CN109337832A - A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application - Google Patents
A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application Download PDFInfo
- Publication number
- CN109337832A CN109337832A CN201810209286.2A CN201810209286A CN109337832A CN 109337832 A CN109337832 A CN 109337832A CN 201810209286 A CN201810209286 A CN 201810209286A CN 109337832 A CN109337832 A CN 109337832A
- Authority
- CN
- China
- Prior art keywords
- anthropi
- nitrogen
- ammonia nitrogen
- tac
- ammonia
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
-
- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2101/00—Nature of the contaminant
- C02F2101/30—Organic compounds
- C02F2101/38—Organic compounds containing nitrogen
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biotechnology (AREA)
- Environmental & Geological Engineering (AREA)
- Hydrology & Water Resources (AREA)
- Water Supply & Treatment (AREA)
- Biodiversity & Conservation Biology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The present invention relates to a kind of anthropi TAC-2 (Ochrobacterum sp.TAC-2) of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification, deposit number is CCTCC NO:M 2018028.The bacterium can realize the process of synchronous nitration denitrification denitrogenation in aerobic environment under the conditions of high ammonia nitrogen, high salinity, effectively remove high ammonia-nitrogen wastewater, the total nitrogen in high salinity waste water, the ammonia nitrogen being particularly suitable in removal high ammonia-nitrogen wastewater, has the meaning being widely popularized.
Description
Technical field
The invention belongs to technical field of environmental microorganism, and in particular to a kind of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification
Anthropi and its application.
Background technique
In recent years, the discharge amount of high ammonia-nitrogen wastewater (200 mg/L of ammonia nitrogen concentration >) is increasing.High ammonia-nitrogen wastewater processing
Improper to be directly discharged into water body, phenomena such as will lead to water eutrophication, is on the rise, and Water quality is caused to deteriorate.In order to control water
Body ammonia and nitrogen pollution, China have promulgated stringent pollutant catabolic gene new standard, and wherein the primary standard of ammonia nitrogen is 5.0 mg/L.New mark
Effective control of the standard to ammonia nitrogen especially proposes new requirement to effective control of ammonia nitrogen in high ammonia-nitrogen wastewater.
Due to great number cost and secondary pollution problem, remove ammonia nitrogen in high ammonia-nitrogen wastewater physical chemistry method (such as blow-off method,
The ammonia still way of distillation, break point chlorination method and FENTON oxidizing process etc.) only there is application in special chemical industry.And biological denitrificaion
The advantages that pollution-free with its, economic and safe is considered as that current most promising water body denitrogenates method.
Traditional biological denitrogenation technology mainly includes A/O technique (anaerobic-aerobic Process), short-cut nitrification and denitrification technique, nitre
The techniques such as change+Anammox.These bio-denitrification technologies are all the characteristics that Autotrophic nitrification bacterium is utilized, and reach removal ammonia nitrogen
Purpose.However, since a large amount of free ammonias contained in high-concentration ammonia nitrogenous wastewater have extremely strong suppression to the nitrobacteria of autotrophic type
Production is used, and is caused Autotrophic nitrification bacterium ammoxidation process to be suppressed, is influenced NH_3-N treating effect, these biological methods is caused to locate
Treatment effect is bad when managing high ammonia-nitrogen wastewater.In addition, the two-part that these biological denitrification process do not get rid of aerobic-anaerobic combination is raw
Object denitrogenation limitation, need a large amount of space to separate the storage tank of aerobic environment and anaerobic environment so that the construction of denitrification equipment at
This is higher.
In recent years, researcher has found that special heterotrophic nitrification-aerobic denitrification bacterium, this kind of bacterium have synchronous nitre
Change and denitrifying characteristic, can realize ammonia nitrogen and total nitrogen simultaneous removing under complete aerobic condition in a reactor, solve
The contradiction of nitrification and denitrification process.With the continuous deepening of research, it was found that such bacterium has salt tolerant, low temperature resistant, resistance to
The biochemical characteristics such as high ammonia nitrogen and resistance to poor nutrient environment.
