JP3105050B2 - Aseptic implanter - Google Patents

Description

DETAILED DESCRIPTION OF THE INVENTION

[0001]

BACKGROUND OF THE INVENTION 1. Field of the Invention The present invention relates to a method for aseptically implanting tissue organs such as plants and animals that have been treated aseptically into a culture tank such as a tank, or to the atmosphere of microorganisms and viruses. TECHNICAL FIELD The present invention relates to a sterile implanting device used when performing an operation of transferring a substance while preventing leakage of water.

[0002]

2. Description of the Related Art In the handling of substances such as cultures of microorganisms, plant and animal tissues and organs, resins, immobilized carriers, solid nutrients, high-viscosity media, etc., from the apparatus containing these substances to other culture vessels In many cases, it is necessary to perform an operation of transferring the substance while preventing contamination or leakage.

[0003] At this time, the operation such as transportation is often accompanied, and it is necessary to aseptically transport and aseptically transfer to a counterpart container such as a culture tank.

[0004] For example, when assembling the buds of plant organs aseptically and transferring them to one culture tank, the buds are once collected in a transfer container as an aseptic implanter in a clean room, and the buds are transferred to the culture tank. After aseptically connected, the shoots are put into a culture tank.

[0005]

In the above-mentioned charging operation, buds are often clogged at the entrance and exit of the transfer container, and aseptic injection is often impossible or difficult.

In the case where the transfer becomes difficult due to the attachment of a solid material that requires aseptic handling, a medium containing the solid material, a highly viscous substance, etc., various contrivances have conventionally been made. No solution has been obtained.

SUMMARY OF THE INVENTION The present invention has been made to solve the above-mentioned problems, and an object of the present invention is to perform a transplanting operation without a substance to be transplanted being clogged or adhered to an entrance / exit portion during a transplanting operation. An object of the present invention is to provide an aseptic implantation device which can be aseptically performed.

[0008]

According to the present invention, there is provided, in accordance with the present invention, an aseptic implanting device having a container body having an access port through which a substance to be implanted is inserted and removed. And a sterile implanting device, wherein at least a part of the container body is formed of a bag-shaped container portion made of a flexible material, and a scraper is provided in the container body.

[0009]

In the aseptic implanting device having the above-mentioned structure, when the substance to be implanted is clogged in the entrance when the substance to be implanted is taken out of the container body, the bag-like container is placed outside the bag-like container. The operation of forcibly pushing out the clogging of the entrance / exit portion or scraping off the substance adhering to the inner wall of the container body is performed aseptically by grasping the scraping tool by hand or machine.

Hereinafter, the present invention will be described in detail with reference to the drawings.

FIG. 1 shows a sterile implanting device according to the present invention. The device comprises a container main body O containing a tissue organ or the like (hereinafter referred to as a sterile additive) as a substance to be implanted.
And a scraping tool B put in the container body O,
And a lid D for closing the mouth of the container body O.

Here, the aseptic additive is a solid substance or a highly viscous substance, and is a living thing or other solid substance or an amorphous substance.

The container body O includes a bag-shaped container portion A and an entrance / exit portion C connected to an opening of the bag-shaped container portion A.

The material of the bag-shaped container portion A is a heat-resistant and pressure-resistant flexible material such as transparent or translucent silicone rubber or a bellows-shaped container or a shape which does not cause extreme deformation or tearing when sterilized by an autoclave. It is made of a memory material or the like. The material is selected according to the adhesion of the object.

When the bag-like container portion A is provided with a bone-like support mounting ring 4 for holding the shape on the side surface of the bag portion 3 in order to hold and use the bag-like container portion A in an expanded state as necessary. There is also.

On the bottom of the bag-shaped container portion A, a liquid reservoir 1 for storing a liquid is provided via a liquid reservoir inlet 2 when a culture solution or the like put together with a sterile additive is mixed. .

The entrance C is made of a material such as a metal such as stainless steel or a heat-resistant plastic, and is connected to the opening 6 of the bag-shaped container A and to the mouth of the container main body O integrally with the connection 6. It comprises a cone portion 7 extending so as to narrow in a conical shape toward the end and a connecting pipe 7a further extending from the cone portion 7.

The connection between the connecting portion 6 of the entrance / exit portion C and the bag-shaped container portion A is attached using a flexible adhesive after curing, and is further fixed with a band or the like.

A support ear 9 for supporting the container main body O on the floor of a clean bench (not shown) is provided on a side wall of the connection pipe 7a. Further, a detachable lid opening / closing handle 10 for holding the container main body O when the lid D attached to the container main body O is rotated is provided on the side wall.

The mouth of the connection pipe 7a forms the mouth of the container main body O, and also forms the connection port 11 connected to the attachment port 17 such as a culture tank and the lid D.

The connection pipe 7a may be closed by forming an easily rupturable inner lid in the connection pipe 7a.

