CN217535994U - Liquid device is got in wet fermentation of anaerobism - Google Patents
Liquid device is got in wet fermentation of anaerobism Download PDFInfo
- Publication number
- CN217535994U CN217535994U CN202221228026.8U CN202221228026U CN217535994U CN 217535994 U CN217535994 U CN 217535994U CN 202221228026 U CN202221228026 U CN 202221228026U CN 217535994 U CN217535994 U CN 217535994U
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- fermentation
- liquid
- bottle
- holes
- stainless steel
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- 238000000855 fermentation Methods 0.000 title claims abstract description 61
- 239000007788 liquid Substances 0.000 title claims abstract description 58
- 230000004151 fermentation Effects 0.000 title claims abstract description 54
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 21
- 239000010935 stainless steel Substances 0.000 claims abstract description 21
- 238000007789 sealing Methods 0.000 claims abstract description 11
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims abstract description 5
- 239000000741 silica gel Substances 0.000 claims abstract description 5
- 229910002027 silica gel Inorganic materials 0.000 claims abstract description 5
- 239000011148 porous material Substances 0.000 claims 1
- 238000005070 sampling Methods 0.000 abstract description 17
- 230000000813 microbial effect Effects 0.000 abstract description 3
- 244000005700 microbiome Species 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 239000012530 fluid Substances 0.000 abstract 1
- 239000002699 waste material Substances 0.000 abstract 1
- 239000007787 solid Substances 0.000 description 6
- 238000000034 method Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 206010039509 Scab Diseases 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000003851 biochemical process Effects 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000010806 kitchen waste Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000000717 retained effect Effects 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an anaerobic fermentation field specifically indicates a liquid device is got in wet fermentation of anaerobism includes upper shed fermentation bottle, sealing member and liquid sampling component. The sealing component comprises a silica gel gasket, two stainless steel through holes and a fixing cover, and the two through holes and the fixing cover are sequentially arranged at the opening of the fermentation bottle to realize the sealing of the interior of the fermentation bottle. The two through holes of the stainless steel are respectively arranged as a liquid taking hole and an exhaust hole, the liquid taking hole in the bottle body is connected to the small filter through a hose, and the distance between the liquid taking hole and the small filter is two fifths of the height of the small filter. The upper ends of the two stainless steel through holes are connected with two hoses, one hose is an exhaust pipe, and the other hose is a liquid taking pipe. The air outlet of the bottle body is connected with an air bag, a manual valve is arranged at the liquid outlet of the bottle body, and the air bag, a sealing part and the fermentation bottle are arranged together to form a closed environment. The utility model provides a liquid device is got in anaerobism wet fermentation can solve general liquid operation difficulty, waste zymotic fluid scheduling problem of getting, has guaranteed the required anaerobic environment of microorganism in the bottle, has reduced the environmental impact of extracting liquid sample to microbial fermentation; the operation is simple, the sampling time is saved, and the sampling efficiency is improved.
Description
Technical Field
The utility model relates to an anaerobic fermentation field specifically is a liquid device is got in wet fermentation of anaerobism for quick sample of wet-type anaerobic fermentation liquid sample.
Background
Anaerobic fermentation is a complex biochemical process, in which organic substances (livestock and poultry manure, straws, kitchen waste and the like) are decomposed and metabolized by various microorganisms in a proper anaerobic environment to finally form methane and carbon dioxide. Depending on the solid content of the fermentation feedstock, the fermentation can be divided into soluble fermentation, low solids concentration fermentation and high solids concentration fermentation. The solids content in the fermentation flask and the particle size of the feedstock are the main factors affecting the sampling during the fermentation process.
Regular sampling is an important means for determining the operating parameter index in the anaerobic fermentation process. In the fermentation process, the fermented product floats upwards seriously and crusts, and the solid concentration of the mixed material is high, so that the sampling operation is difficult. When the anaerobic fermentation characteristic is researched in a laboratory, a fermentation liquid sample in a fermentation bottle needs to be taken out regularly for monitoring, however, the sampling pipe is easy to be blocked due to the problems of high solid concentration of raw materials and the like during sampling, or the sampling is carried out by opening the bottle, but the anaerobic environment in the fermentation bottle can be damaged. To above-mentioned sample actual problem, this patent provides a get liquid device that is used for low concentration wet-type fermentation in laboratory to simple and convenient sample process, save the sample time.
Disclosure of Invention
The utility model aims to overcome the defects of difficult sampling of laboratory fermentation bottles and the like and provides a liquid taking device for anaerobic wet fermentation.
The utility model discloses a realize through following technical principle and scheme:
the anaerobic wet fermentation liquid taking device is characterized by comprising a fermentation bottle with an upper opening, a sealing part and a liquid sampling part. The sealing component comprises a silica gel gasket, two stainless steel through holes and a fixing cover, and the two through holes are sequentially arranged at the opening of the fermentation bottle to realize the sealing of the interior of the fermentation bottle. The two through holes of the stainless steel are respectively arranged as a liquid taking hole and an exhaust hole, the liquid taking hole in the bottle body is connected to the small filter through a hose, and the distance between the liquid taking hole and the small filter is two fifths of the height of the small filter. Two hoses are connected to the upper ends of the two through holes of the stainless steel, one hose is an exhaust pipe, the other hose is a liquid taking pipe, and an injector is connected to the tail end of the hose during sampling.
The aperture of the small filter is 40-60 meshes and is made of plastic.
The hole diameters of the two stainless steel through holes can be set in proportion according to the volume of the fermentation bottle, but the diameters of the two stainless steel through holes are not less than 5 mm.
