CN209652297U - A kind of multidirectional air pressure drainage set - Google Patents
A kind of multidirectional air pressure drainage set Download PDFInfo
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- CN209652297U CN209652297U CN201920021712.XU CN201920021712U CN209652297U CN 209652297 U CN209652297 U CN 209652297U CN 201920021712 U CN201920021712 U CN 201920021712U CN 209652297 U CN209652297 U CN 209652297U
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- outlet tube
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- tube
- air inlet
- air pressure
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- 239000007788 liquid Substances 0.000 claims abstract description 54
- 229910001220 stainless steel Inorganic materials 0.000 claims abstract description 33
- 239000010935 stainless steel Substances 0.000 claims abstract description 33
- 239000012530 fluid Substances 0.000 claims abstract description 16
- 229920001296 polysiloxane Polymers 0.000 claims description 9
- 229910000831 Steel Inorganic materials 0.000 claims description 4
- 239000010959 steel Substances 0.000 claims description 4
- 239000012930 cell culture fluid Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 8
- 239000000725 suspension Substances 0.000 description 7
- 239000000243 solution Substances 0.000 description 6
- 230000001376 precipitating effect Effects 0.000 description 4
- 238000001556 precipitation Methods 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- 238000004659 sterilization and disinfection Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000009630 liquid culture Methods 0.000 description 3
- 241000700605 Viruses Species 0.000 description 2
- 238000002347 injection Methods 0.000 description 2
- 239000007924 injection Substances 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 239000002574 poison Substances 0.000 description 2
- 231100000614 poison Toxicity 0.000 description 2
- 238000003825 pressing Methods 0.000 description 2
- 230000003068 static effect Effects 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000003636 conditioned culture medium Substances 0.000 description 1
- 230000008878 coupling Effects 0.000 description 1
- 238000010168 coupling process Methods 0.000 description 1
- 238000005859 coupling reaction Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 238000005096 rolling process Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 238000012549 training Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A kind of multidirectional air pressure drainage set, is related to field of biotechnology.The utility model includes drain bottle；Mobile phase Three hole, mobile phase Three hole are equipped with air inlet, fluid hole, inlet opening；The air inlet pipe in drain bottle is inserted by air inlet；The filter disc being located in air inlet pipe；It is inserted into drain bottle by fluid hole and goes out liquid share pipe；The stainless steel inverted triangle device for being located at out liquid share pipe lower end and being located inside drain bottle；The first outlet tube and the second outlet tube being connected with liquid share pipe upper end out；It is located at the stainless steel funnel of the first outlet tube outside port；It is located at the first stainless steel fast joint of the second outlet tube outside port；The inlet tube in drain bottle is inserted by inlet opening；It is located at the second stainless steel fast joint of inlet tube outside port；Valve is equipped on air inlet pipe, the first outlet tube, the second outlet tube and inlet tube.The utility model can multi-purpose content, simultaneously have many advantages, such as save manpower, work efficiency is high, pollution risk is small.
Description
Technical field
The utility model relates to field of biotechnology, and in particular to a kind of multidirectional air pressure drainage set.
Background technique
Currently, especially cell suspension cultures, which are changed, amplifies training in terms of biotechnology test between liquid and bioreactor
During supporting, drainage set is often used.In existing drainage set, conduit coupling belongs to fixed pipeline connection
Mode.When drain mode, drain amount etc. need to change, an independent drainage set cannot meet different tests demand simultaneously.
Therefore, a test need to prepare multiple drainage sets, not only cumbersome, but also increase the risk of bacterium infection.
In view of the above-mentioned problems, needing to be transformed existing drainage set, various test demands are not only able to satisfy, but also can be reduced
Operation reduces pollution.
Utility model content
In view of the above-mentioned problems, the utility model provides, a kind of structure is simple, can be suitable for the multidirectional of different tests demand
Air pressure drainage set.
