CN221662955U - Cell culture apparatus - Google Patents

Abstract

The embodiment of the application provides a cell incubator. The cell culture apparatus comprises a bottle body and an observation cavity, wherein the bottle body is provided with a first accommodating space, and comprises a side wall and a bottom wall; the observation cavity is provided with a second accommodating space, is arranged on the outer side of the bottle body in the radial plane of the bottle body and is connected with the bottle body, and the second accommodating space is communicated with the first accommodating space through a communication port; the observation cavity comprises a first wall and a second wall which are arranged in parallel and is used for observing cells; the first wall and the second wall are both connected to the side wall and are both parallel to the bottom wall. The embodiment of the application can realize cell observation in the cell culture process and reduce pollution risk.

Description

Cell culture apparatus

Technical Field

The application relates to the technical field of cell culture, in particular to a cell culture device.

Background

Suspension cells can be serially subcultured in the same cell culture apparatus, and the subculturing standard is mainly judged according to the cell state and the cell concentration. When the suspension cell density reaches the maximum growth density, the cells will stop growing and even die.

Some cell culture apparatus cannot be observed under a microscope, cell death due to excessive cell growth is likely to occur, and daily observation of cells is not possible. If the culture solution in the cell culture apparatus is taken out for observation, the number of times of uncovering operation is increased, pollution risk exists, and the sterile production standard is difficult to meet.

Disclosure of utility model

Aiming at the defects of the related technology, the application provides a cell culture device which is used for solving the technical problem that the pollution risk possibly exists when a culture solution is taken out for observation in the cell culture process in the related technology.

In a first aspect, embodiments of the present application provide a cell culture apparatus comprising:

The bottle body is provided with a first accommodating space and comprises a side wall and a bottom wall;

The observation cavity is provided with a second accommodating space, is arranged on the outer side of the bottle body in the radial plane of the bottle body and is connected with the bottle body, and the second accommodating space is communicated with the first accommodating space through a communication port; the observation cavity comprises a first wall and a second wall which are arranged in parallel and is used for observing cells; the first wall and the second wall are both connected to the side wall and are both parallel to the bottom wall.

In one possible implementation, the first wall and the second wall are provided at an end of the side wall near the bottom wall.

In one possible implementation, an end of the side wall remote from the bottom wall forms a bottle mouth; the first wall and the bottom wall are positioned in the same plane, and the second wall is arranged on one side of the first wall, which is close to the bottle mouth.

In one possible implementation, the viewing chamber further comprises a connecting wall connecting the first wall and the second wall, the connecting wall being perpendicular to both the first wall and the second wall;

The connecting wall is connected with the side wall and encloses the communication port together with the first wall and the second wall.

In one possible implementation, the body and the viewing cavity are aligned along a first direction;

The viewing chamber has a constant width in a plane parallel to the bottom wall and perpendicular to the first direction; or in a direction away from the side wall, the viewing chamber tapers in width in a plane parallel to the bottom wall and perpendicular to the first direction.

In one possible implementation, the overlapping region of the first wall and the second wall forms a viewing zone, the area of which meets the requirements of the design cell experiment and matches the space under the microscope.

In one possible implementation, the body and the viewing cavity are aligned along a first direction;

the viewing cavity has a length in the first direction that is greater than a width of the viewing cavity in a plane parallel to the bottom wall and perpendicular to the first direction.

In one possible implementation, the thickness range of the observation cavity in the direction perpendicular to the first wall meets the flow requirement of the culture solution and is matched with the space below the microscope.

In one possible implementation, the body includes a side wall and a bottom wall, the side wall including a main body region and a first region remote from the bottom wall;

The maximum static friction of the first region is greater than the maximum static friction of the body region; and/or the main body area is provided with capacity graduation marks.

