CN109845736A - Bactericidal composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion - Google Patents

Abstract

The present invention provides a kind of microbicide compositions, the composition includes two kinds of effective constituents As and B, and active component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is Physcion, and the weight ratio between two components is 1:10~80:1.The present invention also provides the Preparation method and uses of the composition.Test result shows that above-mentioned bactericidal composition synergy provided by the invention is obvious, it is often more important that amount of application is reduced, and use cost reduces.The bactericidal composition can effectively prevent certain specific fungal diseases of crop.By compounding the fungicide of different role mechanism and the mode of action, the amount of application of each single dose is effectively reduced, there is good effect for expand fungicidal spectrum and delay fungi, fungus resistant and improve control efficiency etc..

Description

Bactericidal composition containing toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion

Technical field

The invention belongs to agriculture plant protection fields, more particularly to a kind of with the bactericidal composition for improving performance, tool Say to body it is to be related to a kind of bactericidal composition comprising toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion.

Background technique

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein (plant activator protein) is through Alternaria tenuissima fermentation, extracts Biologically active, single, the stable protein of one kind.The protein is made of 207 amino acid.ToVerticilliumdahliaActivitie Activitie S of Relative Activator protein be from a kind of biological activity protein of the fermented extraction of natural microbial, after touching plant organ surface, It can be in conjunction with the receptor protein on plant cell membrane.Cause a series of related enzyme activities increases and gene expression amount in plant Enhancing, a series of intracorporal metabolic regulation of excitation plant promote the growth of plant roots cauline leafs, improve chlorophyll content, improve and make Produce amount.

Physcion (Physcion) is highly active vegetable source bactericide, using natural plants rheum officinale as raw material, through meticulous Its active constituent is extracted, processing develops, and has good control efficiency to powdery mildew, downy mildew, gray mold, anthracnose etc.. It is environmentally friendly to people and animals' low toxicity, particularly suitable for green and organic vegetable production.Physcion is protective fungicide, It induces crop to generate defense reaction, inhibits pathogen spore germination, the growth of mycelia, the formation of haustorium, so that crop is from disease The infringement of opportunistic pathogen, achievees the effect that diseases prevention.

Actual pesticide experience have shown that, repeat and single-minded apply a kind of reactive compound to prevent and treat disinfect pathogen very It will lead to the fast selective of bacterial strain in more situations, it is usually used at present different for the risk for reducing resistant strain selectivity The mixture of reactive compound prevents and treats harmful crop germ.Group is carried out by the reactive compound that will there is different role mechanism Close, can delaying drug resistance generate, reduce amount of application, reduce control cost.

Summary of the invention

The purpose of the present invention is being directed to fungicide resistance and pedo relict problem in practical applications, filters out difference and kill The fungicide of bacterium principle is compounded, and a kind of new microbicide compositions are obtained, to improve bactericidal agent for preventing and treating effect, delaying drug resistance It generates, reduces amount of application, reduce control cost.

It is a further object to provide prevent and treat comprising two kinds of effective constituents As and B bactericidal composition in agriculture field Application in corps diseases.

The purpose of the present invention can be achieved by the following measures:

A kind of microbicide compositions with synergistic effect, the composition includes two kinds of active components of A and B, wherein activity Component A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is Physcion.

Inventor is found by experiment that composition of the invention is prevented and treated for preventing and treating crops bacterium or fungal disease Synergy is obvious, it is often more important that amount of application is reduced, and reduces use cost.Compound structure type containing component A and component B is not Together, mechanism of action is different, and the two compounding can expand fungicidal spectrum, and can delay the production of pathogen resistance to a certain extent Raw and development speed, and no interactions resistance between component A and component B.

The weight ratio between two components in microbicide compositions of the present invention is 1:10~80:1, in a kind of preferred embodiment In, the weight ratio of active component A and active component B are 1:5~70:1.

