CA2772964C - Procede de preparation de proteines derivees de plantes - Google Patents
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- CA2772964C CA2772964C CA2772964A CA2772964A CA2772964C CA 2772964 C CA2772964 C CA 2772964C CA 2772964 A CA2772964 A CA 2772964A CA 2772964 A CA2772964 A CA 2772964A CA 2772964 C CA2772964 C CA 2772964C
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- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
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- 150000003905 phosphatidylinositols Chemical class 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 229920002401 polyacrylamide Polymers 0.000 description 1
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 description 1
- 229920000768 polyamine Polymers 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
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- 230000003389 potentiating effect Effects 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 235000013594 poultry meat Nutrition 0.000 description 1
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- 239000011546 protein dye Substances 0.000 description 1
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- 235000005974 protein supplement Nutrition 0.000 description 1
- 229940116540 protein supplement Drugs 0.000 description 1
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- 239000000018 receptor agonist Substances 0.000 description 1
- 229940044601 receptor agonist Drugs 0.000 description 1
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- 238000011069 regeneration method Methods 0.000 description 1
- 238000012552 review Methods 0.000 description 1
- 210000003705 ribosome Anatomy 0.000 description 1
- XYSQXZCMOLNHOI-UHFFFAOYSA-N s-[2-[[4-(acetylsulfamoyl)phenyl]carbamoyl]phenyl] 5-pyridin-1-ium-1-ylpentanethioate;bromide Chemical compound [Br-].C1=CC(S(=O)(=O)NC(=O)C)=CC=C1NC(=O)C1=CC=CC=C1SC(=O)CCCC[N+]1=CC=CC=C1 XYSQXZCMOLNHOI-UHFFFAOYSA-N 0.000 description 1
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- 230000035945 sensitivity Effects 0.000 description 1
- 125000003607 serino group Chemical group [H]N([H])[C@]([H])(C(=O)[*])C(O[H])([H])[H] 0.000 description 1
- 238000010008 shearing Methods 0.000 description 1
- 230000019491 signal transduction Effects 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- NLJMYIDDQXHKNR-UHFFFAOYSA-K sodium citrate Chemical compound O.O.[Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NLJMYIDDQXHKNR-UHFFFAOYSA-K 0.000 description 1
- 239000002195 soluble material Substances 0.000 description 1
- 239000000600 sorbitol Substances 0.000 description 1
- 235000014347 soups Nutrition 0.000 description 1
- 150000003408 sphingolipids Chemical class 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 235000000346 sugar Nutrition 0.000 description 1
- 150000005846 sugar alcohols Chemical class 0.000 description 1
- 150000008163 sugars Chemical class 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- UEUXEKPTXMALOB-UHFFFAOYSA-J tetrasodium;2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O UEUXEKPTXMALOB-UHFFFAOYSA-J 0.000 description 1
- 238000013519 translation Methods 0.000 description 1
- 239000012588 trypsin Substances 0.000 description 1
- 229950009811 ubenimex Drugs 0.000 description 1
- 108020005087 unfolded proteins Proteins 0.000 description 1
