CN109456926A - A kind of microbial bacterial agent and its application containing thermophilic salt denitrifying bacterium YL5-2 - Google Patents
A kind of microbial bacterial agent and its application containing thermophilic salt denitrifying bacterium YL5-2 Download PDFInfo
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Abstract
The invention discloses a kind of microbial bacterial agent containing thermophilic salt denitrifying bacterium YL5-2 and its applications.A kind of microbial bacterial agent is the new species that Halovibrio belongs to containing thermophilic salt denitrifying bacterium YL5-2, YL5-2, is preserved in China General Microbiological culture presevation administrative center, and deposit number is CGMCC NO.16315, and the deposit date is on August 20th, 2018.Application of the microbial bacterial agent of the present invention in high-salt wastewater processing.YL5-2 can be used for the biological denitrificaion processing of high-salt wastewater of the salt content greater than 10%, reduce the denitrogenation processing cost of such waste water.The microbial bacterial agent of thermophilic salt denitrifying bacterium YL5-2 preparation disclosed by the invention, it can be used for the degradation, conversion of pollutant and biological denitrification process under high salt conditions, including high-salt wastewater processing, polluted seawater is administered, salt-soda soil is repaired, and nitrogen nutrition is consumed, and inhibits algae excessive propagation, purifying water body, improvement substrate etc..
Description
Technical field
The present invention relates to environmentally friendly microorganism fields, more particularly to a kind of microbial bacteria containing thermophilic salt denitrifying bacterium YL5-2
Agent and its application.
Background technique
Polluted by nitrogen is the one of the major reasons for causing water eutrophication.The denitrogenation processing of waste water is for maintaining water environment matter
It measures and prevents water eutrophication from playing a significant role.The nitrogenous effluent of the industries such as petroleum, chemical industry, food processing, chemical fertilizer discharge
Have the characteristics that with high salt.Certain waste water that chemical industry, food processing generate, salt content are even more than seawater.At traditional biological method
Managing Low-salinity nitrogenous effluent has advantage, but when waste strength is excessively high, can inhibit the metabolism of denitrification microorganism.Salt tolerant and
The presence of the denitrifier of thermophilic salt provides inner theoretic possibility for high-salt wastewater biological denitrificaion.
Biological denitrificaion bacterium bag includes nitrifier and denitrifying bacterium.Biological denitrificaion bacterium under the conditions of high-salt wastewater can also be divided into nitre
Change bacterium and denitrifying bacterium.It can be the bacterium of gaseous nitrogen compound by nitrate or pressure nitrate reduction that denitrifying bacterium, which is a kind of,.
Therefore, screening separates and cultivates nitrifier and the denitrifying bacterium of salt tolerant and thermophilic salt from environment, and it is raw just to become solution high-salt wastewater
The key of object Denitrogenation.
Dalian Ocean University's focus et al. (the separation identification of the thermophilic salt denitrifying bacterium of mono- plant of moderate of such as focus, flower bud and its generation
It thanks characteristic research " aquatic science and technology information ", 2018,45 (3): 149~154) being separated from mariculture purification of waste water unit
To the thermophilic salt denitrifying bacterium of one plant of moderate, belong to Halomonas category, best metabolism growth condition is 30 DEG C of temperature, salinity 100g/
L, pH7.5~8.5, C/N ratio are 4:1.
University Of Qingdao Guo gorgeous equal (the separation identification of mono- plant of the such as Guo Yanli, Zhang Peiyu slight thermophilic salt denitrifying bacterium and spy
Property " application and environmental organism journal " 2010,16 (3): 394~398) separated from the mature activated sludge of processing high-salt wastewater
Obtain one plant of slight thermophilic salt aerobic denitrifying bacteria YL-1, belong to enlightening thatch Salmonella (Dietzia sp.), the bacterium can 0%~
10%, pH 7.5~8.5, utilize acetic acid, sucrose, glucose, sodium citrate, sodium succinate carry out denitrification.
Nanjing University of Technology slanders minor benefit and (slanders separation identification and its Denitrification Characteristics research of the thermophilic salt denitrifying bacteria of minor benefit
Nanjing University of Technology's master's thesis in 2013) by only nitrogen source of sodium nitrate and the training of heterotrophic denitrification that salinity is 8%
Support base, be enriched with, separate and screening has obtained 6 plants of thermophilic salt denitrifying bacteriums from the pedotheque in Yancheng saltern: NY-1, NY-11 with
NY-13 is branch bacillus, and (Virgibacillus sp.), NY-8 and NY-10 are Halomonas (Halmonas
Sp.), NY-4 is marinobacter (Marinobacter sp.), and salinity growth scope is 0%~12%, the most suitable growth salt
Concentration is 3%~8%.Wherein the denitrifying capacity of NY-4 is most strong, using trisodium citrate as carbon source, salinity 8%, C/N 5,
When pH is 8, NO3The removal rate of-N is 95%.
The prior art is less about the report of thermophilic salt denitrifying bacterium, the anti-nitre of thermophilic salt under conditions of especially salinity > 10%
Change bacterium.It is the skill that such wastewater biological denitrificaion needs to solve that acquisition salt resistance ability, which is greater than 10% thermophilic salt denitrifying bacterium, from environment
Art problem.
Summary of the invention
Under the conditions of to solve the problems, such as the biological denitrificaion under high salt conditions, especially salinity greater than 10%
High-salt wastewater biological denitrificaion problem, a kind of microbial bacterial agent containing thermophilic salt denitrifying bacterium YL5-2 is provided.
It is a further object of the present invention to provide the applications of the microbial inoculum.
