CN108148777A - A kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application - Google Patents
A kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application Download PDFInfo
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Abstract
The invention belongs to organic substrate fields, and in particular to a kind of bacillus subtilis, complex micro organism fungicide, organic substrate and its preparation and application.The bacillus subtilis of the present inventionBacillus subtilis BS W1, preserving number are：CCTCC M 2017589.The bacillus subtilis can be prepared into complex micro organism fungicide, carry out During High-Temperature Composting, and then be prepared into organic substrate.Since the bacillus subtilis strain has stronger tolerance under 70 DEG C of fermentation conditions, and the larger molecular organics such as protein, starch, the cellulose in a variety of hydrolase efficient degradation raw materials can be secreted, compost central temperature can be made to rise to 70 DEG C.Therefore more conventional composting technology, improves compost fermentation temperature, so as to significantly improve organic matter and total nutrient content, and can more effectively kill pathogen, parasitic ovum, weed seed etc..
Description
Technical field
The invention belongs to organic substrate fields, and in particular to a kind of bacillus subtilis, complex micro organism fungicide, organic group
Matter and its preparation and application.
Background technology
Organic mass cultivation refers to using organic matter such as feces of livestock and poultry, agricultural crop straw, mushroom slag, turf, sawdust etc., through height
It after warm fermentation process, mixes according to a certain percentage, forms a full nutrition cultivation matrix for stablizing and having relatively cushioning effect
Raw material.The cultivation matrix all prepared using organic matter, gas permeability, water-retaining property are bad, therefore in order to improve cultivation matrix
Physicochemical property, can be mixed by a certain percentage by inorganic matters such as vermiculite, perlite, river sand, cinders, and composition organic-inorganic type is planted
Train matrix.
It is at present to overcome greenhouse continuous cropping obstacle most effective, most economical, most thorough using the cultivation technique without soil of cultivation matrix
Method.Therefore the technology is also greatly developed in some external advanced agricultural countries.Dutch 1994 in 4000 public affairs
More than 40% using substrate culture technology in hectare chamber crop, and Israel's greenhouse vegetable has 7% to use soilless culture, and China
Soilless culture scale falls behind compared to more.A key factor for restricting China's soilless culture development is that matrix cost is excessively high.For
It solves the problems, such as this, prepares organic substrate for raw material using agriculture wastes, be a main developing direction.It can be used for
The agriculture wastes of organic substrate raw material mainly have：Animal waste, agricultural crop straw, processing of farm products waste,
Sawdust, rice husk, edible fungi substrate waste material etc..Raw material production organic substrate is done using these agriculture wastes, it on the one hand can be big
The big production cost for reducing cultivation matrix, on the other hand also takes full advantage of organic waste resource, reduces the dirt to environment
Dye.
High temperature aerobic composting technology is the key technology of agriculture and animal husbandry organic waste production organic substrate and realizes that its is harmless
Change, minimizing, recycling treatment effective way.High temperature aerobic composting is to carry out high-temperature solid fermentation, compost using aerobic bacteria
Temperature maintains at least 5 days or more at 50 DEG C or more, and high temperature kills pathogen, parasitic ovum, weed seed, is dropped organic matter
Solve and be eventually converted into the process of stable humus.But it is frequently present of two in conventional high temperature aerobic composting technology and asks
Topic：Although 1) raw material is rich in organic matter and some nutrients, these organic matters are all difficult to degrade big points such as cellulose, protein
Sub- organic matter, since available nutrient is inadequate, C/N is than excessively high, compost microbe is difficult to obtain within (24 or 48 hours) in a short time
It is suitble to the nutrition of their fast-growths, and if the compost microbe strain added in cannot (5-7 days) life rapidly in a short time
It is long, strain failure is may result in, here it is the compost bacteriums often occurred to start the inapparent phenomenon of compost effect.2) due to
Early period microorganism could not raised growth, therefore cannot effectively decompose larger molecular organics, i.e. compost temperature within 48 hours cannot
Rise very rapidly up to 60 DEG C, cause raw material that can not thoroughly be sufficiently carried out fermenting, the too high levels of organic matter in compost product and lead
Cause burn seedlings.
More than technical problem is based on, research and development are a kind of to be made compost microbe fast-growth, quickly start having for composting process
Machine base starting material formula, is the key that solve the problems, such as.