- VBEQCZHXXJYVRD-GACYYNSASA-N uroanthelone Chemical compound C([C@@H](C(=O)N[C@H](C(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CS)C(=O)N[C@H](C(=O)N[C@@H]([C@@H](C)CC)C(=O)NCC(=O)N[C@@H](CC=1C=CC(O)=CC=1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CS)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H]([C@@H](C)O)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CC=1C2=CC=CC=C2NC=1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(N)=N)C(O)=O)C(C)C)[C@@H](C)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CCSC)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)CNC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CS)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CS)NC(=O)CNC(=O)[C@H]1N(CCC1)C(=O)[C@H](CC=1C=CC(O)=CC=1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(N)=O)C(C)C)[C@@H](C)CC)C1=CC=C(O)C=C1 VBEQCZHXXJYVRD-GACYYNSASA-N 0.000 description 1
- 238000002255 vaccination Methods 0.000 description 1
- 229940125575 vaccine candidate Drugs 0.000 description 1
- 239000013598 vector Substances 0.000 description 1
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 108010082737 zymolyase Proteins 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N ฮฒโMercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Classifications
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- CโCHEMISTRY; METALLURGY
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- C07KโPEPTIDES
- C07K14/00โPeptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415โPeptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- AโHUMAN NECESSITIES
- A61โMEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61KโPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00โMedicinal preparations containing antigens or antibodies
- A61K39/12โViral antigens
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- AโHUMAN NECESSITIES
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- A61K39/12โViral antigens
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- AโHUMAN NECESSITIES
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- A61P31/00โAntiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- AโHUMAN NECESSITIES
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- A61P31/00โAntiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/12โAntivirals
- A61P31/14โAntivirals for RNA viruses
- A61P31/16โAntivirals for RNA viruses for influenza or rhinoviruses
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- AโHUMAN NECESSITIES
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- A61P37/00โDrugs for immunological or allergic disorders
- A61P37/02โImmunomodulators
- A61P37/04โImmunostimulants
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- C07KโPEPTIDES
- C07K1/00โGeneral methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
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- C07K1/34โExtraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
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- C12N15/09โRecombinant DNA-technology
- C12N15/63โIntroduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79โVectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82โVectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201โMethods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
- C12N15/8202โMethods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
- C12N15/8203โVirus mediated transformation
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- C12N15/09โRecombinant DNA-technology
