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MX2023001088A - Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. - Google Patents

Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.

Info

Publication number
MX2023001088A
MX2023001088A MX2023001088A MX2023001088A MX2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A MX 2023001088 A MX2023001088 A MX 2023001088A
Authority
MX
Mexico
Prior art keywords
methods
making
cell transcriptome
throughput single
nucleic acids
Prior art date
Application number
MX2023001088A
Other languages
English (en)
Inventor
Frank J Steemers
Jay Shendure
Junyue Cao
Molly Gasperini
Jacob Tome
Original Assignee
Illumina Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Illumina Inc filed Critical Illumina Inc
Publication of MX2023001088A publication Critical patent/MX2023001088A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Analytical Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Se proporciona en la presente métodos para preparar una biblioteca de secuenciación que incluye ácidos nucleicos de una pluralidad de células individuales. En una modalidad, la biblioteca de secuenciación incluye ácidos nucleicos que representan los transcriptomas completos de la pluralidad de células individuales. En una modalidad, los ácidos nucleicos incluyen tres secuencias de índice. También se proporcionan en la presente composiciones, tal como composiciones que incluyen los ácidos nucleicos que tienen tres secuencias de índice.
MX2023001088A 2018-06-04 2019-12-16 Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso. MX2023001088A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201862680259P 2018-06-04 2018-06-04
US201962821678P 2019-03-21 2019-03-21

Publications (1)

Publication Number Publication Date
MX2023001088A true MX2023001088A (es) 2023-03-16

Family

ID=68769215

Family Applications (2)

Application Number Title Priority Date Filing Date
MX2019015262A MX2019015262A (es) 2018-06-04 2019-06-04 Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.
MX2023001088A MX2023001088A (es) 2018-06-04 2019-12-16 Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
MX2019015262A MX2019015262A (es) 2018-06-04 2019-06-04 Bibliotecas de transcriptomas de celulas individuales de alto rendimiento y metodos de fabricacion y uso.

Country Status (16)

Country Link
US (1) US20210102194A1 (es)
EP (2) EP4269618A3 (es)
JP (2) JP6986103B2 (es)
KR (2) KR102393414B1 (es)
CN (1) CN111247248A (es)
AU (2) AU2019282158B2 (es)
BR (1) BR112019027750A2 (es)
CA (1) CA3072273A1 (es)
DK (1) DK3810774T3 (es)
ES (1) ES2966028T3 (es)
FI (1) FI3810774T3 (es)
IL (2) IL314667A (es)
MX (2) MX2019015262A (es)
NZ (1) NZ760374A (es)
SG (1) SG11202000905PA (es)
WO (1) WO2019236599A2 (es)

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Also Published As

Publication number Publication date
IL314667A (en) 2024-10-01
KR20220057665A (ko) 2022-05-09
RU2020102911A (ru) 2021-07-27
FI3810774T3 (fi) 2023-12-11
RU2020102911A3 (es) 2021-08-27
US20210102194A1 (en) 2021-04-08
KR20200035955A (ko) 2020-04-06
EP3810774A2 (en) 2021-04-28
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