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BR112017007770A2 - cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc. - Google Patents

cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.

Info

Publication number
BR112017007770A2
BR112017007770A2 BR112017007770A BR112017007770A BR112017007770A2 BR 112017007770 A2 BR112017007770 A2 BR 112017007770A2 BR 112017007770 A BR112017007770 A BR 112017007770A BR 112017007770 A BR112017007770 A BR 112017007770A BR 112017007770 A2 BR112017007770 A2 BR 112017007770A2
Authority
BR
Brazil
Prior art keywords
ips cells
human ips
hipscs
hipsc
population
Prior art date
Application number
BR112017007770A
Other languages
English (en)
Inventor
M Valenzuela David
KUNO Junko
Auerbach Wojtek
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BR112017007770A2 publication Critical patent/BR112017007770A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0696—Artificially induced pluripotent stem cells, e.g. iPS
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/32—Amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/38—Vitamins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/30—Organic components
    • C12N2500/44—Thiols, e.g. mercaptoethanol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/60—Buffer, e.g. pH regulation, osmotic pressure
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
    • C12N2501/72—Transferases [EC 2.]
    • C12N2501/727—Kinases (EC 2.7.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Developmental Biology & Embryology (AREA)
  • Microbiology (AREA)
  • Transplantation (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

a presente invenção refere-se aos métodos e composições que são fornecidos para gerar ou manter as células humanas ips em cultura. os métodos incluem o uso de um meio de baixa osmolalidade para fazer células humanas ips, ou usar um meio de baixa osmolalidade para manter as células humanas ips. métodos para fazer a modificação genética direcionada às células humanas ips cultivadas no meio de baixa osmolalidade também estão incluídos. as composições incluem células humanas ips cultivadas e mantidas usando o meio de baixa osmolalidade aqui definido.
BR112017007770A 2014-10-15 2015-10-15 cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc. BR112017007770A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201462064384P 2014-10-15 2014-10-15
PCT/US2015/055776 WO2016061374A1 (en) 2014-10-15 2015-10-15 Methods and compositions for generating or maintaining pluripotent cells

Publications (1)

Publication Number Publication Date
BR112017007770A2 true BR112017007770A2 (pt) 2018-01-16

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
BR112017007770A BR112017007770A2 (pt) 2014-10-15 2015-10-15 cultura in vitro, população de hipscs, método para modificar um locus-alvo genômico, e, hipsc.

Country Status (18)

Country Link
US (2) US10428310B2 (pt)
EP (2) EP3207124B1 (pt)
JP (1) JP2017536811A (pt)
KR (1) KR20170070136A (pt)
CN (1) CN107002042A (pt)
AU (1) AU2015332451A1 (pt)
BR (1) BR112017007770A2 (pt)
CA (1) CA2963315A1 (pt)
CY (1) CY1122179T1 (pt)
DK (1) DK3207124T3 (pt)
ES (1) ES2741387T3 (pt)
IL (1) IL251576A0 (pt)
MX (1) MX2017004890A (pt)
PL (1) PL3207124T3 (pt)
PT (1) PT3207124T (pt)
RU (1) RU2706965C2 (pt)
SG (2) SG10201913797YA (pt)
WO (1) WO2016061374A1 (pt)

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US20160108369A1 (en) 2016-04-21
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RU2706965C2 (ru) 2019-11-21
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JP2017536811A (ja) 2017-12-14
US10428310B2 (en) 2019-10-01
MX2017004890A (es) 2018-02-12
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US20190382730A1 (en) 2019-12-19

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