Leica M125 C Leica M165 C Leica M205 C Leica M205 A User Manual

Leica M125 C
Leica M165 C
Leica M205 C
Leica M205 A
User Manual
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General Instructions
Safety concept Use in clean rooms Servicing

Before using your microscope for the first The Leica M series can be used in clean rooms OO Repairs may only be carried out by Leica
time, please read the "Safety concept" booklet without any problems. Microsystems-trained service technicians.
included with your instrument. It contains addi- Only original Leica Microsystems spare
tional information about handling and care. Cleaning parts may be used.
OO Do not use any unsuitable cleaning agents,
chemicals or techniques for cleaning.
Responsibilities of person in charge of
OO Never use chemicals to clean colored instrument
surfaces or accessories with rubberized OO Ensure that the Leica stereo microscope
parts. This could damage the surfaces, is operated, maintained and repaired by
and specimens could be contaminated by authorized and trained personnel only.
abraded particles.
OO In most cases, we can provide special solu-

tions on request. Some products can be
modified, and we can offer other accesso-
ries for use in clean rooms.
Leica M Series User Manual 2

Safety Concept
The individual modules of the Leica M stereo You can combine individual system articles
microscopy series include an interactive with articles from external suppliers (e.g. cold
CD-ROM with all relevant user manuals in 20 light sources, etc.). Please read the user manual
other languages. Keep it in a safe place, and and the safety requirements of the supplier.
readily accessible to the user. User manu-
als and updates are also available for you Before installing, operating or using the instru-
to download and print from our web site at ments, read the user manuals listed above. In
www.stereomicroscopy.com. particular, please observe all safety instructions.
This operating manual describes the special To maintain the unit in its original condition
functions of the individual modules of the Leica and to ensure safe operation, the user must
M stereo microscopy series and contains impor- follow the instructions and warnings contained
tant instructions for their operational safety, in these user manuals.
maintenance, and accessories.
The "Safety concept" booklet contains addi-

tional safety information regarding the service
work, requirements and the handling of stereo
microscope, accessories and electrical accesso-
ries as well as general safety instructions.

Symbols Used
Read and understand user manual Danger due to hot surface

before using this device. This symbol warns against touching hot
surfaces, e.g. those of light bulbs.
Warning of a danger
•
This symbol indicates especially impor- Important information
tant information that must be read and This symbol indicates additional infor-
complied with. Failure to comply can cause the mation or explanations that intend to
following: provide clarity.
OO Hazards to personnel
OO Functional disturbances or damaged Action
OO This symbol refers to actions described in
instruments
the text that are to be carried out.
Warning of hazardous electrical voltage

This symbol indicates especially impor- Figures
tant information that, if not observed, (1) Numbers in brackets within the descrip-
can cause the following: tions relate to the figures and the items
within those figures.
OO Hazards to personnel
OO Functional disturbances or damaged
instruments

Safety Instructions
Description Unauthorized alterations to the instrument or Responsibilities of person in charge of

The individual modules fulfill the highest noncompliant use shall void all rights to any instrument
requirements for observation and documenta- warranty claims. OO Refer to "Safety Concept" booklet
tion of Leica stereo microscopes of the M series.
Place of use Ensure that:
Intended use OO Refer to "Safety Concept" booklet OO The M series stereo microscopes and
OO Refer to "Safety Concept" booklet accessories are operated, maintained and
OO Electrical components must be placed at repaired by authorized and trained person-
least 10 cm away from the wall and from nel only.
Non-intended use flammable substances.
OO Refer to "Safety Concept" booklet OO All operators have read, understood and
OO Avoid large temperature fluctuations, observe this User Manual, and particularly
Never use M series microscopes or their compo- direct sunlight and vibrations. These condi- the safety instructions.
nents for surgical procedures (e.g. on the eye) tions can distort measurements and micro-
unless they are specifically intended for that graphic images.
purpose.
OO In warm and warm-damp climatic zones,
The instruments and accessories described in the individual components require special
this operating manual have been tested for care in order to prevent the build-up of
safety and potential hazards. The responsible fungus.
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
are outside of the scope of this manual.

Safety Instructions (Continued)
Repairs, service work Integration in third-party products Health risks
•
OO Refer to "Safety Concept" booklet OO Refer to "Safety Concept" booklet Workplaces with stereo microscopes
facilitate and improve the viewing task,
OO Only original Leica Microsystems spare but they also impose high demands on the eyes
parts may be used. Disposal and holding muscles of the user. Depending on
OO Refer to "Safety Concept" booklet the duration of uninterrupted work, asthenopia
OO Before opening the instruments, switch off and musculoskeletal problems may occur. For
the power and unplug the power cable. this reason, appropriate measures for reduction
Legal requirements of the workload must be taken:
Touching the live circuit can cause OO Refer to "Safety Concept" booklet
injury. OO Optimal arrangement of workplace, work
assignments and work flow (changing tasks
EC Declaration of Conformity frequently).
Transport OO Refer to "Safety Concept" booklet
OO Use the original packaging for shipping or OO Thorough training of the personnel, giving
transporting the individual modules of the consideration to ergonomic and organiza-
Leica M stereo microscopy series and the tional aspects.
accessory components.
OO The ergonomic design and construction of
OO In order to prevent damage from vibrations, the Leica M stereo microscopy series are
disassemble all moving parts that (accord- intended to reduce the exertion of the user
ing to the user manual) can be assembled to a minimum.
and disassembled by the customer and
pack them separately.

• potential
Direct contact with eyepieces is a Instrument safety and EMC The device meets the requirements of EU
transmission method for Our device has been designed, produced and directives
bacterial and viral infections of the eye. tested in compliance with
OO 2014/35/EU Low voltage directive
The risk can be kept to a minimum by using OO IEC EN 61010-1: Electrical equipment for OO 2014/30/EU EMC directive
personal eyepieces for each individual or measurement, control and laboratory use
– Safety – Part one: general requirements OO 2011/65/EU RoHS directive
detachable eyecups.
OO EN 60950-1: Information technology and carries the CE mark.
Technical safety equipment – Safety – Part one: general

To connect the Leica M Series microscopes to requirements
power, use a power cable that meets the power The device must be disposed of in
cable specifications in your country. The power OO Radio interference suppression in compli- accordance with 2012/19/EU WEEE
socket used has to be equipped with a 16 A or ance with EN 55011 class B directive.
10 A fuse. Only connect the instrument to a
grounded power socket that has been installed OO Noise immunity in compliance with
according to regulations. The power supply DIN EN 61326-1. Intended for indoor use only in all EU member
voltage has to correspond to the voltage speci- states, EFTA states and Switzerland.
fied on the identification label. Make sure that
the ground connection is neither defective
nor interrupted, since otherwise a malfunction
poses a risk of fatal injury. Never unplug the
power plug with moist or wet hands. There is
danger of an electric shock.

Handling electrical components

Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The microscope must be connected to a

grounded outlet.
Always position the microscope so that

you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.

