FR3085839A1 - USE OF PHYTOSTEROLS - Google Patents

Abstract

The present invention relates to the cosmetic use of phytosterols to protect and / or restore the skin microbiota and / or to prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota. It also relates to a cosmetic care process comprising the topical application to at least one skin area, advantageously of the scalp, of phytosterols to protect and / or restore the cutaneous microbiota and / or to prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota. Finally, it relates to phytosterols for their use in the treatment and / or prevention and / or reduction of the occurrence of a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of acne, psoriasis and bacterial and / or fungal infections, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, erythyma erysipelas, yeast infections such as candidiasis or dermatophytosis such as ringworm and / or scabies pigment and / or acne scars and their combinations and / or in the treatment and / or prevention of superinfection of wounds and / or in the prevention of spots.

Description

The present invention relates to the use of phytosterols, preferably extracted from vegetable oil, preferably rapeseed, to protect and / or restore the microbiota of the skin (in particular when the latter is altered for example by aggressive agents) and / or prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of the deterioration of the microbiota of the skin, in particular in cosmetic or pharmaceutical compositions, in particular dermatological.

The microbiota represents all of the microorganisms found in a given environment or ecosystem, such as the skin, the intestine and the oral cavity.

This microbiota is composed in particular of bacteria, fungi, which can be resident (established over a long period) in the ecosystem or transient. Different modes of interaction with the host organism that carry them arise according to their mode of presence. Thus in resident microorganisms, we can distinguish commensals, which do no harm to the host and which are increasingly described as being beneficial by the protection which they provide to the host. There are also mutualists who obtain mutual benefit with the host and certain opportunistic pathogens. In transient microorganisms, we can distinguish commensals, or opportunistic pathogens which under certain conditions prove to be dangerous for the host organism.

The skin is the largest organ in our body. It protects our organism from the external environment and from the dangers linked to various environmental factors such as UV rays, pollution, climatic elements, and from all events linked to the use of hygiene products, care, maintenance and medication. The microbiota of the skin constitutes a defense of the skin against these various aggressions, in particular against invasive pathogens. Thus the therapeutic elimination of the skin microbiota such as by antibiotic or disinfection promotes pathogenic infections of the skin.

The microbiota of the skin is exposed to various aggressions, in particular by the aggressive agents contained in cosmetic and / or pharmaceutical, hygiene and / or maintenance products such as, for example, detergents, surfactants, antiseptic products and / or bacteriostats which deteriorate the composition and / or the distribution of the microbiota. It results from this alteration of the microbiota of the skin unsightly and / or unpleasant and / or uncomfortable manifestations and can sometimes lead to the development of real skin pathologies such as acne, psoriasis and / or bacterial and / or mycotic diseases.

The reduction in the contents of aggressive agents contained in the compositions or their replacement by agents of a less aggressive nature makes it possible to partially solve the problem but is not sufficient for skins made intolerant and is above all to the detriment of the effectiveness of said compositions. In addition, for certain agents with a disinfectant, preservative or antibiotic function, the compromise between efficacy and preservation of the microbiota is almost impossible.

There is thus a need to have an active agent capable of protecting and / or restoring the skin microbiota, in particular in the presence of aggressive agents of the skin microbiota, in particular those contained in detergent products, hygiene products (including disinfectants ), cosmetic product (including make-up) and / or pharmaceutical product (including antibiotic and / or antifungal compositions), in particular dermatological. There is also a need for a new active agent capable, moreover, of preventing and / or reducing unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota.

The present invention thus aims to solve these needs with a new protective agent for the skin microbiota, useful for offsetting the negative effects of aggressive agents for the skin microbiota, in particular the agents contained in topical compositions.

According to the present invention, it has in fact been discovered in a particularly surprising and unexpected manner that phytosterols make it possible to solve all of these technical problems. The phytosterols according to the invention indeed make it possible to protect and / or restore the cutaneous microbiota, in particular when the latter is altered by aggressive agents. The phytosterols according to the invention also make it possible to prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota.

Phytosterols notably act as a skin prebiotic in that they increase the growth and / or activity of the skin microbiota.

Furthermore, phytosterols, in particular rapeseed, have the advantage of having a soothing effect, which makes it possible to provide complete action for intolerant and / or sensitive and / or sensitized skin.

The phytosterols according to the invention also have the advantage of being able to be applied to the skin sequentially or concomitantly with the aggressive agents of the skin microbiota.

The phytosterols according to the invention are phytosterols of natural origin, in particular plant oil phytosterols, preferably rapeseed oil phytosterols, in particular phytosterols extracted from rapeseed oil.

Phytosterols are known for their anti-inflammatory properties and their actions on the gut microbiota are the subject of studies.

None of the known properties of phytosterols nevertheless suggested their effect to protect and / or restore the skin microbiota whose nature is different from the intestinal microbiota and / or prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of alteration of the skin microbiota as discovered in the present invention.

Some of these properties of the phytosterols according to the invention will in particular be demonstrated in more detail in the examples provided below.

A subject of the present invention is therefore the cosmetic use of phytosterols, preferably vegetable oil phytosterols, preferably rapeseed oil phytosterols, in particular phytosterols extracted from rapeseed oil, to protect and / or restore the skin microbiota and / or prevent and / or reduce the unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota, preferably by aggressive agents of the skin microbiota.

The present invention also relates to phytosterols, preferably phytosterols from vegetable oil, preferentially phytosterols from rapeseed oil, in particular phytosterols extracted from rapeseed oil, for their use alone or in a composition. pharmaceutical, in particular dermatological, in the treatment and / or prevention and / or reduction of the occurrence of a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of skin infections, in particular bacterial infections and / or fungal, acne and / or psoriasis, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, ecthyma erysipelas, yeast infections such as candidiasis or dermatophytosis such as ringworm and / or scabies and / or acne scars and their combinations and / or in the treatment t and / or the prevention of superinfection of wounds and / or in the prevention of blemishes.

A subject of the present invention is also the use according to the invention of phytosterols in combination with at least one aggressive agent of the skin microbiota, in particular in a detergent composition, a hygiene composition (including disinfectants), a cosmetic composition (including make-up) and / or pharmaceuticals (including antibiotic and / or antifungal compositions), in particular dermatological, to protect and / or restore the cutaneous microbiota, and / or to prevent and / or reduce unsightly and / or unpleasant manifestations and / or uncomfortable alteration of the skin microbiota by aggressive agents of the skin microbiota contained in said composition.