Ochrobactrum is a kind of obligate aerobic, stringent respiratory metabolism, can utilize various amino acid, organic acid and carbon aquation
The flora that object is carbon source is closed, recording Ochrobactrum there is presently no document has the function of heterotrophic nitrification-aerobic denitrification.
Summary of the invention
The object of the present invention is to provide a kind of anthropis of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification, which can
The process that synchronous nitration denitrification denitrogenation is realized in aerobic environment under the conditions of high ammonia nitrogen, high salinity, effectively removes high ammonia nitrogen
Total nitrogen in waste water, high salinity waste water, the ammonia nitrogen being particularly suitable in removal high ammonia-nitrogen wastewater, has the meaning being widely popularized.
Total nitrogen (TN) of the present invention, refers to ammonia nitrogen (NH4 +-N\ NH3- N), nitrate nitrogen (NO3 -), nitrite nitrogen
(NO2 -) summation.
The technical scheme is that
The anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification, deposit number be CCTCC NO:M 2018028(in
On January 12nd, 2018 is preserved in China typical culture collection center, address: Wuhan, China Wuhan University), classification naming are as follows:OchrobacterumSp. TAC-2,16S rDNA sequence are as shown in SEQ ID NO:1.
The anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification of the present invention, belongs to Ochrobactrum.Bacterium colony
1 mm of diameter or so, translucent, protrusion, neat in edge, surface is glossy, and color is white transparent, is Gram-negative.
Optimum growth temp is 25~40 DEG C, and pH is 6.5~7.5.
Anthropi TAC-2 of the present invention is strong to high ammonia nitrogen, high salinity tolerance, using organic matter as electronics by
Body, NH4 +For electron donor, by NH4 +It is oxidized to NO2 -Or NO3 -；It can be supplied in aerobic environment by electronics of organic matter simultaneously
Body, NO2 -Or NO3 -For electron acceptor, by NO2 -Or NO3 -It is reduced to nitrogen.Anthropi TAC-2 of the present invention can be applied to
Processing is efficiently denitrogenated in high ammonia-nitrogen wastewater, high salinity waste water.
Anthropi of the present invention is named as anthropi in the applicationOchrobacterumSp. TAC-2 is
Isolated from the biogas slurry of pig farm, the sequencing commission Dalian treasured biotech firm of bacterial strain DNA completes, and 16S rDNA sequence is such as
Shown in SEQ ID NO:1.By a series of Physiology and biochemistries and the process optimization that carry out to the bacterial strain, experiments have shown that, the present invention is mentioned
The anthropi of confessionOchrobacterumSp. TAC-2 is completely removed high ammonia-nitrogen wastewater, the total nitrogen in high salinity waste water,
Major advantage is as follows:
(1) anthropiOchrobacterumSp. TAC-2 can be using various amino acid, organic acid and carbohydrate as carbon source
It is only nitrogen source growth with ammonia nitrogen, overcomes that traditional nitrobacteria slow growth, the generation cycle is long, biomass concentration is lower, ring
The disadvantages of border bad adaptability.
(2) anthropiOchrobacterumSp. TAC-2 can remove total nitrogen (ammonia nitrogen (NH in complete aerobic environment4 +), nitrate nitrogen (NO3 -), nitrite nitrogen (NO2 -), the two-part biology for solving the combination of traditional biological denitrification process aerobic-anaerobic is de-
Nitrogen limitation.
(3) anthropiOchrobacterumSp. TAC-2 has high ammonia nitrogen, high salinity tolerance, can efficiently remove
Total nitrogen in high ammonia-nitrogen wastewater, high salinity waste water.
(4) anthropiOchrobacterumSp. medium component needed for TAC-2 expands culture is simple, cost
It is low.It, can be with wide popularization and application especially suitable for the biological denitrificaion of high ammonia-nitrogen wastewater.
