CN109090117B - Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof - Google Patents
Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof Download PDFInfo
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- CN109090117B CN109090117B CN201810999000.5A CN201810999000A CN109090117B CN 109090117 B CN109090117 B CN 109090117B CN 201810999000 A CN201810999000 A CN 201810999000A CN 109090117 B CN109090117 B CN 109090117B
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- ethylicin
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- KPZYYKDXZKFBQU-UHFFFAOYSA-N phenazine-1-carboxamide Chemical compound C1=CC=C2N=C3C(C(=O)N)=CC=CC3=NC2=C1 KPZYYKDXZKFBQU-UHFFFAOYSA-N 0.000 title claims abstract description 85
- OICGFSUWXCJLCO-UHFFFAOYSA-N 1-ethylsulfonylsulfanylethane Chemical compound CCSS(=O)(=O)CC OICGFSUWXCJLCO-UHFFFAOYSA-N 0.000 title claims abstract description 61
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 20
- 239000003899 bactericide agent Substances 0.000 title claims abstract description 19
- 150000001875 compounds Chemical class 0.000 title claims abstract description 17
- 241000234435 Lilium Species 0.000 claims abstract description 39
- 239000003795 chemical substances by application Substances 0.000 abstract description 25
- 230000000694 effects Effects 0.000 abstract description 18
- 238000012360 testing method Methods 0.000 abstract description 16
- 231100000419 toxicity Toxicity 0.000 abstract description 14
- 230000001988 toxicity Effects 0.000 abstract description 14
- 239000000575 pesticide Substances 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 8
- 238000000034 method Methods 0.000 abstract description 7
- 238000000338 in vitro Methods 0.000 abstract description 6
- 241000703939 Gracilariopsis longissima Species 0.000 abstract description 3
- 230000012010 growth Effects 0.000 abstract description 3
- 239000003814 drug Substances 0.000 description 14
- 238000011282 treatment Methods 0.000 description 11
- 230000005764 inhibitory process Effects 0.000 description 9
- 230000001018 virulence Effects 0.000 description 8
- 241000123650 Botrytis cinerea Species 0.000 description 7
- 238000013329 compounding Methods 0.000 description 7
- 201000010099 disease Diseases 0.000 description 7
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 239000001963 growth medium Substances 0.000 description 5
- 230000007246 mechanism Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 206010059866 Drug resistance Diseases 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 4
- 244000052616 bacterial pathogen Species 0.000 description 4
- -1 phenazine compound Chemical class 0.000 description 4
- QXJKBPAVAHBARF-BETUJISGSA-N procymidone Chemical compound O=C([C@]1(C)C[C@@]1(C1=O)C)N1C1=CC(Cl)=CC(Cl)=C1 QXJKBPAVAHBARF-BETUJISGSA-N 0.000 description 4
- 240000008058 Lilium brownii Species 0.000 description 3
- 235000015982 Lilium brownii Nutrition 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 2
- 241001465180 Botrytis Species 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 241001052560 Thallis Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 230000003385 bacteriostatic effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 230000035784 germination Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 239000011664 nicotinic acid Substances 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 239000008223 sterile water Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 244000307697 Agrimonia eupatoria Species 0.000 description 1
- 239000005759 Diethofencarb Substances 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 102000003960 Ligases Human genes 0.000 description 1
- 108090000364 Ligases Proteins 0.000 description 1
- 241000234280 Liliaceae Species 0.000 description 1
- 235000015979 Lilium brownii var viridulum Nutrition 0.000 description 1
- 244000039138 Lilium brownii var. viridulum Species 0.000 description 1
- 241000246116 Lotus maritimus Species 0.000 description 1
- 208000031888 Mycoses Diseases 0.000 description 1
- PCNDJXKNXGMECE-UHFFFAOYSA-N Phenazine Natural products C1=CC=CC2=NC3=CC=CC=C3N=C21 PCNDJXKNXGMECE-UHFFFAOYSA-N 0.000 description 1
- 239000005828 Pyrimethanil Substances 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 230000008485 antagonism Effects 0.000 description 1
- 230000000843 anti-fungal effect Effects 0.000 description 1
- 239000012752 auxiliary agent Substances 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 235000000125 common agrimony Nutrition 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- LNJNFVJKDJYTEU-UHFFFAOYSA-N diethofencarb Chemical compound CCOC1=CC=C(NC(=O)OC(C)C)C=C1OCC LNJNFVJKDJYTEU-UHFFFAOYSA-N 0.000 description 1
- 239000004495 emulsifiable concentrate Substances 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 230000000855 fungicidal effect Effects 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 239000002917 insecticide Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 230000002438 mitochondrial effect Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000010413 mother solution Substances 0.000 description 1
- 239000003090 pesticide formulation Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 1
- 229920000053 polysorbate 80 Polymers 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- ZLIBICFPKPWGIZ-UHFFFAOYSA-N pyrimethanil Chemical compound CC1=CC(C)=NC(NC=2C=CC=CC=2)=N1 ZLIBICFPKPWGIZ-UHFFFAOYSA-N 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 238000002723 toxicity assay Methods 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/48—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with two nitrogen atoms as the only ring hetero atoms
