CN214991585U

CN214991585U - Device for preparing and quantitatively subpackaging anaerobic microorganism culture medium

Info

Publication number: CN214991585U
Application number: CN202121594170.9U
Authority: CN
Inventors: 陈丹丹; 刘昌庚; 程宽; 郭焱; 牛强
Original assignee: Panzhihua University
Current assignee: Panzhihua University
Priority date: 2021-07-12
Filing date: 2021-07-12
Publication date: 2021-12-03
Anticipated expiration: 2031-07-12

Abstract

The utility model discloses a device of preparation and ration partial shipment anaerobic microorganisms culture medium belongs to experimental facilities technical field. This device includes anaerobic bottle (2), agitator (3), ration system and split charging bottle (5), and agitator (3) are used for anaerobic bottle (2) bottle internal stirring, and the ration system is established between split charging bottle (5) and anaerobic bottle (2) to communicate with split charging bottle (5) and anaerobic bottle (2) respectively. This device can be with adding the required other reaction substrate misce benes of reaction system and realize the purpose of preparation culture medium through agitator (3), and quantitative system can carry out quantitative partial shipment with the culture medium simultaneously for the purpose of preparation and partial shipment is realized simultaneously to this device. The problem of current anaerobic bottle be the ration system, cause the measurement difficulty, the operation is complicated, awkward is solved.

Description

Device for preparing and quantitatively subpackaging anaerobic microorganism culture medium

Technical Field

The utility model discloses a device of preparation and ration partial shipment anaerobic microorganisms culture medium belongs to experimental facilities technical field.

Background

At present, the preparation method and equipment of the laboratory anaerobic microorganism culture medium have no unified standard, which hinders the scientific research of anaerobic microorganisms to a certain extent and affects the comparability of the same-row data. Widdel-flash is an anaerobic bottle which is used more at present, but the Widdel-flash lacks a quantitative system, is complex to operate and high in price, and brings difficulty to popularization and use. In response to these problems, there is a need for an apparatus and method of use for preparing and dosing anaerobic microorganism culture media.

SUMMERY OF THE UTILITY MODEL

The utility model aims to solve the technical problem that the existing anaerobic bottle has no quantitative system, causes the difficulty of actual metering operation and is inconvenient to use.

The utility model provides a technical scheme that its technical problem adopted is: the device for preparing and quantitatively subpackaging the anaerobic microorganism culture medium comprises an anaerobic bottle, a stirrer, a quantitative system and a subpackaging bottle, wherein the stirrer is used for stirring in the anaerobic bottle, and the quantitative system is arranged between the subpackaging bottle and the anaerobic bottle and is respectively communicated with the subpackaging bottle and the anaerobic bottle.

Wherein, the ration system includes dosage tube and control valve in the above-mentioned device, the control valve sets up on the pipeline between split charging bottle and the anaerobism bottle, and control valve and dosage tube intercommunication.

Furthermore, the control valve in the device is a three-way valve, two outlets of the control valve are respectively communicated with the quantitative pipe and the anaerobic bottle, and an inlet of the control valve is communicated with the anaerobic bottle.

Wherein, among the above-mentioned device the anaerobism bottle bottleneck is equipped with the stopper, and has worn intake pipe and open closed blast pipe on the stopper, and intake pipe penetrating end level is less than the blast pipe penetrating end.

Wherein, among the above-mentioned device divide the bottling bottleneck to be equipped with the stopper, and worn dress liquid pipe and open closed breather pipe on the stopper, divide the bottling to pass through dress liquid pipe and anaerobism bottle intercommunication, quantitative system sets up on dress liquid pipe.

Furthermore, the device also comprises a split charging cover which is sleeved on the upper part of the split charging bottle, and the liquid charging pipe and the vent pipe penetrate through the split charging cover and are connected with the split charging cover in a sealing way.

Wherein, the side wall of the upper part of the anaerobic bottle in the device is provided with a closable sample adding opening.

Further, in the device, the air inlet pipe is provided with a sterile filter membrane.

The stirrer in the device is a magnetic stirrer, the anaerobic bottle is arranged on the magnetic stirrer, and the metal stirring particles of the magnetic stirrer are arranged at the bottom of the anaerobic bottle.

The utility model has the advantages that: the device has simple structure, low manufacturing cost and convenient operation, and can realize the simultaneous preparation and the subpackage of the culture medium. The device is additionally provided with the stirrer, so that other reaction substrates required by the anaerobic bottle can be added into the reaction system and uniformly mixed, and the aim of preparing the culture medium is fulfilled. Meanwhile, the anaerobic bottle and the split charging bottle are connected through a quantitative system, and the culture amount flowing into the split charging bottle is controlled through the quantitative system, so that the culture medium is quantitatively split charged.