The present invention solves poor processing effect, complex process, at high cost etc. in existing high ammonia-nitrogen wastewater bio-denitrification technology
Problem.
For above and other objects of the present invention, feature and advantage can be clearer and more comprehensible, lower special act preferred embodiment, and
Cooperate attached drawing, is described in detail below.
Detailed description of the invention
Fig. 1 is anthropiOchrobacterumSp. TAC-2 is when ammonia nitrogen concentration is 400 mg/L to ammonia nitrogen and total
The removal curve of nitrogen；
Fig. 2 is anthropiOchrobacterumSp. removal curve of the TAC-2 to various concentration ammonia nitrogen.
Fig. 3 is anthropiOchrobacterumSp. TAC-2 is under different salinity to the removal curve of ammonia nitrogen.
Specific embodiment
The present invention will be further described with reference to the examples below, and embodiments of the present invention are not limited thereto.
1. experimental material
The composition of anthropi culture medium is as follows: (NH4)2SO42 g/L, Na3C6H5O710.64 g/L, Vickers salting liquid 50
ML/L, wherein Vickers salting liquid (g/L): K2HPO45.0, MgSO4 7H2O 2.5, NaCl 2.5, FeSO4 7H2O 0.05,
MnSO4 4H2O 0.05.Anthropi Medium's PH Value is adjusted to 7.0.
For carbon source other than sodium acetate, anthropi can also directly utilize the common carbon such as glucose, sucrose, sodium citrate
Source.
Remaining reagent is commercially available analysis net product.
Pig farm biogas slurry sample is derived from Chongqing wood elk Pig breeding plant (the Chongqing City Banan District town the Jiang Jia village Cai Jiasi).
2. strain enrichment is tested with optimization
1) enrichment culture: 2 pig farm mL biogas slurries is taken to be inoculated in the 250 mL triangles equipped with the broth bouillon after 100 mL sterilizing
It in bottle, sufficiently shakes up, 30 DEG C, cultivate under the conditions of 150 rmin-1,4 DEG C of refrigerators save after 2 d.It then is 2% by inoculum concentration,
Enriched microorganism is inoculated in the 250 mL triangular flasks equipped with the anthropi culture medium after 100 mL sterilizing.Above-mentioned identical item
Secondary culture under part, altogether enrichment passage 5 times.
2) pure bacterium separation: 10 times are carried out to above-mentioned enrichment bacterium solution with the sterile distilled water cooled down and is serially diluted, is made dilute
Degree of releasing is 10-1、10-2、10-3、10-4、10-5、10-6、10-7Dilution.The culture of anthropi culture medium is carried out using tilt-pour process.
Plate is placed in biochemical cultivation case, 30 DEG C of culture 2d.The good strain of growing way is repeatedly passed on, is purified.
3) optimization experiment: preparing certain density bacteria suspension using pure bacterial strain, and bacterium solution is gone out by 2% inoculation equipped with 100 mL
It in 250 mL conical flasks of the anthropi culture medium after bacterium, sufficiently shakes up, 30 DEG C, cultivate under the conditions of 150 rmin-1.If
It sets ammonia nitrogen concentration and is set to 100 mg/L, 200 mg/L, 400 mg/L, 600 mg/L, 800 mg/L, 1000 mg/L；Salinity
It is set to: 1%, 2%, 3%, 4%, 5%；Only change single factors, certain interval of time detects bacterial concentration and ammonia nitrogen concentration.
3. strain idenfication is tested
PCR amplification target fragment is carried out using QIAquick Genomic DNA Buffer Set.5 μ l are taken to carry out 3% agarose
Gel electrophoresis carries out DNA sequencing using gel extraction target fragment.The sequencing commission Dalian treasured biotech firm of DNA completes.With
Seq Forward, Seq Reverse, Seq Internal are that primer carries out DNA sequencing.16S rRNA amplification uses wide spectrum
Its sequence of primers F 27 (- AGAGTTTGATCATGGCTCAG) as described in SEQ ID NO:2 and R1492 (-
TACGGTTACCTTGTTACGACTT), sequence is as described in SEQ ID NO:3.