Another subject of the present invention is the use of phytosterols to protect the skin microbiota from the effects of an aggressive agent of the skin microbiota, in particular contained in a topical composition, in particular a hygiene product or a cosmetic composition, and / or to make the skin more tolerant of this aggressive agent of the microbiota. In particular, the phytosterols are applied concomitantly or sequentially with the aggressive agent of the skin microbiota, more particularly the phytosterols and the aggressive agent of the skin microbiota are contained in the same composition.

The present invention finally relates to a cosmetic care and / or treatment process comprising the topical application of phytosterols according to the invention, in particular in the form of a composition containing it, concomitantly and / or following the application of an aggressive agent of the skin microbiota topically, on at least one area of skin, advantageously of the scalp, to protect and / or restore the skin microbiota and / or to prevent and / or reduce unsightly and / or unpleasant manifestations and / or uncomfortable damage to the skin microbiota.

The present invention also relates to the use of phytosterols, preferably vegetable oil phytosterols, preferably rapeseed oil phytosterols, in particular phytosterols extracted from rapeseed oil, as skin prebiotic .

For the purposes of the present invention, the term "skin prebiotic" means any product which promotes the growth and / or activity of bacteria of the skin microbiota.

The subject of the present invention is the cosmetic use of phytosterols, preferably vegetable oil phytosterols, preferably rapeseed oil phytosterols (Brassica campestris), in particular phytosterols extracted from rapeseed oil, for protect and / or restore the skin microbiota.

Advantageously, the phytosterols according to the invention are extracted from rapeseed oil by conventional techniques and can be purified according to techniques known to those skilled in the art. Preferably, rapeseed oil is obtained by cold or hot pressing of the seeds and purified by transesterification. According to an advantageous mode, the phytosterols are then concentrated by distillation. Crystals of these phytosterols are obtained by cooling, and purified by successive stages of filtration and washing with solvents. The washing solvents are removed by heating under vacuum, and the molten phytosterols are obtained in the form of powder by passage over a priller jet, to obtain purified phytosterols (> 95%).

Advantageously, the phytosterols according to the invention contain at least beta-sitosterol, campesterol, brassicasterol or their mixtures, preferably two phytosterols chosen from beta-sitosterol, campesterol and / or brassicasterol, still preferably contain beta -sitosterol, campesterol and brassicasterol. Even more preferably, the phytosterols according to the invention comprise:

from 30 to 70%, preferably from 40 to 60% of beta-sitosterol, by weight relative to the total weight of phytosterols from 25 to 60% of campesterol, preferably from 25% to 40%, by weight relative to the total weight of phytosterols from 5 to 25% of brassicasterol, preferably from 7 to 18%, by weight relative to the total weight of phytosterols.

The phytosterols can be used as such according to the invention. According to an advantageous mode, the phytosterols according to the invention are mixed with a fatty alcohol.

According to an advantageous mode, the phytosterols according to the invention are combined with at least one fatty alcohol, as described in patent application DE1995125822A1 (Henkel). This has the advantage of lowering the melting point of phytosterols. The fatty alcohol thus has the formula RI-OH, with RI denoting a C6-22 alkyl or alkenyl group, linear or branched, and containing 0, 1.2 or 3 double bonds. Preferably, the weight ratio of phytosterols / fatty alcohol is between 40/60 and 60/40, preferably between 45/55 and 55/45, even more preferably it is 50/50.

Preferably, the fatty alcohol is chosen from: caprylic alcohol, hexanol, stearyl alcohol, isostearyl, cetyl, 2-ethylhexyl, lauryl, isotridecylic, myristylic, oleic, linoleic, linolenic, cetearyl and mixtures thereof.

Preferably, the alcohol is cetearyl alcohol (INCI: cetearyl alcohol) and is added at a weight ratio: 1/1 relative to the phytosterols.

The fatty alcohol / phytosterol mixture according to the invention is preferably heated above the melting point and atomized under cold air flow (priling).

According to the invention, the term "skin microbiota" means commensal and mutualistic skin bacteria.

The commensal and mutualistic skin bacteria are chosen from those:

of the actinobacteria phylum comprising the family of Corynebacteriacease, in particular the genus Corynebacterium, bacteria of the family Micrococcaceae, in particular the genera Micrococcus and / or Kocuria, bacteria of the family of Propionibacteriaceae with the genus propionibacterium, bacteria of the family Brevibacteriaceae including the genus Brevibacterium.

Bacteria of the Proteobacteria Phylum including those of the family Rhodobacteriaceae in particular the genera Paracoccus and / or Amaricoccus, those of the family of Acetobacteraceae in particular the genus Roseomonas, of the family of Caulobacteraceae in particular the genus Brevundimonas, of the family of Moraxellaceae in particular the genera Enhydrobacter and or Acinetobacter.

of the firmicutes phylum with bacteria of the staphylococcaceae family with the genus staphylococcus in particular Staphylococcus hominis, S. warneri, S. capitis, S. epidermidis, preferably Staphylococcus epidermidis, those of the family Bacilliaceae with the genus Bacillus. and those lactobacilliaceae, in particular the genus Lactobacillus.

Preferably, the commensal and mutualistic skin bacteria are chosen from the genus Corynebacterium, the genus Micrococcus, the genus Kocuria, the genus propionibacterium, the genus Brevibacterium, the genus Paracoccus, the genus Amaricoccus, the genus Roseomonas, the genus Brevundimonas, the genus Enhydrobacter , the genus Acinetobacter, Staphylococcus hominis, S. warneri, S. capitis, S. epidermidis, preferably Staphylococcus epidermidis, the genus Bacillus, the genus Lactobacillus and their mixtures.

The opportunistic pathogenic skin bacteria are chosen from those:

from the proteobacteria phylum, from the family of Enterobacteriaceae, in particular the genus Pantoea, and / or from the family of Pseudomonaceae, in particular from the genus Pseudomonas.

- the phylum of firmicutes with the family of staphylococcaceae in particular the genus Staphylococcus in particular Staphylococcus aureus, and with the family of Streptococcaceae, in particular Streptococcus pyogenes.

