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Pharmacognosy Activity Carbohydrates Different Types of Starch Powder

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PHARMACOGNOSY ACTIVITY

CARBOHYDRATES

Different Types of Starch Powder


Background Starch (Amylum) is a complex polysaccharide with very high molecular weight and consists of large number
of monosaccharide units linked together through glycosidic linkage. Starch is a natural plant product which is a mixture
of amylose (25%) and amylopectin (75%).
Amylose: - Linear molecule consists of 250 – 300 glucose units of α – D – glucose units linked together through α – 1, 4
glycosidic linkage. - More water soluble than amylopectin. - Amylose + Iodine (I2)= Blue color
Amylopectin: - Branched molecule consists of more than 1000 units of α – D – glucose units linked together through α –
1, 4 and α – 1, 6 glycosidic linkage. - Less water soluble than amylose. - Amylopectin + Iodine (I2) =Violet color S

Sources of starch
Starches are obtained from maize (Zea mays L.) (Graminae), wheat (Triticum aestivum L. (Graminae), rice (Oryza sativa
L.) (Graminae), and potato (Solanum tuberosum L.) (Solanaceae).
Properties of Starch
- White mass powder, odourless with starchy taste.
- Insoluble in water (form colloidal solution).
- Starch + Iodine (I2) Deep blue color.
- Starch + H2O gel (with heat).
- Corn and wheat starches have neutral pH, rice starch has slightly alkaline pH, and potato starch has slightly acidic pH.

Measurement of starch are necessary for the quantitative identification of closely allied substances. In most cases, the
original drugs are adulterants or substitutes. By comparing the sizes of starch grains in different species, the adulterants
and/or substitutes in crude drugs can be distinguished using optical microscopy

Procedure:
- Observe the physical characters (colour, odour, texture and taste) of maize, wheat, rice and potato starches. -
Observe the solubility of starch in water before and after heating. - Determine the pH of starch: mix 0.2 g of starch
with 5 ml water, dip in a piece of pH paper and read the pH.

Fill the results for these physical and identification tests in the following table:
CORN STARCH WHEAT STARCH RICE STARCH POTATO STARCH
COLOUR WHITE WHITE TO OFF- WHITE WHITE
WHITE
TEXTURE GRITTY FINE GRANULARITY FINE TEXTURE
WITH GRANULES
ODOR CEREAL ODOR CEREAL ODOR EARTHLY ODOR
TASTE Tastes like flour or NEUTRAL TASTE NEUTRAL TASTE NEUTRAL TASTE
none at all
SOLUBILITY IN COLD INSOLUBLE
WATER
SOLUBILITY IN HOT
WATER

Microscopical examination:
• Prepare amount of starch in water (half spatula of starch on a clean slide and add 2- 3 drops of water).
• Smear the paste on the slide using cover slip to make a thin smear.
• Observe the starch granules and identify the structure of different starch grains by microscope using 10X and 40X.

Draw the microscopically features of the following:

1. Corn starch

2. Wheat starch

3. Rice starch

4. Potato starch

5. Maize starch
1. Corn starch

2. Wheat starch
3. Rice starch

4. Potato starch

5. Maize starch

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