Invention content
In order to overcome the problems of in the prior art, the purpose of the present invention is to provide a kind of bacillus subtilis,
Complex micro organism fungicide, organic substrate and its preparation and application.Aim to solve the problem that microorganism is opened in existing high temperature aerobic composting technology
The inapparent problem of dynamic compost effect.
To achieve these goals and other related purposes, the present invention adopt the following technical scheme that：
The first aspect of the present invention provides a kind of bacillus subtilis Bacillus subtilis BS-W1, preserving number
For：CCTCC M 2017589.
The present invention screens from High Temperature Composting of Livestock Manure raw material and obtains a bacillus subtilis Bacillus
Subtilis BS-W1, on October 19th, 2017 in China typical culture collection center preservation, preserving number is the bacterial strain
CCTCC M 2017589。
The bacillus subtilis that the present invention detaches is resistant to During High-Temperature Composting environment (70 DEG C).And a variety of enzymes can be secreted
The composting materials such as class efficient degradation excrement and stalk.
Through Morphological Identification and the development tree analysis of 16S rRNA, ITS-2 and rbcL extension increasing sequence, it is withered to determine the bacterial strain
Careless bacillus is (according to Intemational Nomenclature rule：Generic name+kind of name+strain name is named the bacterial strain, generic name, kind name, strain name difference
For Bacillus, subtilis and BS-W1), Bacillus subtilis BS-W1 are named as, preserving number is：CCTCC M
2017589.The bacterial strain has following advantage：There is stronger tolerance (70 DEG C) to hot environment.A variety of enzymes can be secreted
The composting materials such as class efficient degradation excrement and stalk.
The second aspect of the present invention provides aforementioned bacillus subtilis Bacillus subtilis BS-W1 for high temperature
The purposes of aerobic compost.
The third aspect of the present invention provides a kind of high temperature aerobic composting complex micro organism fungicide, including bacillus subtilis
Bacterium, aspergillus niger, bacillus amyloliquefaciens, candida tropicalis, trichoderma reesei, ash slightly red streptomyces, Phanerochaete chrysosporium, glimmering
Light pseudomonad, Lactobacillus brevis, long lysine bacillus.
The preserving number of the bacillus subtilis is CCTCC M 2017589.
The entitled Aspergillus niger of Latin of the aspergillus niger.It can be obtained from Culture Collection Center.
The entitled Bacillus amyloliquefaciens of Latin of the bacillus amyloliquefaciens.It can be from culture presevation
The heart obtains.
The entitled Candida tropicalis of Latin of the candida tropicalis.It can be obtained from Culture Collection Center.
The entitled Trichoderma reesei of Latin of the trichoderma reesei.It can be obtained from Culture Collection Center.
The entitled Streptomyces griseorubens of Latin of the ash slightly red streptomyces.It can be from Culture Collection Center
It obtains.
The entitled Phanerochaete chrysosporium of Latin of the Phanerochaete chrysosporium.It can be from culture presevation
Center obtains.
The entitled Psdeuomnoda fluoerncnet of Latin of the Pseudomonas fluorescens.It can be obtained from Culture Collection Center
.
The entitled Lactobacillus breris of Latin of the Lactobacillus brevis.It can be obtained from Culture Collection Center.
The entitled Lysinibacillus macroides of Latin of the long lysine bacillus.It can be from culture presevation
The heart obtains.
Further, in the complex micro organism fungicide, the parts by weight ratio range between each constituent is：
1~5 parts by weight of bacillus subtilis, 1~5 parts by weight of aspergillus niger, 1~5 parts by weight of bacillus amyloliquefaciens, the torrid zone
1~5 parts by weight of Candida, 1~3 parts by weight of trichoderma reesei, ash slightly 1~3 parts by weight of red streptomyces, Phanerochaete chrysosporium 1
~3 parts by weight, 1~3 parts by weight of Pseudomonas fluorescens, 1~3 parts by weight of Lactobacillus brevis, long 1~3 weight of lysine bacillus
Part.
The fourth aspect of the present invention provides the preparation method of afore-said hot aerobic compost complex micro organism fungicide, including
Step：Each constituent is uniformly mixed.
The fifth aspect of the present invention provides afore-said hot aerobic compost complex micro organism fungicide in organic substrate is prepared
Purposes.
