CN103170386A

CN103170386A - Pipette tips

Info

Publication number: CN103170386A
Application number: CN2012105599585A
Authority: CN
Inventors: J·贝泽
Original assignee: Eppendorf SE
Current assignee: Epedov Europe
Priority date: 2011-12-23
Filing date: 2012-12-21
Publication date: 2013-06-26
Anticipated expiration: 2032-12-21
Also published as: JP5775059B2; CN103170386B; EP2606977A1; JP2013136052A; EP2606977B1

Abstract

The tip (1) has a seat section provided next to an upper opening (6) at an inner periphery. The seat section includes a holding region with radial inward projecting part, axially extending ribs and a sealing region that is formed below the holding region. The sealing region is formed, so that the tip is held and sealed on a neck, during insertion of the tip with the seat section on the neck by fitting force. The ribs are plastically deformed, and an elastic deformation occurs on an outer side of the ribs in the seat section.

Description

Pipette tip

Technical field

The present invention relates to a kind of pipette tip.

Background technology

Together with pipette and other metering device, pipette tip is used for metering liquid.Hereinafter, pipette and metering device are generically and collectively referred to as " pipette device ".Pipette tip has elongate tubular body, and described main body has at the pipette opening of lower end with for the grafting opening on the annex that is plugged into the pipette device.Standardized cone shape annex with standard geometrical shapes is known, its as one man used by many manufacturers and for each pipette tip by the average diameter of conical attachment than and the cone angle ratio be feature.

The pipette device comprises gas discharge element, and described gas is discharged element and normally had axially movable therein cylindrical piston.Gas is discharged the access opening that element is connected to annex.Be pressed into by the annex with pipette in the upper shed of pipette tip, pipette tip is clamped to annex hermetically.

Move air column so that for being positioned at the pipette tip pumping liquid on annex or releasing liquid from it by means of gas discharge element.When air column moved away from pipette tip, a certain amount of liquid was drawn in pipette tip by under shed.By moving air column towards pipette tip, a certain amount of liquid is discharged to outside pipette tip by under shed.

The pipette device has pushing piece usually, and described pushing piece acts on the upper limb of pipette tip to push it open from annex.By like this, avoided the user must contact contaminated pipette tip in order to it is broken away from from annex.

The pipette device can be that the user can be with the hand-held pipette of a grasped and operation.The pipette device can be also measuring station or be incorporated in work station.In all cases, gas discharge element can be manually or by motor operated.The grafting of pipette tip or release also can manually or by motor be undertaken.

For fear of the pipette action of mistake, pipette tip must be fixed on annex hermetically.In addition, being used for pipette tip pegs graft and the power released must be not too high from annex.In conventional pipette tip, the contact area between pipette tip and conical attachment is similar to frustum of a cone.When the grafting pipette tip, pipette tip is flexibly expanded by annex at its circumference.Spring performance is uprushed, and makes to use high mating force.After pegging graft, corresponding high static friction acts between annex and pipette tip.Must overcome this static friction when releasing pipette tip.The user is subject to perplexing for the large active force of grafting and release pipette tip.As long as peg graft and release by motor driver, these must be correspondingly powerful and they have high energy consumption.

