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CN206843507U - A kind of suspension cell culture device - Google Patents

A kind of suspension cell culture device Download PDF

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Publication number
CN206843507U
CN206843507U CN201720660285.0U CN201720660285U CN206843507U CN 206843507 U CN206843507 U CN 206843507U CN 201720660285 U CN201720660285 U CN 201720660285U CN 206843507 U CN206843507 U CN 206843507U
Authority
CN
China
Prior art keywords
bottle
hole
probe tube
air inlet
inlet pipe
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN201720660285.0U
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Chinese (zh)
Inventor
姚玲玲
杨敏
孟得龙
杨苗
宋丹
刘得娟
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Beijing Biotechnology Research Institute
Huanshenghui Biogenetic Technology (Beijing) Co., Ltd.
Original Assignee
Beijing Biotechnology Research Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
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Priority to CN201720660285.0U priority Critical patent/CN206843507U/en
Application granted granted Critical
Publication of CN206843507U publication Critical patent/CN206843507U/en
Active legal-status Critical Current
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Abstract

The utility model discloses a kind of suspension cell culture device, including bottle, centring ring, supporting pad, air inlet pipe, branch pipe, bottle cap, ventilated membrane, probe tube, thief hole, air bag ball, plug, connection is pasted between centring ring inwall and bottle outer surface, nested encryptions between centring ring outside and agitator, welded between supporting pad one end and bottle bottom surface, it is threadedly coupled between the bottle cap and bottle and through hole is offered on bottle cap, air inlet pipe stretches into bottle through through hole and connection is connected branch pipe one end and air inlet pipe, ventilated membrane is nested in air inlet pipe end and is fitted and connected between ventilated membrane and branch pipe surface, also through hole is offered on bottle and probe tube is stretched into bottle through through hole, connection is connected between probe tube top and air bag ball, thief hole is further opened with probe tube.This suspension cell culture device provides good oxygen exchange for cell by ventilated membrane, reduces fluid infusion operation, saves consumptive material, saves manpower, improve cell yield.

