KR101212346B1 - Cosmetic compositions for preventing and curing acne - Google Patents

Abstract

The present invention relates to a cosmetic composition that is effective in preventing and treating acne and having excellent safety, and more particularly, inhibits 5-lipoxygenase activity and thus has an anti-inflammatory effect and is safe for skin and excellent in treating acne. It relates to a cosmetic composition containing an extract. The cosmetic composition of the present invention is obtained by adding and mixing an extract extracted with Cnidium officinale Makino and Gynostemma pentaphyllum (Thunberg) Makino as a solvent or a mixture of water or an organic solvent at a predetermined ratio to a conventional cosmetic It is characterized by.

Description

Cosmetic compositions for treating and preventing acne

The present invention relates to a cosmetic composition that is effective in preventing and treating acne and having excellent safety, and more particularly, to inhibit 5-lipoxygenase activity, which has an anti-inflammatory effect, is safe for skin, and has an excellent acne prevention and treatment effect. It relates to a composition.

Acne is a chronic inflammatory disease of the hair follicle sebaceous gland, which occurs mainly in puberty men and women and is characterized by the formation of cotton, papules, pustules, cysts or nodules. Acne usually occurs in puberty and declines in the mid-20s, but it also occurs in adults. Women develop acne faster than men, but appear more severe in men. It is reported that there are four kinds of factors that cause acne, excluding genetic and environmental factors. The first is the development of acne due to excessive sebum secretion in the sebaceous glands. Sebum formation begins when testosterone, a male hormone, is converted to dihydrotestosterone by a 5-alpha-reductase enzyme. In other words, the abnormal increase in male hormone or related mechanisms promotes sebaceous gland function, resulting in excess sebum. Second is scleroderma by hair follicle hyperkeratosis. The generated sebum should be discharged smoothly out of the skin, but due to the hyperkeratosis of the hair follicles, the pores are blocked and the smooth discharge of sebum is suppressed. Cotton cloth is divided into closed and open cotton cloth. The obstructive scrim is characterized by protruding sebum that is not secreted out of the sebaceous glands and sticking out like a millet. Open scalp refers to acne that looks like dots, with stagnant scalp clogging the pores in the form of lumps and oxidizing the tip to black. The third is the development of acne by overproliferation of acne bacteria (Propionibacterium acnes). Acne bacteria are present in the sebaceous glands as skin flora, but by increasing sebum secretion, acne bacteria also multiply, and triglyceride, a sebum component, is converted into free fatty acid to stimulate acne. Fourth is the formation of acne by inflammatory reactions. Inflammatory substances produced by acne bacteria cause inflammatory reactions such as red swollen papules and pustules, including pus (Korean Society for Skin Barriers, Vol. 4, No. 1: 62-70. 2000)).

The release and metabolism of arachidonic acid from the cell membrane produces pro-inflammatory metabolites by several different pathways. There are two important pathways, mediated by 5-lipoxygenase (5-LOX) and cyclooxygenase (COX). These pathways produce leukotrienes and prostaglandins, each of which play an important role in the initiation and progression of the inflammatory response, which promotes tissue infiltration of inflammatory cells and prolongs the inflammatory response. In particular, leukotrienes produced by 5-lipoxygenase (5-LOX) are known to be involved in skin diseases such as eczema, erythema, psoriasis, pruritus and acne ( Pharmacology , Mary J. Mycek).

In order to prevent and treat acne, sebum secretion control, hyperkeratosis control, acne bacteria hyperproliferation control, inflammatory response control, etc. should be considered. Conventionally, it regulates sebum secretion (Korean Patent No. 10-0368126, Korean Patent Publication No. 2001-0097012), and inhibits the growth of acne bacteria Propionibacterium acne (Korean Patent Publication No. 2002-0044851, 2003-0084484, 2003-0082676) Although studies have been reported, few studies have been conducted regarding inflammatory response mechanisms.

In the meantime, the salicylic acid and vitamin A derivative retinoic acid preparations are used to remove the dead skin cells and benzoyl peroxide to inhibit the growth of acne bacteria. Or it can cause skin scleroderma, benzoyl peroxide has irritation and severe itching and burning side effects. Topical coating antibiotics such as clindamycin, erythromycin, tetracycline, and sulfonamide are used for the treatment of inflammatory acne (Dermatology, Korean Dermatology Textbook Compilation Committee). The reaction may cause erythematous papules with pruritus (Korean Journal of Dermatology, 38 (1): 1-14 (2000)). As described above, in order to treat acne, most studies on sebaceous gland secretion, acne bacteria growth inhibition, cotton cloth dissolution, and the like, an anti-inflammatory study for treating acne, a chronic inflammatory disease of the sebaceous gland, is urgently needed.

