CN109956782A - A kind of liquid fertilizer promoting root growth - Google Patents
A kind of liquid fertilizer promoting root growth Download PDFInfo
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- CN109956782A CN109956782A CN201711427736.7A CN201711427736A CN109956782A CN 109956782 A CN109956782 A CN 109956782A CN 201711427736 A CN201711427736 A CN 201711427736A CN 109956782 A CN109956782 A CN 109956782A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
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Abstract
The invention discloses a kind of liquid fertilizer and preparation method thereof for promoting root growth, which includes 1000 parts of active liquid fertilizer substrate preparation, multiple 0.1 ~ 2 part of phthalein nucleic acid, 0.1 ~ 1.5 part of chitosan oligosaccharide, 20 ~ 32 parts of calcium ammonium nitrate, amino acid 1 ~ 12 part, 17 ~ 27 parts of potassium dihydrogen phosphate, 9 ~ 20 parts of magnesium sulfate, 1 ~ 10 part of microelement；The liquid fertilizer has not only effectively facilitated the growth and development of capsicum rhizome, while having significant facilitation to root amount, Root Distribution and root class group Chengdu.
Description
Technical field
The invention belongs to the technical fields of liquid fertilizer, more particularly, to a kind of liquid fertilizer for promoting root growth
And preparation method thereof.
Background technique
Capsicum is 1 year or limited herbaceos perennial, and fruit is usually in cone or oblong, in green when prematurity
Color becomes cerise, green or purple after mature, with red most commonly seen.The fruit of capsicum has because pericarp contains capsaicine
Pungent can improve a poor appetite.Ascorbic content occupies first in vegetables in capsicum, originate in Mexico, at the end of the Ming Dynasty be passed in
State.Capsicum originates in middle Latin America torrid areas, and the country of origin is Mexico.Sichuan, Guizhou, lake are mainly distributed in China
South, Yunnan, Shaanxi, Henan (Xichuan County), Hebei province Jize County and Inner Mongol Tuoketuo County.
Streptomyces (streptomyces) is a category for Actinomycetal.Substrate mycelium is not broken, and aerial hyphae usually sends out
Educate good, the fibrillae of spores of formation long (sometimes short).Spore cannot move, and often have the shapes jewelry such as wart, thorn or hair on epitheca.Strepto-
Bacterium is widely present in soil, is Filamentous gram-positive bacterium, mainly by sporogenesis, most streptomycetes are to people
Body is harmless.Streptomycete is the main source for generating various antibiotic, the antibiotic derived from streptomycete include erythromycin, tetracycline,
Streptomysin, chloramphenicol, neomycin, nystatin, kanamycins, cycloheximide and anphotericin etc., therefore, for streptomycete
Existing research focuses primarily upon bacterial strain and generates ability and size of antibacterial substance etc..
The root system of beauty green pepper is not only the absorption organ of nutrient and water, can also be experienced produced by the nutrient situation of soil
Chemical signal, and these signals are transmitted to overground part, to control the physiological activity of overground part organ, and root system is strong
Health, which directly affects the development of plant later period, leads to capsicum root lesion since nutrient is not complete in existing agricultural fertilizer, causes to produce fruit
It is small, it the yield of capsicum has been seriously affected, therefore, establishes and keeps a healthy root system, for beauty green pepper stem, leaf, fruit
Normal development is very important, even more important especially for the seedling just planted.
Summary of the invention
It is raw to provide a kind of promotion rhizome for the problem of the technical problem to be solved by the present invention is to overcome existing capsicum root growths
Long liquid fertilizer and preparation method thereof.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of liquid fertilizer promoting root growth, by the material composition of following parts by weight:
1000 parts of active liquid fertilizer substrate preparation, multiple 0.1~2 part of phthalein nucleic acid, 0.1~1.5 part of chitosan oligosaccharide, calcium ammonium nitrate 20
~32 parts, amino acid 1~12 part, 17~27 parts of potassium dihydrogen phosphate, 9~20 parts of magnesium sulfate, 1~10 part of microelement；The liquid
Total nitrogen content >=2.8mg/g in body fertilizer, amino nitrogen content >=0.4mg/g, the content of organic matter >=120g/L.
