Microscope Manual Leica Z16 APO
Microscope Manual Leica Z16 APO
Microscope Manual Leica Z16 APO
•
This symbol indicates especially impor- This symbol warns against touching
tant information that must be read and accessible hot surfaces, e.g. those of
complied with. light bulbs.
This symbol indicates additional infor-
★★ Functional disturbances or damaged inst- mation or explanations that intend to
ruments provide clarity.
This symbol indicates especially impor- ★★ This symbol within the text stands for addi-
tant information that is mandatory to tional information and explanations.
read and observe.
infections of the eye. The risk can be kept to a Warning Supply unit
minimum by using personal eyepieces for each UV radiation could damage the eyes. Therefore: Always unplug the supply unit from the power
individual or detachable eyecups. ★★ Never look into the light spot on the speci- supply:
men plane without a UV protection screen. ★★ When installing and disassembling the
lamp housing
Light sources: Safety Instructions ★★ Never look into the eyepieces if no excita-
Protective measures of the manufacturer: tion filter is in the beam path. ★★ Before opening the lamp housing
★★ UV protection screen in front of the speci-
men plane prevents the user from looking ★★ Fill empty filter positions with dummy filter ★★ When replacing the high-pressure mercury
directly into the UV rays. carriers. lamp and other parts, such as the heat-
absorbing filter or the collector
★★ Dummy filter carriers in the free positions ★★ Do not select a white, strongly reflective
of the rapid filter changer prevent direct UV background for the specimen. ★★ During maintenance work on the supply unit
radiation from reaching the eyes.
Appendix
Calculating the Total Magnification and Field of View Diameter 116
Care, Maintenance, Contact Persons 117
Congratulations on obtaining your new Leica Z series macroscope. We are Though the reliability and robustness of Leica macroscopes is legendary,
convinced it will exceed your expectations, as the Z series embodies all like any high-tech product, the Leica Z series requires a certain degree of
the qualities you associate with the name Leica Microsystems: excellent care and attention. Therefore, we recommend that you read this manual.
objectives, high-quality engineering, and reliability. Furthermore, the It contains all the information you need regarding operation, safety and
modular design ensures that the Z series adapts perfectly to your needs— maintenance. Simply observing a few guidelines will ensure that even
no matter which accessories you require for your tasks. after years of intensive use, your Leica macroscope will continue to work
as smoothly and reliably as on the very first day.
Thanks to the parallax error free observation, measurements are more
accurate and adaptation tasks are easier. Together with a Leica digital We wish you the best of success in your work— after all, you are now
camera, you obtain the maximum amount of high-quality data, which equipped with the best tool!
also makes later evaluations on the computer much easier.
The Leica Z series provides maximum flexibility Despite this, you will notice that the controls tation when it comes to devising customer-
in choosing equipment, thanks primarily to the and individual components do not differ signi- specific solutions. If you have a special request
modular configuration and the compatibility ficantly. Whichever configuration you choose, that cannot be met with standard parts, contact
that Leica has painstakingly maintained for you will quickly feel right at home with your your Leica consultant. He or she has a solution
decades. The optics carriers, eyepieces, bases new macroscope. for every problem.
and more can be combined in any way you
choose, allowing you to create the macroscope
that best suits your needs. Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
Reliability for your experiments Control à la carte tically* or turns a series of individual images
For the Leica Z6 APO A and Z16 APO A auto- You alone determine the degree of automation. into one large, seamless illustration**, to give
mated macroscopes, motorized control of the The zoom, focus and illumination can be opti- only two examples.
zoom, iris diaphragm and fine focus is possible. onally controlled using the "Leica Application
This guarantees not only convenient work, but Suite" (LAS) software solution or via the Leica Talk to your Leica partner about the wide range
also added reliability for your experiments, as SmartTouch touchscreen panel. of automation possibilities – he or she will show
results can be reproduced exactly. In conjunc- To automate your workflows even more, also you how to get the maximum out of your Leica
tion with the Leica LED3000 NVI™ system illumi- integrate the Leica IsoPro™ motorized XY stage macroscope.
nators, a wide variety of scenarios can be set up, – it positions multiple specimens fully automa-
saved and restored at the touch of a button.
The Leica SmartTouch controls all motorized The Leica Application Suite (LAS) controls and
functions of the Z series and the connected photographs fully automatically as desired.
accessories.
