CN109526958A - Microbicide compositions containing rheum emodin and metconazole - Google Patents

Abstract

The present invention provides a kind of microbicide compositions, the composition includes two kinds of effective constituents As and B, and active component A is one of Physcion or aloe-emodin, and active component B is metconazole, and the weight ratio between two components is 10~1:1~120.The present invention also provides the Preparation method and uses of the composition.Test result shows that above-mentioned bactericidal composition synergy provided by the invention is obvious, it is often more important that amount of application is reduced, and use cost reduces.The bactericidal composition can effectively prevent certain specific bacteriums or the fungal disease of crop.By compounding the fungicide of different role mechanism and the mode of action, there is good effect for expand fungicidal spectrum and delay germ resistance and improve control efficiency etc..

Description

Microbicide compositions containing rheum emodin and metconazole

Technical field

The invention belongs to agriculture plant protection field, more particularly to a kind of with the microbicide compositions for improving performance, Concretely relate to a kind of microbicide compositions comprising emodin derivates class and metconazole.

Background technique

Physcion (Physcion) and aloe-emodin (Aloeemodin) belong to anthraquinone analog compound.In medicine These compounds of field are widely used, if immunoregulation acts on, scavenging activated oxygen effect, and antibacterial, anti-inflammatory, antiviral work With, diuresis, the protective effect to cerebrum ischemia damage, cardiovascu- lar effects, antitumor action.In recent years, these anthracenes Quinones is just gradually applied to agriculture field, for preventing and treating fungi or bacterial disease.Such compound is from medium-height grass A kind of highly active vegetable source bactericide extracted in the root of medicine plant rheum officinale, stem belongs to anthraquinone analog compound, to veterinary antibiotics, paddy The multiple diseases such as powdery mildew, downy mildew, gray mold, the anthracnose of object etc. have good control efficiency, while less toxic to people and animals, It is environmentally friendly, with other chemical agent no interactions resistances, there is preferable uptake and translocation effect, lasting effect is strong.It, which is sterilized, makees With the biosynthesis that mechanism is by interfering disease fungus cell wall chitin, inhibit the sprouting of pathogen spore, the life of mycelia Long, haustorium formation also can induce crop and generate degeneration-resistant defense reaction, achieve the effect that diseases prevention to block the sprawling of disease.

Metconazole (Metconazole) is a kind of novel, wide spectrum absorbability triazole bactericidal agent.Its mechanism of action is ergot C-14 demethylation enzyme inhibitor in sterol biosynthesis.The antifungal spectrum of metconazole is very extensive, and activity is splendid.It is main to use In prevention and treatment wheat septoria, fringe sickle-like bacteria, leaf rust, stripe rust, powdery mildew, glume blight；The short shape rust of barley, powdery mildew, beak Spore category；Rye beak genuss, leaf rust；Crown hull sugar；Triticale leaf rust, septoria musiva.It is excellent to Septoria and rust activity It is different.Have both excellent protection and therapeutic effect.It is especially effective to the glume blight of wheat, it is all good to prevent, treat effect.

Actual pesticide experience have shown that, repeat and single-minded apply a kind of reactive compound to prevent and treat harmful fungoid very It will lead to the fast selective of fungal bacterial strain in more situations, it is usual at present for the risk for reducing refractory fungal bacterial strain selectivity Harmful fungoid is prevented and treated using the mixture of different activities compound.By will have the reactive compound of different role mechanism into Row combination, can delaying drug resistance generate, reduce amount of application, reduce control cost.

Summary of the invention

The purpose of the present invention is being directed to fungicide resistance and pedo relict problem in practical applications, filters out difference and kill The fungicide of bacterium principle is compounded, and a kind of new microbicide compositions are obtained, to improve bactericidal agent for preventing and treating effect, delaying drug resistance It generates, reduces amount of application, reduce control cost.

It is a further object to provide the microbicide compositions comprising two kinds of effective constituents As and B in agriculture field Prevent and treat the application in terms of corps diseases.