In view of these problems, the present inventors have studied to develop cosmetics that are excellent in the prevention and treatment effect of acne and excellent in the safety even for long-term use, and have anti-inflammatory effects and are safe for skin. A cosmetic containing was completed.

The present invention for achieving the above object provides a cosmetic composition for treating and preventing acne containing an extra stone extract.

In another aspect, the present invention provides a cosmetic composition for treating and preventing acne containing a dodol extract and a cheongung extract.

Cosmetic composition for the treatment and prevention of acne of the present invention is a ratio of the extract extracted with Cnidium officinale Makino and Gynostemma pentaphyllum (Thunberg) Makino as a solvent or a mixture of water or an organic solvent, respectively, in a certain proportion to a conventional cosmetic It is characterized by the addition obtained by mixing.

According to another configuration of the present invention, the organic solvent is preferably one or more selected from acetone, ethyl acetate, diethyl ether, benzene, chloroform, hexane, lower alcohol having 1 to 4 carbon atoms in a predetermined ratio. May be used.

Furthermore, in the cosmetics containing the cheongoung and doldol extract according to the present invention, the formulation of the cosmetic product to which the cheongoung and doldol extract of the present invention is added is added to conventional cosmetic formulations such as flexible lotion (skin), lotion, essence, cream, etc. Can be prepared.

The present invention relates to a cosmetic composition having the effect of preventing and treating acne by containing the amounts of 0.001 to 10% by weight, respectively, or mixed with the cheongung and doldol extract based on the dry weight of the cosmetic.

Cnidium officinale Makino used in the present invention is a perennial herb that is commonly cultivated in fields in various parts of our country as it corresponds to the mountainous tree type and the mud. The height is 30-60cm and the leaves are regenerated, two-times. The flowers are white and the double acidic flowers are large, about 15 small mountain flowers, and 5-6 small guns and small guns. It is a plant without fruit (Korean Plant Book, Lee Young-no). As for the uterus, it has been reported as a composition component of a cosmetic for sensitive skin, and is known as an antiulcer therapeutic agent and a cosmetic composition for skin whitening.

Dol-Eup ( Gynostemma pentaphyllum (Thunberg) Makino) is a perennial vine plant of the dicotyledonous gourd, which is distributed at the edge of forests in southern Korea, Gyeongsangbuk-do, and Ulleungdo Island. Node with white hairs, tendrils on stems, leaves regenerated, palm-shaped double leaves, small leaves 3-7, membranous, narrow egg-shaped oval, narrow ovate, pointed end, multicellular white hairs on both sides It disappears, 4-8cm long, 2-3cm wide, with teeth on the edges. Flowers run from bisexual, yellow-green, axillary to conical or gunshot inflorescences, 8-15 cm long. Calyx branch is extremely small, corolla is 5 branch, branch length is 3mm, lanceolate, pointed end. Fruits are gourd, round, 6-8mm in diameter, black green, with one horizontal line in the upper half (Korean Plant Book, Lee Young-no). Regarding Dol et al, the preparation method of health drink tea (JP 1019840003341, EP 1019840007337), antioxidant effect (Zhong Yao Cai. 1999 Jun; 22 (6): 303-6, Nitric Oxide. 2003 Jun; 8 (4): 235-42 ), Studies on the Components Isolated from Stones (J Nat Prod. 1995 Apr; 58 (4): 512-9, J Nat Prod. 1996 Dec; 59 (12): 1143-5, Eur J Pharm Sci. 1999 Jul. ; 8 (3): 187-91).

Hereinafter, the present invention will be described in more detail with reference to the following experimental examples. However, these experimental examples are only for the purpose of understanding the present invention, and the scope of the present invention is not limited to the following examples and experimental examples.

Experimental Example 1. 5-lipoxygenase activity inhibition experiment

In a 15 ml tube, 1 ml of linolenic acid, 0.1 ml of sample, and 0.9 ml of lipoxygenase were constantly mixed with a mixer. After incubation at 25 ° C. for 10 minutes, 0.5 ml of 20% trichloroacetic acid and 1 ml of 0.6% Thiobarbituric acid are added. It boiled in boiling water for 20 minutes and then cooled in ice water for 3 minutes. 2 ml of n-butanol was added to the tube, mixed with a mixer for 20 seconds, and centrifuged at 3500 rpm for 5 minutes. The supernatant was measured for absorbance at 535 nm, indicating a percent inhibition of lipoxygenase. Inhibition rate was calculated according to the following equation.