If your new Leica macroscope has already been If, on the other hand, you are assembling the
assembled and commissioned by your Leica Leica macroscope yourself, continue with the
consultant, click here to skip through the instal- "Assembly" chapter, which begins on Page 17.
lation instructions and go directly to the Quick
Start Guide on page 47.
The first step is to connect the focusing column Assembling the focusing column 2. Hold the incident-light to the focusing
to the corresponding base. 1. Lay the focusing column on the edge of a column and secure it to the base using the
table or another stable substrate so that three screws provided.
Tools used the three threads at the end of the column
★★ Allen key provided protrude over the edge.
Tools used
★★ Allen key provided
Tools used Assembling the Microscope Carrier 2. Tighten the screw using the Allen key provi-
★★ Allen key provided 1. Press the microscope carrier against the ded.
focusing column.
Tools used Installing the coaxial illuminator 3. Push the coaxial illuminator upwards and
★★ Allen key provided 1. Guide the coaxial illuminator to the tube tighten the screw on the coaxial illumina-
from the left or right. tor.
Tools used 1. Unscrew the fastening screw on the optics 3. Push the optics carrier upwards and tighten
★★ Allen key provided carrier. the fastening screw.
Tools used Removing the diaphragm ring Installing the fine focus
★★ Allen key provided 1. Unscrew the 3 fastening screws on the 1. Place the fine focus on the optics carrier.
diaphragm ring of the optics carrier.
mance
HR and DM objectives are high-perfor-
objectives and always cause
vignetting. This vignetting is not a malfunction.
Tools used 1. Push the tube into the dovetail ring and 2. While holding the tube only slightly, care-
★★ No tools required. rotate it slightly in both directions until the fully tighten the positioning screw. It is
positioning screw meshes with the guide automatically brought to the correct posi-
groove. tion.
Prerequisite for the installation
When using the ergo binocular tube or
another tube, the Y video/phototube
must be used.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.Assembling the tube
You can extend the overall magnification 2. Remove the plastic tube guard.
range using available 10×, 16×, 25× and
40× wide-field eyepieces for persons wearing
glasses.
carrier and tighten the three fastening The 2× objective must be installed on the
screws. centerable objective thread.
Unpacking the base Focusing drive and column 2. Attach your focusing drive column from
The base is delivered with the adapter plate 1. Unscrew the extension plate from the base below using the four hexagon-head screws.
installed. Make sure the instruments are unpa- using the Allen key provided.
cked on a flat, sufficiently dimensioned, and 3. Re-attach the adapter plate to its origi-
non-slip surface. nal position using the six hexagon-head
screws.
•
Before you can use the transmitted-
light base for the first time, it is abso-
lutely necessary to remove the two transport
anchors. Anchor of mirror
Standard delivery The selected stage is mounted on the base Standard stage
The base is delivered with the adapter plate before commissioning. You can switch between 1. Take the glass plate from the rectangular
installed. The selected stage (Leica IsoPro™ the stages at any time with just a few hand gap in the standard stage.
mechanical stage or Leica standard stage 10 movements.
447 269), and the focusing drive will have to be 2. Position the stage on the transmitted-light
mounted later. The following paragraph assumes use of the base in such way that the four holes align
base without the stage mounted. Disassembly over those in the base.
Stage assembly is performed in reverse order of the following
The Leica TL BFDF transmitted-light base can be steps. 3. Attach the stage to the base with the four
equipped with three different stages. supplied hexagon-head screws.
★★ Leica IsoPro™ manual mechanical stage 4. Insert the glass plate back into the standard
stage.
★★ Leica IsoPro™ automatic mechanical stage
Leica IsoPro™ Mechanical Stage Before the Leica IsoPro™ mechanical stage is Left or right operation
Before the Leica IsoPro™ mechanical stage is mounted to the base, the axis containing the If the controls are to be mounted on the left-
mounted to the base, the axis containing the control buttons is attached either on the left or hand side, the gear rod on the bottom side of
control buttons is attached either on the left or the right side of the mechanical stage. the mechanical stage must be unscrewed and
the right side of the mechanical stage. reattached in reverse.
1. Take the glass plate from the mechanical Control assembly Mechanical stage assembly
stage and turn it around. 1. Take the glass plate from the mechanical 1. Place the mechanical stage onto the base.
stage and turn it around.
2. Change the gear rod from the left to the 2. Pull the upper part of the mechanical stage
right-hand side. 2. Attach the axis with the control buttons to carefully towards the user, fixing the lower
the desired side. The fastener snaps into the part onto the transmitted-light base.
mechanical stage magnetically.