- A01N43/60—1,4-Diazines; Hydrogenated 1,4-diazines
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
- A01N41/04—Sulfonic acids; Derivatives thereof
- A01N41/08—Sulfonic acid halides; alpha-Hydroxy-sulfonic acids; Amino-sulfonic acids; Thiosulfonic acids; Derivatives thereof
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- Life Sciences & Earth Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Dentistry (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the field of pesticide compoundingMainly relates to a compound bactericide containing phenazine-1-formamide and ethylicin and application thereof. The invention adopts a growth rate method to test the toxicity of the single agents of the phenazine-1-formamide and the ethylicin and the compound agent thereof, and simultaneously evaluates the synergistic effect of the compound agent according to a co-toxicity coefficient method. The results show that: phenazine-1-formamide and ethylicin have antibacterial activity on Gracilaria verrucosa, and EC thereof5021.0859 μ g/mL and 138.9683 μ g/mL, respectively. The two are compounded, when the weight ratio of the phenazine-1-formamide to the ethylicin is 1:1, the co-toxicity coefficient is 172 which is more than 120, and the synergistic effect is obvious. The in vitro leaf control effect test result shows that: the compound of phenazine-1-formamide and ethylicin in a ratio of 1:1 has the control effect of 83.85% on gray mold of the longya lily, and has the potential of further developing new pesticides.
Description
Technical Field
The invention belongs to the field of pesticide compounding, and particularly relates to a compound bactericide containing phenazine-1-formamide and ethylicin, and simultaneously relates to application of the bactericide in preventing and treating gray mold of lily of the Longya.
Background
Lilium brownii (Lilium brownii var. viridulum) belongs to the genus Lilium of the family liliaceae and is a perennial herb. The bulb pieces of the longya lily are similar to the dragon teeth, so the dragon teeth lily is called as the dragon teeth lily. The Longya lily contains rich trace elements beneficial to human bodies, has low content of soluble harmful heavy metals and the like, and is a green health-care food integrating food and medicines. The growth of the longya lily is often interfered by various factors, such as: temperature, humidity, fertilizer, illumination, diseases, pests, weeds and the like, wherein the gray mold of the longya lily (the gray mold of the lily for short) is an important factor influencing the yield and the quality of the longya lily. The disease is mainly a fungal disease caused by botrytis cinerea, and is very difficult to prevent and treat due to the characteristics of wide host range, easy variation, strong adaptability, quick propagation and the like. In order to effectively control the occurrence and harm of the disease in production, agricultural control, biological control and chemical control are commonly adopted, wherein the chemical control is a main means. At present, the commonly used chemical pesticides mainly comprise procymidone, pyrimethanil, diethofencarb and the like, but the long-term use of the chemical pesticides in large quantity has the risk of drug resistance of pathogenic bacteria. The results of the previous study showed that: botrytis cinerea has developed resistance to various chemical agents such as procymidone, and therefore, the search for new methods for preventing and treating gray mold is urgent.
The compounding of the two medicaments with different action mechanisms can not only prolong the service life of the medicaments, but also delay the drug resistance of pathogenic bacteria, and is an important method for solving the problem of drug resistance of pathogenic bacteria. The phenazine-1-formamide is a natural phenazine compound and has broad-spectrum antifungal activity, and the research results of the subject groups show that the action mechanism of the phenazine-1-formamide achieves the aim of controlling hyphae mainly by inhibiting the activity of cell wall synthetase and mitochondrial complex enzyme. Ethylicin is a plant bionic pesticide, the content of a general preparation is 80 percent, the ethylicin is a high-efficiency pollution-free broad-spectrum bionic bactericide, has the plant growth regulation function, can promote germination, improve the germination rate, increase the yield and improve the quality, and the action mechanism of the ethylicin is to act with substances containing sulfur radicals in thalli, so that the normal metabolism of the thalli is inhibited. Because the action mechanisms of the two single agents are different and the report of compounding the two single agents is not available, the invention determines the synergistic action and the optimal formula by measuring the indoor toxicity of the two single agents and the compound thereof on the gray mold of the lily of longya, and further determines the synergistic potential through an in vitro control effect test, aiming at selecting a proper bactericide as a bedding for effectively preventing and treating the gray mold of the lily of longya and being beneficial to better guiding the agricultural production.