- C12N15/63โIntroduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79โVectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82โVectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241โPhenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242โPhenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257โPhenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
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- C12N15/63โIntroduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79โVectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82โVectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241โPhenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242โPhenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8257โPhenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
- C12N15/8258โPhenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon for the production of oral vaccines (antigens) or immunoglobulins
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- A61โMEDICAL OR VETERINARY SCIENCE; HYGIENE
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- A61K2039/51โMedicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
- A61K2039/517โPlant cells
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- A61โMEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61KโPREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00โMedicinal preparations containing antigens or antibodies
- A61K2039/51โMedicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
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- C12N2760/16011โOrthomyxoviridae
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- C12N2760/16011โOrthomyxoviridae
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Abstract
L'invention concerne des procรฉdรฉs de prรฉparation de protรฉines ou protรฉines ร suprastructure dรฉrivรฉes de plantes. Le procรฉdรฉ peut comprendre l'obtention d'une plante ou d'une matiรจre vรฉgรฉtale comprenant des protรฉines ou protรฉines ร suprastructure localisรฉes dans l'apoplaste, la production d'une fraction protoplaste/sphรฉroplaste et d'une fraction apoplaste ร partir de la plante ou de la matiรจre vรฉgรฉtale, et la rรฉcupรฉration de la fraction apoplaste. La fraction apoplaste comprend les protรฉines ou protรฉines ร suprastructure dรฉrivรฉes de plantes. En variante, les protรฉines ou protรฉines ร suprastructure peuvent รชtre obtenues ร partir d'une plante ou d'une matiรจre vรฉgรฉtale comprenant des protรฉines ou protรฉines ร suprastructure dรฉrivรฉes de plantes par digestion de la matiรจre vรฉgรฉtale en utilisant une composition enzymatique de dรฉgradation des parois cellulaires pour produire une fraction digรฉrรฉe. La fraction digรฉrรฉe est filtrรฉe pour produire une fraction filtrรฉe, et les protรฉines ou protรฉines ร suprastructure dรฉrivรฉes de plantes sont rรฉcupรฉrรฉes ร partir de la fraction filtrรฉe.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US24478609P | 2009-09-22 | 2009-09-22 | |
US61/244,786 | 2009-09-22 | ||
PCT/CA2010/001489 WO2011035423A1 (fr) | 2009-09-22 | 2010-09-21 | Procรฉdรฉ de prรฉparation de protรฉines dรฉrivรฉes de plantes |
Publications (2)
Publication Number | Publication Date |
---|---|
CA2772964A1 CA2772964A1 (fr) | 2011-03-31 |
CA2772964C true CA2772964C (fr) | 2020-10-27 |
Family
ID=43795254
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2772962A Active CA2772962C (fr) | 2009-09-22 | 2010-09-21 | Procede de preparation de vlp derivees de plantes |
