JP2003113104A - Expression promoter of uncoupling protein and composition containing the same - Google Patents

Abstract

PROBLEM TO BE SOLVED: To provide a composition for a medicine, a food and a cosmetic for dieting capable of promoting the expression of an uncoupling protein in a brown adipose cell and promoting the inhibition of excessive fat accumulation. SOLUTION: This composition is obtained by formulating an extracted essence which is separated from a dry fruit of a plant of the genus Zanthoxylum of the family Rutaceae with a polar solvent and capable of promoting the expression of the uncoupling protein having 32 kDa molecular weight and present in the mitochondrial inner membrane in the brown adipose cell and resultantly inhibiting the excessive fat accumulation with the medicine, food and cosmetic. The resultant composition has excellent antiobese effects.

Description

Detailed Description of the Invention

[0001]

Detailed Description of the Invention

[0002]

TECHNICAL FIELD The present invention relates to the citrus family (Rutacea).
e) a technique for providing a composition having a diet effect by promoting the expression of a thermogenic protein having a molecular weight of 32 kilodaltons, which is present in the inner mitochondrial membrane of brown adipocytes, by the extract of Zanthoxylum plant .

[0003]

BACKGROUND OF THE INVENTION Currently, research on obesity is shifting from controlling intake energy to controlling energy consumption. In particular, a thermogenic protein (UCP: uncoupling protein) in brown adipocytes has been attracting attention as being involved in promoting energy consumption. The thermogenic protein has a molecular weight of 32 kilodaltons, and is present in the inner membrane of mitochondria in brown adipocytes between the scapulae, axilla and perirenal. Electron transfer system and AT in mitochondria
P synthesis is more closely coupled to the proton concentration gradient in the inner membrane. Since the thermogenic protein eliminates the proton concentration gradient in a short circuit, when it is activated, the chemical energy of the oxidative substrate is converted into heat without being used for ATP synthesis. The heat generated from it is used to maintain body temperature and dissipate excess energy, and is thus related to energy consumption.

On the other hand, the energy consumed by humans is
The minimum amount of basal metabolic energy required for life support, the energy consumed by skeletal muscle exercise, and the energy actively consumed in response to changes in the internal and external environment (such as cold exposure and overeating) (dietary heat production) is there. Among these, it is difficult to reflect the efforts of the individual such as exercise and lifestyle improvement, and the thermogenic protein is involved in both. Is affected by various factors such as muscle mass, sex, and age, and thermogenic proteins are also considered to be one of them. It is also believed that the majority of these are explained by thermogenic proteins. Therefore, it is thought that by giving an appropriate stimulus and increasing the expression of the thermogenic protein, the energy consumption of the whole body is increased and the obesity is eliminated or prevented.

On the other hand, the dried fruits of the Zanthoxylum plant of the Rutaceae family have been used as crude drugs since ancient times. Zanthoxylum Fruit is the English name for herbal medicine, Zanthoxylum Fruit, and the basis is the matured pericarp of Zanthoxylum piperitum De Candolle, with the seeds separated from the pericarp removed as much as possible. Elsewhere, it is called shosho, and the plant of origin is kahokusansho (Zanthoxylum simulans Hance.).
It is also said to be the fruit of. The main ingredients are α-sanshool and β-sanshool.
It contains essential oils such as l) and is used for anti-inflammatory, diuretic, anthelmintic, stomachic, etc. The properties as a crude drug are
It is a capsule consisting of two to three branches, and each branch has an oblate shape and is split into two pieces with a diameter of about 5 mm. The outer surface of the pericarp is dark yellow-red to dark reddish-brown with numerous sunken spots due to the oil chamber. The inner surface is pale yellowish white. This product has a peculiar fragrance, the taste is spicy and the tongue is paralyzed. When the cross section of this product was examined microscopically, the outer epidermis and the one cell layer in contact with it contained reddish brown tannins, and the pericarp had an oil chamber reaching a diameter of about 500 μm, with spiral spiral ducts in places. The main vascular bundles are scattered, the inner layer consists of a stone cell layer, and the inner epidermal cells are extremely small. Various studies have been carried out on the pharmacological effect of salamander. For example, continuous administration of ether extract of salamander to rats inhibits weight gain, and increases the weight of thyroid and thymus with a small amount and decreases with a large amount. On the contrary, it is said that the adrenal gland and ovary decrease in a small amount and increase in a large amount. In addition, the weight of the pituitary gland decreases and the weight of the testes increases. Ethanol and ether insoluble components of salamander cause a transient decrease in heart rate and an increase in amplitude after amplitude reduction in the isolated heart of cattail, and they show a stringent action up to 100-fold dilution in the rabbit peritoneal vascular perfusion experiment. There is no change in blood pressure or respiration even when injected into the jugular vein of a rabbit, but the extracted intestinal tract is increased in tension with a 50-fold solution and the amplitude is reduced. Moreover, when one of the drops is instilled, a sensory paralysis of the cornea occurs after a few minutes.
In addition, according to the application experiment on the frog, a considerably remarkable local paresthesia effect is recognized. The effects of insecticidal and fish venom of salamander have been known for a long time, but its essential form is a pungent ingredient, and these are known to have a local anesthetic effect.