4. detection method
The OD value of bacterium solution is detected using UV2000 spectrophotometer, and wavelength is 600 nm.The method for monitoring and analyzing of each pollutant is joined
Examine " water and effluent monitoring analysis method " (fourth edition, China Environmental Science Press, 2002).
1 anthropi of embodimentOchrobacterumSp. ammonia nitrogen of the TAC-2 when ammonia nitrogen concentration is 400 mg/L
With total nitrogen removal ability measurement experiment
The anthropi culture medium that ammonia nitrogen concentration is 400 mg/L is configured, 100 mL anthropi cultures is taken to be based on 250 mL tapers
Bottle in, by it under the conditions of 121 DEG C 30 min of high-temp steam sterilizing, 2 mL are added into bottle with micropipettor after cooling
Anthropi bacterium solution (OD600 nm It for 1-2), is sealed using sealed membrane, is put into shaking table and is set in 30 DEG C, 150 rmin-1Condition
Lower culture, then every the OD of 24 h measurement bacterium solution600 nmBe worth and determine thalli growth situation, at the same measure in culture medium ammonia nitrogen and
The content of total nitrogen determines the removal effect of ammonia nitrogen and total nitrogen.As shown in Figure 1, anthropiOchrobacterum sp. TAC-2
Completely remove 400 mg/L ammonia nitrogens, 90% or more nitrogen removal rate.
2 anthropi of embodimentOchrobacterumSp. TAC-2 is to various concentration ammonia nitrogen removal effect experiment
It is 100 mg/L, 200 mg/L, 400 mg/L, 600 mg/L, 800 mg/L, 1000 mg/L that ammonia nitrogen concentration, which is respectively configured,
Anthropi culture medium, take respectively 100 mL difference ammonia nitrogen concentrations anthropi culture be based on 250 mL conical flasks in, will
Its 30 min of high-temp steam sterilizing under the conditions of 121 DEG C, is added 2 mL anthropis with micropipettor into bottle after cooling
Bacterium solution (OD600 nm It for 1-2), is sealed using sealed membrane, is put into shaking table and is set in 30 DEG C, 150 rmin-1Under the conditions of cultivate,
Then every the OD of 24 h measurement bacterium solution600 nmIt is worth and determines thalli growth situation, while measuring the content of ammonia nitrogen in culture medium, really
Determine the removal effect of ammonia nitrogen.As shown in Figure 2, when ammonia nitrogen concentration is less than 600 mg/L, ammonia nitrogen removal frank 100%, anthropiOchrobacterumSp. TAC-2 maximum ammonia nitrogen tolerable concentration is up to 800 mg/L, and ammonia nitrogen removal frank 34.2%, this is main
It is to inhibit the activity of bacterial strain due to containing a large amount of free ammonia in ammonia nitrogen in high density culture medium.
3 anthropi of embodimentOchrobacterumSp. TAC-2 high ammonia nitrogen removal effect under the conditions of different salinity
Fruit is tested
The anthropi culture medium that salinity is 1%, 2%, 3%, 4%, 5% is respectively configured, the ammonia nitrogen concentration of each anthropi culture medium is equal
For 400 mg/L, the anthropi culture of 100 mL different salinities is taken to be based in 250 mL conical flasks respectively, by it at 121 DEG C
Under the conditions of 30 min of high-temp steam sterilizing, 2 mL anthropi bacterium solutions are added into bottle with micropipettor after cooling
(OD600 nm It for 1-2), is sealed using sealed membrane, is put into shaking table and is set in 30 DEG C, 150 rmin-1Under the conditions of cultivate, then
Every the OD of 24 h measurement bacterium solution600 nmIt is worth and determines thalli growth situation, while measuring the content of ammonia nitrogen in culture medium, determines ammonia
The removal effect of nitrogen.From the figure 3, it may be seen that when salinity is less than 2%, ammonia nitrogen removal frank 85.7%, anthropiOchrobacterum
Sp. TAC-2 maximum salinity tolerable concentration is up to 2%, ammonia nitrogen removal frank 33.8%, the reason is that since high salinity medium seeps
Higher, microbial cell plasmolysis is pressed thoroughly, is hindered its growth and is even stopped.