In some embodiments, two kinds of active component As and B can be selected 80:1,79:1,78:1,77:1,76:1, 75:1、74:1、73:1、72:1、71:1、70:1、69:1、68:1、67:1、66:1、65:1、64:1、63:1、62:1、61:1、 60:1、59:1、58:1、57:1、56:1、55:1、54:1、53:1、52:1、51:1、50:1、49:1、48:1、47:1、46:1、 45:1、44:1、43:1、42:1、41:1、40:1、39:1、38:1、37:1、36:1、35:1、34:1、33:1、32:1、31:1、 30:1、29:1、28:1、27:1、26:1、25:1、24:1、23:1、22:1、21:1、20:1、19:1、18:1、17:1、16:1、 15:1、14:1、13:1、12:1、11:1、10:1、9:1、8:1、7:1、6:1、5:1、4:1、3:1、2:1、1:1、1:2、1:3、1: 4,1:5,1:6,1:7,1:8,1:9,1:10 etc. are more specifically matched, and composed range can also be matched at any of the above two It is inside selected, proportion of the invention can be understood as the ratio or mass ratio or molar ratio of weight and volume.

In the present invention, toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion be can be understood as comprising respective compound itself The structural derivative product of all kinds of other forms is only simple base group modification, such as all kinds of salt compounds, ester type compound.

The dosage form allowed on pesticide can be made by active constituent and insecticides adjuvant or auxiliary material in composition of the invention.Into one Step, the composition is by the active component of 0.5~30% parts by weight and the insecticides adjuvant or auxiliary material system of 99.5~70% parts by weight At the dosage form allowed on pesticide.

The present invention provides the bactericidal compositions comprising component A and component B in terms of agriculture field prevents and treats corps diseases Purposes, the especially purposes in terms of the fungi or bacterial disease for preventing and treating certain crops.

Above-mentioned composition specifically may include insecticides adjuvant or auxiliary material, such as carrier, solvent, dispersing agent, wetting agent, gluing One or more of agent, thickener, adhesive, surfactant or fertilizer etc..It can be mixed during application common Auxiliary agent.

Suitable auxiliary agent or auxiliary material can be solid or liquid, they are usually common substance during formulation, Such as natural or regenerated minerals, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive.

The method of administration of the present composition includes the aerial part that composition of the invention is used for plant growth, especially It is leaf portion or blade face.It can choose seed soaking or be applied to controlling object surface.The frequency and amount of application of application depend on pathogen Biology and weather life condition.The growth place of plant, such as rice field can be soaked with the liquid preparation of composition, or Composition is applied in soil by person in solid form, and such as in granular form (soil application), composition can be by soil through planting Object root enters in plant (systemic action).

Composition of the invention can be prepared into various dosage forms pesticide-acceptable, including but not limited to missible oil, suspension Agent, wettable powder, water dispersible granules, pulvis, granula, aqua, aqueous emulsion, microemulsion, poison bait, mother liquor, female powder etc., in one kind In preferred embodiment, dosage form of the invention uses aqua, suspending agent, wettable powder, water dispersible granules or microemulsion.According to these The property and application composition purpose to be achieved and ambient conditions of composition, can choose composition with by spraying, more Mist dusts, broadcasts sowing or sprinkles the method application poured or the like.

Composition of the invention can be prepared into various dosage forms by known method, it can be by effective component and auxiliary agent, such as Solvent, solid carrier can uniformly mix together with surfactant when needing, grind, be prepared into required dosage form.

Above-mentioned solvent can be selected from aromatic hydrocarbon, preferably contain 8-12 carbon atom, such as xylene mixture or substituted benzene, phthalein Esters of gallic acid, such as two octanoic acid of Dibutyl phthalate or phthalandione, fat hydrocarbon, such as thiacyclohexane or paraffin, the ether of pure and mild ethylene glycol and they And ester, such as ethyl alcohol, ethylene glycol, ethylene glycol monomethyl；Ketone, such as cyclohexanone, highly polar solvent, such as N- methyl -2- pyrrolidines Ketone, dimethyl sulfoxide or dimethylformamide and vegetable oil or vegetable oil, such as soybean oil.