The connection port 11 has a cam portion 12 composed of a cam, a screw, a clamp, and the like so that the connection port 11 can be attached to the lid D and the attachment port 17 in a completely sealed state.

The lid D is made of a material such as a metal such as stainless steel or a heat-resistant plastic, and has a connection port 1 of the connection pipe 7a.
1, a lid 13 attached in a sealed state, a pressure equalizing nozzle 14 formed integrally with the lid 13, and a cap 16 for closing the opening of the pressure equalizing nozzle 14. The cap 16 may be a valve or the like.

On the side wall of the pressure equalizing nozzle 14, a container body O
Opening / closing handle 1 for rotating lid 13 with respect to
5 are provided.

Reference symbol E denotes a connection portion of a culture tank or the like to which the connection port 11 of the container body O is connected, and has a mounting port 17 made of a material such as metal or heat-resistant plastic.

Next, regarding the scraping tool B,
The scraping tool B includes a bar-shaped portion 5 and a disk-shaped disk 8 attached to the tip of the bar-shaped portion 5.

The length of the rod portion 5 is set longer than the axial length of the connection pipe 7a.

The scraping tool B can be in the form of a rod-shaped spoon, propeller, ring or the like according to the properties of the aseptic additive.

The operation procedure, operation and effect of the aseptic implanter of the present invention will be described below.

First, the aseptic implanter is sterilized (1). Then, the apparatus is supported (2), and a sterile additive is put into the apparatus (3) to separate adhering water (4). Then, the apparatus is transported to a culture tank or the like (5), the apparatus is connected to the culture tank or the like (6), and a sterile additive is introduced (7). After the introduction, the device is removed from the culture tank or the like (8).

Each of the above-mentioned operations will be described in detail below. (1) Sterilization of aseptic implanter The aseptic implanter is sterilized by an autoclave or the like.

At the time of sterilization, the pressure equalizing nozzle 14 and the cap 1
6 is loosened to allow ventilation, covered with breathable cotton and protected with aluminum foil. By doing so, the difference between the pressure inside the apparatus and the internal pressure of the autoclave becomes almost the same. Also,
The pressure equalizing nozzle 14 maintains the sterility of the air suction during the cooling of the autoclave.

As the material of the bag-shaped container portion A, a heat-resistant and pressure-resistant flexible material such as silicon rubber, a bellows-structured container, a shape memory material, or the like is used, so that no extreme deformation or breakage is caused by sterilization by an autoclave. . After sterilization, the cap 16 is sufficiently tightened by hand from the outside of the aluminum foil. (2) Support of aseptic implanting device The floor of a clean bench (not shown) is perforated so that a seal can be made, and the aseptic implanting device is attached to this hole. The mounting state is such that the supporting ears 9 are supported on the floor of the clean bench, so that the connection port 1 of the entrance / exit portion C of the aseptic implanting device is provided.
1 comes out on the floor of the clean bench, and the bag-shaped container part A is located under the floor.

Instead of supporting the apparatus with the holes of the clean bench and the supporting ears 9, a support may be used.

The support is manufactured for various situations.

When the aseptic implanter itself is used as a culture container, the bag-like container portion A is inflated and used by supporting the bone-like support mounting ring 4 in a stretched state. . (3) Operation of putting aseptic additive into the aseptic implanting device Remove the lid D fitted to the connection port 11 of the entrance C directed upward from the cam 12.

Then, a sterile additive is introduced through the connection port 11.

After the operation of putting the aseptic additive is completed, the lid 13 is closed by the operation in the flame, and the closing of the cap 16 is confirmed.

Since the bag-like container is made of a transparent or translucent material, the amount of the sterile additive can be determined. Since the bag-shaped container portion A is made of a flexible material, the volume of the bag-shaped container portion A itself can be reduced during autoclave sterilization, so that the storage volume in the bag-shaped container portion A is substantially equal to the amount of the aseptic additive. It can be set arbitrarily. (4) Separation of attached water A large amount of liquid such as a culture solution together with aseptic additives
This liquid mixes in the inner lower part, and this liquid drains when the aseptic implanter is attached to a culture tank or the like and impairs the sterility. Therefore, the liquid accumulates in the liquid reservoir 1 provided in the lower part of the bag-shaped container part A. After that, the liquid inlet 2 is closed with a pinch cock (not shown).

Instead of providing the liquid reservoir 1, a nozzle may be provided at the lower portion of the bag-shaped container portion A to drain the liquid therefrom. (5) Transportation of the aseptic implanting device In particular, the bag-shaped container portion A is placed in a basket or the like so as not to be damaged and transported to the place where the culture tank is installed. (6) Connection of aseptic implanter to culture tank, etc. Connect aseptically to culture tank, etc. using flame.