The sixth aspect of the present invention, provides a kind of organic substrate, and the organic substrate includes aforementioned complex micro organism fungicide.
Further, a kind of organic substrate is provided, includes the constituent of following parts by weight：
Further, it is appropriate that breeding wastewater RO concentrates are further included.
The discarded fermenting bed padding refers to livestock and poultry cultivation fermenting bed padding.Predominantly pig raising and cowboying fermenting bed padding,
Its main component is sawdust, rice husk, stalk etc..
The solid manure refers to the excrement of feces of livestock and poultry, predominantly pig, ox, chicken etc..
The pomace refers to the remaining pulp and pericarp after fruit squeezing processing fruit juice.May be selected from apple pomace, pears slag,
Citrus pulp, shaddock slag, peach slag etc. it is any one or more.Rich in quick-acting carbon such as glucose, fructose, sucrose, citric acids in pomace
Source for the long-acting carbon source such as cellulose, can mushroom out strain in a short time, quickly start composting process.
The charcoal refers to that feces of livestock and poultry carbonizes the charcoal of preparation under the conditions of high temperature.
The rice chaff can be obtained by approach purchased in market.
The turf can be obtained by approach purchased in market.
The perlite can be obtained by approach purchased in market.
The breeding wastewater RO concentrates refer to that livestock breeding wastewater handles obtained RO through bi-membrane method (ultrafiltration membrane, RO films)
Concentrate.Breeding wastewater through bi-membrane method treated RO permeate can qualified discharge, remaining RO concentrates are small rich in ammonia nitrogen etc.
Molecular organic, it is impossible to which direct emission is often flowed back into stoste and handled again.However the ammonia nitrogen in RO concentrates is micro- life
The quick-acting nitrogen sources of object growth, equally can mushroom out strain in a short time, quickly start composting process.
The seventh aspect of the present invention provides the preparation method of aforementioned organic substrate, includes the following steps：
(1) after discarded fermenting bed padding is dried, excrement therein and bedding and padding are uniformly mixed, crushed, it is spare；
(2) the discarded pomace after drying crushes, spare；
(3) solid manure is dried, crushes, charing process is carried out under 500-600 DEG C of anoxia condition, obtains charcoal；
(4) pomace after discarded fermenting bed padding, solid manure, step (2) crushing after step (1) is crushed, raw step
Suddenly charcoal, rice chaff are uniformly mixed according to a certain percentage obtained by (3)；
(5) breeding wastewater RO concentrates are sprayed on step (4) resulting material, be uniformly mixed；
(6) by proportioning complex micro organism fungicide is added in into mixed material obtained by step (5)；
(7) fermentation maturity is carried out, fermentation maturity terminates, dries；
(8) the good material of fermentation maturity and turf, perlite are mixed according to a certain percentage, control moisture is less than
30wt%, sub-sieve, packaging are to get organic substrate.
Preferably, in step (1), grain size is crushed to as 1-2mm.
Preferably, in step (2), grain size is crushed to as 1-2mm.
Preferably, in step (3), grain size is crushed to as 1-2mm.
Preferably, in step (3), processing time 1-3h.Further preferably handle 2h.
Preferably, in step (5), the moisture of material is in the range of 55-70wt% after control mixing, pH value 6-9.
Preferably, in step (6), complex micro organism fungicide is added in a manner of stirring or spray.
Preferably, it in step (8), is sent out using conventional fermentation cabin compost, bar buttress formula compost or static compost method
Ferment is decomposed, 1.5-2 meters of stacking height, and 1.5-2 meters of width starts turning when temperature of charge rises to 55-60 DEG C, every to turn within 2-5 days
Heap is primary, and control fermentation temperature is 55-70 DEG C, and fermentation time is 10-40 days, when fermentation temperature no longer increases, compost volume is collapsed
When falling into 1/3~1/2, fermentation maturity terminates.
The eighth aspect of the present invention provides purposes of the aforementioned organic substrate for proportion of crop planting.
Compared with prior art, the present invention has the advantages that：
(1) present invention has used pomace and breeding wastewater RO concentrates in base starting material formula, is carried for compost microbe
Quick-acting carbon sources and nitrogen source have been supplied, it is made raised growth and quickly to start composting process, therefore more conventional high temperature in a short time
Aerobic compost technology shortens the compost maturity time.Both raw materials are respectively that processing of farm products waste and livestock and poultry cultivation give up
Gurry will not additionally increase the production cost of organic substrate, and with apparent compost fermentation effect.