CA2772964A Active CA2772964C (fr) | 2009-09-22 | 2010-09-21 | Procede de preparation de proteines derivees de plantes |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CA2772962A Active CA2772962C (fr) | 2009-09-22 | 2010-09-21 | Procede de preparation de vlp derivees de plantes |
Country Status (27)
Country | Link |
---|---|
US (2) | US11826419B2 (fr) |
EP (3) | EP3354657B1 (fr) |
JP (2) | JP5554414B2 (fr) |
KR (4) | KR20180026562A (fr) |
CN (4) | CN107129973A (fr) |
AR (2) | AR078432A1 (fr) |
AU (2) | AU2010300033B2 (fr) |
BR (2) | BR112012006415B1 (fr) |
CA (2) | CA2772962C (fr) |
DK (3) | DK2480658T3 (fr) |
ES (3) | ES2642631T3 (fr) |
HR (3) | HRP20220478T1 (fr) |
HU (3) | HUE036776T2 (fr) |
IL (4) | IL218393A0 (fr) |
IN (2) | IN2012DN02637A (fr) |
MX (2) | MX2012003373A (fr) |
MY (2) | MY158475A (fr) |
NO (2) | NO2480658T3 (fr) |
NZ (2) | NZ598508A (fr) |
PL (3) | PL2480560T3 (fr) |
PT (3) | PT3354657T (fr) |
RU (2) | RU2567012C2 (fr) |
SG (2) | SG178947A1 (fr) |
SI (3) | SI2480560T1 (fr) |
TR (1) | TR201803560T4 (fr) |
WO (2) | WO2011035423A1 (fr) |
ZA (2) | ZA201202835B (fr) |
Families Citing this family (34)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CA2615372A1 (fr) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Particules semblables au virus grippal a comprenant de l'hemagglutinine |
EP2238253B1 (fr) | 2007-11-27 | 2012-09-12 | Medicago Inc. | Particules pseudovirales (vlp) de la grippe recombinรฉes produites dans des plantes transgรฉniques exprimant l'hรฉmagglutinine |
IN2012DN00650A (fr) | 2009-06-24 | 2015-06-12 | Medicago Inc | |
NO2480658T3 (fr) * | 2009-09-22 | 2018-02-03 | ||
TWI526539B (zh) | 2010-12-22 | 2016-03-21 | ่่ฟ่กไปฝๆ้ๅ ฌๅธ | ๆค็ฉไธญ็็ข้ก็ ๆฏ้ก็ฒ(vlp)็ๆนๆณๅไปฅ่ฉฒๆนๆณ็็ขไนvlp |
TWI620816B (zh) | 2011-03-23 | 2018-04-11 | ่่ฟ่กไปฝๆ้ๅ ฌๅธ | ๆค็ฉ่ก็่็ฝๅๆถๆนๆณ |
WO2012171104A1 (fr) | 2011-06-13 | 2012-12-20 | Medicago Inc. | Production de particules de type virus de la rage dans des plantes |
WO2013044203A2 (fr) | 2011-09-23 | 2013-03-28 | THE UNITED STATES OF AMERICA, as represented by THE SECRETARY, DEPTARTMENT OF HEALTH & HUMAN SERVICES | Nouveaux vaccins ร base de protรฉine hรฉmagglutinine de la grippe |
US11390878B2 (en) | 2011-09-30 | 2022-07-19 | Medicago Inc. | Increasing protein yield in plants |
PL2760882T3 (pl) | 2011-09-30 | 2023-09-11 | Medicago Inc. | Zwiฤkszenie wydajnoลci czฤ steczek wirusowopodobnych w roลlinach |
CN104114696B (zh) * | 2011-12-21 | 2017-05-03 | ้ฟๆฎๆฏๆ้่ดฃไปปๅ ฌๅธ | ไฝฟ็จๅ ทๆๅฏนๆฐด่งฃ้ ถๆๆง็่กฃๅฃณ็vlp็ๆนๆณ |
JP6406655B2 (ja) * | 2012-02-22 | 2018-10-17 | ไธ่ฑๅไบใใผใใใใฏๆ ชๅผไผ็คพ | ๆค็ฉๆง้ฃๅ็ด ๆใฎ่ปๅๆนๆณใ่ปๅ่ฃฝๅคใ่ปๅใใๆค็ฉๆง้ฃๅ็ด ๆใใใณใใใ็จใใ้ฃๅ |
CA2864733C (fr) * | 2012-03-22 | 2018-09-18 | Fraunhofer Usa, Inc. | Particules de type virus comprenant une matrice de proteine a partir d'un virus enveloppe de plante et utilisations de celles-ci |
AU2013312971B2 (en) | 2012-09-05 | 2019-04-18 | Medicago Inc. | Picornavirus-like particle production in plants |
US9617297B2 (en) * | 2012-10-11 | 2017-04-11 | The Regents Of The University Of California | Apoplast wash fluid recovery for improved recombinant endoglucanase extraction in tabacco leaves |
CA2907591A1 (fr) * | 2013-03-28 | 2014-10-02 | Medicago Inc. | Production de particules de type viral de la grippe dans des plantes |
WO2015034042A1 (fr) | 2013-09-06 | 2015-03-12 | ไธ่ฑๅๅญฆๆ ชๅผไผ็คพ | Procรฉdรฉ de production d'une protรฉine ร l'aide d'une plante |