The anthropi TAC-2 that the present invention is screened from pig farm biogas slurry is strong to high ammonia nitrogen, high salinity tolerance, and
And can use organic carbon source is sole carbon source, ammonia nitrogen is that only nitrogen source carries out metabolism, passes through the anti-nitre of heterotrophic nitrification-aerobic
Change effect completely removes high ammonia nitrogen, while realizing that total nitrogen removes.The bacterial strain also can be unique with nitrate nitrogen, nitrite nitrogen
Nitrogen source is efficiently removed it by aerobic denitrification.The bacterial strain is applied to the processing of high ammonia-nitrogen wastewater, high salinity waste water,
The synchronous removal that ammonia nitrogen under single aerobic condition, total nitrogen can be achieved, helps to solve the problems, such as biological denitrificaion under high ammonia-nitrogen condition,
It has a extensive future.
Listed above is only three specific embodiments of the invention.It is clear that the invention is not restricted to which above embodiments, may be used also
With there are many deformations.All changes that those skilled in the art directly can export or associate from present disclosure
Shape is considered as protection scope of the present invention.
Sequence table
<110>Chongqing University of Technology
<120>a kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 425
<212> DNA
<213>anthropi (the 16S rDNA sequence of Ochrobacterum sp. TAC-2)
<400> 1
gtggggaata ttgcacaatg ggggaaaccc tgatgcagca acgccgcgtg agtgatgacg 60
gtcttcggat tgtaaagctc tgtctttggg gacgataatg acggtaccca aggaggaagc 120
cacggctaac tacgtgccag cagccgcggt aatacgtagg tggcgagcgt tgtccggatt 180
tactgggcgt aaagggagcg taggcggatt cttaagtggg atgtgaaata cctgggctta 240
acctgggtgc tgcattccaa actgggaatc tagagtgcag gaggggagag tggaattcct 300
agtgtagcgg tgaaatgcgt agagattagg aagaacacca gtggcgaagg cgactctctg 360
gactgtaact gacgctgagg ctcgaaagcg tggggagcaa acaggattag aaacccctgt 420
agtcc 425
<210> 2
<211> 20
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 2
agagtttgat catggctcag 20
<210> 3
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<400> 3
tacggttacc ttgttacgac tt 22
Claims (7)
1. a kind of anthropi TAC-2 of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification, which is characterized in that its deposit number is
CCTCC NO:M 2018028。
2. anthropi TAC-2 according to claim 1, which is characterized in that the anthropi TAC-2 belongs to pale bar
Pseudomonas.
3. anthropi TAC-2 according to claim 1, which is characterized in that the 16S rDNA of the anthropi TAC-2
Sequence is as shown in SEQ ID NO:1.
4. the application of any anthropi TAC-2 of claim 1-3 total nitrogen in removal high ammonia-nitrogen wastewater.
5. application according to claim 4, which is characterized in that concentration≤800mg/L of ammonia nitrogen in the high ammonia-nitrogen wastewater.
6. the application of any anthropi TAC-2 of claim 1-3 total nitrogen in removal high salinity waste water.