Document US6,197,259B1 has described a kind of less axial grafting and the release power annex that easily and securely is installed to pipette or the pipette tip of releasing from described standardized cone shape annex that can be by applying six pounds and three pounds (being respectively 26.7N and 13.3N).This pipette tip has tubulose pipette part, and described tubulose pipette partly has conical upper end, and the internal diameter that has in the top is greater than will the peg graft diameter of annex of pipette thereon of pipette tip.In addition, pipette tip has the annular seal zones of hollow central part and the junction between upper end and middle body.The internal diameter that annular seal zones has is less than value " x " and be designed such that it engages with the lower end of the band of annex, thereby expand towards outer radial ground when inserting annex, produce thus the impenetrable liquid sealing between the sealing area of the band of annex and pipette tip.In addition, pipette tip has the lateral stabilizer near place, the inboard of sealing area, and described lateral stabilizer engages with the outer surface of annex in order to stablize pipette tip on annex.These lateral stabilizers have isolated at least three contacts in a circumferential direction, and described contact extends from the interior face of pipette tip is inner.The size of the diametral distance of contact is determined to be and makes them easily engage and make the lower end of annex to slide and the described sidewall that do not expand along the sidewall of contact pipette tip disposed thereon with the free end of annex.When the side direction External Force Acting during in pipette tip, for example when liquid on wall during landing, contact is realized the lateral support of the pipette tip on annex, in order to prevent that pipette tip from moving to the side.When the lower end of the band of annex engaged the sealing area of pipette tip, pipette tip expanded at sealing area with in being close to its zone.At guiding pipette tip on annex and when stably and safely aiming at it on annex, the sidewall of the supporting contact of pipette tip is to internal strain and do not expand when contact, thus in insertion only small power put on annex.

Document US7,339,373B1 have described a kind of ergonomic designs pipette tip, and it can be plugged on the axle of pipette safely, peg graft simultaneously and release axial force used obviously to reduce.This ergonomics pipette tip is included in the thin expansion clearance of making in injection moulding, described thin expansion clearance is joined by thicker wall section and/or the second elastomeric material side, when introducing in the sealing area of pipette tip with convenient pipette axle according to flexural spring principle reduce the to expand necessary power of upper limb of pipette tip.And pipette tip has one or several projections of side within it, and described projection is intended to limit the pipette axle and enters insertion depth as the pipette tip of disabler.

It is most advanced and sophisticated that document EP1520624B1 has described a kind of plastic suction pipet with elongate tubular body, and described tubular body has at the opening of lower end and is used for being plugged into grafting opening on the fixed axis of pipette device in the upper end.Pipette tip has tie near upper end, described tie along closed curve around and have a different distance from the lower end at different circumferential positions.Have contact area in the inboard around tie, described contact area on Plane of rotation around.Other tie is from farthest the position around tie begins to extend to the upper end from the lower end always.Use is around the contact area on the inboard of tie, and pipette tip contacts with the annex sealing of pipette device.When releasing, the pushing piece of pipette device presses against the other tie of the upper end of pipette tip.By other tie, release power is delivered to from the lower end position around tie farthest.These positions around tie are extruded towards the lower end thus a little.With respect to the directed plane that meets at right angles of the tubular body with pipette tip, be squeezed into more flat a little around tie.Tie forms rigid region in addition, and described rigid region only is subject to than small deformation in the transmission of power.Due to directed across around tie and other tie, therefore can relatively easily be out of shape it by the power of introducing on the direction of other tie.Therefore, expand a little around tie, and correspondingly reduce respectively around the inboard of tie or the static friction between contact area and annex.Therefore, being used for making pipette tip to break away from necessary power from fixed axis reduces.And pipette tip is flexible larger in the zone around tie, makes for the power that it is plugged on annex even to reduce, and helps to be plugged on the fixed axis with different size.

In the pipette tip of above document, the change in size of pipette tip and annex is by the elastic expansion compensation of pipette tip.When the internal diameter of the pipette tip in the holder zone during well below standard size, perhaps when the external diameter of annex during considerably beyond standard size, will produce and be used for the more high-tensile strength of pegging graft and releasing.

Summary of the invention

Thus, the present invention is based on of task is to provide a kind of pipette tip, and the annex that has wherein reduced to be used for from the pipette device is released the necessary release power of pipette tip, and dimensional tolerance is less on the impact of releasing power.