However, up to now, Rutaceae (Rut
aceae) (Zanthoxylum), for example, Zanthoxylum piperitum, Kahoksansho
(Zanthoxylum simulans Hance.), Fillet salmon (Zant
hoxylum beecheyanum), American salam (Zanthoxy
lum americanum), tomato (Zanthoxylum simula)
na), Inuzansho (Zanthoxylum schinifolium), Crows (Zanthoxylum ailanthoides), Fuyuzansho (Zanthoxylum armatum), Asakuranzansho (Za
It is known that the polar solvent extract of dried fruits of nthoxylum piperium f. Moreover, it has not been known that a pharmaceutical, food or cosmetic composition containing this extract has an antiobesity effect and is suitable for dieting.
Here, diet means to reduce body fat and to lose weight without rebounding.

[0007]

The present invention has been made in view of such a situation, and promotes expression of a thermogenic protein in brown adipocytes, promotes suppression of excessive fat accumulation, and diets. It is an object of the present invention to provide a pharmaceutical composition, a food composition, and a cosmetic composition.

[0008]

[Means for Solving the Problems] In view of such a situation, the present inventors have made diligent research efforts, and as a result, as a result, a plant of the genus Zanthoxylum of the citrus family (Rutaceae), for example, a salmon (Za
nthoxylumpiperitum), Kahok salmon (Zanthoxylum)
simulans Hance.), fillet salmon (Zanthoxylum beech)
eyanum), Zanthoxylum americanu
m), euphorbianthus (Zanthoxylum simulana), Inuzansho (Zanthoxylum schinifolium), crow azalea (Zanthoxylum ailanthoides), fuyuzansho (Zanth
oxylum armatum), Asakura Zansho (Zanthoxylum pi
perium f. inerme), Japanese cherry salmon (Zanthoxy)
lum piperium f. brevispinum) dried fruit polar solvent extract extract suppresses excess fat accumulation by promoting the expression of thermogenic protein in brown adipocytes.
It has been found that a composition having an excellent diet effect can be provided by adding it to a drug, a food and a cosmetic, and the invention has been completed. That is, the present invention relates to the following technology. (1) Zanthanthus plant of the Rutaceae family (Zanth
oxylum) extract is contained in the composition. (2) The plants of the genus Zanthoxylum are Zanthoxylum piperitum and Zantho.
xylum simulans Hance.), fillet salmon (Zanthoxylum
beecheyanum), American salam (Zanthoxylum ame)
ricanum), tomato (Zanthoxylum simulana),
Inuzanzou (Zanthoxylum schinifolium), Crowszaw (Zanthoxylum ailanthoides), Fuyuzansho
(Zanthoxylum armatum), Asakura Zansho (Zanthoxylum armatum)
lum piperium f. inerme), Yamasakura Sakura Show (Za