Nodihydroguaiaretic acid (NDGA) was used as a positive control, and dolga was extracted with ethanol, and then a powdered product prepared by volatilizing the solvent under reduced pressure was tested in phosphate buffered saline (PBS). Diluted to concentration and used. Treatment was performed according to the experimental example to obtain a result as shown in Table 1.

5-lipoxygenase activity inhibitory effect density(%) % Inhibition
NDGA Celestial extract Dolan extract 0.01 47.5 38.6 29.8 0.1 86 81.2 66.4 One 94 82.7 72.1

As can be seen in Table 1, the extract of the uterus showed a corresponding effect with the control NDGA at 0.1% concentration, and the extradol extract showed an effective effect by inhibiting 5-lipoxygenase activity in a concentration-dependent manner.

Experimental Example 2. Anti-inflammatory experiment using mouse ears

In order to examine the anti-inflammatory effects on dodol extracts and uterine extracts, the mouse left ear was used as the control site and the right ear as the test site, and the ear was washed thoroughly with ethanol before applying the sample. The application was continued for a day. One hour after the last application, ethanol was applied to the left ear and 2 mg of arachidonic acid was applied to the right ear. Anti-inflammatory effect was determined as the degree of edema inhibition based on the arachidonic acid treatment group and the results are shown in Table 2. Inhibition rate was calculated according to the following formula.

A: Average thickness of control ears (thickness of edema after arachidonic acid treatment)

B: average thickness of test group ears (thickness of edema after sample treatment)

Anti-Inflammatory Effects of Dodol Extract and Cervix Extracts sample density(%) menstruum % Inhibition Stearyl Glycyrretinate 0.1 ethanol 4.5 Celestial extract 0.1 ethanol 7.8 Celestial extract 0.01 ethanol 4.3 Dolan extract 0.1 ethanol 6.3 Dolan extract 0.01 ethanol 5.1 Cheonggung and Dool Extract Mixed Samples (1: 1) 0.1 ethanol 9.7 Cheonggung and Dool Extract Mixed Samples (1: 1) 0.01 ethanol 6.5

As can be seen from the above results, the archery showed an inhibition rate of 0.1% to 7.8%, and the dodol showed an inhibition rate of 6.3% at 0.1% concentration. The effect was higher than the inhibition rate of 4.5% at 0.1% of stearyl glycretinate, which is known to have good anti-inflammatory effect. In particular, the sample containing the same amount of cheongung extract and dodol extract showed an inhibitory rate of inflammation from 0.1% to 9.7%.

Experimental Example 3. Acne Treatment Compatibility Test

The purpose of this study was to investigate the effect of acne treatment and skin irritation with cosmetics made by adding 0.1% of the samples prepared in the same amount to the common lotion and skin formulations. The subjects were women between the ages of 16 and 18 years old and consisted of students with multiple papules, inflammatory acne on the face. Each group consisted of 15 people and a blind test was performed during the evaluation. Subjects used skin and lotion cosmetics containing 0.1% extra stone extract twice a day, morning and evening, and after two months of application, subjects evaluated by subject questionnaire and visual evaluation by tester.

Table 3 shows the acne treatment effect on a five point scale, Table 4 shows the results of confirming whether the irritation to the skin of the cosmetics during the evaluation through a questionnaire.

Acne Treatment Lotion skin

Subject Subjective Assessment 5 2 One 4 11 11 3 3 2 2 - One One - - Average 4.2 3.8

Visual assessment of the examiner 5 One One 4 10 12 3 4 One 2 - One One - - Average 3.8 3.86

* 5 point scale

5: very good, 4: better, 3: not sure, 2: worse, 1: very bad.

Skin irritation pepper Number of people Lotion skin have - - none 15 15

As shown in Table 3, the lotions and skins containing 0.1% of the uterus and extra stone mixture extracts were 4.2 and 3.8 in the subject's subjective evaluation and 3.8 and 3.86 in the subject's visual evaluation, respectively.

In addition, as shown in Table 4, as a result of using a lotion and skin containing acne mixed extracts and cheongung for two months subjects with acne, it can be seen that there is no irritation to the skin is safe.

The cheongung and dodol extracts according to the composition of the present invention have 5-lipoxygenase activity inhibition and anti-inflammatory effects in animal experiments, and furthermore, they are effective in preventing and treating acne when these extracts are mixed. In addition, the skin is also safe.

Claims (4)

A cosmetic composition for preventing and improving acne, characterized in that it contains a dodol extract and a cheongung extract. delete delete The cosmetic composition for preventing and improving acne according to claim 1, wherein the cheongung extract and the extra stone extract are mixed 1: 1 to contain 0.001 to 10.0 wt% based on the total weight of the composition.