CN113354474A - Agricultural and forestry crop foliar bacterial liquid and preparation method thereof - Google Patents
Agricultural and forestry crop foliar bacterial liquid and preparation method thereof Download PDFInfo
- Publication number
- CN113354474A CN113354474A CN202110794471.4A CN202110794471A CN113354474A CN 113354474 A CN113354474 A CN 113354474A CN 202110794471 A CN202110794471 A CN 202110794471A CN 113354474 A CN113354474 A CN 113354474A
- Authority
- CN
- China
- Prior art keywords
- bacterial liquid
- agricultural
- stirring
- fermentation
- organic fertilizer
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05C—NITROGENOUS FERTILISERS
- C05C11/00—Other nitrogenous fertilisers
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Plant Pathology (AREA)
- Fertilizers (AREA)
Abstract
The invention relates to an agricultural and forestry crop foliar bacterial liquid and a preparation method thereof, wherein the agricultural and forestry crop foliar bacterial liquid is prepared by taking a bio-organic fertilizer leachate as a morphological matrix of the agricultural and forestry crop foliar bacterial liquid, taking chitosan oligosaccharide as a core substance of a plant vaccine, adding hydrolyzed feather compound amino acid and compound probiotic bacterial liquid for mixing and compatibility, and finally preparing the agricultural and forestry crop foliar bacterial liquid, under the action of the chitosan oligosaccharide, so that the effects of the bio-organic fertilizer leachate, the hydrolyzed feather compound amino acid and the compound probiotic bacterial liquid can be synergistically exerted, and the synergistic effects of supplementing each other, enhancing immunity and solving the source are achieved in the aspects of promoting plant growth, inhibiting harmful bacteria, improving the yield of single-column fruit thinning, improving the quality of fruits and vegetables, reducing pesticide and heavy metal residues and the like. The agricultural and forestry crop foliar bacterial liquid provides possibility for ecological green planting, greatly reduces the use amount of pesticides and chemical fertilizers, and promotes the development of agricultural planting to the aspects of ecological cycle and green safety.
Description
Technical Field
The invention belongs to the technical field of leaf fertilizers, and particularly relates to an agricultural and forestry crop leaf surface bacterial liquid and a preparation method thereof.
Background
China is a big agricultural country, and the amount of fertilizer used is the first in the world every year, and can reach more than 1 hundred million tons. The birth of the chemical fertilizer breaks through the traditional agricultural fertilization mode in China, creates a new era of agricultural fertilizer, makes great contribution to agricultural production increase and income increase of farmers for a long time, but causes the soil structure damage, the fertility reduction, the crop quality deterioration and the low utilization rate of the fertilizer along with the large-scale application of the chemical fertilizer, thereby causing the continuous rise of the agricultural production cost and the increasingly obvious damage to the ecological environment.
Foliar fertilizer is a type of fertilizer in which nutrient elements are applied to the surface of the leaves of crops to exert their functions by the absorption of the leaves. Research has shown that nutrients sprayed onto plant surfaces can be absorbed into the plant body through the "gaps" in the plant's leaf stomata and cuticle and thus be transformed and utilized. A plurality of tiny air holes are distributed on the surface of the plant leaf, the tiny air holes can perform the function of gas exchange and facilitate the entering of nutrient substances, and the tiny air holes are the main channels for plants to absorb the leaf fertilizer.
The foliar fertilizer is divided into two existing forms of powder and liquid, and the current popular foliar fertilizer in China is divided into two categories of nutrition and function according to the function. The nutritional foliar fertilizer mainly comprises trace elements, and the functional foliar fertilizer mainly comprises inorganic nutritional elements, plant growth regulators, biological active substances such as amino acids and humic acids, bactericides and the like. The nutritional foliar fertilizer is only used for externally supplementing nutritional elements, the functional foliar fertilizer contains various bioactive substances which have a stimulating effect on plant growth, and is doped with bactericide and pesticide components for preventing diseases and insect pests, so that the crops have the characteristic of stress resistance after long-term use, and the pesticide residues of the crops are harmful to human bodies.
A series of policies advocate ecological organic planting in recent two years in China, and the reduction of the use of chemical fertilizers and pesticides is strongly required. And the domestic diet safety of the national people is ensured from the source until the complete stop is achieved. Therefore, research and development of the foliar fertilizer which can start from the cells of crops and improve the immunity and disease resistance of the crops, so that the roots and leaves of the crops can keep good nutrition absorption and growth microenvironment, the use of pesticides is reduced and refused, the green, ecological and safe crop products are ensured, and the foliar fertilizer becomes a necessary task and responsibility.