nthoxylum piperium f. brevispinum), selected from the group consisting of one or two or more thereof. (3) Zanthoxylum is a plant of the genus Zanthoxylum, and Zanthoxylum piperitum and Zantho
xylum simulans Hance.), Plum Blossom (Zanthox
ylum piperium f. inerme), fillet salmon (Zanthoxyl)
um beecheyanum), American salam (Zanthoxylum am
ericanum), peach salamander (Zanthoxylum simulana) and dog salamander (Zanthoxylum schinifolium), selected from one or two or more kinds thereof, (1) or (2). (4) The composition according to any one of (1) to (3), wherein the extract is an extract of a mature dried fruit with a polar solvent. (5) The composition according to any one of (1) to (4), which has an effect of suppressing excess fat accumulation. (6) Zanthanthus plants of the Rutaceae family (Zanth
(oxylum) extract, a thermogenic protein expression promoter. (7) Zanthoxylum is a plant of the genus Zanthoxylum, and Zanthoxylum piperitum and Zantho
xylum simulans Hance.), fillet salmon (Zanthoxylum
beecheyanum), American salam (Zanthoxylum ame)
ricanum), tomato (Zanthoxylum simulana),
Inuzanzou (Zanthoxylum schinifolium), Crowszaw (Zanthoxylum ailanthoides), Fuyuzansho
(Zanthoxylum armatum), Asakura Zansho (Zanthoxylum armatum)
lum piperium f. inerme), Yamasakura Sakura Show (Za
(nthoxylum piperium f. brevispinum) selected from the group consisting of one or two or more thereof, wherein the thermogenic protein expression promoter according to (6). (8) Zanthoxylum is a plant of Zanthoxylum, Zanthoxylum piperitum, and Zantho
xylum simulans Hance.), Plum Blossom (Zanthox
ylum piperium f. inerme), fillet salmon (Zanthoxyl)
um beecheyanum), American salam (Zanthoxylum am
ericanum), euphorbianthus (Zanthoxylum simulana), and dog salamander (Zanthoxylum schinifolium), selected from one or two or more species, and the thermogenic protein expression promoter according to (6) to (7). (9) The thermogenic protein expression promoter according to any one of (6) to (8), wherein the extract is an extract of a mature dried fruit with a polar solvent. (10) The thermogenic protein expression promoter according to any one of (6) to (9), which has an effect of suppressing excess fat accumulation. (11) A thermogenic protein expression promoter according to any one of claims 6 to 10, which is a food, a drug, or a cosmetic (12) (6) to (11). Characterized in that it contains a thermogenic protein expression promoter according to
A composition for food, medicine, or cosmetics. (13) It is for diet, (1
The composition for foods, pharmaceuticals, or cosmetics according to 2). Hereinafter, the present invention will be described focusing on the embodiments.

[0009]