(2) the During High-Temperature Composting fermentation strain voluntarily screened has been used in high temperature aerobic composting technology of the present invention --- withered grass bud
Spore bacillus, which has stronger tolerance under 70 DEG C of fermentation conditions, and it is efficient to secrete a variety of hydrolases
The larger molecular organics such as protein, cellulose in degradation raw material, but compost temperature rises to 70 DEG C.Therefore more conventional height
Warm aerobic compost technology, improves compost fermentation temperature, so as to more effectively kill pathogen, parasitic ovum, weed seed etc..
(3) raw material for preparing of organic substrate of the present invention has used a variety of agriculture and animal husbandry wastes, such as 1) discards fermenting bed padding：It is logical
High temperature compost fermentation is crossed, makes wherein undegradable solid manure fully degraded, and can effectively kill the pathogen in excrement, and
Sawdust, rice husk in bedding and padding can increase water-retaining property and porosity as the component in organic substrate formula；2) pomace：Due to fruit
Rich in nutrition content, water content are high in slag, if not handling immediately, corrupt will be acidified within several hours, grow worm fly, by fruit
Slag is applied in the During High-Temperature Composting fermentation of organic substrate, can make full use of quick-acting carbon sources therein, and can inhibit the life of spoilage organisms
Long breeding；3) charcoal：The charcoal prepared using feces of livestock and poultry is added in organic substrate, and there is loose ventilative, water conservation to protect
Fertilizer, culture beneficial microbe, heavy metal-passivated effect.Organic substrate is produced using these agriculture and animal husbandry wastes, is not only fully sent out
The advantage of various components has been waved, and has been truly realized the innoxious of organic waste, minimizing, recycling treatment.
Bacterial strain preservation information of the present invention is as follows：
Strain name：Bacillus subtilis Bacillus subtilis BS-W1；
Preserving number is：CCTCC NO：M 2017589；
Preservation date：On October 19th, 2017；
Depositary institution's title：China typical culture collection center；
Depositary institution is referred to as：CCTCC；
Depositary institution address：Wuhan, China Wuhan University.
Specific embodiment
Before further describing the specific embodiments of the present invention, it should be appreciated that protection scope of the present invention is not limited to down
State specific specific embodiment；It is also understood that the term used in the embodiment of the present invention is specific specific in order to describe
Embodiment, the protection domain being not intended to be limiting of the invention.The test method of actual conditions is not specified in the following example,
Usually according to normal condition or the condition proposed by according to each manufacturer.
When embodiment provides numberical range, it should be appreciated that except non-present invention is otherwise noted, two ends of each numberical range
Any one numerical value can be selected between point and two endpoints.Unless otherwise defined, in the present invention all technologies for using and
Scientific terminology is identical with the normally understood meaning of those skilled in the art of the present technique.Except used in embodiment specific method, equipment,
Outside material, according to record of the those skilled in the art to the grasp of the prior art and the present invention, it can also use and this
Any method, equipment and the material of the similar or equivalent prior art of method, equipment described in inventive embodiments, material come real
The existing present invention.
The acquisition of 1 bacillus subtilis of embodiment and identification, preservation
The present invention screens from High Temperature Composting of Livestock Manure raw material and obtains a bacillus subtilis Bacillus
Subtilis BS-W1, on October 19th, 2017 in China typical culture collection center preservation, preserving number is the bacterial strain
CCTCC M 2017589。
The bacillus subtilis that the present invention detaches is resistant to During High-Temperature Composting environment (70 DEG C).And a variety of enzymes can be secreted
The composting materials such as class efficient degradation excrement and stalk.
Through Morphological Identification and the development tree analysis of 16S rRNA, ITS-2 and rbcL extension increasing sequence, it is withered to determine the bacterial strain
Careless bacillus is (according to Intemational Nomenclature rule：Generic name+kind of name+strain name is named the bacterial strain, generic name, kind name, strain name difference
For Bacillus, subtilis and BS-W1), Bacillus subtilis BS-W1 are named as, preserving number is：CCTCC M
2017589.The bacterial strain has following advantage：There is stronger tolerance (70 DEG C) to hot environment.A variety of enzymes can be secreted
The composting materials such as class efficient degradation excrement and stalk.