WO2015054639A1 (fr) | 2013-10-11 | 2015-04-16 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Vaccins contre le virus d'epstein-barr |
CA2936350C (fr) | 2014-01-10 | 2023-01-31 | Medicago Inc. | Elements activateurs du cpmv |
RU2731295C2 (ru) | 2014-07-11 | 2020-09-01 | ะะตะดะธะบะฐะณะพ ะะฝะบ. | ะะพะดะธัะธะบะฐัะธั ะฟัะพะดััะธัะพะฒะฐะฝะธั ะฑะตะปะบะพะฒ ะฒ ัะฐััะตะฝะธัั |
US11191727B2 (en) | 2014-12-31 | 2021-12-07 | The United States Of America, As Represented By The Secretary, Department Of Health And Human Services | Multivalent nanoparticle-based vaccines |
JP2019129705A (ja) * | 2016-03-31 | 2019-08-08 | ๆฅๆฌใใฐใ็ฃๆฅญๆ ชๅผไผ็คพ | ๆค็ฉใซ็ฉ่ณชใๅฐๅ ฅใใๆนๆณ |
IL303650B1 (en) | 2016-09-02 | 2024-10-01 | Us Health | Stable group 2 trimers from the stem region of influenza hemagglutinin and their uses |
WO2019104439A1 (fr) | 2017-11-30 | 2019-06-06 | Medicago Inc. | Protรฉines vp1 de norovirus modifiรฉes et protรฉines vp1 de norovirus comprenant des vlp |
TW202016308A (zh) | 2018-06-27 | 2020-05-01 | ๅ ๆฟๅคงๅ่่ฟ่กไปฝๆ้ๅ ฌๅธ | ๆตๆ็ ๆฏ่ก็ๅ้็ด ็ช่ฎ้ซ |
JP7429684B6 (ja) * | 2018-07-13 | 2024-03-15 | ใกใใฃใซใด ใคใณใณใผใใฌใคใใใ | ๆนๅคใใญใฆใคใซใน๏ฝ๏ฝ๏ผใฟใณใใฏ่ณชใใใณๆนๅคใใญใฆใคใซใน๏ฝ๏ฝ๏ผใฟใณใใฏ่ณชใๅซใ๏ฝ๏ฝ๏ฝ |
CN109112093B (zh) * | 2018-09-05 | 2021-11-09 | ไธๆตทไบค้ๅคงๅญฆ | ไธ็ง้่ฟๅ็่ดจไฝ้ซๆๅ็ฆปๅ็ฌๆถ่ฝฌๅ็ๆนๆณ |
CA3137447A1 (fr) * | 2019-04-25 | 2020-10-29 | Flagship Pioneering Innovations Vi, Llc | Compositions et procedes se rapportant a des paquets de messagers vegetaux |
CN110483616B (zh) * | 2019-07-31 | 2021-06-04 | ไธญๅฝๅไธ็งๅญฆ้ขๅไบงๅๅ ๅทฅ็ ็ฉถๆ | ไปๆๆ็ ๅ่็ๆค็ฉ็ป็ปไธญๅ็ฆป็ ๅ่ๅๆณ็่ดจๅคไฝๆๅบ่็ฝ็ๆนๆณ |
CN111060696B (zh) * | 2019-12-27 | 2023-09-08 | ๆนๅๅไธๅคงๅญฆ | ไธ็ง้ไฝๆค็ฉๅฐๅๅญไฟกๅท่ฝๅ้ณๆง็็ๆนๆณ |
CN111718394A (zh) * | 2020-06-28 | 2020-09-29 | ๅนฟ่ฅฟๅฃฎๆ่ชๆฒปๅบๅไธ็งๅญฆ้ข | ไธ็งๅบไบbppๆณ็็่็ป็ปๅๆง่็ฝๆๅๆนๆณ |
CN111826394A (zh) * | 2020-08-07 | 2020-10-27 | ๆตๆฑๅ็ผ่ฏไธ้ๅขๅป่ฏๅผๅๆ้ๅ ฌๅธ | ๆค็ฉ็ฌๆถ่กจ่พพ่ฝฝไฝ็ๆๅปบๅๅบ็จ |
WO2022260849A1 (fr) | 2021-06-09 | 2022-12-15 | Nant Holdings Ip, Llc | Procรฉdรฉs et systรจmes de production d'une protรฉine d'intรฉrรชt dans une plante |
CN114934007B (zh) * | 2022-07-01 | 2023-11-24 | ๅนฟไธ็ๅไธ็งๅญฆ้ขไฝ็ฉ็ ็ฉถๆ | ไธ็ง็่ฏๅ็่ดจไฝ็ๅถๅคๆนๆณ |
Family Cites Families (77)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4945050A (en) | 1984-11-13 | 1990-07-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
US5100792A (en) | 1984-11-13 | 1992-03-31 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues |
US5036006A (en) | 1984-11-13 | 1991-07-30 | Cornell Research Foundation, Inc. | Method for transporting substances into living cells and tissues and apparatus therefor |
JPS62501263A (ja) | 1984-11-29 | 1987-05-21 | ในใฏใชใใในใใฏใชใใใฏใใขใณใใใชใตโใใใใขใฆใณใโใทใจใณ | ่ฑใฐใชใณใทใซๅใฆใคใซใน็ณใฟใณใใฏ่ณชใซ้ข้ฃใใใใชใใใใ้กใใใณๆไฝ |
JPS61265086A (ja) * | 1985-05-21 | 1986-11-22 | Mitsui Toatsu Chem Inc | ใใญใใใฉในใใฎๅน้คๆณ |
US5232833A (en) | 1988-09-14 | 1993-08-03 | Stressgen Biotechnologies Corporation | Accumulation of heat shock proteins for evaluating biological damage due to chronic exposure of an organism to sublethal levels of pollutants |
US6403865B1 (en) | 1990-08-24 | 2002-06-11 | Syngenta Investment Corp. | Method of producing transgenic maize using direct transformation of commercially important genotypes |
ATE142883T1 (de) | 1991-03-28 | 1996-10-15 | Rooperol Na Nv | Zusammensetzungen von phytosterolen mit phytosterolinen als immunmodulatoren |
UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | ะะพะฒะฐัััั ะะณ | ะคัะฐะณะผะตะฝั ะดะฝะบ, ัะบะธะน ะผัััะธัั ะฟะพัะปัะดะพะฒะฝัััั,ัะพ ะบะพะดัั ัะฝัะตะบัะธัะธะดะฝะธะน ะฟัะพัะตัะฝ, ะพะฟัะธะผัะทะพะฒะฐะฝั ะดะปั ะบัะบัััะดะทะธ,ััะฐะณะผะตะฝั ะดะฝะบ, ัะบะธะน ะทะฐะฑะตะทะฟะตััั ะฝะฐะฟัะฐะฒะปะตะฝั ะฑะฐะถะฐะฝั ะดะปั ัะตััะตะฒะธะฝะธ ััะตะฑะปะฐ ะตะบัะฟัะตััั ะทะฒ'ัะทะฐะฝะพะณะพ ะท ะฝะตั ััััะบัััะฝะพะณะพ ะณะตะฝะฐ ะฒ ัะพัะปะธะฝั, ััะฐะณะผะตะฝั ะดะฝะบ, ัะบะธะน ะทะฐะฑะตะทะฟะตััั ัะฟะตัะธัััะฝั ะดะปั ะฟะธะปะบั ะตะบัะฟัะตััั ะทะฒ`ัะทะฐะฝะพะณะพ ะท ะฝะตั ััััะบัััะฝะพะณะพ ะณะตะฝะฐ ะฒ ัะพัะปะธะฝั, ัะตะบะพะผะฑัะฝะฐะฝัะฝะฐ ะผะพะปะตะบัะปะฐ ะดะฝะบ, ัะฟะพััะฑ ะพะดะตัะถะฐะฝะฝั ะพะฟัะธะผัะทะพะฒะฐะฝะพั ะดะปั ะบัะบัััะดะทะธ ะบะพะดัััะพั ะฟะพัะปัะดะพะฒะฝะพััั ัะฝัะตะบัะธัะธะดะฝะพะณะพ ะฟัะพัะตัะฝั, ัะฟะพััะฑ ะทะฐั ะธััั ัะพัะปะธะฝ ะบัะบัััะดะทะธ ัะพะฝะฐะนะผะตะฝัะต ะฒัะด ะพะดะฝััั ะบะพะผะฐั ะธ-ัะบัะดะฝะธะบะฐ |
US6326470B1 (en) | 1997-04-15 | 2001-12-04 | The Penn State Research Foundation | Enhancement of accessibility of cellulose by expansins |
US5762939A (en) | 1993-09-13 | 1998-06-09 | Mg-Pmc, Llc | Method for producing influenza hemagglutinin multivalent vaccines using baculovirus |
GB9414118D0 (en) | 1994-07-13 | 1994-08-31 | Axis Genetics Ltd | Modified plant viruses as vectors of heterologous peptides |
AU6504796A (en) * | 1995-07-20 | 1997-02-18 | Washington State University Research Foundation | Production of secreted foreign polypeptides in plant cell culture |
US5773695A (en) * | 1996-01-26 | 1998-06-30 | North Carolina State University | Plant nuclear scaffold attachment region and method for increasing gene expression in transgenic cells |
US6042832A (en) | 1996-08-28 | 2000-03-28 | Thomas Jefferson University | Polypeptides fused with alfalfa mosaic virus or ilarvirus capsid proteins |
US20010006950A1 (en) | 1998-02-11 | 2001-07-05 | Juha Punnonen | Genetic vaccine vector engineering |
US6489537B1 (en) | 1998-08-07 | 2002-12-03 | The Trustees Of The University Of Pennsylvania | Phytochelatin synthases and uses therefor |
NZ510358A (en) * | 1998-08-11 | 2002-09-27 | Large Scale Biology Corp | Method for recovering proteins from the interstitial fluid of plant tissues |
US6287570B1 (en) | 1998-11-23 | 2001-09-11 | Patricia L. Foley | Vaccine against swine influenza virus |
WO2000037663A2 (fr) | 1998-12-23 | 2000-06-29 | The Samuel Roberts Noble Foundation, Inc. | Procede de transformation de vegetaux |
FR2791358B1 (fr) | 1999-03-22 | 2003-05-16 | Meristem Therapeutics | Promoteurs chimeriques d'expression, cassettes d'expression, plasmides, vecteurs, plantes et semences transgeniques les contenant et leurs methodes d'obtention |
EP1171618A2 (fr) | 1999-04-21 | 2002-01-16 | The Samuel Roberts Noble Foundation | Procede de transformation de plante |
US7125978B1 (en) | 1999-10-04 | 2006-10-24 | Medicago Inc. | Promoter for regulating expression of foreign genes |
WO2002012454A1 (fr) * | 2000-08-08 | 2002-02-14 | The Kitasato Institute | Micrograins de type viral et procede de production correspondant |
DK1362109T3 (da) | 2001-01-18 | 2009-07-27 | Vlaams Interuniv Inst Biotech | Rekombinante oligomere protein-komplekser med forรถget immunogent potentiale |
AU2002324973A1 (en) | 2001-09-14 | 2003-04-01 | Fraunhofer-Gesellschaft Zur Forderung Der Angewandandten Forschung E.V. | Immunoglobulin having particular framework scaffold and methods of making and using |
EP1572910B1 (fr) | 2002-02-13 | 2015-12-02 | Wisconsin Alumni Research Foundation | Signal d'incorporation de vecteurs de virus de la grippe |