BEST MODE FOR CARRYING OUT THE INVENTION (1) Thermogenic protein expression promoting agent of the present invention The thermogenic protein expression promoting agent of the present invention is
aceae) and an extract of Zanthoxylum. Zanthoxylum includes Zanthoxylum piperitum and Zantho salmon.
xylum simulans Hance.), fillet salmon (Zanthoxylum
beecheyanum), American salam (Zanthoxylum ame)
ricanum), tomato (Zanthoxylum simulana),
Inuzanzou (Zanthoxylum schinifolium), Crowszaw (Zanthoxylum ailanthoides), Fuyuzansho
(Zanthoxylum armatum), Asakura Zansho (Zanthoxylum armatum)
lumpiperium f. inerme), Yamasakura Sakura Show (Zan
thoxylum piperium f. brevispinum) and the like, and is characterized by being selected from one or more of these plant extracts. Any substance that promotes the expression of the thermogenic protein of the present invention is included. Therefore, any of these plants can be used as the extract of the present invention. However, in order to extract the extract of the thermogenic protein expression promoter, among others, salmon (Zanthoxylum piperitu
m), Kahoku Sansho (Zanthoxylum simulans Hance.),
Zanthoxylum piperium f. Inerm
e), fillet salamander (Zanthoxylum beecheyanum), American salamander (Zanthoxylum americanum), tow salamander (Zanthoxylum simulana) and dog salamander (Zant
hoxylum schinifolium) is preferably used. this is,
This is because many substances that promote the expression of the thermogenic protein are contained. Here, the extract refers to the plant itself of such a plant, a processed product obtained by processing the plant such as drying or shredding, crushing, an extract obtained by extracting the plant or the processed product with a solvent, a solvent of the extract. It means a generic term for the removed solvent-removed product, the extract or the purified fractionated product obtained by purifying the solvent-removed product by column chromatography or liquid-liquid extraction.
The extract can be extracted from the whole plant grass. Among these, as a substance that promotes the expression of the thermogenic protein of the present invention, in the case of a plant of the citrus family Rutaceae (Zanthoxylum), a solvent extract of dried fruits or a solvent-removed product thereof is preferable. Examples thereof include polar solvents, and polar solvents are particularly preferable. Examples of such a polar solvent include water, alcohols such as ethanol, methanol, 1,3-butanediol and propylene glycol, esters such as ethyl acetate and methyl formate, ketones such as acetone and methyl ethyl ketone, chloroform and One or more selected from halogenated hydrocarbons such as methylene chloride, nitriles such as acetonitrile, and ethers such as diethyl ether and tetrahydrofuran can be preferably exemplified. Of these, water and alcohol are particularly preferable.
To prepare such an extract, 1 to 10 times the amount of the solvent is added to the plant or its processed product, and the mixture may be immersed for several days at room temperature and for several hours at a temperature near the boiling point. After that, the insoluble matter can be removed by filtration and the solvent can be removed by concentration under reduced pressure or freeze-drying, if necessary. In the case of dried fruits of Zanthoxylum, it is particularly preferable as an essence to extract 50% ethanol, filter, concentrate under reduced pressure, and optionally freeze-dry. that is,
This is because the dried fruit portion contains a large amount of a substance that promotes the expression of the thermogenic protein, which is particularly preferable. The thus obtained plant of the genus Ranaceae (Ranthaceae), which is a thermogenic protein expression promoter of the present invention (Zanthoxyl
(um) dried fruit extract has an excellent effect of promoting the expression of thermogenic proteins, thereby promoting energy consumption,
Has an anti-obesity effect. The thermogenic protein is present in the inner membrane of mitochondria in brown adipocytes between the scapulae, axilla and perirenal, and has a molecular weight of 32 kilodaltons. The mitochondrial electron transfer system and ATP synthesis are tightly coupled to the proton concentration gradient in the inner membrane, and the thermogenic protein eliminates the proton concentration gradient in a short-circuiting manner, so that it is activated. The chemical energy of the oxidative substrate is not used for ATP synthesis and is converted to heat. And
The heat generated from it is used to maintain body temperature and dissipate excess energy, and is thought to be related to energy consumption in this way. Further, since such an extract also contains a substance having an inhibitory effect on excess fat accumulation, a composition having an excellent diet effect by incorporating it into a drug, a food or a cosmetic for the purpose of such an effect. Can provide things. Since such an action works synergistically or additively with the thermogenic protein expression promoter of the present invention, when these extracts are contained in a cosmetic, the present invention also provides a slimming cosmetic. Belong to the technical scope of.

(2) Method for measuring fat mass around epididymis in mice A group of nine 8-week-old male ddY mice was added to a basic feed MF containing 20% of purified lard as a test substance, and a citrus family as a test substance. (Rutaceae) plant (Zanthox
5% of 50% ethanol extract powder of dried fruits of
They were added and the mice were allowed to freely ingest for 4 weeks. Then, the adipose tissue around the epididymis is collected, weighed, and weighed 100 g.
The tissue weight per unit was calculated. The test substance group was tested at the same time as the vehicle group (control), and the value of the vehicle group was set to 1
The relative value when 00 was set was used to confirm the presence or absence of an obesity-suppressing action by increasing or decreasing the epididymal fat mass.