Contents
General Instructions 2 Leica IsoPro Manual XY Stage: Assembly 31

Safety Concept 3 Cables: Connections 34
Symbols Used 4 Cables: Cable Duct 35
Safety Instructions 5 Cables: Diagram 36
Congratulations!11 Leica LED5000 MCI 37
A Step Towards Infinity 12 Leica LED5000 MCI: Alternative Assembly 38
The Electronics: Comfort, Convenience and Safety for Your Experiments13 Leica LED5000 RL: Assembly 39
The Modular Design: Everything is Relative 14 Leica LED5000 RL: Installing Optional Accessories 41
Maximum Compatibility 15 Leica LED5000 RL: Use 42
On We Go 16 Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes
(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) 44
Assembly Leica LED5000 NVI: Installing Optional Accessories 47
Base and Focusing Column 18
Assembling the Focusing Column for TL Bases 19 Quick Start Guide
Assembling the Focusing Column With an Incident-light Base 20 The Fastest Route to Success 50
Optics Carrier 21 Overview of an M Series Microscope 51
Tube22 The Correct Interpupillary Distance 52
Eyepieces23 Using the Eyepieces 53
Objective24 Focusing54
Objective Nosepiece – Assembly 25 Adjusting the Resistance of the Focus Drive 55
Objective Nosepiece – Adjusting Parfocality 26 Changing the Magnification (Zoom) 56
AX Carrier – Preparation 27 Ratchet Steps and Magnification Levels 57
AX Carrier – Assembly 28 Parfocality: More Comfort and Convenience for Your Work 58
Transmitted-light Base Leica TL BFDF: Before First Use 29 Iris Diaphragm 59
Transmitted-light Base Leica TL BFDF 30

Contents (Continued)
Eyepieces System Illumination

Magnification Factors of the Eyepieces 61 Leica LED5000 MCI 83
Health Notes 62 Leica LED5000 RL 85
Dioptric Correction 63
Dioptric Correction and Parfocality 64 Accessories
Graticules65 Leica Hand Wheel and Foot Switch 87
Leica SmartTouch 88
Photography & Video
Photography & Video 67 Dimensional Drawings
Photo Tubes and C-mounts 68 Leica M125 C / Leica M165 C 90
Trinocular Video/Phototube 50 % 69 Leica LED5000 MCI 92
Trinocular Video/Phototube 100 % 70 Leica LED5000 RL 93
Trinocular Video/Phototube 100 %: ErgoTube 5°– 45° 71 Leica LED5000 NVI 94
Leica MATS TPX 95
Microscope Carrier
The Microscope Carrier AX 73 Specifications
The Objective Nosepiece 75 Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A 97
Transmitted-light Base Leica TL BFDF 101
Objectives and Optical Accessories Motorized Focus Drives With "Zoom" Carrier
The Different Types of Objectives 77 (M125 C, M165 C, M205 C, M205 A) 102
Bases Appendix
Transmitted-light Base Leica TL BFDF: Controls 79 Calculating the Total Magnification and Field of View Diameter 104
Transmitted-light Base Leica TL BFDF: Operation 80 Care, Maintenance, Contact Persons 105
Leica IsoPro (Non-motorized): Controls 81

Congratulations!
Congratulations on obtaining your new Leica M series stereo microscope. Though the reliability and robustness of Leica stereo microscopes is
We are convinced it will exceed your expectations, as never before have legendary, like any high-tech product, the M series requires a certain
we applied our decades of experience in the areas of optics, mechanical degree of care and attention. Therefore, we recommend that you read
engineering and ergonomics in such an uncompromising manner. this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
The M series embodies all the qualities you associate with the name Leica that even after years of intensive use, your stereo microscope will continue
Microsystems: excellent objectives, high-quality engineering, and reli- to work as smoothly and reliably as on the very first day.
ability. Furthermore, the modular design ensures that the M series adapts
perfectly to your needs – no matter which accessories you require for your We wish you the best of success in your work – after all, you are now
tasks. equipped with the best tool!

A Step Towards Infinity
Ever since their introduction by Horatio S. Limits are made to be broken.

Greenough, stereo microscopes have worked The Leica M205 C is the world's first stereo
according to the optical principles based microscope with a zoom range of 20.5:1. This
primarily on Ernst Abbe's research. For over a accomplishment, however, was not enough for
century, ingenious optics designers and engi- Leica's engineers. With the new FusionOptic in
neers have worked to push magnification, reso- the M205 C, they have succeeded in going yet
lution and image fidelity to the limit permitted another step beyond previous limits. In addition
by optics. to the increase in magnification, the resolution,
too, has been increased to up to 1,050 lp / mm*,
In doing so, they have always been constrained which corresponds to a resolved structure size
by the interrelation between three factors: the of 472 nm.
higher a microscope's resolution, the lower the
available working distance. If one increases the Of course, this performance increase benefits
distance of the optical axes, the three-dimen- your everyday work. Set up your specimens
sional image seen by the observer becomes on the microscope with comfortable freedom
distorted – a sphere becomes an ellipse, a flat of movement and discover details in stereo
surface curves toward the observer. microscopy that you could never see before.
* Leica M205 C with 1.0× planapochromat and 10× eyepieces

The Electronics: Comfort, Convenience and Safety for Your Experiments
Never before have electronics been used as Reliability for your experiments diaphragm and more to the Leica LAS X soft-
extensively in a Leica series as in the new Exact reproducibility of results is an increas- ware. Thus you always know the conditions
M series. Optics carrier, tripod, base and illumina- ingly common demand, particularly in research under which an image was acquired.
tor are all connected using electrical contacts – and development. The continuous encoding
which provides a number of advantages. captures parameters such as the magnifica- Fewer cables
tion, the illumination, the position of the iris A large part of the cables have been routed in
the interior of the column. Data are also trans-
ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables – this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply Leica Application Suite X (LAS X) evaluates the
the power. transmitted data and can restore the test
situation later.

The Modular Design: Everything is Relative
The Leica M series provides maximum flexibil- Despite this, you will notice that the controls Have a special request? Let us know!
ity in choosing equipment, thanks primarily to and individual components do not differ signif- Leica Microsystems enjoys an exceptional repu-
the modular configuration and the compatibil- icantly. Whichever configuration you choose, tation when it comes to devising customer-
ity that Leica has painstakingly maintained for you will quickly feel right at home. specific solutions. If you have a special request
decades. The optics carriers, eyepieces, bases that cannot be met with standard parts, contact
and more can be combined in any way you your Leica consultant. We have a solution for
choose, allowing you to create the microscope every problem.
that best suits your needs.

Maximum Compatibility
Leica engineers were careful to ensure that the Tubes

new M series – like its predecessors – remains The interface between the optics carrier and
compatible with existing series. This means the tube has remained the same, so exist-
that objectives, bases, tubes and so on can be ing tubes fit the new M series. The new tubes
reused. are designed for eyepieces with field number
23, while the predecessor models were only
Objectives designed for field number 21, resulting in a
All new objectives of the M series are parfocal, smaller object field.
meaning that when used with the objective
nosepiece, they can be replaced while keeping Eyepieces
the specimen in perfect focus. However, the new M-series eyepieces models
have an audible and tangible click to provide
If you prefer, you can continue to use the previ- immediate feedback in case of accidental
ous objective series. In this case, parfocality is adjustment.
no longer guaranteed.

On We Go
If your new Leica microscope has already been If, on the other hand, you are assembling
assembled and commissioned by your Leica the microscope yourself, continue with the
consultant, click here to skip through the instal- "Assembly" chapter, which begins on page 17.
lation instructions and go directly to the Quick
Start Guide on page 49.

Assembly
Leica M Series User Manual Assembly17

Base and Focusing Column
The first step is to connect the focusing column Assembling the column adapter Assembling the focusing drive
of the M series to the corresponding base. 1. Securely install the column adapter on the 2. Securely screw the focusing column to the
column using the four included screws. base using the six included screws.
Tools used
OO Hex socket screwdriver, 3 mm

Assembling the Focusing Column for TL Bases
The first step is to connect the focusing column Assembling the column adapter Assembling the focusing column
of the M series to the corresponding base. 1. Securely install the column adapter on the 2. Securely screw the focusing column to the
column using the four included screws. base using the six included screws.
Tools used

Assembling the Focusing Column With an Incident-light Base
When using an incident-light base, the focus- Assembly 3. Screw the base securely onto the focusing
ing column and motorized focus are installed 1. Place the focusing column on the side. drive.
directly on the base; no extension plate is
required.
Tools used
2. Insert the four screws provided into the

outer holes of the base.

Optics Carrier
Tools used Assembling the optics carrier

OO Hex socket screwdriver, 4 mm 1. Place the optics carrier on the focusing 2. Press the optics carrier backwards to the
column so that the screw fit into the thread focusing column and screw it in place using
provided and the lug fits into the groove. your other hand.
Set the on/off switch to "0" to switch off

the device.

