ATE528396T1 - Look-through-mutagenese zur entwicklung veränderter polypeptide mit verbesserten eigenschaften - Google Patents
Look-through-mutagenese zur entwicklung veränderter polypeptide mit verbesserten eigenschaftenInfo
- Publication number
- ATE528396T1 ATE528396T1 AT05809866T AT05809866T ATE528396T1 AT E528396 T1 ATE528396 T1 AT E528396T1 AT 05809866 T AT05809866 T AT 05809866T AT 05809866 T AT05809866 T AT 05809866T AT E528396 T1 ATE528396 T1 AT E528396T1
- Authority
- AT
- Austria
- Prior art keywords
- polypeptide
- amino acids
- mutagenesis
- libraries
- look
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
- G01N33/6857—Antibody fragments
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
- C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
- C07K2317/565—Complementarity determining region [CDR]
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
- C07K2317/62—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising only variable region components
- C07K2317/622—Single chain antibody (scFv)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/90—Immunoglobulins specific features characterized by (pharmaco)kinetic aspects or by stability of the immunoglobulin
- C07K2317/92—Affinity (KD), association rate (Ka), dissociation rate (Kd) or EC50 value
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Hematology (AREA)
- Microbiology (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Urology & Nephrology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Cell Biology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Food Science & Technology (AREA)
- Plant Pathology (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Peptides Or Proteins (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US58591804P | 2004-07-06 | 2004-07-06 | |
| PCT/US2005/024140 WO2006023144A2 (en) | 2004-07-06 | 2005-07-06 | Look-through mutagenesis for developing altered polypeptides with enhanced properties |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE528396T1 true ATE528396T1 (de) | 2011-10-15 |
Family
ID=35968026
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT05809866T ATE528396T1 (de) | 2004-07-06 | 2005-07-06 | Look-through-mutagenese zur entwicklung veränderter polypeptide mit verbesserten eigenschaften |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US9012369B2 (de) |
| EP (1) | EP1774019B1 (de) |
| JP (2) | JP4939410B2 (de) |
| AT (1) | ATE528396T1 (de) |
| BR (1) | BRPI0513155B1 (de) |
| CA (1) | CA2572917C (de) |
| ES (1) | ES2372503T3 (de) |
| MX (1) | MX2007000105A (de) |
| WO (1) | WO2006023144A2 (de) |
Families Citing this family (68)
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| US9321832B2 (en) | 2002-06-28 | 2016-04-26 | Domantis Limited | Ligand |
| EP2006381B1 (de) | 2006-03-31 | 2016-02-03 | Chugai Seiyaku Kabushiki Kaisha | Verfahren zur kontrolle der blut-pharmakokinetik von antikörpern |
| EP2012829A4 (de) * | 2006-04-24 | 2010-04-21 | Protelix Inc | Verfahren zur herstellung eines viralen impfstoffes und therapeutischer peptid-antigene |
| CA2666599A1 (en) | 2006-08-18 | 2008-02-21 | Ablynx N.V. | Amino acid sequences directed against il-6r and polypeptides comprising the same for the treatment of diseases and disorders associated with il-6-mediated signalling |
| EP2118281A2 (de) | 2007-02-12 | 2009-11-18 | Codexis, Inc. | Struktur-wirkungs-beziehungen |