- the phylum of Actynobacteria comprising the family of Proprionibacteriaceae, in particular Propionibacterium acnes,

Preferably, the pathogenic skin bacteria are chosen from the genus Pantoea, the genus Pseudomonas, Staphylococcus aureus, Streptococcus pyogenes and Propionibacterium acnes. According to a preferential mode, the pathogenic cutaneous bacteria are Staphylococcus aureus, and / or Propionibacterium acnes.

By "protecting and / or restoring the skin microbiota" is meant keeping constant and / or increasing the content of one or more strains of skin commensal and mutualistic bacteria and / or preventing the proliferation and / or decreasing the content of pathogenic opportunistic bacteria.

Several methods can be used to measure the content of the strains of bacteria in the skin, including a count of the colonies present on the skin, an in vitro measurement by optical density after recovery of samples containing the strains or a measurement by PCR. . Advantageously, the content of the bacteria is measured in vitro by optical density after recovery of samples containing the strains. The measurement method can also include the extraction of microbial DNA by a mechanical and chemical double action, their quantification by fluorescence and then their analysis by metasequencing of a fragment of 16S ribosomal RNA as indicated in Example 2. This measurement is carried out on the samples treated with the phytosterols according to the invention in the presence or not of an aggressive agent of the skin microbiota.

The protection and / or restoration of the skin microbiota can be measured by concomitant or sequential application of an aggressive agent of the skin microbiota, preferably Sodium Lauryl Sulfate (SLS) in particular as described in Example 2.

The present invention also relates to the cosmetic use of phytosterols, preferably phytosterols from vegetable oil, preferably phytosterols from rapeseed oil, in particular phytosterols extracted from rapeseed oil, for preventing and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of damage to the skin microbiota.

The term “unsightly and / or unpleasant and / or uncomfortable manifestations of the non-pathological deterioration of the skin microbiota” means the unsightly and / or unpleasant and / or uncomfortable manifestations due to a non-pathological dysbiosis of the skin microbiota, in particular skin dryness especially dandruff, skin peeling, chapping and / or cracks, roughness of the skin, decrease or loss of resilience of the skin, imperfections of the skin tone, the formation of black dots, the development of odor, hair loss and / or hair loss.

The phytosterols according to the invention can therefore prevent and / or reduce imperfections in the skin tone.

For the purposes of the present invention, the term "preventing and / or reducing blemishes of the skin complexion" means preventing and / or reducing redness or irregularities in the skin and / or homogenizing the complexion so as to render the complexion of the less dull, brighter and more homogeneous skin, and / or decrease the reddish appearance of the skin and / or increase the luminosity of the skin, and / or by giving it a luminous, radiant, healthy and / or nourished appearance , a good-looking effect. Indeed, the radiance of the complexion reflects a state of good health of the skin. Preferably, the imperfections of the skin complexion are chosen from dull complexion, lack of luminosity of the complexion and / or redness. Still preferably, they are chosen from a dull complexion and / or redness.

The measurement of the radiance of the complexion and / or the luminosity of the skin can for example be measured by chromametry or by image analysis. This last in vivo measurement method consists in taking high-resolution photographs in crossed polarized configuration of the face of volunteers taken at 45 ° before and after application of the product tested. On the basis of these digital photographs, an image analysis makes it possible to extract and quantify specific parameters (for example: L *, a *, b *, C, h °) related to color, brightness, homogeneity, and texture of the skin.

By "an aggressive agent of the skin microbiota" is meant climatic variations such as variations in excessive temperatures, in particular hot, cold, air conditioning, wind, UV rays, pollution, pathogens, in particular fungi such as Malassezia. Preferably, these are the aggressive chemical compounds of the microbiota contained in compositions in contact with the skin, topically, medical treatments by the oral or topical route, in particular antibiotics and disinfectants, hygiene products, cosmetics, in particular make-up. Preferably, the aggressive agents of the skin microbiota are not hair treatment agents such as straightening treatments.

Preferably, the aggressive agent of the skin microbiota in particular contained in compositions in contact with the skin is chosen from the group consisting of:

Disodium Lauryl Sulfosuccinate;

MIPA-Laureth Sulfate (Monoisopropanolamine-Laureth Sulfate);

Laureth-4;

Ammonium Lauryl Sulfate;

Sodium Laureth Sulfate,

Sodium Laureth 8-Sulfate,

Sodium Cocoyl Sulfate,

Sodium Myreth Sulfate,

Sodium Cocoyl Sulfate,

Sodium Oleth Sulfate,

Magnesium Laureth Sulfate,

Monoethanolamine Lauryl Sulfate,

Sodium Laureth Sulfate,

Magnesium Laureth 8-Sulfate,

Magnesium Oleth Sulfate,

Disodium Laureth Sulfosuccinate,

TEA-Lauryl Sulfate (Triethanolamine Laurylsulfate)

Sodium Lauryl Sulfate,

Sodium Coco-Sulfate,

Coco-Betaine

Disodium Cocoamphodi acetate

Cocamidopropyl Betaine

Sodium Lauroamphoacetate

Sodium Cocoamphoacetate

Cocamide MEA (Cocamide Monoethanolamine)

Cocamide DEA (Cocamide Diethanolamine)

Cocamide MIPA (Cocamide Monoisopropanolamine)

Lauramide DEA (Lauramide Diethanolamine)

Oleamide DEA (Oleamide Diethanolamine)

Sodium Cocoyl Isethionate

Lauryl Glucoside

Decyl Glucoside

Caprylyl / Capryl Glucoside

Coco-Glucoside

Sodium Cocoyl Glutamate

Disodium Cocoyl Glutamate

Disodium Lauryl Sulfosuccinate and mixtures thereof.

Advantageously, it is Sodium Lauryl Sulfate.

The use of phytosterols according to the invention is advantageously for increasing the tolerance of the skin to aggressive agents of the skin microbiota.

For the purposes of the present invention, the term “cosmetic use” means a non-therapeutic, non-pharmaceutical use of the phytosterols according to the invention, preferably on healthy skin, in particular a healthy scalp, and / or healthy hair, in particular a use intended for all or part of the skin, preferably the scalp and / or an area of skin, preferably scalp called "healthy", in particular to an area of "healthy" skin and / or an area of leather "healthy" hair.