2 high temperature aerobic composting complex micro organism fungicide of embodiment
The bacillus subtilis Bacillus subtilis BS-W1 of the present invention can be used for high temperature aerobic composting.Usually,
Itself and other microorganisms are configured to complex micro organism fungicide, carry out high temperature aerobic composting.
Parts by weight ratio in complex micro organism fungicide 1 between each constituent is ranging from：1 weight of bacillus subtilis
Part, 5 parts by weight of aspergillus niger, 1 parts by weight of bacillus amyloliquefaciens, 5 parts by weight of candida tropicalis, 1 parts by weight of trichoderma reesei, ash
Slightly red streptomyces 3 parts by weight, 1 parts by weight of Phanerochaete chrysosporium, 3 parts by weight of Pseudomonas fluorescens, 1 parts by weight of Lactobacillus brevis,
Long 3 parts by weight of lysine bacillus.
Parts by weight ratio in complex micro organism fungicide 2 between each constituent is ranging from：5 weight of bacillus subtilis
Part, 1 parts by weight of aspergillus niger, 5 parts by weight of bacillus amyloliquefaciens, 1 parts by weight of candida tropicalis, 3 parts by weight of trichoderma reesei, ash
Slightly red streptomyces 1 parts by weight, 3 parts by weight of Phanerochaete chrysosporium, 1 parts by weight of Pseudomonas fluorescens, 3 parts by weight of Lactobacillus brevis,
Long 1 parts by weight of lysine bacillus.
Parts by weight ratio in complex micro organism fungicide 3 between each constituent is ranging from：2 weight of bacillus subtilis
Part, 3 parts by weight of aspergillus niger, 4 parts by weight of bacillus amyloliquefaciens, 3 parts by weight of candida tropicalis, 2 parts by weight of trichoderma reesei, ash
Slightly 2 parts by weight of red streptomyces, 2 parts by weight of Phanerochaete chrysosporium, 2.5 parts by weight of Pseudomonas fluorescens, 2 weight of Lactobacillus brevis
Part, long 2.5 parts by weight of lysine bacillus.
Organic substrate composition of raw materials is：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, apple
20 parts by weight of slag, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite.
Organic substrate 1：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, 20 weight of apple pomace
Part, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite are not added with any composite microbial bacteria
Agent, direct compost maturity；
Organic substrate 2：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, 20 weight of apple pomace
Part, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite, addition complex micro organism fungicide 1, heap
Fertilizer is decomposed.
Organic substrate 3：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, 20 weight of apple pomace
Part, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite, addition complex micro organism fungicide 2, heap
Fertilizer is decomposed.
Organic substrate 4：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, 20 weight of apple pomace
Part, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite, addition complex micro organism fungicide 3, heap
Fertilizer is decomposed.
Table 1
By preparing organic substrate, compost with the complex micro organism fungicide in the application present invention it can be seen from 1 data of upper table
Highest central temperature can reach 70 DEG C in the process, and 55 DEG C of the compost temperature than not applying microbial inoculum significantly improves, and can kill significantly
Harmful microorganism and worm's ovum in machine matrix；Its organic substrate prepared, organic matter and total nutrient content significantly improve, and can
Improve the germination index of seed.
Embodiment 3 prepares organic substrate using cowboying waste and apple pomace
Organic substrate formula in the present embodiment：
The parts by weight of each constitutive material are：Discarded 30 parts by weight of cowboying fermenting bed padding, 30 parts by weight of ox solid manure, apple
20 parts by weight of pomace, 8 parts by weight of charcoal, 6 parts by weight of rice chaff, 3 parts by weight of turf, 3 parts by weight of perlite add in composite microbial
1 parts by weight of object microbial inoculum are fermented.The constituent of complex micro organism fungicide and parts by weight are in the present embodiment：Withered grass gemma
3 parts of bacillus, 5 parts of aspergillus niger, 3 parts of bacillus amyloliquefaciens, 3 parts of candida tropicalis, 3 parts of trichoderma reesei, ash slightly red streptomyces 1
Part, 3 parts of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens, 1 part of Lactobacillus brevis, 1 part of long lysine bacillus.