| EP2392653B1 (de) * | 2007-07-12 | 2014-03-19 | New England Biolabs, Inc. | Restriktionsendonukleasen mit hoher Fidelität |
| JP5566108B2 (ja) * | 2007-09-26 | 2014-08-06 | 中外製薬株式会社 | 抗ｉｌ−６レセプター抗体 |
| EP4339294A3 (de) | 2007-09-26 | 2024-10-16 | Chugai Seiyaku Kabushiki Kaisha | Verfahren zur modifizierung eines isoelektrischen punkts eines antikörpers durch aminosäuresubstitution in cdr |
| MY163473A (en) | 2007-09-26 | 2017-09-15 | Chugai Pharmaceutical Co Ltd | Modified antibody constant region |
| US20110189203A1 (en) | 2007-11-27 | 2011-08-04 | Ablynx N.V. | Immunoglobulin constructs |
| US20110118447A1 (en) * | 2007-12-31 | 2011-05-19 | Xoma Technology Ltd. | Methods and materials for targeted affinity enhancement |
| KR20240113501A (ko) | 2008-04-11 | 2024-07-22 | 추가이 세이야쿠 가부시키가이샤 | 복수 분자의 항원에 반복 결합하는 항원 결합 분자 |
| CA2723277A1 (en) | 2008-05-02 | 2009-11-05 | Novartis Ag | Improved fibronectin-based binding molecules and uses thereof |
| TWI440469B (zh) | 2008-09-26 | 2014-06-11 | Chugai Pharmaceutical Co Ltd | Improved antibody molecules |
| JP5717624B2 (ja) | 2009-03-19 | 2015-05-13 | 中外製薬株式会社 | 抗体定常領域改変体 |
| JP5787446B2 (ja) | 2009-03-19 | 2015-09-30 | 中外製薬株式会社 | 抗体定常領域改変体 |
| WO2010111707A1 (en) | 2009-03-27 | 2010-09-30 | Sapphire Energy, Inc. | Variant isoprenoid producing enzymes and uses thereof |
| US10618964B2 (en) | 2009-04-10 | 2020-04-14 | Ablynx N.V. | Nanobody against IL-6R |
| KR20130119990A (ko) | 2009-04-10 | 2013-11-01 | 아블린쓰 엔.브이. | Ｉｌ－６ｒ에 대한 개선된 아미노산 서열 및 ｉｌ－６ｒ 관련 질환 및 질병의 치료를 위한 그를 포함하는 폴리펩티드 |
| CA2764398A1 (en) | 2009-06-05 | 2010-12-09 | Erik Depla | Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections |
| US8518405B2 (en) | 2009-10-08 | 2013-08-27 | The University Of North Carolina At Charlotte | Tumor specific antibodies and uses therefor |
| WO2011051327A2 (en) | 2009-10-30 | 2011-05-05 | Novartis Ag | Small antibody-like single chain proteins |
| WO2011051466A1 (en) | 2009-11-02 | 2011-05-05 | Novartis Ag | Anti-idiotypic fibronectin-based binding molecules and uses thereof |
| WO2011064382A1 (en) | 2009-11-30 | 2011-06-03 | Ablynx N.V. | Improved amino acid sequences directed against human respiratory syncytial virus (hrsv) and polypeptides comprising the same for the prevention and/or treatment of respiratory tract infections |
| WO2011092233A1 (en) | 2010-01-29 | 2011-08-04 | Novartis Ag | Yeast mating to produce high-affinity combinations of fibronectin-based binders |
| EP3248987A1 (de) | 2010-07-30 | 2017-11-29 | Novartis AG | Fibronectin-cradle-moleküle und bibliotheken |
| EP2619226B1 (de) * | 2010-09-22 | 2018-09-12 | Amgen Inc. | Träger-immunglobuline und verwendungen davon |
| US9845362B2 (en) | 2010-10-08 | 2017-12-19 | The University Of North Carolina At Charlotte | Compositions comprising chimeric antigen receptors, T cells comprising the same, and methods of using the same |
| CN103328632A (zh) | 2010-11-30 | 2013-09-25 | 中外制药株式会社 | 与多分子的抗原重复结合的抗原结合分子 |
| AU2012311443B2 (en) | 2011-09-23 | 2016-12-01 | Ablynx Nv | Prolonged inhibition of interleukin-6 mediated signaling |
| ES2819075T3 (es) | 2011-10-11 | 2021-04-14 | Viela Bio Inc | Matrices de soporte derivadas de TN3 específicas para CD40L y sus métodos de empleo |
| CN113416256A (zh) | 2011-11-30 | 2021-09-21 | 中外制药株式会社 | 包含进入细胞内以形成免疫复合体的搬运体(载体)的药物 |