Within the meaning of the present invention, the term “part of the skin preferably of the scalp and / or skin area, preferably of the so-called“ healthy ”scalp, is understood to mean a part of the skin preferably of the scalp and / or an area of skin. , preferably scalp classified as non-pathological by a dermatologist, that is to say one which does not have an infection, inflammation, scar, disease or skin condition such as folliculitis, candidiasis, psoriasis, ichthyosis, pathologies linked to melanogenesis such as vitiligo, eczema, acne, actinic keratosis, carcinoma, melanoma, boil or dermatitis, in particular seborrheic, alopecia or wounds or wounds. In particular, the term “part of the skin”, preferably of the scalp and / or “skin area”, preferably of the so-called “healthy” scalp, is understood to mean a part of the skin preferably of the scalp and / or an area of skin, preferably scalp, described as "normal" by a doctor, particularly a dermatologist, that is to say, made up of "normal" cells, that is to say non-pathological cells, more particularly non-cancerous cells.

Thus, unless otherwise indicated, “normal” skin is understood to mean skin not presenting any pathology.

According to the invention, the phytosterols according to the invention can be used alone in the form of active ingredient or in combination with a fatty alcohol, preferably cetearyl alcohol and / or in a composition intended to be in contact with the skin, such as for example a detergent, hygiene and / or cosmetic composition, preferably intended for topical application.

The term topical application, used here, means applying the phytosterols according to the present invention, optionally in the form of active ingredient and / or composition, on the surface of the skin including the scalp in particular by direct application or by spraying.

The active ingredient and / or the cosmetic compositions containing the phytosterols according to the invention are preferably intended for the cosmetic care and / or treatment of the skin, including the scalp.

In another embodiment, the phytosterols according to the invention can be incorporated into a cosmetic composition further comprising at least one cosmetically acceptable excipient.

For the purposes of the present invention, the term “cosmetically acceptable” excipient means a compound and / or solvent that is topically acceptable, that is to say that does not induce an allergic response on contact with the skin, including the human scalp, not toxic, not unstable, or their equivalents, undue.

For the purposes of the present invention, the term “cosmetic composition” means a non-therapeutic composition, that is to say a composition intended for the prevention and / or care of the skin, including the scalp, called “normal” by a dermatologist, that is to say non-pathological. The term “normal” skin or scalp is understood here to mean healthy skin or scalp as defined above.

In a preferred embodiment of the invention, the phytosterols according to the invention are present in the composition, preferably cosmetic in a content of 0.01 to 10% by weight, preferably from 0.1% to 5% by weight, still advantageously from 0.2% to 3% by weight, more preferably from 0.25% to 1.5% by weight, relative to the total weight of the composition.

In another embodiment, the phytosterols according to the invention may be in the form of a hygiene composition or a maintenance composition, in particular a detergent, further comprising at least one suitable excipient.

The cosmetic composition according to the invention can be in all dosage forms conventionally used for topical application to the skin including the scalp, such as liquid or solid forms or even in the form of pressurized liquid. They can in particular be formulated in the form of an aqueous or oily solution, an aqueous cream or gel or an oily gel, in particular in a jar or in a tube, in particular a shower gel, a shampoo, a conditioner, a milk, a oil, an emulsion, a hydrogel, a microemulsion or a nanoemulsion, in particular oil-in-water or water-in-oil or multiple or silicone, a serum, a lotion, in particular in a glass, plastic or dosing bottle or in aerosol or spray, an ampoule, a liquid or solid soap, a paste, an ointment, a foam, a mask, a lacquer, a patch, an anhydrous product, preferably liquid, pasty or solid, for example in the form of stick in particular in stick or in powders. It can also be a make-up product or a make-up removal product. In particular, the cosmetic composition is chosen from the group consisting of a serum, a lotion, a cream, a shampoo, a conditioner, an oil, a milk, an ointment, a paste, a foam, an emulsion, a hydrogel, a shower gel, mask, hairspray, spray, wax, more preferably it is a cream, serum or lotion.

The compositions according to the invention may contain any suitable solvent and / or any suitable vehicle and / or any suitable excipient, optionally in combination with other compounds of interest.

Therefore, for these compositions, the excipient contains for example at least one compound chosen from the group consisting of preservatives, emollients, emulsifiers, surfactants, moisturizers, thickeners, conditioners, matting agents, stabilizers, antioxidants, texture agents, shine agents, film-forming agents, solubilizers, pigments, dyes, perfumes and sunscreens. These excipients are preferably chosen from the group consisting of amino acids and their derivatives, polyglycerols, esters, polymers and cellulose derivatives, Lanolin derivatives, phospholipids, lactoferrins, lactoperoxidases, stabilizers based on sucrose, vitamins E and its derivatives, natural and synthetic waxes, vegetable oils, triglycerides, unsaponifiables, other phytosterols, vegetable esters, silicones and its derivatives, protein hydrolysates, Jojoba oil and its derivatives, lipo / water-soluble esters, betaines, aminoxides, plant extracts sucrose esters, titanium dioxides, glycines, and parabens, and more preferably from the group consisting of butylene glycol, steareth-2, steareth-21, glycol-15 stearyl ether, cetearyl alcohol, phenoxyethanol, methylparaben, ethylparaben, propylpa raben, butylparaben, butylene glycol, natural tocopherols, glycerin, dihydroxycetyl sodium phosphate, isopropyl hydroxyketyl ether, glycol stearate, triisononanoin, octyl cocoate, polyacrylamide, isoparaffin, laureth-7 , a carbomer, propylene glycol, glycerol, bisabolol, dimethicone, sodium hydroxide, PEG 30dipolyhydroxysterate, capric / caprylic triglycerides, cetearyl octanoate, dibutyl adipate, grapeseed oil, l jojoba oil, magnesium sulfate, EDTA, cyclomethicone, xanthan gum, citric acid, sodium lauryl sulfate, waxes and mineral oils, isostearyl isostearate, propylene glycol dipelargonate , propylene glycol isostearate, PEG 8, Beeswax, hydrogenated palm heart oil glycerides, hydrogenated palm oil glycerides, lanolin oil, sesame oil, cetyl la ctate, lanolin alcohol, castor oil, titanium dioxide, lactose, sucrose, low density polyethylene, salty isotonic solution.