3. Attach the mechanical stage evenly to the
3. Attach the axis with the two supplied hexa- three threaded holes.
gon-head screws.
4. Now move the mechanical stage as far as it
4. Attach the cover rail to the mechanical will go in the direction of the column.
stage.
5. Insert the glass plate into the mechanical
stage.
Basics 1. Unpack the mechanical stage from the 2. Secure the motorized mechanical stage to
The transmitted-light bases of the Leica TL series transport packaging and position it on the the base using three M4 screws.
(TL BFDF, TL RC™, TL RCI™) are supplied with an transmitted-light base.
installed extension plate. The selected stage
(Leica IsoPro™ mechanical stage or standard stage
10 447 269) and the focusing drive will have to
be mounted later.
1
The motorized mechanical stage is a sensitive 2
precision instrument. During installation, avoid
subjecting the stage to impact or severe vibra-
tions.
3. Remove the two M3 screws and 4. Remove the two M4 screws and the sleeve 5. Remove the four shock-absorbing cartons
the holder from the mechanical stage. from the mechanical stage. from the mechanical stage.
•
The TL BFDF, TL RC™ and RCI™ transmitted-light Never move the sledge of the moto-
bases can be equipped with three different rized mechanical stage manually in
stages: Standard stage, manual and automated the X direction, as otherwise the mechanical
IsoPro™ mechanical stage. The selected stage is system will be damaged!
mounted on the base before commissioning.
You can switch between the stages at any time 1. Place the mechanical stage onto the base.
with just a few hand movements.
2. Pull the upper part of the mechanical stage
The following paragraph assumes use of the carefully towards the user and fasten the
base without the stage mounted. Disassembly lower part onto the transmitted-light base.
is performed in reverse order of the following
steps. 3. Attach the mechanical stage evenly to the
three threaded holes.
Mechanical stage for X-Y stage control As soon as all instruments have been plugged
module into the control box and it has been plugged
1. Plug the CTL2 plug of the mechanical stage into the power supply, the mechanical stage
motor into one of the three available CTL2 initializes and automatically drives to the mid
interfaces. position.
2. Plug the CTL2 plug of the Leica PSC control- 4. Connect the PC (where applicable) and X/Y
ler into another CTL2 interface. Stage DCI Module (using a suitable USB
cable).
★★ The third CTL2 interface is available for
connecting other instruments, such as the
Leica foot switch (10 447 398) or TL RCI™
transmitted-light base (10 446 352).
The A version of the Leica Z series features The terminals The connection to the PC and to other instru-
extensive automation options with which vari- ments is made using the terminals on the rear
ous microscope data and settings can be read side of the column:
out, transferred to the PC and reproduced later.
1. 2× CTL2 terminals for auxiliary equipment
1
3 such as the distribution box of the Z series,
the SmartTouch panel, the Leica TL RCI
base and other accessories from the Leica
2 product range.
The integrated cable duct in the column enab- Feeding the cables 3. Place the cables in the cable duct and screw
les a neat cable layout around the macroscope. 1. Unscrew the three screws on the cable the cover on tightly.
For example, the USB or FireWire cables of the duct.
camera can be stowed in the cable duct.
2. Remove the cover of the cable duct. Tip: Estimate the length of the cable ends you
will need before screwing on the cover. For
thick cables, it is difficult to change the length
retroactively.
Required tools Installing the Leica LED3000 NVI™ Alternative installation for manual columns
★★ None 1. Connect the Leica LED3000 NVI™ to the 1. Connect the external power supply unit (10
focusing column via the CTL2 connection if 450 266) to the Leica LED3000 NVI™.
the focusing column is equipped with inte-
1 Digital camera
2 ErgoTube™
1
3 Eyepieces
4 Adjustable eyepiece
2
tubes
5 Video/Phototube Y 3
6 Microscope carrier
7 Iris diaphragm 4
8 Optics carrier (zoom 5
body) 6 15
9 Click stops on/off 7
10 Zoom wheel 8 16
11 Fine focus
12 Objective 9
13 LED ring illuminator 10
14 incident-light 11
15 Microscope column 12
13
16 Focusing drive
14
An eyepiece not only makes it possible to look passively into the macro-
scope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10x and 40x.
The eyepieces form the connection between If you do not wear glasses: If you wear glasses:
the tube and the eye of the observer. Simply 1. Hold the eyepiece firmly and rotate the 1. Hold the eyepiece firmly and rotate the
push them into the tube and they are ready to eyecups forwards counterclockwise. eyecups clockwise towards the rear, as
use. otherwise the viewing distance is too
great.