Above-mentioned solid carrier, such as usually natural mineral fillers, such as talcum, kaolinite for pulvis and dispersible agent Soil, montmorillonite or atlapulgite.In order to manage the physical property of composition, polymolecularity silicic acid or polymolecularity can also be added Adsorpting polymerization object carrier, such as granulated adsorptive carriers or non-adsorbed carrier, suitable granulated adsorptive carriers are porous types, it is such as floating Stone, bentonite or bentonite；Suitable non-adsorbed carrier such as calcite or sand.Further, it is possible to use a large amount of inorganic in nature or having The prefabricated material to granulate of machine property is as carrier, especially dolomite.

The chemical property of effective component in composition according to the present invention, suitable surfactant are sulfomethylated lignin Acid, naphthalene sulfonic acids, phenolsulfonic acid, alkali salt or amine salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, fat Alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, there are also the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, naphthalene or The condensation product of naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, Alkylaryl polyglycol ether, tributyl benzene polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, Ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensation product, ethoxylation polyoxypropylene, laurel acid polyethylene glycol Ether acetal, sorbitol ester, lignin sulfite waste liquor and methylcellulose.

When preparing liquid dosage form, active component A first can be dissolved in alkaline matter, form toVerticilliumdahliaActivitie Activitie S of Relative activator protein Metal salt, suitable alkaline matter include: alkali carbonate, alkali metal hydroxide (such as sodium hydroxide, potassium hydroxide), alkali Metal alkoxide carbonate, alkali metal alcoholates or magnesium methoxide.

Two kinds of effective components show as synergistic effect in composition of the invention, and the activity of the composition is than using individualized The independent activity of the expected summation of activity and single compound of closing object is more significant.Synergistic effect shows as allowing amount of application Reduction, broader antifungal control spectrum, quick, more longlasting control efficiency, by a small number of applying only once or several times more Good control plant pest fungi and broaden the possible administration interval time.These characteristics are plant epiphyte control practices It especially needs in the process.

In terms of microbicide compositions of the invention can be applied to agriculture field prevention and treatment corps diseases, targeted specific disease Disease includes but is not limited to that paddy bacterial angular leaf spot, bacterial blight of rice, rice sheath blight disease, cucumber downy mildew, cucumber are bacillary The bacillary scab of angular leaf spot, watermelon blight, downy mildew of garpe, rice green smut, pepper anthracnose, bitter rot or anthracnose of grape, citrus Disease, cucumber anthracnose, celery septoria disease, lotus rhizome damping-off, powdery mildew of strawberry, yellow stunt of rice, downy mildew of lettuce, lettuce corruption Disease, celery gray mold, downy mildew, ginger leaf blight etc..

The other features of bactericidal composition of the invention shown are mainly shown as: 1, composition mixture of the invention tool There is apparent synergistic effect；2, since two single dose chemical structures of this composition are widely different, the mechanism of action is entirely different, no There are cross resistances, can delay two single doses that generated resistance problem is used alone；3, composition of the invention to crop safety, Preventive effect is good.Tests prove that microbicide compositions chemical property of the present invention is stablized, synergy is significant, shows to controlling object bright Aobvious synergy and complementation.

Specific embodiment

In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit The fixed present invention, any modifications, equivalent replacements and improvementsmade within the spirit and principles of the invention, etc. should be included in Within protection scope of the present invention.

Percentage is weight percentage in all formulas of following embodiment.The processing work of the various preparations of the present composition Skill is the prior art, can be varied according to different situations.

One, dosage form prepares embodiment

(1) processing and embodiment of wettable powder

Active components A and B activity ingredient and various auxiliary agents and filler etc. are sufficiently mixed in proportion, are made after being dissolved with water Aqua.