The purpose of the present invention can be achieved by the following measures:

A kind of microbicide compositions, the composition include two kinds of active components of A and B, and active component A is rheum emodin first Ether or aloe-emodin, active component B are metconazole, wherein the weight ratio between two components is 10~1:1~120.

Inventor is found by experiment that composition of the invention increases for preventing and treating crops bacterium or fungal disease prevention and treatment Effect is obvious, it is often more important that amount of application is reduced, and reduces use cost.Compound structure type containing component A and component B is not Together, mechanism of action is different, and the two compounding can expand fungicidal spectrum, and can delay the production of pathogen resistance to a certain extent Raw and development speed, and no interactions resistance between component A and component B.

In a preferred embodiment, active component A and the weight ratio of active component B are 5~1:1~110, another preferred In scheme, the weight ratio of active component A and active component B are 5~1:1~100.

In a kind of preferred scheme, weight ratio between two component of A and B can arbitrarily in the range of following proportions into Row adjustment 10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1: 9、1:10、1:11、1:12、1:13、1:14、1:15、1:16、1:17、1:18、1:19、1:20、1:21、1:22、1:23、1:24、 1:25、1:26、1:27、1:28、1:29、1:30、1:31、1:32、1:33、1:34、1:35、1:36、1:37、1:38、1:39、1: 40、1:41、1:42、1:43、1:44、1:45、1:46、1:47、1:48、1:49、1:50、1:51、1:52、1:53、1:54、1: 55、1:56、1:57、1:58、1:59、1:60、1:61、1:62、1:63、1:64、1:65、1:66、1:67、1:68、1:69、1: 70、1:71、1:72、1:73、1:74、1:75、1:76、1:77、1:78、1:79、1:80、1:81、1:82、1:83、1:84、1: 85、1:86、1:87、1:88、1:89、1:90、1:91、1:92、1:93、1:94、1:95、1:96、1:97、1:98、1:99、1: 100、1:101、1:102、1:103、1:104、1:105、1:106、1:107、1:108、1:109、1:110、1:111、1:112、 1:113,1:114,1:115,1:116,1:117,1:118,1:119,1:120, can also be in two proportion institute groups of any of the above It is selected in the range of, these ratios can be understood as weight ratio, also may include molar ratio.

The dosage form allowed on pesticide can be made by active constituent and insecticides adjuvant or auxiliary material in composition of the invention.Into one Step, agriculture is made by the active component of 1~85% parts by weight and the insecticides adjuvant of 99~15% parts by weight or auxiliary material in the composition The dosage form allowed on medicine.

The present invention provides the microbicide compositions comprising component A and component B to prevent and treat corps diseases side in agriculture field The purposes in face, the especially purposes in terms of the fungi or bacterial disease for preventing and treating certain crops.

Above-mentioned composition specifically may include insecticides adjuvant or auxiliary material, such as carrier, solvent, dispersing agent, wetting agent, gluing One or more of agent, thickener, adhesive, surfactant or fertilizer etc..It can be mixed during application common Auxiliary agent.

Suitable auxiliary agent or auxiliary material can be solid or liquid, they are usually common substance during formulation, Such as natural or regenerated minerals, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive.

The method of administration of the present composition includes the aerial part that composition of the invention is used for plant growth, especially It is leaf portion or blade face.It can choose seed soaking or be applied to controlling object surface.The frequency and amount of application of application depend on pathogen Biology and weather life condition.The growth place of plant, such as rice field can be soaked with the liquid preparation of composition, or Composition is applied in soil by person in solid form, and such as in granular form (soil application), composition can be by soil through planting Object root enters in plant (systemic action).

Composition of the invention can be prepared into various dosage forms pesticide-acceptable, including but not limited to missible oil, suspension Agent, dispersible has suspending agent, water dispersible granules, pulvis, granula, aqua, aqueous emulsion, microemulsion, microcapsules at wettable powder Type, mother liquor, female powder etc., in a preferred embodiment, dosage form of the invention use water dispersible granules, aqua or suspending agent.According to The property and application composition purpose to be achieved and ambient conditions of these compositions, can choose composition to spray Mist atomizing, dusts, broadcasts sowing or sprinkles the method application poured or the like.

Composition of the invention can be prepared into various dosage forms by known method, it can be by effective component and auxiliary agent, such as Solvent, solid carrier can uniformly mix together with surfactant when needing, grind, be prepared into required dosage form.

Above-mentioned solvent can be selected from aromatic hydrocarbon, preferably contain 8-12 carbon atom, such as xylene mixture or substituted benzene, phthalein Esters of gallic acid, such as two octanoic acid of Dibutyl phthalate or phthalandione, fat hydrocarbon, such as thiacyclohexane or paraffin, the ether of pure and mild ethylene glycol and they And ester, such as ethyl alcohol, ethylene glycol, ethylene glycol monomethyl；Ketone, such as cyclohexanone, highly polar solvent, such as N- methyl -2- pyrrolidines Ketone, dimethyl sulfoxide or dimethylformamide and vegetable oil or vegetable oil, such as soybean oil.

Above-mentioned solid carrier, such as usually natural mineral fillers, such as talcum, kaolinite for pulvis and dispersible agent Soil, montmorillonite or atlapulgite.In order to manage the physical property of composition, polymolecularity silicic acid or polymolecularity can also be added Adsorpting polymerization object carrier, such as granulated adsorptive carriers or non-adsorbed carrier, suitable granulated adsorptive carriers are porous types, it is such as floating Stone, bentonite or bentonite；Suitable non-adsorbed carrier such as calcite or sand.Further, it is possible to use a large amount of inorganic in nature or having The prefabricated material to granulate of machine property is as carrier, especially dolomite.

The chemical property of effective component in composition according to the present invention, suitable surfactant are sulfomethylated lignin Acid, naphthalene sulfonic acids, phenolsulfonic acid, alkali salt or amine salt, alkylaryl sulfonates, alkyl sulfate, alkylsulfonate, fat Alcohol sulfate, fatty acid and sulphated fatty alcohol glycol ether, there are also the condensation product of sulfonated naphthalene and naphthalene derivatives and formaldehyde, naphthalene or The condensation product of naphthalene sulfonic acids and phenol and formaldehyde, NONIN HS 240, ethoxylated isooctyl-phenol, octyl phenol, nonyl phenol, Alkylaryl polyglycol ether, tributyl benzene polyglycol ether, three stearyl phenyl polyglycol ethers, alkyl aryl polyether alcohol, Ethoxylated castor oil, polyoxyethylene alkyl ether, ethylene oxide condensation product, ethoxylation polyoxypropylene, laurel acid polyethylene glycol Ether acetal, sorbitol ester, lignin sulfite waste liquor and methylcellulose.

Two kinds of effective components show as synergistic effect in composition of the invention, and the activity of the composition is than using individualized The independent activity of the expected summation of activity and single compound of closing object is more significant.Synergistic effect shows as allowing amount of application Reduction, broader antifungal control spectrum, quick, more longlasting control efficiency, by a small number of applying only once or several times more Good control plant pest fungi and broaden the possible administration interval time.These characteristics are plant epiphyte control practices It especially needs in the process.

In terms of microbicide compositions of the invention can be applied to agriculture field prevention and treatment corps diseases, farming referred herein Object disease especially uncinula necator, powdery mildew of cucumber, cucumber downy mildew, balsam pear powdery mildew, graw mold of tomato, wheat white powder Disease, rice blast.But composition of the invention is other than it can prevent and treat aforementioned disease, moreover it is possible to the specific illness of prevention and treatment include but It is not limited to wheat diseases (such as: wheat scab, wheat powdery mildew, wheat rust), rice disease (such as: rice sheath blight disease, water Rice false smut, paddy bacterial streak etc.), maize diseases (such as: smut of maize, the leaf blight of corn, corn southern leaf blight), cucumber Disease (such as: powdery mildew of cucumber, cucumber anthracnose, gray mold of cucumber, cucumber bacterial angular leaf spot), tomato disease are (such as: tomato Powdery mildew, anthracnose of tomato, tomato virus disease, tomato bacterial angular leaf spot etc.), grape disease (such as: downy mildew of garpe, grape Gray mold), apple disease (such as: ring rot of apple, apple anthracnose), citrus disease (such as: citrus scab simultaneously, citrus anthrax Disease), tobacco diseases (such as: wildfire, Alternaria alternate), rape disease (such as: sclerotinia sclerotiorum), cotton disease (such as: cotton Flower verticillium wilt, cotton wilt) etc..

Other features that bactericidal composition of the invention is shown are main are as follows: 1, composition mixture of the invention have it is obvious Synergistic effect；2, since two single dose chemical structures of this composition are widely different, the mechanism of action is entirely different, and there is no hand over Mutual resistance can delay two single doses that generated resistance problem is used alone；3, composition of the invention is to crop safety, preventive effect It is good.Tests prove that microbicide compositions chemical property of the present invention is stablized, synergy is significant, shows significantly to controlling object Synergy and complementation.

Specific embodiment

In order to make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiments, to the present invention It is further elaborated.It should be appreciated that described herein, specific examples are only used to explain the present invention, is not used to limit The fixed present invention, any modifications, equivalent replacements and improvementsmade within the spirit and principles of the invention, etc. should be included in Within protection scope of the present invention.

Percentage is weight percentage in all formulas of following embodiment.The processing work of the various preparations of the present composition Skill is the prior art, can be varied according to different situations.

One, dosage form prepares embodiment

(1) processing and embodiment of aqua

By active constituent activity grouping A and active component B, mixed with each components such as surfactant and water in the ratio of formula Conjunction is uniformly got product.

(1) active component A (Physcion) and metconazole prepare aqua

Embodiment 1:5.5% Physcion metconazole aqua (10:1)

Physcion 5%, metconazole 0.5%, styryl phenol polyethenoxy propylene ether 6%, Nonyl pheno 8%, water complements to 100%.

Embodiment 2:16.5% Physcion metconazole aqua (1:10)

Physcion 1.5%, metconazole 15%, polyoxyethylene alkyl ether 6%, sorbitol ester 8%, water complements to 100%.

Embodiment 3:49.2% Physcion metconazole aqua (1:40)

Physcion 1.2%, metconazole 48%, alkylaryl polyglycol ether 10%, ethoxylation polyoxypropylene 12%, water complements to 100%.

Embodiment 4:35.5% Physcion metconazole aqua (1:70)

Physcion 0.5%, metconazole 35%, tributyl benzene polyglycol ether 7%, ethoxylated castor oil 12%, Water complements to 100%.

Embodiment 5:60.5% Physcion metconazole aqua (1:120)

Physcion 0.5%, metconazole 60%, lauric acid polyglycol ether acetal 8%, sulphated fatty alcohol ethylene glycol Ether 15%, water complements to 100%.

(2) active component A (aloe-emodin) and metconazole prepare aqua

Embodiment 6:5.5% aloe-emodin metconazole aqua

Aloe-emodin 5%, metconazole 0.5%, remaining component are prepared according to the method for embodiment 1.

Embodiment 7:16.5% aloe-emodin metconazole aqua

Aloe-emodin 1.5%, metconazole 15%, remaining component are prepared according to the method for embodiment 2.

Embodiment 8:49.2% aloe-emodin metconazole aqua

Aloe-emodin 1.2%, metconazole 48%, remaining component are prepared according to the method for embodiment 3.

Embodiment 9:35.5% aloe-emodin metconazole aqua

Aloe-emodin 0.5%, metconazole 35%, remaining component are prepared according to the method for embodiment 4.

Embodiment 10:60.5% aloe-emodin metconazole aqua

Aloe-emodin 0.5%, metconazole 60%, remaining component are prepared according to the method for embodiment 5.

(2) processing and embodiment of water dispersible granules

Active components A and active constituent B, auxiliary agent and filler are mixed according to the formula uniformly, through air-flow crushing at can Wet powder adds a certain amount of water mixing extrusion granulation, and water dispersible granules product is made after dry screening.

(1) active component A (Physcion) and metconazole prepare water dispersible granules

Embodiment 11:9% Physcion metconazole water dispersible granules (5:1)

Physcion 7.5%, metconazole 1.5%, aromatic sulphonate 5.2%, alkyl naphthalene formaldehyde condensate sulfonate 4.6%, urea 5%, white carbon black complements to 100%.

Embodiment 12:31% Physcion metconazole water dispersible granules (1:30)

Physcion 1%, metconazole 30%, lignosulfonates 8.5%, alkylphenol-polyethenoxy formaldehyde condensation products sulphur Hydrochlorate 10%, aluminium chloride 8%, precipitated calcium carbonate complements to 100%.

Embodiment 13:76.5% Physcion metconazole water dispersible granules (1:50)

Physcion 1.5%, metconazole 75%, styryl phenol polyethenoxy polyethenoxy ether 6.3%, styrene benzene Fluosite polyoxyethylene ether phosphate salt 5.1%, aluminium-magnesium silicate 3.2%, white carbon black complements to 100%.

Embodiment 14:40.5% Physcion metconazole water dispersible granules (1:80)

Physcion 0.5%, metconazole 40%, dodecyl sulphate that 8%, copolymer-maleic anhydride sodium salt 8.2%, Bentonite 6%, kaolin complements to 100%.

Embodiment 15:60.6% Physcion metconazole water dispersible granules (1:100)

Physcion 0.6%, metconazole 60%, polymerization of carboxylic acid salt 9%, dialkyl succinylsuccinate sulfonated ester 10%, sulfuric acid Ammonium 7%, attapulgite complements to 100%.

(2) active component A (aloe-emodin) and metconazole prepare water dispersible granules

Embodiment 16:9% aloe-emodin metconazole water dispersible granules

Aloe-emodin 7.5%, metconazole 1.5%, remaining component are prepared according to the method for embodiment 11.

Embodiment 17:31% aloe-emodin metconazole water dispersible granules

Aloe-emodin 1%, metconazole 30%, remaining component are prepared according to the method for embodiment 12.

Embodiment 18:76.5% aloe-emodin metconazole water dispersible granules

Aloe-emodin 1.5%, metconazole 75%, remaining component are prepared according to the method for embodiment 13.

Embodiment 19:40.5% aloe-emodin metconazole water dispersible granules

Aloe-emodin 0.5%, metconazole 40%, remaining component are prepared according to the method for embodiment 14.

Embodiment 20:60.6% aloe-emodin metconazole water dispersible granules

Aloe-emodin 0.6%, metconazole 60%, remaining component are prepared according to the method for embodiment 15.

(3) processing and embodiment of suspending agent

By active component A and active component B, with each components such as dispersing agent, wetting agent, thickener and water in the ratio of formula It is uniformly mixed, after sand milling and/or high speed shear, obtains semi-finished product, water supplement is uniformly mixed filtering and gets product after analysis.

(1) active component A (Physcion) and metconazole prepare suspending agent

Embodiment 21:12% Physcion metconazole suspending agent (1:1)

Physcion 6%, metconazole 6%, lauryl sodium sulfate 5.3%, benzene sulfonic acid sodium salt 4%, ammonium sulfate 2%, Cyclohexanone 10%, xanthan gum 1.1%, organic silicon defoamer 0.4%, water complements to 100%.

Embodiment 22:25.2% Physcion metconazole suspending agent (1:20)

Physcion 1.2%, metconazole 24%, alkylphenol-polyethenoxy formaldehyde condensation products sulfate 6.8%, lignin Sulfonate 7.2%, urea 4%, xanthan gum 1.4%, methanol 12%, natural oil 0.8%, water complements to 100%.

Embodiment 23:42.7% Physcion metconazole suspending agent (1:60)

Physcion 0.7%, metconazole 42%, fatty acid amide N methyl taurine sodium salt 8.5%, spaonin powder 6%, second Glycol 8%, methanol 10%, aluminium-magnesium silicate 2.5%, polyethers defoaming agent 1.3%, water complements to 100%.

Embodiment 24:54.6% Physcion metconazole suspending agent (1:90)

Physcion 0.6%, metconazole 54%, alkyl phenol polyoxyethylene ether 6.3%, polyoxyethylene tri-styryl phenyl Phosphate 4.2%, ammonium sulfate 8%, soybean oil 10%, hydroxymethyl cellulose 1.8%, higher alcohols 1.6%, water complements to 100%.

Embodiment 25:33.3% Physcion metconazole suspending agent (1:110)

Physcion 0.3%, metconazole 33%, lignin sulfonic acid sodium salt 5.7%, lauryl sodium sulfate 7.8%, third Triol 10%, cyclohexanone 10%, aluminium-magnesium silicate 1.5%, higher alcohols 1.1%, water complements to 100%.

(2) active component A (aloe-emodin) and metconazole prepare suspending agent

Embodiment 26:12% aloe-emodin metconazole suspending agent

Aloe-emodin 6%, metconazole 6%, remaining component are prepared according to the method for embodiment 21.

Embodiment 27:25.2% aloe-emodin metconazole suspending agent

Aloe-emodin 1.2%, metconazole 24%, remaining component are prepared according to the method for embodiment 22.

Embodiment 28:42.7% aloe-emodin metconazole suspending agent

Aloe-emodin 0.7%, metconazole 42%, remaining component are prepared according to the method for embodiment 23.

Embodiment 29:54.6% aloe-emodin metconazole suspending agent

Aloe-emodin 0.6%, metconazole 54%, remaining component are prepared according to the method for embodiment 24.

Embodiment 30:33.3% aloe-emodin metconazole suspending agent

Aloe-emodin 0.3%, metconazole 33%, remaining component are prepared according to the method for embodiment 25.

Two, drug effect verification test

(1) Assays Example

The incidence that whole strain blade is investigated according to test grade scale, calculates disease index and control efficiency.

Control efficiency is converted into probability value (y), liquor strength (μ g/ml) is converted into logarithm (x), with least square method Concentration EC50 in calculating virulence equation and inhibiting, the toxicity index and co-toxicity coefficient (CTC) of medicament are calculated according to Sun Yunpei's Method.

Survey toxicity index (ATI)=(standard agent EC50/ reagent agent EC50) * 100

Percentage composition+B medicament toxicity index * the mixture of A in theoretical toxicity index (TTI)=A medicament toxicity index * mixture The percentage composition of middle B

Co-toxicity coefficient (CTC)=[mixture surveys toxicity index (ATI)/mixtures theoretical toxicity index (TTI)] * 100

CTC≤80, composition show as antagonism, 80 < CTC < 120, and composition shows as summation action, and CTC >= 120, composition shows as synergistic effect.

1, Physcion is compounded with metconazole tests apricot scab toxicity test

1. Physcion of table is compounded with metconazole to apricot scab toxicity test interpretation of result

As a result (table 1) shows in Physcion: in metconazole=10~1:1~120 interval ranges, Physcion It is compounded with metconazole and the control efficiency of apricot scab is significantly improved, illustrate that the two compounding has significantly apricot preventing and controlling black spot Synergistic effect.

2, aloe-emodin is compounded with metconazole tests cucumber timberrot toxicity test

2. aloe-emodin of table is compounded with metconazole to cucumber timberrot toxicity test interpretation of result

Medicament title	EC50(ug/mL)	ATI	TTI	CTC
					Aloe-emodin	4.86	100.00	\	\
Metconazole	5.72	84.97	\	\
					Aloe-emodin: metconazole=20:1	4.22	115.28	99.28	116.11
Aloe-emodin: metconazole=10:1	3.82	127.30	98.63	129.06
					Aloe-emodin: metconazole=5:1	3.65	133.05	97.50	136.47
Aloe-emodin: metconazole=1:1	3.70	131.50	92.49	142.18
					Aloe-emodin: metconazole=1:10	3.65	133.15	86.34	154.22
Aloe-emodin: metconazole=1:20	3.35	145.11	85.69	169.35
					Aloe-emodin: metconazole=1:30	3.05	159.43	85.45	186.57
Aloe-emodin: metconazole=1:40	2.83	171.82	85.34	201.34
					Aloe-emodin: metconazole=1:50	2.94	165.43	85.26	194.02
Aloe-emodin: metconazole=1:60	3.12	155.60	85.22	182.59
					Aloe-emodin: metconazole=1:70	3.29	147.52	85.18	173.18
Aloe-emodin: metconazole=1:80	3.45	140.68	85.16	165.20
					Aloe-emodin: metconazole=1:90	3.71	131.15	85.14	154.05
Aloe-emodin: metconazole=1:100	3.94	123.41	85.12	144.98
					Aloe-emodin: metconazole=1:110	4.22	115.07	85.11	135.21
Aloe-emodin: metconazole=1:120	4.58	106.05	85.09	124.63
					Aloe-emodin: metconazole=1:130	4.94	98.34	85.08	115.58

As a result (table 2) shows that aloe-emodin is compounded with metconazole and significantly improves to the control efficiency of cucumber timberrot poison, Illustrate that the two compounding has significant synergistic effect to cucumber timberrot prevention and treatment.

(2) field efficacy verification test

Test method: it at the initial stage of a disease, carries out for the first time by spraying, carrying out being administered for second after 7 days immediately, each processing 4 A cell, each 20 square meter of cell.11 days investigation statistics incidences, 5 points of each cell are random before medicine and after second of medicine Sampling, 5 plants of crops of every investigation, the lesion area for investigating every blade in whole strain account for percentage and the classification of blade area, calculate Disease index and control efficiency.

It is expected that preventive effect (%)=X+Y-XY/100 (wherein, X, Y are single dose preventive effect)

Grade scale:

0 grade: disease-free spot；

1 grade: leaf spot lesion is less than 5, and length is less than 1cm；

3 grades: leaf spot lesion 6-10, part scab length is greater than 1cm；

5 grades: leaf spot lesion 11-25, part scab is linked to be piece, and lesion area accounts for the 10-25% of leaf area；

7 grades: leaf spot lesion 26 or more, scab is linked to be piece, and lesion area accounts for the 26-50% of leaf area；

9 grades: scab is linked to be piece, lesion area account for 50% of leaf area or more or full leaf it is withered.

1, Physcion compounds field efficacy experiment with metconazole

3 Physcion of table and metconazole mixture are to lettuce controlling powdery mildew effect

Measurement result (table 3) shows that Physcion significantly improves lettuce powdery mildew preventive effect with compounding for metconazole, says Both bright compounding has significant synergistic effect to lettuce powdery mildew.

2, aloe-emodin compounds field efficacy experiment with metconazole

4 aloe-emodin of table and metconazole mixture are to soybean rust control efficiency

Measurement result (table 4) shows that aloe-emodin is significantly improved with preventive effect of the metconazole mixture to soybean rust, explanation The two compounding has significant synergistic effect to soybean rust.

Claims (8)

1. a kind of microbicide compositions, which is characterized in that the composition includes two kinds of active components of A and B, active component A For Physcion or aloe-emodin, active component B is metconazole, wherein the weight ratio between two components be 10~1:1~ 120。

2. microbicide compositions according to claim 1, which is characterized in that the weight ratio of active component A and active component B For 5~1:1~110.

3. microbicide compositions according to claim 2, which is characterized in that the weight ratio of active component A and active component B For 5~1:1~100.

4. microbicide compositions according to any one of claims 1 to 3, which is characterized in that the composition is by activity The dosage form allowed on pesticide is made in ingredient and insecticides adjuvant or auxiliary material.

5. microbicide compositions according to claim 4, which is characterized in that the composition by 1~85% parts by weight work Property component and 99~15% parts by weight insecticides adjuvant or auxiliary material the dosage form allowed on pesticide is made.

6. microbicide compositions according to claim 4 or 5, which is characterized in that the dosage form is aqua, water-dispersible grain Agent or suspending agent.

7. microbicide compositions according to claim 4 or 5, which is characterized in that the insecticides adjuvant or auxiliary material are selected from and carry One or more of body, solvent, dispersing agent, wetting agent, adhesive, thickener, adhesive, surfactant or fertilizer.

8. use of the microbicide compositions described in claim 1~7 any one in terms of agriculture field prevents and treats corps diseases On the way.