What the purpose of the present invention can be achieved through the following technical solutions:
A kind of microbial bacterial agent is the new species that Halovibrio belongs to, preservation containing thermophilic salt denitrifying bacterium YL5-2, YL5-2
In China General Microbiological culture presevation administrative center, deposit number is CGMCC NO.16315, and the deposit date is Augusts 20 in 2018
Day.
The login of the GenBank/EMBL/DDBJ of the 16S rRNA sequence of thermophilic salt denitrifying bacterium YL5-2 disclosed by the invention
Number be MF782425, nucleotide sequence is as shown in SEQ ID NO.1；The GenBank/EMBL/DDBJ's of whole genome sequence steps on
Record number is NSKD00000000.1.
Thermophilic salt denitrifying bacterium YL5-2 disclosed by the invention is Gram-negative, facultative aerobic, direct rod shape or 0.5~0.8 μm
× 1.0~3.5 μm of small vibrios character, and by unipolarity flagellum movement, the bacterium colony on solid medium be it is smooth and
It is light yellow.
Thermophilic salt denitrifying bacterium YL5-2 disclosed by the invention can be in salinity 3%~32%, pH6.5~11.0, temperature 15
It is grown within the scope of~45 DEG C.When using acetic acid as carbon source, NO3- N or NO2When-N is electron acceptor, the most suitable growth salt environment of YL5-2
It is 5%~25%, the most suitable growth pH is 7.5~8.0, optimum growth temperature is 30~35 DEG C.
Thermophilic salt denitrifying bacterium disclosed by the invention, as dissolved oxygen < 1.0mg/L (anaerobic condition), can with acetic acid or its
Its organic matter is that electron donor carries out denitrification；As dissolved oxygen < 0.5mg/L (anaerobic condition), with acetic acid or other can have
Machine object is that electron donor carries out denitrification；It, can be using acetic acid or other organic matters as electron donor as dissolved oxygen >=1.0mg/L
Carry out denitrification；As dissolved oxygen >=2.0mg/L, denitrification can be carried out using acetic acid or other organic matters as electron donor.
Microbial bacterial agent of the present invention is preferably composite bacteria agent, also contains other wastewater treatment bacterium.
Application of the microbial bacterial agent of the present invention in high-salt wastewater processing.
Microbial bacterial agent of the present invention is preferably in the biological denitrificaion processing of the high-salt wastewater of salinity 5%~25%
Using.
Microbial bacterial agent of the present invention further preferably under anoxic conditions or under aerobic condition is greater than salinity
10% high-salt wastewater carries out denitrification.
Using bacterial strain YL5-2 of the present invention prepare microbial bacterial agent, when using biological denitrificaion be main target progress in application,
The resistance to salt concentration range that is most preferably applicable in of the microbial inoculum is 5%~25%.
(1) when waste water salinity is 5%~10%, which can be with NO3- N or NO2- N is used as electron acceptor, and TN≤
100mg/L, C/N >=5, and pass through 72h, NO3- N or NO2The removal rate of-N can achieve 90% or more.
(2) when waste water salinity is 10%~20%, which can be with NO3- N or NO2- N is used as electron acceptor, and TN
≤ 100mg/L, C/N >=5, and pass through 72h, NO3- N or NO2The removal rate of-N can reach 95% or more.
(3) when waste water salinity is 20%~30%, which can be with NO3- N or NO2- N is used as electron acceptor, and TN
≤ 100mg/L, C/N >=5, and pass through 72h, NO3- N or NO2The removal rate of-N can reach 95% or more.
Beneficial effects of the present invention:
(1) present invention finds the new species that a Halovibrio belongs to, it is named as Halovibrio salipaludis
sp.nov.The bacterial strain is a kind of facultative or aerobic denitrifying bacteria, and salt tolerant range is 3%~32%, can salinity 5%~
With NO in 25% range3- N and NO2Denitrification is carried out as electron acceptor.The microbial bacterial agent prepared using YL5-2, can be used for
The biological denitrificaion processing of high-salt wastewater of the salt content greater than 10%, reduces the denitrogenation processing cost of such waste water.The present invention discloses
Thermophilic salt denitrifying bacterium YL5-2 preparation microbial bacterial agent, can be used for the degradation, conversion of pollutant and biology under high salt conditions
Denitrification process, including high-salt wastewater processing, polluted seawater are administered, and salt-soda soil is repaired, and consume nitrogen nutrition, inhibit algae excessive numerous
It grows, purifying water body, improvement substrate etc..
Detailed description of the invention:
Cell after the thermophilic salt denitrifying bacterium YL5-2 cell of Fig. 1 is cultivated for 24 hours under the conditions of on YL agar plate in 37 DEG C transmits
Electron microscopic is according to (scale bar is 2 μm)
Fig. 2 is thermophilic salt denitrifying bacterium YL5-2 and Halovibrio variabilis DSM 3050T and other relationship kind structures
The systematic growth tree graph based on 16S rRNA built.The figure is for describing thermophilic salt denitrifying bacterium YL5-2 and Halovibrio
Denitrificans DSM15503 and other kinds.Digital representation the value of the confidence > 50% (1000 at phylogenetic tree branch point
Secondary repetition).Scale indicates have 5 to be replaced in 100 nucleotide.By Pontibacter mucosus PB3TAs outer class
Group.Every kind of bacterial strain all designates GenBank accession number.