Disclosure of Invention
In order to solve the problems in the prior art, the invention provides an ecological, organic and efficient agricultural and forestry crop foliar bacterial liquid and a preparation method thereof. The agricultural and forestry crop foliar bacterial liquid disclosed by the invention can start from a crop cell survival microenvironment, promote the crop immune cells to enhance the immunity and metabolism capability, and better absorb organic green nutrients, so that the capacities of crops in absorbing nutrition, resisting plant diseases and insect pests, rapidly growing and the like are improved. The agricultural and forestry crop foliar bacterial liquid has obvious effects on improving the disease resistance of agricultural and forestry crops, expelling insects and preventing pests, reducing the use amount of chemical fertilizers and pesticides, removing and reducing pesticide and heavy metal residues on the surfaces of fruits and vegetables, improving the quality and the yield of the fruits and vegetables, and the like.
The technical scheme adopted by the invention is as follows:
an agricultural and forestry crop foliar bacterial liquid comprises the following raw materials:
2000-2010 parts of bio-organic fertilizer leaching solution;
0.03-0.13 parts by weight of chitosan oligosaccharide;
0.4-2.5 parts by weight of hydrolyzed feather composite amino acid;
0.5 to 4.0 weight portions of composite probiotics.
Chitosan oligosaccharide, low molecular weight chitosan (chitohexaose) extracted from marine organisms, which is the only slightly alkaline amino substance found in nature, can rapidly enter the surfaces of crop cells after being sprayed on the surfaces of roots, stems and leaves of crops to exert the special effect of the chitosan oligosaccharide, and simultaneously promotes other nutrient substances to be better absorbed and exert the synergistic effect.
Chitosan oligosaccharide as a plant vaccine has many effects: (a) the environmental safety is good. The chitosan oligosaccharide has no toxicity, is easy to decompose and has no residual influence; (b) it has no drug resistance. The chitosan oligosaccharide has unique mechanism by acting on plant cells and activating the acquired anti-Specific Absorption Rate (SAR) reaction of a plant system; (c) promoting the growth of roots, stems, leaves and fruits, improving the quality, increasing the production and income. After the chitosan oligosaccharide is used, the activity index of plant cells is enhanced, the rate and the efficiency of photosynthesis of plants are enhanced, the content of soluble protein is improved, and the plants grow vigorously and are robust. Not only promotes the growth of plants, but also can improve the endurance of crops to some short-term adverse factors such as drought, low temperature, early frost and the like, and can also enhance the resistance of soil-borne diseases including nematodes. For the fruit and vegetable plants, the plant growth vigor is obviously superior to that of a reference substance by being sprayed on the page for 3-5 times in the growth period, so that the fruit picking period is advanced, the fruit setting rate is improved, the single fruit yield is high, and the quality is obviously higher than that of a reference group; (d) and (5) bacteriostasis and antibacterial property. Experiments prove that the chitosan oligosaccharide is applied to leaf surfaces of 24 crops, can be combined with receptors of plant cells, activates the conduction and expression of disease-resistant signals, releases disease-resistant factors, prevents the invasion of external diseases and insect pests on one hand, and inhibits the growth of various plant pathogenic bacteria on the other hand.
The bio-organic fertilizer leaching solution is prepared by the following method:
(S1) taking sheep manure and mushroom residues, crushing, mixing and stirring to obtain a mixture;
(S2) adding active bacterial liquid into the mixture obtained in the step (S1), stirring and mixing, composting, and decomposing and fermenting to obtain an organic fertilizer;
(S3) adding water into the organic fertilizer, soaking for 5-7 days, and filtering and leaching to obtain a leachate, namely the bio-organic fertilizer leachate.
In the step (S1), the mass ratio of the sheep manure to the mushroom dregs is 30-95: 65-4.8;
in the step (S2), the active bacterial liquid is composed of: 0.2 to 4 percent of zymophyte, 0.08 to 1 percent of peptone and 0.05 to 0.25 percent of composite probiotics;
the composite probiotics comprise the following components: 0.0175-0.0875% of bacillus amyloliquefaciens, 0.015-0.075% of lactobacillus plantarum and 0.0175-0.0875% of saccharomyces cerevisiae;
the addition amount of the active bacterial liquid accounts for 45% of the mass of the mixture.