The preparation method of organic substrate, includes the following steps：
(1) discarded cowboying fermenting bed padding is collected, dries, excrement therein and bedding and padding is uniformly mixed, are crushed to grain size
For 1-2mm；
(2) apple pomace is collected, is dried, is crushed to grain size as 1-2mm；
(3) the ox solid manure after separation of solid and liquid is dried, is crushed to 1-2mm, handled under 500-600 DEG C of anoxia condition
2h obtains charcoal；
(4) discarded cowboying fermenting bed padding, ox solid manure, apple pomace, charcoal, rice chaff are mixed according to formula rate
Uniformly；
(5) cowboying waste water RO concentrates are sprayed on material and be uniformly mixed, the moisture of material exists after control mixing
60%, it is 7 to adjust pH value；
(6) complex micro organism fungicide is uniformly sprinkled upon on material, fermentation maturity, stacking is carried out using bar buttress formula compost method
Highly 1.5 meters, 2 meters of width starts turning when temperature of charge rises to 60 DEG C, primary per turning in 2-5 days, and control fermentation temperature is
60-70 DEG C, fermentation time is 15 days, when fermentation temperature no longer increases, compost volume collapses 1/3~1/2, fermentation maturity knot
Beam dries；
(7) the good material of fermentation maturity and turf, perlite are mixed according to formula rate, control moisture is less than
30wt%, sub-sieve, packaging are to get organic substrate.
Embodiment 4 prepares organic substrate using pig raising waste object and pears slag
Organic substrate formula in the present embodiment：
The parts by weight of each constitutive material are：Discarded fermentation bed for pig raising bedding and padding 40, pig solid manure 20, pears slag 15, charcoal
10, rice chaff 5, turf 1, perlite 5, add in 0.01 parts by weight of complex micro organism fungicide ferment.It is compound micro- in the present embodiment
The constituent and parts by weight of bacteria agent be：5 parts of bacillus subtilis, 3 parts of aspergillus niger, 5 parts of bacillus amyloliquefaciens, heat
Band 1 part of Candida, 2 parts of trichoderma reesei, ash slightly 1 part of red streptomyces, 1 part of Phanerochaete chrysosporium, 1 part of Pseudomonas fluorescens,
1 part of Lactobacillus brevis, 1 part of long lysine bacillus.
There is based substrate preparation method, include the following steps：
(1) discarded fermentation bed for pig raising bedding and padding are collected, dries, excrement therein and bedding and padding is uniformly mixed, are crushed to grain size
For 1-2mm；
(2) pears slag is collected, is dried, is crushed to grain size as 1-2mm；
(3) the pig solid manure after separation of solid and liquid is dried, is crushed to 1-2mm, handled under 500-600 DEG C of anoxia condition
2h obtains charcoal；
(4) discarded fermentation bed for pig raising bedding and padding, pig solid manure, pears slag, charcoal, rice chaff are mixed according to formula rate equal
It is even；
(5) Pig raising wastewater RO concentrates are sprayed on material and be uniformly mixed, the moisture of material exists after control mixing
55%, it is 9 to adjust pH value；
(6) complex micro organism fungicide is uniformly sprinkled upon on material, fermentation maturity, stacking is carried out using bar buttress formula compost method
Highly 2 meters, 1.5 meters of width starts turning when temperature of charge rises to 55 DEG C, primary per turning in 2-5 days, and control fermentation temperature is
55-70 DEG C, fermentation time is 10 days, when fermentation temperature no longer increases, compost volume collapses 1/3~1/2, fermentation maturity knot
Beam dries；
(7) the good material of fermentation maturity and turf, perlite are mixed according to formula rate, control moisture is less than
30wt%, sub-sieve, packaging are to get organic substrate.
Embodiment 5 prepares organic substrate using cowboying pig raising waste object and citrus pulp
Organic substrate formula in the present embodiment：
The parts by weight of each constitutive material are：Discarded 50 parts by weight of cowboying fermenting bed padding, 10 parts by weight of pig solid manure, mandarin orange
10 parts by weight of tangerine slag, charcoal 5,10 parts by weight of rice chaff, 5 parts by weight of turf, 1 parts by weight of perlite add in composite microbial bacteria
2 parts by weight of agent are fermented.The constituent of complex micro organism fungicide and parts by weight are in the present embodiment：Bacillus subtilis
1 part, 1 part of aspergillus niger, 1 part of bacillus amyloliquefaciens, 5 parts of candida tropicalis, 1 part of trichoderma reesei, ash slightly 3 parts of red streptomyces,
3 parts of Phanerochaete chrysosporium, 3 parts of Pseudomonas fluorescens, 3 parts of Lactobacillus brevis, 3 parts of long lysine bacillus.
