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EA202091349A1 - Способы культивирования клеток - Google Patents

Способы культивирования клеток

Info

Publication number
EA202091349A1
EA202091349A1 EA202091349A EA202091349A EA202091349A1 EA 202091349 A1 EA202091349 A1 EA 202091349A1 EA 202091349 A EA202091349 A EA 202091349A EA 202091349 A EA202091349 A EA 202091349A EA 202091349 A1 EA202091349 A1 EA 202091349A1
Authority
EA
Eurasian Patent Office
Prior art keywords
methods
cell culture
cell cultivation
culture medium
heterogeneity
Prior art date
Application number
EA202091349A
Other languages
English (en)
Inventor
Валентин Шеваллье
Надин Кочановски
Летисия Мальфетт
Венсен Адольф Кароль Кол
Original Assignee
Юсб Биофарма Срл
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Юсб Биофарма Срл filed Critical Юсб Биофарма Срл
Publication of EA202091349A1 publication Critical patent/EA202091349A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/0018—Culture media for cell or tissue culture
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/46—Hybrid immunoglobulins
    • C07K16/461—Igs containing Ig-regions, -domains or -residues form different species
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/565—Complementarity determining region [CDR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Cell Biology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Настоящее изобретение относится к способам снижения гетерогенности популяции рекомбинантных белков, продуцируемых в клеточной культуре, где указанные способы включают культивирование клеток-хозяев, продуцирующих рекомбинантный белок в среде для культивирования клеток, где среда для культивирования клеток содержит один или более аналогов цистеина/цистина.
EA202091349A 2017-12-01 2018-11-29 Способы культивирования клеток EA202091349A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP17204978.5A EP3492582A1 (en) 2017-12-01 2017-12-01 Cell culture methods
PCT/EP2018/083010 WO2019106091A1 (en) 2017-12-01 2018-11-29 Cell culture methods

Publications (1)

Publication Number Publication Date
EA202091349A1 true EA202091349A1 (ru) 2020-08-20

Family

ID=60629439

Family Applications (1)

Application Number Title Priority Date Filing Date
EA202091349A EA202091349A1 (ru) 2017-12-01 2018-11-29 Способы культивирования клеток

Country Status (14)

Country Link
US (1) US11859203B2 (ru)
EP (2) EP3492582A1 (ru)
JP (1) JP7422072B2 (ru)
KR (1) KR20200092348A (ru)
CN (1) CN111417715B (ru)
BR (1) BR112020010473A2 (ru)
CA (1) CA3083680A1 (ru)
EA (1) EA202091349A1 (ru)
ES (1) ES2963653T3 (ru)
HR (1) HRP20231175T1 (ru)
HU (1) HUE063812T2 (ru)
PL (1) PL3717631T3 (ru)
SG (1) SG11202004935UA (ru)
WO (1) WO2019106091A1 (ru)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3492582A1 (en) 2017-12-01 2019-06-05 UCB Biopharma SPRL Cell culture methods
CN112391350A (zh) * 2020-10-30 2021-02-23 北京双鹭药业股份有限公司 高效表达抗egfr单克隆抗体细胞的筛选培养方法
GB202105424D0 (en) 2021-04-16 2021-06-02 UCB Biopharma SRL Cell culture processes
EP4166651A1 (en) 2021-10-18 2023-04-19 Evonik Operations GmbH Methods and composition to increase cellular glutathione level
WO2023067626A1 (en) * 2021-10-20 2023-04-27 Dr. Reddy's Laboratories Limited A cell culture process to produce an antibody composition
CN118742652A (zh) * 2022-02-21 2024-10-01 中外制药株式会社 灌流培养方法
WO2024024720A1 (ja) * 2022-07-25 2024-02-01 味の素株式会社 細胞培養用の培地

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4927762A (en) 1986-04-01 1990-05-22 Cell Enterprises, Inc. Cell culture medium with antioxidant
EP2221361A3 (en) 1996-08-30 2011-02-09 Life Technologies Corporation Method for producing a polypeptide in vitro in mammalian cells in a protein-free and serum-free culture medium
EP1120407B1 (en) * 1998-10-09 2013-02-20 Ajinomoto Co., Inc. Cystine derivatives
KR20050070512A (ko) 2003-12-30 2005-07-07 삼성정밀화학 주식회사 재조합 단백질의 생산성을 증가시키는 무혈청 배지 및동물세포 배양방법
EP2500415A1 (en) 2006-09-13 2012-09-19 Abbott Laboratories Cell culture improvements
CA2842966A1 (en) * 2006-09-13 2008-03-20 Abbvie Inc. Cell culture improvements
AU2011246502B2 (en) 2010-04-26 2014-08-28 Novartis Ag Improved cell cultivation process
WO2013158275A1 (en) 2012-04-20 2013-10-24 Abbvie Inc. Cell culture methods to reduce acidic species
US20130281355A1 (en) 2012-04-24 2013-10-24 Genentech, Inc. Cell culture compositions and methods for polypeptide production
GB201208367D0 (en) 2012-05-14 2012-06-27 Ucb Pharma Sa Biological product
CN106715707A (zh) 2014-06-18 2017-05-24 免疫医疗公司 包含n‑乙酰半胱氨酸的细胞培养方法和培养基
EP3448871B1 (en) * 2016-04-28 2023-10-11 Merck Patent GmbH Method for reducing the trisulfide level in proteins
EP3492582A1 (en) 2017-12-01 2019-06-05 UCB Biopharma SPRL Cell culture methods

Also Published As

Publication number Publication date
EP3717631A1 (en) 2020-10-07
CA3083680A1 (en) 2019-06-06
US11859203B2 (en) 2024-01-02
WO2019106091A1 (en) 2019-06-06
JP2021503938A (ja) 2021-02-15
EP3717631C0 (en) 2023-08-30
PL3717631T3 (pl) 2024-03-04
ES2963653T3 (es) 2024-04-01
KR20200092348A (ko) 2020-08-03
SG11202004935UA (en) 2020-06-29
EP3717631B1 (en) 2023-08-30
CN111417715A (zh) 2020-07-14
BR112020010473A2 (pt) 2020-11-24
EP3492582A1 (en) 2019-06-05
JP7422072B2 (ja) 2024-01-25
HUE063812T2 (hu) 2024-02-28
CN111417715B (zh) 2024-08-27
HRP20231175T1 (hr) 2024-01-19
US20200308536A1 (en) 2020-10-01

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