JP2018123078A - Oral composition for oral care - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide an oral composition that exhibits an excellent action to prevent and/or improve periodontitis.SOLUTION: An oral composition for oral care contains pine bark extract, and at least one plant material selected from Coffea arabica, Tilia miqueliana, Pueraria hirsuta, barley young leaves and sweet potato young leaves.SELECTED DRAWING: Figure 1

Description

  The present invention relates to an oral composition having an effect of preventing and improving periodontal disease.

  Periodontal disease is an inflammatory disease caused by bacterial infection. Bacteria in plaque (plaque) cause inflammation in the gums, and if left untreated, there is a risk of losing teeth. In addition, if this periodontal disease progresses, it is said that periodontal disease bacteria may be carried to systemic organs through capillaries in the mouth and cause systemic diseases such as diabetes, pneumonia, arteriosclerosis, and myocardial infarction. .

  As an oral composition for preventing and improving such periodontal diseases, for example, it has been reported that a composition containing a pine bark extract is effective (see Patent Documents 1 and 2).

  Although this pine bark extract has an effect of preventing and improving periodontal disease, further improvement of the effect is required.

WO2005 / 088784 JP 2001-511153 A

  This invention is made | formed in view of this situation, and the subject of this invention is providing the oral composition which has the outstanding prevention and / or improvement effect with respect to periodontal disease.

  The inventors of the present invention have been engaged in research on oral care, and in particular, as a result of intensive studies on foods and pharmaceuticals that more effectively prevent and / or improve periodontal disease, as a result, periodontal disease in which a specific plant material is a pine bark extract. As a result, the present inventors have found that the preventive / improving effect can be enhanced.

That is, the present invention is as follows.
[1] An oral composition for oral care, comprising a pine bark extract and at least one plant material selected from coffee tree, linden tree, kuzuka, barley young leaf and sweet potato young leaf.
[2] An oral care oral composition comprising a pine bark extract as an active ingredient, further comprising at least one plant material selected from coffee tree, linden tree, kuzuka, barley young leaf, and sweet potato young leaf Oral care oral composition.
[3] An oral composition comprising a pine bark extract and at least one plant material selected from coffee tree and linden tree.

  The oral composition of the present invention has an excellent preventive and / or ameliorating effect on periodontal disease.

It is a figure which shows the growth inhibitory effect with respect to Porphyromonas gingivalis of the composition (pine bark extract + coffee tree) of this invention. It is a figure which shows the growth inhibitory effect with respect to Porphyromonas gingivalis of the composition (pine bark extract + linden tree) of this invention. It is a figure which shows the growth inhibitory effect with respect to Porphyromonas gingivalis of the composition (pine bark extract + Kuzuka) of this invention. It is a figure which shows the growth inhibitory effect with respect to Porphyromonas gingivalis of the composition (pine bark extract + barley young leaf) of this invention. It is a figure which shows the growth inhibitory effect with respect to Porphyromonas gingivalis of the composition (pine bark extract + sweet potato young leaf) of this invention.

  The oral composition of the present invention comprises a pine bark extract and at least one plant material (sometimes referred to as an auxiliary material) selected from coffee tree, linden tree, kuzuka, barley young leaf and sweet potato young leaf. To do. An auxiliary material may be used individually by 1 type (for example, only coffee tree), and may be used in combination of 2 or more types. When two or more sub-materials are used in combination, it is preferable to combine sub-materials having a high effect of enhancing the effect of the pine bark extract.

  Since the combination of pine bark extract and plant material effectively inhibits the growth of Porphyromonas gingivalis, the causative agent of periodontal disease, the oral composition of the present invention effectively prevents and / or prevents periodontal disease. Can be improved. In addition, the oral composition of the present invention can suppress malodor produced when Porphyromonas gingivalis metabolizes proteins and amino acids, and can prevent and / or improve bad breath peculiar to periodontal disease.

[Pine bark extract]
The pine bark extract in the oral composition of the present invention contains proanthocyanidins as one of main components. Proanthocyanidins are a group of compounds composed of a condensation polymer having a degree of polymerization of 2 or more having flavan-3-ol and / or flavan-3,4-diol as a structural unit.