Tube
All intermediate tubes that fit between the Assembling the tube
optics carrier and the binocular tube are fitted 2. Push the tube (for example, the inclined 3. While holding the tube only slightly, care-
in the same manner. binocular tube) into the dovetail ring and fully tighten the positioning screw. It
rotate it slightly in both directions until the is automatically brought to the correct
Tools used positioning screw meshes with the guide position.
OO No tools required. groove.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.

Eyepieces
Tools used Preparation Inserting the eyepieces

OO No tools required. 1. If you want to use an optional graticule, 3. Push the eyepieces into the tubes as far as
insert it now (page 65). they will go and check to ensure that they
Magnification range fit tightly and accurately.
You can extend the overall magnification range 2. Remove the plastic tube guard.
using available 10×, 16×, 25× and 40× wide-
field eyepieces for persons wearing glasses.
4. Securely tighten the clamping screws.

Objective
Tools used Attaching the objective Alternative fastening options
•
OO No tools required. Hold the objective firmly during assem- OO If using the objective nosepiece, read the
bly and disassembly so that it does not instructions on page 25.
fall onto the stage plate. This applies particu-
Preparation larly to the 2× planapochromatic objective, OO If using the AX carrier, read the instructions
1. Remove the protective cap on the optics which is very heavy. Remove all specimens from on page 27.
carrier by turning it. the stage plate first.
2. Screw the objective clockwise into the

optics carrier.

Objective Nosepiece – Assembly
Preparations 2. Rotate the moving part by 90° and attach 4. Screw the optics carrier onto the objective
•
Hold the objectives firmly during the objective nosepiece to the drive hous- nosepiece.
assembly and disassembly so that they ing from the front (!). Screw the objective
do not fall onto the stage plate. nosepiece firmly into place. 5. Screw both objectives onto the objective
nosepiece. It makes no difference which
OO Move the drive housing all the way upwards position an objective occupies.
and remove the optics carrier, if the carrier
has already been installed. 6. Unscrew the locking screws on both sides
of the objective nosepiece.
Assembly
1. Remove the transport anchor from the
objective nosepiece.
3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.
You can now adjust the parfocality (see instruc-

tions on the next page).

Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried Adjustment 7. Toggle to the 1× objective.
out once. Afterwards, both objectives are parfo- 1. Rotate the 2× objective into the beam path
cal, meaning that the specimen remains in and set it to the lowest magnification. 8. Turn the objective on the thread in both
focus when the objective nosepiece is rotated. directions until the specimen appears
2. Focus on the specimen. absolutely sharp.
This procedure must be repeated if you replace
either of the two objectives with another. 3. Rotate the 1× objective into the beam path. By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
The following example assumes the combi- 4. Turn the objective on the thread in both with the other objective. If it is not parfocal,
nation of the 1× and 2× planapochromats. If directions until the specimen appears repeat the procedure.
you are using another objective combination, sharp.
replace the 2× objective in the description with 9. Tighten the locking screws.
the objective with the stronger magnification. 5. Toggle to the 2× objective.
6. Select the strongest magnification and

Preparation refocus until the specimen appears abso-
OO Open the iris diaphragm. lutely sharp.
OO Set the dioptric correction of the eyepieces

to "0".

AX Carrier – Preparation
Tools OO If the switch position corresponds to the

OO Hex socket screwdriver, 2.5 mm optics carrier used, skip the following steps.
Otherwise, proceed as follows:
Preparing the AX carrier 2. Unscrew the two screws that hold the
Before the AX carrier is installed, it may first switch in position.
have to be prepared for the optics carrier to
be used (Leica M125 C, M165 C, and M205 C /
M205 A). 4. Important: push the switch all the way into
the mount while reinserting the screws.
1. Check the switch position. Only this ensures that the switch is posi-
tioned correctly.
3. Pull out the switch, rotate it into the desired

position and reinsert it.

AX Carrier – Assembly
Tools 2. Install the AX carrier on the focusing 4. Unscrew the three holding screws and
OO Hex socket screwdriver, 4 mm column. remove the adapter ring on the optics
OO Philips screwdriver carrier.
Once the AX carrier has prepared for the corre-

sponding optics carrier, it can be installed.
1. Move the slide of the AX carrier into mid

position.
3. Screw the objective to the AX carrier.

5. Install the optics carrier on the AX carrier.
Wrong Right

Transmitted-light Base Leica TL BFDF: Before First Use
Removing the transport anchors
•
Before you can use the transmitted-
light base for the first time, it is abso-
lutely necessary to remove the two transport
anchors as follows. Anchor of mirror
Anchor of switching slide

Transmitted-light Base Leica TL BFDF
Standard delivery The selected stage is mounted on the base Standard stage
The base is delivered with the adapter plate before commissioning. You can switch between 1. Take the glass plate from the rectangular
installed. The selected stage (XY stage or the stages at any time with just a few hand gap in the standard stage.
standard stage 10 450 562), and the focusing movements.
drive will have to be mounted later. 2. Position the stage on the transmitted-light
The following paragraph assumes use of the base in such way that the four holes align
Stage assembly base without the stage mounted. Disassembly over those in the base.
The TL BFDF transmitted-light base can be is performed in reverse order of the following
equipped with different stages. steps. 3. Attach the stage to the base using the four
Allen screws provided.
OO Leica IsoPro manual XY stage
OO Standard stage 10 450 562 4. Insert the glass plate back into the standard
stage.

Leica IsoPro Manual XY Stage: Assembly
IsoPro XY stage Before the IsoPro XY stage is mounted to the Left or right operation
Before the IsoPro XY stage is mounted to the base, the axis containing the control buttons is If the controls are to be mounted on the
base, the axis containing the control buttons is attached either on the left or the right side of left-hand side, the gear rod on the bottom
attached either on the left or the right side of the XY stage. side of the XY stage must be unscrewed and
the XY stage. reattached in reverse.
If the controls are to be mounted on the

left-hand side, the gear rod on the bottom
side of the XY stage must be unscrewed and
reattached in reverse.
1. Take the glass plate from the XY stage.
2. Turn the XY stage around and place it onto

a non-slip surface.
3. Change the gear rod from the left to the

right-hand side.
4. Skip the next two steps to mount the

controls.

Leica IsoPro Manual XY Stage: Assembly (Continued)
1. Take the glass plate from the XY stage and Control assembly XY stage assembly
turn it around. 1. Take the glass plate from the XY stage and 1. Place the XY stage on the base.
turn it around.
2. Change the gear rod from the left to the 2. Pull the upper part of the XY stage carefully
right-hand side. 2. Attach the axis with the control buttons to toward the user, fastening the lower part
the desired side. The fastener snaps into the onto the transmitted-light base.
XY stage magnetically.
4. Attach the axis with the two Allen screws

provided.
5. Attach the cover rail to the XY stage.

Leica IsoPro Manual XY Stage: Assembly (Continued)
3. Screw the XY stage evenly onto the three Focusing drive and column
threaded holes. 1. Unscrew the extension plate from the base
using the Allen key provided.
4. Push the XY stage all the way back towards
the column. 2. Attach your focusing drive column to the
bottom using the three Allen screws.
5. Reinsert the glass plate back into the stan-
dard stage. 3. Reattach the adapter plate to its original
position using the three Allen screws.

Cables: Connections
The new Leica M series features extensive The terminals The connection to the PC and to other instru-
encoding with which various microscope data ments is made using the terminals on the rear
and settings can be read out, transferred to the side of the column:
PC and reproduced later.
1 1. 3×CTL2 terminals for auxiliary equipment
1
OO The interface to the optics carrier is on the 3 such as the ring illuminator, the base and
column. other accessories from the Leica product
range.
2
2. Terminal for the power supply provided.
3. USB terminal for the connection to the PC.

Cables: Cable Duct
The integrated cable duct in the column Feeding the cables 3. Place the cables in the cable duct and screw
enables a neat cable layout around the micro- 1. Unscrew the three screws on the cable the cover on tightly.
scope. For example, the USB or FireWire cables duct.
of the camera can be stowed in the cable duct.
Tip: Estimate the length of the cable ends you

2. Remove the cover of the cable duct. will need before screwing on the cover. For
thick cables, it is difficult to change the length
retroactively.