| KR20140132748A (ko) | 2012-03-02 | 2014-11-18 | 아블린쓰 엔.브이. | 슈도모나스 애루기노사 pcrv 결합 단일 가변 도메인 항체 |
| WO2014087010A1 (en) | 2012-12-07 | 2014-06-12 | Ablynx N.V. | IMPROVED POLYPEPTIDES DIRECTED AGAINST IgE |
| US10309969B2 (en) * | 2013-01-24 | 2019-06-04 | Abtlas Co., Ltd. | Protein combination-based FV library, and preparation method therefor |
| EP2989202B1 (de) | 2013-04-25 | 2019-04-10 | Sutro Biopharma, Inc. | Die verwendung des lambda-gam proteins in der ribosomen-display-technologie |
| EP2989239B1 (de) * | 2013-04-25 | 2018-07-04 | Sutro Biopharma, Inc. | Auswahl von fab-fragmenten anhand von ribosomaler display-technik |
| JP6442404B2 (ja) | 2013-06-11 | 2018-12-19 | 国立研究開発法人国立精神・神経医療研究センター | 再発寛解型多発性硬化症（ｒｒｍｓ）患者の治療予後予測方法、及び新規治療適応判断方法 |
| US9409139B2 (en) | 2013-08-05 | 2016-08-09 | Twist Bioscience Corporation | De novo synthesized gene libraries |
| US11554181B2 (en) | 2014-09-05 | 2023-01-17 | The University Of North Carolina At Charlotte | Tumor specific antibody conjugates and uses therefor |
| JP2017534617A (ja) | 2014-10-21 | 2017-11-24 | アブリンクス エン．ヴェー． | Ｉｌ−６ｒ関連疾患の処置 |
| CA2975852A1 (en) | 2015-02-04 | 2016-08-11 | Twist Bioscience Corporation | Methods and devices for de novo oligonucleic acid assembly |
| SG11201705093UA (en) | 2015-02-27 | 2017-07-28 | Chugai Pharmaceutical Co Ltd | Composition for treating il-6-related diseases |
| KR20240093725A (ko) | 2015-03-12 | 2024-06-24 | 메디뮨 엘엘씨 | 알부민 융합 단백질의 정제 방법 |
| WO2016172377A1 (en) | 2015-04-21 | 2016-10-27 | Twist Bioscience Corporation | Devices and methods for oligonucleic acid library synthesis |
| PL3299810T3 (pl) | 2015-05-19 | 2021-12-13 | National Center Of Neurology And Psychiatry | Sposób określania zastosowania nowej terapii u pacjentów ze stwardnieniem rozsianym (sm) |
| ES2881683T3 (es) | 2015-05-21 | 2021-11-30 | Full Spectrum Genetics Inc | Procedimiento para mejorar las características de las proteínas |
| CA2998169A1 (en) * | 2015-09-18 | 2017-03-23 | Twist Bioscience Corporation | Oligonucleic acid variant libraries and synthesis thereof |
| CN108698012A (zh) | 2015-09-22 | 2018-10-23 | 特韦斯特生物科学公司 | 用于核酸合成的柔性基底 |
| CN108603307A (zh) | 2015-12-01 | 2018-09-28 | 特韦斯特生物科学公司 | 功能化表面及其制备 |
| USD782516S1 (en) | 2016-01-19 | 2017-03-28 | Apple Inc. | Display screen or portion thereof with graphical user interface |
| EP3500672A4 (de) | 2016-08-22 | 2020-05-20 | Twist Bioscience Corporation | De-novo-synthetisierte nukleinsäure-bibliotheken |
| JP6871364B2 (ja) | 2016-09-21 | 2021-05-12 | ツイスト バイオサイエンス コーポレーション | 核酸に基づくデータ保存 |
| CA3047128A1 (en) | 2016-12-16 | 2018-06-21 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| CA3054303A1 (en) | 2017-02-22 | 2018-08-30 | Twist Bioscience Corporation | Nucleic acid based data storage |
| WO2018170169A1 (en) | 2017-03-15 | 2018-09-20 | Twist Bioscience Corporation | Variant libraries of the immunological synapse and synthesis thereof |
| US11851486B2 (en) | 2017-05-02 | 2023-12-26 | National Center Of Neurology And Psychiatry | Method for predicting and evaluating therapeutic effect in diseases related to IL-6 and neutrophils |
| WO2018231872A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| WO2018231864A1 (en) | 2017-06-12 | 2018-12-20 | Twist Bioscience Corporation | Methods for seamless nucleic acid assembly |
| WO2019051501A1 (en) | 2017-09-11 | 2019-03-14 | Twist Bioscience Corporation | PROTEINS BINDING TO GPCR AND METHODS OF SYNTHESIS |
