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MX2018014993A - Uso de endonucleasa cpf1 para modificaciones de genoma de planta. - Google Patents

Uso de endonucleasa cpf1 para modificaciones de genoma de planta.

Info

Publication number
MX2018014993A
MX2018014993A MX2018014993A MX2018014993A MX2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A MX 2018014993 A MX2018014993 A MX 2018014993A
Authority
MX
Mexico
Prior art keywords
plant
genome
cpf1
cpf1 endonuclease
plant genome
Prior art date
Application number
MX2018014993A
Other languages
English (en)
Inventor
Mark Cigan Andrew
K Young Joshua
DJUKANOVIC Vesna
Original Assignee
Pioneer Hi Bred Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int filed Critical Pioneer Hi Bred Int
Publication of MX2018014993A publication Critical patent/MX2018014993A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

Se proporcionan composiciones y métodos para la modificación gentotica de una secuencia diana en el genoma de una planta o célula vegetal, para la edición genonica de una secuencia de nucleótldos en el gen", de una planta o célula vegetal, y/o para Insertar o delecionar un polinucleótido de interés en o procedente del gano= da una planta. Loa métodos y composiciones emplean un sistema de polinucleótido de gulatandonuclease Cpfl para proporcionar un sistema eficaz para modificar o alterar sitios diana dentro del genes de una planta, célula vegetal o semilla. Además se proporcionan complejos de polinucleótidos de quia/endonucleasas Cpfl, polinucleotidos de quia, secuencias de endonucleaaa Cpfl optimizadas para plantas, construcciones de ADN reconbinante quo comprenden genes da endonuclossaa Cpfl optimizados para plantas, y combinaciones de los mismos.
MX2018014993A 2016-06-14 2017-05-31 Uso de endonucleasa cpf1 para modificaciones de genoma de planta. MX2018014993A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201662349826P 2016-06-14 2016-06-14
US201762468013P 2017-03-07 2017-03-07
PCT/US2017/035175 WO2017218185A1 (en) 2016-06-14 2017-05-31 Use of cpf1 endonuclease for plant genome modifications

Publications (1)

Publication Number Publication Date
MX2018014993A true MX2018014993A (es) 2019-09-06

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
MX2018014993A MX2018014993A (es) 2016-06-14 2017-05-31 Uso de endonucleasa cpf1 para modificaciones de genoma de planta.

Country Status (11)

Country Link
US (1) US20200332305A1 (es)
EP (1) EP3469077A1 (es)
KR (1) KR20190016970A (es)
CN (1) CN109312317A (es)
AU (1) AU2017286122A1 (es)
BR (1) BR112018076027A2 (es)
CA (1) CA3022854A1 (es)
IL (1) IL262795A (es)
MX (1) MX2018014993A (es)
WO (1) WO2017218185A1 (es)
ZA (1) ZA201807366B (es)

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Also Published As

Publication number Publication date
CA3022854A1 (en) 2017-12-21
WO2017218185A1 (en) 2017-12-21
ZA201807366B (en) 2019-06-26
AU2017286122A1 (en) 2018-11-22
BR112018076027A2 (pt) 2019-03-26
IL262795A (en) 2018-12-31
US20200332305A1 (en) 2020-10-22
CN109312317A (zh) 2019-02-05
KR20190016970A (ko) 2019-02-19
EP3469077A1 (en) 2019-04-17

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