composition was prepared by adding a mixed extract of mulberry bark and sky tari root to the composition of Example 1 in an amount of 15 parts by weight.

The mixed extract of the mulberry bark and the sky tarri root was inoculated with 1 ml of ^{Lactobacillus plantarum (Lactobacillus plantarum 10 8} CFU/ml) to 100 ml of a mixture of the mulberry bark and the sky tarry root in a weight ratio of 1: 0.5 After fermentation at 37° C. for 2 days, 1 kg of purified water was added to the fermented product, followed by ultrasonic extraction for 30 minutes using an ultrasonic extractor.

Comparative Example 1. Star anise extract omitted

In the same manner as in Example 1, a cosmetic composition was prepared without using star anise extract.

Comparative Example 2. Star anise extract_fermentation omitted

In the same manner as in Example 1, a cosmetic composition was prepared using a non-fermented star anise extract instead of a star anise extract fermented with Lactobacillus plantarum.

The unfermented star anise extract was obtained by adding 1 kg of purified water to star anise and then ultrasonically extracting for 30 minutes using an ultrasonic extractor.

Comparative Example 3. Omitted extract of virginia prickly pear leaf extract

It was carried out in the same manner as in Example 1, but a cosmetic composition was prepared without the use of the extract of virginia prickly pear.

Comparative Example 4. Indian Gooseberry Fruit Extract Omitted

In the same manner as in Example 1, a cosmetic composition was prepared without using an Indian gooseberry fruit extract.

<Test Example>

All statistical analyzes were performed using SPSS version 18.0 (IBM, Chicago, IL, USA). The difference (p <0.05) of the mean values between the experimental groups was determined using a one-way ANOVA using Duncan's post-hoc test. Each experimental data was expressed as mean ㅁ standard deviation (SD).

Test Example 1. MTT assay

Cytotoxicity was measured using the MTT 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) reduction method.

The cultured HaCaT cells were well mixed in DMEM medium, ^{adjusted to 1×10 5} cells/mL, and then 1 mL of the prepared cells were added to a 24-well culture plate, followed by incubation for 3-4 days. When the cells grew about 80%, treated with various concentrations of samples (0, 25, 50, 100, 300, 400, 500, 1000 μg/mL) in a medium not added with fetal bovine serum and antibiotics, and then 24 hours. Further cultured and the survival rate of the cultured cells was measured using the MTT reduction method.

MTT solution (5 mg/mL) was added to each well, and about 1/10 of the volume of the growth medium was added, followed by further incubation at 37°C for 4 hours. And, taking care not to let the formazan generated by reducing MTT go out with the medium, remove the medium, leave it at room temperature for 30 minutes to completely remove the remaining medium, then dissolve the sample using DMSO, and then absorb the absorbance at 570 nm. Was measured. Cell viability was calculated as in Equation 1 below, and then shown in Table 1 below.

[Equation 1]

Cell viability (%) = [absorbance of sample treatment group/absorbance of control group] x 100

division Concentration (㎍/㎖) 10 100 No treatment 1001.1 Example 1 98.11.8 95.40.8 Example 2 97.72.1 94.31.5 Comparative Example 1 96.20.9 92.40.9 Comparative Example 2 96.81.4 91.91.1 Comparative Example 3 95.72.4 92.82.4 Comparative Example 4 96.11.1 90.91.8

As shown in Table 1 above, it was confirmed that not only the cosmetic compositions prepared according to Examples 1 and 2 of the present invention, but also the compositions of Comparative Examples 1 to 4 can be safely used for human skin.

Test Example 2. Wrinkle formation inhibition

2-1. Inhibition of synthesis of MMP-1 protein

CCD-986sk ^{was incubated at a concentration of 1.5×10 5} cells/ml in 35 mm dishes until reaching about 80% confluency. After removing the original medium, washing with PBS to remove the serum component in the medium, and then irradiating UVA (6. 3J/㎠) for 24 hours using a UV light source equipped with a filter (Coralife, 35W, UV).凋謝).

After UVA irradiation, samples of Examples or Comparative Examples of the present invention were administered at concentrations of 0.1, 10, and 100 ug/ml in DMEM medium to which FBS was not added, and then cultured for 24 hours. The cultured medium was dispensed into a 96 well plate and reacted at 4° C. overnight.

Measurement of the expression level of MMP-1 induced by UVA irradiation was performed using the method used by Dunsmore et al.