US20040121465A1 (en) | 2002-02-14 | 2004-06-24 | Novavax, Inc. | Optimization of gene sequences of virus-like particles for expression in insect cells |
NZ546945A (en) | 2002-03-19 | 2008-04-30 | Plant Res Int Bv | A plant host cell system for the expression of human GnTIII |
KR20060035596A (ko) * | 2003-05-05 | 2006-04-26 | ๋ณด์ด์ค ํฐ์จ ์ธ์คํฐํํธ ํฌ ํ๋ํธ ๋ฆฌ์์น | ์ธ๋์ ์ ์๋์ ์๋ฌผ๋ก๋ถํฐ ์ ๋๋ ๋ฉด์ญ๋ณดํธ์ฑ ์กฐ์ฑ๋ฌผ์์ ์กฐํ๊ธฐ ์ํ ๋ฒกํฐ์ ์ธํฌ |
US20040268442A1 (en) | 2003-05-05 | 2004-12-30 | Dow Agrosciences Llc | Stable immunoprophylactic and therapeutic compositions derived from transgenic plant cells and methods for production |
US7566458B2 (en) | 2003-06-16 | 2009-07-28 | Medimmune, Llc | Influenza hemagglutinin and neuraminidase variants |
US8592197B2 (en) | 2003-07-11 | 2013-11-26 | Novavax, Inc. | Functional influenza virus-like particles (VLPs) |
CN1560227A (zh) * | 2004-02-25 | 2005-01-05 | ็ฆๅปบๅธ่ๅคงๅญฆ | ้จ็็บข็่ปๅ็่ดจไฝๅถๅคไธๅ็ๆๆฏ |
WO2006016380A2 (fr) | 2004-08-13 | 2006-02-16 | Council Of Scientific And Industrial Research | Vaccin contre la rage a base de proteine g chimerique |
US9155483B2 (en) | 2004-12-03 | 2015-10-13 | The Invention Science Fund I, Llc | Vision modification with reflected image |
EP1861506B1 (fr) * | 2005-03-15 | 2015-04-15 | BP Corporation North America Inc. | Cellulases, acides nucleiques codant pour ces cellulases, et procedes de production et d'utilisation de ces cellulases |
PL2374892T3 (pl) | 2005-04-29 | 2018-06-29 | University Of Cape Town | Ekspresja biaลek wirusa w roลlinach |
CN100410378C (zh) | 2005-05-09 | 2008-08-13 | ไธญๅฝๅไธ็งๅญฆ้ข็็ฉๆๆฏ็ ็ฉถๆ | ็ผ็ ็ฆฝๆตๆ่กๅ็ด ็ๅบๅ ๅๅ ถๆค็ฉ่กจ่พพ่ฝฝไฝๅๅบ็จ |
CA2613160A1 (fr) * | 2005-06-24 | 2006-12-28 | Bayer Bioscience N.V. | Methodes servant a modifier la reactivite de parois cellulaires de plantes |
CN101227920A (zh) | 2005-07-19 | 2008-07-23 | ้ถๆฐ็ฏ็ๆๆฏๅ ฌๅธ | ้็ปๆตๆ็ซ่ |
US7871626B2 (en) | 2005-08-04 | 2011-01-18 | St. Jude Children's Research Hospital | Modified influenza virus for monitoring and improving vaccine efficiency |
ES2543056T3 (es) | 2005-10-18 | 2015-08-14 | Novavax, Inc. | Partรญculas funcionales similares al virus de la gripe (VPL) |
EP2360175B1 (fr) * | 2005-11-22 | 2014-07-16 | Novartis Vaccines and Diagnostics, Inc. | Particules de type virus (vlps) de norovirus et de sapovirus |
CA2642054C (fr) | 2006-02-13 | 2017-11-21 | Fraunhofer Usa, Inc. | Antigenes de la grippe, compositions de vaccins et procedes associes |
US7993655B2 (en) | 2006-04-21 | 2011-08-09 | Dow Agrosciences Llc | Vaccine for avian influenza and methods of use |
US8778353B2 (en) | 2006-05-01 | 2014-07-15 | Technovax, Inc. | Influenza virus-like particle (VLP) compositions |
US9511134B2 (en) | 2006-05-18 | 2016-12-06 | Epimmune Inc. | Inducing immune responses to influenza virus using polypeptide and nucleic acid compositions |
US8519113B2 (en) | 2006-05-22 | 2013-08-27 | Plant Bioscience Limited | Bipartite system, method and composition for the constitutive and inducible expression of high levels of foreign proteins in plants |
US20070286873A1 (en) | 2006-05-23 | 2007-12-13 | Williams John V | Recombinant Influenza H5 Hemagluttinin Protein And Nucleic Acid Coding Therefor |
EP2120762A1 (fr) | 2007-01-17 | 2009-11-25 | Lerner Medical Devices, Inc. | Dispositif de photothรฉrapie ร fibres optiques |