7. any anthropi TAC-2 of claim 1-3 is preparing the application in biocatalyst.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810209286.2A CN109337832B (en) | 2018-03-14 | 2018-03-14 | High-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification ochrobactrum and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810209286.2A CN109337832B (en) | 2018-03-14 | 2018-03-14 | High-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification ochrobactrum and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN109337832A true CN109337832A (en) | 2019-02-15 |
| CN109337832B CN109337832B (en) | 2021-06-15 |
Family
ID=65291362
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810209286.2A Active CN109337832B (en) | 2018-03-14 | 2018-03-14 | High-ammonia-nitrogen-resistant heterotrophic nitrification-aerobic denitrification ochrobactrum and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109337832B (en) |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082387A (en) * | 2018-03-14 | 2018-12-25 | 重庆理工大学 | It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen |
| CN111233148A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Efficient biomembrane synchronous nitrification and denitrification low-carbon sewage denitrification process |
| CN113388553A (en) * | 2021-07-28 | 2021-09-14 | 重庆理工大学 | Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof |
| CN113416681A (en) * | 2021-07-28 | 2021-09-21 | 苏州氮缘生态科技有限公司 | Low-carbon and high-nitrogen resistant heterotrophic nitrification-aerobic denitrification bacterium and application thereof |
| CN115975891A (en) * | 2023-02-28 | 2023-04-18 | 南京理工大学 | Ochrobactrum capable of degrading N-methylpyrrolidone and application of Ochrobactrum capable of degrading N-methylpyrrolidone in wastewater treatment |
| CN116103188A (en) * | 2022-07-28 | 2023-05-12 | 新疆河润科技有限公司 | Pallidum HRKJ-3, microbial preparation and application thereof |
Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1513946B1 (en) * | 2002-06-14 | 2007-05-16 | DSM IP Assets B.V. | Polypeptides having alpha-h-alpha amino acid amide racemase activity and nucleic acids encoding the same |
| WO2016075082A1 (en) * | 2014-11-10 | 2016-05-19 | Sandoz Ag | Stereoselective reductive amination of alpha-chiral aldehydes using omega-transaminases for the synthesis of precursors of pregabalin and brivaracetam |
| CN107058150A (en) * | 2016-11-18 | 2017-08-18 | 暨南大学 | One plant of human pallid bacillus FX02 bacterial strain and its application in denitrogenation of waste water |
| CN108585195A (en) * | 2018-03-14 | 2018-09-28 | 重庆理工大学 | Low temperature low-carbon ammonia nitrogen wastewater treatment device and method based on solid phase denitrification and heterotrophic nitrification-aerobic denitrification |
| CN108949611A (en) * | 2018-06-08 | 2018-12-07 | 暨南大学 | One plant of Dell Ford DNF-02 and its application in denitrogenation of waste water |
| CN109082387A (en) * | 2018-03-14 | 2018-12-25 | 重庆理工大学 | It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen |
| CN109368938A (en) * | 2018-12-04 | 2019-02-22 | 重庆士继生态环境科技有限公司 | A kind of full aerobic nitrogen removal technique of livestock breeding wastewater pre-treatment and integration apparatus |
| CN109734198A (en) * | 2019-01-31 | 2019-05-10 | 重庆理工大学 | A kind of the dioxygen layer biofilm reactor and wastewater treatment method of wastewater treatment |
| CN110387339A (en) * | 2019-07-03 | 2019-10-29 | 中国科学院微生物研究所 | One plant of intermediate anthropi B522 and its application |
| CN110804568A (en) * | 2019-11-14 | 2020-02-18 | 重庆理工大学 | Complex microbial inoculant and application thereof |
| CN111233148A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Efficient biomembrane synchronous nitrification and denitrification low-carbon sewage denitrification process |
| CN111233166A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Method for biofilm formation of biofilm reactor by using microbial inoculum |