The technical scheme provided by the embodiment of the application has the beneficial technical effects that:

The cell culture device comprises a bottle body and an observation cavity, wherein the bottle body is provided with a first accommodating space; the observation cavity is provided with a second accommodating space, is arranged on the outer side of the bottle body in the radial plane of the bottle body and is connected with the bottle body, and the second accommodating space is communicated with the first accommodating space through a communication port; the observation cavity comprises a first wall and a second wall which are arranged in parallel and used for observing cells, and the first wall and the second wall of the observation cavity are connected with the side wall of the bottle body and are parallel to the bottom wall of the bottle body. Through add the observation chamber with the body intercommunication in the radial plane of body, observe the chamber including parallel arrangement's first wall and second wall, the effect of first wall and second wall is similar to upper and lower two slide, is convenient for place the observation chamber in the microscope and observes the condition such as form, density and the growth state of cell, also the daily observation of cell of being convenient for, can reduce the pollution risk that the cell culture in-process takes out the culture solution and observes and cause.

Additional aspects and advantages of the application will be set forth in part in the description which follows, and in part will be obvious from the description, or may be learned by practice of the application.

Drawings

The foregoing and/or additional aspects and advantages of the application will become apparent and readily appreciated from the following description of the embodiments, taken in conjunction with the accompanying drawings, in which:

FIG. 1 is a front view of a cell culture apparatus according to an embodiment of the application;

FIG. 2 is a top view of a cell incubator according to an embodiment of the present application;

FIG. 3 is a top view of another cell culture apparatus according to an embodiment of the application.

Description of the reference numerals:

100-cell culture apparatus;

11-a bottle body;

111-sidewalls; 111 a-a first region; 112-a bottom wall;

12-an observation cavity;

121-a first wall; 122-a second wall; 123-connecting walls;

13-bottle cap;

131-ventilation film holes;

L1-width;

L2-length;

h-thickness;

X-first direction.

Detailed Description

Embodiments of the present application are described below with reference to the drawings in the present application. It should be understood that the embodiments described below with reference to the drawings are exemplary descriptions for explaining the technical solutions of the embodiments of the present application, and the technical solutions of the embodiments of the present application are not limited.

For the purpose of making the objects, technical solutions and advantages of the present application more apparent, the embodiments of the present application will be described in further detail with reference to the accompanying drawings.

Some cell culture apparatus cannot be observed under a microscope, cell death due to excessive cell growth is likely to occur, and daily observation of cells is not possible. If the culture solution in the cell culture apparatus is taken out for observation, the number of times of uncovering operation is increased, pollution risk exists, and the sterile production standard is difficult to meet.

The application provides a cell culture device, which aims to solve the technical problems in the related art.

The following describes the technical scheme of the present application and how the technical scheme of the present application solves the above technical problems in detail with specific embodiments. It should be noted that the following embodiments may be referred to, or combined with each other, and the description will not be repeated for the same terms, similar features, similar implementation steps, and the like in different embodiments.

An embodiment of the present application provides a cell culture apparatus 100, wherein a schematic structure of the cell culture apparatus 100 is shown in fig. 1 to 3, and the cell culture apparatus comprises: a body 11 and a viewing chamber 12.

The body 11 has a first receiving space, and the body 11 may include a side wall 111 and a bottom wall 112.

The observation cavity 12 is provided with a second accommodation space, and the observation cavity 12 is arranged on the outer side of the bottle body 11 in the radial plane of the bottle body 11 and is connected with the bottle body 11, and the second accommodation space is communicated with the first accommodation space through a communication port; the viewing chamber 12 comprises a first wall 121 and a second wall 122 arranged in parallel for viewing cells; the first wall 121 and the second wall 122 are each connected to the side wall 111 and are each parallel to the bottom wall 112.

Through add the observation chamber 12 with the radial plane of body 11 intercommunication, observation chamber 12 includes parallel arrangement's first wall 121 and second wall 122, and the effect of first wall 121 and second wall 122 is similar to two upper and lower slides, is convenient for place the observation chamber 12 under the microscope and observes the condition such as form, density and the growth state of cell, also the daily observation of cell of being convenient for, can reduce the pollution risk that the cell culture in-process takes out the culture solution and observes and cause.