In the step (S2), the decomposing fermentation is performed four times, specifically: composting, turning over for the first time, and performing first decomposition fermentation when the temperature in the pile reaches 75 ℃; after the primary fermentation is finished, turning over the pile for the second time, and performing secondary decomposition fermentation when the temperature in the pile reaches 75 ℃; after the secondary fermentation is finished, turning the pile for the third time, and performing the third decomposition fermentation when the temperature in the pile is reduced to 25 ℃; and after the third fermentation is finished, turning the pile for four times, keeping the temperature in the pile unchanged at 25 ℃, and performing the fourth decomposition fermentation.
The hydrolyzed feather compound amino acid is prepared by the following method:
the feather is sterilized at high temperature, and then hydrolyzed by a near-critical hydrolysis method to prepare hydrolyzed feather amino acid.
In the specific implementation, Taikun group waste chicken feathers are used as raw materials, high-temperature sterilization is carried out, and a near-critical hydrolysis method is adopted to prepare hydrolyzed feather amino acid which is rich in various amino acids. Not only solves the problem of poultry waste pollution, but also recycles and supplements the nutrient elements of fruits, vegetables and plants.
The compound probiotic liquid is prepared by the following method:
(SS1) respectively placing bacillus amyloliquefaciens, plant lactic acid bacteria and saccharomyces cerevisiae into a strain fermentation tank, adding purified water with the mass 10 times of that of the strain, adding peptone, keeping the water temperature at 25-30 ℃, stirring once every other period of time, stirring for multiple times, and standing to obtain a bacterial liquid;
(SS2) adding purified water with the mass being 10 times of that of the bacterium liquid, keeping the water temperature at 25-30 ℃, stirring once every certain time, stirring for multiple times, and standing to obtain the compound probiotic bacterium liquid.
In the step (SS1), the mass ratio of the bacillus amyloliquefaciens, the lactobacillus plantarum and the saccharomyces cerevisiae is 15-45: 60-40: 25-15 parts of;
stirring every 2 hours for 5 times, and standing for 72 hours.
In specific implementation, the composite probiotic liquid is prepared from bacillus amyloliquefaciens, saccharomyces cerevisiae and lactobacillus plantarum prepared by Chinese academy of agricultural sciences, is prepared according to a specific proportion aiming at fruits and vegetables in Xinjiang, and has an obvious effect on the fruits and vegetables such as vegetables, grapes, apples and flat peaches under the synergistic effect of chitosan oligosaccharide.
The preparation method of the agricultural and forestry crop foliar bacterial liquid comprises the following steps:
(1) respectively taking a bio-organic fertilizer leaching solution, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacteria liquid according to the weight, and placing the bio-organic fertilizer leaching solution, the chitosan oligosaccharide, the hydrolyzed feather compound amino acid and the compound probiotic bacteria liquid in a fermentation tank;
(2) adding brown sugar into the fermentation tank, keeping a certain temperature, stirring once every a period of time, standing for a certain time, and filtering to obtain the foliage bacterial liquid of the agricultural and forestry crops.
In the step (2), the adding amount of the brown sugar accounts for 0.25 percent of the total mass of the materials in the fermentation tank.
In the step (2), the temperature is kept at 25 ℃, and the mixture is stirred for 10 minutes every 2 hours at night and is stirred for 5 times totally; stirring for 10 minutes every 4 hours in the daytime, and repeatedly stirring for 3 times;
the standing time was 48 hours.
The invention has the following beneficial effects:
(1) the raw materials of the agricultural and forestry crop foliar bacterial liquid comprise biological organic fertilizer leachate, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacterial liquid, the biological organic fertilizer leachate is used as a morphological matrix of the agricultural and forestry crop foliar bacterial liquid, the chitosan oligosaccharide is used as a core substance of a plant vaccine, the hydrolyzed feather compound amino acid and the compound probiotic bacterial liquid are added for mixing and compatibility, and finally the prepared agricultural and forestry crop foliar bacterial liquid is subjected to the action of the chitosan oligosaccharide, so that the effects of the biological organic fertilizer leachate, the hydrolyzed feather compound amino acid and the compound probiotic bacterial liquid are synergistically exerted, and the synergistic effects of supplementing each other, enhancing immunity and solving the source are achieved in the aspects of promoting plant growth, inhibiting harmful bacteria, improving the yield of single-column fruit thinning, improving the quality of fruits and vegetables, reducing pesticide and heavy metal residues and the like. The agricultural and forestry crop foliar bacterial liquid provides possibility for ecological green planting, greatly reduces the use amount of pesticides and chemical fertilizers, and promotes the development of agricultural planting to the aspects of ecological cycle and green safety.
