CN113197224A - Synergistic compound for preventing and treating gray mold of strawberries and application thereof - Google Patents
Synergistic compound for preventing and treating gray mold of strawberries and application thereof Download PDFInfo
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- CN113197224A CN113197224A CN202110535765.5A CN202110535765A CN113197224A CN 113197224 A CN113197224 A CN 113197224A CN 202110535765 A CN202110535765 A CN 202110535765A CN 113197224 A CN113197224 A CN 113197224A
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- gray mold
- phenazine
- formamide
- serenadesoil
- kasugamycin
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/50—Isolated enzymes; Isolated proteins
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- Pest Control & Pesticides (AREA)
- Agronomy & Crop Science (AREA)
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Abstract
The invention relates to the field of pesticides, and particularly relates to a synergistic compound for preventing and treating gray mold of strawberry and application thereof, wherein the compound consists of biological bactericides Serenadesoil, phenazine-1-formamide, superfine alternaria alternata protein, seaweed essence, kasugamycin and magnolol, and the mass ratio of the biological bactericides Serenadesoil, phenazine-1-formamide, superfine alternaria alternata protein, seaweed essence, kasugamycin and magnolol is 3-6: 1-9: 0.5-2.5: 1-9: 1-3: 5-10. The synergistic compound can be used for the synergistic control of the gray mold of the strawberries, delays the drug resistance of germs and prolongs the lasting period.
Description
Technical Field
The invention relates to the field of pesticides, and particularly relates to a synergistic compound for preventing and treating gray mold of strawberries and application thereof.
Background
Strawberry gray mold is a disease caused by botrytis cinerea infection and occurring on strawberries. The strawberry flower organ, fruits, petioles and leaves are mainly damaged, and different parts have different symptoms. Leaf damage symptoms form 'V' -shaped yellow brown spots in old leaves at the early stage of disease attack, and the leaves are scorched and die with the disease aggravation. In order to realize effective prevention and control of the gray mold of the strawberry, chemical agents are mostly used in production, but the long-term use of a large amount of chemical agents not only pollutes the environment, but also easily causes drug resistance of germs. At present, botrytis cinerea in many areas has resistance to fludioxonil, boscalid, procymidone, carbendazim, diethofencarb and other medicaments, which increases difficulty for prevention and control of the botrytis cinerea. A novel synergistic compound needs to be developed to reduce the drug resistance of the botrytis cinerea so as to realize the effective prevention and control of the botrytis cinerea.
Disclosure of Invention
In order to solve the problems, the invention provides a biological bactericide synergistic compound for preventing and treating gray mold of strawberries and application thereof.
In order to achieve the purpose, the invention adopts the technical scheme that
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericide Serenadesoil, phenazine-1-formamide, superfine alternaria protein, seaweed extract, kasugamycin and magnolol.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 3-6: 1-9: 0.5-2.5: 1-9: 1-3: 5-10.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 3: 9:2.5: 9: 3:10.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 4.5: 5:1.5: 5: 2:7.5.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 6: 1:0.5: 1: 1:5.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 5: 5:2: 5:2:8.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 4: 5:1: 5:2:7.
Further, the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 6: 1:1: 1: 1:5.
The synergistic compound for preventing and treating the gray mold of the strawberries can be used for preparing a medicament for preventing and treating the gray mold of the strawberries.
The synergistic compound can be used for the synergistic control of the gray mold of the strawberries, delays the drug resistance of germs and prolongs the lasting period.
Detailed Description
The present invention will be described in detail with reference to specific examples. The following examples will assist those skilled in the art in further understanding the invention, but are not intended to limit the invention in any way. It should be noted that variations and modifications can be made by persons skilled in the art without departing from the spirit of the invention. All falling within the scope of the present invention.
Example 1
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenadesoil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol according to the mass ratio of 3mg: 9mg:2.5 mg: 9mg: 3mg: 10mg of the above-mentioned components are mixed together.
Example 2
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenadesoil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol in a mass ratio of 4.5 mg: 5mg:1.5 mg: 5mg:2mg to 7.5 mg.
Example 3
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenadesoil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol in a mass ratio of 6 mg: 1mg:0.5 mg: 1mg:1mg to 5 mg.
Example 4
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenadesoil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract and kasugamycin in a mass ratio of 5mg: 5mg, 2mg: 5mg to 2mg to 8 mg.
Example 5
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenadesoil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol in a mass ratio of 4 mg: 5mg: 1mg:5mg to 2mg to 7 mg.
Example 6
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenade soil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol in a mass ratio of 6 mg: 1mg: 1mg:1mg to 5 mg.
Comparative example 1
The compound is prepared from a biological bactericide Serenadesoil and phenazine-1-formamide according to the mass ratio of 3mg: 9mg to 2.5 mg.
Comparative example 2
The compound is prepared from a biological bactericide Serenadesoil, phenazine-1-formamide and superfine alternaria protein according to a mass ratio of 4.5 mg: 5mg:1.5 mg:5mg of the components are compounded.