(3) Experimental method for promoting expression of thermogenic protein in interscapular brown adipose tissue of mice A group of 9 8-week-old ddY male mice was added, and 20% of purified lard was added to the basic feed MF as a feed. As a test substance, a plant of the genus Zanthox of the Rutaceae family (Zanthox
5% of 50% ethanol extract powder of dried fruits of
They were added and the mice were allowed to freely ingest for 4 weeks. Then, the interscapular brown adipose tissue was collected and centrifuged to obtain a crude mitochondrial fraction. The protein concentration in the fraction was 5 μg /
After diluting to 10 μL, the thermogenic protein was detected by Western blotting. The detected band intensities were quantified, and the relative thermogenic protein content was calculated when the value of the vehicle group was 100.

(4) Composition having anti-obesity action of the present invention The composition having anti-obesity action of the present invention is a composition of Rutacaceae (Rutac family).
eae) is an extract of a Zanthoxylum plant. The above-mentioned extract having a thermogenic protein expression promoter is effective for the improvement, prevention and treatment of humans with obesity and excessive calories, and by oral administration or external application, it reduces body fat and makes skin lean without rebound. It is possible to obtain an excellent improvement effect. Rutaceae (Rutacea) having the thermogenic protein expression promoter of the present invention
The e) dried fruit extract of Zanthoxylum can be administered to animals or humans as it is or together with a conventional pharmaceutical carrier. A preferred effective daily dose for the food and pharmaceutical composition of the present invention is 0.01 to 100 mg / kg body weight / day as a dry extract,
A more preferable dosage is 0.5 to 50 mg / kg body weight / day. The dosage form is not particularly limited and may be appropriately selected and used as needed. Oral preparations such as tablets, capsules, granules, fine granules and powders, parenteral preparations such as injections and suppositories may be used. Can be mentioned. The above formulations are manufactured by a conventional method. Any of the commonly known optional ingredients may be used as the thermogenic protein promoter of the present invention (= Rutaceae plant of the genus Ranaceae (Zan
Thioxylum) dried fruit extract) can be blended to such an extent that the physiological activity is not impaired. As such optional ingredients, sucrose, excipients such as lactose, starch, binders such as gelatin, sodium carboxymethyl cellulose, disintegrants such as carboxymethyl cellulose calcium, soybean lecithin, surfactants such as sucrose fatty acid ester, talc,
Lubricants such as waxes, light anhydrous silicic acid, flow promoters such as dried aluminum hydroxide gel, physiological saline, diluents such as glucose aqueous solution, flavoring agents, colorants, bactericides, preservatives,
Examples include fragrances. Further, the cosmetic composition referred to in the present invention is a general term for compositions that are externally applied to the skin, and a skin external medicine containing a patch and a cosmetic containing a detergent can be preferably exemplified. Then, it is particularly preferable that it is a cosmetic. This is because the thermogenic protein expression promoter of the present invention has high safety and a mild action. Involved in the thermogenic protein expression promoter of the present invention,
In the skin external preparation for slimming, the preferable content of the thermogenic protein expression promoter is relative to the total skin external preparation,
The amount is 0.001% by weight to 10% by weight, and more preferably 0.01% by weight to 5% by weight. This is because if the amount is too small, the thermogenic protein expression promoting action may not be exhibited, and if the amount is too large, the effect may reach the limit and the freedom of other prescription ingredients may be impaired.