Many cosmetically active ingredients are known to those skilled in the art for improving the health and / or the physical appearance of the skin. Those skilled in the art know how to formulate cosmetic or dermatological compositions to obtain the best effects. On the other hand, the compounds described in the present invention can have a synergistic effect when they are combined with one another. These combinations are also covered by the present invention. The CTFA Cosmetic Ingredient Handbook, Second Edition (1992) describes various cosmetic and pharmaceutical ingredients commonly used in the cosmetic and pharmaceutical industry, which are in particular suitable for topical use. Examples of these classes of ingredients include, without being limited to the following compounds: abrasive, absorbents, compound for aesthetic purposes such as perfumes, pigments, dyes, essential oils, astringents, etc. (for example: clove oil, menthol, camphor, eucalyptus oil, eugenol, menthyl lactate, witch hazel distillate), anti-acne agents, anti-flocculants, defoamers, antimicrobial agents (for example: iodopropyl butylcarbamate), antioxidants, binders, biological additives, buffering agents, bulking agents, chelating agents, additives, biocidal agents, denaturants, thickeners, and vitamins, and derivatives or equivalents thereof, film-forming materials, polymers, clouding agents, pH adjusters, reducing agents, depigmenting or lightening agents (e.g. hydroquinone, kojic acid, ascorbic acid, magnesium ascorbyl phosphate, ascorbyl glucosamine), conditioning agents (for example: humectants).

In a particularly advantageous manner, the phytosterols according to the invention can be used, optionally in a cosmetic or pharmaceutical composition, preferably dermatological, preferably those previously described, as the only active agent, in particular as the only active agent which protects and / or restores the skin microbiota or in combination with one or more other active agents having complementary beneficial effects on the microbiota:

- the antibacterial agents described in patent application FR2863893, and in particular an extract of Peumus boldus, such an extract being especially marketed by the Applicant under the name Betapur ™ and / or a local antibiotic agent, in particular erythromycin and / or clindamycin phosphate.

agents balancing the microbial flora such as a mixture of pullulan, sodium alginate and sodium hyaluronate marketed by the Applicant under the name PatcH20 ™ in particular associated with serine, trehalose and urea and described in patent application WO2014027163A2, and / or an extract of Saccharomyces cerevisiae to increase the commensal cutaneous and / or mucosal microbial flora sold by the Applicant under the name Relipidium ™.

- comedolytic agent: retinoic acid and one of its derivatives such as isotretinoin, adapalene and / or 13cis retinoic acid and benzoyl peroxide;

- hydrating agents: one or more agents promoting hydration such as a polysaccharide extracted from Cassia angustifolia seeds marketed under the name of Hyalurosmooth ™ by the applicant, or an agent chosen from one of the combinations containing pullulan, hyaluronate sodium and sodium gin alginate marketed under the name of PatcH2O ™ by the applicant or one or more of the compounds of the natural moisturizing factor (Natural Moisturizing Factor) or a natural extract of honey marketed by the applicant under the name of Melhydran ™ and / or a compound of the glucosyl glycerides family, in particular hexosyl glyceride, an extract of Litchi chinensis pericarp under the name Litchiderm ™ by the applicant;

The cosmetic compositions containing the phytosterols according to the invention may contain the conventional active ingredients of the cosmetic compositions especially chosen from:

an agent stimulating the synthesis of fibronectin, in particular an extract of corn, such an extract being in particular marketed by the Applicant under the name Deliner ™ 'an agent for the protection of the fibroblast growth factor (EGE2) of the extracellular matrix against its degradation and / or its denaturation, in particular an A'Hibiscus Abelmoscus extract as described in the patent application in the name of the Applicant filed under number ER0654316 and / or a fibroblast growth stimulating agent, for example a fermented soy extract containing peptides, known under the name of Phytokine ™ marketed by the Applicant and also described in patent application EPI 119344 B1 (Laboratoires Expanscience), and preferably a combination of these two extracts;

an agent stimulating the synthesis of laminin, in particular a malt extract modified by biotechnology, such an extract being in particular marketed by the Applicant under the name Basaline ™; and a lipid synthesis stimulating agent such as a potato extract (solanum tuberosum) modified by biotechnology marketed by the Applicant under the name Lipidessence ™;

- an agent stimulating the expression and / or the activity of Hyaluronane synthase 2 (HAS2) such as the plant extracts described in patent application ER2 893 252 A1 and in particular an aqueous extract of Galangal (Alpinia galanga);

- an agent stimulating the synthesis of lysyl oxidase like (LOXL) such as those described in patent application ER2855968, and in particular a dill extract;

- one or more anti-pollution agents such as an extract of ft Argania spinosa leaves marketed under the name of Arganyl ™ or an extract of seeds of Moringa oleifera marketed under the name of Purisoft ™ by the applicant or even a root extract A'Eperua falcata marketed under the name of Eperuline ™

- an agent stimulating the synthesis of intracellular ATP, in particular an extract of alga Laminaria digitata;

- an agent with global anti-aging action, in particular anti-pigmentary tasks in particular niacinamide or vitamin B3;

and any of their mixtures.

According to the present invention, the use of the phytosterols according to the invention is particularly advantageous in that it allows a complete, effective and lasting action on any type of skin including scalp, in particular skin whose microbiota has been attacked by aggressive agents.

Preferably, the phytosterols according to the invention, preferably in the form of a cosmetic composition according to the invention are applied to at least one area of the body where there are uncomfortable and / or unsightly and / or unpleasant manifestations of alteration of the microbiota, this or these zones being preferably a surface of the body chosen from the skin of the face, including the forehead, cheeks, nose, temples, T zone (forehead, nose and chin), chin, leather scalp, neck, back, shoulders, forearms, thorax, hands, and / or bust, especially legs, feet, armpits, hands, neck, cleavage, stomach , arms, thighs, hips, buttocks, waist, crotch, groin, torso, back, face and / or scalp, especially maceration areas such as the seat of the infant, plaice areas such as armpits, back of elbows, back re knees, the buttocks, the crotch, groin, neck, and / or around the lips.