For the connection, the entrance C is turned downward, the lid D is removed in a flame, and the connection port 11 of the entrance C and the attachment port 17 of the culture tank are connected.

The attachment port 17 of the culture tank and the connection port 11 of the entrance / exit C of the aseptic implanter are provided with the cam portion 12, so that they can be aseptically sealed and fast and firmly fixed. Facilitates the operation of adding the next additive. This connection fixes the aseptic implanter and facilitates the operation of aseptic additive loading.

In order to prevent burning of the bag-shaped container portion A due to the flame,
A flameproof sheet (not shown) is used. For the flame, use a ring burner and attach a plate to block the flame.

The bag-shaped container portion A used for the connection work
It is preferable to attach a rod for suspending the aseptic implanter from above to the bone part for maintaining the shape of the flameproof sheet for preventing burnout. (7) Injection of sterile additive After connecting the aseptic implanter to the culture tank or the like, the flameproof sheet or the like is removed, and the sterile additive is scraped out of the bag-like container portion A together with the bag-like container portion A from the outside. It is grasped and operated by hand or machine and put into a culture tank or the like from an aseptic implanter.

Even in the case of a flowable sterile additive, the connecting pipe 7
By providing the inner lid inside a, dripping does not occur at the time of connection. Then, the aseptic additive is allowed to pass through by opening the inner lid of the connection pipe 7a using the scraper B or by breaking the inner lid with the scraper B.

In this operation, the mounting wheel 4 of the bag-shaped container portion A is not hung. This is because it hinders the operation of the scraper B. (8) Removal of aseptic implanting device After completion of implanting, aseptically remove the aseptic implanting device from the culture tank in a flame or the like as in the case of connection, and attach a lid for the culture tank to the attachment port 17 of the culture tank.

As described in the section (2), the aseptic implanter itself may be used as a culture tank.

When the present apparatus is to be made large, it is fixedly attached to a culture tank, and sterilization is performed by direct steam blowing into an aseptic inoculating apparatus.

The operation of putting the aseptic additive into the aseptic implanter is performed by, for example, setting a clean bench on the aseptic implanter as described in the section (2).

The scraping tool B and the container main body are only required if the clogging of the connecting portion 6, the cone portion 7 and the connecting pipe 7a of the entrance and exit can be forcibly pushed out, and the substance adhering to the inner wall of the container main body can be scraped off. The configuration, shape, material, and dimensions of O and the like are not limited to the present embodiment.

The present embodiment will be described below.

[0052]

【Example】

Example 1 A 10-liter container body O was charged with 3 kg of a plant body (the foliage of the edible soybean) as a sterile additive, and the scraping tool B described above (bar-shaped part: about 30 cm, disk: about 2 cm in diameter) was added. It was implanted in a 200-liter culture tank by the method used. After planting, the plants were aseptically cultured for 60 days to obtain 30 kg of rooted plants.

Example 2 2 kg of belladonna adventitious roots were placed in a container body O having a capacity of 5 liters, and 2 was prepared by using the scraping tool B described above.
It was implanted in a 00 liter culture tank. 3 after implantation
After culturing aseptically for 0 days, an adventitious root of 20 kg was obtained.

[0054]

As described above, in the present invention,
If the substance to be implanted comes out of the container body and the substance gets clogged in the doorway, grasp the scraper together with the bag-like container from the outside of the bag-like container by hand or machine, and then clog the doorway. Can be aseptically performed, for example, by forcibly extruding the material or scraping off the substance adhering to the inner wall of the container body.

[Brief description of the drawings]

FIG. 1 is a schematic configuration diagram of a sterile implantation device according to an embodiment of the present invention.

[Explanation of symbols]

 O Container body A Bag-shaped container B Scooping tool C Doorway D Lid E Incubation part for culture tank 1 Liquid reservoir 2 Liquid reservoir inlet 3 Bag 4 Bone-shaped support mounting ring 5 Rod-shaped part 6 Connection of entrance and exit Part 7 Cone part 7a Connection pipe 8 Disc-shaped disk of scraping tool 9 Support ear 10 Handle for opening / closing lid of entrance / exit part 11 Connection port of entrance / exit part 12 Cam part 13 Lid 14 Equalizing nozzle 15 Handle for lid opening / closing of lid 16 Cap 17 Mounting port for culture tank

──────────────────────────────────────────────────続 き Continuing on the front page (72) Inventor Tomojun Ujuku 15-33, Tojiraonakadai, Tsurumi-ku, Yokohama-shi, Kanagawa Examiner Eriko Suzuki (58) Field surveyed (Int.Cl. ⁷ , DB name) C12M 1/00-1/28

Claims (1)

(57) [Claims] 1. A sterile implantation device having a container body having an entrance through which a substance to be implanted is put in and out, wherein at least a part of the container body is made of a flexible material. An aseptic implanter comprising a scraper within the container body.