Disclosure of Invention
The invention aims to solve the technical problem of providing a compound bactericide containing phenazine-1-formamide and ethylicin and simultaneously providing an application of the compound bactericide in preventing and treating gray mold of lily of the Longya.
In order to solve the technical problems, the technical scheme adopted by the invention is as follows: a compound bactericide containing phenazine-1-formamide and ethylicin contains phenazine-1-formamide and ethylicin, wherein the weight ratio of phenazine-1-formamide to ethylicin is 1: 1.
The bactericide can be prepared into a conventional pesticide formulation together with the conventional auxiliary agents (other auxiliary components acceptable in the preparation of pesticides).
The invention also provides application of the compound bactericide containing phenazine-1-formamide and ethylicin in preventing and treating gray mold of longya lily.
Phenazine-1-formamide and ethylicin have bacteriostatic activity on Gray mould of Lilium Longdentiger, and their EC5021.0859 μ g/mL and 138.9683 μ g/mL, respectively. The two are compounded, when the ratio of the phenazine-1-formamide to the ethylicin is 1:1, the co-toxicity coefficient is 172 which is more than 120, and the synergistic effect is obvious. The in vitro leaf control effect test result shows that: the compound of the phenazine-1-formamide and the ethylicin according to the proportion has the control effect of 83.85 percent on the gray mold of the lily of the dragon's teeth, which is 1.51 times and 1.48 times of the single use of the phenazine-1-formamide and the ethylicin respectively.
Compared with the prior art, the invention has the advantages that:
1. the 1:1 compounding of the phenazine-1-formamide and the ethylicin has stronger toxicity to the gray mold of the lily of the dragon tooth, the cotoxicity coefficient is 172 and is more than 120, and the synergistic effect is obvious.
2. The two agents with different action mechanisms of the phenazine-1-formamide and the ethylicin are compounded, so that the service life of the agent can be prolonged, and the drug resistance of germs is not easy to generate.
3. The invention provides a compound bactericide for preventing and treating lily gray mold, and enriches new pesticides for preventing and treating lily gray mold.
Detailed Description
The technical scheme of the invention is further explained by combining specific tests and examples, wherein the percentages in the examples are mass percentages.
Example 1 synergistic Effect of compounding phenazine-1-carboxamide and ethylicin in controlling Gray mold of Lilium Longdentiger
(1) Test material
The test pathogen, lilium brownii botrytis, used in this test was from the biological control laboratory of plant protection institute of agriculture university in Hunan.
The reagents used in this experiment were ethylicin (80%, Beijing Zhongnong Jiarui Co., Ltd.), phenazine-1-carboxamide (95%, Shanghai Qinba chemical Co., Ltd.).
Test medium: PDA culture medium (200g potato, 20g glucose, 20g agar, pH is natural, water is added to constant volume to 1L, and sterilization is carried out at 121 deg.C under 1 atmospheric pressure for 20 min).
(2) Single-agent toxicity determination of phenazine-1-formamide and ethylicin
Weighing phenazine-1-formamide and ethylicin in 50mL beakers respectively, adding 2mL of acetone and a proper amount of Tween 80 in the two beakers respectively, shaking and stirring fully until the original drug is completely dissolved, and finally adding deionized water to 100mL respectively to prepare a mother solution of 1000 mu g/mL.
The toxicity of the single agent is tested by adopting a growth rate method. Phenazine-1-formamide and ethylicin are prepared into PDA culture medium containing medicines with the concentration of 6.25, 12.5, 25, 50 and 100 mu g/mL series, a puncher is used for taking a botrytis cinerea cake from the edge of a botrytis cinerea colony cultured for 3d, the botrytis cinerea cake is inoculated on the PDA culture medium containing medicines with the hypha facing downwards, each treatment is repeated for 3 times, and the PDA culture medium containing medicines added with sterile water is used as a blank Control (CK) and is cultured in an inverted mode in an incubator at the temperature of 20 ℃. When the diameter of the control colony was as long as the entire petri dish 2/3, the colony diameter was measured by the cross method, and the inhibition rate was calculated as (control colony diameter-treated colony diameter)/(control colony diameter-cake diameter) × 100%.