Each eyepiece offers a certain magnification
factor that has a determinative effect on the
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical grati-
cules that enable measuring and quantifying of
specimens.
Dioptric Correction
A built-in dioptric correction is available for 2. If an eyepiece is equipped with the integ- 2. If an eyepiece is equipped with the integ-
eyeglass wearers. For more information, refer rated dioptric correction, turn the value to rated dioptric correction, turn the value to
to page 53. the "0" mark. the "0" mark.
The interpupillary distance is correctly set if you Adjusting the interpupillary distance 3. Slowly approach the eyepieces with your
see a single circular image field when looking 1. Look into the eyepieces. eyes until you can see the complete image
at a specimen. field without corner cutting.
2. Hold the eyepieces with both hands. Push
If you are still a novice macroscope user, you the eyepieces together or separate them
may need a short time to become accustomed until you see a circular image.
to this. Not to worry—after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece measu-
res approx. 22 mm for 10/23B wide-field eyepi-
eces for persons wearing glasses. ✗ ✓
Leica macroscopes are parfocally matched. A Using the Dioptric Correction Now, if you adjust the magnification from the
prerequisite for this the correct setting of the 1. Set the dioptric correction of both eyepieces lowest to the highest level, the specimen is
diopters. The following adjustments only have to the mid position ("0" diopter settings). always brought into sharp focus. If not, repeat
to be carried out once by each user. the process.
2. Look through the eyepieces and focus on a
Therefore, all Leica eyepieces are also available flat specimen. The system is now parfocally matched to your
with built-in dioptric correction, allowing the eyes.
macroscope to be used without glasses even 3. Rotate both eyepieces to the maximum
by those with vision problems. The correction value of "+5".
comprises ±5 diopter settings.
4. Hold one eye closed and rotate the other
eyepiece in the "-" direction until the speci-
men appears sharp.
Use 2. Clamp the reticule on the insert, applying 4. You can now align the reticule by rotating
Leica reticules make length measure- moderate pressure. Ensure that the grati- the eyepiece in the tube and then tighte-
ments and counting easier, particularly cule fits tightly. ning it using the clamping screw.
for workstations that are not equipped with a
digital camera and LAS software.
•
Direct contact with eyepieces is a
potential transmission method for
bacterial and viral infections of the eye. The
risk can be kept to a minimum by using indivi-
dual eyepieces or detachable eyecups. Eyecups
can be ordered separately. Please contact your
Leica partner.
• load of up to 15 kg.
★★ The outer, fine adjustment is used for fine 2. The coarse/fine adjustment carries a
★★ The focusing drive can be operated either focusing.
left- or right-handed.
ment is 1 µm.
The resolution of the coarse/fine adjust-
For most macroscope users, digital documen- Leica DFC cameras Leica Application Suite
tation has become an invaluable part of their However, if you require absolute control over The "Leica Application Suite", or "LAS" for short,
work. Research results can be presented in an the camera and need the capability for measu- is, as it were, the digital extension of the Leica
attractive manner; measurements on the digi- rement, evaluation and more in addition to Z series macroscopes. In addition to capturing
tal image provide clarity and, in conjunction photography, the digital Leica DFC cameras are images, it lets you control the macroscope, illu-
with the motorized IsoPro™ mechanical stage, exactly right for you. Together with the Leica minator, stages, cameras and more. For additio-
even images of large specimens can be captu- Application Suite, they provide virtually limit- nal information, refer to the LAS online help.
red step by step and automatically joined to less freedom of use. For additional informa-
create a new complete image. tion about Leica cameras, refer to the camera's
documentation.
Adapter
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. For this purpose,
Leica Microsystems offers a variety of adapters
that can be used together with the 50% and
100% trinocular tubes.
