CN113462570A

CN113462570A - High-capacity suspension cell culture bottle

Info

Publication number: CN113462570A
Application number: CN202110782370.5A
Authority: CN
Inventors: 陈皓; 秦旭
Original assignee: Innovel Intelligent Technology Suzhou Co Ltd
Current assignee: Innovel Intelligent Technology Suzhou Co Ltd
Priority date: 2021-07-12
Filing date: 2021-07-12
Publication date: 2021-10-01
Anticipated expiration: 2041-07-12
Also published as: CN113462570B

Abstract

The invention relates to the technical field of biological pharmacy devices, in particular to a high-capacity suspension cell culture bottle, which comprises a culture bottle body, wherein an upper breathable membrane body is arranged inside the upper side of the culture bottle body, breathable membrane holes distributed in the circumferential direction are formed in the base surface of the culture bottle body, a fully-closed bottle cap assembly is arranged in the middle of the upper side of the culture bottle body, a lower breathable membrane body is arranged on the inner wall of the lower side of the culture bottle body, a culture bottle bottom cover is arranged at the bottom of the culture bottle body, the fully-closed bottle cap assembly comprises a culture bottle cap, a sealing gasket, an upper connecting pipe, a lower connecting pipe and a joint, the sealing gasket is arranged inside the culture bottle cap, the upper connecting pipe is arranged on the fully-closed bottle cap assembly, high cell culture survival rate and effective amplification speed are realized, and cell standing culture and vibration culture are simultaneously satisfied, the process flexibility of different cell types is satisfied.

Description

High-capacity suspension cell culture bottle

Technical Field

The invention relates to the technical field of biological pharmacy devices, in particular to a high-capacity suspension cell culture bottle.

Background

Biological medicine preparation is in the fast development phase at present, in the cultured cell, can use various blake bottles, large capacity blake bottle is in the cell culture in-process on the market at present, the cell carries out gas exchange efficiency poor, can bear the weight of cell density low, it is lower to grow and the transfection equivalent energy, need a large amount of blake bottles or blake bags in order to reach production efficiency target, influence clinical cell production efficiency, traditional blake bottle generally needs the operation of uncapping in operation, be difficult to satisfy new aseptic production's higher standard, need the blake bottle can realize the ability of airtight production, traditional blake bottle blake bag generally is fit for static culture, because the structure restriction can form very big shearing force when shake culture, the cell can die rapidly under the influence of big shearing force.

There is a need for a high capacity suspension cell culture flask that ameliorates the above problems.

Disclosure of Invention

The invention aims to provide a high-capacity suspension cell culture bottle to solve the problems in the background technology.

In order to achieve the purpose, the invention provides the following technical scheme:

the utility model provides a large capacity suspension cell culture bottle, includes the blake bottle body, the upside internally mounted of blake bottle body has the upper portion air permeable membrane body, the ventilated membrane hole that is the circumference and distributes is seted up to the base plane of blake bottle body, department installs totally closed bottle lid subassembly in the middle of the upside of blake bottle body, the lower part air permeable membrane body is installed to the downside inner wall of blake bottle body, the blake bottom is installed to the bottom of blake bottle body.

According to a preferable scheme of the invention, the fully-closed bottle cap assembly comprises a culture bottle cap, a sealing washer, an upper connecting pipe, a lower connecting pipe and a connector, wherein the sealing washer is installed inside the culture bottle cap, the upper connecting pipe is installed on the fully-closed bottle cap assembly, the lower connecting pipe is installed at the lower end part of the upper connecting pipe and positioned at the lower side of the culture bottle cap, and the connector is installed at the upper end part of the upper connecting pipe.

As a preferable scheme of the present invention, the diameters of the upper connection tube and the lower connection tube are the same, and the upper connection tube and the lower connection tube may have a suitable length, size and number as required, and the connectors may be closed luer connectors, and the connectors may have a suitable number as required.

As a preferable scheme of the invention, the culture bottle body can be optimized into any suitable size and shape according to requirements, the connection mode between the culture bottle body and the fully-closed bottle cap assembly is threaded connection, and scale lines are etched on the outer wall of the culture bottle body.