The present invention also relates to a cosmetic care process comprising the topical application on at least one skin area, advantageously of the scalp, of the phytosterols according to the invention, preferably on at least one surface chosen from the skin of the skin. face, including forehead, cheeks, nose, temples, T-zone (forehead, nose and chin), chin, scalp, neck, back, shoulders, forearm, chest, hands, and / or bust, especially legs, feet, armpits, hands, neck, cleavage, stomach, arms, thighs, hips, buttocks, waist, crotch , groin, torso, back, face and / or scalp, especially maceration areas such as infant seat, plaice areas such as armpits, back of elbows, back knees, buttocks, crotch, groin, neck, and / or around the lips to protect and / or restore er the skin microbiota and / or to prevent and / or reduce unsightly and / or uncomfortable and / or unpleasant manifestations of the alteration of the skin microbiota.

Advantageously, the phytosterols according to the invention, preferably in the form of a composition intended for topical application, preferably cosmetic according to the invention, are used in regular topical application and preferably at least once a day, advantageously twice per day, for at least 7 days. Preferably, the cosmetic composition is applied to the skin without rinsing by massaging. Preferably, the application is concomitant or sequential with that of an aggressive agent of the skin microbiota.

Advantageously, the invention also relates to a cosmetic treatment method for protecting and / or restoring the skin microbiota of an individual who needs it / who wishes it, comprising the steps:

a) The identification on the individual of an area of skin whose skin microbiota must be protected and / or is altered and / or having unsightly and / or unpleasant and / or uncomfortable manifestations linked to the alteration of the skin microbiota , and

b) Topical application to this skin area of a cosmetic composition containing the phytosterols according to the invention in an amount effective to prevent and / or restore the skin microbiota on this skin area, namely advantageously in a phytosterol content between 0.01% and 10% by weight, preferably from 0.1% to 5% by weight, still advantageously from 0.2% to 3% by weight, still preferably from 0.25% to 1.5% by weight weight, relative to the total weight of the composition.

The phytosterols according to the invention can also be used for the treatment and / or prevention and / or reduction of the occurrence of a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of acne, psoriasis, bacterial and / or fungal infections, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, erythyma erysipelas, yeast infections such as candidiasis or dermatophytosis such as ringworm and / or scabies and / or acne scars and / or any combination thereof and / or in the treatment and / or prevention of superinfection of wounds and / or in the prevention of stains.

Preferably, the phytosterols according to the invention, preferably in the form of a pharmaceutical composition according to the invention, are applied to at least one area of the body having a pathology due to an altered skin microbiota, in particular to at least one area of the body presenting a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of psoriasis, acne and / or a bacterial and / or fungal infection, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, ecthyma erysipelas, mycoses such as candidiasis or dermatophytoses such as ringworm and / or scabies pigment and / or acne scars, this or these zones preferably being a surface of the body chosen from the skin of the face, including the forehead, the cheeks, the nose, the temples, the T zone (forehead, nose and chin), and / or the chin, the scalp, neck, back, shoulders, forearms, chest, hands, and / or bust, especially legs, feet, armpits, hands, neck, décolleté, belly, arms, thighs, hips, buttocks, waist, crotch, groin, torso, back, face and / or scalp, especially maceration areas such as the seat of the infant, the areas of plaice such as the armpits, the back of the elbows, the back of the knees, the buttocks, the crotch, the groin, the neck, and / or around the lips.

In a preferred embodiment of the invention, the phytosterols according to the invention are in the form of a pharmaceutical composition further comprising a pharmaceutically acceptable excipient and are present in the pharmaceutical composition in a content of between 0.01% to 10% by weight, preferably from 0.1% to 5% by weight, still advantageously from 0.2% to 3% by weight, still preferably from 0.25% and 1.5% by weight, relative to the total weight of the composition.

Such pharmaceutical compositions can in particular be in the form of medical devices of the dressing type, in particular in combination with aggressive agents of the skin microbiota chosen from antiseptic agents, disinfecting agents and mixtures thereof.

Other objects, characteristics and advantages of the invention will become apparent to those skilled in the art after reading the explanatory description which refers to examples which are given only by way of illustration and which cannot be in no way limit the scope of the invention.

The examples form an integral part of the present invention and any characteristic which appears new compared to any prior state of the art from the description taken as a whole, including the examples, forms an integral part of the invention in its function and in its generality.

Thus, each example is general in scope.

On the other hand, in the examples, all the percentages are given by weight, unless otherwise indicated, the temperature is expressed in degrees Celsius unless otherwise indicated, and the pressure is atmospheric pressure, unless otherwise indicated.

EXAMPLES

EXAMPLE 1: method for preparing phytosterols according to the invention la) Preparation of phytosterols:

Rapeseed oil (Brassica campestris) is obtained by conventional methods and is commercially available - according to a production method it can be obtained by grinding the seeds and then cold extraction. After removing impurities by filtration, the seed oil is transesterified and distilled to obtain a phytosterol concentrate. Crystals of these phytosterols are obtained by cooling, and purified by successive stages of filtration and washing with solvents. The washing solvents are removed by heating under vacuum, and the molten phytosterols are obtained in the form of powder by passage over a priller jet, to obtain purified phytosterols (> 95%).

lb) Preparation of phytosterols mixed with a fatty alcohol according to the invention

The purified phytosterols obtained in Example 1a) are mixed with cetearyl alcohol (INCI name: Cetearyl Alcohol) with a 1: 1 ratio (weight: weight). The mixture is then heated above the melting point and atomized under cold air flow (prilling technology) to obtain a powder.

EXAMPLE 2 Evaluation of the Improvement Effect of the Skin Microbiota by a Formulation Containing the Phytosterols According to the Invention in the Presence of an Aggressive Agent of the Skin Microbiota.

Protocol:

The test was conducted on 29 female volunteers on whom an aqueous solution of SLS (sodium lauryl sulfate) at a concentration of 0.5% (w / w) relative to the total weight of the solution was applied to the skin at using a patch. After 24 hours of application, the patch is removed and the skin is left to rest without applying any product. After 24 hours after removing the patch, the oily composition described in Example 3a) containing the phytosterols according to the invention as obtained in Example 1b) at 2% by weight relative to the total weight of the composition is applied once a day for 7 days. The skin microbiota is taken by swab, before the application of SLS, 24 hours after removal of the patch containing SLS, and after the period of application of the formulation containing the product of the invention.

Microbial DNA is extracted by a double mechanical and chemical action, quantified by fluorescence and then analyzed by metasequencing of a fragment of 16S ribosomal RNA. The primers used to amplify the hypervariable region of LARN16S are: 5'CCTACGGGNGGCWGCAG'3 (SEQ ID No. 1) and 5'GACTACHVGGGTATCTAATCC'3 (SEQ ID No. 2).