The present invention acts on changeable soil flora by chitosan oligosaccharide, promotes beneficial microorganism growth, induces the anti-of plant
Characteristic of disease generates immune and killing effect to a variety of fungies, bacterium and virus.Multiple phthalein nucleic acid in the present invention can promote plant elongation
Growth promotes the growth of rhizome, bud, so that seedling and growth plant fast-growth is formed strong sprout and strengthens strain, increases the weight of base of leaf strain,
Extend leaf senile simultaneously, promotes the reduction speed of chlorophyll, protein and plasm content.
Further, it is preferable to which the liquid fertilizer is by following material composition: 1000 parts of active liquid fertilizer substrate preparation, multiple phthalein
0.5~1 part of nucleic acid, 0.3~0.9 part of chitosan oligosaccharide, 23~30 parts of calcium ammonium nitrate, 4~10 parts of amino acid, potassium dihydrogen phosphate 19~25
Part, 12~17 parts of magnesium sulfate, 4~8 parts of microelement；Total nitrogen content >=2.8mg/g in the liquid fertilizer, amino nitrogen content >=
0.4mg/g, the content of organic matter >=120g/L.
Further, it is preferable to 1000 parts of active liquid fertilizer substrate preparation, multiple 0.7 part of phthalein nucleic acid, 0.6 part of chitosan oligosaccharide, nitric acid
27 parts of ammonium calcium, 7 parts of amino acid, 22 parts of potassium dihydrogen phosphate, 15 parts of magnesium sulfate, 6 parts of microelement；Total nitrogen contains in the liquid fertilizer
Amount >=2.8mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter >=120g/L.
Further, the active liquid fertilizer substrate preparation is obtained by streptomyces bacterial strain " TY-001# " fermentation；
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, into
Row mashing, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~
7.6；
Further, the streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's microbial bacteria on March 24th, 2017
Kind collection, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is China
XianLie Middle Road, GuangZhou City, GuangDong Province 100 five building, Institute of Micro-biology, province laboratory building.
Further, the ratio of N, P, K are 1.5~3:1:1~2, preferably 8:5:7 in the liquid fertilizer.
Further, the ratio between calcium, magnesium, phosphorus are 1~2:1:1~2, preferably 5:3:5 in the liquid fertilizer.
Further, the microelement contains boron, zinc, copper, molybdenum, iron.
A kind of preparation method of the liquid fertilizer promoting root growth, comprising the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then past
After sugar is added in filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4~7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, continuing fermentation 7~8 days at 20~28 DEG C
Afterwards, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. multiple phthalein nucleic acid, chitosan oligosaccharide, ammonium nitrate the configuration of liquid fertilizer: are added toward the active liquid fertilizer substrate preparation
Calcium, amino acid, potassium dihydrogen phosphate, magnesium sulfate, microelement obtain the liquid fertilizer.
The streptomyces bacterial strain " TY-001# " was preserved in Guangdong Province's Microbiological Culture Collection on March 24th, 2017
The heart, classification naming Streptomyces., deposit number are GDMCC No.:60152, and preservation address is that Guangdong province, China is wide
State city martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
Further, the pre-fermented step are as follows:
S11. into dregs of beans plus water, and stir it is full and uniform, until dregs of beans is reached with clenching of the hand drips into the state of line i.e.
It can；
S12. it is covered on dregs of beans surface with film, left no gaps, timing starts pre fermentation process；
S13. the pre fermentation time is 3~5 days；After starting fermentation 2 days, thermometer is inserted into dregs of beans depths, in fermentation raw material
When portion temperature >=40 DEG C, pre fermentation dregs of beans is inside and outside overturn once, continue fermentation 2~3 days.
Preferably, adding water to be settled to concentration sugared in culture medium in the step S1 is 100g/L.