(2) The agricultural and forestry crop foliar bacterial liquid takes the sheep manure and the mushroom residues as main substances of a bio-organic fertilizer leaching solution, fully utilizes the optimal organic nutrient components in the sheep manure and the fungal polysaccharide in the mushroom residues, and has multiple nutritional effects on plants. The method comprises the steps of mixing sheep manure and mushroom residues with zymophyte, then carrying out decomposition fermentation to obtain an organic fertilizer without pathogenic bacteria, carrying out a water extraction leaching process to obtain an organic fertilizer leaching solution serving as a foliar fertilizer liquid carrier, wherein green ecological nutrient elements of the organic fertilizer leaching solution, hydrolyzed feather compound amino acid and specially selected compound probiotic bacteria liquid play a role of supplementing each other to plant cells under the action of chitosan oligosaccharide.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the embodiments described are merely exemplary of the invention, and not of the invention in its entirety. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without any inventive step, are within the scope of the present invention.
In the following examples, 1 part by weight represents 1 kg.
Example 1
The embodiment provides an agriculture and forestry crop foliage bacteria liquid, and the raw materials include:
2000kg of bio-organic fertilizer leaching solution;
0.1kg of chitosan oligosaccharide;
0.8kg of hydrolyzed feather compound amino acid;
2.0kg of compound probiotic bacteria liquid.
The bio-organic fertilizer leaching solution is prepared by the following method:
(S1) taking sheep manure and mushroom residues according to the mass ratio of 80:19.5, crushing, mixing and stirring to obtain a mixture;
(S2) adding active bacterial liquid into the mixture obtained in the step (S1); the active bacterial liquid comprises the following components: 0.2% of zymophyte, 0.08% of peptone, 0.05% of composite probiotics and the balance of water; the composite probiotics comprise the following components: 0.0175% of bacillus amyloliquefaciens, 0.015% of plant lactic acid bacteria and 0.0175% of saccharomyces cerevisiae;
stirring, mixing, composting, and decomposing and fermenting to obtain an organic fertilizer; the decomposing fermentation is repeated for four times, and the specific operation is as follows: composting, turning over for the first time, and performing first decomposition fermentation for 3 days when the temperature in the pile reaches 75 ℃; after the first fermentation is finished, turning the pile for the second time, and performing second decomposition fermentation for 3 days when the temperature in the pile reaches 75 ℃; after the second fermentation is finished, turning the pile for the third time, and performing third-time mature fermentation for 3 days when the temperature in the pile is reduced to 25 ℃; after the third fermentation is finished, turning the pile for the fourth time, keeping the temperature in the pile unchanged at 25 ℃, performing the fourth decomposition fermentation for 3 days, and obtaining the organic fertilizer after the fermentation is finished;
(S3) adding 2 tons of water into 80kg of the organic fertilizer, soaking for 5 days, and stirring for 10 minutes for 2 times per day. And filtering and leaching after 5 days to obtain a leaching solution, namely the bio-organic fertilizer leaching solution.
The hydrolyzed feather compound amino acid is prepared by the following method: the method is characterized in that Taikun group waste chicken feathers are used as raw materials, the chicken feathers are sterilized at high temperature, and then hydrolyzed by a near-critical hydrolysis method to prepare hydrolyzed feather amino acid.
The compound probiotic liquid is prepared by the following method:
(SS1) according to the mass ratio of 35: 50: 20 respectively placing bacillus amyloliquefaciens, plant lactic acid bacteria and saccharomyces cerevisiae in a strain fermentation tank, adding purified water with the mass 10 times of that of the strain, adding peptone, keeping the water temperature at 25 ℃, stirring once every 2 hours for 5 times, and standing for 72 hours to obtain a bacterial liquid;
(SS2) adding purified water with the mass being 10 times that of the bacterium liquid, keeping the water temperature at 25 ℃, stirring once every other period of time, and standing after stirring for multiple times to obtain the compound probiotic bacterium liquid.
The preparation method of the agricultural and forestry crop foliar bacterial liquid further comprises the following steps:
(1) respectively taking a bio-organic fertilizer leaching solution, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacteria liquid according to the weight, and placing the bio-organic fertilizer leaching solution, the chitosan oligosaccharide, the hydrolyzed feather compound amino acid and the compound probiotic bacteria liquid in a fermentation tank;
(2) adding brown sugar accounting for 0.25 percent of the total mass of the materials into the fermentation tank, keeping the temperature at 25 ℃, stirring for 10 minutes every 2 hours at night, and stirring for 5 times totally; stirring for 10 minutes every 4 hours in the daytime, and repeatedly stirring for 3 times; standing for 48 hours, and filtering to obtain the agricultural and forestry crop foliar bacterial liquid.