The skin external preparation for slimming, in which the thermogenic protein expression promoter of the present invention is involved, may contain, in addition to the above-mentioned essential components, any component usually used in cosmetics and skin external medicines. As such an optional component, for example,
Hydrocarbons such as squalane, petrolatum, microcrystalline wax, jojoba oil, carnauba wax, esters such as octyldodecyl oleate, olive oil,
Triglycerides such as beef tallow, coconut oil, stearic acid,
Fatty acids such as oleic acid and lithinoleic acid, higher alcohols such as oleyl alcohol, stearyl alcohol and octyldodecanol, anionic surfactants such as sulfosuccinate and sodium polyoxyethylene alkyl sulfate, and amphoteric surfactants such as alkyl betaine salts Kind,
Cationic surfactants such as dialkyl ammonium salts,
Sorbitan fatty acid ester, fatty acid monoglyceride,
Nonionic surfactants such as polyoxyethylene adducts, polyoxyethylene alkyl ethers and polyoxyethylene fatty acid esters, polyhydric alcohols such as polyethylene glycol, glycerin and 1,3-butanediol, thickening / gel It may contain an agent, an antioxidant, an ultraviolet absorber, a coloring agent, a preservative, a powder and the like.

[0014]

EXAMPLES The present invention will be described in more detail below with reference to Examples, but it goes without saying that the present invention is not limited to these Examples.

<Example 1> An extract of dried fruits of the genus Ranaceae (Zanthoxylum) of the Rutaceae family was prepared by adding 5 L of 50% ethanol to 500 g of dried fruits, extracting at room temperature for 1 week, and then filtering. The insoluble matter was removed, the mixture was further concentrated under reduced pressure, and then freeze-dried. And the salmon extract 1 which is the thermogenic protein expression promoter of this invention
Got

<Example 2> An extract of dried fruits of Zanthoxylum of the citrus family (Rutaceae) was added to 500 g of dried fruits with 5 L of 50% ethanol and heated at 90 ° C for 2 hours while stirring. And after cooling to room temperature,
The insoluble matter was removed by filtration, the mixture was further concentrated under reduced pressure, and then freeze-dried. And the extract 2 of the salamander which is a thermogenic protein expression promoter of this invention was obtained.

<Examples 3 to 9> Effect of dried fruit extract of Zanthoxylum plant of the present invention on peripheral epididymal fat weight of mice A group of 9-week-old male ddY mice, As a feed, 20% of purified lard was added to the basic feed MF, and as a test substance, a plant of the genus Zanthox of the Rutaceae family (Zanthox)
5% of 50% ethanol extract powder of dried fruits of
They were added and the mice were allowed to freely ingest for 4 weeks. The extract used here was extracted and treated by the method of Example 2. As an extract of dried fruits of Zanthoxylum, Example 3: Zanthoxylum piperitum,
Example 4: Kaohokusansho (Zanthoxylum simulans Ha
nce.), Example 5: Zanthoxylum pi
perium f. inerme), Example 6: Fillet salmon (Zantho)
xylum beecheyanum), Example 7: American salamander (Z
anthoxylum americanum), Example 8: Tosamou (Z)
anthoxylum simulana) and Example 9: Indosansho
(Zanthoxylum schinifolium) was used. Then, adipose tissue around the epididymis was collected, weighed, and the tissue weight per 100 g of body weight was calculated. The test substance group was tested at the same time as the vehicle group (control), and the value of the vehicle group was set to 1
The relative value when 00 was set was used to confirm the presence or absence of an obesity-suppressing action by increasing or decreasing the epididymal fat mass. As shown in Table 1, as compared to the vehicle group, a plant of the genus Prunus (Zanthox)
(ylum) dried fruit extract had a low adipose tissue weight ratio around the epididymis. This shows that the extract has an obesity-suppressing effect.

[0018]

[Table 1]