Cables: Diagram
Alternative installation
LED5000 SLI
Please use the Leica Application
Suite X to configure the functions
of the system.
LED5000 RL LED5000 MCI
PC USB Power/USB 115/230 V
Footswitch(es) Handwheel SmartTouch

Leica LED5000 MCI
The Leica LED5000 MCI (for "Multi Contrast Assembly 2. Connect the CAN-bus cable to either of the
•
Illumination") is installed using two screws. For 1. Hold the LED5000 MCI with one hand two sockets. (The flat part of the plug must
optimum accessibility, the optics carrier should and tightly screw the retraining stirrups be facing downwards.)
be removed during installation (see page 21.) on both top holes on the drive housing.
3. Plug the other end of the cable into one of

the three "CTL2" sockets on the column.
Constraints
The Leica LED5000 MCI cannot be used together
with the objective nosepiece.

Leica LED5000 MCI: Alternative Assembly
Under certain circumstances, the light source 2. Screw the retaining stirrup into the column 3. Push the Leica LED5000 MCI onto the
must not be moved along with the optics at the notch using the single screw. If you retaining stirrup.
carrier. A typical example is multifocus images tighten the screw, the retaining stirrup
in which the Z-stack changes while the angle is automatically moved into the correct
of incidence of the light must remain the same. position.
For such purposes, the Leica LED5000 MCI is
directly fastened to the column.
Installation on the column

1. Pull the retaining stirrup out of the Leica
LED5000 MCI.

Leica LED5000 RL: Assembly
Required tools Assembly 3. Plug the other end of the cable into an
OO None 1. Connect the Leica CAN bus cable provided available CTL2 socket on the column.
to the ring illuminator.
The Leica LED5000 RL is installed on the
objective using a single screw. It has
been optimized for a working distance between
50 mm and 80 mm.
Constraints
The Leica LED5000 RL can be used only in
conjunction with the planapochromat 1× and 2. Place the Leica LED5000 RL against the Continued on next page.
planapochromat 0.63× objectives. With all objective from below, push it up as far as it
other objectives, the working distance is too will go and screw it into place.
low for adequate illumination.
The ring illuminator cannot be used together

with the objective nosepiece.

Leica LED5000 RL: Assembly (Continued)
If you work with the manual focusing For optimum accessibility of the speci-
column without integrated electronics, men, the ring illuminator should be
the Leica LED5000 RL must be supplied with installed with the cable facing backwards.
power via an external power supply unit (not However, it is also possible to turn the ring
included in the delivery package). Moreover, illuminator sideways, for example if simultane-
the illuminator cannot be controlled via Leica ously using the Leica LED5000 MCI system illu-
Application Suite X in this case. minator. In this case, the ring illuminator cannot
be connected directly to the Leica LED5000 MCI.

Leica LED5000 RL: Installing Optional Accessories
Installing optional accessories

OO You can screw the optional polarization
set into place firmly using the metal plate
provided.
OO The optional diffuser is attached and

screwed in below the ring illuminator.
OO The optional polarization set (polarizer and

analyzer) is attached and screwed in below
the ring illuminator.


Leica LED5000 RL: Use
The light of the Leica LED5000 RL can Using the front keypad Using the side keypad
be very bright. Therefore, always switch OO Use the and keys to toggle between
on the illuminator before you look through the the illuminator segments.
eyepieces! Avoid looking directly into the LEDs.
OO Use the key to toggle between the
following illuminator segments: full ring,
Switching on the focus column half ring (left–right), half ring (front–rear),
1. Switch on the focus column using the quarter ring, opposite quarter rings, eighth
power switch on the rear in order to supply ring.
the Leica LED5000 RL with current.
OO Use the and keys to rotate the active
OO Use the key to switch the illuminator on segments clockwise or counterclockwise.
or off.
OO Use the or keys to adjust the bright-

ness in 10 increments.
OO Tap the or key briefly to adjust the

intensity in small increments. Hold one
of the keys to change the intensity more
quickly.

Leica LED5000 RL: Use (Continued)
OO If you press or for approx. 2 seconds,

the segments rotate automatically until
you press one of these keys again. If you
change the segment using the key, the
automatic changeover is kept. Pressing
or briefly stops the changeover.
OO Press and hold the key for approx. 2

seconds to switch on the full ring of the ring
illuminator.
The active illuminator segments are

indicated on the front control panel by
8 LEDs arranged around the on/off key.

Leica LED5000 NVI: Assembly on High-Performance Stereo Microscopes
(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A)
Assembly of the Leica LED5000 NVI 2. Place the Leica LED5000 NVI on the focus- 3 Press the Leica LED5000 NVI backwards to
(10 450 659) ing column so that the screw fits into the the focusing column and screw it in place
1. The Leica LED5000 NVI in factory configu- thread provided and the lug fits into the using an Allen key M4.
ration is already assembled with the exten- groove.
sion plate (for assembly on manual and
motorized focusing columns) at the factory.

(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) (Continued)
4. Unscrew the three Phillips screws on the 5. Set the optics carrier on the adapter of the 6. Install the remaining microscope compo-
objective mount of the optics carrier and Leica LED5000 NVI and tighten it with an nents (tube, ergonomic accessories, camera
remove the spacer ring. Allen key M4. etc.) according to the corresponding user
manuals.
7. If the focusing column is equipped with

integrated electronics, connect the Leica
LED5000 NVI to the focusing column via
the CTL2 connection. Alternative instal-
lation for manual columns: Connect the
external power supply unit (10 450 266) to
the Leica LED5000 NVI.

(Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A) (Continued)
8. Screw the objective to the Leica Lists of recommended objectives that can be
LED5000 NVI from below. used with the Leica LED5000 NVI without addi-
tional adapters:
10 411 597 Achromatic objective f=100 mm

10 450 027 Planapochromatic objective 0.63×
Additional objectives can be used by means of

adapters. However, loss of optical quality does
need to be expected (e.g. vignetting, color
seams, reflections etc.).

Leica LED5000 NVI: Installing Optional Accessories
Polarization set 2. The analyzer is installed between the tube

The Leica LED5000 NVI can be equipped with and optics carrier. To do so, remove the tube
an optional polarization set. Using the polariza- from the optics carrier. Place the analyzer
tion set, reflections can be reduced on metallic on the optics carrier and then install the
surfaces, for example. tube. Fasten the components by tightening
the positioning screws.
1. The polarizer is inserted into the filter insert
on the bottom of the Leica LED5000 NVI.
3. To generate the desired polarization effect,

rotate the rotary disk of the analyzer until
the desired image quality is reached.

Leica LED5000 NVI: Installing Optional Accessories (Continued)
Filter inserts 1. To disassemble the filter holder, push the

The Leica LED5000 NVI is supplied with a filter holder approx. 1 cm to the side and
filter holder as a standard feature. Commer- remove the holder downwards out of the
cially available filters of size 1/2“ can be used. Leica LED5000 NVI. The filter holder is
Make sure that the filters being used are suffi- assembled in the reverse order of steps.
ciently temperature-resistant, so they are not
damaged by the high-energy illumination.
Additional empty filter inserts (10 725 035) can
be purchased as spare parts.

