CN118525850A - Sterilization composition for preventing and treating rust disease of mesona chinensis benth - Google Patents
Sterilization composition for preventing and treating rust disease of mesona chinensis benth Download PDFInfo
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- CN118525850A CN118525850A CN202410617995.XA CN202410617995A CN118525850A CN 118525850 A CN118525850 A CN 118525850A CN 202410617995 A CN202410617995 A CN 202410617995A CN 118525850 A CN118525850 A CN 118525850A
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- Prior art keywords
- honokiol
- rust
- mesona
- diniconazole
- compounded
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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- 239000000203 mixture Substances 0.000 title claims abstract description 22
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 title claims abstract description 21
- 241001646828 Platostoma chinense Species 0.000 title abstract description 15
- 230000001954 sterilising effect Effects 0.000 title abstract description 7
- 201000010099 disease Diseases 0.000 title description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title description 7
- 238000004659 sterilization and disinfection Methods 0.000 title description 4
- FVYXIJYOAGAUQK-UHFFFAOYSA-N honokiol Chemical compound C1=C(CC=C)C(O)=CC=C1C1=CC(CC=C)=CC=C1O FVYXIJYOAGAUQK-UHFFFAOYSA-N 0.000 claims abstract description 32
- BYTORXDZJWWIKR-UHFFFAOYSA-N Hinokiol Natural products CC(C)c1cc2CCC3C(C)(CO)C(O)CCC3(C)c2cc1O BYTORXDZJWWIKR-UHFFFAOYSA-N 0.000 claims abstract description 31
- VVOAZFWZEDHOOU-UHFFFAOYSA-N honokiol Natural products OC1=CC=C(CC=C)C=C1C1=CC(CC=C)=CC=C1O VVOAZFWZEDHOOU-UHFFFAOYSA-N 0.000 claims abstract description 31
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 23
- FBOUIAKEJMZPQG-BLXFFLACSA-N diniconazole-M Chemical compound C1=NC=NN1/C([C@H](O)C(C)(C)C)=C/C1=CC=C(Cl)C=C1Cl FBOUIAKEJMZPQG-BLXFFLACSA-N 0.000 claims abstract description 13
- 239000005783 Fluopyram Substances 0.000 claims abstract description 12
- KVDJTXBXMWJJEF-UHFFFAOYSA-N fluopyram Chemical compound ClC1=CC(C(F)(F)F)=CN=C1CCNC(=O)C1=CC=CC=C1C(F)(F)F KVDJTXBXMWJJEF-UHFFFAOYSA-N 0.000 claims abstract description 12
- 241001646834 Mesona Species 0.000 claims abstract description 9
- 239000003899 bactericide agent Substances 0.000 claims description 8
- 206010059866 Drug resistance Diseases 0.000 abstract description 9
- 230000035784 germination Effects 0.000 abstract description 7
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 230000006806 disease prevention Effects 0.000 abstract description 4
- 230000002401 inhibitory effect Effects 0.000 abstract description 4
- 239000004480 active ingredient Substances 0.000 abstract description 2
- 244000052616 bacterial pathogen Species 0.000 abstract description 2
- 230000003111 delayed effect Effects 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 241000249261 Uromyces sp. Species 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000012258 culturing Methods 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 230000005764 inhibitory process Effects 0.000 description 3
- 230000004763 spore germination Effects 0.000 description 3
- 239000000725 suspension Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 241000219357 Cactaceae Species 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 235000001484 Trigonella foenum graecum Nutrition 0.000 description 2
- 244000250129 Trigonella foenum graecum Species 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000000447 pesticide residue Substances 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 235000001019 trigonella foenum-graecum Nutrition 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 1
- 241001530056 Athelia rolfsii Species 0.000 description 1
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 1
- 241000192125 Firmicutes Species 0.000 description 1
- 241000223218 Fusarium Species 0.000 description 1
- 241000223221 Fusarium oxysporum Species 0.000 description 1
- 241000233732 Fusarium verticillioides Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Chemical compound OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000207923 Lamiaceae Species 0.000 description 1
- 241000243785 Meloidogyne javanica Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 206010039509 Scab Diseases 0.000 description 1
- 241000221696 Sclerotinia sclerotiorum Species 0.000 description 1
- 241000191940 Staphylococcus Species 0.000 description 1
- 241000194019 Streptococcus mutans Species 0.000 description 1
- 208000025865 Ulcer Diseases 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- 210000001015 abdomen Anatomy 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000001914 calming effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 244000053095 fungal pathogen Species 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 235000015110 jellies Nutrition 0.000 description 1
- 239000008274 jelly Substances 0.000 description 1
- 238000012417 linear regression Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 239000012452 mother liquor Substances 0.000 description 1
- 210000005036 nerve Anatomy 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 238000007790 scraping Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- 239000003206 sterilizing agent Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 231100000397 ulcer Toxicity 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 230000001018 virulence Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/64—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with three nitrogen atoms as the only ring hetero atoms
- A01N43/647—Triazoles; Hydrogenated triazoles
- A01N43/653—1,2,4-Triazoles; Hydrogenated 1,2,4-triazoles
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of rust disease prevention and treatment of mesona chinensis benth, and particularly relates to a sterilizing composition for rust disease prevention and treatment of mesona chinensis benth. The active ingredients of the bactericidal composition are compounded by honokiol and diniconazole. According to the invention, honokiol and diniconazole or fluopyram are compounded, so that the drug resistance risk during independent use of honokiol can be overcome, and the generation of pathogenic bacteria drug resistance can be delayed. In addition, when the honokiol and diniconazole or fluopyram are compounded, the synergistic effect is shown on inhibiting the germination of the mesona blume rust spores, and compared with single components, the synergistic effect on preventing and treating the mesona blume rust can be improved.