The results obtained are recorded in Table I in the form of an average of relative abundances and of deviation from the mean (SEM) calculated by the method of least squares.

Kind Average (SEM) before patch application ("Reference") After application 24H patch ("post patch ”) P expressed vs reference 7 days application forn 3A) P expressed vs Po after tiulation st patch Resident commensal and mutual skin bacteria Brevibacterium 0.151 (0.184) 0.172 (0.190) P = 0.921 0.788 (0.193) P = 0.0195 Corynebacterium 2.551 (0.825) 1.343 (0.832) P = 0.019 2,532 (0.835) P = 0.019 Kocuria 1.669 (0.386) 1.125 (0.390) P = 0.0575 1.293 (0.392) Micrococcus 2.849 (0.726) 1.487 (0.732) P = 0.003 2.468 (0.735) P = 0.03 Roseomonas 0.235 (0.086) 0.169 (0.087) 0.247 (0.087) Brevundimonas 0.151 (0.044) 0.065 (0.045) 0.089 (0.046) Enhydrobacter 3.731 (0.813) 1,988 (0.833) P = 0.0839 2,874 (0,842) P = 0.2745 Paracoccus 4.028 (0.852) 2.408 (0.862) P = 0.017 3.53 (0.868) P = 0.090 Bacteria pathogenic skin opportunities Pantoea 0.009 (1.186) 4.072 (1.235) P = 0.033 0.437 (1.259) P = 0.040 Pseudomonas 0.167 (0.144) 0.518 (0.149) P = 0.10 0.199 (0.151) P = 0.10

P is obtained by the One way Annova test.

The composition containing the phytosterols of the invention made it possible to compensate for the disturbances induced by the aggressive agents of the microbiota by restoring the initial content of the beneficial commensal microorganisms which was reduced by the detergent SLS. The composition containing the phytosterols according to the invention has reinduced the abundance of beneficial commensal microorganisms from the group of actinobacteria and proteobacteria, more specifically the abundance of commensals of the genera micrococcus, Kocuria, Corynebacterium, and Brevibacterium 10 belonging to the families micrococcaceae, corynebacteriaceae , brevibacteriaceae. Likewise, it has reinduced the abundance of beneficial commensal microorganisms from the proteobacteria group with the genera Roseomonas, Brevundimonas

Enhydrobacter and paracoccus belonging respectively to the families Acetobacteracea, Caulobacteraceae, Moraxellaceae, and Rhodobacteriaceae.

The composition containing the phytosterols according to the invention reduced the abundance of opportunistic pathogenic microorganisms such as the genera Pantoea and 5 pseudomonas both belonging to the group of proteobacteria and to the families of Enterobacteriaceae and pseudomonaceae.

In conclusion, the phytosterols according to the invention restored the microbiota which was disturbed by the use of an aggressive agent of the microbiota here a detergent agent.

EXAMPLE 3 Cosmetic Composition According to the Invention

The phytosterols used are those obtained in Example 1b) (after mixing with cetearyl alcohol) in powder form.

EXAMPLE 3A Oil for Skin Care

Denomination Quantity (% by total weight) Vegetable oil 18.00 Caprylic / capric triglyceride 40.00 Coconut caprylate 40.00 Phytosterols according to the invention 2.00

Example 3B) Body Balm

Phase Denomination Quantity (% by total weight) AT Cetaryl glucoside / cetaryl alcohol 6.00 AT Glutamate stearoyl sodium 0.50 AT Pentaerythrityl distearate 3.00 AT Glyceryl stearate 2.00 AT Carbonate dicaprylyl 4.00 AT Ether Dicaprylyl 2.00 AT Butyrospermum parkii butter 4.00 AT Elaieis guineensis oil 1.00 AT Shorea stenoptera grain butter 2.00 AT Sodium polyacrylate 0.75

AT Polydimethylsiloxane 1.00 AT Phytosterols according to the invention 1.00 AT Octyldodecanol, Irvingia gabonensis, hydrogenated coconut glycerides 0.50 B Water 66.25 B Glycerin 5.00 VS Cyperus esculentus root oil 1.00 VS Perfume and preservatives QSP qs

The cream is prepared by the usual methods in the field well known to those skilled in the art, by mixing the 3 phases and adjusting the composition to a pH of 6.3.

EXAMPLE 30 Lip Stick

Phase Denomination Quantity (% by total weight) AT Oil blend (INCI: Olus oil), hydrogenated vegetable oil, Candelilla cera, vegetable oil, Euphorbia cerifera wax (Cegesoft® VP) 37.75 AT Hydrogenated vegetable oil 5.00 AT Hydrogenated castor oil 5.00 AT Cetyl palmitate 7.00 AT Myristyl myristate 7.00 AT Behenyle alcohol 5.00 AT Phytosterols according to the invention 3.00 AT Butyrospermum parkii butter 3.00 AT Mixture of oil (INCI: Olus oil) and vegetable oil (Cegesoft ® PS 6) 12.00 AT Passifkora incamata seed oil 5.00 AT Talc 5.00 B Dicaprylyl carbonate, stearalkonium hectorite, propylene carbonate 2.00

VS Tocopherol 1.00 vs Stone oil <1 'Argania spinosa 2.00 vs Tetradibutyl pentaerithrityl hydroxyhydrocinnamate 0.05 D Perfume 0.20

The stick is prepared by the usual methods in the field well known to those skilled in the art, by heating phase A at the temperature of 80 to 85 ° C with moderate stirring. When it is melted and homogeneous, phase B is added by mixing and maintaining the temperature. The whole is then stirred for 10 min at 80 ° C. The compounds of phases C and D are added and the whole is added and hot-packed.

