JPWO2008075718A1 - 蛍光発生分子 - Google Patents
蛍光発生分子 Download PDFInfo
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- JPWO2008075718A1 JPWO2008075718A1 JP2008550172A JP2008550172A JPWO2008075718A1 JP WO2008075718 A1 JPWO2008075718 A1 JP WO2008075718A1 JP 2008550172 A JP2008550172 A JP 2008550172A JP 2008550172 A JP2008550172 A JP 2008550172A JP WO2008075718 A1 JPWO2008075718 A1 JP WO2008075718A1
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Abstract
Description
本発明の標識試薬は、好ましくは、核酸の標識のために用いるものであり、好ましくは、還元剤と組み合わせて使用されるものである。
好ましくは、上記無蛍光性分子として、上記した本発明の化合物を用いる。
好ましくは、標的核酸配列はRNAである。
好ましくは、標的核酸配列の一塩基多型を検出する。
好ましくは、上記した本発明の化合物を用いて細胞内の生体分子を標識する。
好ましくは、上記した本発明の化合物を用いて細胞内の生体分子を標識し、該化合物に光を照射してアジド基をアミノ基に還元させることにより生成する蛍光をフローサイトメトリーで検出する。
本発明で開発した蛍光発生分子システムは、アジド基の還元をトリガーとして、無蛍光性分子の構造変化が起こり蛍光を発する蛍光off/on型センサーシステムとして一般化できる(図1)。本発明の実施例として、蛍光分子ローダミンを化学修飾し、アジド基を導入した分子を合成した(図2)。ローダミン110を、モノBOC化した。つづいて、亜硝酸イソアミルで処理することにより、アミノ基をジアゾ化し、ナトリウムアジドを加えることによりアジド体を得た。さらに、4M 塩酸-ジオキサン溶液で処理することにより、BOC基を除去した。最後に、アミノ基をブロモアセチル化することにより、目的物であるローダミンのアジド誘導体(図2の化合物4)を得た。
本発明において、アリール基としては、フェニル基、又はナフチル基を挙げることができる。
本発明において、酸素原子を含む基としては、−O−、又は−OCO−などを挙げることができる。
(1)Azido-Rh110-t-Boc (図2の化合物2) の合成
アルゴン雰囲気下、モノBOC化ローダミン(図2の化合物1)(48.0mg, 0.112mmol)をアセトニトリル(8mL)と塩化メチレン(4mL)に溶かした。さらに、0℃下、トリフルオロ酢酸(12.4μl, 0.167mmol)、亜硝酸イソアミル(17.8μl, 0.134mmol)を溶液中に加え攪拌した。0℃下、40分攪拌した後、ナトリウムアジド(15.0mg, 0.223mmol)を加えて室温下30分攪拌した。飽和重曹水を加えて反応停止後、酢酸エチルを加えた。有機層を、無水硫酸ナトリウムで乾燥した。減圧下溶媒を留去し、残渣をフラッシュシリカゲルカラムクロマトグラフィーで精製することにより、azido-Rh110-t-Boc (図2の化合部う2) (36.5mg, 80.0μmol, 71%)を黄色結晶として得た。
13C NMR ( 400MHz, CDCl3 ) δ 28.36, 81.25, 82.19, 105.97, 107.21, 112.87, 114.26, 114.74, 115.59, 123.70, 125.07, 126.22, 128.45, 129.43, 129.78, 135.04, 140.56, 142.37, 151.47, 152.08, 152.95, 169.13
QSTAR (Applied Biosystems/MDS SCIEX) (ESI): [MH+] C25H21N4O5: 457.1512, found: 457.1509
Azido-Rh110-t-Boc (図2の化合物2) (30.8mg, 67.5μmol)を4M 塩酸ジオキサン溶液(3mL)に溶解し、室温下2時間半攪拌した。溶媒を留去し、TLCプレートにより精製することによりAzido-Rh110 (図2の化合物3) (23.8mg, 66.8μmol, 99%)を淡桃色固体として得た。
13C NMR ( 400MHz, CDCl3 ) δ 82.82, 101.15, 106.95, 108.15, 111.67, 114.39, 115.82, 123.63, 124.80, 126.54, 128.93, 129.38, 129.48, 134.73, 142.03, 148.62, 151.97, 152.11, 152.73, 169.06