Since the observation cavity 12 is disposed on the side wall 111, and the first wall 121 and the second wall 122 are parallel to the bottom wall 112, the stability of the cell culture apparatus 100 can be prevented from being affected, and the observation cavity 12 can be stably placed under a microscope for cell observation.

Referring to fig. 1, alternatively, the first wall 121 and the second wall 122 may be provided at an end of the side wall 111 near the bottom wall 112.

Since the observation cavity 12 is disposed at a position of the side wall 111 of the bottle body 11 near the bottom wall 112, the center of gravity of the whole cell culture apparatus 100 is lowered, which is beneficial to the stability of the cell culture apparatus 100.

Referring to fig. 1, optionally, in some embodiments of the application, an end of the side wall 111 remote from the bottom wall 112 forms a bottle opening; the first wall 121 and the bottom wall 112 are located in the same plane, and the second wall 122 is disposed on the side of the first wall 121 close to the bottle mouth.

Since the bottom of the observation chamber 12 is flush with the bottom wall 112 of the bottle body 11, the problem of influencing the observation result due to insufficient culture medium flowing into the observation chamber 12 can be avoided.

Of course, in other possible embodiments, the first wall 121 and the second wall 122 may be disposed at an end of the side wall 111 away from the bottom wall 112, or at an intermediate position of the side wall 111, which is not limited herein.

Specifically, in some embodiments of the present application, sidewall 111 may be gradually drawn inwardly in the direction of bottom wall 112 toward the mouth of the bottle and form the mouth of the bottle at one end, and cell culture apparatus 100 further comprises cap 13.

Referring to fig. 2 and 3, optionally, the bottle cap 13 is provided with ventilation film holes 131 distributed circumferentially, and a ventilation film permeable to gas and impermeable to liquid may be attached to the inside of the bottle cap 13.

In other words, the body 11 of the cell culture apparatus 100 may resemble a flask. The triangular shake flask has the advantages of difficult overflow, uniform strength and better gas exchange efficiency, can remarkably improve the culture efficiency and shortens the production period.

The cell culture apparatus 100 provided in some embodiments of the present application may be used for mass culture of suspended cells, and also for large-scale fermentation processes and the like. Because the cell culture apparatus 100 of the embodiment of the present application has a relatively large culture volume, it is very advantageous for collection of the culture in addition to the large-scale culture, and the situation that a large amount of liquid in the culture apparatus is required for the conventional culture apparatus is avoided.

Referring to fig. 1, in some embodiments of the application, viewing chamber 12 may further include a connecting wall 123 connecting first wall 121 and second wall 122, connecting wall 123 being perpendicular to both first wall 121 and second wall 122. The connecting wall 123 is connected to the side wall 111 and encloses a communication port together with the first wall 121 and the second wall 122.

In other words, the communication port in some embodiments of the present application has a rectangular shape projected in a plane perpendicular to the first wall 121. In practical applications, the projection of the communication port in the plane perpendicular to the first wall 121 may be circular, trapezoidal (the upper and lower bases of the trapezoid are parallel to the first wall 121), etc., which is not limited herein.

Referring to fig. 1 through 3, in some embodiments of the present application, the body 11 and viewing chamber 12 are aligned in a first direction.

Referring to fig. 2, in some embodiments of the application, the viewing chamber 12 has a constant width L1 in a plane parallel to the bottom wall 112 and perpendicular to the first direction X.

In other words, the viewing chamber 12 resembles a cuboid and the viewing area resembles a rectangle. By the arrangement, the culture solution can smoothly flow into the observation cavity 12, and interference between the microscope lens and the bottle body 11 when the observation cavity 12 stretches into the space below the microscope can be avoided.

Referring to fig. 3, in alternative possible embodiments of the application, the viewing chamber 12 tapers in width L1 in a plane parallel to the bottom wall 112 and perpendicular to the first direction X, in a direction away from the side wall 111.

In other words, the viewing chamber 12 resembles the mouth of a necked bottle (i.e., the mouth of an inverted open bottle) and the viewing area resembles a trapezoid. The arrangement is such that the communication port is larger, which is beneficial for the culture solution to smoothly flow into the observation cavity 12.