<Examples 10 to 16> Plants of the genus Rutaceae of the present invention (Zanth)
Effect of dried fruit extract of oxylum) on the expression of thermogenic protein in interscapular brown adipose tissue of mice A group of 9 8-week-old male ddY mice and 20% purified lard as basic feed MF In addition, as a test substance, a plant of the genus Ranaceae (Rutaceae) in the genus Zanthox
5% of 50% ethanol extract powder of dried fruits of
They were added and the mice were allowed to freely ingest for 4 weeks. The extract used here was extracted and treated by the method of Example 2. Then, the interscapular brown adipose tissue was collected and centrifuged to obtain a crude mitochondrial fraction. After diluting the protein concentration in the fraction to 5 μg / 10 μL, detection of thermogenic protein by Western blotting was performed. The detected band intensities were quantified, and the relative thermogenic protein content was calculated when the value of the vehicle group was 100. As an extract of dried fruits of the genus Zanthoxylum, Example 10: Zanthoxylum piperitum, Example 11: Zanthoxylum simulans Hanc
e.), Example 12: Zanthoxylum pi
perium f. inerme), Example 13: fillet salmon (Zant
hoxylum beecheyanum), Example 14: Zanthoxylum americanum, Example 15: Zanthoxylum simulana, and Example 16: Zanthoxylum schinifolium. As shown in Table 2, as compared to the vehicle group, Zanthoxylum plants (Zan
The expression of the thermogenic protein was promoted in the one to which the dried fruit extract of thoxylum) was added. From this,
It can be seen that the extract has an obesity suppressing effect.

[0020]

[Table 2]

<Examples 17 to 19> Health foods were prepared according to the formulations shown in Table 3. That is, the prescription ingredients were tumbling-phase granulated with 10 parts by weight of water and compressed into tablets to obtain a health food in the form of tablets. All of these showed excellent preventive and improving effects on obesity. The unit of numerical values in the table is parts by weight.

[0022]

[Table 3]

<Examples 20 to 22> Health foods were prepared according to the formulations shown in Table 4. That is, the prescription ingredients were stirred and solubilized to obtain a health food as a drink preparation. All of these showed excellent preventive and improving effects on obesity.
The unit of numerical values in the table is parts by weight.

[0024]

[Table 4]

<Examples 23 to 25> According to the formulations shown in Table 5, the drugs for injection were prepared by dissolving with stirring at room temperature. The unit of numerical values in the table is parts by weight. It had the effect of lowering the body fat percentage.

[0026]

[Table 5]

Example 26 A lotion was prepared according to the following formulation. That is, the prescription ingredients were stirred and solubilized at room temperature to obtain a lotion. The skin lotion was used by 3 panelists 1 group suffering from obesity, and was applied to the abdomen twice a day for 1 month in the morning and evening, and the effect of the slimming effect was evaluated. The evaluation standard is
Rating 2: Significant improvement, Rating 1: Clear improvement, Rating 0.5:
Slight improvement, score 0: No improvement standard. The average score was 0.88. It was found that the lotion containing the extract of dried fruit of salamander having the thermogenic protein expression promoting effect of the present invention has an abdominal slimming effect. 1. Dried fruit extract of salamander of Example 1. 0 parts by weight 1,3 butanediol 5.0 parts by weight glycerin 3.0 parts by weight sodium citrate 0.1 parts by weight methylparaben 0.2 parts by weight ethanol 8 parts by weight water 82.7 parts by weight

Example 27 A cream was prepared according to the following formulation. That is, a, b and c are each 80 ° C.
After heating and melting, the mixture was gradually added to b, and after further adding c to emulsify, the emulsified particles were homogenized with a homomixer and cooled to obtain a cream. This cream had an excellent slimming effect. B) Squalane 10 parts by weight Cetanol 3 parts by weight Sorbitan sesquistearate 2 parts by weight Polyoxyethylene (20) behenyl ether 2 parts by weight Vitamin A acid 1 part by weight b) 1,3-butanediol 5 parts by weight Sun of Example 2 Dried fruit extract 1 part by weight Carboxyvinyl polymer 0.3 parts by weight Water 40 parts by weight c) Water 37.3 parts by weight Potassium hydroxide 0.2 parts by weight

[0029]

INDUSTRIAL APPLICABILITY According to the present invention, a polar solvent-extracted extract of dried fruits of the citrus family (Rutaceae) of the genus Zanthoxylum is excessive by promoting the expression of thermogenic proteins in brown adipocytes. A composition having an excellent diet effect can be provided by suppressing fat accumulation and incorporating it into a pharmaceutical product, a food product and a cosmetic product.