  Examples of the raw material of the pine bark extract include French maritime pine (Pinus Martina), larch, Japanese black pine, Japanese red pine, Japanese pine, Japanese pine, Korean pine, Japanese pine, Ryukyu pine, Utsukushima, Japanese pine, and white pine. Of these, French coastal pine is preferred. The obtained extract may be concentrated or dried as necessary to form a liquid, a paste, or a powder. Moreover, a commercial item can be used for a pine bark extract, for example, the pine bark extract (Flavandenol (trademark)) by Toyo Shinyaku Co., Ltd. can be used.

  Moreover, it is preferable that the pine bark extract in the oral composition of this invention contains the condensation polymer with a polymerization degree of 2 or more as proanthocyanidins. In particular, proanthocyanidins containing a large amount of a condensation polymer having a low degree of polymerization are preferred. Examples of the condensation polymer having a low degree of polymerization include a condensation polymer having a degree of polymerization of 2 to 30 (2 to 30 mer), and a condensation polymer having a degree of polymerization of 2 to 10 (2 to 10 mer). Preferably, a condensation polymer having a degree of polymerization of 2 to 4 (2 to 4 mer) is more preferable. In the present specification, a polymer having a polymerization degree of 2 to 4 is referred to as OPC (oligomeric proanthocyanidin). The pine bark extract in the oral composition of the present invention preferably contains 20% by mass or more of OPC, more preferably 30% by mass or more, and still more preferably 40% by mass or more.

  The pine bark extract is obtained by extracting pine bark with water or an organic solvent. When water is used, warm water or hot water is preferably used. Organic solvents used for extraction include foods or drugs such as methanol, ethanol, 1-propanol, 2-propanol, 1-butanol, 2-butanol, butane, acetone, hexane, cyclohexane, propylene glycol, hydrous ethanol, hydrous propylene glycol, etc. An organic solvent acceptable for the production of is preferably used. These water and organic solvents may be used alone or in combination of two or more solvents. In particular, water-soluble solvents such as water, ethanol, hydrous ethanol and the like are preferably used.

[Plant material (sub-material)]
In addition to the plant material itself, the plant material (sub-material) in the present invention may be a processed material such as a pulverized product, juice or extract. Examples of the pulverized product include powder and granules. The juice or extract may be liquid, but can also be used as a paste or dry powder. An extract can be obtained by extracting using a suitable solvent, and water-soluble solvents, such as water, ethanol, hydrous ethanol, can be used as a solvent, for example.
Hereinafter, each plant material will be described.

(Coffee tree)
The “coffee tree” used in the present invention is a plant belonging to the family Rubiaceae and Coffee genus (Coffea), for example, Arabic coffee tree (Coffee arabica), Robusta coffee tree (Coffee canephora Pierr.), Coffee robusta, Liberia coffee tree. (Coffee liberica) or the like can be used. As a site | part used as a plant raw material of this invention, a seed (coffee bean) is preferable, and what roasted may be used, but it is more preferable to use the raw seed which is not roasted.

(linden)
The linden tree is a plant of the Tiliaceae family, and the linden tree used in the present invention includes, for example, Tuna platyphyllos Scop., Tilia cordata Mill., And Tilia europaea L. Examples include plants of the family Lindenaceae and the genus Tilia. As a part used as a plant material of the present invention, a flower is preferred.

(Katsuhana)
As a kuzu flower, the flower of the plant which belongs to the legume genus Kudu is used. Kuzu is a perennial plant of the leguminous genus Kudzu. As Kuzuhana, what was collected in any process from the bud to the fully-opened flower may be used, and what was collected in each process can also be mixed and used. The type of kuzu is not particularly limited, and examples thereof include Pueraria thomsonii, Pueraria lobata, Pueraria thunbergiana, and the like. Pueraria thomsonii Is preferred.