Example 2
The embodiment provides an agriculture and forestry crop foliage bacteria liquid, and the raw materials include:
2010kg of bio-organic fertilizer leaching solution;
0.03kg of chitosan oligosaccharide;
0.4kg of hydrolyzed feather compound amino acid;
0.5kg of composite probiotic bacteria liquid.
The bio-organic fertilizer leaching solution is prepared by the following method:
(S1) taking sheep manure and mushroom residues according to the mass ratio of 30:4.8, crushing, mixing and stirring to obtain a mixture;
(S2) adding active bacterial liquid into the mixture obtained in the step (S1); the active bacterial liquid comprises the following components: 4% of zymophyte, 1% of peptone, 0.25% of composite probiotics and the balance of water; the composite probiotics comprise the following components: 0.0875% of bacillus amyloliquefaciens, 0.075% of lactobacillus plantarum and 0.0875% of saccharomyces cerevisiae;
stirring, mixing, composting, and decomposing and fermenting to obtain an organic fertilizer; the decomposing fermentation was repeated four times. The specific operation is as follows: composting, turning over for the first time, and performing first decomposition fermentation for 5 days when the temperature in the pile reaches 75 ℃; after the first fermentation is finished, turning the pile for the second time, and performing second decomposition fermentation for 5 days when the temperature in the pile reaches 75 ℃; after the second fermentation is finished, turning the pile for the third time, and performing third-time mature fermentation for 5 days when the temperature in the pile is reduced to 25 ℃; after the third fermentation is finished, turning the pile for the fourth time, keeping the temperature in the pile unchanged at 25 ℃, performing the fourth decomposition fermentation for 5 days, and obtaining the organic fertilizer after the fermentation is finished;
(S3) adding 2 tons of water into 80kg of the organic fertilizer, soaking for 7 days, and stirring for 10 minutes for 3 times per day. Filtering and leaching after 7 days to obtain a leaching solution, namely the bio-organic fertilizer leaching solution.
The hydrolyzed feather compound amino acid is prepared by the following method: the method is characterized in that Taikun group waste chicken feathers are used as raw materials, the chicken feathers are sterilized at high temperature, and then hydrolyzed by a near-critical hydrolysis method to prepare hydrolyzed feather amino acid.
The compound probiotic liquid is prepared by the following method:
(SS1) according to the mass ratio of 15: 40: 15 respectively taking bacillus amyloliquefaciens, plant lactic acid bacteria and saccharomyces cerevisiae, placing the bacillus amyloliquefaciens, the plant lactic acid bacteria and the saccharomyces cerevisiae in a strain fermentation tank, adding purified water with the mass of 10 times of that of the strain, adding peptone, keeping the water temperature at 30 ℃, stirring once every 2 hours for 5 times, and standing for 72 hours to obtain a bacterial liquid;
(SS2) adding purified water with the mass being 10 times that of the bacterial liquid, keeping the water temperature at 30 ℃, stirring once every certain time, and standing after stirring for multiple times to obtain the compound probiotic bacterial liquid.
The preparation method of the agricultural and forestry crop foliar bacterial liquid further comprises the following steps:
(1) respectively taking a bio-organic fertilizer leaching solution, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacteria liquid according to the weight, and placing the bio-organic fertilizer leaching solution, the chitosan oligosaccharide, the hydrolyzed feather compound amino acid and the compound probiotic bacteria liquid in a fermentation tank;
(2) adding brown sugar accounting for 0.25 percent of the total mass of the materials into the fermentation tank, keeping the temperature at 25 ℃, stirring for 10 minutes every 2 hours at night, and stirring for 5 times totally; stirring for 10 minutes every 4 hours in the daytime, and repeatedly stirring for 3 times; standing for 48 hours, and filtering to obtain the agricultural and forestry crop foliar bacterial liquid.
Example 3
The embodiment provides an agriculture and forestry crop foliage bacteria liquid, and the raw materials include:
2010kg of bio-organic fertilizer leaching solution;
0.13kg of chitosan oligosaccharide;
0.5kg of hydrolyzed feather compound amino acid;
4.0kg of composite probiotic bacteria liquid.