This task is realized by the pipette tip of the feature with claim 1.Indicated in the dependent claims the advantageous embodiment of pipette tip.

pipette tip of the present invention has elongated little tube shape, and have the lower end be used for by fluid under shed and in the upper shed of upper end, wherein the holder zone is positioned on the inner periphery of described upper shed and is used for pipette tip is plugged into the standardized cone shape annex of pipette device, wherein said holder zone has the retaining zone that extends axially rib and the sealing area under described retaining zone of giving prominence to towards inner radial, and be configured to make and be plugged on described standardized cone shape annex to guarantee that described pipette tip keeps when described pipette tip by mating force with its holder zone and when being sealed on described standardized cone shape annex, described extending axially in the described holder zone that flank ground plastic deformation and strain occur in the described outside that extends axially rib.

When pipette tip of the present invention was plugged on the standardized cone shape annex of pipette device, the surface pressing in the zone of axial rib caused the part plastic deformation of axial rib.In addition, pipette tip is in the holder zone Elastic distortion of the outside of elastic rib.The part of mating force is by the plastic deformation consumption of rib, and another part of mating force is stored in strain.Therefore, pipette tip remains on annex under bias voltage.Rib laterally supports pipette tip, makes it not shake under the impact of side force.In order to release pipette tip, only must overcome the power that is stored by strain.When pipette tip is pushed out, needn't be as again storing the part of making every effort to overcome the plastic deformation that is assigned to axial rib that takes mating force.Release power must overcome the frictional force that exists due to the fexible bias pressure between pipette tip and annex.The dimensional tolerance of pipette tip and annex by the plastic deformation of rib compensation and on the impact of the strain in the holder zone than less with conventional pipette tip.By reducing like this dimensional tolerance to releasing the impact of power.

Therefore, only must expend little release power and release pipette tip of the present invention from annex.This has removed and has used the burden that has by the hand-held pipette of the pushing piece of thumb-actuated.The user can easily apply necessary mating force with forearm and hand.Similarly, pipette tip of the present invention has also been removed the burden of motor driving pushing piece.

Strain in the holder zone can occur in retaining zone in sealing area and/or between rib.Strain can be limited to the zone between sealing area or rib, and perhaps it can occur in zone in sealing area and between rib.Strain in the plastic deformation of rib and holder zone is by the geometry in holder zone and the material decision of pipette tip.The plastic deformation of rib is determined by the rib profile especially.The wall thickness of the pipette tip in the zone of rib is determined the size of the strain in this zone.Wall thickness in the zone of rib can be selected as the large pipette tip performance that makes rigidity, and the plastic deformation of rib only occurs.Elasticity in sealing area or plastic deformation can be especially determined by the wall thickness of the pipette tip in sealing area, and depend on the circumstances by in the sealing area of inside around the geometry of sealing protuberance.

For each size of pipette tip, standardized cone shape annex is limited by certain average diameter and certain cone angle.This sums up in following table:

The size of pipette tip (microlitre)	Average diameter (mm)	Cone angle (degree)
			0.1-10	2.95	4.77
0.1-20	2.95	4.77
			0.5-20	3.3	6.68
2-20	4.4	6.99
			20-30	4.4	6.99
50-1000	7.1	2.71
			50-1250	7.1	2.48
500-2500	9.3	3.78
			100-5000	12.4	4.00
1000-10000	14.3	4.35
			10-10000	14.3	4.35

The tolerance of the 0.05mm of+/-is applicable to the diameter standard, and+/-0.3 ° be applicable to the angle gauge model.

According to an embodiment, the described rib that extends axially distributes on circumference equably.By helping like this homogeneous deformation and the support of the pipette tip in the holder zone.

According to another embodiment, described pipette tip has 3 to 12 ribs, preferred 5 to 7 ribs, and more preferably 6 ribs.Support uniformly by such acquisition.The described quantity that extends axially rib has the plastically deformable rib geometry that can easily produce.