In a preferred embodiment of the present invention, the upper connection pipe and the lower connection pipe may be connected to different types of pump bodies such as a peristaltic pump through a joint.

In a preferred embodiment of the present invention, the bottom cover of the culture bottle is provided with a bottom support boss array, and the bottom of the bottom cover of the culture bottle is provided with through holes distributed circumferentially.

As a preferable aspect of the present invention, the upper and lower breathable film bodies may be designed to have any shape and be attached to any suitable position according to requirements, and the upper and lower breathable film bodies may be designed to have any suitable shape and size according to requirements.

Compared with the prior art, the invention has the beneficial effects that:

1. according to the invention, when the culture bottle body is high in capacity and high in density, high survival rate and effective amplification speed of cell culture are realized, cell static culture and vibration culture are simultaneously satisfied, process flexibility of different cell types is satisfied, the gas flux multiple level of the device is increased by the upper gas-permeable membrane body and the lower gas-permeable membrane body compared with that of a traditional culture bottle, the upper surface and the lower surface of the culture bottle body are ensured to have gas-permeable functions, effective growth of suspended cells after sedimentation is satisfied, and internal and external pressure balance of the bottle body is realized.

2. In the invention, the upper connecting pipe, the lower connecting pipe and the joint of the fully-closed bottle cap component form a closed pipeline, so that the device can realize the fully-closed operation of liquid supplement (change), sampling and harvesting, meets the GMP production requirement, simultaneously provides a closed connecting pipe joint, can be butted with a semi-automatic unit and automatic equipment, can directly remove supernatant after the device is statically placed, is convenient for liquid supplement and liquid change operation, eliminates the complicated operations of centrifugal liquid change and the like in the process of the traditional culture bottle, and is designed as an integrated culture bottle as a whole, thereby reducing the risk of cross contamination.

Drawings

FIG. 1 is a schematic view of the overall structure of the present invention;

FIG. 2 is a schematic view of the internal structure of the flask body of the culture flask according to the present invention;

fig. 3 is a schematic structural view of the fully closed bottle cap assembly of the present invention.

In the figure: 1. a culture bottle body; 2. an upper breathable film body; 3. a gas permeable membrane pore; 4. a fully closed bottle cap assembly; 5. a lower breathable film body; 6. a bottom cover of the culture bottle; 7. a culture bottle cap; 8. a sealing gasket; 9. an upper connecting pipe; 10. a lower connecting pipe; 11. and (4) a joint.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, rather than all embodiments, and all other embodiments obtained by a person of ordinary skill in the art without any creative work based on the embodiments of the present invention belong to the protection scope of the present invention.

While several embodiments of the present invention will be described more fully hereinafter with reference to the accompanying drawings, in order to facilitate an understanding of the invention, the invention may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed to provide a more complete disclosure of the invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present, that when an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present, and that the terms "vertical", "horizontal", "left", "right" and the like are used herein for descriptive purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs, and the terms used herein in the specification of the present invention are for the purpose of describing particular embodiments only and are not intended to limit the present invention, and the term "and/or" as used herein includes any and all combinations of one or more of the associated listed items.

Referring to fig. 1-3, the present invention provides a technical solution:

the utility model provides a large capacity suspension cell culture bottle, includes blake bottle body 1, and blake bottle body 1's upside internally mounted has upper portion ventilative membrance body 2, and the ventilative membrane hole 3 that is the circumference and distributes is seted up to blake bottle body 1's base plane, and totally closed bottle lid subassembly 4 is installed to blake bottle body 1's upside middle department, and lower part ventilative membrance body 5 is installed to blake bottle body 1's downside inner wall, and blake bottle bottom 6 is installed to blake bottle body 1's bottom.