The bio-organic fertilizer leaching solution is prepared by the following method:
(S1) taking sheep manure and mushroom residues according to the mass ratio of 30:4.8, crushing, mixing and stirring to obtain a mixture;
(S2) adding active bacterial liquid into the mixture obtained in the step (S1); the active bacterial liquid comprises the following components: 1.2% of zymophyte, 0.5% of peptone, 0.15% of composite probiotics and the balance of water; the composite probiotics comprise the following components: 0.05% of bacillus amyloliquefaciens, 0.045% of lactobacillus plantarum and 0.05% of saccharomyces cerevisiae;
stirring, mixing, composting, and decomposing and fermenting to obtain an organic fertilizer; the decomposing fermentation was repeated four times. The specific operation is as follows: composting, turning over for the first time, and performing first decomposition fermentation for 5 days when the temperature in the pile reaches 75 ℃; after the first fermentation is finished, turning the pile for the second time, and performing second decomposition fermentation for 5 days when the temperature in the pile reaches 75 ℃; after the second fermentation is finished, turning the pile for the third time, and performing third-time mature fermentation for 5 days when the temperature in the pile is reduced to 25 ℃; after the third fermentation is finished, turning the pile for the fourth time, keeping the temperature in the pile unchanged at 25 ℃, performing the fourth decomposition fermentation for 5 days, and obtaining the organic fertilizer after the fermentation is finished;
(S3) adding 2 tons of water into 80kg of the organic fertilizer, soaking for 7 days, and stirring for 10 minutes for 3 times per day. Filtering and leaching after 7 days to obtain a leaching solution, namely the bio-organic fertilizer leaching solution.
The hydrolyzed feather compound amino acid is prepared by the following method: the method is characterized in that Taikun group waste chicken feathers are used as raw materials, the chicken feathers are sterilized at high temperature, and then hydrolyzed by a near-critical hydrolysis method to prepare hydrolyzed feather amino acid.
The compound probiotic liquid is prepared by the following method:
(SS1) according to a mass ratio of 45: 60: 25 respectively placing bacillus amyloliquefaciens, plant lactic acid bacteria and saccharomyces cerevisiae in a strain fermentation tank, adding purified water with the mass 10 times of that of the strain, adding peptone, keeping the water temperature at 30 ℃, stirring once every 2 hours for 5 times, and standing for 72 hours to obtain a bacterial liquid;
(SS2) adding purified water with the mass being 10 times that of the bacterial liquid, keeping the water temperature at 30 ℃, stirring once every certain time, and standing after stirring for multiple times to obtain the compound probiotic bacterial liquid.
The preparation method of the agricultural and forestry crop foliar bacterial liquid further comprises the following steps:
(1) respectively taking a bio-organic fertilizer leaching solution, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacteria liquid according to the weight, and placing the bio-organic fertilizer leaching solution, the chitosan oligosaccharide, the hydrolyzed feather compound amino acid and the compound probiotic bacteria liquid in a fermentation tank;
(2) adding brown sugar accounting for 0.25 percent of the total mass of the materials into the fermentation tank, keeping the temperature at 25 ℃, stirring for 10 minutes every 2 hours at night, and stirring for 5 times totally; stirring for 10 minutes every 4 hours in the daytime, and repeatedly stirring for 3 times; standing for 48 hours, and filtering to obtain the agricultural and forestry crop foliar bacterial liquid.
The agricultural and forestry crop foliar bacterial liquid is used in actual agricultural production, the area of the fruits and vegetables which are benefited per year reaches 33.33 ten thousand mu, the income of farmers is increased by 29997 ten thousand yuan (taking grapes as an example, the new yield per mu is 180kg, and the purchase price per mu is 5.00 yuan/kg), the heavy metal and pesticide residues of the fruits and vegetables are reduced by 53%, and the usage amount of chemical fertilizers is reduced by 35%.
The above description is only for the specific embodiments of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. The agricultural and forestry crop foliar bacterial liquid is characterized by comprising the following raw materials:
2000-2010 parts of bio-organic fertilizer leaching solution;
0.03-0.13 parts by weight of chitosan oligosaccharide;
0.4-2.5 parts by weight of hydrolyzed feather composite amino acid;
0.5-4.0 parts of composite probiotic bacteria liquid.
2. The agricultural and forestry crop foliar bacterial liquid according to claim 1, wherein the bio-organic fertilizer leaching solution is prepared by a method comprising the following steps:
(S1) taking sheep manure and mushroom residues, crushing, mixing and stirring to obtain a mixture;
(S2) adding active bacterial liquid into the mixture obtained in the step (S1), stirring and mixing, composting, and decomposing and fermenting to obtain an organic fertilizer;
(S3) adding water into the organic fertilizer, soaking for 5-7 days, and filtering and leaching to obtain a leachate, namely the bio-organic fertilizer leachate.