(Barley young leaves)
Barley (Hordeum vulgare L.) is native to Central Asia and is an annual or perennial herb belonging to the family Gramineae, and is broadly classified into Nijo barley and Rokujo barley depending on the ear shape. The barley young leaves used as the plant material of the present invention are not particularly limited as long as they are usually available, and any barley young leaves such as Nijo barley and Rojo barley may be used. Any kind of barley young leaves may be used. The barley young leaf should just contain the barley young leaf, and other parts, such as a stem, may be contained.

(Gansu young leaves)
Sweet potato is a plant belonging to the convolvulaceae family and is generally called sweet potato. The sweet potato varieties are not particularly limited, and examples thereof include varieties such as Suiou, Joy White, Koganesengan, Shiroyutaka, Satsumasuchi, Ayamurasaki and the like. Of these, it is preferable to use a high polyphenol content. The sweet potato young leaf used as the plant material of the present invention only needs to contain sweet potato young leaves, and may contain other parts such as stems. In particular, the tip portion of the foliage (young stem leaf) is preferable, and the young stem leaf in a state of maintaining a yellowish green color is more preferable.

[Oral composition of the present invention]
The oral composition of the present invention includes, for example, pharmaceuticals (including quasi-drugs), functional foods whose indications of efficacy have been approved by predetermined organizations such as foods for specified health use, nutritional functional foods, functional labeling foods, etc. Can be used as so-called health foods, general foods, food additives, feeds and the like.

  The oral composition of the present invention can be used for oral care, particularly for prevention and / or improvement of periodontal disease. Such oral composition for oral care and oral composition for preventing and / or improving periodontal disease contains a pine resin extract and a plant material, and is used for oral care and preventing and / or improving periodontal disease. However, the product is not particularly limited as long as it can be distinguished from other products. For example, the indication of the effect of oral care on the body, packaging, instructions, or promotional material of the product according to the present invention and the effect of preventing and / or improving periodontal disease Included in the scope of rights.

  In addition, the oral composition for oral care and the oral composition for periodontal disease prevention and / or amelioration according to the present invention include the combination component (pine bark extract + sub-material) in the present invention in the product packaging, etc. It is not restricted to what is displayed as an active ingredient of care and periodontal disease prevention and / or improvement. For example, the active ingredient may not be specified. Moreover, what displayed only the pine bark extract as an active ingredient may be used. That is, as one form of the oral care oral composition or the periodontal disease prevention and / or improvement oral composition of the present invention, a pine bark extract is used as an active ingredient, and further, coffee tree, linden tree, kuzuhana, barley young leaf And a form containing at least one plant material selected from young sweet potato leaves.

  Specifically, oral compositions for oral care and oral compositions for preventing and / or improving periodontal disease of the present invention include pharmaceuticals (including quasi drugs) and so-called health foods. In so-called foods, for example, foods that have an effect of preventing and / or improving periodontal disease such as “maintain gum health”, “suppress bleeding from gums”, “shallow periodontal pocket” It can be illustrated.

  Examples of the oral composition of the present invention include tablets, capsules, powders, granules, liquids, granules, rods, plates, blocks, solids, rounds, pastes, creams, caplets , Gel, chewable, stick, and the like.

  The oral composition of the present invention is preferably in the form of a tablet, capsule, powder, granule, or liquid that is easy to ingest, and in particular, a beverage that stays in the oral cavity for a certain period of time, a chewable tablet, sputum, chewing gum and the like. Specifically, it can be dissolved in the oral cavity, in bottled beverages filled in PET bottles, cans, bottles, etc., or for dissolving and drinking in water (hot water), milk, fruit juice, green juice, etc. Examples include instant drinks, candy, chewing gum and the like. These are preferable in that they can be easily taken after meals and can improve palatability.

  The content of the pine bark extract and the plant material (component of the present invention) in the oral composition of the present invention may be appropriately contained within the range where the effect is exerted.

  In general, when the oral composition of the present invention is a pharmaceutical product or a supplement, the component of the present invention is preferably contained in an amount of 0.1 to 100% by mass in terms of dry mass, and 1 to 85% by mass. % Is more preferable, and 5-70 mass% is further more preferable.