[0030]

   ─────────────────────────────────────────────────── ─── Continued front page    (72) Inventor Masaki Kamakura             27-1 Takashimadai, Kanagawa-ku, Yokohama-shi, Kanagawa             Pola Chemical Industry Co., Ltd. Pola Yokohama Laboratory             Within F term (reference) 4B018 LB08 LE03 MD52 ME01 MF01                       MF06                 4C083 AA111 AA112 AC022 AC072                       AC102 AC122 AC182 AC302                       AC442 AC482 AD092 AD622                       CC02 CC04 CC05 EE11 FF01                 4C088 AB62 AC04 BA08 CA06 MA17                       MA35 MA52 MA66 NA14 ZA70

Claims (13)

[Claims] 1. A plant of the genus Ranaceae belonging to the genus Rutaceae.
(Zanthoxylum) containing an extract,
Composition. 2. The plant of the genus Zanthoxylum is Zanthoxylum piperitum, or Kahoku Sansho.
(Zanthoxylum simulans Hance.), Fillet salmon (Zant
hoxylum beecheyanum), American salam (Zanthoxy
lum americanum), tomato (Zanthoxylum simula)
na), Inuzansho (Zanthoxylum schinifolium), Crows (Zanthoxylum ailanthoides), Fuyuzansho (Zanthoxylum armatum), Asakuranzansho (Za
nthoxylum piperium f. inerme) and Yamasaki sakurazansho (Zanthoxylum piperium f. brevispinum) are selected from one kind or two or more kinds.
The composition according to. 3. The plant of the genus Zanthoxylum is Zanthoxylum piperitum, or Kahoku Sansho.
(Zanthoxylum simulans Hance.), Asakura Zansho
(Zanthoxylum piperium f. Inerme), fillet salamander (Z
anthoxylum beecheyanum), American salamander (Zantho
xylum americanum), peach salamander (Zanthoxylum simu)
lana) and Inuzansho (Zanthoxylum schinifolium)
3. The composition according to claim 1, which is selected from one or more of 4. The composition according to claim 1, wherein the extract is an extract of mature dried fruits with a polar solvent. 5. The composition according to any one of claims 1 to 4, which has an effect of suppressing excess fat accumulation. 6. A plant of the genus Ranaceae of the citrus family Rutaceae.
(Zanthoxylum) extract, a thermogenic protein expression promoter. 7. The plant of the genus Zanthoxylum is Zanthoxylum piperitum, Kashoku Sansho.
(Zanthoxylum simulans Hance.), Fillet salmon (Zant
hoxylum beecheyanum), American salam (Zanthoxy
lum americanum), tomato (Zanthoxylum simula)
na), Inuzansho (Zanthoxylum schinifolium), Crows (Zanthoxylum ailanthoides), Fuyuzansho (Zanthoxylum armatum), Asakuranzansho (Za
nthoxylum piperium f. inerme) and Yamasaki sakurazansho (Zanthoxylum piperium f. brevispinum) are selected from one kind or two or more kinds.
The thermogenic protein expression promoter according to item 1. 8. The plant of the genus Zanthoxylum is Zanthoxylum piperitum, Kashoku Sansho.
(Zanthoxylum simulans Hance.), Asakura Zansho
(Zanthoxylum piperium f. Inerme), fillet salamander (Z
anthoxylum beecheyanum), American salamander (Zantho
xylum americanum), peach salamander (Zanthoxylum simu)
lana) and Inuzansho (Zanthoxylum schinifolium)
8. The thermogenic protein expression promoter according to claim 6, which is selected from one or more of 9. The thermogenic protein expression promoting agent according to claim 6, wherein the extract is an extract of a mature dried fruit with a polar solvent. 10. The thermogenic protein expression promoting agent according to claim 6, which has an effect of suppressing excess fat accumulation. 11. The thermogenic protein expression promoter according to any one of claims 6 to 10, which is a food product, a pharmaceutical product, or a cosmetic product. 12. A composition for foods, pharmaceuticals, or cosmetics, which comprises the thermogenic protein expression promoter according to any one of claims 6 to 11. 13. The composition for foods, pharmaceuticals, or cosmetics according to claim 12, which is for dieting.