Used technical solution is as follows in order to solve the technical problem for the utility model:
A kind of multidirectional air pressure drainage set of the utility model, comprising:
Drain bottle；
The mobile phase Three hole being screwed on drain bottle, be provided on the mobile phase Three hole air inlet, fluid hole, into
Fluid apertures；
The air inlet pipe in drain bottle is inserted by the air inlet of mobile phase Three hole；
Filter disc in air inlet pipe is set；
It is inserted into drain bottle by the fluid hole of mobile phase Three hole and goes out liquid share pipe；
The stainless steel inverted triangle device that liquid share pipe lower end out is set and is located inside drain bottle；
The first outlet tube and the second outlet tube being connected with liquid share pipe upper end out；
The stainless steel funnel of first outlet tube outside port is set；
First stainless steel fast joint of the second outlet tube outside port is set；
The inlet tube in drain bottle is inserted by the inlet opening of mobile phase Three hole；
Second stainless steel fast joint of inlet tube outside port is set；
Valve is provided on air inlet pipe, the first outlet tube, the second outlet tube and inlet tube.
Further, the bottleneck diameter of the drain bottle is 45mm, volume 2000ml, 5000ml or 10000ml.
Further, the air inlet pipe is silicone tube, and 5-8cm in drain bottle is protruded into lower end.
Further, the air inlet pipe air inlet it is external, described go out liquid share pipe fluid hole it is external, described into
Liquid pipe is set with buckle in the outside of inlet opening.
Further, the liquid share pipe out is steel pipe；The length of the liquid share pipe out is greater than bottle body height 4-6cm.
Further, the length of the liquid share pipe in drain bottle out can be adjusted by the buckle outside liquid outlet.
Further, the inlet tube is silicone tube, and 8-10cm in drain bottle 1 is protruded into lower end.
Further, first outlet tube and the equal silicone tube of the second outlet tube.
Further, the filter disc uses 0.22um filter.
Further, the air inlet pipe outside port connects air pump.
The beneficial effects of the utility model are:
1, a kind of multidirectional air pressure drainage set of the utility model is mainly used in field of biotechnology, such as sterile drain,
Connection between multiple bioreactors and uniformly dispense liquid more particularly to a kind of Telescopic-type pipeline connection device.
2, a kind of multidirectional air pressure drainage set of the utility model can be according to different drain needs, telescopic regulation pipe
Road length does not need to replace other drainage sets, saves manpower, improve working efficiency, reduces pollution risk.
3, a kind of multidirectional air pressure drainage set of the utility model can go out liquid share pipe by adjusting in bottle in fluid hole
Length be discharged the liquid of different volumes, adapt to different test needs.
4, in a kind of multidirectional air pressure drainage set of the utility model, at the upward 1cm of steel pipe bottom in fluid hole, there is one
Stainless steel inverted triangle device, it is possible to reduce suction causes damages to avoid to the substance (such as cell) for needing reservation precipitating.
5, a kind of multidirectional air pressure drainage set of the utility model can be with multi-purpose content, while can save manpower, improves
Working efficiency reduces pollution risk.
Detailed description of the invention
Fig. 1 is a kind of structural schematic diagram of multidirectional air pressure drainage set of the utility model.
In figure: 1, drain bottle, 2, mobile phase Three hole, 3, air inlet pipe, the 4, first outlet tube, the 5, second outlet tube, 6, into
Liquid pipe, 7, stainless steel funnel, the 8, first stainless steel fast joint, the 9, second stainless steel fast joint, 10, stainless steel inverted triangle device,
11, valve, 12, filter disc, 13, go out liquid share pipe, 14, buckle.
Specific embodiment
The utility model is described in further detail below in conjunction with drawings and examples.
As shown in Figure 1, a kind of multidirectional air pressure drainage set of the utility model specifically includes that drain bottle 1, three hole of mobile phase
Lid 2 and three-way pipeline device.Mobile phase Three hole 2 is screwed in 1 upper end of drain bottle.Three-way pipeline device is fixed on three hole of mobile phase
On lid 2.
In the utility model, drain bottle 1 is the standard blue lid reagent bottle of bottleneck diameter 45mm.Specially there is opening in upper end
Single port bottle has other bottle bodies of opening in the two sidewalls of upper end opening.The volume of drain bottle 1 be 2000ml, 5000ml or
10000ml etc..
Air inlet, fluid hole, inlet opening are provided on mobile phase Three hole 2.