Quick Start Guide
Leica M Series User Manual Quick Start Guide49

The Fastest Route to Success
Your Leica stereo microscope has been delivered in completely assembled

condition by your Leica partner, and naturally you want to get right to
work. Therefore, your next step should be to study the Quick Start Guide,
which outlines the most important steps at a glance.
This manual will then familiarize you with the finer details of your micro-
scope. The following pages contain important, practical information that
makes using it every day easier.
Take time to read it – it's worth it!
The M series quick start guide (included with instrument)

Overview of an M Series Microscope
1. Magnification changer (zoom)

2. Switch click stops on/off
3. Coarse/fine focusing
4. Fastening screw for the binocular tube
(or accessories)
7
5. Interchangeable objective
8 9
6. Adjustable eyepiece tubes 6
7. Eyepieces for spectacle wearers with dioptric 4
2
correction and eyecups 11 1
8. Fastening screws for the eyepieces
9. Trinocular tube 10
3
10. Built-in iris diaphragm

The Correct Interpupillary Distance
The interpupillary distance is correctly set if you Adjusting the interpupillary distance 3. Slowly approach the eyepieces with your
see a single circular image field when looking 1. Look into the eyepieces. eyes until you can see the complete image
at a specimen. field without corner cutting.
2. Hold the eyepieces with both hands. Push
If you are still a novice microscope user, you the eyepieces together or separate them
may need a short time to become accustomed until you see a circular image.
to this. Not to worry – after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece
measures approx. 22 mm for 10/23B wide-field
eyepieces for persons wearing glasses.
 

Using the Eyepieces
The eyepieces form the connection between If you do not wear glasses: If you wear glasses:
the tube and the eye of the observer. Simply 1. Hold the eyepiece firmly and rotate the 1. Hold the eyepiece firmly and rotate the
push them into the tube and they are ready to eyecups forwards counterclockwise. eyecups counterclockwise towards the rear,
use. as otherwise the viewing distance is too
2. If an eyepiece is equipped with the inte- great.
Each eyepiece offers a certain magnification grated dioptric correction, turn the value to
factor that has a determinative effect on the the "0" mark.
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical grati-
cules that enable measuring and quantifying of
specimens.
Dioptric correction
A built-in dioptric correction is available for 2. If an eyepiece is equipped with the inte-
eyeglass wearers. For more information, refer grated dioptric correction, turn the value to
to page 63. the "0" mark.
By the way, one benefit of viewing with

eyeglasses is a drastically lower risk of bacte-
rial transmission (see page 62). The soft mate-
rial of the eyecup also ensures that your glasses
will not be scratched, even if they contact the
eyepiece.

Focusing
Focusing raises or lowers the stereo microscope Focusing Coarse/fine adjustment

using the focusing drive. The specimen detail is OO The inner, coarse adjustment is used for The sharpness is adjusted using the coarse/fine
brought into sharp focus as soon as it is in the covering great distances. adjustment.
focal point of the objective.
OO The outer, fine adjustment is used for fine OO The resolution of the coarse/fine adjust-
OO The focusing drive can be operated either focusing. ment is 1 µm.
left- or right-handed.
• load of up to 15 kg.
The coarse/fine adjustment carries a

Adjusting the Resistance of the Focus Drive
Adjusting the resistance

Is the focus movement too loose or too tight?
Does the outfit tend to slide downwards? The
resistance can be adjusted individually depend-
ing on the equipment weight and personal
preferences as follows:
1. Grip the outer drive knobs with both hands

and turn them towards each other until
the desired resistance is reached during
focusing.

Changing the Magnification (Zoom)
All M series microscopes have an integrated Zooming

zoom. The name indicates the zoom range 1. Look into the eyepieces.
covered:
2. Focus on the specimen.
OO Leica M125 C = 12.5:1
OO Leica M165 C = 16.5:1 3. Rotate the magnification changer until the
OO Leica M205 C = 20.5:1 desired magnification is configured.
� Leica M205 A = 20.5:1
The rotary knob for the zoom can be used either

left or right-handed.

Ratchet Steps and Magnification Levels
The zoom button can optionally be operated Enabling and disabling ratchet steps Magnifications and fields of view
either with or without ratchet steps. Continu- 1. Push the top button downwards to disable The formula on page 104 provide additional
ous zoom is possible when the ratchet steps are the ratchet steps. information about the magnifications and field
disabled, which many users find convenient. of view diameters, with consideration given to
On the other hand, when the ratchet steps are 2. Push the bottom button upwards to enable the position of the magnification changer and
enabled, photographs, measurement results the ratchet steps. the eyepiece and objective combination used.
etc. can be reproduced more accurately.

Parfocality: More Comfort and Convenience for Your Work
All Leica stereo microscopes are parfocally Parfocality

matched, meaning that you can view a focused 1. Enlarge the view to the maximum level.
specimen from the lowest to the highest
magnification without having to refocus. There 2. Focus on the specimen.
is no need to readjust the focus to view of speci-
men location that is located higher or lower. You are done! Even if you select a smaller work-
ing distance, the specimen remains pin-sharp.
Requirements for parfocal work
OO If you are using an eyepiece with dioptric The parfocality is maintained until you focus on
correction, the procedure differs from this another level of the specimen.
description. For more information, refer to
page 64.
OO For the procedure for adjusting the parfo-

cality for the objective nosepiece, refer to
page 26.

Iris Diaphragm
The iris diaphragm in the optics carrier of your Closing the iris diaphragm Opening the iris diaphragm
M series microscope has the same purpose as OO Close the iris diaphragm by turning the OO Open the iris diaphragm by turning the
those in a camera shutter: it regulates the avail- knob to the left. The subject appears darker knob to the right. The subject now appears
able light, which changes the depth of field. The and the depth of field increases. brighter, but the depth of field decreases.
"depth of field" is the area of a specimen that is
brought into sharp focus.

Eyepieces
Leica M Series User Manual Eyepieces60

Magnification Factors of the Eyepieces
An eyepiece not only makes it possible to look passively into the micro-
scope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10× and 40×.
The following eyepieces are available for the M series:

Magnification Dioptric correction Order number
10× ± 5 diopter settings 10 450 630

Health Notes
Potential sources of infection
•
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye. The risk
can be kept to a minimum by using individual
eyepieces or detachable eyecups. Eyecups can
be ordered separately. Please contact your
Leica partner.
Separate eyecups are an effective way of preventing infections.

Dioptric Correction
All Leica eyepieces are also available with built- Using the dioptric correction Note that when using dioptric correction,
in dioptric correction, allowing the microscope 1. Set the dioptric correction of both the advantage of parfocality is lost – thus
to be used without glasses even by those with eyepieces to the mid position ("0" diopter you have to manually refocus each time you
vision problems. The correction comprises ±5 settings. change the zoom level. To also use parfocality
diopter settings. with dioptric correction, refer to the instruc-
2. While wearing your glasses, look through tions on page 64.
the eyepieces and focus on the specimen.
3. Rotate both eyepieces to the maximum

value of "+5".
4. Hold one eye closed and rotate the other

eyepiece in "–" direction until the specimen
appears sharp.
5. Then, open the other eye and correct the

diopter settings until the image is uniformly
sharp.

Dioptric Correction and Parfocality
Leica stereo microscopes are parfocally Adjusting 7. Look into the eyepieces.
matched. The prerequisite for this is the correct 1. Set the dioptric correction for both
setting of the diopters and the parfocality. The eyepieces to "0". 8. Slowly rotate each eyepiece individually
following adjustments only have to be carried in the "–" direction until each eye sees the
out once by each user. 2. Select the lowest magnification and focus object sharply imaged.
on a flat specimen.
9. Select the highest magnification and refo-
Preparations 3. Select the highest magnification and read- cus if necessary.
OO Move the lever of the video/phototube to just the sharpness.
the "observation" position and open the Now, if you adjust the magnification from the
diaphragm. 4. Select the lowest magnification again, but lowest to the highest level, the specimen is
do not look into the eyepieces. always brought into sharp focus. If not, repeat
OO If you are using the microscope carrier AX, the process.
set it to stereoscopic observation. 6. Rotate the eyepieces counterclockwise in
the "+" direction as far as they will go (+5
diopter settings).

Graticules
Use 2. Clamp the graticule on the insert, applying 4. You can now align the graticule by rotating
Graticules make length measurements and moderate pressure. Ensure that the grati- the eyepiece in the tube and then tighten-
counting easier, particularly for workstations cule fits tightly. ing it using the clamping screw.
that are not equipped with a digital camera and
LAS X software.
The graticules for length measurements

and numbering are fitted in mounts and are
inserted into the eyepieces.
1. Screw the insert off of the eyepiece.

