CN118383367A - Pesticide composition for preventing and treating pepper anthracnose - Google Patents
Pesticide composition for preventing and treating pepper anthracnose Download PDFInfo
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- CN118383367A CN118383367A CN202410500177.1A CN202410500177A CN118383367A CN 118383367 A CN118383367 A CN 118383367A CN 202410500177 A CN202410500177 A CN 202410500177A CN 118383367 A CN118383367 A CN 118383367A
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- asarone
- pepper anthracnose
- pepper
- pesticide composition
- anthracnose
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- 235000002566 Capsicum Nutrition 0.000 title claims abstract description 46
- 239000006002 Pepper Substances 0.000 title claims abstract description 42
- 235000016761 Piper aduncum Nutrition 0.000 title claims abstract description 42
- 235000017804 Piper guineense Nutrition 0.000 title claims abstract description 42
- 235000008184 Piper nigrum Nutrition 0.000 title claims abstract description 42
- 239000000203 mixture Substances 0.000 title claims abstract description 29
- 239000000575 pesticide Substances 0.000 title claims abstract description 16
- 244000203593 Piper nigrum Species 0.000 title 1
- 241000722363 Piper Species 0.000 claims abstract description 42
- RKFAZBXYICVSKP-AATRIKPKSA-N alpha-asarone Chemical compound COC1=CC(OC)=C(\C=C\C)C=C1OC RKFAZBXYICVSKP-AATRIKPKSA-N 0.000 claims abstract description 37
- NCXMLFZGDNKEPB-FFPOYIOWSA-N natamycin Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C[C@@H](C)OC(=O)/C=C/[C@H]2O[C@@H]2C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 NCXMLFZGDNKEPB-FFPOYIOWSA-N 0.000 claims abstract description 18
- 229960003255 natamycin Drugs 0.000 claims abstract description 18
- 235000010298 natamycin Nutrition 0.000 claims abstract description 18
- 239000004311 natamycin Substances 0.000 claims abstract description 18
- 229930182764 Polyoxin Natural products 0.000 claims abstract description 16
- YEBIHIICWDDQOL-YBHNRIQQSA-N polyoxin Polymers O[C@@H]1[C@H](O)[C@@H](C(C=O)N)O[C@H]1N1C(=O)NC(=O)C(C(O)=O)=C1 YEBIHIICWDDQOL-YBHNRIQQSA-N 0.000 claims abstract description 16
- 201000010099 disease Diseases 0.000 claims abstract description 15
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 15
- 239000004480 active ingredient Substances 0.000 claims abstract description 3
- 239000003814 drug Substances 0.000 claims description 13
- 230000000361 pesticidal effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 20
- 230000005764 inhibitory process Effects 0.000 abstract description 12
- 206010059866 Drug resistance Diseases 0.000 abstract description 5
- 244000052616 bacterial pathogen Species 0.000 abstract description 5
- 230000002195 synergetic effect Effects 0.000 abstract description 5
- 244000000004 fungal plant pathogen Species 0.000 abstract description 4
- 231100000820 toxicity test Toxicity 0.000 abstract description 4
- -1 asarone compound Chemical class 0.000 abstract description 3
- 231100000419 toxicity Toxicity 0.000 description 8
- 230000001988 toxicity Effects 0.000 description 8
- 239000003795 chemical substances by application Substances 0.000 description 6
- 230000000844 anti-bacterial effect Effects 0.000 description 5
- 238000012360 testing method Methods 0.000 description 5
- 240000008574 Capsicum frutescens Species 0.000 description 4
- 241001529387 Colletotrichum gloeosporioides Species 0.000 description 4
- 239000003899 bactericide agent Substances 0.000 description 4
- 239000001390 capsicum minimum Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000222199 Colletotrichum Species 0.000 description 3
- 241000222239 Colletotrichum truncatum Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- FYRHTIWFKXZWAD-UHFFFAOYSA-N Sarisan Chemical compound C1=C(CC=C)C(OC)=CC2=C1OCO2 FYRHTIWFKXZWAD-UHFFFAOYSA-N 0.000 description 3
- 239000007864 aqueous solution Substances 0.000 description 3
- 230000001717 pathogenic effect Effects 0.000 description 3
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 3
- 229920000053 polysorbate 80 Polymers 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 230000001018 virulence Effects 0.000 description 3
- 241000223600 Alternaria Species 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- TWFZGCMQGLPBSX-UHFFFAOYSA-N carbendazim Chemical compound C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000013329 compounding Methods 0.000 description 2
- 229940079593 drug Drugs 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 239000010413 mother solution Substances 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- STMIIPIFODONDC-UHFFFAOYSA-N 2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1-yl)hexan-2-ol Chemical compound C=1C=C(Cl)C=C(Cl)C=1C(O)(CCCC)CN1C=NC=N1 STMIIPIFODONDC-UHFFFAOYSA-N 0.000 description 1