Preferably, its amino-acid nitrogen content >=4.8g/L after the active liquid fertilizer substrate preparation is stored 15 days.
Preferably, in the liquid spawn every milliliter have 200,000,000 viable bacterias；The ratio of the liquid spawn and culture medium is
165g:250L。
Preferably, the morphological feature of streptomyces bacterial strain " TY-001# " are as follows: bacterium colony is rounded, milky, the smooth of the edge,
Show there is condensed milk shape gloss；Spore Gram's staining is positive.
A kind of cultural method of the streptomyces bacterial strain (Streptomyces), comprising the following steps:
Y1. streptomyces bacterial strain " TY-001# " is taken to line on solid medium, 25~30 DEG C of inversion activation cultures 3~7
It, is made bacterial strain after activation；
Y2. strain inoculated is into fluid nutrient medium after taking step Y1 activation obtained, temperature is 25~30 DEG C, revolution is
Under conditions of 150~220 revs/min, shaking table culture 3~7 days, bacterium solution is obtained.
Preferably, every liter of component of the fluid nutrient medium is as follows: soybean, dregs of beans 100g being taken to wear into soya-bean milk filtering, waste；It is white
Granulated sugar, sucrose or brown sugar 100g；Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
Water is settled to 1 liter, and pH is 7.4~7.6.
Preferably, every liter of component of the solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g,
15~25g of agar, water are settled to 1 liter, and pH is 7.4~7.6.
Compared with prior art, the invention has the following advantages:
Chitosan oligosaccharide and multiple phthalein nucleic acid are mixed in a certain ratio and efficiently promote capsicum root growth, enhance plant resistance, group
Point green natural, nuisanceless, each fertilizer components scientific matching, can effectively facilitate plant quickly, healthy growth, while being avoided that again
Extra nutrient loss substantially increases the utilization rate of chemical fertilizer, effectively prevent environmental pollution.
Various microelements needed for liquid fertilizer of the invention is rich in capsicum early growth, to crop because of environment nutritional deficiency gas
It the runt seedling caused by physiological diseases such as waits, has remedied good recovery effect after weak seedling and calamity, while quality and capsicum for fruit
It absorbs nutrition and has established good basis.
Liquid fertilizer of the invention increases 35% compared to comparative example to total root item number of beauty green pepper, root amount, Root Distribution
It increases significantly with root class group Chengdu relative comparison group.Compared with the control group, beauty green pepper pole length increases 29.6%, crown diameter
36.5% is increased, body of rod leaf color is dark green, and chlorophyll content increases obvious.However, the liquid fertilizer is raw to the plant of small tomato
Long only more small effect.As it can be seen that liquid fertilizer of the invention has very significant effect to the strong plantlets and rootage of capsicum, it is right
The vegetables and fruits of different cultivars selectivity with higher.
Streptomyces bacterial strain " TY-001# " provided by the invention, the bacterial strain can adapt to aerobic fementation under hyperosmosis and train
It supports.
Specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, but embodiment the present invention is not done it is any type of
It limits.Unless stated otherwise, the present invention uses reagent, method and apparatus is the art conventional reagents, method and apparatus.
Unless stated otherwise, following embodiment agents useful for same and material are commercially available.
The separation and identification of 1 streptomyces bacterial strain of embodiment (Streptomyces)
1. experimental material and condition
(1) in the collected pedotheque in Hunan Province Chenzhou City somewhere.
(2) every liter of component of fluid nutrient medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, water are settled to 1
It rises, pH 7.5.
Every liter of component of solid medium is as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, 15~25g of agar,
Water is settled to 1 liter, pH 7.5.
Culture medium packing, high-pressure sterilizing pot sterilize (121 DEG C, 30min).
(3) aseptic technique: all vessel and apparatus must sterilize (121 DEG C, 30min) through high-pressure sterilizing pot, inoculation
Equal operations carry out in superclean bench.