| CN111422976A (en) * | 2020-03-09 | 2020-07-17 | 重庆理工大学 | Process for treating high ammonia nitrogen wastewater by using moving bed biofilm reactor |
| CN111484204A (en) * | 2020-05-07 | 2020-08-04 | 重庆理工大学 | Combined process and treatment system for treating high ammonia nitrogen wastewater |
-
2018
- 2018-03-14 CN CN201810209286.2A patent/CN109337832B/en active Active
Patent Citations (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP1513946B1 (en) * | 2002-06-14 | 2007-05-16 | DSM IP Assets B.V. | Polypeptides having alpha-h-alpha amino acid amide racemase activity and nucleic acids encoding the same |
| WO2016075082A1 (en) * | 2014-11-10 | 2016-05-19 | Sandoz Ag | Stereoselective reductive amination of alpha-chiral aldehydes using omega-transaminases for the synthesis of precursors of pregabalin and brivaracetam |
| CN107058150A (en) * | 2016-11-18 | 2017-08-18 | 暨南大学 | One plant of human pallid bacillus FX02 bacterial strain and its application in denitrogenation of waste water |
| CN108585195A (en) * | 2018-03-14 | 2018-09-28 | 重庆理工大学 | Low temperature low-carbon ammonia nitrogen wastewater treatment device and method based on solid phase denitrification and heterotrophic nitrification-aerobic denitrification |
| CN109082387A (en) * | 2018-03-14 | 2018-12-25 | 重庆理工大学 | It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen |
| CN108949611A (en) * | 2018-06-08 | 2018-12-07 | 暨南大学 | One plant of Dell Ford DNF-02 and its application in denitrogenation of waste water |
| CN109368938A (en) * | 2018-12-04 | 2019-02-22 | 重庆士继生态环境科技有限公司 | A kind of full aerobic nitrogen removal technique of livestock breeding wastewater pre-treatment and integration apparatus |
| CN109734198A (en) * | 2019-01-31 | 2019-05-10 | 重庆理工大学 | A kind of the dioxygen layer biofilm reactor and wastewater treatment method of wastewater treatment |
| CN110387339A (en) * | 2019-07-03 | 2019-10-29 | 中国科学院微生物研究所 | One plant of intermediate anthropi B522 and its application |
| CN110804568A (en) * | 2019-11-14 | 2020-02-18 | 重庆理工大学 | Complex microbial inoculant and application thereof |
| CN111233148A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Efficient biomembrane synchronous nitrification and denitrification low-carbon sewage denitrification process |
| CN111233166A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Method for biofilm formation of biofilm reactor by using microbial inoculum |
| CN111422976A (en) * | 2020-03-09 | 2020-07-17 | 重庆理工大学 | Process for treating high ammonia nitrogen wastewater by using moving bed biofilm reactor |
| CN111484204A (en) * | 2020-05-07 | 2020-08-04 | 重庆理工大学 | Combined process and treatment system for treating high ammonia nitrogen wastewater |
Non-Patent Citations (8)
| Title |
|---|
| SHAO, SICHENG等: "Degradation of oxytetracycline (OTC) and nitrogen conversion characteristics using a novel strain", 《CHEMICAL ENGINEERING JOURNAL》 * |
| XIAO HUANG等: "Characteristics of two novel cold- and salt-tolerant ammonia-oxidizing bacteria from Liaohe Estuarine Wetland", 《MARINE POLLUTION BULLETIN》 * |
| XIN LEI等: "Simultaneous nitrification and denitrification without nitrite accumulation by a novel isolated Ochrobactrum anthropic LJ81", 《BIORESOURCE TECHNOLOGY》 * |
| 李紫惠: "异养硝化-好氧反硝化菌XH02的脱氮特性及强化脱氮过程中菌群结构变化的研究", 《万方学位论文数据库》 * |
| 李紫惠等: "响应面优化好氧反硝化菌的硝化影响因子", 《环境科学与技术》 * |
| 陈君: "固定化亚硝酸菌反硝化菌协同除氨氮废水及生物膜观察", 《万方学位论文数据库》 * |
| 黄婧等: "1株氨态氮降解菌JN-2的分离、筛选和分子鉴定 ", 《福建农业学报》 * |
| 黄婧等: "畜禽养殖污水中高效氨氮降解菌的筛选、鉴定及生长条件研究 ", 《环境工程学报》 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109082387A (en) * | 2018-03-14 | 2018-12-25 | 重庆理工大学 | It is a kind of can low temperature remove heterotrophic nitrification-aerobic denitrification composite bacteria agent and its application of high ammonia nitrogen |
| CN111233148A (en) * | 2020-01-19 | 2020-06-05 | 重庆理工大学 | Efficient biomembrane synchronous nitrification and denitrification low-carbon sewage denitrification process |