Description
Technical Field
The invention belongs to the technical field of rust disease prevention and treatment of mesona chinensis benth, and particularly relates to a sterilizing composition for rust disease prevention and treatment of mesona chinensis benth.
Background
Mesona chinensis (Mesona hinensis benth.) is also known as mesona chinensis, cactus jelly, mesona chinensis, cactus, etc., and is an herbaceous perennial root plant of mesona in Labiatae. The disease types of the Guangxi mesona chinensis benth mainly comprise: wilt (Fusarium oxysporum) damaging whole plants, root knot nematode (Meloidog incognita) damaging roots, anthracnose (Colletotrichum gloeosporioides) damaging leaves and roots, viral disease (CMV) damaging whole plants, stem basal rot (Fusarium moniliforme) damaging stem bases, rust damaging leaves and young shoots (Uromyces sp.).
In the process of preventing and treating fungal diseases, the bactericide plays a very important role and has the advantages of quick response, accurate target, convenient application and the like. However, there are other disadvantages such as the risk of drug resistance caused by long-term use of a bactericide of a certain composition. In the actual production process, the control effect can be improved by increasing the application amount of the bactericide, but the development of drug resistance can be aggravated, and the pesticide residue is overproof, so that the health of people is endangered.
Honokiol (CAS: 35354-74-6) is a yellowish brown to pale yellow or white powder. In terms of antibacterial action, honokiol has remarkable antibacterial activity on gram-positive bacteria, acid-resistant bacteria and filamentous fungi, has more remarkable antibacterial action on streptococcus mutans and has the strongest inhibitory action on staphylococcus. Clinically, honokiol is mainly used for resisting fungi and ulcers, eliminating chest and abdomen fullness and calming central nerves and the like. In addition, CN2021110454082.7 discloses the use of honokiol as an agricultural fungicide. In the specification, the molecular formula and the structural formula of honokiol are disclosed, and meanwhile, the honokiol can effectively prevent and treat a plurality of plant diseases such as sclerotinia sclerotiorum, banded sclerotial blight, gray mold, scab, anthracnose, bakanae disease, gummy stem blight, fusarium wilt, verticillium wilt, rice blast and the like. Thus, honokiol has the potential of developing into agricultural bactericides. However, the honokiol of a single component has a certain drug resistance risk, so that the development of a compound medicament of the honokiol is necessary.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a sterilizing composition for preventing and treating rust disease of mesona chinensis benth, which aims to solve the problem that drug resistance is easy to cause when a sterilizing agent with a certain component is used for a long time in the background technology. In the actual production process, the control effect can be improved by increasing the application amount of the bactericide, but the development of drug resistance is aggravated, and the problem that the pesticide residue exceeds standard and endangers the health of people is also caused.
In order to achieve the above purpose, the present invention provides the following technical solutions:
The active ingredients of the bactericidal composition are compounded by honokiol and diniconazole.
The effect is better, and the mass ratio of honokiol to diniconazole is 1-16:24-1.
The effect is better, and the mass ratio of honokiol to diniconazole is 1:4.
The application also provides another sterilization composition, and the effective components of the sterilization composition are compounded by honokiol and fluopyram.
The effect is better, and the mass of honokiol and fluopyram is 1-15:9-1.
The effect is better, and the mass of honokiol and fluopyram is 1:9.
The application also provides application of the bactericidal composition in preventing and treating mesona chinensis rust or in preparing a bactericide for preventing and treating mesona chinensis rust.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the invention, honokiol and diniconazole or fluopyram are compounded, so that the drug resistance risk during independent use of honokiol can be overcome, and the generation of pathogenic bacteria drug resistance can be delayed.
(2) When the honokiol and diniconazole or fluopyram are compounded, the synergistic effect is shown on inhibiting the germination of mesona blume rust spores, and compared with single components, the synergistic effect on preventing and treating mesona blume rust can be improved.