Application
All Leica DFC cameras are equipped with a Alternatively, you can also use a C-mount adap-
standardized C-mount interface. In turn, the ter with higher magnification. This primarily Cameras from third-party suppliers
C-mount adapter for the respective trinocular avoids the critical border area of the field of In addition to Leica DFC cameras with the stan-
tube is connected to this interface. This adapter vision and concentrates on the center of the dardized C-mount interfaces, you can connect
creates a solid mechanical connection between field of vision. third-party cameras such as single-lens reflex
the macroscope and camera and ensures opti- cameras to the macroscope using a T2 bayonet
mum rendering of the macroscopic image on Camera optimal suitable adapter. To do so, instead of the C-mount adap-
the image sensor of the camera. (large (smaller ter, simply use the corresponding SLR adapter
image field) image field) with T2 connection. However, these third-party
Usually, the ideal is for the digital camera to cameras are not integrated into the Leica Appli-
DFC295 0.5× 0.63×
capture as much of the field of vision as possi- cation Suite and have to be operated using
ble, while excluding as much of the black edge DFC420 0.5× 0.63× the corresponding software from the camera
of the field of vision as possible. To do so, the DFC490 0.63× 0.8× manufacturer.
magnification factor of the C-mount adapter DFC500 0.63× 0.8×
must match the image format of the sensor as The Leica digital cameras are detailed in a sepa-
closely as possible (see table). rate user manual along with instructions for
their assembly and use.
If there is unwanted shading at the corners even
with a compatible C-mount adapter installed, it
can be corrected using the "Shading function"
of the camera software.
Use
With its third beam path, the trinocular video/
phototube 50% enables you to simultaneously
view and photograph a specimen. The available
light is divided as follows:
Assembly
Fasten the "trinocular tube 50%" to the optics
carrier instead of the ErgoTube™ (refer also to
page 28).
Use Switchover
With its third beam path, the trinocular video/ ★★ Turn the controller on the right side of the
phototube 100% enables you to either view or tube into the horizontal position in order to
photograph a specimen. This means that 100% guide all available light into the eyepieces.
of the light is available to one or the other beam You can now observe the specimen.
path. The other beam path remains opaque or
black. ★★ Turn the controller on the right side of the
tube into the vertical position in order to
Assembly guide all available light into the camera.
Fasten the "trinocular tube 100%" to the optics You can now photograph the specimen.
carrier instead of the binocular ErgoTube (refer
also to page 28).
To meet the various requirements regarding ★★ Also available are the M series 0.63×, 0.5×, Achromatic objectives with a long focal
imaging properties, there is a choice of high- 0.32× achromatic objectives, which can be length
quality interchangeable planachromatic and attached to the zoom or the fine focusing For special applications, achromatic objectives
planapochromatic objectives and also lower- using an adapter.You can attach the vertical with long working distances and focal lengths
priced interchangeable achromatic objectives. illuminator or the attachment for incident/ of f=100 mm to 400 mm are available.
You can use the objectives on the Z6 APO or Z16 oblique observation to the achromatic
APO zoom systems or with the fine focusing. objective.
2
1 2 3
3
Extension plate of the
4
transmitted-light base TL ST Rear side of the transmitted-light base TL ST
1 Screws for changing the halogen lamp
2 Power connection socket
1 Adapter plate for easy assembly of focusing 3 Power switch
drives
2 Removable glass plate
3 Controller for light intensity
4 Adjustment for deflection mirror
•
Before you change the lamp, it is abso- 1. Unscrew the two screws on the heat sink When inserting the lamp, ensure that
lutely necessary to unplug the power and pull the heat sink out, along with the the cables are inside the two metal
plug from the base to prevent the risk of elec- lamp. clamps. This prevents the cables from getting
tric shock! caught during insertion.
ces the service life of the lamp! 2. Carefully pull out the lamp and mount by
pulling them upwards.
•
Observe the user manual—in parti- Bright field is suitable for examining translu- In dark-field illumination, a ring illuminator is
cular, all safety regulations—from the cent objects featuring contrasting structures. used in such a way that the direct light
manufacturers of the light guide and cold light The object is directly illuminated from below does not reach the objective without a speci-
source. and is seen in its natural colors against a bright men. Only the structure of semitransparent,
background. opaque objects disperses the light, making the
★★ Switch on the cold light source according to object visible against a dark background.
the manufacturer's user manual and adjust ★★ Turn the control as far as it will go towards
the brightness. "BF" ("bright field"). ★★ Turn the control as far as it will go towards
"DF" ("dark field").
Transmitted-light control
The Leica TL BFDF transmitted-light base has
a control that switches the light from "bright
field" to "dark field".