Drawings

Fig. 1 is a schematic view of the connection structure of the present invention.

Labeled as: 1 is an air inlet pipe, 11 is a sterile filter membrane, 2 is an anaerobic bottle, 21 is a sample adding port, 22 is a clamp, 23 is an exhaust pipe, 3 is a stirrer, 31 is metal stirring particles, 4 is a quantitative system, 41 is a control valve, 42 is a quantitative pipe, 5 is a subpackaging bottle, 51 is a subpackaging cover, 52 is a liquid filling pipe, and 53 is an air vent pipe.

Detailed Description

The present invention will be further explained with reference to the accompanying drawings.

As shown in fig. 1, the utility model discloses a preparation and device of ration partial shipment anaerobic microorganism culture medium, including anaerobic bottle 2, agitator 3, ration system 4 and split charging bottle 5, agitator 3 is used for 2 bottles of anaerobic bottle internal stirring, and ration system 4 establishes between split charging bottle 5 and anaerobic bottle 2 to communicate with split charging bottle 5 and anaerobic bottle 2 respectively. As can be understood by those skilled in the art, the anaerobic bottle 2 of the device is mainly used for preparing culture medium, and the structural shape of the anaerobic bottle is consistent with that of the existing anaerobic bottle 2, so long as the bottle mouth is sealed. The stirrer 3 is only required to uniformly mix other reaction substrates required by the reaction system added into the anaerobic bottle 2, and the specific structure of the stirrer can be determined according to the structure of the anaerobic bottle 2. The subpackaging bottle 5 is mainly used for subpackaging culture mediums, the structure of which is not specified, and the bottle mouth sealing can be realized. The quantitative system 4 mainly controls the amount of the culture medium flowing into the split charging bottle 5, so the quantitative system 4 is further arranged between the split charging bottle 5 and the anaerobic bottle 2 and is actually communicated with the split charging bottle 5 and the anaerobic bottle 2 through pipelines respectively, and the control of the amount of the culture medium flowing into the split charging bottle 5 is realized. A micro intake system may be preferably provided in the intake pipe 1 to further control the intake air amount and the gas type. In practice, except the micro air inlet system and the stirrer 3, the materials of other devices are glass and rubber which are resistant to high temperature and high pressure, so that the whole device is placed in a high-pressure steam sterilization pot for sterilization.

Preferably, the quantitative system 4 in the above device comprises a quantitative pipe 42 and a control valve 41, the control valve 41 is arranged on the pipeline between the split charging bottle 5 and the anaerobic bottle 2, and the control valve 41 is communicated with the quantitative pipe 42. Those skilled in the art can understand that the device preferably selects the structure and connection relationship of the dosing system 4, and specifically includes the dosing tube 42 and the control valve 41, and actually sets the control valve 41 on the pipeline between the split charging bottle 5 and the anaerobic bottle 2, and simultaneously ensures that the control valve 41 is communicated with the dosing tube 42, so that the control valve 41 can be switched to realize the communication between the anaerobic bottle 2 and the dosing tube 42 or the communication between the dosing tube 42 and the split charging bottle 5. In order to facilitate the discharge of the anaerobic bottle 2, the preferred access end is located on the side wall of the anaerobic bottle 2 close to the bottom surface, and the anaerobic bottle 2 and the split charging bottle 5 are arranged in a vertical height mode, so that the culture medium can smoothly enter the split charging bottle 5. And the dosing tube 42 of the present device may preferably be a dosing syringe, so that dosing syringes of different volumes may be replaced according to experimental requirements.

Preferably, in the above device, the control valve 41 is a three-way valve, two outlets of the control valve 41 are respectively communicated with the dosing pipe 42 and the anaerobic bottle 2, and an inlet is communicated with the anaerobic bottle 2. As can be understood by those skilled in the art, in order to facilitate installation and use of the device, it is further preferable to define the control valve 41 as a three-way valve, two outlets of the control valve 41 are respectively communicated with the quantitative tube 42 and the anaerobic bottle 2, an inlet is communicated with the anaerobic bottle 2, referring to the attached drawings, that is, the left end and the right end are respectively communicated with the anaerobic bottle 2 and the split charging bottle 5, and the upper end is communicated with the quantitative tube 42, so that the quantitative split charging and the split charging of the culture medium can be realized by actually rotating the three-way valve.