| CN113388553A (en) * | 2021-07-28 | 2021-09-14 | 重庆理工大学 | Ammonia nitrogen resistant composite microbial inoculum and application and preparation method thereof |
| CN113416681A (en) * | 2021-07-28 | 2021-09-21 | 苏州氮缘生态科技有限公司 | Low-carbon and high-nitrogen resistant heterotrophic nitrification-aerobic denitrification bacterium and application thereof |
| CN116103188A (en) * | 2022-07-28 | 2023-05-12 | 新疆河润科技有限公司 | Pallidum HRKJ-3, microbial preparation and application thereof |
| CN115975891A (en) * | 2023-02-28 | 2023-04-18 | 南京理工大学 | Ochrobactrum capable of degrading N-methylpyrrolidone and application of Ochrobactrum capable of degrading N-methylpyrrolidone in wastewater treatment |
Also Published As
| Publication number | Publication date |
|---|---|
| CN109337832B (en) | 2021-06-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN109337832A (en) | A kind of anthropi of resistance to high ammonia nitrogen heterotrophic nitrification-aerobic denitrification and its application | |
| CN103756947B (en) | The preparation method of a kind of acinetobacter calcoaceticus and microbial inoculum thereof | |
| CN110655198B (en) | Method for treating nitrogen-containing wastewater by using heterotrophic nitrification-aerobic denitrification paracoccus strain | |
| JP4631082B2 (en) | Nitrification and denitrification method that simultaneously removes NH4 + and NO3- using microorganisms | |
| CN104862260B (en) | One plant of arthrobacterium and its application with aerobic denitrification ability | |
| CN109576187A (en) | One plant of cyanide degradation bacterial strain and the method for utilizing the strains for degrading cyanide | |
| CN114703095B (en) | Pseudomonas adulthood and application thereof in field of sewage and wastewater purification | |
| CN105420165A (en) | Aerobic denitrifying bacteria and applications therefor | |
| CN110656057B (en) | Heterotrophic nitrification-aerobic denitrification paracoccus strain, seed liquid, preparation method and application thereof | |
| CN110283739A (en) | The denitrifying bacteria of one plant of salt tolerant and its application | |
| CN104611279B (en) | A kind of red city Rhodococcus sp LH N13 and its microbial bacterial agent and purposes | |
| CN111088200B (en) | Heterotrophic nitrification aerobic denitrifying bacterium and application thereof in pig-raising wastewater treatment | |
| CN113234626A (en) | Strain with heterotrophic nitrification-aerobic denitrification function and application thereof | |
| CN115386520B (en) | Rhodococcus pyridine-philic RL-GZ01 strain and application thereof | |
| CN113151063B (en) | Citrobacter freundii AS11 and application thereof in sewage treatment | |
| CN106591181B (en) | A kind of Mysore arthrobacterium and its application in purifying sea water cultivation nitrogenous effluent | |
| CN110184217B (en) | Salt-tolerant denitrifying bacterium taking nitrite as nitrogen source and application thereof | |
| CN115820466B (en) | Sulfur autotrophic denitrification strain, bacterial preparation and application thereof | |
| CN114292798B (en) | Anaerobic denitrifying strain and application thereof in riverway water body remediation | |
| CN113215027B (en) | Alcaligenes aquaticum AS1 and application thereof in sewage treatment | |
| CN113583897B (en) | Bacillus aryabhattai FL05 and application thereof | |
| CN109593673B (en) | Flavobacterium JX-1 and application thereof in sewage treatment | |
| CN113699060A (en) | Low-carbon and high-nitrogen resistant heterotrophic nitrification-aerobic denitrification sphingosine box bacterium and application thereof | |
| CN101811780A (en) | Preparation method and application of halophilic decontamination bacterial agent | |
| CN113005063B (en) | Pseudomonas putida GY13 and application thereof in sewage treatment |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |