Papers by Sueli C M Mello
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The objective of this work was to evaluate Trichoderma harzianum isolates for biological control ... more The objective of this work was to evaluate Trichoderma harzianum isolates for biological control of white mold in common bean (Phaseolus vulgaris). Five isolates were evaluated for biocontrol of white mold in 'Perola' common bean under field conditions, in the 2009 and 2010 crop seasons. A commercial isolate (1306) and a control treatment were included. Foliar applications at 2x109 conidia mL‐1 were performed at 42 and 52 days after sowing (DAS), in 2009, and at 52 DAS in 2010. The CEN287, CEN316, and 1306 isolates decreased the number of Sclerotinia sclerotiorum apothecia per square meter in comparison to the control, in both crop seasons. CEN287, CEN316, and 1306 decreased white mold severity during the experimental period, when compared to the control.
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REVISTA AGRO@MBIENTE ON-LINE, 2013
Os fungicidas sintéticos embora sejam eficientes no controle de fungo fitopatogênicos, apresentam... more Os fungicidas sintéticos embora sejam eficientes no controle de fungo fitopatogênicos, apresentam potencial efeito tóxico à saúde humana e ao meio ambiente. Nesse contexto, o controle biológico tem sido utilizado como alternativa para reduzir os prejuízos causados por Sclerotium rolfsii em diversas culturas de importância econômica no Brasil. Sendo assim, objetivou-se com este trabalho estudar o potencial de 23 isolados de Trichoderma harzianum provenientes de solo de Cerrado como agente de controle biológico de S. rolfsii, importante patógeno nas culturas do feijoeiro e soja. A ação antagonista dos isolados foi verificada por meio do pareamento de culturas sob três temperaturas (22ºC, 25ºC e 28ºC) e em experimentos conduzidos em casa de vegetação, com as culturas de feijoeiro e soja. Os isolados CEN155, CEN158, CEN169, CEN170, CEN194 e CEN197 destacaram-se quanto à inibição do crescimento micelial de S. rolfsii, nas três temperaturas empregadas nos experimentos in vitro, ocupando p...
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Biologia
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The Plant Pathology Journal
Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. ... more Common bean (Phaseolus vulgaris L.) is one of the most important crops in human food production. The occurrence of diseases, such as white mold, caused by Sclerotinia sclerotiorum can limit the production of this legume. The use of Trichoderma has become an important strategy in the suppression of this disease. The aim of the present study was to evaluate the effect of volatile organic compounds (VOCs) emitted by Trichoderma azevedoi CEN1241 in five different growth periods on the severity of white mold in common bean. The in vitro assays were carried out in double-plate and split-plate, and the in vivo assays, through the exposure of the mycelia of S. sclerotiorum to the VOCs of T. azevedoi CEN1241 and subsequent inoculation in bean plants. Chemical analysis by gas chromatography coupled to mass spectrometry detected 37 VOCs produced by T. azevedoi CEN1241, covering six major chemical classes. The profile of VOCs produced by T. azevedoi CEN1241 varied according to colony age and wa...
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Revista Brasileira de Biociências, 2016
Fungi of the Trichoderma genus have a versatility of action, as they can act by direct and/or ind... more Fungi of the Trichoderma genus have a versatility of action, as they can act by direct and/or indirect mechanisms. In view of this, multivariate analysis has been used in order to summarize and identify correlations among different mechanisms of biological control. We aimed to evaluate the antagonism of 16 Trichoderma spp. isolates against Sclerotinia sclerotiorum and identify the variables that best explain the antagonistic activity of Trichoderma spp. isolates against the pathogen in vitro. For that, isolates were confronted with the pathogen in paired culture (PC) and had the effect of their metabolites on the pathogen growth evaluated in volatile (VM), thermolabile (TLM), and non-thermolabile metabolite (NTLM) assays. Isolate CEN654 allowed only 17.1, 9.5, and 0.3% of pathogen growth in relation to the control in the VM, TLM, and NTLM assays respectively, standing out from the other isolates in the principal component analysis (PCA). The PCA also suggested that the PC and TLM as...