Fingertip with bright field illumination Identical subject with dark field illumination
1 2
1
2
3
4
5
6
3 45 6
7
Vertical column - transmitted-light base adapter plate
1 Power switch
2 Power connection socket
1 Heat sink of the integrated halogen illumina-
3 USB socket, type B
tion (only TL RCI™)
4 USB socket, type A
2 Extension plate for focusing drives
5 2 CAN bus
3 Standard stage 10 447 269
6 Screws for changing the halogen lamp
4 Filter holder
5 Control of top and bottom flaps of the
Rottermann Contrast™
6 Button for mirror and horizontal movement
of the mirror
7 Transmitted-light base
The concave cutout on the handle indicates the Functions of the rotary knob
concave side of the mirror, allowing intuitive The rotary knob (1) fulfills the following tasks:
operation at any time without eye contact.
★★ Turning the deflection mirror from the flat
The angle of light incidence in the specimen to the concave side
plane changes depending on the tilt and posi-
tion of the mirror. As a result, switching between ★★ Slight tilting to guide the light beam
transmitted-light bright field, oblique illumina- through the specimen plane at a steeper or
1
tion and dark field-like illumination is possible. flatter angle
The transmitted-light base TL RCI™ has two Using a USB mouse (only TL RCI™)
electronic potentiometers that control the color The Leica USB mouse controls the CCIC™ and
intensity (1) and color temperature (2). dimming function of the TL RCI™ base. Connect
the mouse to the corresponding USB port of
The controller for the color temperature simul- the base.
taneously serves as an electronic shutter:
★★ The scroll wheel of the mouse is occupied
★★ To interrupt your work, click the potentio- by default with the CCIC™ control system
meter (2). and is used to control the illumination
intensity.
1 2
★★ Click again to switch on the illumination.
The electronics returns the color tempera- ★★ To switch the illumination on or off, briefly
1 Potentiometer for controlling the CCIC™ ture to the previously configured settings. click the scroll wheel.
(Constant Color Intensity Control) illumina-
tion intensity ★★ To enter or exit dimming mode, click and
2 Potentiometer for controlling the color hold down the scroll wheel for longer than
temperature 2 seconds.
Setting the relief contrast Depending on the flap position, a part of Increased contrast without relief is attained if
The two switches on the left side of the trans- the opening of the built-in Fresnel lenses are both diaphragms are set to 45°. A gap-like illu-
mitted-light base TL RC™/TL RCI™ activate two covered, which results in the different contrast minated area is created. By tilting the deflection
built-in flaps. The outer switch (1) controls the effects. Phase structures typically act as spatial, mirror slightly, you can move the gap over the
inverted relief contrast, and the inner switch (2) relief-type images—in the positive relief cont- entire field of view and quickly toggle between
adjusts the positive relief contrast. rast like hills, in the inverted relief contrast like positive and negative relief images. The dyna-
valleys. mic effect makes it easy to distinguish phase
structures from amplitude structures.
(refractive index of the environment) and the
perception of the viewer, it is possible that the
switches described below for positive and inver-
ted relief contrast are to be operated in reverse.
This means that the lower switch, rather than
the upper switch, controls the inverted relief
contrast and vice-versa.
1 2 3
Filters for Leica TL RC™ and TL RCI™ 2. Take the empty filter from an available filter Daylight filter for Leica TL ST
The transmitted-light bases TL RC™ and TL RCI™ slot in the filter holder. A daylight filter is also available for the Leica TL
can be equipped with up to three filters— ST transmitted-light base.
available as accessories—simultaneously. By 3. Insert the desired filter.
customer request, the filters are also available
as one-off items.
1 2
1
1 2
2
1 2 3 4
adjusted in 10 increments.
The intensity of the illuminator can be Use 2. Adjust the brightness by briefly pressing
•
The light of the Leica LED3000 NVI™ can be the or buttons.
very bright. Therefore, always switch on the
illuminator before you look through the eyepi-
The Leica LED3000 NVI™ can also be
controlled via the Leica Application Suite eces!
(LAS) or the Leica SmartTouch.
1. Switch on the illuminator by briefly pres-
sing the (ON/OFF) button.
between 50 mm and 150 mm.
The supported working distance is
Connection
Connect the Leica PSC controller and the Leica
IsoPro™ motorized cross-stage to the DCI box.
Focusing Information about the focus positions Saving the focus positions
Turning counterclockwise = downwards ★★ Focus positions can be stored using the 1. Focus on the first specimen detail.
Turning clockwise = upwards manual control or on the computer.
2. Press the red switch for at least 1.5 seconds.
★★ A total of up to 5 focus positions can be Short beeps provide confirmation.
Toggle between stored.
coarse and fine focusing 3. Focus on the next specimen details and
1. Press the black button briefly. ★★ If a sixth focus position is stored, the one save these positions also.
nearest to it will be deleted.