Three-way pipeline device include: air inlet pipe 3, the first outlet tube 4, the second outlet tube 5, inlet tube 6, stainless steel funnel 7,
First stainless steel fast joint 8,10, four valves 11 of stainless steel inverted triangle device, filter disc 12, goes out liquid at second stainless steel fast joint 9
Share pipe 13 and three buckles 14.
Air inlet pipe 3 is inserted into drain bottle 1 by the air inlet of mobile phase Three hole 2.Air inlet pipe 3 is in the outside of air inlet
It is set with buckle 14, prevents air inlet pipe 3 arbitrarily mobile.A filter disc 12 and a valve 11 are provided in air inlet pipe 3, filter disc 12 is adopted
With 0.22um filter, for removing bacterium, valve 11 is for controlling gas disengaging.3 outside port of air inlet pipe connects air pump, beats
Open air pump and valve 11, by filter disc 12 into drain bottle 1 sterile gas injection, make the liquid in drain bottle 1 in the work of air pressure
With outside lower discharge bottle.
In the utility model, air inlet pipe 3 is a silicone tube, and 5-8cm in drain bottle 1 is protruded into lower end.Air inlet pipe 3 passes through filter
Disk 12 is connected with air pump, to guarantee that the gas poured by filter disc 12 into drain bottle 1 is sterile.
Liquid share pipe 13 is inserted into drain bottle 1 by the fluid hole of mobile phase Three hole 2 out.Liquid share pipe 13 is going out out
The outer cover of fluid apertures can adjust out length of the liquid share pipe 13 in drain bottle 1 by buckle equipped with buckle 14.Liquid shares out
13 lower end of pipe is provided with a stainless steel inverted triangle device 10 (being located inside drain bottle 1).13 upper end of liquid share pipe is provided with two out
A branch, respectively the first outlet tube 4 and the second outlet tube 5.Valve 11 is provided on first outlet tube 4, the first outlet tube 4
Outside port is provided with a stainless steel funnel 7.Valve 11 is provided on second outlet tube 5, the outside port of the second outlet tube 5 is set
It is equipped with one first stainless steel fast joint 8.Under the action of gas pressure, the liquid in drain bottle 1 is by going out liquid share pipe 13,
Outside one outlet tube 4 and the second outlet tube 5 discharge bottle.
In the utility model, liquid share pipe 13 is a steel pipe out.Liquid share pipe 13 is exposed outside mobile phase Three hole 2 out
Length be 4-6cm.
In the utility model, the first outlet tube 4 and the equal silicone tube of the second outlet tube 5.It can be passed through according to different tests demand
Buckle adjusts out the outlet tube of length or selection different branches of the liquid share pipe 13 in drain bottle 1 upward or downward, to meet
Different tests needs.Meanwhile first outlet tube 4 and the second outlet tube 5 also to can be used as inlet tube spare.
Inlet tube 6 is inserted into drain bottle 1 by the inlet opening of mobile phase Three hole 2.Inlet tube 6 is in the outside of inlet opening
It is set with a buckle 14, prevents inlet tube 6 arbitrarily mobile.Valve 11 is provided on inlet tube 6.The outside port of inlet tube 6 is set
It is equipped with one second stainless steel fast joint 9.Liquid is injected into drain bottle 1 by inlet tube 6.
In the utility model, inlet tube 6 is a silicone tube, and 8-10cm in drain bottle 1 is protruded into lower end.According to drain bottle 1
Volume adjusts the medium position of inlet tube 6 to drain bottle 1.
A kind of multidirectional air pressure drainage set of the utility model can carry out 121 DEG C of high pressure sterilization processing before use.
Below in conjunction with the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out clear, complete
Site preparation description, it is clear that the described embodiments are only a part of the embodiments of the utility model, instead of all the embodiments.
Based on the embodiments of the present invention, obtained by those of ordinary skill in the art without making creative efforts
Every other embodiment, fall within the protection scope of the utility model.
Embodiment 1
The utility model is used to utilize bioreactor culture suspension cell, and the connection and cell between two reactors are put
When big culture, concrete operations are as follows: inlet tube 6 being connected to multidirectional air pressure drainage set after 121 DEG C of high pressure sterilizations first
The the second stainless steel fast joint 9 connect is connected with small volume reactor, the valve of air inlet pipe 3 and the first outlet tube 4, the second outlet tube 5
Door 11 is turned off, and the cell culture fluid in small volume reactor is all discharged into the drain bottle 1 of multidirectional air pressure drainage set；
Then the first stainless steel fast joint 8 of the second outlet tube 5 is connected with large volume reactor, the valve 11 of inlet tube 6 is closed,
The stainless steel inverted triangle device 10 in bottle is stretched by pressing down on out liquid share pipe 13 outside the multidirectional air pressure drainage set
Enter to the cell culture fluid bottom, filtrated air is passed through into drain bottle 1 by the filter disc 12 in air inlet pipe 3, to exclude whole
Cell culture fluid is into large volume reactor.The transfer of cell culture fluid between two reactors is completed with this, is realized and is suspended carefully
Born of the same parents amplify culture.
Embodiment 2
The utility model changes liquid culture for suspension cell in shaking flask, and concrete operations are as follows: first leading multidirectional air pressure
Liquid device is spare after 121 DEG C of high pressure sterilizations.The suspension cell of shaking flask culture is placed in static complete to cell in 4 DEG C of environment
Precipitating, shaking flask lid is replaced, nothing is poured into bottle by the filter disc 12 in air inlet pipe 3 with the three-way pipeline device of the utility model
Bacterium air, 4 valve of the first outlet tube is opened, the valve 11 of the second outlet tube 5 is closed, and adjusts out liquid share pipe 13 upward or downward
It is placed exactly in the stainless steel inverted triangle device 10 in bottle among cell precipitation and culture solution, being allowed to can be from fluid hole maximum limit
The discharge cell culture fluid of amount and not drain cell precipitate；Appropriate new culture solution is added from inlet tube 6 again later, cell is resuspended
Precipitating, changes liquid purpose to reach.
Embodiment 3
The utility model changes liquid culture for suspension cell in bioreactor, and concrete operations are as follows: first will be multidirectional
Air pressure drainage set is spare after 121 DEG C of high pressure sterilizations.Second stainless steel fast joint 9 of inlet tube 6 is connected with reactor, into
Tracheae 3, the first outlet tube 4, the second outlet tube 5 valve 11 be turned off, by the cell culture fluid in reactor be all discharged into
In drain bottle 1；By the multidirectional air pressure drainage set be placed in it is static in 4 DEG C of environment precipitated completely to cell, adjust upward or downward
Liquid share pipe 13 is placed exactly in the stainless steel inverted triangle device 10 in bottle among cell precipitation and culture solution out, and being allowed to can be from
The discharge cell culture fluid of fluid hole maximum limitation and not drain cell precipitating；Close the valve of the second outlet tube 5, inlet tube 6
Door 11, by 3 gas injection of air inlet pipe, cell conditioned medium is discharged from the first outlet tube 4；It is added in right amount from inlet tube 6 again later
Cell precipitation is resuspended in new culture solution；Finally the first stainless steel fast joint 8 of the second outlet tube 5 is connected with reactor, first goes out
Liquid pipe 4, inlet tube 6 valve 11 be turned off, by pressing down on out liquid share pipe 13 outside the multidirectional air pressure drainage set
So that the stainless steel inverted triangle device 10 in bottle is extend into the cell culture fluid bottom, all cell re-suspension liquids are discharged to reactor
In, to realize that reactor inner cell changes the purpose of liquid culture.
Embodiment 4
The utility model changes liquid for suspension cell in shaking flask or in reactor and connects poison, and concrete operations are as follows: according to reality
It applies example 2 and embodiment 3 carries out the suspension cell in shaking flask or in reactor to change liquid, when new culture solution resuspension cell precipitation is added
Virus is added, connects poison to achieve the purpose that change liquid.
Embodiment 5
When the utility model is for averagely packing liquid, under concrete operations enter: liquid to be packed is injected in the device,
The valve 11 for closing the second outlet tube 5, inlet tube 6 makes liquid go out liquid by first by injecting filtrated air to air inlet pipe 3
Pipe 4 is evenly distributed in each required device, be ensure that liquid consistency, is reduced differences between batches.Such as cell (reactor and rolling bottle)
By different proportion amplification culture, seed seed culture of viruses be averagely dispensed into each bottle freeze, after several liquid blendings again packing etc..
The above is only the preferred embodiment of the utility model, it is noted that for the common skill of the art
For art personnel, without departing from the principle of this utility model, several improvements and modifications can also be made, these improve and
Retouching also should be regarded as the protection scope of the utility model.
Claims (9)
1. a kind of multidirectional air pressure drainage set characterized by comprising
Drain bottle；
The mobile phase Three hole being screwed on drain bottle is provided with air inlet, fluid hole, feed liquor on the mobile phase Three hole
Hole；
The air inlet pipe in drain bottle is inserted by the air inlet of mobile phase Three hole；
Filter disc in air inlet pipe is set；
It is inserted into drain bottle by the fluid hole of mobile phase Three hole and goes out liquid share pipe；
The stainless steel inverted triangle device that liquid share pipe lower end out is set and is located inside drain bottle；
The first outlet tube and the second outlet tube being connected with liquid share pipe upper end out；
The stainless steel funnel of first outlet tube outside port is set；
First stainless steel fast joint of the second outlet tube outside port is set；
The inlet tube in drain bottle is inserted by the inlet opening of mobile phase Three hole；
Second stainless steel fast joint of inlet tube outside port is set；
Valve is provided on air inlet pipe, the first outlet tube, the second outlet tube and inlet tube.
2. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the bottleneck diameter of the drain bottle
For 45mm, volume 2000ml, 5000ml or 10000ml.
3. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the air inlet pipe is silicone tube,
5-8cm in drain bottle is protruded into lower end.
4. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the air inlet pipe is in air inlet
External, the described liquid share pipe out is set with buckle in the outside of inlet opening in external, the described inlet tube of fluid hole.
5. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the liquid share pipe out is steel
Pipe；The length of the liquid share pipe out is greater than bottle body height 4-6cm.
6. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the inlet tube is silicone tube,
8-10cm in drain bottle is protruded into lower end.
7. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that first outlet tube and second
The equal silicone tube of outlet tube.
8. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the filter disc uses 0.22um mistake
Filter.
9. a kind of multidirectional air pressure drainage set according to claim 1, which is characterized in that the air inlet pipe outside port connects
Connect air pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920021712.XU CN209652297U (en) | 2019-01-07 | 2019-01-07 | A kind of multidirectional air pressure drainage set |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920021712.XU CN209652297U (en) | 2019-01-07 | 2019-01-07 | A kind of multidirectional air pressure drainage set |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209652297U true CN209652297U (en) | 2019-11-19 |
Family
ID=68520348
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920021712.XU Active CN209652297U (en) | 2019-01-07 | 2019-01-07 | A kind of multidirectional air pressure drainage set |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209652297U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
| US11851236B2 (en) | 2021-03-31 | 2023-12-26 | Echem Solutions Corp. | Pipe connectable plastic bucket and method of using the same |
-
2019
- 2019-01-07 CN CN201920021712.XU patent/CN209652297U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US11851236B2 (en) | 2021-03-31 | 2023-12-26 | Echem Solutions Corp. | Pipe connectable plastic bucket and method of using the same |
| CN115232713A (en) * | 2022-09-02 | 2022-10-25 | 安徽大学 | Portable laboratory simple and easy device of oxygen in getting rid of culture medium |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A multi direction pneumatic liquid guiding device Effective date of registration: 20210317 Granted publication date: 20191119 Pledgee: China Everbright Bank Limited by Share Ltd. Changchun branch Pledgor: JL TEYAN BIOLOGICAL TECHNOLOGY LLC Registration number: Y2021220000023 |
|
| PC01 | Cancellation of the registration of the contract for pledge of patent right | ||
| PC01 | Cancellation of the registration of the contract for pledge of patent right |
Granted publication date: 20191119 Pledgee: China Everbright Bank Limited by Share Ltd. Changchun branch Pledgor: JL TEYAN BIOLOGICAL TECHNOLOGY LLC Registration number: Y2021220000023 |
|
| PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
| PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of utility model: A multi-directional pneumatic liquid delivery device Granted publication date: 20191119 Pledgee: Bank of Jilin Co.,Ltd. Changchun New District Sub branch Pledgor: JL TEYAN BIOLOGICAL TECHNOLOGY LLC Registration number: Y2024220000111 |