(4) strain culturing condition: being placed in culture in 25 ± 1 DEG C of illumination insulating boxs (14L:10D), after bacterium colony is formed, turns
The inclined-plane PDA is moved on to, then is transferred in 5 DEG C of refrigerators and saves.
2. strain isolation and screening
(1) separation obtains a streptomycete category bacterial strain T0 from the pedotheque in Hunan Province Chenzhou City somewhere.
(2) by inclined-plane squamous subculture in isolated streptomyces bacterial strain T0 access solid medium, then 5 DEG C of ice are transferred to
It is saved in case.
(3) by the bacterial strain T0 of squamous subculture in fluid nutrient medium, on oscillation shaking table under the conditions of 220 revs/min, 25 DEG C
Culture 5 days.
(4) the T0 liquid bacteria after cultivating 5 days, is placed at 20 watts of ultraviolet radiator 25cm and irradiates 2 hours.
(5) it will be cultivated 5 days in the T0 liquid bacteria access solid slope culture medium after irradiation 25 DEG C, then choose and grow most
Good surviving colonies access solid medium squamous subculture.
(6) several processes all over step (2)~(5) are repeated to recycle.Each in repetitive process circulation, solid medium
All increase by 1 gram with the sodium chloride dosage in fluid nutrient medium, until the sodium chloride dosage in solid medium and fluid nutrient medium
Reach 25 grams.The healthy and strong bacterial strain finally survived is subjected to squamous subculture, then is transferred in 5 DEG C of refrigerators and saves.
(7) the osmophilic strain bacterial strain T0 that will be filtered out above carries out fermentation production run.Through 7 days 25 DEG C open wide it is aerobic
After fermentation, amino nitrogen in fermentation liquid (measuring ammonia nitrogen standard detection by formol titration) is measured, amino nitrogen is picked out and contains
(>=0.7mg/L) highest bacterial strain is measured as final production bacterial strain, is named " TY-001# ".
Culture medium used in fermentation production run is as follows: taking soybean 100g to wear into soya-bean milk filtering, abandons bean dregs；White granulated sugar
100g is settled to 1000mL, pH 7.5.
3. the identification of bacterial strain " TY-001# "
(1) Morphological Identification
Morphological feature are as follows: bacterium colony is rounded, milky, the smooth of the edge, shows there is condensed milk shape gloss；Spore Gram's staining
It is positive.
Morphological Identification shows that the bacterial strain belongs to streptomyces.
In conclusion bacterial strain " TY-001# " is streptomyces, Guangdong Province's microbial bacteria is preserved on March 24th, 2017
Kind collection, deposit number are GDMCC No.:60152, and preservation address is that the province of GuangZhou, China city martyr Road 100 is micro-
Biological five building, institute's laboratory building.
The embodiment 2 a kind of preparation and application of the liquid fertilizer for promoting root growth
It is a kind of promote root growth liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. it is few that multiple 0.1 part of phthalein nucleic acid, shell the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Sugared 0.1 part, 20 parts of calcium ammonium nitrate, amino acid 1 part, 17 parts of potassium dihydrogen phosphate, 11 parts of magnesium sulfate, microelement 1 part of (boric acid, chela
Close zinc, chelated iron, chelating molybdenum, each 0.2 part of chelated copper) obtain liquid fertilizer.
The embodiment 3 a kind of preparation and application of the liquid fertilizer for promoting root growth
It is a kind of promote root growth liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. it is few that multiple 0.5 part of phthalein nucleic acid, shell the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Sugared 0.3 part, 23 parts of calcium ammonium nitrate, 4 parts of amino acid, 19 parts of potassium dihydrogen phosphate, 11 parts of magnesium sulfate, microelement 1 part of (boric acid, chela
Close zinc, chelated iron, chelating molybdenum, each 0.2 part of chelated copper) obtain liquid fertilizer；Wherein B, Mg, Zn, Fe, Mn are respectively derived from boron
Acid, magnesium sulfate, chelated zinc, chelated iron, Chelated Manganese.