Preferably, in the device, a plug is arranged at the mouth of the anaerobic bottle 2, an air inlet pipe 1 and an exhaust pipe 23 which can be opened and closed are penetrated on the plug, and the horizontal height of the penetrating end of the air inlet pipe 1 is lower than that of the penetrating end of the exhaust pipe 23. As can be understood by the technical personnel in the field, the air inlet pipe 1 can be introduced with various anaerobic gases such as high-purity nitrogen and helium according to the experimental requirements, the gas enters the anaerobic bottle 2 to ensure that the culture medium in the bottle is always in an aseptic and anaerobic state, the bottle mouth of the anaerobic bottle 2 is preferably provided with a plug, the plug is provided with the air inlet pipe 1 and an exhaust pipe 23 which can be opened and closed, and the oxygen can be removed by introducing the oxygen-free nitrogen and helium through the air inlet pipe 1. The exhaust pipe 23 is mainly used for pressure reduction and exhaust, so the exhaust pipe 23 needs to be opened or closed in different states, and the exhaust pipe 23 is preferably a hose or a hose connected with one end, and the exhaust pipe 23 is connected or disconnected by the clamp 22. In order to remove oxygen as much as possible from the culture medium, it is further preferable that the inlet pipe 1 has a lower level than the inlet pipe 23.

Preferably, in the above device, a stopper is provided on the opening of the dispensing bottle 5, a liquid filling tube 52 and an openable and closable vent tube 53 are inserted through the stopper, the dispensing bottle 5 is communicated with the anaerobic bottle 2 through the liquid filling tube 52, and the quantitative system 4 is provided on the liquid filling tube 52. As will be understood by those skilled in the art, in order to facilitate the introduction of the culture medium into the dispensing bottle 5, it is preferable that a stopper is provided on the opening of the dispensing bottle 5, a liquid filling tube 52 and an openable and closable vent tube 53 are inserted through the stopper, the dispensing bottle 5 is connected to the anaerobic bottle 2 through the liquid filling tube 52, and the quantitative system 4 is provided on the liquid filling tube 52. The air inlet pipe 1 and the air vent pipe 53 can be filled with the same or different oxygen-free sterile gas according to experiment requirements, the gas filled in the air inlet pipe 1 is mainly used for discharging oxygen and dissolving gas required by a culture medium, for example, the culture medium prepared with a carbonate buffer solution needs to be dissolved with carbon dioxide, the air vent pipe 53 is mainly used for discharging air in the split charging bottle 5, and oxygen-free gas with lower cost, such as high-purity nitrogen gas and the like, can be selected.

Preferably, the device further comprises a dispensing cover 51, the dispensing cover 51 is sleeved on the upper portion of the dispensing bottle 5, and the liquid filling pipe 52 and the vent pipe 53 penetrate through the dispensing cover 51 and are connected with the dispensing cover in a sealing manner. As can be understood by those skilled in the art, in order to ensure that sterile and anaerobic subpackaging of the culture medium can be realized, the device is further provided with a subpackaging cover 51, the subpackaging cover 51 is sleeved on the upper part of the subpackaging bottle 5, and the liquid filling pipe 52 and the vent pipe 53 penetrate through the subpackaging cover 51 and are connected with the subpackaging cover in a sealing manner. The sterile oxygen-free sub-packaging cover 51 is connected with a vent pipe 53 provided with a sterile film, so that the entry of sterile air can be prevented, and the air in the sub-packaging bottle 5 can be emptied.

Preferably, in the device, a closable sample addition port 21 is arranged on the side wall of the upper part of the anaerobic bottle 2. As will be understood by those skilled in the art, it is preferable to provide the sample addition port 21 on the upper side wall of the anaerobic flask 2 in order to facilitate the timely addition of various sterile medium components.

Preferably, in the above device, the air inlet pipe 1 is provided with a sterile filter membrane 11. As will be understood by those skilled in the art, in order to realize a sterile environment, a sterile filter membrane 11 is further disposed on the gas inlet pipe 1 to ensure that various oxygen-free gases such as high-purity nitrogen gas and helium gas are introduced as sterile gases.

Preferably, in the above device, the stirrer 3 is a magnetic stirrer 3, the anaerobic bottle 2 is placed on the magnetic stirrer 3, and the metal stirring particles 31 of the magnetic stirrer 3 are placed at the bottom of the anaerobic bottle 2. It can be understood by those skilled in the art that the present apparatus is only a specific structure of a preferred stirrer 3, and actually, the stirrer 3 is preferably a magnetic stirrer 3, the magnetic stirrer 3 is an existing apparatus, specifically, the anaerobic bottle 2 is placed on the magnetic stirrer 3, the metallic stirring particles 31 of the magnetic stirrer 3 are placed at the bottom of the anaerobic bottle 2, and the metallic stirring particles 31 are adsorbed by magnetic force to drive the stirring particles to rotate, so as to achieve the purpose of stirring.