In the embodiment, referring to fig. 1-3, the fully-enclosed bottle cap assembly 4 includes a culture bottle cap 7, a sealing washer 8, an upper connecting pipe 9, a lower connecting pipe 10 and a joint 11, the sealing washer 8 is installed inside the culture bottle cap 7, the upper connecting pipe 9 is installed on the fully-enclosed bottle cap assembly 4, the lower connecting pipe 10 is installed at the lower end of the upper connecting pipe 9 and at the lower side of the culture bottle cap 7, the joint 11 is installed at the upper end of the upper connecting pipe 9, the diameters of the upper connecting pipe 9 and the lower connecting pipe 10 are the same, the upper connecting pipe 9 and the lower connecting pipe 10 can be selected to have proper lengths, sizes and numbers as required, the joint 11 is a closed luer joint, the joint 11 can be selected to have proper numbers as required, and the upper connecting pipe 9 and the lower connecting pipe 10 can be connected with different pump bodies such as a peristaltic pump through the joint 11, through the upper portion connecting pipe 9 of the totally closed bottle lid subassembly 4 that sets up, lower part connecting pipe 10 and joint 11 constitute the closed pipeline, make this device can realize mending (trade) liquid, take a sample, the totally enclosed operation of results, satisfy GMP production needs, provide simultaneously and seal the connecting pipe joint, can dock with semi-automatization unit and automation equipment, also make this device stand the back, can directly get rid of the supernatant, make things convenient for the liquid supplementation and trade the liquid operation, eliminate traditional blake bottle and trade operations such as liquid at loaded down with trivial details centrifugation in the process, this device formula blake bottle design as an organic whole is said, reduce cross contamination risk, and this device can realize ordinary bottle lid and the totally enclosed connecting pipe bottle lid and can change each other, can satisfy static culture and vibration culture simultaneously, have nimble technology matching nature, can satisfy the static culture of immunocyte.

In the embodiment, referring to fig. 1 and 2, a bottom support boss array is arranged on a bottom cover 6 of a culture bottle, through holes are circumferentially distributed on the lower side of the bottom cover 6 of the culture bottle, a culture bottle body 1 can be optimized to have liquid capacities of any suitable size, 1000ml, 2000ml, 5000ml or other specifications and shapes according to requirements, the connection mode between the culture bottle body 1 and a fully-closed bottle cap assembly 4 is a threaded connection, scale lines are etched on the outer wall of the culture bottle body 1, an upper air-permeable membrane body 2 and a lower air-permeable membrane body 5 can be designed to be attached to any suitable positions according to requirements in any shapes, the upper air-permeable membrane body 2 and the lower air-permeable membrane body 5 can be designed to have any suitable shapes and sizes according to requirements, when the culture bottle body 1 is large in capacity and high in density, high cell culture survival rate and effective amplification speed are realized, and cell static culture and vibration culture are simultaneously satisfied, satisfy different cell type process flexibility, the gas flux multiple level of this device increases with lower part gas permeable membrane body 5 in upper portion gas permeable membrane body 2 compared with traditional blake bottle, guarantees that the upper and lower two sides of blake bottle body 1 all has ventilation function, satisfies the effective growth after the suspension cell subsides, realizes the inside and outside pressure balance of bottle simultaneously.