QSTAR (Applied Biosystems/MDS SCIEX) (ESI): [MH+] C20H13N4O3: 357.0988, found: 357.0988
Azido-Rh110 (図2の化合物3) (17.9mg, 50.2μmol)をクロロホルム(4mL)に溶解し、無水炭酸カリウム(138.2mg, 1.0mmol)を加えた。氷零下、ブロモアセチルブロマイド(43.7μl, 0.50mmol)をゆっくりと反応液に加え、反応液を2時間室温下攪拌した。その後、飽和重曹水を加えて反応を停止した。水層をクロロホルムで2回抽出し、有機層を無水硫酸ナトリウムで乾燥した。反応液を濃縮し、残渣をシリカゲルクロマトグラフィーを用いて精製し、目的物であるbromoacetylamido-Rh110-azido (図2の化合物4) (22.6mg, 47.4mmol, 94%)を淡黄色の固体として得た。
13C NMR ( 400MHz, CDCl3 ) δ 29.21, 81.89, 107.11, 107.82, 114.86, 115.15, 115.43, 123.57, 125.03, 125.87, 128.38, 129.26, 129.82, 135.12, 138.86, 142.46, 151.09, 151.77, 152.68, 163.60, 169.11
QSTAR (Applied Biosystems/MDS SCIEX) (ESI): [MH+] C22H14BrN4O4: 477.0198, found: 477.0196
実施例1で合成したアジド誘導体(図2の化合物4)は、無蛍光性であった。次に、この化合物(図2の化合物4)をメタノール溶液中、還元剤であるTCEP(リン化合物)(トリス[2−カルボキシエチル]ホスフィン)で処理したところ、アジド基はアミノ基に変換され、高い蛍光性を示した。処理前と比較し、約2000倍蛍光強度が増加していた(図3)。
全てのオリゴヌクレオチドは、0.2 μMスケールのカラムを用いて、一般的なホスホロアミダイト法に基づいて、DNA自動合成機(H-8-SE; Gene World)で合成した。塩基の脱保護およびCPG担体からの切り出しは、アンモニア水中で、55℃、4時間インキュベートすることにより行った。オリゴヌクレオチドの精製は、逆相カラム(MicroPure II; Biosearch Technologies)にて行い、UV absorbanceを測定することにより濃度を決定した。
Triphenylphosphine groupの付加は、 5' amino-modifiedオリゴと反応させることにより行った。 5' amino-modified オリゴの合成には、5' amino-modifier 5 (Glen Research)を用いた。反応は、8 mM のtriphenylphosphine NHS ester (in DMF)、50 mMのsodium tetraborate buffer、200 μMの5' amino-modifiedオリゴ溶液を含む混合液を、室温で3時間激しく撹拌することにより行った(反応液中のDMF濃度は46%)。 反応産物をエタノール沈殿で回収したのち、逆相HPLC (グラジエント条件: 0−50 % acetonitrile/50 mM triethylammonium acetate)にて精製を行った。また、ESI-TOF mass spectrometryにより目的の産物が得られていることを確認した。
5'-TGTGGGCAtriphenylphosphine-3': calculated mass, C104H126N33O51P9 2931.6; found 2932.6.
Bromoacetylamido-Rh110-azido (4)の付加は、 3' phosphorothioateオリゴと反応させることにより行った。 3' phosphorothioate オリゴの合成は、3'-phosphate CPGに始めのモノマーをカップリングしたのち、sulfrizing reagent (Glen research)でホスホロチオエート化することにより行った。反応は、3 mM のBromoacetylamido-Rh110-azido (in DMF)、80 mMのtriethylammonium bicarbonate buffer、300 μMの3' phosphorothioateオリゴ溶液を含む混合液を、室温で5時間激しく撹拌することにより行った(反応液中のDMF濃度は80%)。 反応産物をエタノール沈殿で回収したのち、逆相HPLC (グラジエント条件: 0−80 % acetonitrile/50 mM triethylammonium acetate)にて精製を行った。また、ESI-TOF mass spectrometryにより目的の産物が得られていることを確認した。
5'-GCCGGCGGRh110-azide-3': calculated mass, C104H126N33O51P9 2942.5; found 2942.5.