★★ Each focus position can be canceled indivi- Moving to stored focus positions
dually. 1. Press the red switch briefly.
When the system is switched on, the stored focus positions.
coarse focus is always switched on also.
the footswitch.
1 Focus positions cannot be stored using
2
1 Upwards
2 Downwards
Leica Z6 APO
a Z6 APO
Koaxial-Auflichtgehäuse
Leica Z6 APO / Z16 APO User Manual 94
Objectives
Ø43
0.5
Lowest
Focusing
25
Position
37
71
24
25.5
Interface
117
60
Ø76
Ø87
10
40
54
77.5
Highest
Focusing
Positio
101
69
25
25
25
Lowest
Focusing
Position
Ø76
60
Ø87
25
Interface for Ø25 Column
∅ 45 mm
M42
(Fine Focus Interface)
∅ 40 mm
6 mm
11 mm
8 mm
M60
(Achromat Objective Interface)
∅ 66 mm
0.57 7.1 32.3 3.6 64.6 5.7 40.4 14.3 16.1 35.6 6.5
0.8 10 23.0 5 46.0 8 28.8 20 11.5 50 4.6
1 12.5 18.4 6.3 36.8 10 23.0 25 9.2 62.5 3.7
1.25 15.6 14.7 7.8 29.4 12.5 18.4 31.3 7.4 78.1 2.9
1.6 20 11.5 10 23.0 16 14.4 40 5.8 100 2.3
2 25 9.2 12.5 18.4 20 11.5 50 4.6 125 1.8
2.5 31.3 7.4 15.6 14.7 25 9.2 62.5 3.7 156.3 1.5
10×/23B
3.2 40 5.8 20 11.5 32 7.2 80 2.9 200 1.2
3.6 45 5.1 22.5 10.2 36 6.4 90 2.6 225 1.0
4 50 4.6 25 9.2 40 5.8 100 2.3 250 0.9
5 62.5 3.7 31.3 7.4 50 4.6 125 1.8 312.5 0.7
6.3 78.8 2.9 39.4 5.8 63 3.7 157.5 1.5 393.8 0.6
8 100 2.3 50 4.6 80 2.9 200 1.2 500 0.5
9.2 115 2.0 57.5 4.0 92 2.5 230 1.0 575 0.4
0.57 11.4 21.1 5.7 42.1 9.1 26.3 22.8 10.5 57 4.2
0.8 16 15.0 8 30.0 12.8 18.8 32 7.5 80 3.0
1 20 12.0 10 24.0 16 15.0 40 6.0 100 2.4
1.25 25 9.6 12.5 19.2 20 12.0 50 4.8 125 1.9
1.6 32 7.5 16 15.0 25.6 9.4 64 3.8 160 1.5
2 40 6.0 20 12.0 32 7.5 80 3.0 200 1.2
2.5 50 4.8 25 9.6 40 6.0 100 2.4 250 1.0
16×/15B
3.2 64 3.8 32 7.5 51.2 4.7 128 1.9 320 0.8
3.6 72 3.3 36 6.7 57.6 4.2 144 1.7 360 0.7
4 80 3.0 40 6.0 64 3.8 160 1.5 400 0.6
5 100 2.4 50 4.8 80 3.0 200 1.2 500 0.5
6.3 126 1.9 63 3.8 100.8 2.4 252 1.0 630 0.4
8 160 1.5 80 3.0 128 1.9 320 0.8 800 0.3
9.2 184 1.3 92 2.6 147.2 1.6 368 0.7 920 0.3
0.57 17.8 13.3 8.91 26.7 14.3 16.7 35.6 6.7 89 2.7
0.8 25 9.5 12.5 19.0 20 11.9 50 4.8 125 1.9
1 31.3 7.6 15.6 15.2 25 9.5 62.5 3.8 156 1.5
1.25 39.1 6.1 19.5 12.2 31.3 7.6 78.1 3.0 195 1.2
1.6 50 4.8 25 9.5 40 5.9 100 2.4 250 1.0
2 62.5 3.8 31.3 7.6 50 4.8 125 1.9 313 0.8
2.5 78.1 3.0 39.1 6.1 62.5 3.8 156 1.5 391 0.6
25×/9.5B
3.2 100 2.4 50 4.8 80 3.0 200 1.2 500 0.5
3.6 113 2.1 56.3 4.2 90 2.6 225 1.1 562 0.4
4 125 1.9 62.5 3.8 100 2.4 250 1.0 625 0.4
5 156 1.5 78.1 3.4 125 1.9 313 0.8 781 0.3
6.3 197 1.2 98.4 2.4 158 1.5 394 0.6 984 0.2
8 250 1.0 125 1.9 200 1.2 500 0.5 1250 0.2
9.2 288 0.8 144 1.6 230 1.0 575 0.4 1438 0.2
0.57 28.5 8.4 14.3 16.8 22.8 10.5 57 4.2 143 1.7
0.8 40 6.0 20 12.0 32 7.5 80 3.0 200 1.2
1 50 4.8 25 9.6 40 6.0 100 2.4 250 1.0
1.25 62.5 3.8 31.3 7.7 50 4.8 125 1.9 313 0.8
1.6 80 3.0 40 6.0 64 3.8 160 1.5 400 0.6
2 100 2.4 50 4.8 80 3.0 200 1.2 500 0.5
2.5 125 1.9 62.5 3.8 100 2.4 250 1.0 625 0.4
40×/6B