| EP3698808A4 (de) | 2017-10-20 | 2021-07-14 | Hyogo College Of Medicine | Anti-il-6 rezeptorantikörper enthaltende medizinische zusammensetzung zur verhinderung von postoperativer adhäsion |
| KR102637566B1 (ko) | 2017-10-20 | 2024-02-16 | 트위스트 바이오사이언스 코포레이션 | 폴리뉴클레오타이드 합성을 위한 가열된 나노웰 |
| US10936953B2 (en) | 2018-01-04 | 2021-03-02 | Twist Bioscience Corporation | DNA-based digital information storage with sidewall electrodes |
| KR20210013128A (ko) | 2018-05-18 | 2021-02-03 | 트위스트 바이오사이언스 코포레이션 | 핵산 하이브리드화를 위한 폴리뉴클레오타이드, 시약 및 방법 |
| AU2020229349A1 (en) | 2019-02-26 | 2021-10-14 | Twist Bioscience Corporation | Variant nucleic acid libraries for GLP1 receptor |
| CA3131691A1 (en) | 2019-02-26 | 2020-09-03 | Twist Bioscience Corporation | Variant nucleic acid libraries for antibody optimization |
| EP3987019A4 (de) | 2019-06-21 | 2023-04-19 | Twist Bioscience Corporation | Barcode-basierte nukleinsäuresequenzanordnung |
| EP4034566A4 (de) | 2019-09-23 | 2024-01-24 | Twist Bioscience Corporation | Variante nukleinsäurebibliotheken für crth2 |
Family Cites Families (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES2078518T3 (es) | 1990-04-05 | 1995-12-16 | Roberto Crea | Mutagenesis por desplazamiento completo. |
| EP1230269A2 (de) | 1999-11-03 | 2002-08-14 | Maxygen, Inc. | Erzeugung von antikörperdiversität |
| AU784983B2 (en) * | 1999-12-15 | 2006-08-17 | Genentech Inc. | Shotgun scanning, a combinatorial method for mapping functional protein epitopes |
| US7647184B2 (en) | 2001-08-27 | 2010-01-12 | Hanall Pharmaceuticals, Co. Ltd | High throughput directed evolution by rational mutagenesis |
| ES2328345T3 (es) * | 2002-04-17 | 2009-11-12 | Bioren, Inc. | Bibliotecas universales para inmunoglobulinas. |
| ATE374255T1 (de) * | 2002-04-17 | 2007-10-15 | Bioren Inc | Doping bei der walk-through-mutagenese |
| KR20060034650A (ko) * | 2003-06-27 | 2006-04-24 | 바이오렌 인코포레이티드 | 룩-스루 돌연변이 |
-
2005
- 2005-07-06 ES ES05809866T patent/ES2372503T3/es active Active
- 2005-07-06 JP JP2007520506A patent/JP4939410B2/ja active Active
- 2005-07-06 CA CA2572917A patent/CA2572917C/en active Active
- 2005-07-06 WO PCT/US2005/024140 patent/WO2006023144A2/en active Application Filing
- 2005-07-06 US US11/571,088 patent/US9012369B2/en active Active
- 2005-07-06 BR BRPI0513155-3A patent/BRPI0513155B1/pt active IP Right Grant
- 2005-07-06 EP EP05809866A patent/EP1774019B1/de active Active
- 2005-07-06 AT AT05809866T patent/ATE528396T1/de not_active IP Right Cessation
- 2005-07-06 MX MX2007000105A patent/MX2007000105A/es active IP Right Grant
-
2011
- 2011-09-15 JP JP2011201518A patent/JP2012041347A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP1774019A2 (de) | 2007-04-18 |
| ES2372503T3 (es) | 2012-01-20 |
| CA2572917C (en) | 2012-04-03 |
| BRPI0513155B1 (pt) | 2021-07-20 |
| US9012369B2 (en) | 2015-04-21 |
| CA2572917A1 (en) | 2006-03-02 |
| BRPI0513155A (pt) | 2008-04-29 |
| JP2012041347A (ja) | 2012-03-01 |
| US20080214406A1 (en) | 2008-09-04 |
| JP2008505642A (ja) | 2008-02-28 |
| EP1774019B1 (de) | 2011-10-12 |
| JP4939410B2 (ja) | 2012-05-23 |
| WO2006023144A2 (en) | 2006-03-02 |
| EP1774019A4 (de) | 2009-05-20 |
| WO2006023144A3 (en) | 2006-08-17 |
| MX2007000105A (es) | 2007-07-18 |
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