The embodiment 4 a kind of preparation and application of the liquid fertilizer for promoting root growth
It is a kind of promote root growth liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. it is few that multiple 0.7 part of phthalein nucleic acid, shell the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Sugared 0.6 part, 27 parts of calcium ammonium nitrate, 7 parts of amino acid, 22 parts of potassium dihydrogen phosphate, 15 parts of magnesium sulfate, microelement 6 parts of (boric acid, chela
Close zinc, chelated iron, chelating molybdenum, each 1.2 parts of chelated copper).
The embodiment 5 a kind of preparation and application of the liquid fertilizer for promoting root growth
It is a kind of promote root growth liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；Liquid
There are 200,000,000 viable bacterias for every milliliter in body strain；The ratio of liquid spawn and culture medium is 165g:250L；
S3. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S5. multiple 2 parts of phthalein nucleic acid, chitosan oligosaccharide the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
1.5 parts, 32 parts of calcium ammonium nitrate, 2 parts of amino acid 1,27 parts of potassium dihydrogen phosphate, 20 parts of magnesium sulfate, microelement 10 parts of (boric acid, chela
Close zinc, chelated iron, chelating molybdenum, each 2 parts of chelated copper).
The comparative example 1 a kind of preparation and application of the liquid fertilizer for promoting root growth
It is a kind of promote root growth liquid fertilizer preparation method the following steps are included:
S1. it prepares culture medium: after taking 1000g soybeans soaking 10h, being put into fiberizer and wear into soya-bean milk, filter off bean dregs, will filter
Liquid is placed in clean fermenter, after white granulated sugar then is added into filtrate, water is added to be settled to concentration 100g/ sugared in culture medium
L, pH 7.5；
S2. it ferments: opening oxygen pump, be continually fed into oxygen into fermenter during the fermentation, and according in fermenter
Independent Slurry bubble situation, is added suitable defoaming agent.Fermenter is covered with ventilated membrane, in order to avoid falling into sundries, is held at 25 DEG C
After supervention ferment 7 days, fermentation liquid is obtained；
S3. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains active liquid fertilizer substrate preparation.
S4. it is few that multiple 0.7 part of phthalein nucleic acid, shell the configuration of liquid fertilizer: are added into 1000 parts of active liquid fertilizer substrate preparations
Sugared 0.6 part, 27 parts of calcium ammonium nitrate, 7 parts of amino acid, 38 parts of potassium dihydrogen phosphate, 15 parts of magnesium sulfate, 6 parts of boric acid.
Promote the application effect appraisal of the liquid fertilizer of root growth:
Nutrient needed for liquid fertilizer can quickly supplement chili growth promotes capsicum root growth.For verifying present invention system
The standby liquid fertilizer actual effect that large-scale demonstration is applied on capsicum, applicant's selection have carried out test on beauty green pepper and have shown
Model.
1 materials and methods
1.1 are located in Zixing City beauty green pepper base for examination place 1, and 5 mu of testing ground area, soil fertility level is more equal
It is even.
It is located in the small tomato base in Jianning city for examination place 2,5 mu of testing ground area, soil fertility level is more uniform.
1.2 for studying 1 beauty green pepper of object, bar line-spacing 0.6m*0.6m, the growing way golden mean of the Confucian school and consistent.
For studying the small tomato of object 2, bar line-spacing 0.6m*0.6m, the growing way golden mean of the Confucian school and consistent.
1.3 for trying the liquid fertilizer that fertilizer is promotion root development made from embodiment 2~5, and product forms are liquid.
1.4 it is each for examination place carry out 2 test process:
1. experimental group: every month, which pours, after plantation applies once for trying fertilizer, 500mL/ mus each, dilutes 200 times.
2. comparative example group: being carried out every time with the chemical fertilizer same time prepared by the comparative example 1 of same experimental group equivalent per acre, other are applied
The same processing of fertile measure is 1..
Experimental group and control group, comparative example group are managed by local conventional cultivation technology.Each processing is tested with per acre
For cell, each processing randomly selects 1 plant of plant, is repeated 3 times, and management is consistent, observes strain body growing state, root amount and root class group
At acquiring average value.