Organic substrate preparation method, includes the following steps：
(1) discarded cowboying fermenting bed padding is collected, dries, excrement therein and bedding and padding is uniformly mixed, are crushed to grain size
For 1-2mm；
(2) citrus pulp is collected, is dried, is crushed to grain size as 1-2mm；
(3) the pig solid manure after separation of solid and liquid is dried, is crushed to 1-2mm, handled under 500-600 DEG C of anoxia condition
2h obtains charcoal；
(4) discarded cowboying fermenting bed padding, pig solid manure, citrus pulp, charcoal, rice chaff are mixed according to formula rate
Uniformly；
(5) Pig raising wastewater RO concentrates are sprayed on material and be uniformly mixed, the moisture of material exists after control mixing
70%, it is 6 to adjust pH value；
(6) complex micro organism fungicide is uniformly sprinkled upon on material, fermentation maturity, stacking is carried out using bar buttress formula compost method
Highly 1.5 meters, 2 meters of width starts turning when temperature of charge rises to 60 DEG C, primary per turning in 2-5 days, and control fermentation temperature is
65-70 DEG C, fermentation time is 40 days, when fermentation temperature no longer increases, compost volume collapses 1/3~1/2, fermentation maturity knot
Beam dries；
(7) the good material of fermentation maturity and turf, perlite are mixed according to formula rate, control moisture is less than
30wt%, sub-sieve, packaging are to get organic substrate.
The different organic substrate effects of embodiment 6 compare
In same greenhouse, strawberry is planted with the organic substrate prepared by embodiment 3-5 respectively, control group 1 is soil,
Control group 2 is that (parts by weight of each constitutive material are for the component proportion of embodiment 1：Discarded cowboying fermenting bed padding 30, ox solid excrement
Just 30, apple pomace 20, charcoal 8, rice chaff 6, turf 3, perlite 3), but the complex micro organism fungicide of this patent is not added, lead to
Cross the organic substrate obtained by Natural compost.
Same kind is cultivated, daily management measure is identical.Application effect is as follows：
As can be seen from the above data, the organic substrate prepared in the application present invention using animal waste and pomace
Afterwards, strawberry can increase production 12.6-20.4%, and soluble solid content increases than control, and soluble sugar content also increased, can
Titrable acid is declined slightly.It is all increased using organic substrate plantation yield and quality of strawberry.
The above, only presently preferred embodiments of the present invention, not to the present invention in any form with substantial limitation,
It should be pointed out that for those skilled in the art, under the premise of the method for the present invention is not departed from, can also make
Several improvement and supplement, these are improved and supplement also should be regarded as protection scope of the present invention.All those skilled in the art,
Without departing from the spirit and scope of the present invention, when made using disclosed above technology contents it is a little more
Dynamic, modification and the equivalent variations developed, are the equivalent embodiment of the present invention；Meanwhile all substantial technologicals pair according to the present invention
The variation, modification and evolution of any equivalent variations that above-described embodiment is made still fall within the range of technical scheme of the present invention
It is interior.
Claims (12)
1. a kind of bacillus subtilis, preserving number is CCTCC M 2017589.
2. bacillus subtilis as described in claim 1 is used for the purposes of high temperature aerobic composting.
3. a kind of complex micro organism fungicide, including bacillus subtilis, aspergillus niger, bacillus amyloliquefaciens, candida tropicalis,
Trichoderma reesei, ash slightly red streptomyces, Phanerochaete chrysosporium, Pseudomonas fluorescens, Lactobacillus brevis, long lysine bacillus.
4. complex micro organism fungicide according to claim 3, which is characterized in that the preserving number of the bacillus subtilis is
CCTCC M 2017589。
5. complex micro organism fungicide according to claim 3, which is characterized in that in the complex micro organism fungicide, each group
It is into the parts by weight ratio range between ingredient：1~5 parts by weight of bacillus subtilis, 1~5 parts by weight of aspergillus niger, solution starch
1~5 parts by weight of bacillus, 1~5 parts by weight of candida tropicalis, 1~3 parts by weight of trichoderma reesei, ash slightly red streptomyces 1~3
Parts by weight, 1~3 parts by weight of Phanerochaete chrysosporium, 1~3 parts by weight of Pseudomonas fluorescens, 1~3 parts by weight of Lactobacillus brevis, length
1~3 parts by weight of lysine bacillus.