According to another embodiment, the described size that extends axially rib is determined to be and makes: be plugged on described standardized cone shape annex to guarantee that described pipette tip keeps by mating force when described pipette tip and when being sealed on described standardized cone shape annex, for the occupied position of described standardized cone shape annex, described size and described standardized cone shape annex overlapping is 0.1mm at least, preferably 0.2mm and/or maximum 0.5mm at least, preferred maximum 0.3mm.The overlapping part plastic deformation that helps rib of this size.

According to another embodiment, described pipette tip in inside and then described upper shed place have towards inner radial outstanding extend axially in addition rib, the described rib that extends axially does not in addition have the described rib that extends axially to extend far towards inner radial, and the described rib that extends axially in addition is arranged in two described extending axially between rib.Rib can be used for pipette tip additionally is directed to annex in addition, and wherein when pipette tip was plugged on annex, rib can not stand distortion in addition, or only is subject to the distortion less than rib.According to preferred embodiment, the size of rib is determined to be and makes in addition: be plugged on described standardized cone shape annex to guarantee that described pipette tip keeps by mating force when described pipette tip and when being sealed on described standardized cone shape annex, not distortion of rib in addition.

According to another embodiment, describedly extend axially rib and/or the described rib that extends axially in addition has at the profile of convergent on the direction of inside radially.By helping like this axial rib plastic deformation in the inner of profile.According to another embodiment, describedly extend axially rib and/or the described rib that extends axially in addition has the trapezoidal or parabolical profile of triangle.

According to preferred embodiment, described pipette tip has the groove on externally circumference, described groove and describedly extend axially rib and/or described to extend axially in addition rib overlapping.Groove for example is used for making pipette tip to have rigidity in the rib zone, perhaps is used for characterizing pipette tip.In the situation that they are used for characterizing pipette tip, the layout of groove arrangement on rib and/or other rib helps the identifiability of the groove on transparent pipette tip.Otherwise rib and/or other rib can be considered to groove.

According to another embodiment, described pipette tip is in arranging the described zone that extends axially rib and/or expand towards described upper shed in described sealing area conically.The insertion of the annex by helping like this pipette device.In principle, if rib is fitted on standardized cone shape annex mutually by plastic deformation, and have in sealing area around the sealing protuberance, sealing area abuts against described sealing protuberance and is bearing on annex, and described zone can have cylindrical shape rather than cone shape.

According to another embodiment, described sealing area have towards inside outstanding and within it on section's circumference around the sealing protuberance.The sealing protuberance for example is used for sealing bead or sealing lip limit.

According to another embodiment, described pipette tip is arranging that the described wall thickness that has in extending axially the zone of rib is greater than the wall thickness in described sealing area.Pipette tip is harder in the zone of axial rib, helps thus the plastic deformation of rib.

According to another embodiment, described pipette tip has for restriction and is plugged into restraint device on described standardized cone shape annex, when obtaining mating force guaranteeing described pipette tip and keep and to be sealed on described standardized cone shape annex and during the described part plastic deformation that extends axially rib, described restraint device stops and is plugged on described standardized cone shape annex.Plastic deformation by such axial rib can be limited to suitable degree.The restraint device that is used for restriction can be formed by the butting section, and described butting section is by forming from one or several outstanding projections of the inner periphery of pipette tip towards inside.

According to another embodiment, described restraint device of pegging graft for restriction is the brake area of expanding towards described upper shed under described sealing area conically.When execution was plugged on standardized cone shape annex, the lower edge of annex clashed into brake area, and the introducing campaign of annex is stoped by the cone shape of brake area gradually.By like this, to compare with the conventional butting section of the motion of pegging graft with cut, burden for users is less.

Described pipette tip is preferably by the plastic material manufacturing.More preferably, described pipette tip is by polyolefin, preferably by polypropylene or polyethylene manufacturing.According to another embodiment, pipette tip is light-permeable, preferably clear.More preferably, pipette tip is made by injection mo(u)lding.Internal diameter and/or the external diameter of pipette tip preferably increase towards upper shed under shed.