Embodiment 1:9.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion aqua

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 9%, Physcion 0.1%, alkyl polyglycoside 4%, N-Methyl pyrrolidone 3%, deionized water complements to 100%.

Embodiment 2:8.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion aqua

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 8%, Physcion 0.1%, polyacrylate 3%, Isopropanediol 0.9%, first Base tertbutyl ether 11%, deionized water complements to 100%.

Embodiment 3:7.2% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion aqua

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 6%, Physcion 1.2%, alkyl glycosides 7%, propylene glycol 1%, organosilicon 1%, deionized water complements to 100%.

Embodiment 4:2.2% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion aqua

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.2%, Physcion 2%, N-Methyl pyrrolidone 3%, alkylsulfonate 2%, isopropanol 2%, deionized water complements to 100%.

Embodiment 5:6.2% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion aqua

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 6%, Physcion 0.2%, propylene glycol 2%, N-Methyl pyrrolidone 4%, benzene Sodium formate 2%, organosilicon 2%, deionized water complements to 100%.

(2) processing and embodiment of suspending agent

By active constituent activity grouping A and active component B, with each components such as dispersing agent, wetting agent, thickener and water by matching The ratio of side is uniformly mixed, and after sand milling and/or high speed shear, obtains semi-finished product, and water supplement, which is uniformly mixed to filter, after analysis is Obtain finished product.

Embodiment 6:7.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion suspending agent

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 7%, Physcion 0.1%, white carbon black 2%, fatty alcohol polyoxyethylene ether phosphoric acid Ester 5%, Isopropanediol 1%, water complements to 100%.

Embodiment 7:6.1% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion suspending agent

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 6%, Physcion 0.1%, methyl naphthalene sulfonate formaldehyde condensate 2% are wooden Plain sodium sulfonate 4%, glycerine 5%, sodium benzoate 3%, water complements to 100%.

Embodiment 8:5% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion suspending agent

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 2.5%, Physcion 2.5%, bentonite 4%, sodium benzoate 4%, ethylene glycol 5%, xanthan gum 2%, water complements to 100%.

Embodiment 9:6.3% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion suspending agent

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 6%, Physcion 0.3%, methyl naphthalene sulfonate formaldehyde condensate 6%, the third two Alcohol 4%, sodium lignin sulfonate 4%, bentonite 3%, water complements to 100%.

(3) processing and embodiment of water dispersible granules

By active constituent activity grouping A and active component B, it is mixed according to the formula with auxiliary agent and filler uniformly, through gas Stream is ground into wettable powder, adds a certain amount of water mixing extrusion granulation, and water dispersible granules are made after dry screening and produce Product.

Embodiment 10:6.12% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion water dispersible granules

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 6%, Physcion 0.12%, precipitated calcium carbonate 2%, potassium dodecanesulfonate 4%, ammonium sulfate 3%, alkyl naphthalene sulfonic acid potassium 3%, bentonite complements to 100%.

Embodiment 11:0.6% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion water dispersible granules

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 0.1%, Physcion 0.5%, lauryl sodium sulfate 3%, lignin sulfonic acid Sodium 5%, methyl naphthalene sulfonate formaldehyde condensate 2%, white carbon black complements to 100%.

Embodiment 12:5.5% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion water dispersible granules

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 5%, Physcion 0.5%, dodecyl sodium sulfate 3%, methyl naphthalene sulfonic acid sodium Formaldehyde condensation products 3%, ammonium sulfate 5%, diatomite complements to 100%.

Embodiment 13:5.125% toVerticilliumdahliaActivitie Activitie S of Relative activator protein Physcion water dispersible granules

ToVerticilliumdahliaActivitie Activitie S of Relative activator protein 5%, Physcion 0.125%, lauryl sodium sulfate 3%, methyl naphthalene sulfonic acid Sodium formaldehyde condensation product 3%, ammonium sulfate 3%, precipitated calcium carbonate complements to 100%.