Claims (21)

1. Non-therapeutic cosmetic use on healthy skin and / or healthy hair of phytosterols to protect and / or restore the cutaneous microbiota and / or to prevent and / or reduce unsightly and / or unpleasant and / or uncomfortable manifestations of alteration of the skin microbiota. 2. Use according to claim 1, characterized in that the unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota are skin dryness, in particular dandruff, skin peeling, cracks and / or cracks, roughness of the skin, decrease or loss of the resilience of the skin, imperfections of the skin complexion, the formation of black dots, the development of odor, hair loss and / or hair loss. 3. Use according to any one of claims 1 or 2, characterized in that the phytosterols are extracted from rapeseed oil. 4. Use according to any one of claims 1 to 3, characterized in that the phytosterols contain: - from 30 to 70%, preferably from 40 to 60% of beta-sitosterol, by weight relative to the total weight of phytosterols - from 25 to 60% of campesterol, preferably from 25% to 40%, by weight relative to the total weight of phytosterols from 5 to 25% of brassicasterol, preferably from 7 to 18%, by weight relative to the total weight of the phytosterols. 5. Use according to any one of claims 1 to 4, characterized in that the phytosterols are mixed with a fatty alcohol, advantageously the phytosterols are mixed with cetearyl alcohol. 02-14-19 FR1858219 6. Use according to claim 5, characterized in that the weight ratio of the fatty alcohol mixture is between 40/60 and 60/40, preferably between 45/55 and 55/45, even more preferably it is 50/50. 7. Use according to any one of claims 1 to 6, characterized in that the phytosterols are in the form of a cosmetic or hygienic composition, preferably a cosmetic, further comprising a cosmetically acceptable excipient. 8. Use according to claim 7, characterized in that the cosmetic composition is intended for topical application to the skin, in particular the scalp. 9. Use according to any one of claims 7 or 8, characterized in that the phytosterols are present in the composition in a content of 0.01% to 10% by weight, preferably from 0.1% to 5% by weight , still advantageously from 0.2% to 3% by weight, more preferably from 0.25% to 1.5% by weight, relative to the total weight of the composition. 10. Use according to any one of claims 1 to 9 for protecting the skin microbiota from the effects of an aggressive agent of the skin microbiota. 11. Use according to claim 10, characterized in that the aggressive agent is chosen from the group consisting of Disodium Lauryl Sulfosuccinate, Monoisopropanolamine-Laureth Sulfate, Laureth-4, ammonium Lauryl Sulfate, Sodium Laureth Sulfate, Sodium Laureth 8-Sulfate, Sodium Cocoyl Sulfate, Sodium Myreth Sulfate, Sodium Cocoyl Sulfate, Sodium Oleth Sulfate, Magnesium Laureth Sulfate, Monoethanolamine Lauryl Sulfate, Sodium Laureth Sulfate, Magnesium Laureth 8-Sulfate, Magnesium Oleth Sulfate, Disodium Laureth Sulfosuccinate, Triethanolamine Laurylsulfate, Sodium Lauryl Sulfate, Sodium CocoSulfate, Coco-Betaine, Disodium Cocoamphodiacetate, Cocamidopropyl Bétaine, Sodium Lauroamphoacetate, Sodium Cocoamphoate 02-14-19 FR1858219 Cocamide Monoethanolamine, Cocamid Diethanolamine, Cocamide Monoisopropanolamine, Lauramide Diethanolamine, Oleamide Diethanolamine, Sodium Cocoyl Isethionate, Lauryl Glucoside, Decyl Glucoside, Caprylyl / Capryl Glucoside, Coco-Glucoside, Sodium Disodium Cocoyl Glutamate Disodium Lauryl Sulfosuccinate and their mixtures, advantageously it is Sodium Lauryl Sulfate. 12. Use according to any one of claims 10 or 11, characterized in that the phytosterols are applied concomitantly or sequentially with the aggressive agent of the skin microbiota. 13. Use according to any one of claims 10 to 12, characterized in that the phytosterols and the aggressive agent of the skin microbiota are contained in the same composition. 14. Use according to any one of claims 7 to 13, characterized in that the cosmetic composition is chosen from the group consisting of a serum, a lotion, a cream, a shampoo, a conditioner, an oil, a milk , an ointment, a paste, a foam, an emulsion, a hydrogel, a shower gel, a mask, a lacquer, a spray, a wax, advantageously it is a cream, a serum or a lotion. 15. Use according to any one of claims 1 to 14 as a skin prebiotic and / or to increase the tolerance of the skin to aggressive agents of the skin microbiota. 16. Cosmetic care method characterized in that it comprises the topical application to at least one zone of healthy skin, advantageously of the scalp, of phytosterols to protect and / or restore the cutaneous microbiota and / or to prevent and / or reduce the unsightly and / or unpleasant and / or uncomfortable manifestations of the alteration of the skin microbiota. 02-14-19 FR1858219 17. Cosmetic care method according to claim 16, characterized in that the phytosterols are as defined in any one of claims 3 to 7, 9, 13 and 14. 18. Cosmetic care method according to any one of claims 16 or 17, characterized in that this skin area is a surface of the body chosen from the skin of the face, including the forehead, the cheeks, the nose, the temples, T-zone (forehead, nose and chin), and / or chin, scalp, neck, back, shoulders, forearms, chest, hands, and / or bust, especially legs, feet, armpits, hands, neck, cleavage, stomach, arms, thighs, hips, buttocks, waist, crotch, groin, torso, back, face and / or scalp, in particular maceration areas such as the infant seat, plaice areas such as the armpits, the back of the elbows, the back of the knees, the buttocks, the crotch, the groin, neck, and / or around the lips. 19. Phytosterols for their use in the treatment and / or prevention and / or reduction of the occurrence of a pathology associated with an alteration of the skin microbiota, in particular chosen from the group consisting of acne, psoriasis and infections bacterial and / or fungal, ulcers, herpes, boils, folliculitis, abscesses, sycosis, impetigo, erythyma erysipelas, fungal infections such as candidiasis or dermatophytoses such as ringworm and / or scabies and / or acne scars and their combinations and / or in the treatment and / or prevention of superinfection of wounds and / or in the prevention of blemishes, 20. Phytosterols for their use according to claim 19, characterized in that the phytosterols are as defined according to one of claims 3 to 6. 21. Phytosterols for their use according to any one of claims 19 or 20, characterized in that the phytosterols are in the form of a pharmaceutical composition further comprising a pharmaceutically acceptable excipient and are present in said composition 02-14-19 FR1858219 pharmaceutical in a content ranging from 0.01% to 10% by weight, preferably from 0.1% to 5% by weight, still advantageously from 0.2% to 3% by weight, still preferably from 0.25% to 1.5% by weight, relative to the total weight of the composition.