The viewing chamber 12 may have a ring shape or a partial ring shape, and the like, and is not limited thereto.

Referring to fig. 1, in some embodiments of the present application, the overlapping region of the first wall 121 and the second wall 122 forms a viewing zone, the area of which meets the requirements of the designed cell experiment and matches the space under the microscope.

The arrangement can ensure that the observation area can meet the requirement of cell experiments, and can meet the requirement that the observation cavity 12 is easily arranged under the microscope.

By way of example, the area of the viewing area may range from 25 square centimeters to 35 square centimeters. For example, 25 square centimeters, 30 square centimeters, 35 square centimeters, etc.

Referring to fig. 2, in alternative embodiments of the application, the length L2 of the viewing chamber 12 in the first direction X is greater than the width L1 of the viewing chamber 12 in a plane parallel to the bottom wall 112 and perpendicular to the first direction X.

Since the length L2 of the observation cavity 12 is larger than the width L1, that is, the observation cavity 12 is elongated, interference between the microscope lens and the bottle body 11 when the observation cavity 12 extends into the space below the microscope can be avoided.

By way of example, the length L2 of the viewing chamber 12 in the first direction X ranges from 65 millimeters to 75 millimeters, e.g., 65 millimeters, 70 millimeters, 75 millimeters, etc. The viewing chamber 12 has a width L1 in a plane parallel to the bottom wall 112 and perpendicular to the first direction X in the range 40 mm to 50 mm, e.g., 40 mm, 45 mm, 50 mm, etc.

Of course, in practical applications, the length L2 of the observation cavity 12 may be smaller than the width L1, or the length L2 of the observation cavity 12 may be equal to the width L1, which is not limited herein.

Referring to fig. 1, in some embodiments of the present application, the thickness H of the observation chamber 12 in the direction perpendicular to the first wall 121 may be selected to meet the flow requirements of the culture medium and match the space under the microscope.

So set up, not only can avoid leading to the liquid flow to be hindered because of thickness H is too low, and then be insufficient for reflecting the problem of the overall state and the density of cells in the blake bottle, can also avoid leading to being difficult to place the observation chamber 12 in the problem of microscope space below because of thickness H is too big.

By way of example, the viewing chamber 12 may have a thickness H in a direction perpendicular to the first wall 121 in the range of 28 mm to 30 mm, e.g., 28 mm, 29 mm, 30 mm, etc.

If the viewing chamber 12 is shorter in length or narrower in width, the viewing area may not reflect the overall condition of the cell, resulting in failure to meet the experimental requirements for viewing the cell under a microscope; if the thickness, length and width of the observation cavity 12 are too large, the convenience of use is reduced, and through the design of the size of the observation cavity 12, the experimental requirements of observing cells under a microscope can be met, and the observation cavity has better convenience of use.

Referring to fig. 1, in particular, in some embodiments of the application, the sidewall 111 includes a body region and a first region 111a remote from the bottom wall 112.

Alternatively, the maximum static friction of the first region 111a of the body 11 may be greater than the maximum static friction of the body region.

For example, a plurality of protrusions or grooves may be added to the first region 111a (i.e., at the bottleneck) to increase the anti-slip properties of the first region 111a to facilitate gripping of the cell culture apparatus 100 and prevent the cell culture apparatus 100 from slipping out and causing loss.

Alternatively, the body region of the body 11 may be provided with volume graduations.

The arrangement facilitates observation of the culture volume in the cell culture apparatus 100, and facilitates the fluid replacement operation during cell culture such as suspension cells.

The cell culture apparatus 100 according to the embodiment of the application adopts a sterilized single package, and is disposable, and the outside of the cell culture apparatus 100 is provided with an anti-collision filler and a packing box to prevent the cell culture apparatus 100 from being broken. The transport and storage temperature of the cell culture apparatus 100 is at normal temperature.