Description

A kind of suspension cell culture device
Technical field
It the utility model is related to cell culture apparatus, and in particular to a kind of suspension cell culture device.
Background technology
The device used on existing market on cell culture, all it is blake bottle or culture bag mostly, as on a large scale Production, blake bottle take up space greatly, and the liquid filled has limitation, in transportation, also needs great care, prevent that cell is dirty Dye, causes to waste;It is cumbersome, it is necessary to some auxiliary equipments using cell culture bags, such as:Liquid supply device, syringe etc., Wasting manpower and material resources.
Utility model content
The purpose of this utility model is to provide a kind of suspension cell culture device, to solve to propose in above-mentioned background technology The problem of.
To achieve the above object, the utility model provides following technical scheme:A kind of suspension cell culture device, including bottle Body, centring ring, supporting pad, air inlet pipe, branch pipe, bottle cap, ventilated membrane, probe tube, thief hole, air bag ball, plug, the centring ring Connection, nested encryptions between centring ring outside and agitator, described supporting pad one end are pasted between inwall and bottle outer surface Welded between bottle bottom surface, be threadedly coupled between the bottle cap and bottle and through hole is offered on bottle cap, the air inlet pipe Stretched into through through hole in bottle and connection is connected branch pipe one end and air inlet pipe, the ventilated membrane is nested in air inlet pipe end Hold and be fitted and connected between ventilated membrane and branch pipe surface, also offer through hole on the bottle and probe tube is stretched through through hole Enter in bottle, the plug is nested on probe tube and is carved with screw thread on the outside of plug, probe tube by plug and bottle it Between thread seal connect, connection is connected between probe tube top and air bag ball, thief hole, the sampling are further opened with probe tube Hole equidistantly distributed is in probe tube.
Preferably, the branch pipe is made using soft transparent plastic and equidistantly offers air-vent on branch pipe.
Preferably, the bottle is shaped as cylinder.
Compared with prior art, the beneficial effects of the utility model are:The ventilated membrane of this suspension cell culture device bottom, Good oxygen exchange is provided for cell, improves cell yield;Fluid infusion operation is reduced, consumptive material is saved, reduces cell culture Cost, save the use of culture medium and the factor, save space;The pattern of cylinder can carry the culture medium of more multicapacity, lead to Over sampling pipe can be more convenient to be sampled detection, and use is more convenient.
Brief description of the drawings
Fig. 1 is the utility model structure diagram.
Fig. 2 is the utility model probe tube schematic diagram.
In figure:1- bottles, 2- centring rings, 3- air inlet pipe, 4- branch pipes, 5- ventilated membranes, 6- supporting pads, 7- probe tubes, 8- take Sample hole, 9- air bag balls, 10- bottle caps, 11- plugs.
Embodiment
Below in conjunction with the accompanying drawing in the utility model embodiment, the technical scheme in the embodiment of the utility model is carried out Clearly and completely describing, it is clear that described embodiment is only the utility model part of the embodiment, rather than whole Embodiment.Based on the embodiment in the utility model, those of ordinary skill in the art are not under the premise of creative work is made The every other embodiment obtained, belong to the scope of the utility model protection.
Refer to Fig. 1-2, a kind of embodiment provided by the utility model:A kind of suspension cell culture device, including bottle 1st, centring ring 2, supporting pad 6, air inlet pipe 3, branch pipe 4, bottle cap 10, ventilated membrane 5, probe tube 7, thief hole 8, air bag ball 9, plug 11, connection, the outside of centring ring 2 company nested between agitator are pasted between the inwall of centring ring 2 and bottle 1 outer surface Connect, welded between described one end of supporting pad 6 and the bottom surface of bottle 1, is threadedly coupled between the bottle cap 10 and bottle 1 and bottle cap 10 On offer through hole, the air inlet pipe 3 is stretched into bottle 1 through through hole and connected the one end of branch pipe 4 and air inlet pipe 3 Connect, the ventilated membrane 5 is nested in the end of air inlet pipe 3 and is fitted and connected between ventilated membrane 5 and the surface of branch pipe 4, on the bottle 1 Also offer through hole and probe tube 7 is stretched into bottle 1 through through hole, the plug 11 is nested on probe tube 7 and glue Screw thread is carved with the outside of plug 11, and probe tube 7 is connected by thread seal between plug 11 and bottle 1, the top of probe tube 7 and air bag ball Connection is connected between 9, is further opened with thief hole 8 on probe tube 7, the equidistantly distributed of thief hole 8 is in probe tube 7, branch pipe 4 It is made using soft transparent plastic and air-vent is equidistantly offered on branch pipe 4, bottle 1 is shaped as cylinder.
Specifically used mode:In the utility model, pass through bottle 1, centring ring 2, supporting pad 6, air inlet pipe 3, branch pipe 4, bottle Lid 10, ventilated membrane 5, probe tube 7, thief hole 8, air bag ball 9, plug 11 constitute the present apparatus, and spiral shell corkage lid 10 is by branch pipe 4 and thoroughly Air film 5 is put into bottle 1, is then poured into culture medium in bottle 1, and bottle 1 is communicated by air inlet pipe 3 with the external world, air inlet pipe 3 with Connected between branch pipe 4, oxygen exchange can be carried out by the cell of ventilated membrane 5, can be placed on agitator and carried out by centring ring 2 Stirring, when thinking sampling detection, with hand by pinching air bag ball 9, by probe tube 7 by cell extraction into air bag ball 9, wherein sampling Hole 8 can collect the cell sample of different height, and sampling is uniform.
It is obvious to a person skilled in the art that the utility model is not limited to the details of above-mentioned one exemplary embodiment, and And in the case of without departing substantially from spirit or essential attributes of the present utility model, it can realize that this practicality is new in other specific forms Type.Therefore, no matter from the point of view of which point, embodiment all should be regarded as exemplary, and is nonrestrictive, this practicality is new The scope of type limits by appended claims rather than described above, it is intended that the equivalency fallen in claim is contained All changes in justice and scope are included in the utility model.Any reference in claim should not be considered as limitation Involved claim.
Moreover, it will be appreciated that although the present specification is described in terms of embodiments, not each embodiment is only wrapped Containing an independent technical scheme, this narrating mode of specification is only that those skilled in the art should for clarity Using specification as an entirety, the technical solutions in the various embodiments may also be suitably combined, forms those skilled in the art It is appreciated that other embodiment.