Fig. 3 is thermophilic salt denitrifying bacterium YL5-2 and Halovibrio variabilis DSM 3050T and other relationship strains
The systematic growth tree graph of maximum parsimony method (MP) building based on 16S rRNA.The figure is for describing thermophilic salt denitrifying bacterium YL5-2
With the relationship between the taxon of Halovibriovariabilis DSM 3050T and other strains.Marinobacter
Oulmenensis Set74T (AY130994) is used as outgroup.It is GenBank accession number in bracket.Phylogenetic tree branch point
Digital representation the value of the confidence > 50% (1000 repetitions) at place
Biomaterial preservation information
YL5-2, classification naming is Halovibrio salipaludis, the deposit date is on August 20th, 2018, preservation list
Position is China General Microbiological culture presevation administrative center, and preservation address is Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3 China
Institute of microbiology, the academy of sciences, deposit number: CGMCC NO.16315.
Specific embodiment
The separation and preservation of the thermophilic salt denitrifying bacterium of embodiment 1
Thermophilic salt denitrifying bacterium YL5-2 is deposited from Qinghai Province Golmud Cha Er Han Salt Lake (36 ° of 51 ' N, 94 ° of 95 ' E)
It is isolated in soil.Cha Er Han Salt Lake lake water is saturation salinity or close saturation salinity throughout the year.
It configures NaCl concentration and is 20% LB liquid medium, and glycerol 250mg/L, glucose 250mg/L, methanol is added
50mg/L, to Cha Er Han Salt Lake sedimentary soil enrichment culture 72h under the conditions of 30 DEG C.Using YL solid medium to enrichment culture
Bacterial strain in liquid is separated.Contain following components in 1L culture medium: glucose: 0.6g, trisodium citrate 0.5g, glycerol 2mL,
Yeast extract 0.8g, peptone 1.6g, dipotassium hydrogen phosphate 0.35g, potassium dihydrogen phosphate 0.1g, ammonium sulfate 0.25g, ammonium chloride
0.25g, MgSO40.5g, CaCl20.1g, NaCl 180g；Microelement SL-4 10mL, pH 7.0-7.2；Agar 2.5%.
Bacterial strain YL5-2 is deposited in China General Microbiological culture presevation administrative center, and deposit number is CGMCC NO.16315,
The deposit date is on August 20th, 2018.
The analysis of the thermophilic salt denitrifying bacterium YL5-2 16S rRNA sequence of embodiment 2 and full gene sequencing and analysis
Bacterial strain YL5-2 extracting genome DNA uses TaKaRa kit (TaKaRa MiniBEST Bacteria
Genomic DNA Extraction 68Kit Ver.3.0)。
16S rRNA amplification uses universal bacterial primer 2 7F (5 '-AGAGTTTGATCMTGGCTCA G-3 ') and 1492R
(5'-TACGGYTACCTTGTTACGACTT-3').PCR sequencing commission Shanghai Sheng Gong Biotechnology Co., Ltd carries out.Bacterial strain
The complete 16S rRNA sequence of YL5-2 is 1518bp, and as shown in SEQ ID NO.1, GenBank accession number is MF782425.
The genome sequencing of bacterial strain YL5-2 is using Shanghai's style Sen Nuo Biotechnology Co., Ltd Illumina on Shanghai
2000 high-flux sequence platform of MiSeq.Raw sequencing data is filtered using PRINSEQ (version number v 0.20.4) software
And amendment, the base of genome is then carried out using SOAP denovo software (version number v1.05) software with default parameters
Pairing, then using the integrality of CheckM software (version 1.03) assessment genome.Protein coding open reading frame is adopted
It is predicted with Glimmer software (version number 1.2).RNA prediction uses RNAmmer software (version 1.2).Bacterial strain YL5-2 is complete
Totally 3,495,096bp, GenBank accession number is NSKD00000000.1 to genome sequence.
DNA-DNA cross experiment passes through bacterial strain YL5-2 and the immediate vibrio Halovibrio mode of its genetic development
Bacterial strain carries out.This method is equal to 1970 by De Ley to be proposed, DNA hybridization value (dDDH) uses the 2nd kind of mode (version of GGDC software
This number comparison one by one for 2.0) carrying out gene order obtains.DNA test and analysis the result shows that, bacterial strain YL5-2 and
Halovibrio variabilis DSM 3050T、Halovibrio denitrificans DSM15503TDNA-DNA it is miscellaneous
Friendship value is respectively 43.5% and 38.2%, far below 70% threshold value (species divide generally accepted threshold value).
Nucleotide average homogeneity value carries out 1000 repetition topology verifications using the base group of whole genome sequence and obtains.
This method is equal to 2007 by Goris and proposes, the software used is MUMmer (version number 3.23) and Jspecies (version number
1.2.1).Based on the ANI threshold range (95-96%) that Kim et al. and Richter et al. species proposed divide, to bacterial strain
Genome closely related therewith carries out ANI analysis in the genome and GenBank of YL5-2.The result shows that bacterial strain YL5-2 its
It is 88.5%, this is bacterial strain with average nucleotide identity (ANI) value highest of Tamilnaduibacter alinus Mi 7
YL5-2TA kind of new species for belonging to Halovibrio category provide argument, are named as Halovibrio salipaludis
sp.nov。
The phenotypic characteristic and physiological and biochemical property of the thermophilic salt denitrifying bacterium YL5-2 of embodiment 3 is identified
Gram's staining characteristic is tested using BD Gram's staining kit.
Cell mobility is measured using half MA culture medium (0.5% agar, w/v).
Cellular morphology uses transmission electron microscope (TEM) analysis detection.I.e. picking is thin from the culture solution of exponential growth
Born of the same parents, with 0.5% uranyl acetate staining cell, and it is right at microscope (Tecnai Spirit, FEI, Hillsboro, OR, USA)
Cell is taken pictures.
Oxidase active uses oxidase reagent box (bioM é rieux), by by 3.0%H2O2Solution pours into bacterial clump
And it observes bubble and generates to measure catalase activity.