2 results and analysis
Influence of 2.1 different disposals to root system
As shown in Table 1, the liquid fertilizer that prepared by the present embodiment 2~5 increases compared to total root item number of the comparative example group to beauty green pepper
Add 35%, total radical amount of small tomato has not been changed compared to comparative example group, while prepared by the present invention compared to comparative example group
Liquid fertilizer increases significantly to the Root Distribution and root class group Chengdu relative comparison group of beauty green pepper.However, the liquid fertilizer
Substantive effect is not played to the root amount of for example small tomato of other vegetables and fruits, Root Distribution and root class group Chengdu.As it can be seen that of the invention
Liquid fertilizer has selectivity to the strong plantlets and rootage of different vegetables and fruits, the most significant to the strong plantlets and rootage effect of beauty green pepper.
The root growth situation result of 1 different vegetable different disposal of table
The influence of 2.2 pairs of body of rod nutrient growth
As shown in Table 2, which has good facilitation to the nutrient growth of beauty green pepper, is conducive to plant nutrition
Accumulation.Wherein compared with comparative example group, beauty's green pepper length increases 29.6%, and crown diameter increases 36.5%, however, the liquid
Fertilizer only has more small effect to the plant strain growth of small tomato.As it can be seen that liquid fertilizer of the invention is strong to taking root for beauty green pepper
Seedling has very significant effect, to different vegetables and fruits selectivity with higher.
The plant height of the different vegetables and fruits different disposals of table 2, crown diameter situation statistics
| Processing | Plant height (m) | Crown diameter (m) |
| Beauty's green pepper experimental group | 0.92 | 0.71 |
| Beauty's green pepper comparative example group | 0.71 | 0.52 |
| Small tomato experimental group | 1.22 | 0.68 |
| Small tomato comparative example group | 1.13 | 0.68 |
The above description is only a preferred embodiment of the present invention, is not intended to restrict the invention, for those skilled in the art
For member, the invention may be variously modified and varied.All within the spirits and principles of the present invention, it is made it is any modification,
Equivalent replacement, improvement etc. should be included within scope of the invention.
Claims (10)
1. a kind of liquid fertilizer for promoting root growth, which is characterized in that the liquid fertilizer is by following material composition: active liquid
1000 parts of body fertilizer substrate preparation, multiple 0.1 ~ 2 part of phthalein nucleic acid, 0.1 ~ 1.5 part of chitosan oligosaccharide, 20 ~ 32 parts of calcium ammonium nitrate, amino acid 1 ~ 12
Part, 17 ~ 27 parts of potassium dihydrogen phosphate, 9 ~ 20 parts of magnesium sulfate, 1 ~ 10 part of microelement；Total nitrogen content in the liquid fertilizer >=
2.8mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter >=120g/L.
2. promoting the liquid fertilizer of root growth according to claim 1, which is characterized in that the liquid fertilizer is by following object
Matter composition: 1000 parts of active liquid fertilizer substrate preparation, multiple 0.5 ~ 1 part of phthalein nucleic acid, 0.3 ~ 0.9 part of chitosan oligosaccharide, calcium ammonium nitrate 23 ~ 30
Part, 4 ~ 10 parts of amino acid, 19 ~ 25 parts of potassium dihydrogen phosphate, 12 ~ 17 parts of magnesium sulfate, 4 ~ 8 parts of microelement；In the liquid fertilizer
Total nitrogen content >=2.8mg/g, amino nitrogen content >=0.4mg/g, the content of organic matter >=120g/L.
3. promoting the liquid fertilizer of root growth according to claim 1, which is characterized in that the liquid fertilizer is by following object
Matter composition: 1000 parts of active liquid fertilizer substrate preparation, multiple 0.7 part of phthalein nucleic acid, 0.6 part of chitosan oligosaccharide, 27 parts of calcium ammonium nitrate, amino acid
7 parts, 22 parts of potassium dihydrogen phosphate, 15 parts of magnesium sulfate, 6 parts of microelement；Total nitrogen content >=2.8mg/g, ammonia in the liquid fertilizer
Base nitrogen content >=0.4mg/g, the content of organic matter >=120g/L.