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The objective of this research is to characterize and discover lineages with higher parasitic act... more The objective of this research is to characterize and discover lineages with higher parasitic activity with good adaptation to the different enviroments where these agents will be used.
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Journal of Scientific Research and Reports, 2022
Fungi of the Trichoderma genus are present in practically all types of soil and they have the abi... more Fungi of the Trichoderma genus are present in practically all types of soil and they have the ability to establish a beneficial relationship with plants. In addition to acting as direct biological control agents, they also act as plant growth promoters, by an indirect biological control mechanism. Because of these, many products containing Trichoderma strains are used to improve seed health, providing better development of roots and aerial parts of plants. In view of this fact, research work was carried out in a greenhouse with the aim of evaluating the effect of five Trichoderma strains, belonging to the species T. virens, T. asperellum, T. asperelloides, in addition to a strain unidentified at the species level T. koningiopsis, in three new crisp lettuce cultivars (BRS Lélia, BRS Leila, and BRS Mediterrânea). A conidial suspension of each of the strains was prepared (1.0 x 107 conidia mL-1) and applied at the time of sowing the lettuce in pots. The experiment was completely random...
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Journal of Agricultural Science, Jul 10, 2018
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Revista Árvore, 2018
Teak is a forest species that has assumed great importance in Brazil, where it has found excellen... more Teak is a forest species that has assumed great importance in Brazil, where it has found excellent conditions for development since its introduction into the country in the 1960s. However, phytosanitary problems are beginning to threaten the production of this timber species. An example is teak canker, caused by the fungus Lasiodiplodia theobromae (Lt), which has only recently been reported in Brazil, and for which, therefore, there are no recommended control methods. Thus, this study evaluated the control of this pathogen, investigating the potential of the biocontrol agents (BCAs) Trichoderma spp., Bacillus sp. and Enterobacter sp., initially through in vitro assays and, subsequently, with in vivo tests. According to the in vitro assay results, the Trichoderma isolates CEN162 and CEN1153 and the strain of Bacillus sp. (UnB1366) were the treatments that stood out, as they were able to completely inhibit mycelial growth of some isolates of Lt. When these isolates were tested in a pr...
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PLOS ONE, 2020
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JOURNAL OF NEOTROPICAL AGRICULTURE, 2018
Alguns fungos apresentam difícil esporulação em meios artificiais. Neste contexto, o processo de ... more Alguns fungos apresentam difícil esporulação em meios artificiais. Neste contexto, o processo de produção massal de conídios de Trichoderma sp., para o emprego em biocontrole mais utilizado, se baseia na multiplicação por meio da fermentação em substrato sólido. O objetivo deste trabalho foi estudar a produção de conídios de cinco isolados de T. harzianum em substrato sólido e a capacidade destes para colonização superficial de meio de cultura rico em sacarose. Para tanto, os isolados foram submetidos à produção de conídios em substrato sólido (arroz parboilizado umedecido e autoclavado) a 25 ºC e a fotoperíodo de 12h por sete dias. Em seguida, os conídios foram inoculados em quadrados (1,5 x 1,5 cm) de batata dextrose ágar-sacarose (BDA-S) para se estimar o percentual de colonização dos quadrados às 12, 24, 36 e 48 horas após a inoculação a 25 ºC e fotoperíodo de 12 h. O percentual de colonização foi integralizado como AACCM (área abaixo da curva de crescimento micelial). Os isolad...