3. Screw the insert and graticule firmly into
place and replace the eyepiece in the tube.
Use with the AX carrier
If possible, measure with the micro-
scope carrier AX in vertical position. The
measurements are more accurate without the
convergence angle in the stereoscopic image.

Photography & Video
Leica M Series User Manual Photography & Video66

Photography & Video
For most microscope users, digital documen- Leica cameras Leica Application Suite X
tation has become an invaluable part of their However, if you require absolute control The "Leica Application Suite X", or "LAS X" for
work. Research results can be presented in an over the camera and need the capability for short, is, as it were, the digital extension of the
attractive manner; measurements on the digi- measurement, evaluation and more in addition Leica M series microscopes. In addition to
tal image provide clarity and, in conjunction to photography, the digital Leica cameras are capturing images, it lets you control the micro-
with scanning stages, even images of large exactly right for you. Together with the Leica scope, illuminator, stages, cameras and more.
specimens can be captured step by step and Application Suite X, they provide virtually limit- For additional information, refer to the LAS X
automatically joined to create a new complete less freedom of use. For additional informa- online help.
image. tion about Leica cameras, refer to the camera's
documentation.
Adapter
If camera control using the Leica Application
Suite X is not required, conventional mirror
reflex and rangefinder cameras from third-
party manufacturers can be used. For this
purpose, Leica Microsystems offers a variety
of adapters that can be used together with the
50 % and 100 % trinocular tubes.

Photo Tubes and C-mounts
Application Alternatively, you can also use a C-mount To do so, instead of the C-mount adapter,
All Leica DFC/DMC/MC cameras are equipped adapter with higher magnification. This simply use the corresponding SLR adapter
with a standardized C-mount interface. In primarily avoids the critical border area of the with T2 connection. However, these third-
turn, the C-mount adapter for the respective field of vision and concentrates on the center of party cameras are not integrated into the Leica
trinocular tube is connected to this interface. the field of vision. Application Suite X and have to be operated
This adapter creates a solid mechanical connec- using the corresponding software from the
tion between the microscope and camera and Cameras from third-party suppliers camera manufacturer.
ensures optimum rendering of the microscopic In addition to Leica cameras with the
image on the image sensor of the camera. standardized C-mount interfaces, you can The Leica digital cameras are detailed in a
connect third-party cameras to the microscope separate user manual along with instructions
Usually, the ideal is for the digital camera using a T2 bayonet adapter. for their assembly and use.
to capture as much of the field of vision as
possible, while excluding as much of the black
Photo tubes and C-mounts
edge of the field of vision as possible. To do
10 445 928 Video adapter 0.32× C-mount interface for cameras with 1/3" sensors
so, the magnification factor of the C-mount
10 450 528 Video adapter 0.5× C-mount interface for cameras with 1/2" sensors
adapter must match the image format of the 10 447 367 Video adapter 0.63× C-mount interface for cameras with 2/3" sensors
sensor as closely as possible (see table). 10 446 307 Video adapter 0.8× C-mount interface for cameras with 2/3" sensors
10 450 829 Video adapter 1.0× C-mount interface for cameras with 1" sensors
If there is unwanted shading at the corners even 10 450 317 Video adapter 1.0× HC C-mount adapter for Leica HC C-mounts
with a compatible C-mount adapter installed, it 10 445 930 Video adapter 1.0× delta For Delta C-mount adapter with diameter 37 mm
can be corrected using the "Shading function" 10 447 436 DSLR adaption 1.6× For APS-C format cameras
of the camera software. 10 446 175 DSLR adaption 2.5× Use in combination with 10 445 930 for 35 mm format cameras

Trinocular Video/Phototube 50 %
Use
With its third beam path, the trinocular video/
phototube 50 % enables you to simultaneously
view and photograph a specimen. The available
light is divided as follows:
OO 50 % is available for the two eyepieces.
OO 50 % of the light is diverted to the video/

photo beam path.
Assembly
Fasten the "trinocular tube 50 %" to the optics
carrier instead of the binocular observation
tube (refer to page 22).

Trinocular Video/Phototube 100 %
Use Switchover
With its third beam path, the trinocular video/ OO Turn the controller on the right side of the
phototube 100 % enables you to either view or tube into the horizontal position in order to
photograph a specimen. This means that 100 % guide all available light into the eyepieces.
of the light is available to one or the other beam You can now observe the specimen.
path. The other beam path remains opaque or
black. OO Turn the controller on the right side of the
tube into the vertical position in order to
Assembly guide all available light into the camera.
Fasten the "trinocular tube 100 %" to the optics You can now photograph the specimen.
carrier instead of the binocular observation
tube (refer to page 22).

Trinocular Video/Phototube 100 %: ErgoTube 5°– 45°
Use
The trinocular ErgoTube can provide the user a
comfortable and ergonomic operating position
by allowing the user to fine-tune the viewing
angle of the eyepieces to best fit their posture.
The photo slider switches the right beam path

from 100 % of light to the eyepieces to 100 %
light to the camera port. In photo position the
left beam path is closed to prevent stray light
entering the tube.
Assembly
Before mounting the ErgoTube, please remove
the transport lock screw under the tube. Other-
wise the photo slider cannot be used.

Microscope Carrier
Leica M Series User Manual Microscope Carrier72

The Microscope Carrier AX
Users who intend to do a great deal of photog- Stereoscopic viewing

raphy, take measurements or work with polar- A stereoscopic view is required for examining
ization should select the microscope carrier AX spatial objects. For this purpose, the micro-
for stereoscopic/axial observation. The paral- scope carrier AX must be brought to its home
lax-free imaging by means of a perpendicular position.
beam path leads to results without parallax
error. 1. Unscrew the clamping screw.
Compatibility
The planachromatic and planapochromatic
objectives fit into the thread in the microscope
carrier AX for the Leica M125 C, Leica M165 C,
Leica M205 C and Leica M205 A.
2. Push the microscope into the home posi-

tion (mid position) to obtain a spatial view
and tighten the clamping screw.

The Microscope Carrier AX (Continued)
Parallax-free images The objective is now located directly beneath

The parallax-free imaging produces more either the left or the right beam path.
precise results in photography, measuring and
polarization. Coaxial light and the Leica M205 C /
Leica M205 A
1. Unscrew the clamping screw. When using coaxial incident light together with
the Leica M205 C / Leica M205 A, unscrew the
2. Push the microscope towards the left as clamping screw and press the optics carrier
far as it will go and tighten the clamping towards the right (caution: the movement is
screw. only 2 mm). This provides uniform light inten-
sity in both beam paths.

The Objective Nosepiece
Use Parfocal work Technical constraints
•
The objective nosepiece enables you to switch With the new M series objectives, the parfo- The objective nosepiece cannot be
between two objectives using just one hand cality is maintained even when objectives are used together with the LED5000 MCI
movement, for example between a 1× and a 2× changed, meaning that the specimen remains system illumination, as the objective nosepiece
planapochromat. in sharp focus during the change. can no longer be rotated.
Older Leica objectives can continue to be

used, but without parfocality during the
objective change.