Description of drawings

Hereinafter, explain in more detail the present invention by the accompanying drawing of embodiments of the invention.In the drawings:

Fig. 1 a-e in side view (Fig. 1 a), in the longitudinal section (Fig. 1 b), in looking up enlarged drawing (Fig. 1 c), in overlooking enlarged drawing (Fig. 1 d) and in sectional perspective view (Fig. 1 e) shown have axial rib and around the sealing protuberance pipette tip;

Fig. 2 in side view shows pipette tip and the annex when the pipette tip of Fig. 1 is pegged graft;

Fig. 3 has shown the annex in the holder zone of the pipette tip that is inserted into Fig. 1 in the part longitudinal section;

Fig. 4 shown the grafting of pipette tip of the present invention and release power (Fig. 4 a) with the comparison of conventional pipette tip (Fig. 4 b);

Fig. 5 has shown average diameter and the cone angle of the standardized cone shape annex of the pipette tip that is used for the grafting different size in total view.

The specific embodiment

In this application, the deictic word "up" and "down" refers to the orientation of the pipette tip in pipette, and wherein pipette tip is directed vertically, and under shed in the bottom side and upper shed in the top side.

In the following description of different embodiment, same characteristic features is represented by same reference numerals.

According to Fig. 1, pipette tip 1 has elongate tubular body 2, and this main body has in the lower end 3 under

shed

4 and 5 the grafting opening 6 in the upper end.Under shed 4 is less than upper shed 6.

46 increases towards the upper end from the lower end substantially of the internal diameter of tubular body 2 and/or external diameter.In example, tubular body 2 has the conical forward part 7 in the bottom side, the middle body 8 that the taper shape on it is less and the head part on middle body 89.Pipette tip 1 of the present invention also can be with other mode, particularly in part or continuously 6 expansions under shed 4 towards upper shed.

Head part 9 has the introducing zone 10 with enlarged diameter at next-door neighbour upper shed 6 places, described enlarged area is only extended on the short part of head part 9.Retaining zone 11 is connected to be introduced under zone 10, and from the beginning the inner periphery of part is outstanding towards inside for its middle rib 12,13.They are far away to extend axially rib 12 and the outstanding nearer other rib 13 that extends axially towards inside towards inner radial is outstanding.In Fig. 1 d and 1e, can be clear that rib 12 is outstanding more farther from the inner periphery of pipette tip 1 than other rib 13.An other rib 13 always is arranged between two ribs 12.

Rib 12 and other rib 13 all have in the top side near the

short chamfering

14,15 of introducing zone 10.

Head part 9 side thereon has the cylindrical region 16 outstanding towards the outside.Cylindrical region 16 is by outer step 17 restrictions in its bottom side.Head zone has interior step 18 in inside.Rib 12 and other rib 13 extend through interior step 18.They are in the place, bottom side ends at sealing area 19.There, be weld shape from the beginning the circumference of part 9 is outstanding in inside around sealing protuberance 20.Rib 12 and other rib 13 extend to sealing protuberance 20 always.Rib 13 has the step position 21 in the zone of interior step 18 in addition.

Rib 12 and in addition rib 13 have

parabolic outlines

22,23 in zone on interior step 18.This profile has the evener 24,25 of giving prominence to towards inside farthest in this zone.At first,

parabolic outlines

22,23 is expanded the

position

26,27 up to Breadth Maximum on direction from the top down, and it narrows down again therefrom, until interior step 18.

In addition, head zone has water-drop-shaped and the outside outstanding groove 28 on the exterior periphery of cylindrical region 16.On interior step 18, the imbricate of the edge of the edge of groove 28 and rib 12 and other rib 13.

Under sealing protuberance 20, conical brake area 29 connects sealing area 19, and downward convergent and upwards expansion respectively.

In

rib

12,13 zone, head part 9 is on interior step 18 and under interior step 18 and slightly be conical in inside in sealing area 19.In brake area 29, it is larger taper.

The zone of rib 13, sealing area 19 and brake area 29 will be generically and collectively referred to as " holder zone " 30 in addition.

Under brake area 29, middle body 8 restriction main bodys 2.

According to Fig. 2, hand-held pipette 31 has the shell 32 that is handle shapes, described shell comprises for the discharge element 33 of discharging air, and arranges that on the discharge element being used for actuating discharges the actuation element 34 of element 33 and the pushing piece 35 with connected other actuation element 36.In the bottom side, standardized cone shape annex 37 is arranged on the bottom side of shell 32.

Pipette tip 1 can be plugged on annex 37.

According to Fig. 3, when pipette tip 1 in its holder zone 30 when being plugged on standardized cone shape annex 37, partly plastic deformation of rib 12.Like this is exactly in the overlapping region of the annex 37 in Fig. 3 and profile rib 12.The wall of pipette tip 1 is in rib 12 and plastic deformation a little between rib 13 in addition.In the zone of sealing protuberance 20, holder zone 30 is distortion flexibly, reason be wall with respect to shown in the position a little towards outside deflection, overlap each other at the profile of described position sealing protuberance 20 and annex 37.

Brake area 29 plastic deformation in the zone of the lower end 38 of annex 37 (left side in Fig. 3).Like this profile overlapping of lower annex end 38 and brake area 29 is exactly.

According to Fig. 4 a, the part of mating force need to be used for the plastic deformation of pipette tip.The other part of mating force is assigned to the elastic part of the distortion of pipette tip 1.Therefore, the part of mating force irreversibly consumes to be used for the plastic deformation of pipette tip 1.Eject the required release power of pipette tip 1 from annex and only must overcome the part of the mating force of the strain of distributing to pipette tip 1.

Therefore, than with only by the conventional pipette tip 1 of mating force strain, use in the situation of pipette tip 1 of the present invention, release power is less.In conventional pipette tip, mating force and release power are identical.This shows in Fig. 4 b.

Fig. 5 has shown at corresponding average diameter and cone angle with the standardized cone shape annex 37 on the related pipette tip 1 of certain syringe size.Average diameter represents with millimeter and the cone angle expenditure represents.As the degree of pipette tip size, the respective range of the packing volume that pipette tip 1 can be filled represents with microlitre and milliliter respectively.The average diameter of annex 37 is relevant with the diameter between the upper end to the lower end of annex 37.Must not force to process the lower end that just in time is in annex and the diameter of the centre between the upper end.Yet average diameter also can just in time be in the lower end of annex and the centre between the upper end.

In addition, in Fig. 5 the tolerance that represents of expression average diameter (+/-0.05mm) and the tolerance (+/-0.3 °) that represents of angle.Annex 37 produces with corresponding tolerance.