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Pesquisa Agropecuária Brasileira, 2015
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Summa Phytopatologica, 2005
Isolates of Cercospora caricis that incite leafspot of purple nutsedge (Cyperus rotundas) were ev... more Isolates of Cercospora caricis that incite leafspot of purple nutsedge (Cyperus rotundas) were evaluated on the mycelial growth and sporulation in different conditions of culturing. In general, in tests using CEN 051 and CEN 055 isolates alone, the isolated CEN 055 showed greater mycelial growth than CEN 051. However, the isolate CEN 051 showed more sporulation capacity. The best medium for culturing the isolate CEN 055 were: purple nutsedge leaf-extract agar, carrot root-extract agar, potato-dextrose agar, sugar cane molasses agar, nutsedge leaf-juice agar, and the synthetic CSM. A seven day culturing period was suitable for observing the occurrence of sporulation in studies where 10 isolates were tested, despite the low spore density verified. In another study, 13 isolates were tested for sporulation capacity using nutsedge leaf fragments in water-agar media. Sporulation was observed on all isolates, despite of their differences regarding their responses to light.
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Fitopatologia Brasileira, 2001
Isolados de Alternaria spp. patogênicos ao fedegoso (Senna obtusifolia) foram estudados quanto ao... more Isolados de Alternaria spp. patogênicos ao fedegoso (Senna obtusifolia) foram estudados quanto aos aspectos morfológicos e padrões isoenzimáticos, em gel de poliacrilamida. Com base nas características culturais, morfologia de colônias e morfometria de conídios, verificou-se que 11 dos 13 isolados pertenciam à espécie A. cassiae e os outros dois à A. alternata. Os resultados da análise eletroforética corroboraram as informações obtidas por meio de critérios morfológicos. Portanto, esta técnica tem potencial para ser usada na separação destas espécies de Alternaria. A ocorrência de mais de uma espécie deste gênero fúngico em S. obtusifolia foi constatada, ampliando o número de patógenos a serem avaliados no programa de desenvolvimento de um bioherbicida para esta espécie de planta daninha.
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Pesquisa Agropecuária Brasileira, 2000
Visando ajustar metodologia de produção de inóculo, um isolado do fungo Alternaria cassiae, selec... more Visando ajustar metodologia de produção de inóculo, um isolado do fungo Alternaria cassiae, selecionado como agente para biocontrole de fedegoso (Senna obtusifolia), foi estudado quanto ao crescimento micelial e esporulação em diferentes meios de cultura e condições de cultivo. Meio V-8 caseiro sem adição de CaCO3, incubação em volume de 200-250 mL de meio (cerca de 50% do volume do frasco), período de 48 horas de agitação a 250 rpm, seguida de exposição da massa micelial em escuro contínuo foram as condições que se mostraram mais adequadas para a produção de inóculo em larga escala. A esporulação foi favorecida por incubação da massa micelial a 25ºC. A adoção de 300 mL de massa fúngica por bandeja (29,9 x 44,5 x 5,0 cm) possibilitou secagem rápida, assegurando a integridade dos conídios, refletida na maior infectividade do inóculo produzido.
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Pesquisa Agropecuária Brasileira, 2008
O objetivo deste trabalho foi avaliar quatro isolados do fungo antagonista Dicyma pulvinata quant... more O objetivo deste trabalho foi avaliar quatro isolados do fungo antagonista Dicyma pulvinata quanto à compatibilidade com defensivos agrícolas e adjuvantes, e a eficiência desses isolados no controle do mal-das-folhas (Microcyclus ulei) da seringueira (Hevea sp.) em campo. A compatibilidade dos isolados com defensivos e adjuvantes foi avaliada com base no crescimento micelial e na esporulação do fungo, em testes conduzidos in vitro. Para avaliação da eficiência do antagonista contra M. ulei, em campo, os quatros isolados do antagonista foram comparados ao controle exercido pelo tratamento com os fungicidas propiconazol + mancozeb. Os ensaios conduzidos em laboratório indicaram a incompatibilidade de D. pulvinata com os fungicidas benomyl, carbendazim, mancozeb, propiconazol e, ainda, com o inseticida acaricida endosulfan. Dois dos adjuvantes testados (Tween 20 e Tween 80) não afetaram o desenvolvimento do fungo. Foi comprovada a ação do antagonista D. pulvinata sobre M. ulei, em camp...
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Papers by Sueli C M Mello