First, the medium was washed three times with TBS (phosphate bufferdsaline + 0.05% Tween20), and then blocked with 3% PBS at 37°C for 1 hour. Thereafter, a solution obtained by diluting the primary antibody (monoclonal anti-MMP-1 antibody) as a blocking buffer at a volume ratio of 1:3000 was added and reacted at 37° C. for 90 minutes. Then, after washing with PBS, a solution diluted with a blocking buffer (alkaline phosphatase conjugated Goat anti-mouse IgG) in a volume ratio of 1:3,000 was added and reacted at 37° C. for 90 minutes. Then, after washing with PBS, alkaline phosphatase substrate solution (1 mg/ml, p-mitrophenylphosphate in diethanolamine buffer) was added to react at room temperature for 30 minutes, and the reaction was stopped with 3N NaOH. Next, absorbance was measured at 405 nm using a microplate reader.

division Concentration (㎍/㎖) 10 100 No treatment 8781 Example 1 5915 4483 Example 2 5412 3881 Comparative Example 1 7256 7148 Comparative Example 2 7060 6819 Comparative Example 3 6900 6724 Comparative Example 4 6948 6649

As shown in Table 2 above, the cosmetic compositions prepared according to Examples 1 and 2 of the present invention effectively inhibit the production of MMP-1 protein compared to the untreated group and the compositions of Comparative Examples 1 to 4 to prevent skin wrinkles. And it was confirmed that it can be effectively used for improvement.

In particular, it was confirmed that the cosmetic composition of Example 2 more effectively inhibited the production of MMP-1 protein compared to the cosmetic composition of Example 1.

2-2. Promotes the synthesis of type 1 procollagen protein

In order to confirm the effect of improving skin wrinkles for the cosmetic compositions of Examples and Comparative Examples, it was intended to measure the amount of protein collagen produced that maintains the elasticity of the skin.

Fibroblasts were dispensed in a 48 well plate at ^{a density of 1×10 4} cells/well, and cultured in DMEM medium containing 10% FBS for 24 hours, and then samples of Examples or Comparative Examples were added at 50 and 100 μg/mL and no Incubated for an additional 24 hours in serum medium. After incubation, the supernatant of each well was collected and the amount of procollagen type I C-peptide (PICP) was measured using procollagen type I C-peptide EIA kit (MK101, Takara, Japan) and converted into ng/ml. , The amount of collagen synthesized thereby was measured.

division Concentration (㎍/㎖) 10 100 No treatment 40.1 Example 1 64.8 86.9 Example 2 71.8 90.4 Comparative Example 1 44.5 47.9 Comparative Example 2 49.7 58.2 Comparative Example 3 47.5 55.7 Comparative Example 4 50.6 61.8

In order to confirm the effect of improving skin wrinkles, the amount of protein collagen produced that maintains the elasticity of the skin was measured.

As shown in Table 3 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention promote the production of type 1 procollagen compared to the untreated group and the compositions of Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 2 promotes the production of type 1 procollagen compared to the cosmetic composition of Example 1.

That is, the cosmetic composition prepared according to Examples 1 and 2 of the present invention promotes the production of type 1 procollagen and effectively prevents wrinkles through the action of inhibiting the expression of the collagen-degrading enzyme MMP-1, It was confirmed that there is an effect of improving the generated wrinkles.

Test Example 3. Whitening effect

3-1. Tyrosinase inhibitory effect

For the cosmetic compositions of Examples and Comparative Examples, measurement of the ability to inhibit tyrosinase enzyme activity, which is a representative method of the whitening efficacy evaluation test, was performed as follows using the dopachrome method.

150 µl of mushroom tyrosinase-150 unit, 225 µl of 2.5 mM L-tyrosine, 225 µl of 0.4 M hepes buffer (■ 6.8); 300 µl of an ethanol solution or a sample (0.5, 1, and 5 mg/ml) solution was mixed, and the absorbance was measured at 475 nm before incubation and after incubation for 15 minutes, respectively. The ability to inhibit tyrosinase was calculated by Equation 2 below.

[Equation 2]

Tyrosinase inhibition (%) = (C-D)-(A-B)/(C-D) ㅧ 100

(A: absorbance after cultivation of the sample-containing solution, B: absorbance before cultivation of the sample-containing solution, C: absorbance before cultivation of the sample-free solution, D: absorbance after cultivation of the sample-free solution)

division Concentration (㎍/㎖) 10 100 No treatment 88.4 Example 1 55.5 68.6 Example 2 56.8 69.1 Comparative Example 1 76.1 89.4 Comparative Example 2 67.4 82.1 Comparative Example 3 71.9 86.3 Comparative Example 4 69.8 84.5

As shown in Table 4 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention had excellent tyrosinase inhibitory effects compared to the untreated group and the compositions of Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 1 and the cosmetic composition of Example 2 had similar tyrosinase inhibitory effects.

3-2. Melanin production inhibitory effect

B16F1 cells, which are mouse-derived melanocytes, produce melanin, and melanin is biosynthesized in melanosome, an organelle in melanocytes in the basal layer of the epidermis, which can be compared by measuring the absorbance in the visible light region.