The upper ends of the two stainless steel through holes of the sealing part are connected with the air bag through a hose, the hose is communicated with the top space of the fermentation bottle through the two stainless steel through holes, and gas generated in the bottle body enters the air bag through the hose.
The other end of the upper end of the two through holes of the stainless steel is a liquid taking pipe, a manual valve is arranged at a liquid taking hose, and the hose extends into the bottom in the small filter.
The utility model provides a pair of liquid device is got in wet fermentation of anaerobism has following advantage:
(1) The anaerobic environment required by the microorganisms in the fermentation bottle is ensured in the sampling process, and the environmental influence of the extracted liquid sample on the microbial fermentation is reduced;
(2) The operation is simple, the sampling time is saved, and the sampling efficiency is improved.
Drawings
FIG. 1 is a front view of the device of the present invention before sampling;
FIG. 2 is a front view of the present invention when a liquid sample is drawn;
wherein the reference numbers are: 1 is liquid taking pipe upper portion, 2 is the air outlet valve, 3 is fixed lid, 4 is two through-holes of stainless steel, 5 is the silica gel gasket, 6 is the exhaust hole, 7 is the fermentation bottle, 8 is the anaerobic fermentation mixed liquid, 9 is liquid taking pipe lower part, 10 is small-size filter, 11 is the syringe, 12 is the liquid sample that has taken out, 13 is liquid taking pipe valve.
Detailed Description
An anaerobic wet fermentation liquid taking device comprises a fermentation bottle 7, and anaerobic fermentation mixed liquid 8 is filled into the fermentation bottle 7. The upper part 1 of the liquid taking pipe, the lower part 9 of the liquid taking pipe and the exhaust pipe are connected with the two stainless steel through holes 4. The lower part 9 of the liquid taking pipe is connected with a small filter 10, and the end of the liquid taking pipe 9 needs to be arranged inside the filter. And then the fixed cover 3, the two stainless steel through holes 4 and the silica gel gasket 5 are sequentially arranged at the opening of the fermentation bottle, so that the inner part of the fermentation bottle is sealed. After the installation is finished, a proper amount of nitrogen is introduced into the device, oxygen in the bottle is discharged, and the anaerobic environment of microbial fermentation is ensured. And finally, closing the valve 13 of the liquid taking pipe, connecting the air bag, opening the air outlet valve 2, and putting the fermentation bottle into a constant-temperature incubator for fermentation culture.
In the shaking process of the fermentation bottle, the mixture in the bottle can pass through the small filter 10, and liquid substances at the back of shaking can be retained in the filter. When the liquid is taken, the gas outlet valve 2 is kept opened, the injector 11 is connected with the upper part 1 of the liquid taking tube, the liquid taking tube valve 13 is opened, the injector 11 is used for pumping the liquid in the small filter 10 to the required volume, the liquid taking tube valve 13 is closed, the injector 11 is taken down, and the liquid sample in the injector 11 is injected into the sample tube to finish sampling.
The above contents are only for explaining the technical idea of the present invention, and the protection scope of the present invention cannot be limited thereby, and any modification made on the basis of the technical solution according to the technical idea of the present invention all fall within the protection scope of the claims of the present invention.
Claims (5)
1. The utility model provides a wet fermentation of anaerobism gets liquid device, a serial communication port, including the upper shed fermentation bottle, sealing element and liquid sample unit, sealing element includes the silica gel gasket, two through-holes of stainless steel and fixed lid, install in proper order at the fermentation bottleneck, realize the inside sealed of fermentation bottle, two through-holes of stainless steel are established respectively and are got liquid hole and exhaust hole, get liquid hole through hose connection to small-size filter in the bottle, it is apart from the high two fifths department at the bottom of the bottle, two hoses of two through-hole upper end connections of stainless steel, one is the blast pipe, another is for getting the liquid pipe, the syringe inserts the hose end during the sample.
2. The anaerobic wet fermentation liquid taking device as claimed in claim 1, wherein the pore size of the small filter is 40-60 meshes and is made of plastic.
3. The anaerobic wet fermentation liquid taking device as claimed in claim 1, wherein the hole diameter of the stainless steel two through holes can be set proportionally according to the volume of the fermentation bottle, but the diameter is not less than 5 mm.
4. The liquid taking device for anaerobic wet fermentation according to claim 1, wherein a hose is connected to the upper end of the two stainless steel through holes of the sealing member, the hose is communicated with the top space of the fermentation bottle through the two stainless steel through holes, and the gas generated in the bottle body enters the air bag through the hose.
5. The anaerobic wet fermentation liquid taking device as claimed in claim 1, wherein the other of the upper ends of the two stainless steel through holes is a liquid taking pipe, a manual valve is arranged at a liquid taking hose, and the hose extends into the bottom in the small filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221228026.8U CN217535994U (en) | 2022-05-22 | 2022-05-22 | Liquid device is got in wet fermentation of anaerobism |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202221228026.8U CN217535994U (en) | 2022-05-22 | 2022-05-22 | Liquid device is got in wet fermentation of anaerobism |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN217535994U true CN217535994U (en) | 2022-10-04 |
Family
ID=83442103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202221228026.8U Expired - Fee Related CN217535994U (en) | 2022-05-22 | 2022-05-22 | Liquid device is got in wet fermentation of anaerobism |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN217535994U (en) |
-
2022
- 2022-05-22 CN CN202221228026.8U patent/CN217535994U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20221004 |
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| CF01 | Termination of patent right due to non-payment of annual fee |