  In order to exhibit the effect of the present invention more effectively, it is preferable that the component of the present invention is contained in 80% or more of the whole oral composition of the present invention in terms of dry mass, and contained in 90% or more. Is more preferably 95% or more, and particularly preferably 100%.

  The blending mass ratio between the pine bark extract and the plant material in the oral composition of the present invention is preferably in the range of 1: 0.001 to 1: 1000 in terms of dry mass, and is 1: 0.01. More preferably, it is in the range of ˜1: 500, and more preferably in the range of 1: 0.1 to 1: 200.

  The intake of the oral composition of the present invention is not particularly limited, but from the viewpoint of exerting the effects of the present invention more remarkably, the pine bark extract intake is 0.5 mg / day or more per day for an adult. Ingestion is preferable, more preferably 1 mg / day or more, and even more preferably 1.5 mg / day or more. The upper limit is, for example, 500 mg / day, preferably 400 mg / day. The oral composition of the present invention is not particularly limited as long as it requires oral care, but about 80% of adults in their 30s and 40s who are said to have periodontal disease, and elderly people with weak gums Is preferably taken.

  The oral composition of the present invention can be stored in one container or divided into a plurality of containers such as 2 to 3 so that the daily intake becomes the above-mentioned intake.

  The oral composition of this invention can be manufactured by a well-known method, adding components other than the component of this invention accept | permitted as oral use as needed. Examples of components other than the component of the present invention include water-soluble vitamins (vitamins B1, B2, B3, B5, B6, B12, B13, B15, B17, biotin, choline, folic acid, inositol, PABA, vitamin C, vitamin P ), Vitamins such as oil-soluble vitamins (vitamins A, D, E, K); minerals such as calcium, magnesium, phosphorus and iron; sulfur-containing compounds contained in taurine, garlic, etc .; flavanoids such as hesperidin and quercetin; Flavonoids; Proteins such as collagen; Peptides; Amino acids; Animal fats and oils; Vegetable fats and oils;

  Hereinafter, the present invention will be described based on examples.

[Example 1]
(Test method)
Modified GAM bouillon (Nissui) in which Porphyromonas gingivalis (hereinafter referred to as P. gingivalis) is anaerobic using aneropack kenki (Mitsubishi Chemical Co., Ltd.) and square jar (Mitsubishi Chemical Co., Ltd.) and autoclaved. (Manufactured by Pharmaceutical Co., Ltd.).
P. gingivalis was diluted with Dulbecco's phosphate buffered saline (manufactured by Nacalai Tesque Co., Ltd.) to prepare a turbidity of 0.5 McFarlamd. McFarland Standard (manufactured by Sysmex Biomelieu Co., Ltd.) was used as an index of turbidity.

  Modified GAM agar medium (manufactured by Nissui Pharmaceutical Co., Ltd.) was autoclaved and then poured into a sterile petri dish to prepare a plate medium. P. gingivalis prepared in the above 0.5 McFarland was uniformly applied to the plate medium.

  A test substance (pine bark extract and / or secondary material) was prepared to a predetermined concentration using PBS or DMSO. 15 μL of this test substance was dropped on a 6 mm filter paper for antibiotic testing (manufactured by GE Healthcare) and attached to a petri dish coated with the P. gingivalis.

  The cells were cultured for 2 to 3 days in an anaerobic environment at 37 ° C. using an anero-pack kenki, a square jar, and a thermostat.

  The diameter of the inhibition circle of each test substance formed in the petri dish was measured, and the diameter (6 mm) of the antibiotic test filter paper was divided. The growth inhibitory action of each test substance was corrected by the value obtained by dividing 6 mm from the diameter of the inhibition circle of isodine provided as a positive control on each plate (diameter of test substance inhibition circle−6 mm (mm) / diameter of isodine inhibition circle− 6 mm (mm)). The growth inhibitory action of each test substance was calculated as a ratio to the pine bark extract alone (50 mg / mL), and the growth inhibitory action of each test substance on P. gingivalis was evaluated.

In addition, as a test substance, the following commercial item or preparation was used.
[Pine bark extract]
A pine bark extract (Toyo Shinyakusha; Flavangenol (registered trademark)), which is a French coast pine bark extract, was used.