After inoculation of B16F1 cells, the sample was treated for 3 days, the medium was removed, the cells were washed with PBS, and 1 ml of 1 N NaOH was added to each well, followed by stirring to melt the cell membrane to dissolve the melanin component. The amount of melanin produced was measured by measuring absorbance in the blue visible light region, which has the shortest wavelength among the public, at 400 nm.

The amount of melanin was measured by a method expressed in terms of absorbance per unit cell count (10 ⁶ cells), and the relative amount of melanin production relative to the control group (untreated group) was calculated as an inhibition rate (%), and the results are summarized in Table 5.

Classification (Unit: %) Concentration (㎍/㎖) 10 100 Example 1 56.8 54.7 Example 2 69.8 79.4 Comparative Example 1 39.5 42.8 Comparative Example 2 44.5 50.9 Comparative Example 3 40.7 43.5 Comparative Example 4 42.4 45.7

As shown in Table 5 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention showed superior melanin production inhibitory ability compared to the untreated group and the compositions of Comparative Examples 1 to 4.

In particular, it was confirmed that the cosmetic composition of Example 2 has a more excellent melanin production inhibitory effect than the cosmetic composition of Example 1.

Test Example 4. Moisture persistence, persistence without clumping, and adhesion

Twenty women between the ages of 20 and 40 were subject to the experiment, and the duration of the experiment was 5 days. BB creams were prepared using the cosmetic compositions of Examples and Comparative Examples, and then provided to a total of 20 test subjects, respectively, and scored for adhesion. In addition, the same test subjects were asked to use the cosmetic compositions of Examples and Comparative Examples alternately for 1 day, and after 4 days, a questionnaire survey was conducted on each of the moisture retention and non-clumping persistence.

As the cosmetic composition of the comparative example, the cosmetic compositions of Comparative Examples 2 and 4, which are effective in improving wrinkles and whitening among the Comparative Examples, were used.

Moisture retention

Classification (unit: person(%)) Concentration (㎍/㎖) 10 100 Example 1 Very good 5 (25%) 6 (30%) good 10 (50%) 11 (55%) usually 5 (25%) 3 (15%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 7 (35%) 8 (40%) good 12 (60%) 12 (60%) usually 1(5%) -(0%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good 2 (10%) 2 (10%) usually 6 (30%) 4 (20%) Bad 10 (50%) 11 (55%) Very bad 2 (10%) 3 (15%) Comparative Example 4 Very good -(0%) -(0%) good 3 (15%) 4 (20%) usually 7 (35%) 5 (25%) Bad 9 (45%) 10 (50%) Very bad 1(5%) 1(5%)

As shown in Table 6 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention have higher satisfaction with moisture retention compared to the compositions of Comparative Examples 2 and 4.

Long-lasting power that doesn't clump together

Classification (unit: person(%)) Concentration (㎍/㎖) 10 100 Example 1 Very good 5 (25%) 7 (35%) good 10 (50%) 11 (55%) usually 5 (25%) 2 (10%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 8 (40%) 8 (40%) good 9 (45%) 11 (55%) usually 3 (15%) 1(5%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good 4 (20%) 4 (20%) usually 10 (50%) 11 (55%) Bad 5 (25%) 3 (15%) Very bad 1(5%) 2 (10%) Comparative Example 4 Very good -(0%) -(0%) good 1(5%) 3 (15%) usually 11 (55%) 13 (65%) Bad 5 (25%) 3 (15%) Very bad 3 (15%) 1(5%)

As shown in Table 7 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention have higher satisfaction with the persistence without clumping for a long time compared to the compositions of Comparative Examples 2 and 4.

Adhesion

Classification (unit: person(%)) Concentration (㎍/㎖) 10 100 Example 1 Very good 7 (35%) 9 (45%) good 11 (55%) 11 (55%) usually 2 (10%) -(0%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Example 2 Very good 8 (40%) 11 (55%) good 10 (50%) 9 (45%) usually 2 (10%) -(0%) Bad -(0%) -(0%) Very bad -(0%) -(0%) Comparative Example 2 Very good -(0%) -(0%) good 3 (15%) 4 (20%) usually 11 (55%) 12 (60%) Bad 5 (25%) 3 (15%) Very bad 1(5%) 1(5%) Comparative Example 4 Very good -(0%) -(0%) good -(0%) 2 (10%) usually 7 (35%) 8 (40%) Bad 11 (55%) 9 (45%) Very bad 2 (10%) 1(5%)

As shown in Table 8 above, it was confirmed that the cosmetic compositions prepared according to Examples 1 and 2 of the present invention have higher satisfaction with the adhesion of the cream to the skin compared to the compositions of Comparative Examples 2 and 4.

An example of preparing a cosmetic composition suitable for applying the present invention will be presented.