The test result is statistically processed by using a DPS data processing platform, and the logarithmic equation of the inhibition rate-dosage (concentration) of each single agent on the toxicity of the gray mold of the lily of the dragon tooth and the related coefficient (R) and the inhibition median concentration (EC) of the single agent on the gray mold of the dragon tooth lily are calculated50)。
(3) Toxicity determination and synergism of phenazine-1-formamide and ethylicin compound
Taking a proper amount of phenazine-1-formamide and ethylicin mother liquor, mixing the phenazine-1-formamide and the ethylicin mother liquor into different mixed agents according to the proportion of 1:9, 2:8, 3:7, 4:6, 1:1, 6:4, 7:3, 2:8 and 1:9 respectively, measuring the toxicity of each mixed agent to the gray mold of the lily agrimony according to the method in the step (2), and calculating the co-toxicity coefficient.
Calculating co-toxicity coefficient: calculating a virulence regression equation and EC as dose log values of the agent and a probability of mortality50(μ g/mL), the cotoxicity coefficient (CotoxldityCoefficient) was calculated according to the calculation method of the combined virulence of bactericides reported in Sun Yunfei et al.
Co-toxicity coefficient (CTC) ═ theoretical virulence index of (measured virulence index of a + B/(a + B) × 100;
the actually measured virulence index (ATI) of (A + B) in the mixture is (A single dose EC)50/(A+B)EC50)×100；
(a + B) a theoretical virulence index (TTI) of agent a x the mass percentage of agent a in the mixture + the virulence index of agent B x the mass percentage of agent B in the mixture;
single agent Toxicity Index (TI) (EC of standard bactericide)50EC for test Unit50)×100。
The combined virulence of the compounded insecticide is shown in 3 conditions, namely synergism, additive and antagonism. If the co-toxicity coefficient is about 100, the additive effect is obviously greater than 120, and the antagonistic effect is obviously less than 80.
(4) Results and analysis
4.1 determination of toxicity of single agents of phenazine-1-carboxamide and ethylicin on Gracilomyces dens
As can be seen from Table 1, the higher the concentrations of the two agents, phenazine-1-carboxamide and ethylicin, the better the bacteriostatic effect. The inhibition effect of phenazine-1-formamide on gray mold of lily of the dragon tooth is better than that of ethylicin. Wherein the regression equation of the toxicity of the phenazine-1-formamide to the gray mold of the lilium agrimoniae is that y is 3.5439+1.0998x, the correlation coefficient is 0.9946, and EC is5021.0859 μ g/mL; the regression equation of the toxicity of ethylicin to the gray mold of the lily of Longya is that y is 1.2124+2.4020x, the correlation coefficient is 0.9961, and EC50138.9683 μ g/mL; the bactericide has better control effect when the concentration of the bactericide is lower, so the phenazine-1-formamide has the strongest toxicity to the gray mold of the lilium agrimonium and has the best control effect.
TABLE 1 inhibition of Botrytis cinerea by phenazine-1-carboxamide and ethylicin
4.2 indoor toxicity assay of combination Agents
As can be seen from Table 2, the inhibition effect of the phenazine-1-carboxamide and ethylicin to Gracilaria verrucosa is proportional to the concentration, and the higher the concentration is, the better the inhibition effect is. The phenazine-1-formamide and ethylicin are compounded in different proportions and have the inhibition effect on the gray mold of the lily of the dragon tooth, wherein the inhibition effect is the best when the ratio is 1: 1. As can be seen from Table 3, the 1:1 combination of phenazine-1-formamide and ethylicin has the strongest toxicity to the gray mold of Lilium Longdentiger, and the second 4:6, and the toxicity effects of the other combinations are 3:7, 1:9, 1:1, 2:8, 6:4, 7:3, 8:2, 9:1, etc. in sequence from strong to weak.