Two, drug effect verification test

(1) Assays Example

The incidence that whole strain blade is investigated according to test grade scale, calculates disease index and control efficiency.

Control efficiency is converted into probability value (y), liquor strength (μ g/ml) is converted into logarithm (x), with least square method Concentration EC50 in calculating virulence equation and inhibiting, the toxicity index and co-toxicity coefficient (CTC) of medicament are calculated according to Sun Yunpei's Method.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

Percentage composition+B medicament toxicity index * the mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture The percentage composition of middle B

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)] * 100

CTC≤80, composition show as antagonism, 80 < CTC < 120, and composition shows as summation action, and CTC >= 120, composition shows as synergistic effect.

1, toVerticilliumdahliaActivitie Activitie S of Relative activator protein is compounded with Physcion tests downy mildew of lettuce toxicity test

1. toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table is compounded with Physcion to lettuce rot disease toxicity test interpretation of result

As a result (table 1) shows toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion in 1:10~80:1, to lettuce corruption The control efficiency of disease significantly improves, and illustrates within this range, and the two compounding has significant synergistic effect to the prevention and treatment of lettuce rot disease.

(2) field efficacy verification test

Test method: it at the initial stage of a disease, carries out for the first time by spraying, carrying out being administered for second after 7 days immediately, each processing 4 A cell, each 20 square meter of cell.11 days investigation statistics incidences, 5 points of each cell are random before medicine and after second of medicine Sampling, 5 plants of crops of every investigation, the lesion area for investigating every blade in whole strain account for percentage and the classification of blade area, calculate Disease index and control efficiency.

It is expected that preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)

Grade scale:

0 grade: disease-free spot；

1 grade: leaf spot lesion is less than 5, and length is less than 1cm；

3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm；

5 grades: leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-25% of leaf area；

7 grades: leaf spot lesion 26 or more, scab is linked to be piece, and lesion area accounts for the 26-50% of leaf area；

9 grades: scab is linked to be piece, lesion area account for 50% of leaf area or more or full leaf it is withered.

1, toVerticilliumdahliaActivitie Activitie S of Relative activator protein compounds field efficacy experiment with Physcion

2 toVerticilliumdahliaActivitie Activitie S of Relative activator protein of table and Physcion mixture are to ginger leaf blight control efficiency

Measurement result (table 2) shows that toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion mixture prevent ginger leaf blight Effect significantly improves, and in 1:10~80:1, preventive effect collaboration occurs and increases especially toVerticilliumdahliaActivitie Activitie S of Relative activator protein and Physcion Effect effect illustrates that the two compounding has significant synergistic effect to ginger leaf blight.

Claims (7)

1. a kind of microbicide compositions, which is characterized in that the composition includes two kinds of active components of A and B, wherein active group Dividing A is toVerticilliumdahliaActivitie Activitie S of Relative activator protein, and active component B is Physcion, and the weight ratio between two components is 1:10~80:1.

2. microbicide compositions according to claim 1, which is characterized in that the weight ratio of active component A and active component B For 1:5~70:1.

3. microbicide compositions according to claim 1 or 2, which is characterized in that the composition is by active constituent and pesticide The dosage form allowed on pesticide is made in auxiliary agent or auxiliary material.

4. microbicide compositions according to claim 3, which is characterized in that the composition is by 0.5~30% parts by weight The dosage form allowed on pesticide is made in the insecticides adjuvant or auxiliary material of active component and 99.5~70% parts by weight.

5. microbicide compositions according to claim 3 or 4, which is characterized in that the dosage form be aqua, suspending agent, Wettable powder, water dispersible granules or microemulsion.

6. microbicide compositions according to claim 3 or 4, which is characterized in that the insecticides adjuvant or auxiliary material are selected from and carry One or more of body, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive, surfactant or fertilizer.

7. microbicide compositions described in any one of claim 1~6 are in terms of agriculture field prevents and treats corps diseases Purposes.