6. the preparation method of complex micro organism fungicide as described in claim any one of 3-5, including step：Each constituent is equal
Even mixing.
7. purposes of the complex micro organism fungicide in organic substrate is prepared as described in claim any one of 3-5.
8. a kind of organic substrate includes the constituent of following parts by weight：
9. organic substrate according to claim 8, which is characterized in that it is appropriate to further include breeding wastewater RO concentrates.
10. the preparation method of organic substrate as claimed in claim 8 or 9, which is characterized in that include the following steps：
(1) after discarded fermenting bed padding is dried, excrement therein and bedding and padding are uniformly mixed, crushed, it is spare；
(2) the discarded pomace after drying crushes, spare；
(3) solid manure is dried, crushes, charing process is carried out under 500-600 DEG C of anoxia condition, obtains charcoal；
(4) pomace after discarded fermenting bed padding, solid manure, step (2) crushing after step (1) is crushed, step (3) institute
Obtain charcoal, rice chaff is uniformly mixed according to a certain percentage；
(5) breeding wastewater RO concentrates are sprayed on step (4) resulting material, be uniformly mixed；
(6) by proportioning complex micro organism fungicide is added in into mixed material obtained by step (5)；
(7) fermentation maturity is carried out, fermentation maturity terminates, dries；
(8) the good material of fermentation maturity and turf, perlite are mixed according to a certain percentage, control moisture is less than
30wt%, sub-sieve, packaging are to get organic substrate.
11. preparation method as claimed in claim 10 further includes any one of following characteristics or multinomial：A) in step (1),
Grain size is crushed to as 1-2mm；B) in step (2), grain size is crushed to as 1-2mm；C) in step (3), grain size is crushed to as 1-
2mm；D) processing time is 1-3h in step (3)；E) in step (5), the moisture of material is in 55-70wt% after control mixing
In the range of, pH value 6-9；F) in step (6), complex micro organism fungicide is added in a manner of stirring or spray；G) in step (8),
Fermentation maturity is carried out using conventional fermentation cabin compost, bar buttress formula compost or static compost method, 1.5-2 meters of stacking height is wide
1.5-2 meters of degree, starts turning when temperature of charge rises to 55-60 DEG C, primary per turning in 2-5 days, and control fermentation temperature is 55-70
DEG C, fermentation time is 10-40 days, and when fermentation temperature no longer increases, compost volume collapses 1/3~1/2, fermentation maturity terminates.
12. organic substrate as claimed in claim 8 or 9 is used for the purposes of proportion of crop planting.
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Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN110669705A (en) * | 2019-11-19 | 2020-01-10 | 曲阜师范大学 | Lysinibacillus fusiformis CA1 and application thereof in promoting growth of pleurotus geesteranus |
| CN111019869A (en) * | 2019-12-31 | 2020-04-17 | 广东希普生物科技股份有限公司 | Efficient cotton straw decomposition agent and preparation method thereof |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106350469A (en) * | 2016-11-09 | 2017-01-25 | 上海交通大学 | Bacillus with high temperature resistance and cellulose degradation capacity and application thereof |
| CN106434417A (en) * | 2016-08-01 | 2017-02-22 | 奥为（天津）环保科技有限公司 | High-temperature-resistant cellulase producing bacterium and application thereof |
-
2018
- 2018-01-10 CN CN201810024114.8A patent/CN108148777B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106434417A (en) * | 2016-08-01 | 2017-02-22 | 奥为（天津）环保科技有限公司 | High-temperature-resistant cellulase producing bacterium and application thereof |
| CN106350469A (en) * | 2016-11-09 | 2017-01-25 | 上海交通大学 | Bacillus with high temperature resistance and cellulose degradation capacity and application thereof |
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| CN110669705A (en) * | 2019-11-19 | 2020-01-10 | 曲阜师范大学 | Lysinibacillus fusiformis CA1 and application thereof in promoting growth of pleurotus geesteranus |
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