The list of Reference numeral

1 pipette tip

2 tubular bodies

3 lower ends

4 under sheds

5 upper ends

6 upper sheds

7 conical forward part

8 conical middle bodies

9 head part

10 introduce the zone

11 retaining zones

12 extend axially rib

The 13 other ribs that extend axially

14 short chamferings

15 other short chamferings

16 cylindrical regions

17 outer steps

18 interior steps

19 sealing areas

20 sealing protuberances

21 step positions

22 parabolic outlines

23 other parabolic outlines

24 eveners

25 eveners

The position of 26 Breadth Maximums

The position of 27 Breadth Maximums

28 water-drop-shaped grooves

29 conical brake area

30 holders are regional

31 hand-held pipette

32 shells

33 discharge element

34 actuation elements

35 pushing pieces

36 other actuation elements

37 conical attachments

The lower end of 38 annexes

Claims

1. pipette tip, described pipette tip has elongated little tube shape, and have in the lower end (3) be used under shed (3) that liquid passes through and in the upper shed (6) of upper end, wherein holder zone (30) are positioned on the inner periphery that is adjacent to described upper shed (6) and are used for described pipette tip is plugged into the standardized cone shape annex (37) of pipette device, wherein said holder zone (30) has with the retaining zone that extends axially rib (12) (11) of giving prominence to towards inner radial and the sealing area (19) under described retaining zone (11), and be configured to make when by mating force, described pipette tip (1) being plugged into described standardized cone shape annex (37) by described holder zone (30) upper to guarantee that described pipette tip (1) keeps and was sealed in described standardized cone shape annex (37) when upper, describedly extend axially partly plastic deformation of rib (12), and generation strain in the described holder zone (30) of the described outside that extends axially rib (12).

2. pipette tip according to claim 1, the wherein said rib (12) that extends axially distributes on circumference equably.

3. pipette tip according to claim 1 and 2, described pipette tip has 3 to 12 ribs (12), preferred 5 to 7 ribs, and more preferably 6 ribs.

4. the described pipette tip of any one according to claim 1 to 3, the wherein said size that extends axially rib (12) is determined to be and makes: be plugged into described standardized cone shape annex (37) at described pipette tip (1) by mating force upper to guarantee that described pipette tip (1) keeps and was sealed in described standardized cone shape annex (37) when upper, for the occupied position of described standardized cone shape annex (37), the size of described size and described standardized cone shape annex (37) is overlapping is 0.1mm at least, preferably 0.2mm and/or maximum 0.5mm at least, preferred maximum 0.3mm.

5. the described pipette tip of any one according to claim 1 to 5, wherein said pipette tip be close to described upper shed (6) locate to have inside and towards inner radial outstanding extend axially in addition rib (13), the described rib that extends axially does not in addition have the described rib that extends axially to extend far towards inner radial, and the described rib that extends axially in addition is arranged in two described extending axially between rib.

6. pipette tip according to claim 5, the wherein said size that extends axially in addition rib (13) is determined to be and makes: to be plugged into described standardized cone shape annex (37) by mating force upper guaranteeing that described pipette tip (1) keeps and was sealed in described standardized cone shape annex (37) when upper when described pipette tip (1), describedly extends axially in addition not distortion of rib.

7. the described pipette tip of any one according to claim 1 to 6 wherein saidly extends axially rib (12) and/or the described rib (13) that extends axially in addition has at the profile towards the convergent in the radial direction of inside.

8. pipette tip according to claim 7 wherein saidly extends axially rib (12) and/or the described rib (13) that extends axially in addition has triangle, trapezoidal or parabolical profile.

9. the described pipette tip of any one according to claim 1 to 8, described pipette tip have the groove (28) on externally circumference, described groove and describedly extend axially rib (12) and/or described to extend axially in addition rib (13) overlapping.

10. the described pipette tip of any one according to claim 1 to 9, described pipette tip is in arranging the described zone that extends axially rib (12) and/or expand towards described upper shed in described sealing area (19) conically.

11. the described pipette tip of any one according to claim 1 to 10, described pipette tip have towards inside outstanding and on the circumference of described sealing area (19) around sealing protuberance (20).

12. the described pipette tip of any one according to claim 1 to 11, the wall thickness of described pipette tip in described retaining zone (11) is greater than the wall thickness in described sealing area (19).

13. the described pipette tip of any one according to claim 1 to 12, described pipette tip has for restriction and is plugged into restraint device on described standardized cone shape annex (37), when having obtained mating force when guaranteeing described pipette tip (1) and keep and being sealed in the upper and described part plastic deformation that extends axially rib (12) of described standardized cone shape annex (37), described restraint device stops and is plugged on described standardized cone shape annex (37).

14. pipette tip according to claim 13, the described restraint device (29) that wherein is used for the restriction grafting is the brake area (29) of expanding towards described upper shed under described sealing area (19) conically.

15. the described pipette tip of any one according to claim 1 to 14, described pipette tip is made by plastic material, preferably by polyolefin, more preferably made by polypropylene or polyethylene.