- 229920001817 Agar Polymers 0.000 description 1
- 235000006578 Althaea Nutrition 0.000 description 1
- 206010003210 Arteriosclerosis Diseases 0.000 description 1
- 239000005730 Azoxystrobin Substances 0.000 description 1
- 241000193738 Bacillus anthracis Species 0.000 description 1
- 241001133184 Colletotrichum agaves Species 0.000 description 1
- 241000606071 Colletotrichum nigrum Species 0.000 description 1
- 241001001916 Colletotrichum scovillei Species 0.000 description 1
- 241001639768 Colletotrichum siamense Species 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 241000288105 Grus Species 0.000 description 1
- 244000130592 Hibiscus syriacus Species 0.000 description 1
- 241000755266 Kathetostoma giganteum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 230000003042 antagnostic effect Effects 0.000 description 1
- 208000011775 arteriosclerosis disease Diseases 0.000 description 1
- WFDXOXNFNRHQEC-GHRIWEEISA-N azoxystrobin Chemical compound CO\C=C(\C(=O)OC)C1=CC=CC=C1OC1=CC(OC=2C(=CC=CC=2)C#N)=NC=N1 WFDXOXNFNRHQEC-GHRIWEEISA-N 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001556 benzimidazoles Chemical class 0.000 description 1
- 239000006013 carbendazim Substances 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- FQKUGOMFVDPBIZ-UHFFFAOYSA-N flusilazole Chemical compound C=1C=C(F)C=CC=1[Si](C=1C=CC(F)=CC=1)(C)CN1C=NC=N1 FQKUGOMFVDPBIZ-UHFFFAOYSA-N 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000002460 imidazoles Chemical class 0.000 description 1
- 150000002466 imines Chemical class 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- OXOKTPUZOHPXSA-UHFFFAOYSA-L manganese(2+);n-propyl-n-[2-(2,4,6-trichlorophenoxy)ethyl]imidazole-1-carboxamide;dichloride Chemical class Cl[Mn]Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl.C1=CN=CN1C(=O)N(CCC)CCOC1=C(Cl)C=C(Cl)C=C1Cl OXOKTPUZOHPXSA-UHFFFAOYSA-L 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 208000013435 necrotic lesion Diseases 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000003068 static effect Effects 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 230000002110 toxicologic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 150000003852 triazoles Chemical class 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/24—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms
- A01N43/26—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings
- A01N43/28—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings with two hetero atoms in positions 1,3
- A01N43/30—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with two or more hetero atoms five-membered rings with two hetero atoms in positions 1,3 with two oxygen atoms in positions 1,3, condensed with a carbocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N47/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid
- A01N47/08—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom not being member of a ring and having no bond to a carbon or hydrogen atom, e.g. derivatives of carbonic acid the carbon atom having one or more single bonds to nitrogen atoms
- A01N47/10—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof
- A01N47/12—Carbamic acid derivatives, i.e. containing the group —O—CO—N<; Thio analogues thereof containing a —O—CO—N< group, or a thio analogue thereof, neither directly attached to a ring nor the nitrogen atom being a member of a heterocyclic ring
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Plant Pathology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Agronomy & Crop Science (AREA)
- Health & Medical Sciences (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Mycology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pepper disease control, and particularly relates to a pesticide composition for controlling pepper anthracnose. A pesticide composition for preventing and treating pepper anthracnose comprises active ingredients of asarone and polyoxin or natamycin according to the proportion of 1-150:150-1 by mass ratio. According to the invention, on the basis of the good inhibition activity of the conventional asarone on plant pathogenic fungi, the asarone compound formula with good inhibition effect on pepper anthracnose is screened. Indoor combined toxicity tests show that when asarone and polyoxin or natamycin are compounded according to a certain mass ratio, the co-toxicity coefficient of pathogenic bacteria of pepper anthracnose is above 120, and the synergistic effect is shown, so that the control effect on pepper anthracnose can be improved. In addition, compared with single asarone, the pesticide composition can reduce the drug resistance risk, and provides technical support and scientific guidance for pertinently preventing and controlling pepper anthracnose.
Description
Technical Field
The invention belongs to the technical field of pepper disease control, and particularly relates to a pesticide composition for controlling pepper anthracnose.
Background
The capsicum is a annual or limited perennial herb plant, and the vitamin C content in vegetables is the first position, so that arteriosclerosis can be effectively prevented, and cholesterol can be reduced. The planting area and the yield of the capsicum are famous and forepart in vegetable crops, and capsicum becomes an important dominant industry in some provinces in China. However, the long-time large-area planting, especially the continuous cropping, causes the frequent occurrence of pepper diseases, affects the yield and the quality, and causes great economic loss for the grower.
Anthracnose is a main disease on capsicum, and causes yield reduction of more than 30% all year round in main production areas in China. The pathogenic bacteria of the pepper anthracnose are complex in species and mainly comprise colletotrichum gloeosporioides (Colletotrichum gloeosporioides), black colletotrichum (Colletotrichum nigrum), colletotrichum truncatum (Colletotrichum truncatum), colletotrichum steganensis (Colletotrichum scovillei), and colletotrichum siamensis (Colletotrichum siamense). Typical anthracnose symptoms usually occur on ripe pepper fruits as concave necrotic lesions with black concentric rings. Anthracnose can also infect pepper seeds, which, after being infected, cause the germinated seedlings to die before or after emergence. In addition, the leaves can also be infected, and the leaves in the seedling stage are in a static state after being infected by pathogenic bacteria, so that the leaves show disease symptoms when being aged, and the leaves are used as main pathogens of fruits and leaves in the infection fruiting stage.
Regarding the control of pepper anthracnose, biological control of pepper anthracnose is still in the primary stage, and excellent disease-resistant varieties are not cultivated. At present, chemical control is a main means for controlling pepper anthracnose, such as carbendazim of benzimidazoles, flusilazole and hexaconazole of triazoles, procymidone of imines, azoxystrobin of methoxy acrylic esters, prochloraz manganese salts of imidazoles and the like. However, depending on a single-component bactericide, pathogenic strains can generate drug resistance, and the control effect of the medicament is affected, so that the difficulty of disease management is increased.
Different bactericide components are compounded, and a compounding scheme with a synergistic effect is screened, so that the control effect on pathogenic strains can be improved, and the generation of drug resistance of pathogenic strains can be delayed. Therefore, the screening of the compounding method with a strong inhibition effect on the pepper anthracnose can provide technical support and scientific guidance for pertinently preventing and controlling the pepper anthracnose, and has very important significance.
Asarone (Sarisan), CAS accession number: 18607-93-7, the molecular structural formula is as follows:
the patent of the issued publication No. CN100475035C discloses the sterilization application of asarone for preventing and treating plant diseases caused by plant pathogenic bacteria, and the asarone has good inhibition activity on plant pathogenic fungi by the form of pesticide bactericide preparation prepared from the asarone and field efficacy test. As a bactericide, asarone is high-efficiency and low-toxicity, and meets the requirements of chemical control of plant diseases.
The information disclosed in this background section is only for enhancement of understanding of the general background of the invention and should not be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art.
Disclosure of Invention
The invention aims to provide a pesticide composition for preventing and treating pepper anthracnose, which can reduce the drug resistance risk compared with single use of asarone and can provide technical support and scientific guidance for pertinently preventing and treating pepper anthracnose.
In order to achieve the above purpose, the present invention provides the following technical solutions:
A pesticide composition for preventing and treating pepper anthracnose comprises active ingredients of asarone and polyoxin or natamycin according to the proportion of 1-150:150-1 by mass ratio.
The effect is better, and the mass ratio of the asarone to the polyoxin is 1-30:8-1.
The effect is better, and the mass ratio of asarone to natamycin is 1-4:27-1.
The effect is better, and the mass ratio of asarone to natamycin is 1:18.
The invention also provides application of the bactericidal pesticide composition in preventing and treating pepper diseases or preparing a medicament for preventing and treating pepper diseases.
More specifically, the pepper disease is pepper anthracnose.
Compared with the prior art, the invention has the following beneficial effects:
(1) According to the invention, on the basis of the good inhibition activity of the conventional asarone on plant pathogenic fungi, the asarone compound formula with good inhibition effect on pepper anthracnose is screened. Indoor combined toxicity tests show that when asarone and polyoxin or natamycin are compounded according to a certain mass ratio, the co-toxicity coefficient of pathogenic bacteria of pepper anthracnose is above 120, and the synergistic effect is shown, so that the control effect on pepper anthracnose can be improved.
(2) Compared with single asarone, the pesticide composition of the invention can reduce the drug resistance risk and provide technical support and scientific guidance for pertinently preventing and controlling pepper anthracnose.
Detailed Description
The following description of the embodiments of the present invention will be apparent from, and is intended to provide a thorough description of, the embodiments of the present invention, and not a complete description of, the embodiments of the present invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the present invention without making any inventive effort, are intended to fall within the scope of the present invention.
Examples: joint toxicity test of asarone
1. Test agent
98% Asarone, 99% polyoxin, 95% natamycin;
After the test reagent is completely dissolved, a mother solution of 10000mg/L is prepared by using a Tween-80 aqueous solution with the volume fraction of 0.1 percent. And (3) setting a plurality of groups of proportions, diluting a single-dose mother solution and each proportion of mixed agents into 5 mass concentration gradients by using a Tween-80 aqueous solution with the volume fraction of 0.1 percent, and preparing the medicaments at present.
2. Test strain
Anthracnose grub (Colletotrichum truncatum) isolated from pepper plants that are infected with anthracnose.
3. Culture medium
PDA medium: peeled potato 200g, glucose 20g, agar 17g, distilled water to 1000mL, natural pH.
4. Toxicity determination
The toxicity measurement is carried out on the mixed agent of asarone, polyoxin, natamycin and the mixture of asarone and polyoxin and the mixture of asarone and natamycin by adopting a hypha growth rate method.
1ML of the liquid medicine and 9mL of PDA culture medium cooled to about 50 ℃ are uniformly mixed, and poured into a culture dish with the diameter of 9cm to prepare a medicine-containing flat plate with corresponding concentration. The bacterial cake with the diameter of 6mm is obtained on the same circumference of the colony edge of the test bacterial strain after propagation by a puncher, inoculated to the center of a drug-containing flat plate containing different concentrations of drugs, one of the plates is covered with a plate cover and then placed in a constant temperature oven at 25 ℃ for culturing for 4d, the diameter of each treated colony is measured by a crisscross method, and the treatment of Tween-80 aqueous solution with the volume fraction of 0.1% is used as a control, and 5 repetitions are arranged for each concentration treatment. The inhibition of hypha growth by the different treatments was calculated.
And linearly regressing the corresponding hypha growth inhibition rate value with the fungicide concentration logarithmic value being x and the corresponding hypha growth inhibition rate value being y to obtain a virulence regression equation and a concentration EC 50 value in effective inhibition of the medicament on the tested strain.
5. Data analysis
And calculating a co-toxicity coefficient (CTC) according to a grand cloud peak method, and evaluating the synergy of the medicament according to the calculated co-toxicity coefficient (CTC), wherein CTC is less than or equal to 80 and is antagonistic, CTC is less than or equal to 120 and is additive, and CTC is more than or equal to 120 and is synergistic.
In the above formula: ati—the measured virulence index of the mixture; s- -EC 50 of standard medicament in mg/L; m- -EC 50 of the mixture in mg/L.
TTI＝TIA×PA+TIB×PB
In the above formula: TTI- -theoretical toxicological index of the mixture; toxicity index of TI A - -A agents; the percentage content of the P A -A medicament in the mixture is expressed as percentage (%); toxicity index of TI B - -B agent; the percentage of the P B -B medicament in the mixture is expressed as percentage (%).
In the above formula: ctc—co-toxicity coefficient; ati—actual measured virulence index of the mixture; tti—the theoretical toxicity index of the mixture.
5. Measurement results
See tables 1-3.
Table 13 toxicity determination results of single agent on Alternaria grunniens
As can be seen from table 1, the EC 50 for three single doses of asarone, polyoxin and natamycin for anthrax grus are respectively:
TABLE 2 determination of the Combined toxicity of asarone and polyoxin different mass proportions of the mixture to Alternaria gruca
As can be seen from the combination of the table 1 and the table 2, asarone, polyoxin and the mixture thereof have good inhibition effect on the flat-head anthracnose of the pepper anthracnose. The mass ratio of asarone to polyoxin is 1-30:8-1, the co-toxicity coefficient for inhibiting the colletotrichum gloeosporioides is more than 120. The asarone and the polyoxin of the invention show synergism under the mass ratio, and can improve the control effect on pepper anthracnose.
TABLE 3 determination of the Combined toxicity of asarone and natamycin blends of different mass ratios to Althaea crassifolia
It can be seen from the combination of Table 1 and Table 3 that asarone, natamycin and the mixture thereof have good inhibition effect on the flat-headed anthracnose of pepper anthracnose. The mass ratio of asarone to natamycin is 1-4:27-1, the co-toxicity coefficient for inhibiting the colletotrichum gloeosporioides is more than 120, and the mass ratio is 1:18. the asarone and the natamycin of the invention show synergism under the mass ratio, and can improve the control effect on pepper anthracnose.
In conclusion, the asarone compound formula with good inhibition effect on pepper anthracnose is screened on the basis of good inhibition activity of the existing asarone on plant pathogenic fungi. Indoor combined toxicity tests show that when asarone and polyoxin or natamycin are compounded according to a certain mass ratio, the co-toxicity coefficient of pathogenic bacteria of pepper anthracnose is above 120, and the synergistic effect is shown, so that the control effect on pepper anthracnose can be improved.
The foregoing descriptions of specific exemplary embodiments of the present invention are presented for purposes of illustration and description. It is not intended to limit the invention to the precise form disclosed, and obviously many modifications and variations are possible in light of the above teaching. The exemplary embodiments were chosen and described in order to explain the specific principles of the invention and its practical application to thereby enable one skilled in the art to make and utilize the invention in various exemplary embodiments and with various modifications as are suited to the particular use contemplated. It is intended that the scope of the invention be defined by the claims and their equivalents.
Claims (6)
1. The pesticide composition for preventing and treating pepper anthracnose is characterized in that the active ingredients of the pesticide composition are prepared from asarone and polyoxin or natamycin according to the proportion of 1-150:150-1 by mass ratio.
2. The pesticide composition as set forth in claim 1, wherein the mass ratio of asarone to polyoxin is 1-30:8-1.
3. The pesticide composition as set forth in claim 1, wherein the mass ratio of asarone to natamycin is 1-4:27-1.
4. A pesticide composition as set forth in claim 3, characterized in that the mass ratio of asarone to natamycin is 1:18.
5. Use of the pesticidal composition according to any one of claims 1 to 4 for controlling a pepper disease or for preparing a medicament for controlling a pepper disease.
6. The use according to claim 5, wherein the pepper disease is pepper anthracnose.
Priority Applications (1)
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