The operation method comprises the following steps: after the device is assembled, the control valve 41 is closed to add the anaerobic microorganism culture medium into the anaerobic bottle 2, and each interface and the gas-liquid inlet and outlet are wrapped by the tin foil paper, the tin foil paper is wrapped after the sample adding port 21 and the cover of the anaerobic bottle 2 are slightly unscrewed, except the micro gas inlet system and the stirrer 3 on the gas inlet pipe 1, the rest parts are placed in the high-pressure steam sterilization pot for sterilization. After the completion, the taking-out device is immediately communicated with the micro air intake system, the sample port 21 and the cover of the anaerobic bottle 2 are screwed down, the clamp 22 on the rubber tube of the exhaust tube 23 is opened, and the vent tube 53 can be communicated with anaerobic gas before the anaerobic culture medium is subpackaged. If other nutrient components are required to be added into the anaerobic culture medium before subpackaging, such as glucose and the like which can not be sterilized at high temperature and high pressure, the exhaust pipe 23 can be closed by the clamp 22, and the sample adding opening 21 is opened, so that the sterile oxygen-free gas is discharged from the sample adding opening 21, and air is prevented from entering the anaerobic bottle 2. After the sample is added, the sample adding port 21 is closed and the clamp 22 is opened, then the stirrer 3 is opened, and the solution is prepared for subpackaging after being uniformly mixed. Opening the vent pipe 53, placing the sterile subpackaging bottle 5 in the sterile anaerobic subpackaging cover 51, removing air in the sterile subpackaging bottle, adjusting the control valve 41 to connect the liquid outlet at the bottom of the anaerobic bottle 2 with the quantitative system 4, taking a quantitative anaerobic culture medium, and closing the control valve 41. Then, the control valve 41 is adjusted again to enable the quantitative system 4 and the liquid filling pipe 52 to be combined, the anaerobic culture medium is subpackaged into the subpackaging bottle 5, then the sterile subpackaging bottle 5 is covered with a rubber cover, the subpackaging is completed for one time, and the operation is repeated until the subpackaging is completed.

Claims

1. Prepare and quantitative partial shipment anaerobic microorganisms culture medium's device, characterized by: the anaerobic bottle quantitative device comprises an anaerobic bottle (2), a stirrer (3), a quantitative system (4) and a split charging bottle (5), wherein the stirrer (3) is used for stirring in the anaerobic bottle (2), and the quantitative system (4) is arranged between the split charging bottle (5) and the anaerobic bottle (2) and is respectively communicated with the split charging bottle (5) and the anaerobic bottle (2).

2. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 1, wherein: the quantitative system (4) comprises a quantitative pipe (42) and a control valve (41), the control valve (41) is arranged on a pipeline between the split charging bottle (5) and the anaerobic bottle (2), and the control valve (41) is communicated with the quantitative pipe (42).

3. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 2, wherein: the control valve (41) is a three-way valve, two outlets of the control valve (41) are respectively communicated with the quantitative pipe (42) and the anaerobic bottle (2), and an inlet of the control valve is communicated with the anaerobic bottle (2).

4. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 1, wherein: the mouth of the anaerobic bottle (2) is provided with a plug, an air inlet pipe (1) and an openable exhaust pipe (23) are arranged on the plug in a penetrating mode, and the air inlet pipe (1) penetrates through the end level height to be lower than the penetrating end of the exhaust pipe (23).

5. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium as set forth in any one of claims 1 to 4, wherein: the bottle mouth of the split charging bottle (5) is provided with a plug, a liquid charging pipe (52) and an openable and closable vent pipe (53) are arranged on the plug in a penetrating mode, the split charging bottle (5) is communicated with the anaerobic bottle (2) through the liquid charging pipe (52), and the quantifying system (4) is arranged on the liquid charging pipe (52).

6. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 5, wherein: the packaging bottle is characterized by further comprising a sub-packaging cover (51), the sub-packaging cover (51) is sleeved on the upper portion of the sub-packaging bottle (5), and the liquid filling pipe (52) and the vent pipe (53) penetrate through the sub-packaging cover (51) and are in sealing connection with the sub-packaging cover.

7. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 1, wherein: the side wall of the upper part of the anaerobic bottle (2) is provided with a closable sample adding opening (21).

8. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 4, wherein: the air inlet pipe (1) is provided with a sterile filter membrane (11).

9. The apparatus for preparing and quantitatively dispensing an anaerobic microorganism culture medium according to claim 1, wherein: the stirrer (3) is a magnetic stirrer (3), the anaerobic bottle (2) is arranged on the magnetic stirrer (3), and the metal stirring particles (31) of the magnetic stirrer (3) are arranged at the bottom of the anaerobic bottle (2).