Temperature growth condition carries out on YL liquid agar medium, temperature is respectively 4,10,15,20,25,30,33,
37,40,45 and 50 DEG C, pH constant is 7.5, compares bacterial strain YL5-2 under different temperaturesTGrowth rate determine its optimum growh temperature
Degree.
YL agar and YL fluid nutrient medium of the salt resistance ability in 0.0-30.0%NaCl (w/v) carry out.Use buffer
(Na2HPO4/NaH2PO4(pH 5.0-7.0), Na2CO3/NaHCO3(pH 8.0-12.0)) pH is adjusted to 5.0,5.5,6.0,
7.0,8.0,9.0,10.0 and 11.0 (15.0%NaCl, 35 DEG C) are to measure the pH range for being suitble to growth.
Utilization of carbon source ability and enzymatic activity test use API 20NE, API ZYM (bioM é rieux) and Biolog
GENIII microwell plate.The cell of pregrown on all test inoculation YL culture mediums, and diluted with relevant inoculation medium.
The phenotypic characteristic and physiological and biochemical property qualification result of bacterial strain YL5-2 is as shown in table 1:
1 halophilic vibrio Halovibrio sp.YL5-2 (a) of table and Halovibrio denitrificans DSM15503T
(b), Halovibrio variabilis DSM 3050T(c) phenotype in terms of distinguishing characteristics compared with
Illustrate :+, it is positive；, negative.
Thermophilic salt denitrifying bacterium YL5-2 is that Gram-negative, alkalinity are aerobic, direct rod shape or 0.5-0.8x1.0-3.5 μm
Small vibrios shape, and pass through unipolarity flagellum movement (Fig. 1).
Bacterial strain YL5-2 is grown under aerobic condition using acetic acid, then grows (API using nitrate under anoxic conditions
20NE)。
The fermented and cultured of the thermophilic salt denitrifying bacterium YL5-2 of embodiment 4 is tested
(1) medium component is glycerol 500mg/L, glucose 250mg/L, methanol 500mg/L, methylamine 200mg/L, chlorination
100~250g/L of sodium, sodium acetate 250mg/L, trisodium citrate 250mg/L, yeast powder 100mg/L, peptone 200mg/L, ox
Meat extract 200mg/L, microelement is a small amount of, and pH is 7.5~8.0.Fluid nutrient medium sterilizes, while controlling moisture evaporation.
(2) 48h is cultivated under the conditions of 35 DEG C after being inoculated in the triangular flask of 1L, the lost influence to keep the skin wet in incubation
The variation of salinity.Measured after 48h OD600 under 10%, 15%, 20%, 25% salt concentration conditions be respectively 1.72,1.65,
1.62,1.60,1.63.Switching culture twice is carried out, cultivates 48h after switching every time, OD600 is respectively after the completion of domestication culture
2.56、2.68、2.72、2.68、2.52。
(3) 1L culture solution, which is inoculated into the aerobic fermentation tank of 20L, is cultivated, and medium component remains unchanged.Still it trains
48h is supported, cultivation temperature is 35 DEG C, mixing speed 100rpm, and dissolved oxygen is 2.0~4.0mg/L.Bacterium solution in 48h post-fermentation tank
OD600 can reach between 2.6~3.0.
(4) it examines: sampling observation daily using microscope in incubation, check whether there is miscellaneous bacteria and be mixed into growth；Simultaneously
Observe the growth and metamorphosis situation of YL5-2.
(5) result: test result shows that bacterial strain YL5-2 can be rapidly performed by fermented and cultured and amplification, this shows YL5-
2 potentiality with large-scale engineering applications.
The salt resistance ability of the thermophilic salt denitrifying bacterium YL5-2 of embodiment 5 is tested
(1) medium component is glycerol 50mg/L, glucose 25mg/L, methanol 50mg/L, methylamine 20mg/L, sodium chloride
250g/L, sodium acetate 25mg/L, trisodium citrate 25mg/L, yeast powder 10mg/L, peptone 20mg/L, beef extract 20mg/L,
Agar 20g/L.
(2) fresh above-mentioned culture medium activated spawn is used, is enriched to lawn growth within culture 3 days spare
(3) salinity gradient is arranged: according to the enrichment isolation condition of YL5-2 strain, the salinity gradient and model of culture medium is arranged
Enclose is 0%, 0.5%, 1%, 2%, 3%, 24%, 26%, 28%, 30%, 32%, 34%.
(4) it prepares culture medium: culture medium being prepared according to the culture medium prescription of setting, the higher culture medium hot water of salinity melts
After sterilize, every bottle of evaporation water for adding 5ml is added after volatile medium component is subject to sterilization and shakes up, is cooled to 60 DEG C of left sides
The right side, plate processed, salinity height easily solidify, and therefore, plate processed is quickly fast (cold when 32% salinity medium plate
But have after solidifying and salt out on a small quantity, 34% salinity there are a large amount of salt crystals to be precipitated).
(5) be inoculated with: the fresh lawn of one ring of aseptic condition picking accesses each salinity medium, from Low-salinity toward high salinity gradient
Switching has been inoculated with culture dish and has been sealed with sealed membrane as scribing line track has a large amount of salt crystals to be precipitated after the scribing line of 34% salinity medium
Mouthful, 35-37 DEG C culture 3-7 days, observation growth situation.
(6) test result: YL5-2 strain salt tolerant range is 3-32%.YL5-2 bacterial strain is three in 3-30% salinity medium
Can obviously observe the lawn newly grown in it, but grow 7 days in 32% saturation salinity medium or more can just grow
Visually visible lawn.Show that YL5-2 speed of growth in 3%~30% salt concentration range is very fast.