[Sub-material]
Coffee coffee (Coffee robusta) used the extract powder of commercially available coffee seeds (raw coffee beans).
For the linden tree (Tilia cordata Mill.), A commercially available flower extract powder was used.
For Kuzuka (Pueraria thomsonii), a hot water extracted flower extract powder (Toyo Shinyaku Co., Ltd.) was used.
The barley young leaves used an extract powder obtained by extracting pulverized powder (Toyo Shinyaku Co., Ltd.) with hydrous ethanol.
The sweet potato young leaves used the extract powder obtained by extracting the pulverized powder (Toyo Shinyaku Co., Ltd.) with hydrous ethanol.

  The evaluation result of the growth inhibitory action with respect to P. gingivalis of each test substance is shown in FIGS. FVG in the figure means pine bark extract (flavangenol (registered trademark)).

  As shown in FIGS. 1-5, it turns out that the proliferation of P.gingivalis is suppressed effectively by combining a pine bark extract and a specific plant material. In particular, coffee tree, linden tree, barley young leaf, and sweet potato young leaf, when combined with pine bark extract, effectively increased the growth-inhibiting action against P. gingivalis, although the effect was weak or not alone. Kuzuka itself showed a growth-inhibiting effect, but it was more effective when combined with a pine bark extract. Therefore, the oral composition of the present invention prevents and / or ameliorates periodontal disease by effectively suppressing the growth of P. gingivalis, and prevents and / or ameliorates bad breath peculiar to periodontal disease. It can be seen that this is an excellent oral care oral composition.

[Example 2] (Manufacture of tablets)
Pine bark extract 12mg
Bodhi tree extract powder (flower origin) 200mg
Sucrose fatty acid ester 350mg
Shellac 10mg
Fragrance 2mg
The mixture consisting of the remainder of the reduced maltose was tableted to produce 4 tablets of 300 mg.

[Example 3] (Manufacture of chewable tablet)
Pine bark extract 1.5mg
Coffee tree extract powder (from raw coffee beans) 150mg
Hydroxypropylcellulose 250mg
Sucrose fatty acid ester 50mg
Aspartame 30mg
Fragrance 3mg
The mixture consisting of the remainder of the reduced maltose was tableted to produce two 500 mg chewable tablets.

[Example 4] (Production of granules)
Pine bark extract 50mg
Barley young leaf powder 2000mg
Fructooligosaccharide 200mg
The mixture consisting of the remainder of the reduced maltose was granulated after mixing to produce 3 g of granules.

[Example 5] (Production of powder)
Pine bark extract 100mg
Sweet potato young leaf powder 1500mg
Tea extract 50mg
The mixture consisting of the dextrin balance was mixed to produce 3 g of powder.

[Example 6] (Manufacture of candy)
Pine bark extract 30mg
Coffee tree extract powder (derived from raw coffee beans) 50mg
Bodhi tree extract powder (from flowers) 20mg
Sweetener (aspartame L-phenylalanine compound,
Acesulfame K, Stevia) 30mg
Fragrance 10mg
One gram of 3 g of candy consisting of the remainder of the reduced maltose starch syrup was prepared according to a conventional method.

[Example 7] (Manufacture of container-packed beverages)
Pine bark extract 200mg
Kuzuhana Extract 35mg
Black tea leaf extract powder 500mg
Sodium ascorbate 50mg
A 350 ml packaged beverage consisting of the remainder of the water was produced according to a conventional method.

  Since the oral composition of this invention can be used as a health food etc. which have a high periodontal disease prevention and / or improvement effect, the industrial usefulness of this invention is high.

Claims (3)

  An oral composition for oral care, comprising a pine bark extract and at least one plant material selected from coffee tree, linden tree, kuzuka, barley young leaf and sweet potato young leaf.   An oral composition for oral care comprising a pine bark extract as an active ingredient, further comprising at least one plant material selected from coffee tree, linden tree, kuzuka, barley young leaf and sweet potato young leaf Oral composition.   An oral composition comprising a pine bark extract and at least one plant material selected from coffee tree and linden tree.