Comparative example 3
The compound is prepared from a biological bactericide Serenadesoil, phenazine-1-formamide, superfine alternaria protein and seaweed extract in a mass ratio of 6 mg: 1mg:0.5 mg:1mg of the components are compounded.
Comparative example 4
The compound is prepared from biological bactericide Serenadesoil, phenazine-1-formamide, superfine alternaria protein, seaweed extract and kasugamycin in a mass ratio of 5mg: 5mg, 2mg: the 5mg to 2mg are compounded.
Comparative example 5
A synergistic compound for preventing and treating gray mold of strawberry is prepared from biological bactericides Serenade soil, phenazine-1-formamide, alternaria tenuissima protein, seaweed extract, kasugamycin and magnolol in a mass ratio of 3mg:3mg:3mg to 3mg, and the mixture is compounded according to the proportion of 3mg to 3 mg.
Indoor toxicity determination:
botrytiscineera is collected in the field and is subjected to indoor purification culture, and the test method refers to the agricultural industry standard of the people's republic of China. Inoculating the cultured botrytis cinerea to the surfaces of the strawberry fruits by adopting a living method, and culturing in a constant-temperature constant-humidity incubator at the temperature of 20-25 ℃ for 3-5 days; inspecting the area of lesion spots, preparing 500 times of solution by spraying, uniformly spraying once (about 2 ml) on the surface of each strawberry, calculating the percentage of inhibition of lesion spots growth, and determining the EC of each medicine by linear regression analysis between the probability of inhibition and the logarithm of the series concentration50The co-toxicity coefficient (CTC) of the combination is calculated by using the co-toxicity coefficient table, so as to evaluate the activity of the test drug on pathogenic bacteria.
As a result: the co-toxicity coefficients of the examples 1, 2, 3, 4, 5 and 6 are all significantly higher than those of the comparative examples 1, 2, 3, 4, 5 and the single agents, and the co-toxicity coefficient of the example 3 is the highest; the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 3: 9:2.5: 9: 3:10/4.5: 5:1.5: 5: 2:7.5/6: 1:0.5: 1: 1:5/5: 5:2: 5:2:8/4: 5:1: 5:2:7/6: 1:1: 1: 1:5, the composition shows good synergistic effect, and when the mass ratio of Serenadesoil to phenazine-1-formamide to Alternaria tenuissima protein to trehalamine is 6: 1:0.5: 1: the synergistic effect is best when the ratio is 1: 5. Compared with single agents and comparative examples 1, 2, 3, 4 and 5, the composition has the advantages of good control effect on the gray mold of the strawberry and long lasting period.
The foregoing description of specific embodiments of the present invention has been presented. It is to be understood that the present invention is not limited to the specific embodiments described above, and that various changes or modifications may be made by one skilled in the art within the scope of the appended claims without departing from the spirit of the invention. The embodiments and features of the embodiments of the present application may be combined with each other arbitrarily without conflict.
Claims (9)
1. A synergistic compound for preventing and treating gray mold of strawberry is characterized in that: the compound consists of biological bactericide Serenadesoil, phenazine-1-formamide, alternaria tenuis protein, seaweed extract, kasugamycin and magnolol.
2. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 3-6: 1-9: 0.5-2.5: 1-9: 1-3: 5-10.
3. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 3: 9:2.5: 9: 3:10.
4. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 4.5: 5:1.5: 5: 2:7.5.
5. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 6: 1:0.5: 1: 1:5.
6. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 5: 5:2: 5:2:8.
7. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 4: 5:1: 5:2:7.
8. The synergistic compound for controlling gray mold of strawberry as claimed in claim 1, wherein: the mass ratio of the biological bactericide Serenadesoil to the phenazine-1-formamide to the superfine alternaria protein to the seaweed extract to the kasugamycin to the magnolol is 6: 1:1: 1: 1:5.
9. The application of the synergistic compound for controlling the gray mold of the strawberries as claimed in any one of claims 1 to 8, wherein the synergistic compound comprises the following components in percentage by weight: can be used for preparing a medicament for preventing and treating gray mold of strawberry.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202110535765.5A CN113197224A (en) | 2021-05-17 | 2021-05-17 | Synergistic compound for preventing and treating gray mold of strawberries and application thereof |
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| CN202110535765.5A CN113197224A (en) | 2021-05-17 | 2021-05-17 | Synergistic compound for preventing and treating gray mold of strawberries and application thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113711833A (en) * | 2021-09-22 | 2021-11-30 | 李辰江 | Method for cultivating raspberries in open field |
| CN114391416A (en) * | 2022-02-25 | 2022-04-26 | 河北省农林科学院石家庄果树研究所 | Pear tree cultivation method for preventing pear tree trunk diseases |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113711833A (en) * | 2021-09-22 | 2021-11-30 | 李辰江 | Method for cultivating raspberries in open field |
| CN114391416A (en) * | 2022-02-25 | 2022-04-26 | 河北省农林科学院石家庄果树研究所 | Pear tree cultivation method for preventing pear tree trunk diseases |
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Application publication date: 20210803 |