Claims (3)

1. a kind of suspension cell culture device, including bottle (1), centring ring (2), supporting pad (6), air inlet pipe (3), branch pipe (4), Bottle cap (10), ventilated membrane (5), probe tube (7), thief hole (8), air bag ball (9), plug (11), it is characterised in that:The positioning Enclose to paste between (2) inwall and bottle (1) outer surface and connect, nested encryptions between centring ring (2) outside and agitator are described Weld, be threadedly coupled between the bottle cap (10) and bottle (1) and bottle cap between supporting pad (6) one end and bottle (1) bottom surface (10) through hole is offered on, the air inlet pipe (3) is stretched into through through hole in bottle (1) and branch pipe (4) one end and air inlet pipe (3) connect connection between, the ventilated membrane (5) be nested in air inlet pipe (3) end and ventilated membrane (5) and branch pipe (4) surface it Between be fitted and connected, through hole is also offered on the bottle (1) and probe tube (7) is stretched into bottle (1) through through hole, it is described Plug (11) is nested on probe tube (7) and is carved with screw thread on the outside of plug (11), and probe tube (7) passes through plug (11) and bottle (1) thread seal connects between, connection is connected between probe tube (7) top and air bag ball (9), probe tube is further opened with (7) Thief hole (8), thief hole (8) equidistantly distributed is in probe tube (7).
2. a kind of suspension cell culture device according to claim 1, it is characterised in that the branch pipe (4) uses software Transparent plastic material is made and branch pipe (4) on equidistantly offer air-vent.
3. a kind of suspension cell culture device according to claim 1, it is characterised in that bottle (1) is shaped as Cylinder.
CN201720660285.0U 2017-06-08 2017-06-08 A kind of suspension cell culture device Active CN206843507U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201720660285.0U CN206843507U (en) 2017-06-08 2017-06-08 A kind of suspension cell culture device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201720660285.0U CN206843507U (en) 2017-06-08 2017-06-08 A kind of suspension cell culture device

Publications (1)

Publication Number Publication Date
CN206843507U true CN206843507U (en) 2018-01-05

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Family Applications (1)

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CN201720660285.0U Active CN206843507U (en) 2017-06-08 2017-06-08 A kind of suspension cell culture device

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CN (1) CN206843507U (en)

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110656048A (en) * 2019-11-14 2020-01-07 广州瑞铂茵健康管理咨询有限公司 Cell culture cell and have its culture vessel
CN113462570A (en) * 2021-07-12 2021-10-01 英诺维尔智能科技(苏州)有限公司 High-capacity suspension cell culture bottle

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN110656048A (en) * 2019-11-14 2020-01-07 广州瑞铂茵健康管理咨询有限公司 Cell culture cell and have its culture vessel
CN113462570A (en) * 2021-07-12 2021-10-01 英诺维尔智能科技(苏州)有限公司 High-capacity suspension cell culture bottle
CN113462570B (en) * 2021-07-12 2024-02-09 英诺维尔智能科技(苏州)有限公司 High-capacity suspension cell culture bottle

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GR01 Patent grant
GR01 Patent grant
TR01 Transfer of patent right

Effective date of registration: 20190613

Address after: Room 1006, 10th floor, No. 3 Courtyard, 30 Shixing Street, Shijingshan District, Beijing, 100041

Co-patentee after: Beijing Biotechnology Research Institute

Patentee after: Huanshenghui Biogenetic Technology (Beijing) Co., Ltd.

Address before: Room 1002, 10th floor, 3rd building, 30 Shixing Street, Shijingshan District, Beijing

Patentee before: Beijing Biotechnology Research Institute

TR01 Transfer of patent right