TABLE 2 biological Activity of the combination of phenazine-1-carboxamide and ethylicin on Gray mold of Lilium Longdentiger
TABLE 3 compounding of phenazine-1-carboxamide with ethylicin EC against Gray mold of Lilium Longdentiger50Value of
| Medicament | Equation (y ═) | CorrelationCoefficient R | EC50(μg/mL) |
| Phenazine-1-carboxamide ethylicin 9:1 | y＝3.5592+0.7981x | 0.9820 | 63.8702 |
| Phenazine-1-carboxamide ethylicin 8:2 | y＝3.6829+0.7212x | 0.9958 | 67.0334 |
| Phenazine-1-carboxamide ethylicin 7:3 | y＝3.7767+0.6944x | 0.9936 | 57.7721 |
| Phenazine-1-carboxamide ethylicin 6:4 | y＝3.8308+0.6645x | 0.9848 | 57.4949 |
| Phenazine-1-carboxamide ethylicin 1:1 | y＝3.9466+0.7926x | 0.9914 | 21.3349 |
| Phenazine-1-carboxamide ethylicin 4:6 | y＝3.9947+0.6936x | 0.9946 | 28.1463 |
| Phenazine-1-carboxamide ethylicin 3:7 | y＝3.8816+0.0.7028x | 0.9813 | 39.0253 |
| Phenazine-1-carboxamide ethylicin 2:8 | y＝4.0915+0.5352x | 0.9913 | 49.8176 |
| Phenazine-1-carboxamide ethylicin 1:9 | y＝3.9911+0.6177x | 0.9732 | 42.9494 |
4.3 Co-toxicity coefficient of the combination
As can be seen from Table 4, the co-toxicity coefficient of the liquid medicine prepared from phenazine-1-formamide and ethylicin in a ratio of 1:1, 4:6, 3:7 and 1:9 to the botrytis cinerea of Lilium Longdentle is 172, 153, 133 and 130 in sequence, obvious synergistic effect is shown, and the other proportion of the liquid medicine is compounded to show antagonistic effect.
TABLE 4 Co-toxicity coefficient of phenazine-1-carboxamide and ethylicin against Gracilaria verrucosa
Example 2 in vitro prevention of Gray mold of Longya lily leaves by compounding phenazine-1-carboxamide and ethylicin
(1) Test material
The test pathogen, lilium brownii botrytis, used in this test was from the biological control laboratory of plant protection institute of agriculture university in Hunan.
The reagents used in this experiment were phenazine-1-carboxamide (95%, Shanghai Qinba chemical Co., Ltd.) and 80% ethylicin emulsifiable concentrate (80%, Beijing Zhongnong Jiarui Co., Ltd.), pythium fungicide (95%, Shanghai Qinba chemical Co., Ltd.).
Test medium: PDA culture medium (200g potato, 20g glucose, 20g agar, pH is natural, water is added to constant volume of 1L, and sterilization is carried out at 121 deg.C under 1 atmosphere for 20 min).
(2) In vitro control effect
The phenazine-1-formamide, ethylicin and the compound of the two are respectively prepared into the concentrations of 0.210859mg/mL, 1.389683mg/mL and 0.213349mg/mL for later use. The test was conducted with 3 treatments, 1 drug control (0.80mg/mL), 1 blank control, and 3 treatments were phenazine-1-carboxamide treatment, ethylicin treatment, and phenazine-1-carboxamide and ethylicin complex treatment, the control drug was procymidone, and sterile water was used as the blank control. Uniformly and uniformly soaking the lily leaves of the young dragon teeth with a medicament for 30s respectively, airing, inoculating a lily fungus cake of gray mold of the dragon teeth in the center of the leaves, repeating 3 leaves for each treatment, randomly distributing different treatments, measuring the total length of disease spots 72 hours after the medicament application, and calculating the prevention and treatment effect (%) (control disease spot length-treatment disease spot length)/(control disease spot length-fungus cake diameter)) multiplied by 100%.
Results and analysis: as can be seen from Table 5, the control effect of 1:1 combination of phenazine-1-formamide and ethylicin on gray mold of Lilium Longdentiger can reach 83.85%, which is 1.51 and 1.48 times of the single use of phenazine-1-formamide and ethylicin, and the control agent has better effect than the control agent procymidone and has better application potential.
TABLE 5 in vitro prevention and treatment effects of phenazine-1-carboxamide and ethylicin combination on Gray mold of densa draconis and lily leaf
Claims (1)
1. The application of a compound bactericide containing phenazine-1-formamide and ethylicin in preventing and treating gray mold of lily of Longya is disclosed, wherein the compound bactericide contains phenazine-1-formamide and ethylicin, and the weight ratio of phenazine-1-formamide to ethylicin is 1: 1.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810999000.5A CN109090117B (en) | 2018-08-30 | 2018-08-30 | Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof |
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| CN201810999000.5A CN109090117B (en) | 2018-08-30 | 2018-08-30 | Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof |
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| CN109090117A CN109090117A (en) | 2018-12-28 |
| CN109090117B true CN109090117B (en) | 2021-05-04 |
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| CN201810999000.5A Expired - Fee Related CN109090117B (en) | 2018-08-30 | 2018-08-30 | Compound bactericide containing phenazine-1-formamide and ethylicin and application thereof |
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| CN113197224A (en) * | 2021-05-17 | 2021-08-03 | 淮阴工学院 | Synergistic compound for preventing and treating gray mold of strawberries and application thereof |
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