Growing state of 2 YL5-2 of table on different salinity culture mediums
The salt tolerant denitrifying capacity of the thermophilic salt denitrifying bacterium YL5-2 of embodiment 6 is tested
(1) culture medium: acetic acid 2000mg/L, peptone 20mg/L, beef extract 20mg/L, NO3- N is 100mg/L, NaCl
Concentration 3%~30%, buffer, which is added, makes pH 7.5~8.0.
(2) experimental design: carrying out in the triangular flask of 10 500mL of denitrification test, each that culture medium 300mL, setting 8 is added
A salinity gradient, salt content is respectively 3%, 6%, 10%, 12%, 15%, 20%, 25%, 30%, wherein salt-free blank
Group is also provided with 2 in parallel.
(3) denitrification is tested: being inoculated with YL-5 culture solution about 10mL after all samples sterilizing, is trained on constant-temperature table
It supports, temperature is 30~35 DEG C；Shaking speed is respectively 10ppm；It is measured by sampling in triangular flask respectively at for 24 hours, after 48h and 72h
NO3The concentration of-N.Test result such as following table data:
3 YL5-2 of table denitrification under different salinity removes NO3The test result (unit: mg/L) of-N
| 0 | 0 | 3% | 6% | 10% | 12% | 15% | 20% | 25% | 30% | |
| 24h | 99.6 | 99.5 | 91.3 | 76.2 | 68.8 | 67.5 | 66.4 | 67.6 | 72.6 | 89.6 |
| 48h | 99.1 | 99.2 | 55.4 | 18.6 | 14.4 | 11.7 | 9.9 | 14.7 | 19.1 | 40.6 |
| 72h | 98.5 | 98.8 | 13.5 | 6.7 | 4.5 | 3.6 | 3.8 | 4.2 | 8.2 | 12.5 |
The salt tolerant denitrifying capacity of the thermophilic salt denitrifying bacterium YL5-2 of embodiment 7 is tested
(1) culture medium: acetic acid 2000mg/L, peptone 20mg/L, beef extract 20mg/L, NO2- N is 100mg/L, NaCl
Concentration 3%~30%, buffer, which is added, makes pH 7.5~8.0.
(2) experimental design: carrying out in the triangular flask of 10 500mL of denitrification test, each that culture medium 300mL, setting 8 is added
A salinity gradient, salt content is respectively 3%, 6%, 10%, 12%, 15%, 20%, 25%, 30%, wherein salt-free blank
Group is also provided with 2 in parallel.
(3) denitrification is tested: being inoculated with YL-5 culture solution about 10mL after all samples sterilizing, is trained on constant-temperature table
It supports, temperature is 30~35 DEG C；Shaking speed is respectively 10ppm；It is measured by sampling in triangular flask respectively at for 24 hours, after 48h and 72h
NO2The concentration of-N.Test result such as following table data:
4 YL5-2 of table denitrification under different salinity removes NO2The test result (unit: mg/L) of-N
| 0 | 0 | 3% | 6% | 10% | 12% | 15% | 20% | 25% | 30% | |
| 24h | 99.7 | 99.7 | 95.3 | 86.2 | 78.8 | 77.5 | 76.4 | 77.6 | 81.6 | 91.6 |
| 48h | 99.3 | 99.1 | 62.4 | 20.3 | 16.5 | 14.8 | 11.3 | 15.7 | 19.1 | 30.6 |
| 72h | 99.0 | 98.6 | 15.8 | 9.7 | 6.6 | 4.2 | 3.8 | 7.3 | 8.5 | 15.3 |
The microbial bacterial agent that embodiment 8 is prepared using thermophilic salt denitrifying bacterium YL5-2
(1) medium component is glycerol 500mg/L, glucose 250mg/L, methanol 500mg/L, methylamine 200mg/L, acetic acid
Sodium 250mg/L, trisodium citrate 250mg/L, yeast powder 100mg/L, peptone 200mg/L, beef extract 200mg/L, micro member
Plain a small amount of, pH is 7.5~8.0；For the degradation effect of YL5-2 under more different salinity, sodium chloride concentration is prepared in culture medium
At 3%, 6%, 10%, 15%, 20%, 25%, 30%.Fluid nutrient medium sterilizes, while controlling moisture evaporation.Inoculation
Halophilic vibrio Halovibrio sp.YL5-2 carries out repeatedly passing on amplification cultivation under the conditions of 35 DEG C, and single cultivation cycle is
60h controls OD600 > 1.0 after culture.
(2) bacterium solution cultivated is halophilic vibrio Halovibrio sp.YL5-2 microbial bacterial agent.
(3) using the microbial inoculum handled under aerobic condition TDS be respectively 3%, 6%, 10%, 15%, 20%, 25%,
30%, acetic acid is the waste water of 1000mg/L, after 72h the degradation rate of acetic acid be respectively 88.3%, 91.2%, 91.6%, 92.3%,
92.2%, 91.3%, 88.2%.
(4) microbial inoculum is handled under 35 DEG C, anoxia condition TDS be respectively 3%, 6%, 10%, 15%, 20%, 25%,
30%, acetic acid 1000mg/L, NO3- N≤100mg/L waste water, after 48h the degradation rate of acetic acid be respectively 81.3%,
88.2%, 89.5%, 89.6%, 89.3%, 87.1%, 81.9%.
(5) being used to handle TDS for the microbial inoculum is respectively 3%, 6%, 10%, 15%, 20%, 25%, 30%, and acetic acid is
1000mg/L、NO2- N≤100mg/L waste water, 48h acetic acid degradation rate is respectively 86.5%, 88.3%, 92.2%, 91.8%,
90.7%, 83.8%.
(6) by the microbial inoculum be used to handle TDS be respectively 3%, 6%, 10%, 15%, 20%, 25%, 30%, COD≤
3000mg/L、NO3The waste water of≤100mg/L, NO after 72h3- N degradation rate is respectively 84.3%, 90.2%, 93.4%, 93.6%,
91.8%, 82.2%.
The microbial bacterial agent that embodiment 9 is prepared using thermophilic salt denitrifying bacterium YL5-2
(1) medium component is glycerol 500mg/L, glucose 250mg/L, methanol 500mg/L, methylamine 200mg/L, acetic acid
Sodium 250mg/L, trisodium citrate 250mg/L, yeast powder 100mg/L, peptone 200mg/L, beef extract 200mg/L, micro member
Plain a small amount of, pH is 7.5~8.0；For the degradation effect of YL5-2 under more different salinity, sodium chloride concentration is prepared in culture medium
At 3%, 6%, 10%, 15%, 20%, 25%, 30%.Fluid nutrient medium sterilizes, while controlling moisture evaporation.Inoculation
Halophilic vibrio Halovibrio sp.YL5-2 and Halovibrio denitrificans DSM15503, then in 35 DEG C of conditions
Repeatedly passage amplification cultivation, single cultivation cycle are 72h for lower progress, and OD600 > 1.0 is controlled after culture.
(2) bacterium solution cultivated is halophilic vibrio mixed microorganism microbial inoculum.
(3) using the microbial inoculum handled under aerobic condition TDS be respectively 3%, 6%, 10%, 15%, 20%, 25%,
30%, and acetic acid is the waste water of 1000mg/L, after 72h the degradation rate of acetic acid be respectively 90.3%, 92.6%, 92.7%,
92.8%, 91.8%, 90.7%, 89.5%.
(4) microbial inoculum is handled under 35 DEG C, anoxia condition TDS be respectively 3%, 6%, 10%, 15%, 20%, 25%,
30%, and acetic acid is 1000mg/L, NO3- N≤100mg/L waste water, after 72h the degradation rate of acetic acid be respectively 84.5%,
91.2%, 91.8%, 92.3%, 92.2%, 88.5%, 83.2%.
(5) by the microbial inoculum at 30 DEG C, for handling TDS be respectively 3% under anoxia condition, 6%, 10%, 15%, 20%,
25%, 30%, and acetic acid is 1000mg/L, NO2The waste water of≤100mg/L, after 72h acetic acid degradation rate be respectively 83.6%,
90.7%, 92.0%, 91.5%, 91.9%, 84.2%.
(6) by the microbial inoculum at 25 DEG C, for handling TDS be respectively 3% under anoxia condition, 6%, 10%, 15%, 20%,
25%, 30% and COD≤3000mg/L, NO3The waste water of≤100mg/L, NO after 72h3Degradation rate is respectively 88.3%,
93.1%, 95.5%, 95.6%, 92.8%, 86.6%.
The complex micro organism fungicide that embodiment 10 is prepared using thermophilic salt denitrifying bacterium YL5-2
(1) medium component is glycerol 500mg/L, glucose 250mg/L, methanol 500mg/L, methylamine 200mg/L, chlorination
Sodium 120g/L, sodium acetate 250mg/L, trisodium citrate 250mg/L, yeast powder 100mg/L, peptone 200mg/L, beef extract
200mg/L, microelement is a small amount of, and pH is 7.5~8.0.Fluid nutrient medium sterilizes, while controlling moisture evaporation.Inoculation
Halophilic vibrio Halovibrio sp.YL5-2 and Halovibrio denitrificans DSM15503, then in 35 DEG C of conditions
Repeatedly passage amplification cultivation, single cultivation cycle are 48h for lower progress, and OD600 > 1.0 is controlled after culture.
(2) bacterium solution cultivated is halophilic vibrio mixed microorganism microbial inoculum.
(3) microbial inoculum is used to handle the waste water that TDS is 5%, acetic acid is 1000mg/L under aerobic condition, for 24 hours, 48h,
The degradation rate of acetic acid is respectively 22.5%, 80.8%, 90.6% after 72h.
(4) microbial inoculum is used to handle TDS is 18%, acetic acid is 1200mg/L and NO3- N is the waste water of 100mg/L, control
NO after dissolved oxygen < 0.5mg/L, 72h processed3The removal rate of-N is 95.2%.
(5) microbial inoculum is used to handle TDS is 12%, glucose is 800mg/L and NO3- N is the waste water of 100mg/L, control
NO after dissolved oxygen < 0.2mg/L, 72h processed3The removal rate of-N is 76.3%.
(6) microbial inoculum is used to handle TDS is 16%, glucose is 900mg/L and NO3- N is the waste water of 100mg/L, control
NO after dissolved oxygen < 0.2mg/L, 72h processed3The removal rate of-N is 83.5%.
(7) microbial inoculum is used to handle TDS is 22%, glucose is 900mg/L and NO3- N is the waste water of 100mg/L, control
Dissolved oxygen < 0.2mg/L processed, for 24 hours, NO after 48h, 72h3The removal rate of-N is respectively 5.6%, 32.8%, 82.7%.
(8) microbial inoculum is used to handle TDS is 28%, glucose is 900mg/L and NO3- N is the waste water of 100mg/L, control
Dissolved oxygen < 0.2mg/L processed, for 24 hours, NO after 48h, 72h3The removal rate of-N is respectively 2.8%, 40.2%, 86.3%.
The complex micro organism fungicide that embodiment 11 is prepared using thermophilic salt denitrifying bacterium YL5-2
(1) medium component is glycerol 500mg/L, glucose 250mg/L, methanol 500mg/L, methylamine 200mg/L, chlorination
100~250g/L of sodium, sodium acetate 250mg/L, trisodium citrate 250mg/L, yeast powder 100mg/L, peptone 200mg/L, ox
Meat extract 200mg/L, microelement is a small amount of, and pH is 7.5~8.0.Fluid nutrient medium sterilizes, while controlling moisture evaporation.
(2) in the triangular flask of 1L simultaneously be inoculated with thermophilic salt denitrifying bacterium YL5-2, after cultivate 48h under the conditions of 35 DEG C, cultivated
The variation of the lost influence salinity to keep the skin wet in journey.Every culture 72h is once transferred, and preceding OD600 palpus of transferring every time
Not less than 1.5.
(3) 1L culture solution, which is inoculated into the aerobic fermentation tank of 20L, is cultivated, and medium component remains unchanged.Still it trains
48h is supported, cultivation temperature is 35 DEG C, mixing speed 100rpm, and dissolved oxygen is not less than 2.0mg/L.It controls in 48h post-fermentation tank
The OD600 of bacterium solution is not less than 2.5.
(4) 20L culture solution, which is inoculated into, continues to cultivate in the aerobic fermentation tank of 1000L, and condition of culture is as hereinbefore.Then
Culture scale can be amplified as needed or Batch Culture.
(5) bacterium solution for obtaining culture is carried out with using the seawater bacterium solution that enrichment culture obtains under the conditions of TDS > 10%
Mixing, it is as a kind of to contain thermophilic salt denitrifying bacterium YL5-2 complex micro organism fungicide.
(6) microbial inoculum is used to handle COD≤1000mg/L and NO3- N≤60mg/L waste water:
1. the NO after TDS is 3.5%, 72h3The removal efficiency of-N can reach 82.1%.
2. the NO after TDS is 7.5%, 72h3The removal efficiency of-N can reach 93.5%.
3. the NO after TDS is 12%, 72h3The removal efficiency of-N can reach 96.6%.
4. the NO after TDS is 18%, 72h3The removal efficiency of-N can reach 97.2%.
5. the NO after TDS is 26%, 72h3The removal efficiency of-N can reach 90.3%.
(7) composite bacteria agent is applied to the processing of preserved szechuan pickle waste water, water inlet TDS be 6%~7%, COD be 2000~
3000mg/L、NO3Under conditions of-N is 150~200mg/L, using activated sludge process A/O+ catalytic oxidation+flocculation sedimentation technique,
Continuous operation one month, COD average removal rate can reach 90% or more, NO3- N average removal rate can reach 85% or more.
(8) composite bacteria agent is applied to the restoration of the ecosystem of estuary pollution wetland.
(9) composite bacteria agent is applied to the reparation of offshore area polluted underground water, dirty by adding microbial inoculum and Hydraulic Circulation
The degradation speed of COD is obviously accelerated in dye underground water.
Sequence table
<110>Co., Ltd, Lianyungang Design and Research Institute (Lanai Engineering Co.)
<120>a kind of microbial bacterial agent and its application containing thermophilic salt denitrifying bacterium YL5-2
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 1431
<212> DNA
<213>Halovibrio belongs to (Halovibrio sp.)
<400> 1
cccatggggg cagctacaca tgcagtcgag cggcagcagc tccttcggga ggctggcgag 60
cggcggacgg gtgagtaacg catgggaact tacccagtag tgggggatag cccggggaaa 120
cccggattaa taccgcatac gccctgaggg ggaaagcggg ctccggctcg cgctattgga 180
tgggcccatg tcggattagt tagttggtgg ggtaatggcc taccaaggcg acgatccgta 240
gctggtctga gaggatgatc agccacaccg ggactgagac acggcccgga ctcctacggg 300
aggcagcagt ggggaatatt ggacaatggg ggcaaccctg atccagccat gccgcgtgtg 360
tgaagaaggc cttagggttg taaagcactt tcagcaggga ggaaaagctg atcgttaata 420
ccggtcagtg ttgacgttac ctgcagaaga agcaccggct aactccgtgc cagcagccgc 480
ggtaatacgg agggtgcaag cgttaatcgg aattactggg cgtaaagggc gcgtaggcgg 540
tttggtaagc gagttgtgaa agccccgggc tcaacctggg aatggcaatt cgaactgcca 600
agctagaatg cagcagaggg cagtggaatt ccaggtgtag cggtgaaatg cgtagatatc 660
tggaggaaca ccagtggcga aggcgactgc ctgggctgac actgacgctg aggtgcgaaa 720
gcgtgggtag caaacaggat tagataccct ggtagtccac gctgtaaacg ctgagaacta 780
gtcgttgggg ctattagagc cttagtgacg cagctaacgc gataagttct ccgcctgggg 840
agtacggccg caaggttaaa actcaaatga attgacgggg gcccgcacaa gcggtggagc 900
atgtggttta attcgacgca acgcgaagaa ccttacctgg tcttgacatc ctgcgaactt 960
ggtagagata ccttggtgcc ttcgggagcg cagtgacagg tgctgcatgg ccgtcgtcag 1020
ctcgtgtcgt gagatgttgg gttaagtccc gtaacgagcg caacccttgt ccttagttgc 1080
cagcggtccg gccgggaact ctagggagac tgccggtgac aaaccggagg aaggtgggga 1140
tgacgtcagg tcatcatggc ccttacggcc agggctacac acgtgctaca atggggcgca 1200
cagagggcag caagcgcgcg agtgcaagcg aatcccttaa aacgcctcgt agtccggatc 1260
ggagtctgca actcgactcc gtgaagtcgg aatcgctagt aatcgcagat cagaatgctg 1320
cggtgaatac gttcccgggc cttgtacaca ccgcccgtca caccatggga gtggactgca 1380
ccagaagcgg ttagtctaac cttcgggagg acgatcgcca cggtgtctgt a 1431
Claims (5)
1. a kind of microbial bacterial agent, it is characterised in that contain thermophilic salt denitrifying bacterium YL5-2 fermentation liquid, YL5-2 Halovibrio
The new species of category, are preserved in China General Microbiological culture presevation administrative center, and deposit number is CGMCC NO.16315, preservation day
Phase is on August 26th, 2018.
2. microbial bacterial agent according to claim 1, it is characterised in that the microbial bacterial agent is composite bacteria agent, is also contained
There are other wastewater treatment bacterium.
3. application of the microbial bacterial agent described in claim 1 in high-salt wastewater processing.
4. application according to claim 3, it is characterised in that microbial bacterial agent described in claim 1 salinity 5%~
Application in the biological denitrificaion processing of 25% high-salt wastewater.
5. application according to claim 3, it is characterised in that microbial bacterial agent described in claim 1 is under anoxic conditions
Or the high-salt wastewater under aerobic condition to salinity greater than 10% carries out denitrification.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109928505A (en) * | 2019-03-15 | 2019-06-25 | 江苏大学 | A method of pickling waste water is handled using salt algae, Halophiles |
| CN111040962A (en) * | 2019-11-29 | 2020-04-21 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | New strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion |
| WO2020134728A1 (en) * | 2018-12-29 | 2020-07-02 | 中蓝连海设计研究院有限公司 | Halophilic denitrifying bacteria yl5-2 and application thereof |
| CN113502247A (en) * | 2021-07-12 | 2021-10-15 | 徐韡卿 | Composite functional microbial inoculum for removing total nitrogen from high-salinity wastewater and application thereof |
| CN114249432A (en) * | 2020-09-24 | 2022-03-29 | 中蓝连海设计研究院有限公司 | Biological promoter and application thereof |
| CN114250165A (en) * | 2020-09-24 | 2022-03-29 | 中蓝连海设计研究院有限公司 | Halophilic fish bacillus LH-B.0015 and microbial inoculum and application thereof |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747014A (en) * | 2012-05-29 | 2012-10-24 | 北京盖雅技术中心有限公司 | High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment |
| CN103074286A (en) * | 2013-01-28 | 2013-05-01 | 北京大学 | High-salt heterotrophic nitrification-aerobic denitrification dephosphorization salinivibrio and application of salinivibrio in wastewater treatment |
| CN103805529A (en) * | 2012-11-14 | 2014-05-21 | 中国科学院过程工程研究所 | Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof |
| CN105886444A (en) * | 2016-06-25 | 2016-08-24 | 山东文远建材科技股份有限公司 | Compound microbial agent as well as preparation method and use method thereof |
-
2018
- 2018-12-29 CN CN201811640095.8A patent/CN109456926B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102747014A (en) * | 2012-05-29 | 2012-10-24 | 北京盖雅技术中心有限公司 | High-saline biological denitrification salinivibrio strain and application thereof in wastewater treatment |
| CN103805529A (en) * | 2012-11-14 | 2014-05-21 | 中国科学院过程工程研究所 | Halomonas campisalis with heterotrophic nitrification aerobic denitrification function and application thereof |
| CN103074286A (en) * | 2013-01-28 | 2013-05-01 | 北京大学 | High-salt heterotrophic nitrification-aerobic denitrification dephosphorization salinivibrio and application of salinivibrio in wastewater treatment |
| CN105886444A (en) * | 2016-06-25 | 2016-08-24 | 山东文远建材科技股份有限公司 | Compound microbial agent as well as preparation method and use method thereof |
Non-Patent Citations (2)
| Title |
|---|
| D. YU. SOROKIN等: "Extremely halophilic denitrifying bacteria from hypersaline inland lakes, Halovibrio denitrificans sp. nov. and Halospina denitrificans gen. nov., sp. nov., and evidence that the genus name Halovibrio Fendrich 1989 with the type species Halovibrio variabil", 《INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY》 * |
| 李健: "一株中度嗜盐菌同步异养硝化好氧反硝化脱氮性质研究", 《中国硕士学位论文全文数据库 工程科技I辑》 * |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020134728A1 (en) * | 2018-12-29 | 2020-07-02 | 中蓝连海设计研究院有限公司 | Halophilic denitrifying bacteria yl5-2 and application thereof |
| CN109928505A (en) * | 2019-03-15 | 2019-06-25 | 江苏大学 | A method of pickling waste water is handled using salt algae, Halophiles |
| CN111040962A (en) * | 2019-11-29 | 2020-04-21 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | New strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion |
| CN111040962B (en) * | 2019-11-29 | 2023-03-28 | 新疆农业科学院微生物应用研究所（中国新疆-亚美尼亚生物工程研究开发中心） | New strain marine bacterial strain XAAS-72 and application thereof in plant antibiosis and growth promotion |
| CN114249432A (en) * | 2020-09-24 | 2022-03-29 | 中蓝连海设计研究院有限公司 | Biological promoter and application thereof |
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| CN114250165B (en) * | 2020-09-24 | 2024-04-23 | 中蓝连海设计研究院有限公司 | Bacillus saliphilus LH-B.0015 and microbial inoculum and application thereof |
| CN113502247A (en) * | 2021-07-12 | 2021-10-15 | 徐韡卿 | Composite functional microbial inoculum for removing total nitrogen from high-salinity wastewater and application thereof |
| CN113502247B (en) * | 2021-07-12 | 2023-08-08 | 徐韡卿 | Composite functional microbial agent for total nitrogen removal of high-salt wastewater and application thereof |
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