DNAテンプレート上での反応は、それぞれ500 nMのDNAテンプレート、5' Triphenylphosphine-linked プローブ、3' Bromoacetylamido-Rh110-azido -linkedプローブをLigation buffer中(70 mM Tris-base, 70 mM boric acid, 10 mM MgCl2 6H2O; pH 8.0)において、25℃で反応させることにより行った。用いた配列は以下の通りである。
5' Triphenylphosphine-linked プローブ:5'- TGT GGG CA -3'
5' Bromoacetylamido-Rh110-azido -linkedプローブ:5'- GCC GGC GG -3'
QSTAR (Applied Biosystems/MDS SCIEX) (ESI): [MH+] C29H14N6O5: 527.103, found: 527.117
実施例5で合成したビスアジド誘導体の蛍光特性を解析した。ビスアジド誘導体は、無蛍光性であった。次に、ビスアジド誘導体をメタノール溶液中、還元剤であるTCEP(リン化合物)で処理したところ、アジド基はアミノ基に変換され、高い蛍光性を示した。その蛍光波長は、664に最大強度を示し、赤色蛍光であった。また、処理前と比較し、約1500倍蛍光強度が増加した(図7)。
実施例1で合成したアジド誘導体(図2の化合物4)は、無蛍光性であった。次に、この化合物(図2の化合物4)の1μMメタノール溶液を、350nmの紫外光で約60秒間照射した。その後、490nmの励起光により、蛍光スペクトルを測定した(図8)。本化合物は、光照射前は、蛍光はないが、光照射後、490nmの励起波長で観察される蛍光を発する。
図9にテンプレートおよびプローブの配列を示す(Az:アジド基修飾した蛍光基,Red:還元剤)。テンプレートは,フルマッチのDNAおよび一塩基変異のDNA,さらにフルマッチのRNAを用意した。本プローブを用いて、実施例6と同様の方法で、ヒトras遺伝子配列の検出実験を行った結果、DNAをテンプレートとした場合と同様に,RNAをテンプレートとした場合も,標的配列が存在すると、蛍光シグナルが顕著に増大した(図10)。また,一塩基変異を識別することも可能であった(図11)。
E.coli K12 (NBRC 3301)を、オートクレーブ滅菌したLuria-Bertani Broth (NIHON SEIYAKU)の中、30℃で20時間振とう培養した。培養液を5,000rpmで5分間遠心分離し、上澄みを捨て、菌体をphosphate-buffered saline buffer (pH7.2)中で再分散した。分散させた液を4% paraformaldehhydeで固定した (Amann et al. (1990) J. Bacteriol. 172:762-770.)。
ラボテックチャンバー(Nunc)上で細胞を培養し,細胞をMg, CaフリーのPBSで2度洗浄した。洗浄した細胞に,600nMのプローブ(28S rRNAを標的としたプローブ)と10U/mlのStreptolysin O(SLO;Sigma-aldrich)を300μL各チャンバーに加え,室温で10minインキュベートした。このときSLOは,100mMのDTT中で2時間インキュベートし,十分に活性化したものを用いた。10分のインキュベート後,0.2g/LのCaCl2を含むMEM培地を各ウェルに300uL加えてSLOの不活性化した。さらに37℃で1hrインキュベートしたのち,PBSで細胞を1度洗浄した。検出した細胞は,蛍光顕微鏡(Zeiss)下で,ex. 470/40, em. 525/50のフィルターを用いて撮影した。結果を図14に示す。図14において、Aはrhodamine azideプローブ+phosphine プローブ(明視野)、Bはrhodamine azideプローブのみ(明視野)、Cはrhodamine azideプローブ+phosphine プローブ(蛍光)、Bはrhodamine azideプローブのみ(蛍光)を示す。フルマッチのプローブを導入後、約5分で蛍光シグナルが観察された(図14)。一方、ミスマッチプローブにおいては、全くシグナルは観察されなかった。
Claims (13)
- 請求項1又は2に記載の化合物を含む、生体関連物質を検出するための標識試薬。
- 核酸の標識のために用いる、請求項3に記載の試薬。
- 還元剤と組み合わせて使用される、請求項3又は4に記載の試薬。
- 標的核酸配列の一部の領域と相補的な核酸配列を有する第一の核酸プローブであって、ローダミン骨格、クマリン骨格、エチジウムブロミド由来骨格、アクリジン骨格又はダンシル骨格から選択される骨格を有し、かつ分子内にアジド基を有する無蛍光性分子で標識した上記の第一の核酸プローブと、標的核酸配列の別の一部の領域と相補的な核酸配列を有する第二の核酸プローブであって、還元作用を有する分子で標識した上記の第二の核酸プローブとを、標的核酸配列にハイブリダイズさせる工程、及び第一の核酸プローブ中の無蛍光性分子のアジド基がアミノ基に還元させることにより生成する蛍光を検出する工程を含む、標的核酸配列の検出方法。
- 上記無蛍光性分子として、ローダミン骨格を有し、かつ分子内にアジド基を有する無蛍光性分子を使用する、請求項6に記載の方法。
- 上記無蛍光性分子として、請求項1又は2に記載の化合物を用いる、請求項6又は7に記載の方法。
- 標的核酸配列がRNAである、請求項6から8の何れかに記載の方法。
- 標的核酸配列の一塩基多型を検出する、請求項6から8の何れかに記載の方法。
- 請求項1又は2に記載の化合物を用いて生体分子を標識し、該化合物に光を照射してアジド基をアミノ基に還元させることにより生成する蛍光を検出することを含む、生体分子の分析方法。
- 請求項1又は2に記載の化合物を用いて細胞内の生体分子を標識する、請求項11に記載の生体分子の分析方法。
- 請求項1又は2に記載の化合物を用いて細胞内の生体分子を標識し、該化合物に光を照射してアジド基をアミノ基に還元させることにより生成する蛍光をフローサイトメトリーで検出する、請求項11又は12に記載の生体分子の分析方法。
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US9212189B2 (en) * | 2011-06-10 | 2015-12-15 | The Regents Of The University Of California | Fluorescent probes for reactive sulfur species |
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US6448407B1 (en) | 2000-11-01 | 2002-09-10 | Pe Corporation (Ny) | Atropisomers of asymmetric xanthene fluorescent dyes and methods of DNA sequencing and fragment analysis |
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