Preparation Example 1: Lotion

An example of preparing a lotion using the cosmetic composition of Example 2 is shown in Table 9 below.

ingredient Content (% by weight) Composition of Example 2 5.0 glycerin 6.0 1,3-butylene glycol 3.0 PG1500 1.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Sodium hyaluronate 5.0 ethanol 10.0 Polysorbate 20 0.2 Preservatives, fragrances, and colors a very small amount Distilled water Balance Sum 100

Manufacturing example 2: lotion

Examples of the preparation of lotions using the cosmetic composition of Example 2 are shown in Table 10 below.

ingredient Content (% by weight) Composition of Example 2 3.0 Propylene glycol 6.0 glycerin 4.0 Triethanolamine 1.2 Tocopheryl acetate 3.0 Liquid paraffin 5.0 Squalane 3.0 Macadamia Nut Oil 2.0 Polysorbate 60 1.5 Sorbitansquioleate 1.0 Carboxyvinyl polymer 1.0 Preservatives, fragrances, and colors a very small amount Distilled water Balance Sum 100

Manufacturing example 3: BB cream

Preparation examples of BB cream using the cosmetic composition of Example 2 are shown in Table 13 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Glycerin monostearate 1.5 Cetearyl alcohol 1.5 Stearic acid 1.0 Polysorbate 60 1.5 Sorbitan stearate 0.6 Isostearyl isostearate 5.0 Squalane 5.0 Mineral oil 35.0 Dimethicone 0.5 Hydroxyethyl cellulose 0.12 glycerin 6.0 Triethanolamine 0.7 Preservatives, fragrances, and colors a very small amount Distilled water Balance Sum 100

Manufacturing example 4: essence

Examples of the preparation of the essence using the cosmetic composition of Example 2 are shown in Table 14 below.

ingredient Content (% by weight) Composition of Example 2 1.5 glycerin 10.0 Betaine 5.0 PEG 1500 2.0 Allantoin 0.1 DL-panthenol 0.3 EDTA-2Na 0.02 Benzophenone-9 0.04 Hydroxyethyl cellulose 0.1 Sodium hyaluronate 8.0 Carboxyvinyl polymer 0.2 Triethanolamine 0.18 Octyldodecanol 0.3 Octyldodeces-16 0.4 ethanol 6.0 Preservatives, fragrances, and colors a very small amount Distilled water Balance Sum 100

Manufacturing example 5: mask For pack latex

An example of preparing an emulsion for a mask pack using the cosmetic composition of Example 2 is shown in Table 15 below.

ingredient Content (% by weight) Composition of Example 2 1.0 Polyvinyl alcohol 15.0 Cellulose gum 0.15 glycerin 3.0 PEG 1500 2.0 Cyclodextrin 0.15 DL-panthenol 0.4 Allantoin 0.1 Monoammonium glycyrrhizinate 0.3 Nicotinamide 0.5 ethanol 6.0 PEG 40 hydrogenated castor oil 0.3 Preservatives, fragrances, and colors a very small amount Distilled water Balance Sum 100

Claims (10)

Lactobacillus plantarum (Lactobacillus plantarum) culture extract, star anise extract, Virginian algae leaf extract, Indian goose berry extract, and a mixed extract of mulberry bark and sky tarry root,
The mixed extract of the mulberry bark and the sky tarri root is an extract obtained by fermenting a mixture of the mulberry bark and the sky tarri root in a weight ratio of 1: 0.2-0.8 with Lactobacillus plantarum. delete The cosmetic composition according to claim 1, wherein the Lactobacillus plantarum culture extract is an extract obtained by culturing Lactobacillus plantarum and crushing cells with a homogenizer. The cosmetic composition of claim 1, wherein the star anise extract is an extract obtained by fermenting star anise with Lactobacillus plantarum. delete delete The cosmetic composition according to claim 1, wherein the mixed extract of the mulberry bark and the sky tari root is mixed in an amount of 5 to 40 parts by weight based on 100 parts by weight of the Lactobacillus plantarum culture extract. The method of claim 1, wherein the cosmetic composition comprises 5 to 40 parts by weight of star anise extract, 1 to 30 parts by weight of virginia leaf extract, and 1 to 20 parts by weight of Indian goose berry extract based on 100 parts by weight of Lactobacillus plantarum culture extract. Cosmetic composition, characterized in that included. The method of claim 1, wherein the cosmetic composition is BB cream, skin lotion, skin softener, skin toner, astringent, lotion, milk lotion, moisture lotion, nutrition lotion, massage cream, nutrition cream, sunscreen cream, moisture cream, hand cream , Foundation, essence, nutritional essence, mask pack, soap, cleansing foam, cleansing lotion, cleansing cream, body lotion and body cleanser. The cosmetic composition according to claim 1, wherein the cosmetic composition has an effect of improving skin wrinkles, a whitening effect, and increasing a duration of moisture retention.