3. The agricultural and forestry crop foliar bacterial liquid according to claim 1, wherein in the step (S1), the mass ratio of the sheep manure to the mushroom slag is 30-95: 4.8-65;
in the step (S2), the active bacterial liquid is composed of: 0.2-4% of zymophyte, 0.08-1% of peptone, 0.05-0.25% of composite probiotics and the balance of water;
the composite probiotics comprise the following components: 0.0175-0.0875% of bacillus amyloliquefaciens, 0.015-0.075% of lactobacillus plantarum and 0.0175-0.0875% of saccharomyces cerevisiae;
the addition amount of the active bacterial liquid accounts for 45% of the mass of the mixture.
4. The agricultural and forestry crop foliar bacterial liquid according to claim 1, wherein in the step (S2), the decomposing fermentation is performed four times, specifically including: composting, turning over for the first time, and performing first decomposition fermentation when the temperature in the pile reaches 75 ℃; after the primary fermentation is finished, turning over the pile for the second time, and performing secondary decomposition fermentation when the temperature in the pile reaches 75 ℃; after the secondary fermentation is finished, turning the pile for the third time, and performing the third decomposition fermentation when the temperature in the pile is reduced to 25 ℃; and after the third fermentation is finished, turning the pile for four times, keeping the temperature in the pile unchanged at 25 ℃, and performing the fourth decomposition fermentation.
5. The agricultural and forestry crop foliar bacterial liquid of claim 1, wherein the hydrolyzed feather compound amino acid is prepared by the following method:
the feather is sterilized at high temperature, and then hydrolyzed by a near-critical hydrolysis method to prepare hydrolyzed feather amino acid.
6. The agricultural and forestry crop foliar bacterial liquid of claim 1, wherein the compound probiotic bacterial liquid is prepared by the following method:
(SS1) respectively placing bacillus amyloliquefaciens, plant lactic acid bacteria and saccharomyces cerevisiae into a strain fermentation tank, adding purified water with the mass 10 times of that of the strain, adding peptone, keeping the water temperature at 25-30 ℃, stirring once every other period of time, stirring for multiple times, and standing to obtain a bacterial liquid;
(SS2) adding purified water with the mass being 10 times that of the bacterial liquid, adding peptone, keeping the water temperature at 25-30 ℃, stirring once every other period of time, and standing after stirring for multiple times to obtain the compound probiotic bacterial liquid.
7. The agricultural and forestry crop foliar bacterial liquid according to claim 1, wherein in the step (SS1), the mass ratio of bacillus amyloliquefaciens, lactobacillus plantarum and saccharomyces cerevisiae is 15-45: 40-60: 15-25;
stirring every 2 hours for 5 times, and standing for 72 hours.
8. The method for preparing the agricultural and forestry crop foliar bacterial liquid, as set forth in claims 1 to 7, is characterized by comprising the following steps:
(1) respectively taking a bio-organic fertilizer leaching solution, chitosan oligosaccharide, hydrolyzed feather compound amino acid and compound probiotic bacteria liquid according to the weight, and placing the bio-organic fertilizer leaching solution, the chitosan oligosaccharide, the hydrolyzed feather compound amino acid and the compound probiotic bacteria liquid in a fermentation tank;
(2) adding brown sugar into the fermentation tank, keeping a certain temperature, stirring once every a period of time, standing for a certain time, and filtering to obtain the foliage bacterial liquid of the agricultural and forestry crops.
9. The method for preparing the foliar bacterial liquid of agricultural and forestry crops as claimed in claim 8, wherein in the step (2), the addition amount of brown sugar is 0.25% of the total mass of the materials in the fermentation tank.