Detailed Description
The following description of the embodiments of the present invention will be apparent from the description of the embodiments of the present invention, which is provided in part, but not in whole. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: indoor biological Activity assay
1. Test strain
Scraping rust spores from mesona chinensis benth leaves to prepare spore suspension, inoculating the spore suspension onto indoor potted mesona chinensis benth leaves, culturing in dark at 18 ℃ for 48 hours, and culturing under the conditions of 20 ℃ and 12 h/12 h of illumination. Fresh summer spores of rust (Uromyces sp.) are collected, the spore concentration is adjusted to about 30 spores per field under a 40-fold microscope with deionized water, and a 0.5% dextrose solution is added.
2. Test agent
98 Percent of honokiol, 95 percent of diniconazole technical product 96% fluopyram original drug
After the test reagent is completely dissolved, diluting with 0.1% Tween-80 aqueous solution to prepare single-dose mother liquor, setting a plurality of groups of proportions, and setting 5 mass concentration gradients for each single dose and each proportion of the mixture according to an equal proportion method. The preparation can be prepared for use at present.
3. Test method (refer to "NY/T1156.1-2006. Criterion for laboratory biological tests of pesticides section 1: concave slide method for tests for inhibiting spore germination of pathogenic fungi
Mixing spore suspension and medicinal liquid in equal volume, sucking 150 μl of the mixture with microsampler, placing on a culture dish with shallow water, and culturing in incubator at 20deg.C under illumination for 12 hr/darkness for 12 hr. Each treatment was set up in 3 replicates and a treatment with 0.1% tween-80 in water was set up as a blank.
And observing and counting the germination condition of 1-time spores every 4 hours, and checking the germination condition of each treated spore when the germination rate of the blank control spores reaches more than 90 percent. Each treatment was repeated with random observation of 3 fields, investigation of 300 total spores, recording the number of germination and total spores, respectively, wherein the spore bud tube length was greater than the short radius of the spores as germination. Based on the survey data, the relative inhibition of spore germination was calculated for each treatment.
4. Data analysis
And (3) carrying out data statistical analysis by adopting DPS software, carrying out linear regression by taking the logarithmic value of the concentration of the bactericide as x and the corresponding mechanical value of the spore germination relative inhibition rate as y to obtain a virulence regression equation and the concentration EC 50 value of the medicament in the inhibition of the mesona blume on the rust bacteria, and calculating a co-toxicity coefficient (CTC) according to a grand cloud Peel method.
5. Measurement results
The synergy of the agents was evaluated based on the calculated co-toxicity coefficient (CTC), CTC.ltoreq.80 being antagonism, CTC.ltoreq.120 being additive, CTC.ltoreq.120 being synergy, the results being shown in tables 1-3.
As can be seen from Table 2, after the honokiol and diniconazole are compounded, the mass ratio is 1-16: the co-toxicity coefficient of the fenugreek rust bacteria (Uromyces sp.) in 24-1 is more than 120, and the synergistic effect is shown.
As shown in Table 3, after the honokiol and the fluopyram are compounded, the mass ratio is 1-15: the co-toxicity coefficient of the fenugreek rust bacteria (Uromyces sp.) in 9-1 is more than 120, and the synergistic effect is shown.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (7)
1. The bactericidal composition is characterized in that the effective components of the bactericidal composition are compounded by honokiol and diniconazole.
2. The bactericidal composition of claim 1, wherein the mass ratio of honokiol to diniconazole is 1-16:24-1.
3. The bactericidal composition of claim 1, wherein the mass ratio of honokiol to diniconazole is 1:4.
4. The bactericidal composition is characterized in that the effective components of the bactericidal composition are compounded by honokiol and fluopyram.
5. The bactericidal composition of claim 4, wherein the mass of honokiol and fluopyram is 1-15:9-1.
6. The bactericidal composition of claim 4, wherein the mass of honokiol and fluopyram is 1:9.
7. Use of the bactericidal composition of any of claims 1-6 for controlling mesona blume rust or for preparing a bactericide for controlling mesona blume rust.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410617995.XA CN118525850A (en) | 2024-05-17 | 2024-05-17 | Sterilization composition for preventing and treating rust disease of mesona chinensis benth |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202410617995.XA CN118525850A (en) | 2024-05-17 | 2024-05-17 | Sterilization composition for preventing and treating rust disease of mesona chinensis benth |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN118525850A true CN118525850A (en) | 2024-08-23 |
Family
ID=92389878
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202410617995.XA Pending CN118525850A (en) | 2024-05-17 | 2024-05-17 | Sterilization composition for preventing and treating rust disease of mesona chinensis benth |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN118525850A (en) |
-
2024
- 2024-05-17 CN CN202410617995.XA patent/CN118525850A/en active Pending
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