The working process of the invention is as follows: putting a proper amount of cells and a culture medium into a culture bottle body 1, screwing a common culture bottle cap 7, performing static culture or shaking culture in an incubator, in the cell culture process, replacing the culture medium without adding the culture medium again, harvesting the grown cells or the products in the supernatant after a period of time, when performing another application, putting a proper amount of cells and the culture medium into the culture bottle body 1, inserting an upper connecting pipe 9 and a lower connecting pipe 10 into the culture bottle cap 7, sampling at any time through a joint 11 at the end part of the upper connecting pipe 9, directly discharging the upper supernatant after the cells are effectively settled in the culture process, performing liquid replacement and liquid supplementation operation, when harvesting the cells, extracting the supernatant through the upper connecting pipe 9, the lower connecting pipe 10 and the joint 11, then extracting the cells to perform cell collection operation without opening the culture bottle cap 7, avoiding pollution, in the process of shaking culture, cells are suspended in a culture solution, the gas exchange efficiency formed by direct contact of the lower gas-permeable membrane body 5 and the cells is reduced, the whole gas exchange flux is compensated by increasing the gas exchange flux on the upper surface of liquid, the total culture amount is effectively ensured, the bottle body 1 of the culture bottle can ensure the effective formation of vortex in the process of shaking culture, the upper connecting pipe 9, the lower connecting pipe 10 and the joint 11 of the totally-closed bottle cap component 4 form a closed pipeline, so that the device can realize the completely closed operation of liquid supplement (replacement), sampling and harvesting, meet the GMP production requirement, simultaneously provide a closed connecting pipe joint, can be butted with a semi-automatic unit and automatic equipment, can directly remove supernatant after the device is placed statically, is convenient for liquid supplement and replacement operation, and eliminates the complicated operations of centrifugal liquid replacement and the like of the traditional culture bottle in the process, wholly speaking this device formula blake bottle design as an organic whole, reduce the cross contamination risk, and this device can realize that ordinary bottle lid and totally-enclosed connecting tube bottle lid can be changed each other, can satisfy static culture and vibration culture simultaneously, flexible technology matching nature has, can satisfy the static culture of immunocyte, blake bottle body 1 is when large capacity and high density, realize cell culture high rate of activity and effective rate of amplification, satisfy cell static culture and vibration culture simultaneously, satisfy different cell type technology flexibility, the gas flux multiple level increase of this device is compared with traditional blake bottle to upper portion gas-permeable membrane body 2 and lower part gas-permeable membrane body 5, guarantee that the upper and lower two sides of blake bottle body 1 all have ventilative function, satisfy the effective growth after the suspension cell subsides, realize inside and outside pressure balance of bottle simultaneously.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. The utility model provides a large capacity suspension cell culture bottle, includes culture bottle body (1), its characterized in that: the utility model discloses a culture flask, including the culture flask body, the upside internally mounted of culture flask body (1) has upper portion air permeable membrane body (2), air permeable membrane hole (3) that are the circumference and distribute are seted up to the base plane of culture flask body (1), department installs totally closed bottle lid subassembly (4) in the middle of the upside of culture flask body (1), lower part air permeable membrane body (5) are installed to the downside inner wall of culture flask body (1), culture flask bottom (6) are installed to the bottom of culture flask body (1).

2. A large capacity suspension cell culture flask as claimed in claim 1, wherein: totally closed bottle lid subassembly (4) include blake bottle lid (7), seal ring (8), upper portion connecting pipe (9), lower part connecting pipe (10) and joint (11), the internally mounted of blake bottle lid (7) has seal ring (8), install upper portion connecting pipe (9) on the totally closed bottle lid subassembly (4), lower part connecting pipe (10) are installed to the downside tip of upper portion connecting pipe (9) and the downside that is located blake bottle lid (7), joint (11) are installed to the upside tip of upper portion connecting pipe (9).

3. A large capacity suspension cell culture flask as claimed in claim 2, wherein: the diameters of the upper connecting pipe (9) and the lower connecting pipe (10) are the same, the upper connecting pipe (9) and the lower connecting pipe (10) can be selected to be proper in length, size and number according to needs, the joint (11) is a closed luer joint, and the joint (11) can be selected to be proper in number according to needs.

4. A large capacity suspension cell culture flask as claimed in claim 1, wherein: the culture bottle body (1) can be optimized into any suitable size (different liquid capacity, 1000ml, 2000ml, 5000ml or other specifications) and shape as required, the connection mode between the culture bottle body (1) and the fully-closed bottle cap component (4) is threaded connection, and the outer wall of the culture bottle body (1) is etched with scale marks.

5. A large capacity suspension cell culture flask as claimed in claim 3, wherein: the upper connecting pipe (9) and the lower connecting pipe (10) can be connected with different forms of pump bodies such as peristaltic pumps through a joint (11).

6. A large capacity suspension cell culture flask as claimed in claim 1, wherein: the bottom support boss array is arranged on the culture bottle bottom cover (6), and through holes distributed circumferentially are formed in the lower side of the culture bottle bottom cover (6).

7. A large capacity suspension cell culture flask as claimed in claim 1, wherein: the upper breathable film body (2) and the lower breathable film body (5) can be designed into any shapes and attached to any suitable positions according to requirements, and the upper breathable film body (2) and the lower breathable film body (5) can be designed into any suitable shapes and sizes according to requirements.