4. promoting the liquid fertilizer of root growth according to claim 1, which is characterized in that the active liquid fertilizer substrate system
Agent is obtained by streptomyces bacterial strain " TY-001# " fermentation；
The culture medium of streptomyces bacterial strain " TY-001# " are as follows: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, beaten
Slurry, filtering；Then after sugar being added into filtrate, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
The streptomyces bacterial strain " TY-001# " is preserved in Guangdong Province's Culture Collection on March 24th, 2017, point
Class is named as Streptomyces., and deposit number is GDMCC No.:60152, and preservation address is Guangzhou, Guangdong Province, China city
Martyr Road 100 five building, Institute of Micro-biology, province laboratory building.
5. promoting the liquid fertilizer of root growth according to claim 1, which is characterized in that N, P, K in the liquid fertilizer
Ratio be 1.5 ~ 3:1:1 ~ 2, preferably 8:5:7.
6. promoting the liquid fertilizer of root growth according to claim 1, which is characterized in that calcium in the liquid fertilizer, magnesium,
The ratio between phosphorus is 1 ~ 2:1:1 ~ 2, preferably 5:3:5.
7. according to claim 1 promote root growth liquid fertilizer, which is characterized in that the microelement contain boron,
Zinc, copper, molybdenum, iron.
8. a kind of preparation method for the liquid fertilizer for promoting root growth, which comprises the following steps:
S1. it prepares culture medium: after taking soybean to be impregnated or dregs of beans being taken to carry out pre fermentation, being beaten, filtered；Then toward filter
After sugar is added in liquid, water is added to be settled to concentration >=100g/L sugared in culture medium, pH is 7.4 ~ 7.6；
S2. it is inoculated with: taking the liquid spawn of streptomyces bacterial strain " TY-001# ", be inoculated into culture medium, stir evenly；
S3. it ferments: being continually fed into oxygen into culture medium during the fermentation, at 20 ~ 28 DEG C after continuing fermentation 7 ~ 8 days, obtain
To fermentation liquid；
S4. it terminates fermentation: fermentation liquid loading sealing being stood 15 days, obtains the active liquid fertilizer substrate preparation；
S5. the configuration of liquid fertilizer: toward the active liquid fertilizer substrate preparation add multiple phthalein nucleic acid, chitosan oligosaccharide, calcium ammonium nitrate,
Amino acid, potassium dihydrogen phosphate, magnesium sulfate, microelement obtain the liquid fertilizer.
9. promoting the preparation method of the liquid fertilizer of root growth according to claim 8, which is characterized in that the activity liquid
Its amino-acid nitrogen content >=4.8g/L after body fertilizer substrate preparation is stored 15 days.
10. application of any one of claim 1 ~ 9 liquid fertilizer for promoting root growth during pepper planting.
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| CN201711427736.7A CN109956782A (en) | 2017-12-25 | 2017-12-25 | A kind of liquid fertilizer promoting root growth |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113354474A (en) * | 2021-07-14 | 2021-09-07 | 新疆格莱克农牧科技有限公司 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
| CN113912447A (en) * | 2021-10-27 | 2022-01-11 | 哈撒韦(青岛)生物科技有限公司 | Liquid urea formaldehyde slow release fertilizer and production method thereof |
-
2017
- 2017-12-25 CN CN201711427736.7A patent/CN109956782A/en not_active Withdrawn
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113354474A (en) * | 2021-07-14 | 2021-09-07 | 新疆格莱克农牧科技有限公司 | Agricultural and forestry crop foliar bacterial liquid and preparation method thereof |
| CN113912447A (en) * | 2021-10-27 | 2022-01-11 | 哈撒韦(青岛)生物科技有限公司 | Liquid urea formaldehyde slow release fertilizer and production method thereof |
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