The technical scheme provided by the embodiment of the application has the beneficial technical effects that:

1. Through add the observation chamber 12 with the radial plane of body 11 intercommunication, observation chamber 12 includes parallel arrangement's first wall 121 and second wall 122, and the effect of first wall 121 and second wall 122 is similar to two upper and lower slides, is convenient for place the observation chamber 12 under the microscope and observes the condition such as form, density and the growth state of cell, also the daily observation of cell of being convenient for, can reduce the pollution risk that the cell culture in-process takes out the culture solution and observes and cause.

2. Since the observation cavity 12 is longer than it is wide, that is, the observation cavity 12 is long and narrow, interference between the microscope lens and the bottle body 11 when the observation cavity 12 extends into the space below the microscope can be avoided.

3. A large number of protrusions or recesses are added at the bottleneck to increase the anti-slip property of the first region 111a, so as to facilitate the grasping of the cell culture apparatus 100 and prevent the cell culture apparatus 100 from slipping down to cause loss.

4. The main body region of the bottle body 11 can be provided with capacity graduation marks, so that the culture volume in the cell culture device 100 can be observed conveniently, and the liquid supplementing operation during cell culture of suspended cells and the like is facilitated.

The foregoing is only a part of the embodiments of the present application, and it should be noted that, for those skilled in the art, other similar implementation means based on the technical idea of the present application may be adopted without departing from the technical idea of the solution of the present application, which is also within the protection scope of the embodiments of the present application.

Claims (9)

1. A cell culture apparatus, comprising:

a body (11) having a first accommodation space, the body (11) including a side wall (111) and a bottom wall (112);

An observation cavity (12) which is provided with a second accommodation space, is arranged on the outer side of the bottle body in the radial plane of the bottle body and is connected with the bottle body, and the second accommodation space is communicated with the first accommodation space through a communication port; the viewing chamber comprises a first wall (121) and a second wall (122) arranged in parallel for viewing cells; the first wall (121) and the second wall (122) are both connected to the side wall (111) and are both parallel to the bottom wall (112).

2. The cell culture vessel according to claim 1, wherein the first wall (121) and the second wall (122) are arranged at an end of the side wall (111) near the bottom wall (112).

3. Cell culture vessel according to claim 2, characterized in that the end of the side wall (111) remote from the bottom wall (112) forms a bottle mouth; the first wall (121) and the bottom wall (112) are located in the same plane, and the second wall (122) is arranged on one side, close to the bottle mouth, of the first wall (121).

4. The cell culture vessel according to claim 1, wherein the viewing chamber (12) further comprises a connecting wall (123) connecting the first wall (121) and the second wall (122), the connecting wall (123) being perpendicular to both the first wall (121) and the second wall (122);

the connecting wall (123) is connected with the side wall (111) and encloses the communication port together with the first wall (121) and the second wall (122).

5. Cell culture device according to claim 4, characterized in that said body (11) and said viewing chamber (12) are arranged along a first direction;

-the viewing cavity (12) has a constant width in a plane parallel to the bottom wall (112) and perpendicular to the first direction; or in a direction away from the side wall (111), the viewing chamber (12) tapers in width in a plane parallel to the bottom wall (112) and perpendicular to the first direction.

6. The cell culture apparatus according to claim 4, wherein the overlapping area of the first wall (121) and the second wall (122) forms a viewing zone, the area of which is in a range that meets the requirements of the designed cell experiment and matches the space under the microscope.

7. The cell culture apparatus according to claim 6, wherein the body (11) and the viewing chamber (12) are arranged in a first direction;

The viewing cavity (12) has a length in the first direction that is greater than a width of the viewing cavity (12) in a plane parallel to the bottom wall (112) and perpendicular to the first direction.

8. The cell culture apparatus according to claim 4, wherein the thickness of the viewing chamber (12) in a direction perpendicular to the first wall (121) is in a range that meets the flow requirements of the culture medium and matches the space under the microscope.

9. The cell culture vessel according to claim 1, wherein the body (11) comprises a side wall (111) and a bottom wall (112), the side wall (111) comprising a main body region and a first region (111 a) remote from the bottom wall (112);

The maximum static friction of the first region (111 a) is greater than the maximum static friction of the body region; and/or the main body area is provided with capacity graduation marks.