US7730950B2 (en) | 2007-01-19 | 2010-06-08 | Halliburton Energy Services, Inc. | Methods for treating intervals of a subterranean formation having variable permeability |
NZ598417A (en) | 2007-06-15 | 2013-06-28 | Centre Nat Rech Scient | Modifying glycoprotein production in plants |
KR100964462B1 (ko) | 2007-07-10 | 2010-06-16 | ์ฑ๊ท ๊ด๋ํ๊ต์ฐํํ๋ ฅ๋จ | ํ์ง์ ํ ์๋ฌผ ์ ๋์ ์กฐ๋ฅ๋ ๊ฐ ๋ฐ์ด๋ฌ์ค ๋ฐฑ์ ๋ฐ ๊ทธ ์ ์กฐ๋ฐฉ๋ฒ |
CA2615372A1 (fr) | 2007-07-13 | 2009-01-13 | Marc-Andre D'aoust | Particules semblables au virus grippal a comprenant de l'hemagglutinine |
RU2358981C2 (ru) * | 2007-08-07 | 2009-06-20 | ะฆะตะฝัั "ะะธะพะธะฝะถะตะฝะตัะธั" ะ ะพััะธะนัะบะพะน ะะบะฐะดะตะผะธะธ ะะฐัะบ | ะฃะฝะธะฒะตััะฐะปัะฝะฐั ะฒะฐะบัะธะฝะฐ ะฟัะพัะธะฒ ะฒะธัััะฐ ะณัะธะฟะฟะฐ ะฟัะธั |
US20110059130A1 (en) | 2007-08-20 | 2011-03-10 | Fraunhofer Usa, Inc. | Prophylactic and therapeutic influenza vaccines, antigens, compositions and methods |
CN101422128B (zh) * | 2007-10-29 | 2011-06-08 | ไธญๅฝๆฐดไบง็งๅญฆ็ ็ฉถ้ข้ปๆตทๆฐดไบง็ ็ฉถๆ | ้ฉฌๅฐพ่ปๅ็่ดจไฝ็ๅ็ฆปๅๅ็ |
EP2238253B1 (fr) | 2007-11-27 | 2012-09-12 | Medicago Inc. | Particules pseudovirales (vlp) de la grippe recombinรฉes produites dans des plantes transgรฉniques exprimant l'hรฉmagglutinine |
CN101909454A (zh) * | 2007-12-28 | 2010-12-08 | ่ทๅ ฐ่ๅๅฉๅๆ้ๅ ฌๅธ | ไป่ถๅๆถ้ฆๅณๅๅ็ฉ็ๆนๆณ |
US8799801B2 (en) | 2008-01-16 | 2014-08-05 | Qualcomm Incorporated | Interactive ticker |
SG187500A1 (en) | 2008-01-21 | 2013-02-28 | Medicago Inc | Recombinant influenza virus-like particles (vlps) produced in transgenic plants expressing hemagglutinin |
EP2294202B1 (fr) | 2008-07-08 | 2015-05-20 | Medicago Inc. | Antigรจnes de la grippe recombinรฉs solubles |
CN101626276A (zh) | 2008-07-10 | 2010-01-13 | ๅไธบๆๆฏๆ้ๅ ฌๅธ | ๅนฟๅๆฟๆขๆนๆณใ่ฃ ็ฝฎๅ็ณป็ป |
SG192503A1 (en) | 2008-07-18 | 2013-08-30 | Medicago Inc | New influenza virus immunizing epitope |
JP5785080B2 (ja) | 2008-08-27 | 2015-09-24 | ใขใชใพใใปใใผใใปใชใใปใชใผใธใงใณใใปใใฉใผใปใขใณใใปใชใณใปใใใผใใปใชใใปใขใชใพใใปในใใคใใปใฆใใใผใทใใฃ๏ผก๏ฝ๏ฝ๏ฝ๏ฝ๏ฝ๏ฝ ๏ผข๏ฝ๏ฝ๏ฝ๏ฝ ๏ผฏ๏ฝ ๏ผฒ๏ฝ ๏ฝ๏ฝ ๏ฝ๏ฝ๏ฝ ๏ผฆ๏ฝ๏ฝ ๏ผก๏ฝ๏ฝ ๏ผฏ๏ฝ ๏ผข๏ฝ ๏ฝ๏ฝ๏ฝ๏ฝ ๏ผฏ๏ฝ ๏ผก๏ฝ๏ฝ๏ฝ๏ฝ๏ฝ๏ฝ ๏ผณ๏ฝ๏ฝ๏ฝ๏ฝ ๏ผต๏ฝ๏ฝ๏ฝ๏ฝ ๏ฝ๏ฝ๏ฝ๏ฝ๏ฝ | ๆค็ฉใซใใใใฏใฏใใณๅใณใขใใฏใญใผใใซๆไฝๆฒป็่ฌใฎ้ซใฌใใซ่ฟ ้็็ฃใฎใใใฎ๏ฝ๏ฝ๏ฝใฌใใชใณใณ็ณป |
US20100167376A1 (en) * | 2008-10-06 | 2010-07-01 | Genvault Corporation | Methods for providing cellular lysates from cell wall-containing samples |
WO2010077712A1 (fr) | 2008-12-09 | 2010-07-08 | Novavax, Inc. | Particule de type viral du virus syncytial respiratoire bovin (vlps) |
MX2011007916A (es) | 2009-01-30 | 2011-11-18 | Adlyfe Inc | Pรฉptidos conformacionalmente dinรกmicos. |
EA201791744A3 (ru) | 2009-06-10 | 2018-07-31 | ะัะฑัััั ะะธะพัะฐัะผะฐ ะะพัะฟะพััะนัะฝ | ะฃะปัััะตะฝะฝะฐั ะปะธะฟะธะดะฝะฐั ะบะพะผะฟะพะทะธัะธั |
SI2440231T1 (sl) | 2009-06-10 | 2020-08-31 | Nono Inc. | Soฤasno dajanje sredstva, vezanega na internalizacijski peptid tat, z inhibitorjem degranulacije mastocitov |
IN2012DN00650A (fr) | 2009-06-24 | 2015-06-12 | Medicago Inc | |
NO2480658T3 (fr) | 2009-09-22 | 2018-02-03 | ||
ES2614527T3 (es) | 2010-11-04 | 2017-05-31 | Medicago Inc. | Sistema de expresiรณn en plantas |
CN103282023B (zh) | 2010-11-05 | 2018-09-21 | ่ฏบ็ฆ็ฆๅ ๆฏ่กไปฝๆ้ๅ ฌๅธ | ็็ฌ็ ็ณ่็ฝ็ ๆฏๆ ท้ข็ฒ(vlp) |
TWI526539B (zh) | 2010-12-22 | 2016-03-21 | ่่ฟ่กไปฝๆ้ๅ ฌๅธ | ๆค็ฉไธญ็็ข้ก็ ๆฏ้ก็ฒ(vlp)็ๆนๆณๅไปฅ่ฉฒๆนๆณ็็ขไนvlp |
TWI620816B (zh) | 2011-03-23 | 2018-04-11 | ่่ฟ่กไปฝๆ้ๅ ฌๅธ | ๆค็ฉ่ก็่็ฝๅๆถๆนๆณ |
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