Objectives
and Optical Accessories
Leica M Series User Manual Objectives and Optical Accessories76

The Different Types of Objectives
To meet the various requirements regarding Achromatic objectives Achromatic objectives with a long focal
imaging properties, there is a choice of high- The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2× length:
quality interchangeable planachromatic and achromatic objectives offer countless variants For special applications achromatic objectives
planapochromatic objectives and also lower- for selecting the object field diameter, magni- with long working distances and focal lengths
priced interchangeable achromatic objectives. fication ranges and working distances (see of f=100 mm to 400 mm are available.
page 104).
OO Achromatic objectives are particularly
suited for specimens with high-contrast Planachromatic objective 1×
structures. For the highest requirements for overall image
quality, we recommend equipping the micro-
OO Flat-field (planachromatic) objectives are scope with the 1× plan (flat-field) objective,
particularly well suited for studying flat which returns sharp, contrast-rich object fields.
objects such as wafers and thin sections.
OO With planapochromatic objectives, the

finest structures are visible with high
contrast. The sophisticated apochromatic
correction allows these objectives to attain
the highest color brilliance and fidelity.
Leica M Series User Manual Objectives and Optical Accessories77

Bases
Leica M Series User Manual Bases78

Transmitted-light Base Leica TL BFDF: Controls
Button to toggle between bright field/dark field Extension plate of the transmitted-light base TL BFDF Connector for cold light sources
(light conductor active f = 10 mm,
end tube f = 13 mm)

Transmitted-light Base Leica TL BFDF: Operation
Light intensity control Bright field Dark field
•
Please observe the user manual – in Bright field is suitable for examining translu- In dark-field illumination, a ring illuminator is
particular, all safety regulations – from cent objects featuring contrasting structures. used in such a way that the direct light does
the manufacturers of the light guide and cold The object is directly illuminated from below not reach the objective without a specimen.
light source. and is seen in its natural colors against a bright Only the structure of semitransparent, opaque
background. objects disperses the light, making the object
OO Switch on the cold light source according to visible against a dark background.
the manufacturer's user manual and adjust OO Turn the control as far as it will go towards
the brightness. "BF" ("bright field"). OO Turn the control as far as it will go towards
"DF" ("dark field").
Transmitted-light control
The Leica TL BFDF transmitted-light base has
a control that switches the light from "bright
field" to "dark field".
Fingertip with bright field illumination Identical subject with dark field illumination

Leica IsoPro (Non-motorized): Controls
Operating the XY stage IsoPro

1. To move the stage in X direction, rotate the
outer knob.
2. To move the stage in Y direction, rotate the

inner control ring.
1 2

System Illumination
Leica M Series User Manual System Illumination82

Leica LED5000 MCI
Use Contact with the base
•
The Leica LED5000 MCI (for "Multi Contrast If the optics carriers are accidentally
Illumination") is a universal high-output illu- lowered too far, contact between the
minator. Three groups of 3 LEDs each can be base and MCI may result. A safety mechanism
arranged as desired around the specimen. The in the linkage ensures that, in this case, the MCI
targeted control of the LEDs enables various is automatically pushed upwards to prevent the
illumination scenarios to be implemented. possibility of damage.
Preparation Installed too high OO Be sure not to place any specimens directly
Hold the MCI with both hands and pull it down- beneath the MCI.
wards until the illuminator clicks into place on
the bottom end of the guide rods. OO After the optics carrier is lifted, put the MCI
back into the original position.
In this position, you always have the same
contrast with identical illumination. This guar-
antees the reproducibility of an experiment.
Optimum height

Leica LED5000 MCI (Continued)
Using the keyboard The illumination scenarios

OO Use the key to switch the illumination on Use the " " and " " keys to toggle between the
or off. illumination scenarios shown below.
OO Use the "+" and "–" keys to adjust the Maximum brightness
brightness in 10 increments. Touch either For temperature reasons it is not possible to
of the two buttons to adjust the intensity in switch on all nine LEDs simultaneously. There-
small increments. Hold a key to change the fore, to provide bright overall illumination, the
intensity more quickly. top two rows are activated. The bottom row
primarily provides contrast.
The Leica LED5000 MCI can also be controlled by
the LAS X (Leica Application Suite X) software.
Maximum brightness Maximum contrast Point illumination Point illumination Point illumination
from the rear from the left from the right

Leica LED5000 RL
Use Using the keyboard The illumination scenarios

The Leica LED5000 RL ("Ring Light") generates OO Use the key to switch the illumination on Use the " " and " " keys to toggle between the
a very bright and homogenous incident light. or off. illumination scenarios shown below.
It has a diameter of 80 mm and illuminates the
specimen with 48 LEDs that can be switched on OO Use the "+" and "–" keys to adjust the Constraint
and off completely or in various combinations. brightness in 10 increments. Touch either The Leica LED5000 RL has been designed for
It is controlled using either the integrated or via of the two buttons to adjust the intensity in use with the 1.0× and 0.63× objectives. For the
the Leica Application Suite X (LAS X). small increments. Hold a key to change the 1.6× and 2.0× objectives, conventional ring
intensity more quickly. illuminators from the Leica illuminator product
LAS X enables you to create fully reproducible range are available.
illumination scenarios and automatically toggle
between them. For additional information,
refer to the LAS X online help.

Accessories
Leica M Series User Manual Accessories86

Leica Hand Wheel and Foot Switch
Up to five foot switches can be configured with

a microscope system. They can be programmed
in LAS X to control focus, illumination, and indi-
vidual memory positions.
The hand wheel is designed to control the

motor focus.

Leica SmartTouch
With the touchscreen of the Leica SmartTouch, For a detailed description of the functions and
you can control your experiment procedures settings, please refer to the Leica SmartTouch
with a few hand movements and never lose manual or the Leica Application Suite X help
sight of all your important optical parameters. file.
The most important control functions on the

control unit can be adjusted to your specific
needs with freely programmable rotary knobs
and function buttons.
Controls
The functions of the individual knobs and
screen elements can be configured both
directly on the Leica SmartTouch and using the
Leica Application Suite X.
The standard configuration when shipped from

the factory is as follows:
OO Top part of the knob = zoom

OO Upper part of the knob = focus

Dimensional Drawings
Leica M Series User Manual Dimensional Drawings89

292
217
Leica M125 C / Leica M165 C
455
Leica M125 C / Leica M165 C with incident light base and new focusing column
24
(dimensions in mm) 120 120
292
217
400
455
24
120 120
277

400
455
24
120 120
277

496.5
Leica M125 C / Leica M165 C (Continued)
431
522
496.5
Leica M125 C / Leica M165 C with transmitted-light base TL3000 Ergo and manual XY stage
(dimensions in mm) 154 187
91
118
431
522
Y = +-50 mm
154 187
300
496.5
91
118
370
Y = +-75 mm
431
Y = +-50 mm

300
154 187
91
118
370
Y = +-75 mm
-50 mm

300
45
132
22
36
112
45
Leica LED5000 MCI
157
112
90
Leica LED5000 MCI
(dimensions in mm)
45
132
45
132
22
22
83.5
36
112
36
112
275.3
45
45
157
157
112
112
90
90

83.5
83.5
275.3
275.3

Leica LED5000 RL
Leica LED5000 RL
(dimensions in mm)
37
37
132 160
160
58 mm
132

Leica LED5000 NVI
Leica LED5000 NVI for high-performance stereo microscopes

(dimensions in mm)

Leica MATS TPX
170
134
98
140 11
190
220 4
10 447 275
10 447 164

Specifications
Leica M Series User Manual Specifications96

Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A
Leica M125 C Leica M165 C Leica M205 C / Leica M205 A
Optical data
Zoom 12.5 : 1 manual 16.5 : 1 manual 20.5 : 1 manual/motorized

with FusionOptics
Data with standard optics

(1×objective/10× eyepieces)
– Zoom range 8×–100× 7.3×–120× 7.8×–160×
– Resolution max. 432 lp/mm max. 453 lp/mm max. 525 lp/mm
– Working distance 61.5 mm (planapochromatic) 61.5 mm (planapochromatic) 61.5 mm (planapochromatic)
– Object field ∅ 28.8 mm – 2.3 mm ∅ 31.5 mm – 1.92 mm ∅ 29.5 mm –1.44 mm
Maximum values (based on optics

combination)
– Magnification 800× 960× 1,280×
– Resolution 864 lp/mm 906 lp/mm 1,050 lp/mm
– Visible structural width 579 nm 551 nm 476 nm
– Numerical aperture 0.288 0.302 0.35
– Object field ∅ 68 mm ∅ 63 mm ∅ 59 mm
Working distances 135 mm (0.5× planachromatic)