3.2 160 1.5 80 3.0 128 1.9 320 0.8 800 0.3
3.6 180 1.3 90 2.7 144 1.7 360 0.7 900 0.3
4 200 1.2 100 2.4 160 1.5 400 0.6 1000 0.2
5 250 1.0 125 1.9 200 1.2 500 0.5 1250 0.2
6.3 315 0.8 158 1.5 252 1.0 630 0.4 1575 0.2
8 400 0.6 200 1.2 320 0.8 800 0.3 2000 0.1
9.2 460 0.5 230 1.0 368 0.7 920 0.3 2300 0.1
Data with Leica DFC490 digital camera / 1× planapochromatic objective / AS tube / 0.63× video objective
Magnification
Chip: Object 0.36× – 2.3× 0.36× – 5.8×
Digital resolution* 33.3 – 210 Lp / mm 33.3 – 336 Lp / mm
24.5 mm × 18.4 mm / 24.5 mm × 18.4 mm /
Object field projected onto chip 3.9 mm × 2.9 mm 1.5 mm × 1.14 mm
Depth of field (diaphragm open) 1.06 mm – 0.03 mm 1.4 mm – 0.03 mm
Depth of field (dia. closed) 10.7 mm – 0.26 mm 10.9 mm – 0.3 mm
ErgoTube® and ErgoModule® are registered in the United States Patent and Trademark Office
Illumination types
Bright field Yes
Dark field Yes (single-sided)
Oblique light No
Relief Contrast System (RC™) No
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control No
Integrated filter holder Yes
Coated optics for increasing the color Yes
temperature
Matching of high num. aperture No
Remote control options No
AntiShock™ Pads Yes
Dimensions (W×H×D) 340×430×85 mm
Illumination types
Bright field Yes
Dark field Yes
Oblique light No
Relief Contrast System (RC™) No
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control Yes*
Integrated filter holder No
Coated optics for increasing the color No
temperature
Matching of high num. aperture Yes**
Remote control options Yes***
AntiShock™ Pads Yes
Dimensions (W×H×D) 340×390×90 mm
*With cold light source Leica CLS150 LS **Concave mirror *** With external light source
Illumination types
Bright field/dark field Yes/yes Yes/yes
Oblique light/Relief Contrast System (RC™) Yes/yes Yes/yes
CCIC (Constant Color Intensity Control) No Yes
Internal shutter/lamp control Yes** Yes
Integrated filter holder Yes Yes
Coated optics for increasing the color temperature Yes Yes
Matching of high num. aperture Yes*** Yes***
Remote control options Yes**** Yes
AntiShock™ Pads Yes Yes
Dimensions of base (W×H×D) 340×390×95 mm 340×440×95 mm
*Single-sided **With cold light source Leica CLS150 LS ***Concave mirror ****With external light source
Parameter Example
MO Magnification of the objective MO 1× objective
ME Magnification of the eyepiece ME 25×/9.5 eyepiece
z Magnification of the changer position z Zoom position 4
q Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45° q Coaxial incident light 1.5×, tube factor
ErgoTube™ r Factor 1.25×
r Factor of 1.25× when using video/phototube Y
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Calculation example: Magnification in the binocular tube: Calculation example: Field of view diameter in the specimen
★★ Keep accessories in a dust-free place when ★★ Remove dust with a pneumatic rubber bulb
not in use. or with a soft brush.