10. The method for preparing the foliar bacterial liquid of agricultural and forestry crops as claimed in claim 8, wherein in the step (2), the temperature is kept at 25 ℃, and the mixture is stirred for 10 minutes at intervals of 2 hours at night for 5 times; stirring for 10 minutes every 4 hours in the daytime, and repeatedly stirring for 3 times;
the standing time was 48 hours.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110794471.4A CN113354474A (en) | 2021-07-14 | 2021-07-14 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202110794471.4A CN113354474A (en) | 2021-07-14 | 2021-07-14 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN113354474A true CN113354474A (en) | 2021-09-07 |
Family
ID=77539380
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202110794471.4A Pending CN113354474A (en) | 2021-07-14 | 2021-07-14 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN113354474A (en) |
Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106495763A (en) * | 2016-11-01 | 2017-03-15 | 西北农林科技大学 | A kind of preparation method of compost extraction |
| CN108623388A (en) * | 2018-06-27 | 2018-10-09 | 齐河百丰生物技术有限公司 | A kind of disease-resistant foliage fertilizer and preparation method thereof |
| CN108821844A (en) * | 2018-05-25 | 2018-11-16 | 来安县杭姑娘生态农业有限公司 | A kind of organic vegetable Liquid Fertilizer |
| CN109956801A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development |
| CN109956782A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer promoting root growth |
| CN111253181A (en) * | 2019-04-01 | 2020-06-09 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Drought-resistant foliar fertilizer |
| CN111320507A (en) * | 2020-04-14 | 2020-06-23 | 安徽卓砺环保科技有限公司 | Functional liquid fertilizer, preparation method thereof and cotton fertilization method |
-
2021
- 2021-07-14 CN CN202110794471.4A patent/CN113354474A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106495763A (en) * | 2016-11-01 | 2017-03-15 | 西北农林科技大学 | A kind of preparation method of compost extraction |
| CN109956801A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer and preparation method thereof for fruit corn flower bud development |
| CN109956782A (en) * | 2017-12-25 | 2019-07-02 | 郴州市通源生物科技有限公司 | A kind of liquid fertilizer promoting root growth |
| CN108821844A (en) * | 2018-05-25 | 2018-11-16 | 来安县杭姑娘生态农业有限公司 | A kind of organic vegetable Liquid Fertilizer |
| CN108623388A (en) * | 2018-06-27 | 2018-10-09 | 齐河百丰生物技术有限公司 | A kind of disease-resistant foliage fertilizer and preparation method thereof |
| CN111253181A (en) * | 2019-04-01 | 2020-06-09 | 广东省生物工程研究所（广州甘蔗糖业研究所） | Drought-resistant foliar fertilizer |
| CN111320507A (en) * | 2020-04-14 | 2020-06-23 | 安徽卓砺环保科技有限公司 | Functional liquid fertilizer, preparation method thereof and cotton fertilization method |
Non-Patent Citations (1)
| Title |
|---|
| 张择瑞: "《环境生物化学基础 第2版》", 31 July 2017, 合肥工业大学出版社 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103113167B (en) | Compound microbial organic fertilizer and preparation method thereof | |
| US8530220B2 (en) | Microorganisms, microbial phosphate fertilizers and methods for preparing such microbial phosphate fertilizers | |
| CN103044138B (en) | Preparation method for composite biological bacterial fertilizer | |
| CN105130589A (en) | Multifunctional bioorganic liquid fertilizer and production method thereof | |
| CN104446708A (en) | Microbial fertilizer as well as preparation method and purpose thereof | |
| CN103193543A (en) | Pollution-free biological organic bacteria fertilizer with nutrient balancing function | |
| CN102295494B (en) | Melon-bean fermentation broth, fermentation fertilizer and its preparation method | |
| CN104478501A (en) | Preparation method of composite biological organic fertilizer | |
| CN109232085A (en) | Yellow peach bio-organic fertilizer special and its preparation method and application | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN107721713A (en) | A kind of microbial compound bacterial fertilizer and preparation method and application | |
| CN111348957A (en) | Enzyme biological water flush fertilizer with comprehensive functions and preparation process | |
| CN102584361A (en) | Manufacturing method of multienzyme functional bacterial fertilizer | |
| CN102219613B (en) | Green multifunctional compound ecological organic liquid fertilizer and processing method thereof | |
| CN103739391B (en) | A kind of leaf vegetables special composite biological is fertile | |
| CN103708970B (en) | Special compound biological fertilizer for tobacco | |
| CN104692969A (en) | Biological humic acid liquid fertilizer and preparation method thereof | |
| CN102503709A (en) | Multienzyme microbial fertilizer | |
| CN102503695A (en) | Microorganism bacterial fertilizer and preparation method thereof | |
| CN107082657A (en) | A kind of organic fertilizer and preparation method thereof | |
| CN106748213A (en) | A kind of amino acid Water soluble fertilizer and preparation method thereof | |
| CN102550246A (en) | Application method of photosynthetic bacteria culture solution in watermelon protected cultivation | |
| CN110981638A (en) | Biological organic fertilizer with saline-alkali soil improvement effect and preparation process thereof | |
| CN101823909B (en) | Traditional chinese medicine microbial fertilizer and preparation method thereof | |
| CN109503228A (en) | A kind of liquid bacterial manure and preparation method thereof using Ludwig enterobacteria fermented garlic stalk |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20210907 |