112 mm (0.8× planachromatic)
67 mm (0.63× planapochromatic)
61.5 mm (1× planapochromatic)
30.5 mm (1.6× planapochromatic)
20.1 mm (2× planapochromatic)

Leica M125 C / Leica M165 C / Leica M205 C / Leica M205 A (Continued)
Optics carrier
100 % apochromatic optical system CMO (Common Main Objective) lead-free
Specific surface resistivity 2×1011 Ω/mm2

(housing) discharge time <2 seconds from 1,000 V to 100 V
Coded/motorized function Zoom, iris diaphragm, Zoom, iris diaphragm, Zoom, iris diaphragm,
objective nosepiece objective nosepiece objective nosepiece
(coded only) (coded only) (encoded and motorized)
Engageable zoom notches 12 for repetitive tasks 13 for repetitive tasks 14 for repetitive tasks (M205 C)
Continuously variable control with
SmartTouch or LAS X (M205 A)
Double-iris diaphragm for Built-in and encoded Built-in and encoded Installed and encoded (M205 C) /
depth of field control motorized (M205 A)

Accessories
Standard objective (parfocal

1× planapochromatic
in the objective nosepiece)
Additional objectives (parfocal in the

2× planapochromatic, 1.6× planapochromatic, 0.63× planapochromatic
objective nosepiece)
Objective revolver, parfocal Fast magnification change Fast magnification change Fast magnification change
0.63×–2× planapochromatic, coded 5.12× – 200×, parfocal 4.67× – 240×, parfocal 4.99× – 320×, parfocal
Binocular tubes, ergonomics – Apochr. binocular and trinocular tubes with synchronized interpupillary distance adjustment
– Various ErgoModules (optional)
Interpupillary distance 50 mm – 105 mm with the new trinocular ErgoTube®
Wide-field eyepieces for

10×, 16×, 25×, 40×, with replaceable eyecups & click stop
eyeglass wearers
Manual coarse/fine focus Focusing range 130 mm / 130 mm, adjustable ease of movement
Microscope carrier AX for

For convergence-free imaging without lateral shift of the specimen
stereo or vertical observation
PC interface USB

Modular System Stands, illuminators
Transmitted-light base TL4000 BFDF: Bright and circular dark field

TL3000 Ergo: Bright field, dark field, Rottermann Contrast
TL5000 Ergo: Bright field, two-sided dark field, Rottermann Contrast
Incident-light base Large incident-light base with black-and-white stage insert and Antishock feet
Stages Gliding stage, MATS heating stage, cup stage, rotatable polarization stage, Leica IsoPro XY stage
(manual)
Illuminators LED5000 RL, LED5000 SLI, LED5000 MCI, LED5000 HDI, LED5000 NVI, LED5000 CXI, cold light sources
Misc. accessories
Photography, video Digital image capture systems from Leica, various camera variants (e.g. integrated and external),
various adapters for commercially available analog and digital cameras
Image archiving, image processing Leica Application Suite X (LAS X) software, consisting of basic program and various auxiliary modules
Measurement reticules For length measurements and counting
Vertical and oblique observation 45° side view around the complete object
Drawing tube for left and right-handed users
Discussion tube for training

for two observers
for two observers

Transmitted-light Base Leica TL BFDF
Light source External via cold light source

Illuminated area 40 mm
Connections Connection for cold light guide,
active f=10 mm, end tube f=13 mm
Weight 5.8 kg
Illumination types
Bright field Yes
Dark field Yes
Oblique light No
Relief Contrast System (RC) No
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control Yes*
Integrated filter holder No
Coated optics for increasing the color No
temperature
Matching of high num. aperture Yes**
Remote control options Yes***
AntiShock Pads Yes
Dimensions (W×H×D) 340×390×90 mm
*With cold light source Leica CLS150 LS **Plan mirror ***With external light source

Motorized Focus Drives With "Zoom" Carrier
(M125 C, M165 C, M205 C, M205 A)
Technical data
Environment
Operation temperature 10 °C – 40 °C
Storage temperature -10 °C – 55 °C
Humidity (operating / storage) 10 % – 90 % RH (non condensing)
Operating altitude (maximum) 0 – 2,000 m
Storage altitude nA
Pollution degree of intended environment 2 (office / lab environment)
Accessories Various Leica controlling devices and
Leica illumination devices
Ports and connectors 1 USB (2.0)
3 CAN
1 power (33 V)
Power supply
Input 100 – 230 VAC ± 10 %, 50/60 Hz, 0.5 A – 1.2 A
Output 33 VDC, max 3.03 A (100 W)
Overvoltage category II

Appendix
Leica M Series User Manual Appendix103

Calculating the Total Magnification and Field of View Diameter
Parameter Example
MO Magnification of objective MO 1× objective
ME Magnification of eyepiece ME 25×/9.5 eyepiece
z Magnification changer position z Zoom position 4
q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° q Coaxial reflected light 1.5×, tube factor
ErgoTube r Factor 1.25×
r Factor 1.25× if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Calculation example: magnification in binocular tube Calculation example: field of view diameter in the specimen
MTOT VIS = MO × ME × z × q × r NFOV

or ∅ OF:
MO × z ×q ×r
1 × 25 × 4 × 1.5 × 1.25 = 187.5×

Care, Maintenance, Contact Persons
We hope you enjoy using your high-perfor- Contact address Care

mance microscope. Leica microscopes are However, if your instrument should no longer OO Protect your microscope from moisture,
renowned for their robustness and long service function properly, contact your technician, fumes and acids and from alkaline, caustic
life. Observing the following care and clean- your Leica representative or Leica Microsystems and corrosive materials and keep chemicals
ing tips will ensure that even after years and (Switzerland) AG, CH-9435 Heerbrugg. away from the instruments.
decades, your Leica microscope will continue to
work as well as on the very first day. OO Plugs, optical systems and mechanical parts
E-mail contact: must not be disassembled or replaced,
Warranty benefits stereo.service@leica-microsystems.com unless doing so is specifically permitted
The guarantee covers all faults in materials and described in this manual.
and manufacture. It does not, however, cover
damage resulting from careless or improper OO Protect your microscope from oil and
handling. grease.
OO Do not grease guide surfaces or mechani-

cal parts.

Care, Maintenance, Contact Persons (Continued)
Protection from dirt Cleaning polymer components Permitted measures

Dust and dirt will affect the quality of your Some components are made of polymer or are OO Clean the microscope (or parts of it)
results. polymer-coated. They are, therefore, pleasant using warm soapy water, then wipe using
and convenient to handle. The use of unsuit- distilled water.
OO Put a dust cover over the instrument when able cleaning agents and techniques can
it will not be used for a long time. damage polymers, OO For stubborn dirt, you can also use ethanol
(industrial alcohol) or isopropanol. When
OO Use dust caps to protect tube openings, doing so, follow the corresponding safety
tubes without eyepieces, and eyepieces. instructions.
OO Keep accessories in a dust-free place when OO Remove dust with a pneumatic rubber bulb
not in use. or with a soft brush.
OO Clean objectives and eyepieces with special

optic cleaning cloths and with pure alcohol.

Subject to modifications. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.
11/2018 · 10IDM19060EN_1 · © 2018 by Leica Microsystems GmbH.
CONNECT
WITH US!
Leica Microsystems (Schweiz) AG · Max-Schmidheiny-Strasse 201 · 9435 Heerbrugg, Switzerland

T +41 71 726 34 34 · F +41 71 726 34 44
www.leica-microsystems.com

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Safety concept Use in clean rooms Servicing

OO In most cases, we can provide special solu-

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The "Safety concept" booklet contains addi-

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Repairs, service work Integration in third-party products Health risks

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Technical safety equipment – Safety – Part one: general

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Handling electrical components

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Always position the microscope so that

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General Instructions 2 Leica IsoPro Manual XY Stage: Assembly 31

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Eyepieces System Illumination

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Ever since their introduction by Horatio S. Limits are made to be broken.

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2. Insert the four screws provided into the

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